10results about How to "Quantitatively accurate" patented technology

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

This utility model discloses a wastewater treatment agent quantitative dosing device and a wastewater tank, comprising a multi-axis displacement unit, a quantitative dosing unit connected to the moving end of the multi-axis displacement unit, and a control unit. The quantitative dosing unit includes a storage component, a dosing pump, a quantitative component, and a spraying component interconnected by pipelines. The quantitative component includes a quantitative tank and a float plate disposed within the quantitative tank. The float plate divides the quantitative tank into an upper chamber and a lower chamber. The lower chamber connects to the dosing pump and the spraying component. A fixed sensor and a loading component corresponding to the sensor are disposed in the upper chamber. The loading component is connected to the float plate. The control unit is electrically connected to the sensor, the dosing pump, and the spraying component. This utility model improves the efficiency and quality of wastewater treatment and reduces the risks and costs of manual operation.

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

This invention discloses an antioxidant low-density lipoprotein monoclonal antibody and its applications, belonging to the field of antibody technology. The antioxidant low-density lipoprotein monoclonal antibody of this invention possesses high specificity, high affinity, and high sensitivity. An immunoturbidimetric assay kit prepared based on the antioxidant low-density lipoprotein monoclonal antibody of this invention can quantitatively detect oxidized low-density lipoprotein in peripheral blood samples, and can have a good diagnostic effect on cardiovascular diseases caused by atherosclerosis (AS).

The utility model provides a kind of oil dropping device for high-power oil lens microscopic imaging, including support adjustment module, oil storage module and oil inlet module, and oil inlet module and oil storage module are fixedly installed on support adjustment module;Oil inlet module includes quantitative pump, oil pipe, oil dropping needle and oil dropping needle fixer, and quantitative pump is fixedly installed on the fixed plate that support adjustment module has;Oil dropping needle is fixedly installed on the adjusting support that support adjustment module has by oil dropping needle fixer, and adjusting support is used to adjust the position of oil dropping needle, and oil dropping needle, quantitative pump and oil storage module are sequentially connected by oil pipe.This utility model ensures the quantity of each oil drop consistent by quantitative pump assembly, can realize accurate quantitative oil drop, avoids the problem that manual operation oil drop quantity is not accurate, and through support adjustment module, the position of oil dropping needle can be flexibly adjusted, and the position of oil dropping needle can be adjusted according to actual needs, to adapt to the observation needs of different samples.

The utility model relates to a kind of dry-mixed mortar automatic quantitative packing packing device, including rack, hopper and support plate, hopper is installed at the top of rack, support plate is set on rack by elastic support structure, the bottom of hopper is separately provided with solenoid valve and mechanical valve, rack is provided with instantaneous skewing mechanism, instantaneous skewing mechanism and mechanical valve are mutually matched, and instantaneous skewing mechanism is connected with elastic support structure by transmission structure, rack is horizontally symmetrical between hopper and support plate and is provided with infrared emitter and infrared receiver, the utility model, by setting elastic support structure below support plate, and it is connected with instantaneous skewing mechanism and mechanical valve, the accurate control of mechanical valve is realized, infrared emitter and infrared receiver are set between hopper and support plate of device, realize the opening and closing of solenoid valve, prevent the leakage of dry-mixed mortar when replacing container, reduce the pollution to working environment, improve production efficiency and product quality.

This disclosure relates to the field of electrochemical detection technology, specifically a microfluidic electrochemical detection device, comprising a base, a channel plate, a filter device, a glass cover plate, and an electrode plate. The base includes an electrode groove. The channel plate is disposed on the base and includes an inlet channel, a reaction chamber, and an outlet channel that are interconnected. The reaction chamber has a first open end and a second open end. The inlet channel includes a first flow channel and a second flow channel, with the second flow channel communicating with the reaction chamber. The outlet channel has a drain port at its end near the reaction chamber. Along the axial direction of the outlet channel, and in the direction from the side away from the reaction chamber to the side near the reaction chamber, the cross-sectional area of ​​the drain port gradually increases. The filter device is embedded within the channel plate and located between the first and second flow channels. The glass cover plate is sealed to the first open end. The electrode plate is embedded within the electrode groove and sealed to the second open end. The detection device of this disclosure exhibits high detection accuracy and provides highly accurate detection results.

This invention relates to a multivariate near-infrared quantitative model based on a one-measure-multiple-evaluation method, its construction method, and its application. The construction method includes the following steps: (1) preparing a representative sample set; (2) using chlorogenic acid as an internal reference, obtaining the content of target polyphenolic compounds in each sample in the representative sample set of step (1) using a one-measure-multiple-evaluation method based on liquid chromatography, and collecting near-infrared diffuse reflectance spectral data of each sample in the representative sample set of step (1); (3) correlating the content of target polyphenolic compounds in each sample in the representative sample set of step (2) with the near-infrared diffuse reflectance spectral data, establishing a regression model and solving it, determining the optimal number of principal components, completing the model construction, and performing model validation. The multivariate near-infrared quantitative model provided by this invention can realize the simultaneous and rapid detection of multiple target polyphenolic compounds in tobacco or its products, adapting to the needs of precise laboratory analysis and large-scale industrial screening.

The application relates to a primer and a locked nucleic acid probe group for real-time fluorescent quantitative PCR detection of two strong and weak strains of goose parvovirus, wherein the strong and weak strains of goose parvovirus include MDGPV strong and weak viruses and SBDSV strong and weak viruses, and the sequences of the primer and the locked nucleic acid probe group are shown in SEQ ID NO. 1-8. The LNA-TaqMan real-time fluorescent quantitative PCR detection method established by using the primer and the LNA-TaqMan probe group can simultaneously detect, differentially diagnose and accurately quantify the strong and weak strains of goose parvovirus (duck small goose plague virus and / or duck short-beak dwarf syndrome virus strong and weak viruses), and is high in sensitivity, strong in specificity and good in repeatability.