37results about How to "Quantitatively accurate" patented technology

The invention discloses a three-dimensional laser post-ionization-secondary neutron particle mass spectrometer which comprises a vacuum pool, a sample introduction system, a primary ion emission system, a three-dimensional laser post-ionization system, an ion optical system, a time-of-flight mass spectrometer, an observation system and a computer control system. By adjusting the wavelength, the frequency and the energy value of the laser, resonance between the energy of laser photons and the electron energy level of target molecules can be realized to realize selective ionization of substances, and the method has extremely high element, nuclide and molecule selectivity, can provide the sensitivity of an instrument, and can be used for mass spectrometry. The device can be applied to single-cell imaging analysis test of lipid, metabolites, small-molecule drugs and part of elements with the molecular weight smaller than 1000 Da, the problems of space metabolome small-molecule metabolite test and ultrahigh spatial resolution imaging are solved, and the device can be independently used as an SIMS when laser post-ionization is not used.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The application belongs to the technical field of detection, and particularly relates to a method for determining the content of chlorhexidine acetate in a disinfectant. The method for determining the content of chlorhexidine acetate in the disinfectant comprises the following steps: (1) dispersing the sample with glacial acetic acid, releasing chlorhexidine acetate combined with sample matrix components, and extracting with a methanol aqueous solution; and (2) taking a tetrabutylammonium bromide-acetic acid-acetonitrile solution as a mobile phase, and detecting with a high-performance liquid chromatograph. The method for detecting chlorhexidine acetate is simple in operation, good in reproducibility, complete in separation from impurities, high in accuracy, and has a good application prospect.

The invention discloses a label printer core, and relates to the technical field of printer cores. The device comprises a base body and an upper cover body rotationally connected to the upper end of the base body, and further comprises a connecting rotating shaft arranged at the upper end of the base body; the thermal transfer ribbon conveying assembly is arranged in the upper cover body; the driving motor is fixed in the base body, and one side in the base body is transversely and rotationally connected with a paper pressing roller; the linkage mechanism is arranged at the output end of the driving motor; the paper thickness quantitative adjusting mechanism is arranged on the inner side of the upper cover body. Through the linkage rod, the upper cover body can be locked and limited through the first buckle teeth and the second buckle teeth, the current-conducting plate is arranged at one end of the linkage rod and is grounded, the thermal transfer ribbon is attached to the outer side of the linkage rod, static electricity on the thermal transfer ribbon is guided out through the current-conducting plate by the linkage rod, and the static electricity on the thermal transfer ribbon is discharged through the current-conducting plate. The printing quality of the printing head can be improved, the service life of the printer can be prolonged, the arranged PCB body can be detached and replaced, and the PCB body can be used independently and can also be used separately.

The utility model discloses a quantitative strain packaging connector which comprises a stocker used for storing quantitative strains; the water storage device is used for storing quantitative clear water; the connector is used for connecting the material storage device and the water storage device; a connecting cavity is formed in the connector, the water storage device is located above the material storage device, and after the water storage device moves relative to the connector, the water storage device is communicated with the connecting cavity, so that clear water in the water storage device flows into the material storage device; the device has the advantages of being compact in structure, accurate in quantification, convenient to operate and excellent in sealing performance.

This invention discloses a wide dynamic range substrate reagent, belonging to the field of biological detection reagent technology. The reagent consists of the following components: citric acid, 9.6 g / L; 3,3',5,5'-tetramethylbenzidine, 0.29 g / L; 3% hydrogen peroxide solution, 3 mL / L; 1 g / L polyvinyl alcohol; 0.5 g / L copovidone W-735; 0.5 g / L dimethyldiallylammonium chloride-acrylamide copolymer; 1 g / L PVP K30; and Tween 20, 0.005 g / L. mL / L; polyethylene glycol 10g-50g / L; pure water, balance. This invention, by adding specific polyethylene glycol and synergistically combining with other components, significantly reduces the reagent background signal and improves detection sensitivity; effectively broadens the detection dynamic range and increases the detection upper limit of high-concentration analytes; improves the linear correlation between signal and concentration; and enhances the thermally accelerated stability of the reagent. This reagent has comprehensive performance and is especially suitable for ELISA detection systems with high sensitivity and wide detection range.

