1326 results about "Alkaloid" patented technology

The invention relates to a denitration catalyst capable of resisting alkali metal, water and sulfur composite poisoning and a preparation method and application thereof, and belongs to the field of environment-friendly catalytic materials and air pollution control. An organic solvent is used as a template agent, cerium oxide, manganese oxide and tungsten oxide are used as active components, and a cerium-manganese-tungsten composite oxide active component with a porous structure and strong acidity is prepared by a coprecipitation-soft template method. The catalyst has a wide denitration temperature window, high denitration efficiency and excellent composite poisoning resistance stability. The catalyst component is environment-friendly, low in cost and simple in preparation method, and is particularly suitable for stable and efficient purification of NOx in complex flue gas in the cement industry.

The invention relates to the technical field of environmental remediation, and discloses a biochemical synergistic remediation method for heavy metal contaminated soil, which comprises the following steps: S1, sampling the heavy metal contaminated soil, and analyzing the type, concentration and occurrence form of heavy metals of the heavy metal contaminated soil; s2, extracting active ingredients of polyphenols, organic acids or alkaloids from the plant materials; s3, applying a plant source activating agent to regulate the chemical form of the heavy metal in the soil and promote the activation of the heavy metal; s4, inoculating a functional microbial agent to enhance the heavy metal immobilization, transformation and phytoremediation capabilities; s5, planting hyperaccumulators, and cooperatively using the plant extract, the plant source activator and the microbial agent; s6, plant residue treatment and soil improvement. Through the synergistic effect of the plant extract, the plant source activator, the microbial agent and the hyperaccumulator, the plant source activator, the microbial agent and the hyperaccumulator can jointly act on the heavy metal contaminated soil from multiple ways, and chelating, activating, degrading and fixing of heavy metal are rapidly achieved.

The invention discloses a rhizoma menispermi medicinal material and formula granule UPLC (Ultra Performance Liquid Chromatography) identification method, which is characterized in that specified values of relative retention time of the rhizoma menispermi medicinal material and formula granule are determined through a high performance liquid chromatography, and the relative retention time of a corresponding test solution is respectively within + / -10% of the corresponding specified values of the rhizoma menispermi medicinal material and formula granule. The method can effectively identify the rhizoma menispermi medicinal material and the formula granules, can be used for identifying rhizoma menispermi and counterfeit berba aristolochiae mollissimae and subprostrate sophora medicinal materials and formula granules, and can also be used for identifying rhizoma coptidis, cortex phellodendri, caulis sinomenii and other medicinal materials containing alkaloid components and formula granules. The method overcomes the defects of the existing method, and has the characteristics of simplicity in operation, good stability, rich chromatographic peak information amount, good repeatability and high stability.

The invention discloses a method for extracting active ingredients in pollen typhae based on a natural eutectic solvent, and belongs to the technical field of active substance extraction. By screening the natural eutectic solvent and optimizing preparation parameters, the invention provides the method for extracting the active components in the pollen typhae by using the natural eutectic solvent, and solves the problems of low extraction rate of the active components in the pollen typhae and the like in the prior art. According to the method, proline-lactic acid (the molar ratio is 1: 3) is used as a natural eutectic solvent, and the extraction efficiency of total active substances (polyphenol, flavone and alkaloid) in the pollen typhae reaches 167.9 mg / g of pollen typhae powder; the extraction of quercetin reaches 0.41 mg / g of pollen typhae powder, and the extraction effect is far better than that of conventional water extraction and alcohol extraction.

The invention discloses a preparation method of four new skeleton alkaloid compounds with anti-inflammatory effect in the root of Anachymin and the use of the anti-inflammatory activity thereof. The four new skeleton alkaloid compounds are extracted from the root (root of Anachymin) of Roman pyrethrum (Anacyclus pyrethrum (L.) DC.) by using an organic solvent, and then separated by two or three methods of normal phase silica gel column chromatography, reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain eight new skeleton alkaloid monomer compounds, and the structures thereof are identified by high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy and X-ray single crystal diffraction. The invention also provides the NO inhibition effect of these compounds.

The present disclosure relates to tobacco plants, plant parts, seeds, compositions and methods related to modulating expression of novel genes in tobacco to modulate alkaloid levels.

