527 results about "Alkaloid" patented technology

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention provides an alkaloid compound as well as a preparation method and an application thereof in tumor resistance. The alkaloid compound provided by the invention shows a remarkable cytotoxic effect on human non-small cell lung cancer cells A549, mouse lung adenocarcinoma cells Lewis, human triple negative breast cancer cells MDA-MB-231 and mouse breast cancer cells 4T1, and can be used for preparing medicines for treating breast cancer, lung cancer and the like.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an inhalation type pharmaceutical composition for treating inflammatory respiratory diseases and a preparation method thereof. The inhalation type pharmaceutical composition comprises an active component and a nano-liposome, and mannose is modified on the surface of the nano-liposome; the active ingredients comprise bulbus fritillariae cirrhosae total alkaloids and platycodin D; the nano-liposome is prepared from the following raw materials: phospholipid, cholesterol and cholesterol-polyethylene glycol 1000-mannose ester. The liposome can be specifically recognized by a mannose receptor highly expressed on the surface of alveolar macrophage through a surface-modified mannose ligand, so that the receptor-mediated endocytosis is started, and the wrapped bulbus fritillariae cirrhosae total alkaloids and platycodin D are efficiently introduced into cells. The inhaled pharmaceutical composition of the present invention collectively pushes macrophages from a pro-inflammatory M1 phenotype to a repairable M2 phenotype. Therefore, the overall curative effect of the combination of the two components is far better than that of the independent use of any component.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses application of a cytisine type alkaloid and a derivative thereof in preparation of a medicine for preventing or treating respiratory syncytial virus infection, and relates to the technical field of respiratory syncytial virus prevention and treatment. The research finds that the natural product cytisine shows good anti-respiratory syncytial virus infection activity in vivo and in vitro, and finds that the cytisine can obviously relieve lung injury caused by virus infection and reduce lung inflammation; the invention provides an experimental basis for developing an efficient and safe drug for resisting respiratory syncytial virus (RSV) infection. As a natural product, the cytisine reduces the social burden caused by treatment of respiratory syncytial virus infection and reduces the treatment cost.

The invention relates to the technical field of dendrobium officinale processing, and particularly discloses a method for realizing high value-added conversion of dendrobium officinale alkaloid based on efficient extraction. The method for realizing high value-added conversion of dendrobium officinale alkaloid based on efficient extraction comprises the following steps: S1, pretreatment and activation of raw materials: crushing dried dendrobium officinale stalks to 60-100 meshes to obtain dendrobium powder; mixing the dendrobium nobile powder with an activating agent aqueous solution to obtain activated wet dendrobium nobile powder; s2, deep eutectic solvent synergistic microwave extraction; s3, precipitation and enrichment of an anti-solvent; s4, cleaning and drying the precipitate; s5, preparing alkaloid nanoparticles; and S6, preparing the functional health-care product preparation. As a functional dietary supplement, the dendrobium officinale alkaloid solid beverage tablet or capsule provided by the invention can be used for daily health care, and has the advantages of high bioavailability, strong intestinal synergistic regulation function and good physical stability.

The invention discloses a broad-spectrum combined medicine for treating liver cancer, which comprises purified water, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid, chamaejasmine, gelsmium elegans alkaloid, xanthoxylin and various B medicines, and further comprises folic acid, vitamins, copper sulfate, zinc sulfate, manganese sulfate, yeast and penicillin sodium. Through the multi-component, multi-target and multi-path design, the medicine disclosed by the invention shows a potential effect of inhibiting the progress of liver cancer and good safety in cell experiments, animal models and human individual case observation. Cellular immunity and humoral immunity can be enhanced, the treatment cost is lower than that of a traditional treatment scheme, and no pain is caused during treatment.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention discloses a cephalotaxus hainanensis Ch2OGD1 gene and application thereof, and belongs to the technical field of gene engineering. The Ch2OGD1 gene is used for coding a 2-oxoglutaric acid dependent dioxygenase (2OGD) type Pictet-Spengler enzyme, and the Pictet-Spengler enzyme is used for coding a Pictet-Spengler enzyme. Through a tobacco transient expression system and in-vitro enzyme activity determination, the Ch2OGD1 is proved to be capable of specifically catalyzing the condensation of dopamine and 4-hydroxyphenylpropanal to generate 1-phenethyl isoquinoline. The invention provides a directional preparation method of 1-phenethyl isoquinoline based on the Ch2OGD1 gene, provides a key enzyme element for biosynthesis of a cephalotaxine alkaloid precursor compound, and provides a technical basis for expanding the application of the gene in plant genetic engineering. And a potential technical path is provided for relieving the problem of resource shortage of cephalotaxine alkaloids.