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The invention discloses a headspace-gas chromatography detection method for 1, 3-butadiene in an automotive interior material, which comprises the following steps: 1) crushing a sample into fine particles, freezing with liquid nitrogen, embrittling, and crushing at low temperature; and (2) weighing a sample which is recovered to room temperature after being crushed, putting the sample into a headspace bottle, quickly sealing, and carrying out gas chromatography detection in a headspace sampling mode. On the basis of the headspace-gas chromatography technology, by optimizing headspace sampling conditions, instrument parameters and chromatographic column selection, the 1, 3-butadiene detection method which is simple and convenient in pretreatment, high in sensitivity and accurate in quantification is established. And the method is precisely positioned on a specific and complex substrate, namely an automotive interior material. Compared with the prior art, the method is simple and convenient to operate and high in detection speed, can effectively avoid the problems of incomplete dissolution, difficulty in sample introduction and the like, is suitable for measuring an actual sample, and provides a reliable technical means for detecting the residual quantity of 1, 3-butadiene in the automotive interior material.

This invention provides a method for detecting tetrachloronaphthalene, a persistent organic pollutant in eel products. The specific steps include: eel sample preparation, target analyte extraction and matrix purification, followed by analysis using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (UPLC-APCI-MS / MS). The measured peak area, fractionation ratio, and other relevant parameters are substituted into a specific formula to calculate the measured value of tetrachloronaphthalene in the test solution. This method fills the gap in my country's technology for detecting tetrachloronaphthalene-like persistent organic pollutants in eel products. It effectively eliminates interference from the complex matrix of eel products, accurately determines four tetrachloronaphthalene isomers in trace amounts, and possesses significant advantages such as high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

The invention provides a multi-sensing module combined hydrogen detection method and system under a room temperature condition, and relates to the technical field of hydrogen sensors, and the method comprises the following steps: obtaining output signals of a first sensing module and a second sensing module in real time; when the signal value output by the first sensing module is higher than a preset safety value, a first alarm is given; when the response rate of the output signal of the first sensing module is higher than the preset response rate value, sealing of the second sensing module is relieved, and a second alarm is given out according to the output signal value of the second sensing module. According to the invention, seamless and graded monitoring of hydrogen from low concentration to ultrahigh concentration can be realized, room temperature stable induction during low-concentration hydrogen leakage can be ensured, and accurate quantification can be carried out during high-concentration hydrogen leakage; the high-value sensing module is always in a sealed state to effectively protect the value, the low-value sensing module adopts indium doped molybdenum oxide nanobelts to ensure long-time stable use at room temperature, and the two sensing modules are combined and complemented, so that the hydrogen detection safety is higher.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

This utility model discloses a wastewater treatment agent quantitative dosing device and a wastewater tank, comprising a multi-axis displacement unit, a quantitative dosing unit connected to the moving end of the multi-axis displacement unit, and a control unit. The quantitative dosing unit includes a storage component, a dosing pump, a quantitative component, and a spraying component interconnected by pipelines. The quantitative component includes a quantitative tank and a float plate disposed within the quantitative tank. The float plate divides the quantitative tank into an upper chamber and a lower chamber. The lower chamber connects to the dosing pump and the spraying component. A fixed sensor and a loading component corresponding to the sensor are disposed in the upper chamber. The loading component is connected to the float plate. The control unit is electrically connected to the sensor, the dosing pump, and the spraying component. This utility model improves the efficiency and quality of wastewater treatment and reduces the risks and costs of manual operation.

The utility model relates to the technical field of coffee machines, in particular to a coffee machine with a weighing quantitative control module, which comprises a coffee machine body, the weighing quantitative control module is arranged on the coffee machine body, and the weighing quantitative control module comprises an independent support, a weighing platform, a pressure sensor and a main control circuit board. The pressure sensor is electrically connected with the main control circuit board, the weighing platform and the pressure sensor are installed at the upper end and the lower end of the independent support respectively, and a waste water disc assembly is arranged on the weighing platform. According to the utility model, the weighing platform and the pressure sensor are fixed on the independent support, and the waste water disc assembly is adjusted to be only contacted with the weighing platform instead of being contacted with the base instead of being normally contacted with the base, so that the weighing effect can be achieved no matter whether the waste water disc assembly is taken away or not; and the whole machine is controlled by the main control circuit board to finish extraction, so that accurate and quantitative control is achieved, and the manufacturing cost is lower.