The invention relates to the technical field of ferula asafoetida endophytic fungi separation and purification, in particular to alkaloid compounds in ferula asafoetida endophytic fungi as well as a preparation method and application of the alkaloid compounds in the ferula asafoetida endophytic fungi. And extracting with dichloromethane to obtain dichloromethane part extract, and purifying and separating the dichloromethane part extract by adopting silica gel column chromatography and high performance liquid chromatography to obtain the alkaloid compounds in the endophytic fungi of ferula sinkiangensis. The alkaloid compound in the ferula sinkiangensis endophytic fungi is disclosed for the first time, the compound is subjected to an in-vitro anti-tumor pharmacodynamic experiment, and according to the experiment result, the alkaloid compound has a certain inhibition effect on MCF-7 cells; therefore, the alkaloid compound can be used for preparing the medicine for preventing and treating the breast cancer, and a new medicine is provided for preventing and treating the breast cancer.

The invention discloses a temperature-sensitive hydrogel based on Chinese prickly ash alkaloid. The temperature-sensitive hydrogel is prepared from poloxamer 407, poloxamer 188 and Chinese prickly ash alkaloid powder. The temperature-sensitive hydrogel based on the pepper alkaloid prepared by the invention has three characteristics of biological adhesion, self-healing property and slow release property, and solves the technical problems that the pepper alkaloid is poor in stability and uncontrollable in drug release.

The invention relates to the technical field of ferula asafetida endophytic fungus strain separation and purification, in particular to an alkaloid component in pleurotus nebrodensis as well as a preparation method and application thereof, and the alkaloid component in pleurotus nebrodensis, namely an alkaloid compound in pleurotus nebrodensis, is prepared by the following steps: crushing pleurotus nebrodensis sporocarp, adding methanol, heating, refluxing and extracting; dispersing the pleurotus nebrodensis total extract into a suspension with water, and extracting to obtain a petroleum ether part and ethyl acetate part extract; and carrying out gradient elution on the ethyl acetate part extract by silica gel column chromatography, and separating and purifying the third fraction by high performance liquid chromatography to obtain the alkaloid compounds in pleurotus nebrodensis. The invention discloses the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C for the first time, and the pleurotus nebrodensis alkali C has a certain inhibition effect on HGC-27 cells, so that the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C, can be applied to the preparation of tumor prevention drugs and antitumor drugs.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

This invention relates to the extraction of psychoactive compounds from fungus for use in medicine. Raw fungus is dried and ground. The solvent used for extraction is methanol or a hydro-methanol mixture, an acidic hydro-methanol mixture, or an alkaline hydro-methanol mixture. The extraction slurry is filtered and pH-adjusted if necessary. The methanol in the solvent is then completely evaporated and water added back, where necessary, to form a concentrated slurry. The concentrated slurry is then standardized to provide a known concentration of the psychoactive alkaloids that have been extracted. The standardized slurry may then be dried to result in a powdered extract with a precisely defined purity of psychoactive compounds.

The invention provides a marine biological alkali compound. The chemical formula of the marine biological alkali compound is as follows: # imgabs0. The marine biological alkali compound or pharmaceutically acceptable salt thereof can be used for preparing antibacterial drugs or anti-infective drugs. The invention also provides a separation and extraction preparation method or a chemical synthesis preparation method of the marine alkaloid compound or the pharmaceutically acceptable salt thereof, and the preparation method is simple and easy to industrialize. The invention also provides application of the marine biological alkali compound or the pharmaceutically acceptable salt thereof in preparation of antibacterial drugs or anti-infection drugs for human or fish.

Use of a mulberry extract in the preparation of a product for treating and / or improving polycystic ovarian syndrome. It is demonstrated in the experiment that the mulberry twig alkaloids can reduce the ovarian weight of rats with polycystic ovarian syndrome and improve the ovarian pathology, regulate the estrous cycle of rats with polycystic ovarian syndrome in the intervention process, and can remarkably adjust the secretion level of sex hormones in rats with polycystic ovarian syndrome.

The invention provides an alkaloid compound as well as a preparation method and an application thereof in tumor resistance. The alkaloid compound provided by the invention shows a remarkable cytotoxic effect on human non-small cell lung cancer cells A549, mouse lung adenocarcinoma cells Lewis, human triple negative breast cancer cells MDA-MB-231 and mouse breast cancer cells 4T1, and can be used for preparing medicines for treating breast cancer, lung cancer and the like.

An embodiment of the present invention provides a tobacco plant having a low alkaloid content. In the tobacco plant, a function of a gene encoding aspartate oxidase AO2 is suppressed.

Use of a mulberry extract in the preparation of a product for treating and / or improving polycystic ovarian syndrome. It is demonstrated in the experiment that the mulberry twig alkaloids can reduce the ovarian weight of rats with polycystic ovarian syndrome and improve the ovarian pathology, regulate the estrous cycle of rats with polycystic ovarian syndrome in the intervention process, and can remarkably adjust the secretion level of sex hormones in rats with polycystic ovarian syndrome.

