The invention relates to a method for measuring tobacco-specific nitrosamines (TSNAs for short) by using a super efficient liquid chromatogram-tandem
mass spectrum
combined method, and belongs to a method for detecting TSNAs. The method comprises the following steps of: adding
internal standard-containing
aqueous solution of
ammonium acetate into a filter sheet after
trapping smoke and cigarette filters smoked completely under standard conditions or tobacco shreds, performing ultrasonic extraction, transferring 1 to 2mL of extract into a small
solid-phase extraction column taking N-vinyl pyrrolidone-
benzene sulfonic acid copolymer as filler, flushing the small column by using
aqueous solution of methanoic acid and
methanol, finally eluting the small column by using solution of aqueous
ammonia and
methanol solution, analyzing the eluant by using an ultra performance liquid
chromatography-
mass spectrometer /
mass spectrometer (UPLC-MS / MS), and quantifying according to the
area ratio of a component peak to an
isotope internal standard peak. The detecting method can be used for detecting the TSNAs in cured and
hybrid cigarette mainstream
smoke, cigarette side-
stream smoke, filter tip intercepting smoke and tobacco shreds, and has the advantages of simple pretreatment, accurate quantification, high instrument analysis speed, low
detection limit, high sample treatment flux and the like.