The invention relates to the technical field of treatment drug monitoring, in particular to a method for simultaneously determining the concentration of multiple antiepileptic drugs in plasma. Comprising the following steps: S1, preparing a standard working solution; s2, preparing an internal standard solution; s3, mixing the standard working solution with blank plasma, adding an internal standard solution, centrifuging, and detecting to obtain a chromatogram of a standard solution; s4, based on the chromatogram, taking the ratio of the peak area of the anti-epileptic drug to the peak area of the corresponding internal standard substance as a vertical coordinate Y, taking the ratio of the concentration of the anti-epileptic drug to the concentration of the corresponding internal standard substance as a horizontal coordinate X, and fitting to obtain a standard curve equation; s5, preparing a plurality of groups of quality control working solutions, and verifying the standard curve equation obtained in the step S4; and S6, uniformly mixing the blood to be detected with the internal standard solution, centrifuging, detecting the supernate, and calculating the concentration of the antiepileptic drug in the plasma. The method can realize simultaneous, accurate and quantitative detection of multiple antiepileptic drugs in blood.

The invention relates to a dielectric wetting valve micro-fluidic chip for metabolic marker detection and a preparation method of the dielectric wetting valve micro-fluidic chip. A chip middle layer is stacked above a chip lower layer, and an inverted chip upper layer is stacked above the chip middle layer; the chip middle layer comprises a double-sided adhesive layer and a trident micro-channel, the periphery of the trident micro-channel is coated with the double-sided adhesive layer, the trident micro-channel comprises a central area, a first branch channel, a second branch channel and a third branch channel, and the first branch channel, the second branch channel and the third branch channel are uniformly distributed on the periphery of the central area; the head end of the first branch channel, the head end of the second branch channel and the head end of the third branch channel are respectively connected with the central area. Through the trident micro-channel, the detection of various metabolic markers becomes simpler, more convenient and more sensitive, and the detection precision of the metabolic markers is improved. According to the invention, through the trifurcate micro-channel, the sequential analysis of various targeted metabolites can be realized, an external pump is not needed, and the device has the advantages of high portability, accurate fluid control and the like.

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

The invention discloses a reagent containing a porcine pseudorabies virus gB protein magnetic particle coating substance, a preparation method and a kit, and relates to the technical field of antibody detection. The preparation method of the reagent containing the porcine pseudorabies virus gB protein magnetic particle coating substance comprises the following steps: incubating magnetic particles and a gB protein antibody in a coating buffer solution; the reaction liquid is removed, and the gB protein magnetic particle coating and first confining liquid are subjected to first confining; after closing is completed, the first closing liquid is washed away, and the gB protein magnetic particle coating and second closing liquid are subjected to second-time closing; after closing is completed, the second closing liquid is washed away, resuspension is conducted, and the reagent containing the gB protein magnetic particle coating substance is obtained. The magnetic particle coating containing the porcine pseudorabies virus gB protein is prepared by adopting a two-step sealing method, the sealing effect is good, and non-specific adsorption in a system is reduced. The kit provided by the invention contains the reagents and has the advantages of high analysis sensitivity, wide linear range and high accuracy.

The present invention discloses a method for preparing chloroquine. Polyphenol oxidase is added to a chlorogenic acid solution, oxygen is introduced, and chloroquine is prepared after mixed oxidation under water bath conditions of 25 to 38°C. The chloroquine is quenched with a nucleophilic reagent sodium benzenesulfinate to form a stable binding product. The working curve is drawn by ultra-high performance liquid chromatography-mass spectrometry, and the binding product is qualitatively and quantitatively detected to replace the detection of quinone substances. The purity of the prepared sodium benzenesulfinate of chloroquine reaches more than 95%, which can be used for quantitative determination. In addition, the preparation method of chloroquine in the present invention is low in cost, simple and convenient, easy to operate, and is conducive to popularization and use.

