24results about How to "Quantitatively accurate" patented technology

The application belongs to the technical field of detection, and particularly relates to a method for determining the content of chlorhexidine acetate in a disinfectant. The method for determining the content of chlorhexidine acetate in the disinfectant comprises the following steps: (1) dispersing the sample with glacial acetic acid, releasing chlorhexidine acetate combined with sample matrix components, and extracting with a methanol aqueous solution; and (2) taking a tetrabutylammonium bromide-acetic acid-acetonitrile solution as a mobile phase, and detecting with a high-performance liquid chromatograph. The method for detecting chlorhexidine acetate is simple in operation, good in reproducibility, complete in separation from impurities, high in accuracy, and has a good application prospect.

The utility model discloses a quantitative strain packaging connector which comprises a stocker used for storing quantitative strains; the water storage device is used for storing quantitative clear water; the connector is used for connecting the material storage device and the water storage device; a connecting cavity is formed in the connector, the water storage device is located above the material storage device, and after the water storage device moves relative to the connector, the water storage device is communicated with the connecting cavity, so that clear water in the water storage device flows into the material storage device; the device has the advantages of being compact in structure, accurate in quantification, convenient to operate and excellent in sealing performance.

This invention discloses a wide dynamic range substrate reagent, belonging to the field of biological detection reagent technology. The reagent consists of the following components: citric acid, 9.6 g / L; 3,3',5,5'-tetramethylbenzidine, 0.29 g / L; 3% hydrogen peroxide solution, 3 mL / L; 1 g / L polyvinyl alcohol; 0.5 g / L copovidone W-735; 0.5 g / L dimethyldiallylammonium chloride-acrylamide copolymer; 1 g / L PVP K30; and Tween 20, 0.005 g / L. mL / L; polyethylene glycol 10g-50g / L; pure water, balance. This invention, by adding specific polyethylene glycol and synergistically combining with other components, significantly reduces the reagent background signal and improves detection sensitivity; effectively broadens the detection dynamic range and increases the detection upper limit of high-concentration analytes; improves the linear correlation between signal and concentration; and enhances the thermally accelerated stability of the reagent. This reagent has comprehensive performance and is especially suitable for ELISA detection systems with high sensitivity and wide detection range.

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The invention discloses a headspace-gas chromatography detection method for 1, 3-butadiene in an automotive interior material, which comprises the following steps: 1) crushing a sample into fine particles, freezing with liquid nitrogen, embrittling, and crushing at low temperature; and (2) weighing a sample which is recovered to room temperature after being crushed, putting the sample into a headspace bottle, quickly sealing, and carrying out gas chromatography detection in a headspace sampling mode. On the basis of the headspace-gas chromatography technology, by optimizing headspace sampling conditions, instrument parameters and chromatographic column selection, the 1, 3-butadiene detection method which is simple and convenient in pretreatment, high in sensitivity and accurate in quantification is established. And the method is precisely positioned on a specific and complex substrate, namely an automotive interior material. Compared with the prior art, the method is simple and convenient to operate and high in detection speed, can effectively avoid the problems of incomplete dissolution, difficulty in sample introduction and the like, is suitable for measuring an actual sample, and provides a reliable technical means for detecting the residual quantity of 1, 3-butadiene in the automotive interior material.

This invention provides a method for detecting tetrachloronaphthalene, a persistent organic pollutant in eel products. The specific steps include: eel sample preparation, target analyte extraction and matrix purification, followed by analysis using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (UPLC-APCI-MS / MS). The measured peak area, fractionation ratio, and other relevant parameters are substituted into a specific formula to calculate the measured value of tetrachloronaphthalene in the test solution. This method fills the gap in my country's technology for detecting tetrachloronaphthalene-like persistent organic pollutants in eel products. It effectively eliminates interference from the complex matrix of eel products, accurately determines four tetrachloronaphthalene isomers in trace amounts, and possesses significant advantages such as high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

The invention provides a multi-sensing module combined hydrogen detection method and system under a room temperature condition, and relates to the technical field of hydrogen sensors, and the method comprises the following steps: obtaining output signals of a first sensing module and a second sensing module in real time; when the signal value output by the first sensing module is higher than a preset safety value, a first alarm is given; when the response rate of the output signal of the first sensing module is higher than the preset response rate value, sealing of the second sensing module is relieved, and a second alarm is given out according to the output signal value of the second sensing module. According to the invention, seamless and graded monitoring of hydrogen from low concentration to ultrahigh concentration can be realized, room temperature stable induction during low-concentration hydrogen leakage can be ensured, and accurate quantification can be carried out during high-concentration hydrogen leakage; the high-value sensing module is always in a sealed state to effectively protect the value, the low-value sensing module adopts indium doped molybdenum oxide nanobelts to ensure long-time stable use at room temperature, and the two sensing modules are combined and complemented, so that the hydrogen detection safety is higher.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