The invention discloses an esophageal cancer targeting nano-tablet based on a traditional Chinese medicine composition and a preparation method of the esophageal cancer targeting nano-tablet. The targeted nano tablet is prepared from the following refined extracts: a rhizoma paridis total saponin extract, a pseudobulbus cremastrae seu pleiones colchicine derivative extract, a panax notoginseng total saponin extract, a carthamin yellow extract, a thunberg fritillary bulb total alkaloid extract, frankincense-myrrh compound volatile oil, a resin extract and an astragaloside IV extract. The nano-carrier comprises a nano-carrier, a seaweed-laminarin composite extract, a processed rhizoma pinelliae total alkaloid extract and costus root volatile oil, and the costus root volatile oil serves as one of targeting ligands to be used for modifying the nano-carrier. The targeting property is strong: the dual-targeting ligand (costunolide-TOPK affinity peptide) on the surface of the nano-carrier can actively recognize and combine with a specific receptor of esophageal cancer cells, and meanwhile, due to the size (about 100-200 nanometers) of the carrier, the carrier is easy to enrich in tumor tissues through enhanced permeation and retention (EPR) effect, so that the modernization upgrading of the idea of'channel guiding medicine 'is realized.

The present disclosure provides compositions and methods related to tobacco plants. In particular, the present disclosure provides novel methods for producing tobacco plants and any related tobacco product having low nicotine alkaloid content. The tobacco plants produced according to the methods of the present disclosure exhibit reduced levels of nicotine alkaloids (e.g., nicotine) as compared to naturally occurring and transgenic tobacco plants, and thus represent commercially valuable substitutes for currently available tobacco varieties.

The present invention relates to sulforaphane glycoconjugate compounds, as well as to the synthesis and therapeutic uses thereof for treating inflammatory diseases.

The invention discloses an application of a marine fungus source compound in preparation of a skin anti-aging product, the marine fungus source compound is tripeptide alkaloid, and the structural formula is shown in the specification. The preparation method has the advantage of being capable of being applied to the preparation process of skin anti-aging products.

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention belongs to the technical field of facility horticulture postharvest treatment, and particularly relates to a tomato postharvest quality regulation and control light supplementing system based on white light-metabolism key enzyme coupling activation and an application method thereof, and the tomato postharvest quality regulation and control light supplementing system comprises an enzyme activity targeting white light module, an enzyme activity feedback module and a fruit bearing regulation and control module. According to the postharvest tomato quality regulation and control light supplementing system based on white light-metabolic key enzyme coupling activation, a white light spectrum segmentation-key enzyme activity dynamic matching coupling mechanism is innovatively designed by combining the regulation and control rule of white light on the activity of key enzymes such as carotenoid synthetase and phenylpropane metabolic enzyme of tomato fruits; through an adjustable broad-spectrum white light source and an enzyme activity feedback module, directional accumulation of beneficial metabolites such as carotenoids and flavonoid compounds and efficient degradation of alkaloids are realized.

The invention relates to a method for detecting alkaloid compounds based on UPLC-MSMS (ultra performance liquid chromatography-mass spectrometry) and application. The method comprises the following steps: after a sample is extracted, detecting a sample extracting solution and a standard solution by adopting an ultra performance liquid chromatography-mass spectrometry combined method; a mobile phase adopted by the ultra-high performance liquid chromatography is as follows: a mobile phase A is an aqueous solution containing 0.1% of formic acid; the mobile phase B is an acetonitrile solution containing 0.1% of formic acid. According to the method disclosed by the invention, after the extracted tissue sample is subjected to liquid chromatography separation, the alkaloid metabolites are qualitatively and quantitatively detected in a targeted manner, and due to the high selectivity of the high-resolution mass spectrum, parameter optimization does not need to be performed on each metabolite.