The invention relates to the technical field of microbiology and food processing, in particular to pediococcus acidilactici and application of the pediococcus acidilactici in preparation of a brain-intestinal axis regulating efficacy metagen product.The high-activity pediococcus acidilactici DY-1 is separated out from natural fermentation pickles of super-polyselenium plant cardamine violifolia for the first time; the strain can be widely applied to fermentation of high-selenium plant-based food, can significantly promote glycolysis release of selenium-containing active substances, has good acid resistance, high protease activity and high lipase activity, can fully ferment plant-based fermentation raw materials, and has high yield. Glycolysis of macromolecular substances such as protein, saccharides and fat is facilitated, accumulation of active ingredients such as essential amino acid, unsaturated fatty acid, alkaloid and phenolic substances is promoted, and the edibility and functionality of the product are improved; and the organic selenium-rich metagen product prepared by sterilizing the fermentation mixture can synergistically improve human body cognition and emotion regulation regulated by microorganism-intestinal-brain axis.

The invention provides a cultivation technology for improving the alkaloid content of thunberg fritillary bulb, relates to the links of variety selection and sowing, field management and harvesting, and aims to improve the alkaloid content of the thunberg fritillary bulb and give consideration to the yield. Comprising the steps of proper variety selection and sowing, scientific field management, reasonable moisture and fertilizer management, effective intertillage weeding and pest control, timely flower picking, topping and shading, precise topdressing and timely harvesting. The Zhejiang fritillary bulb has better medicinal effects of clearing heat, eliminating phlegm, stopping coughing, detoxifying, removing stasis, eliminating carbuncle and the like, yield can be considered to a certain extent, the requirement of the Zhejiang fritillary bulb for the environment is met, the yield is kept flush with that of open-air cultivation, great reduction of the yield caused by quality improvement is avoided, and the goals of high quality and high yield are achieved. Meanwhile, cultivation is conducted under the fallen leaf forest to improve the land utilization rate, the market requirement is met, the development of the thunberg fritillary bulb industry is promoted, and therefore the win-win situation of ecological benefits and economic benefits is achieved.

The invention discloses an ultra-low nicotine tobacco variety matching cultivation method, and relates to the technical field of crop characteristic variety cultivation. Comprising the following steps that a tobacco cultivation field and a tobacco variety to be cultivated are selected, seedling raising is carried out, nitrogen is reasonably applied to the tobacco cultivation field, tobacco seedlings are transplanted to the tobacco cultivation field, topdressing is carried out on tobacco regularly, insect pest prevention is carried out in a chemical insect prevention mode, tobacco plants are topped, leaves are reserved, and harvesting is carried out when the tobacco is mature; according to the method, the low-nicotine flue-cured tobacco new strain CD01 is used as a material, the content of alkaloid and the quality of tobacco leaves can be effectively regulated and controlled by reasonably setting the nitrogen application amount, the number of left leaves and the planting density in the tobacco cultivation process, the nicotine content can be kept below 0.8%, the requirement of low-nicotine cigarettes for raw materials can be met, and therefore the economic character of the tobacco leaves is improved, and the tobacco quality is improved. The chemical components of the tobacco leaves are more coordinated, and the quality and yield of the tobacco leaves are ensured while the nicotine content of ultralow-nicotine varieties is not increased.

The invention discloses a Lactobacillus buchneri strain with high yield of gamma-aminobutyric acid and application of the Lactobacillus buchneri strain in preparation of nutritional and healthy fruit and vegetable juice. The strain is Lactobacillus buchneri GM3, and is preserved in the Guangdong Microbial Culture Collection Center on January 9, 2025, and the preservation number of the strain is GDMCC No: 65737. The invention further discloses a preparation method of the Lactobacillus buchneri strain. The strain not only has the capacity of producing gamma-aminobutyric acid at high yield, but also has good acid resistance, cholate resistance and gastrointestinal fluid resistance, and is very suitable for fermentation production of fermented fruit and vegetable juice. The contents of gamma-aminobutyric acid and flavonoid substances in fruit and vegetable juice fermented by the strain are greatly increased, and the nutritional value of the fruit juice is remarkably increased; moreover, the contents of alkaloid substances and total sugar are remarkably reduced, so that the tea is healthier, and the flavor is better and more unique. The Lactobacillus buchneri GM3 is a safe lactic acid bacterium, and the Lactobacillus buchneri GM3 and the technology for producing fruit and vegetable juice through fermentation of the Lactobacillus buchneri GM3 have very good application value and market prospect.