This utility model discloses a wastewater treatment agent quantitative dosing device and a wastewater tank, comprising a multi-axis displacement unit, a quantitative dosing unit connected to the moving end of the multi-axis displacement unit, and a control unit. The quantitative dosing unit includes a storage component, a dosing pump, a quantitative component, and a spraying component interconnected by pipelines. The quantitative component includes a quantitative tank and a float plate disposed within the quantitative tank. The float plate divides the quantitative tank into an upper chamber and a lower chamber. The lower chamber connects to the dosing pump and the spraying component. A fixed sensor and a loading component corresponding to the sensor are disposed in the upper chamber. The loading component is connected to the float plate. The control unit is electrically connected to the sensor, the dosing pump, and the spraying component. This utility model improves the efficiency and quality of wastewater treatment and reduces the risks and costs of manual operation.

The utility model relates to the technical field of coffee machines, in particular to a coffee machine with a weighing quantitative control module, which comprises a coffee machine body, the weighing quantitative control module is arranged on the coffee machine body, and the weighing quantitative control module comprises an independent support, a weighing platform, a pressure sensor and a main control circuit board. The pressure sensor is electrically connected with the main control circuit board, the weighing platform and the pressure sensor are installed at the upper end and the lower end of the independent support respectively, and a waste water disc assembly is arranged on the weighing platform. According to the utility model, the weighing platform and the pressure sensor are fixed on the independent support, and the waste water disc assembly is adjusted to be only contacted with the weighing platform instead of being contacted with the base instead of being normally contacted with the base, so that the weighing effect can be achieved no matter whether the waste water disc assembly is taken away or not; and the whole machine is controlled by the main control circuit board to finish extraction, so that accurate and quantitative control is achieved, and the manufacturing cost is lower.

The invention relates to the technical field of treatment drug monitoring, in particular to a method for simultaneously determining the concentration of multiple antiepileptic drugs in plasma. Comprising the following steps: S1, preparing a standard working solution; s2, preparing an internal standard solution; s3, mixing the standard working solution with blank plasma, adding an internal standard solution, centrifuging, and detecting to obtain a chromatogram of a standard solution; s4, based on the chromatogram, taking the ratio of the peak area of the anti-epileptic drug to the peak area of the corresponding internal standard substance as a vertical coordinate Y, taking the ratio of the concentration of the anti-epileptic drug to the concentration of the corresponding internal standard substance as a horizontal coordinate X, and fitting to obtain a standard curve equation; s5, preparing a plurality of groups of quality control working solutions, and verifying the standard curve equation obtained in the step S4; and S6, uniformly mixing the blood to be detected with the internal standard solution, centrifuging, detecting the supernate, and calculating the concentration of the antiepileptic drug in the plasma. The method can realize simultaneous, accurate and quantitative detection of multiple antiepileptic drugs in blood.

The invention relates to a dielectric wetting valve micro-fluidic chip for metabolic marker detection and a preparation method of the dielectric wetting valve micro-fluidic chip. A chip middle layer is stacked above a chip lower layer, and an inverted chip upper layer is stacked above the chip middle layer; the chip middle layer comprises a double-sided adhesive layer and a trident micro-channel, the periphery of the trident micro-channel is coated with the double-sided adhesive layer, the trident micro-channel comprises a central area, a first branch channel, a second branch channel and a third branch channel, and the first branch channel, the second branch channel and the third branch channel are uniformly distributed on the periphery of the central area; the head end of the first branch channel, the head end of the second branch channel and the head end of the third branch channel are respectively connected with the central area. Through the trident micro-channel, the detection of various metabolic markers becomes simpler, more convenient and more sensitive, and the detection precision of the metabolic markers is improved. According to the invention, through the trifurcate micro-channel, the sequential analysis of various targeted metabolites can be realized, an external pump is not needed, and the device has the advantages of high portability, accurate fluid control and the like.

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

This invention discloses an antioxidant low-density lipoprotein monoclonal antibody and its applications, belonging to the field of antibody technology. The antioxidant low-density lipoprotein monoclonal antibody of this invention possesses high specificity, high affinity, and high sensitivity. An immunoturbidimetric assay kit prepared based on the antioxidant low-density lipoprotein monoclonal antibody of this invention can quantitatively detect oxidized low-density lipoprotein in peripheral blood samples, and can have a good diagnostic effect on cardiovascular diseases caused by atherosclerosis (AS).