The invention relates to the technical field of scalp care, and discloses a scalp itching relieving composition and application thereof.The scalp itching relieving composition is mainly composed of forsythin, isatis tinctoria extract, cinchona alkaloid, a pH regulator and a solvent and can be used for shampoo, hair conditioners, scalp essence, scalp spray and hair masks. The fructus forsythiae phenol, the isatis tinctoria extract and the cinchona alkaloid in the scalp itching-relieving composition have a remarkable synergistic effect, and the scalp itching-relieving composition is efficient in itching-relieving effect, long-acting, mild, safe, low in irritation and suitable for various scalp types.

The invention discloses a short-contact washing agent for treating scalp seborrheic dermatitis and bacterial folliculitis, and relates to the technical field of external preparations and scalp care, the short-contact washing agent comprises the following components by weight: an active component combination: 0.01-0.05 wt% of halcinonide; 0.5 to 1.0 wt% of piroctone ethanolamine; 0.1 to 0.3 wt% of total alkaloids of sophora flavescens; 0.05 to 0.1 wt% of berberine; standardized traditional Chinese medicine effective part extracts are compounded with modern active ingredients, and the problem that the quality difference between batches of traditional Chinese medicine lotion is large is solved. The combination of a plurality of active components aims to synergistically act on a plurality of targets such as anti-inflammation, antibiosis and oil control, so as to comprehensively improve the scalp condition. In addition, the specific semi-solid colloid dosage form improves the staying performance of the product on the scalp, and aims to improve the refreshing feeling after use while ensuring the cleaning effect. The lotion provides a hormone-containing and hormone-free double-version design, and provides choices for requirements of different severity degrees and different use stages.

The application relates to the medical technical field, in particular to application of neoline in treatment of Parkinson's disease. 19 The C 19 The type-II diterpene alkaloid neoline can significantly improve movement disorder in a MPTP-induced Parkinson's disease model mouse, the drug efficacy is equivalent to that of the positive drug madopar, the effective dose is much lower than that of the positive drug, the administration mode is oral administration, drug compliance and use convenience are good, and the neoline can be used for development of anti-Parkinson's disease drugs.

The invention relates to an uncaria alkaloid extract as well as a preparation method and application thereof. The method comprises the following steps: heating and leaching uncaria stem and branch powder with acidic ethanol to obtain an acid alcohol leaching solution; carrying out reduced pressure distillation and concentration on the leach liquor to obtain an uncaria total alkaloid extract; removing acid-insoluble substances from the total alkaloid extract, degreasing, adjusting alkali, extracting with chloroform, and recovering a chloroform layer to obtain uncaria crude alkaloid; and dissolving the uncaria crude alkaloid in distilled water, adding into a macroporous resin chromatographic column, adsorbing, flushing, eluting by using a 20% ethanol solution with five column volumes, and collecting the eluent to obtain the uncaria alkaloid extract. The ramulus uncariae cum uncis alkaloid extract PURA1 has the effects of improving the pathological phenotype of the Alzheimer's disease model caenorhabditis elegans and improving the oxidative stress resistance of the caenorhabditis elegans, plays a role in prolonging the service life of the caenorhabditis elegans by regulating and controlling the p38MAPK signal channel of the caenorhabditis elegans, and can be used for preparing the medicine for relieving the Alzheimer's disease and resisting aging.

This invention discloses a dextrorotatory tetracyclic fused forsythoside alkaloid, its preparation method, and its anti-inflammatory and antioxidant applications, belonging to the field of medicinal chemistry. This dextrorotatory tetracyclic fused forsythoside alkaloid is a quinoline alkaloid with a structural skeleton consisting of two tetrahydrofuran rings fused together (a 6 / 6 / 5 / 5 structural skeleton) and an absolute configuration of 1'. S ,2' R ,3' S The molecular formula is C 17 H 20 NO. 6. The advantages of this invention are: An innovative extraction and separation process was used to efficiently isolate a dextrorotatory tetracyclic fused forsythoside alkaloid from the fruit of Forsythia suspensa. Alkaloids with this structural framework are extremely rare, and this is the first time it has been discovered in the Forsythia suspensa plant. Studies have found that this dextrorotatory tetracyclic fused forsythoside alkaloid possesses good anti-inflammatory and antioxidant activities, providing a new material basis for research on the anti-inflammatory and antioxidant activities of Forsythia suspensa.