The invention discloses medicinal and edible mulberry leaf drink substitute tea and a processing method thereof, and belongs to the technical field of mulberry leaf tea drink production. The processing method comprises the following steps: picking mulberry leaves, rinsing with ice water, carrying out synergistic fixation with ginger juice and steam, rolling and forming, dipping with a mulberry polysaccharide-iron compound solution in combination with a low-frequency electromagnetic field, and then treating with a staged oxygen-controlled fermentation process. Wherein the ginger juice steam enzyme deactivation system accelerates enzyme deactivation through the synergistic effect of ginger volatile oil and high-temperature steam under the conditions that the temperature is 105-110 DEG C and the pressure is 0.10-0.25 MPa; the mulberry polysaccharide-iron compound permeates into mulberry leaf cells under the assistance of a low-frequency electromagnetic field, and synthesis of gamma-aminobutyric acid is promoted; in the primary fermentation stage, eurotium cristatum is utilized to carry out anaerobic metabolism under mediation of Fe < 3 + >, and in the secondary fermentation, a metabolic pathway is optimized through combination of lactobacillus plantarum and bacillus subtilis. The prepared tea substitute is rich in GABA (gamma-aminobutyric acid), flavonoid substances and alkaloid substance DNJ, has the physiological effects of reducing blood sugar and blood fat, and can relieve pancreas burden.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an inhalation type pharmaceutical composition for treating inflammatory respiratory diseases and a preparation method thereof. The inhalation type pharmaceutical composition comprises an active component and a nano-liposome, and mannose is modified on the surface of the nano-liposome; the active ingredients comprise bulbus fritillariae cirrhosae total alkaloids and platycodin D; the nano-liposome is prepared from the following raw materials: phospholipid, cholesterol and cholesterol-polyethylene glycol 1000-mannose ester. The liposome can be specifically recognized by a mannose receptor highly expressed on the surface of alveolar macrophage through a surface-modified mannose ligand, so that the receptor-mediated endocytosis is started, and the wrapped bulbus fritillariae cirrhosae total alkaloids and platycodin D are efficiently introduced into cells. The inhaled pharmaceutical composition of the present invention collectively pushes macrophages from a pro-inflammatory M1 phenotype to a repairable M2 phenotype. Therefore, the overall curative effect of the combination of the two components is far better than that of the independent use of any component.

The invention provides a matrine-natural polyphenol amorphous substance as well as a preparation method and application thereof, and relates to the technical field of polyphenol and alkaloid pharmacy, in the matrine-natural polyphenol amorphous substance, the molar ratio of matrine to natural polyphenol is (1-2): (1-2), through strong interaction between matrine and polyphenol substances, the matrine-natural polyphenol amorphous substance can be used for preparing the natural polyphenol, and the matrine-natural polyphenol amorphous substance can be used for preparing the natural polyphenol. The physicochemical properties of the medicine in the amorphous substance, such as solubility, polarity and lipid-water partition coefficient, are changed, the slow release of the medicine is realized, and the IC50 value of the amorphous substance is reduced; in addition, matrine and polyphenol substances exert synergistic anti-oxidative stress capability, the ROS (reactive oxygen species) level of cells is reduced, and the toxicity of natural polyphenols to the cells is further reduced.

The invention discloses a recombinant bacterium and application of the recombinant bacterium in production of benzophenanthridine alkaloid. The synthetic route of the benzophenanthridine alkaloid is divided into a plurality of modules for research, and de novo synthesis of the benzophenanthridine alkaloid is realized through a plurality of strategies, for example, in order to improve the activity of BBE, a new endoplasmic reticulum engineering strategy is developed, so that the yield of a key intermediate S-Soulerine is increased by 3 times; the McDBOX2 is identified as the only reported DBOX gene which plays a role in saccharomyces cerevisiae at present, and the reconstruction of the whole biosynthetic pathway of the benzophenanthridine alkaloid is completed; by carrying out N-terminal truncation operation on DBOX, cytoplasmic expression and endoplasmic reticulum amplification are improved, and supply of a cofactor FAD is increased, so that the yield of benzophenanthridine alkaloid is increased. Experiments prove that the recombinant strain constructed by the invention can be used for efficiently producing benzophenanthridine alkaloid. The method has an important application value.