The utility model provides a kind of oil dropping device for high-power oil lens microscopic imaging, including support adjustment module, oil storage module and oil inlet module, and oil inlet module and oil storage module are fixedly installed on support adjustment module;Oil inlet module includes quantitative pump, oil pipe, oil dropping needle and oil dropping needle fixer, and quantitative pump is fixedly installed on the fixed plate that support adjustment module has;Oil dropping needle is fixedly installed on the adjusting support that support adjustment module has by oil dropping needle fixer, and adjusting support is used to adjust the position of oil dropping needle, and oil dropping needle, quantitative pump and oil storage module are sequentially connected by oil pipe.This utility model ensures the quantity of each oil drop consistent by quantitative pump assembly, can realize accurate quantitative oil drop, avoids the problem that manual operation oil drop quantity is not accurate, and through support adjustment module, the position of oil dropping needle can be flexibly adjusted, and the position of oil dropping needle can be adjusted according to actual needs, to adapt to the observation needs of different samples.

The utility model relates to a kind of dry-mixed mortar automatic quantitative packing packing device, including rack, hopper and support plate, hopper is installed at the top of rack, support plate is set on rack by elastic support structure, the bottom of hopper is separately provided with solenoid valve and mechanical valve, rack is provided with instantaneous skewing mechanism, instantaneous skewing mechanism and mechanical valve are mutually matched, and instantaneous skewing mechanism is connected with elastic support structure by transmission structure, rack is horizontally symmetrical between hopper and support plate and is provided with infrared emitter and infrared receiver, the utility model, by setting elastic support structure below support plate, and it is connected with instantaneous skewing mechanism and mechanical valve, the accurate control of mechanical valve is realized, infrared emitter and infrared receiver are set between hopper and support plate of device, realize the opening and closing of solenoid valve, prevent the leakage of dry-mixed mortar when replacing container, reduce the pollution to working environment, improve production efficiency and product quality.

The utility model provides a kind of laboratory liquid quantitative measurement and canning device, comprising: intercommunication quantitative component and switch component;Wherein, the quantitative component, comprising, hollow cylindrical can body (1), the can body (1) bottom is connected with the quantitative component top;Variable capacity adjusting rod (2) is equipped in the can body (1), for adjusting the hollow cavity volume of the can body (1);The switch component, comprising, shell (8), the shell (8) top is connected with the can body (1) bottom;Switch device is equipped in the shell (8), the hollow cavity of the switch device top is communicated with the can body (1), bottom is worn out the shell (8) bottom, for discharging the liquid inside the hollow cavity, the switch device side is worn out the lateral wall of the shell (8), for transporting liquid to the inside of the hollow cavity.

This disclosure relates to the field of electrochemical detection technology, specifically a microfluidic electrochemical detection device, comprising a base, a channel plate, a filter device, a glass cover plate, and an electrode plate. The base includes an electrode groove. The channel plate is disposed on the base and includes an inlet channel, a reaction chamber, and an outlet channel that are interconnected. The reaction chamber has a first open end and a second open end. The inlet channel includes a first flow channel and a second flow channel, with the second flow channel communicating with the reaction chamber. The outlet channel has a drain port at its end near the reaction chamber. Along the axial direction of the outlet channel, and in the direction from the side away from the reaction chamber to the side near the reaction chamber, the cross-sectional area of ​​the drain port gradually increases. The filter device is embedded within the channel plate and located between the first and second flow channels. The glass cover plate is sealed to the first open end. The electrode plate is embedded within the electrode groove and sealed to the second open end. The detection device of this disclosure exhibits high detection accuracy and provides highly accurate detection results.

The utility model discloses a quantitative atomizing and oiling device, which relates to the technical field of atomizing and oiling devices and comprises a quantitative pipe, an atomizing head arranged on the quantitative pipe, a liquid inlet arranged on the quantitative pipe, an oil inlet pipe arranged on the liquid inlet, and a limiting plate arranged in the quantitative pipe through a first sliding rod and a threaded rod. A first bevel gear is further arranged in the quantitative tube through a mounting shaft, a second bevel gear is arranged at the tail end of the threaded rod, a worm gear is further arranged on the mounting shaft through key connection, a worm is further rotationally arranged on the quantitative tube, and the worm is meshed with the worm gear. Under the matching action of the threaded rod, the limiting plate, the mounting shaft, the first bevel gear, the second bevel gear, the worm gear and the worm, accurate control over the oil quantity in the quantitative pipe is achieved, the size of an inner cavity of the quantitative pipe is changed, quantitative adjustment over the oil quantity is achieved, the device is easy and convenient to operate, quantification is accurate, and the working efficiency is improved. And the efficiency and the precision of oil coating operation are greatly improved.