This invention discloses an antioxidant low-density lipoprotein monoclonal antibody and its applications, belonging to the field of antibody technology. The antioxidant low-density lipoprotein monoclonal antibody of this invention possesses high specificity, high affinity, and high sensitivity. An immunoturbidimetric assay kit prepared based on the antioxidant low-density lipoprotein monoclonal antibody of this invention can quantitatively detect oxidized low-density lipoprotein in peripheral blood samples, and can have a good diagnostic effect on cardiovascular diseases caused by atherosclerosis (AS).

The utility model provides a kind of oil dropping device for high-power oil lens microscopic imaging, including support adjustment module, oil storage module and oil inlet module, and oil inlet module and oil storage module are fixedly installed on support adjustment module;Oil inlet module includes quantitative pump, oil pipe, oil dropping needle and oil dropping needle fixer, and quantitative pump is fixedly installed on the fixed plate that support adjustment module has;Oil dropping needle is fixedly installed on the adjusting support that support adjustment module has by oil dropping needle fixer, and adjusting support is used to adjust the position of oil dropping needle, and oil dropping needle, quantitative pump and oil storage module are sequentially connected by oil pipe.This utility model ensures the quantity of each oil drop consistent by quantitative pump assembly, can realize accurate quantitative oil drop, avoids the problem that manual operation oil drop quantity is not accurate, and through support adjustment module, the position of oil dropping needle can be flexibly adjusted, and the position of oil dropping needle can be adjusted according to actual needs, to adapt to the observation needs of different samples.

The utility model relates to a kind of dry-mixed mortar automatic quantitative packing packing device, including rack, hopper and support plate, hopper is installed at the top of rack, support plate is set on rack by elastic support structure, the bottom of hopper is separately provided with solenoid valve and mechanical valve, rack is provided with instantaneous skewing mechanism, instantaneous skewing mechanism and mechanical valve are mutually matched, and instantaneous skewing mechanism is connected with elastic support structure by transmission structure, rack is horizontally symmetrical between hopper and support plate and is provided with infrared emitter and infrared receiver, the utility model, by setting elastic support structure below support plate, and it is connected with instantaneous skewing mechanism and mechanical valve, the accurate control of mechanical valve is realized, infrared emitter and infrared receiver are set between hopper and support plate of device, realize the opening and closing of solenoid valve, prevent the leakage of dry-mixed mortar when replacing container, reduce the pollution to working environment, improve production efficiency and product quality.

The present invention belongs to the field of sensor technology, and relates to a biosensor for detecting ovarian cancer exosomes by chemiluminescence method based on logic gate response. A chemiluminescence biosensor is constructed based on the specific recognition of aptamers corresponding to ovarian cancer exosome proteins, rolling circle amplification reaction, and the catalytic activity of G4 / heme DNA enzyme. The sensor has the advantages of wide detection range, low detection limit, high specificity, etc., which can make up for the defects and deficiencies of existing detection methods in early diagnosis of ovarian cancer and realize rapid and accurate quantitative detection of ovarian cancer.

The utility model provides a kind of laboratory liquid quantitative measurement and canning device, comprising: intercommunication quantitative component and switch component;Wherein, the quantitative component, comprising, hollow cylindrical can body (1), the can body (1) bottom is connected with the quantitative component top;Variable capacity adjusting rod (2) is equipped in the can body (1), for adjusting the hollow cavity volume of the can body (1);The switch component, comprising, shell (8), the shell (8) top is connected with the can body (1) bottom;Switch device is equipped in the shell (8), the hollow cavity of the switch device top is communicated with the can body (1), bottom is worn out the shell (8) bottom, for discharging the liquid inside the hollow cavity, the switch device side is worn out the lateral wall of the shell (8), for transporting liquid to the inside of the hollow cavity.

The invention discloses a detection primer and a detection method for picocyanobacteria. The invention discloses a specific primer designed for the 16S-23S rRNA ITS sequence of the marine picocyanobacteria "Syn / Pro" group, named Picocya-Ala-F / Picocya-boxA-R. The primer of the invention can cover the cyanobacteria groups with high abundance and wide distribution in the ocean, and is suitable for the rapid and accurate quantification of picocyanobacteria in different media (seawater, surface sediments, columnar sediments, etc.) of the marine environment. The invention is the first to use molecular biological methods (qPCR) to quantify the total abundance of various groups of marine picocyanobacteria, so it has broad application prospects.

