Chiral analysis method for nicotine in tobacco juice of electronic cigarette

An electronic cigarette smoke liquid and analysis method technology, applied in the field of tobacco chemical composition analysis, can solve problems such as no nicotine chirality analysis method, and achieve the effects of low detection limit, accurate quantification and good reproducibility

Inactive Publication Date: 2015-01-21
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
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Problems solved by technology

[0005] At present, there is no report on the chiral analysis method of nicotine in e-cigarette liquid

Method used

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  • Chiral analysis method for nicotine in tobacco juice of electronic cigarette

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Embodiment Construction

[0018] The gas chromatography-mass spectrometry analytical method that applies the present invention to middle nicotine chiral analysis is further explained:

[0019] Instruments and Reagents

[0020] Agilent 7890 gas chromatography-5975 mass spectrometer; CP224S analytical balance (sensitivity 0.0001g); ultrasonic oscillator; S-(-)-nicotine, racemic nicotine (S-(-)-nicotine and R-(+)-nicotine 50% mixture) standard; methanol, methyl tert-butyl ether (chromatographically pure); chiral stationary phase capillary column: DB-cyclodex B, the specification is 60 m × 0.25 mm i.d. × 0.25 μm d.f.

[0021] Preparation of standard solution :

[0022] Weigh about 50.0 mg of racemic nicotine standard substance, dilute it with methyl tert-butyl ether to a 10 mL brown volumetric flask, and prepare a standard stock solution with a concentration of about 5 mg / mL; take the standard stock solution Dilute and prepare a 100 μg / mL racemic nicotine standard solution with methyl tert-butyl eth...

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Abstract

The invention discloses a chiral analysis method for nicotine in tobacco juice of an electronic cigarette. The chiral analysis method comprises the steps of diluting the tobacco juice of the electronic cigarette by a methyl alcohol/methyl tertiary-butyl ether solution, analyzing S-(-)-nicotine and R-(+)-nicotine in the tobacco juice of the electronic cigarette by using a chiral stationary phase capillary column mthrough a gas chromatograph-mass spectrometer, performing chiral analysis on the S-(-)-nicotine and the R-(+)-nicotine by comparing the retention time of a chromatographic peak and characteristic ions of nicotine in a standard sample with the retention time of a chromatographic peak and characteristic ions of nicotine in an electronic cigarette tobacco juice sample, and normalizing areas of quantitative ion peaks of the S-(-)-nicotine and the R-(+)-nicotine to quantify the proportions of the S-(-)-nicotine and the R-(+)-nicotine to the total nicotine. The chiral analysis method has the advantages that the nicotine in the tobacco juice of the electronic cigarette is separated by a chiral stationary phase capillary column, so that the S-(-)-nicotine and the R-(+)-nicotine in the nicotine can be better separated, and accurate and quantitative analysis on the nicotine can be realized. The sample treatment is simple and convenient; the chiral analysis method is low in detection limit, high in sensitivity, high in reproducibility, accurate in quantification and suitable for chiral analysis on the nicotine in a large batch of electronic cigarette tobacco juice samples.

Description

technical field [0001] The invention relates to a chiral analysis method for nicotine in electronic cigarette liquid, and belongs to the field of tobacco chemical component analysis. Background technique [0002] Nicotine, also known as nicotine, is the most abundant alkaloid in tobacco and smoke. It can directly stimulate the central nervous system of the human body and produce physiological reactions. It is the main driving force for smokers to smoke. Nicotine contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring. Therefore, nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. Optical enantiomers usually have completely different metabolic mechanisms and physiological properties. The chiral analysis of nicotine is of great significance in pharmacy and smoking and health. The results of in vitro cell and animal experiments show that the physiological activity of S-(-)-nicotine is stronger than that of R-(+)-nicotine. Toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N3/06
Inventor 蔡君兰赵阁余晶晶陈黎王冰崔华鹏郭军伟刘绍锋赵乐张晓兵刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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