The utility model discloses a high frequency small flow electronic nozzle, including nozzle main part subassembly and electromagnetic pulse component, the nozzle main part subassembly includes nozzle casing, nozzle end cover, control water tray and nozzle element, the electromagnetic pulse component includes electromagnetic casing, electromagnetic pulser and electromagnetic telescopic structure, the other end fixed connection of electromagnetic casing with nozzle casing, this electromagnetic casing and nozzle casing are all inside liquid inflow channel equipped, the electromagnetic pulser sets up in the inner chamber of electromagnetic casing, the electromagnetic telescopic structure includes telescopic guide seat, telescopic link and telescopic return spring, telescopic guide seat sets up in the inner chamber of nozzle casing, telescopic link can slip through the inner chamber of telescopic guide seat, and one end of this telescopic link is equipped with the water pressure hammer for pressing the liquid to the water outlet hole of control water tray in the working condition, one end of this telescopic link is equipped with telescopic magnetic force seat. This electronic nozzle has simple structure, reliable performance, quantitative accurate and so on advantages.

This invention relates to a multivariate near-infrared quantitative model based on a one-measure-multiple-evaluation method, its construction method, and its application. The construction method includes the following steps: (1) preparing a representative sample set; (2) using chlorogenic acid as an internal reference, obtaining the content of target polyphenolic compounds in each sample in the representative sample set of step (1) using a one-measure-multiple-evaluation method based on liquid chromatography, and collecting near-infrared diffuse reflectance spectral data of each sample in the representative sample set of step (1); (3) correlating the content of target polyphenolic compounds in each sample in the representative sample set of step (2) with the near-infrared diffuse reflectance spectral data, establishing a regression model and solving it, determining the optimal number of principal components, completing the model construction, and performing model validation. The multivariate near-infrared quantitative model provided by this invention can realize the simultaneous and rapid detection of multiple target polyphenolic compounds in tobacco or its products, adapting to the needs of precise laboratory analysis and large-scale industrial screening.

The invention provides a method for determining the concentration of ibrutinib in a plasma sample. According to the method, a precipitated protein pretreatment method is preferably adopted, ibrutinib-d5 is taken as an internal standard, a Venusil MP C18 (2) chromatographic column is adopted as a chromatographic condition, an aqueous solution containing 0.2% of formic acid and 5 mM of ammonium acetate and acetonitrile containing 0.2% of formic acid are taken as mobile phases for isocratic elution, and electrospray ionization source tandem mass spectrometry is adopted for detection. Compared with the prior art, the method has the characteristic of simple and convenient pretreatment operation, the extracted sample is easy to store, the analysis speed is high, and the method is suitable for large-scale clinical research sample analysis; the method has higher sensitivity, can meet the application of ibrutinib with a smaller dosage, and is particularly suitable for detecting ibrutinib in a hemolysis sample.

The application relates to a primer and a locked nucleic acid probe group for real-time fluorescent quantitative PCR detection of two strong and weak strains of goose parvovirus, wherein the strong and weak strains of goose parvovirus include MDGPV strong and weak viruses and SBDSV strong and weak viruses, and the sequences of the primer and the locked nucleic acid probe group are shown in SEQ ID NO. 1-8. The LNA-TaqMan real-time fluorescent quantitative PCR detection method established by using the primer and the LNA-TaqMan probe group can simultaneously detect, differentially diagnose and accurately quantify the strong and weak strains of goose parvovirus (duck small goose plague virus and / or duck short-beak dwarf syndrome virus strong and weak viruses), and is high in sensitivity, strong in specificity and good in repeatability.

The invention discloses a buried gas pipeline joint nondestructive testing technology, and belongs to the technical field of pipeline nondestructive testing, the buried gas pipeline joint nondestructive testing technology comprises four core steps of preparation before detection, ultrasonic phased array detection, X-ray detection, combination determination and result output, and by customizing a test block calibration device, detection parameters are optimized according to pipeline specifications, and the pipeline joint nondestructive testing efficiency is improved. An ultrasonic phased array method and an X-ray method are combined for detection, and a standardized judgment rule is established; and meanwhile, the matched special auxiliary device comprises an ultrasonic phased array detection auxiliary device and an X-ray machine positioning device which are respectively used for fixing the probe and the X-ray machine, so that accurate detection positioning and tight probe fitting are ensured. According to the method, the defect recognition rate is larger than or equal to 98%, the defect position error is smaller than or equal to + / -2 mm, the method is matched with De63-De355 full-specification PE pipeline joints, operation is convenient and fast, efficiency is high, various common defects of the joints can be comprehensively recognized, quantitative analysis and qualification judgment are achieved, and reliable technical guarantee is provided for buried gas pipeline joint quality control and safe operation.