The utility model relates to an encrusting machine. A rotary cutting device of the encrusting machine is provided with a forming concave cavity and a rotary cutting assembly; a discharging port of the crust raw material pushing mechanism is communicated with the crust feeding port, a discharging port of the stuffing feeding mechanism is communicated with the stuffing feeding port, the encrusting discharging port faces the forming concave cavity of the rotary cutting device, and the rotary cutting assembly cuts off raw materials below the encrusting discharging port. The total amount of wrappers and fillings is quantitatively accommodated through the forming cavity, and the rotary cutting assembly accurately cuts off the wrappers and fillings, so that formed cakes are uniform and identical in size and uniform in shape. The material extruding part is arranged to be in an inverted frustum shape, when the material extruding part descends to extrude the stuffing, the outer side surface of the material extruding part is attached to the inner container at the lowermost end of the funnel, in this way, it can be guaranteed that the amount of the material extruded every time is equal to the size of the extruding part, quantification is more accurate, and the stuffing does not continue to be conveyed downwards in the process; and the inner stuffing is prevented from being mixed into the outer skin material, so that the wrapping effect is better.

This disclosure relates to the field of electrochemical detection technology, specifically a microfluidic electrochemical detection device, comprising a base, a channel plate, a filter device, a glass cover plate, and an electrode plate. The base includes an electrode groove. The channel plate is disposed on the base and includes an inlet channel, a reaction chamber, and an outlet channel that are interconnected. The reaction chamber has a first open end and a second open end. The inlet channel includes a first flow channel and a second flow channel, with the second flow channel communicating with the reaction chamber. The outlet channel has a drain port at its end near the reaction chamber. Along the axial direction of the outlet channel, and in the direction from the side away from the reaction chamber to the side near the reaction chamber, the cross-sectional area of ​​the drain port gradually increases. The filter device is embedded within the channel plate and located between the first and second flow channels. The glass cover plate is sealed to the first open end. The electrode plate is embedded within the electrode groove and sealed to the second open end. The detection device of this disclosure exhibits high detection accuracy and provides highly accurate detection results.

The present application provides a method and kit for detecting N6-methyladenine in a nucleic acid molecule.

The present invention discloses a multi-index detection chip, which includes: a sample processing unit; a liquid storage unit, the liquid storage unit includes a connected diluent storage area and a diluent quantitative area, wherein the diluent quantitative area includes a buffer sub-area, a quantitative sub-area and a waste liquid sub-area that are interconnected, and a buffer enters the quantitative sub-area from the diluent storage area through the buffer sub-area for quantitative measurement, and the excess buffer enters the waste liquid sub-area; and a reaction unit, the reaction unit is connected to the sample processing unit and the quantitative sub-area, wherein the sample processing unit, the liquid storage unit and the reaction unit are connected to the atmosphere. By integrating sample processing, quantitative dilution of buffer and reaction of diluted samples, the operation process is simplified, and the diluent quantitative area is divided into a buffer sub-area, a quantitative sub-area and a waste liquid sub-area that are interconnected, so as to achieve accurate quantitative measurement of the diluent, and the detection efficiency and accuracy are high.

The utility model discloses a quantitative atomizing and oiling device, which relates to the technical field of atomizing and oiling devices and comprises a quantitative pipe, an atomizing head arranged on the quantitative pipe, a liquid inlet arranged on the quantitative pipe, an oil inlet pipe arranged on the liquid inlet, and a limiting plate arranged in the quantitative pipe through a first sliding rod and a threaded rod. A first bevel gear is further arranged in the quantitative tube through a mounting shaft, a second bevel gear is arranged at the tail end of the threaded rod, a worm gear is further arranged on the mounting shaft through key connection, a worm is further rotationally arranged on the quantitative tube, and the worm is meshed with the worm gear. Under the matching action of the threaded rod, the limiting plate, the mounting shaft, the first bevel gear, the second bevel gear, the worm gear and the worm, accurate control over the oil quantity in the quantitative pipe is achieved, the size of an inner cavity of the quantitative pipe is changed, quantitative adjustment over the oil quantity is achieved, the device is easy and convenient to operate, quantification is accurate, and the working efficiency is improved. And the efficiency and the precision of oil coating operation are greatly improved.