The invention discloses a preparation method and application of an areca total alkali extract. The areca total alkali is calculated by arecoline, arecoline, demethylated arecoline and demethylated arecoline. The preparation method comprises the following steps: carrying out water extraction on areca catechu, purifying an extracting solution through cationic resin, concentrating an eluent, regulating acid, carrying out suction filtration, and drying to obtain the areca catechu total alkali extract. According to the preparation method, high-purity alkaloid can be obtained from areca nuts, the process is simple, convenient and stable, the cost is low, the preparation method can be widely applied to industrial production, and the preparation method has the effects of improving rumen fermentation characteristics, protecting rumen health, improving nutrient digestibility, promoting animal growth and improving animal production performance when applied to economic animals.

The invention belongs to the technical field of tea leaf component detection methods, and particularly relates to a method for simultaneously detecting 4 alkaloids and 10 catechin components in tea leaves, which utilizes high performance liquid chromatography to simultaneously detect 4 alkaloids and 10 catechin components in tea leaf samples. And obtaining the contents of the 14 characteristic components in the tea sample according to the respective standard curves of the four alkaloids and the 10 catechin components. According to the detection method provided by the invention, 14 compounds in the tea leaves can be simultaneously detected and separated, and the problem that the separation effect is poor when multiple compounds in the tea leaves are simultaneously analyzed in the prior art is solved, so that the high-standard requirement of tea leaf component analysis is met; a more efficient, accurate and reliable analysis tool is provided for tea manufacturers, detection mechanisms and scientific researchers, and powerful support is provided for the fields of tea quality control, scientific research, deep processing and the like.

Methods for treating a neuropsychiatric disorder by administering an iboga alkaloid and a cardioprotective agent in conjunction with analysis of brain image data is described. Also described are methods to improve brain health and to slow or reverse brain aging by disorder by administering an iboga alkaloid and a cardioprotective agent, where analysis of brain image data is used to monitor and / or evaluate treatment effectiveness.

The invention discloses a method for extracting active ingredients in pekoe silver needles based on a natural eutectic solvent, and belongs to the technical field of active substance extraction. According to the method for extracting the active ingredients in the pekoe needle through the natural deep-eutectic solvent, the natural deep-eutectic solvent is selected, the water content, the material-liquid ratio and the extraction conditions are optimized, and the problems that in the prior art, the extraction rate of the active ingredients of the pekoe needle is low and the like are solved. The proline glycerin (with the molar ratio of 1: 3) prepared by the invention is used as a natural eutectic solvent, and the extraction efficiency of total active substances (polyphenol, flavone and alkaloid) in the pekoe silver needle is 280mg / g of pekoe silver needle powder; the extraction rate of theanine reaches 21 mg / g of Baihao silver needle powder, and the extraction effect is far better than that of conventional water extraction and alcohol extraction.

The present invention relates to a method for preparing a highly conjugated tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid compound having a unique 8,14,18,24-tetraazaoctacyclic [21.2.21,4.1.02,21.03,18.05,17.09,16.011,15] nonacosane ring system, and the anti-inflammatory and analgesic use of the compound. With regard to the tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid compound, the root of Anacyclus pyrethrum (L.) DC is extracted using an organic solvent, the resulting extract is extracted, separation is then performed by means of two to three methods of normal-phase silica-gel column chromatography, reversed-phase silica-gel column chromatography and semi-preparative high-performance liquid chromatography to obtain four tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid monomer compounds having a new skeleton, and the structures of the compounds are identified by means of methods such as high-resolution mass spectrometry, and nuclear magnetic resonance spectroscopy. Further provided in the present invention are the Nav1.2 inhibitory effect and NO inhibitory effect of the compounds.

The invention discloses mulberry leaf flavone as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction and preparation. Flavone is released through a microwave-assisted enzyme method, flavone is dissolved through a deep eutectic solvent, the reaction rate is increased through microwave treatment, meanwhile, the catalytic activity of enzyme can be enhanced, and the enzymatic reaction speed and yield are effectively increased; the deep-eutectic solvent contains a large amount of hydroxyl groups and amino groups, the hydroxyl groups and the amino groups can easily form hydrogen bonds with flavone, the solubility of the flavone in the deep-eutectic solvent can be increased, and meanwhile the total extraction amount of the flavone is increased under the dual auxiliary effects of microwaves and enzyme; by adopting a resin separation method, high-purity mulberry leaf flavone, mulberry leaf alkaloid and mulberry leaf polysaccharide can be obtained. The mulberry leaf flavone and the mulberry leaf alkaloid which are prepared and separated by the method can be conveniently and rapidly proportioned according to the requirements, and the mulberry leaf flavone and the mulberry leaf alkaloid can be widely applied to the aspect of hypoglycemic food.