The invention relates to the technical field of agricultural product processing, in particular to a preparation method and application of high-activity medicinal and edible lily tea drink dried flowers. The method solves the problem that active ingredients such as flavonoids, aromatics, steroid saponins and alkaloids are easy to lose in the existing dried lily preparation. In the prepared dried medical lily flowers, the relative concentration of honeysuckle glycoside is larger than or equal to 1460 micrograms / g, the relative concentration of benzene and substituted derivatives thereof is larger than or equal to 960 micrograms / g, the relative concentration of steroid sapogenin-O-glucose is larger than or equal to 2120 micrograms / g, the relative concentration of choline is larger than or equal to 699 micrograms / g, the medical value and edible quality of the dried medical lily flowers are remarkably improved, and the method is suitable for large-scale production.

The application discloses a supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion as well as a preparation method and application thereof. The water phase of the supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion is a purified lotus leaf peptide aqueous solution, and the oil phase is camellia seed oil loaded with narcotic alkaloids. The camellia seed oil not only serves as a fat-soluble carrier to improve the solubility of the narcotic alkaloids, but also has nursing effects due to the unsaturated fatty acids, vitamin E and phytosterols contained therein. The supramolecular assembly technology is used to mediate the lotus leaf peptide and the narcotic alkaloids to form high-performance emulsified particles, to replace synthetic surfactants to stabilize the Pickering emulsion, and to construct a natural emulsion system with high emulsification efficiency, high stability and high performance, so that the performance and the nursing effect of the emulsion are optimized.

The invention relates to the technical field of nitrogen heterocyclic compounds, in particular to nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the specification, wherein X is selected from any one of-OCH3,-OCH2CH3 and-O (CH2) 2CH3. An antiviral activity test result shows that the azacyclo-alkaloid series compounds provided by the invention have antiviral activity, and the antiviral activity level of part of azacyclo-alkaloid reaches or even exceeds that of a positive control drug. Therefore, the azacyclo-alkaloid is expected to be applied to prevention and treatment of plant viruses.

The invention belongs to the technical field of pharmacy, and particularly relates to application of polycyclic alkaloid in preparation of an anti-breast cancer drug, and the polycyclic alkaloid is 1, 2a, 7-trimethyl-5-tosyl-1, 2, 2a, 3, 4, 5-hexahydropyrrolo [4, 3, 2-de] quinoline. The polycyclic alkaloid compound is applied to preparation of anti-breast cancer drugs, the effect of inhibiting growth of breast cancer cells is remarkable, and the efficacy of the polycyclic alkaloid compound is far better than that of a positive drug ML162 aiming at EC50 (mu M) + / -S.D = 9.04 + / -0.37 of HCC1937 human breast cancer cells.

The application discloses a nitrogen acetylated carbazole alkaloid, and the structure is selected from one of the following structures: The application discloses a nitrogen acetylated carbazole alkaloid produced by fermentation of streptomyces J1074, and provides a biosynthetic preparation method, which is more green and environmental protection, and cost reduction compared with a chemical synthesis method; the compound ATC-A provided by the application shows significant antithrombotic activity in a thrombotic zebrafish model, which may be related to down-regulation of gene expression related to platelet activation and blood coagulation cascade, and has important development prospect and clinical application value.

The invention discloses a method for synthesizing novel alkaloid by enzyme catalysis of tryptamine and alpha-ketoamide substances, and the method comprises the following steps: reacting a compound shown as a formula (A) with a compound shown as a formula (B) in the presence of isoviscin synthetase to obtain alkaloid shown as a formula (C). The method is mild in condition, free of pollution and simple in process route.

The invention provides a broad-spectrum combined medicine for treating colorectal cancer. The broad-spectrum combined medicine is prepared from the following components in parts by weight: 100 parts of purified water, 38 to 90 parts of tripterygium wilfordii alkaloid, 38 to 90 parts of tripterygium hypoglaucum alkaloid, 28 to 85 parts of chamaejasmine, 28 to 85 parts of gelsmium elegans alkaloid and 28 to 85 parts of xanthoxylin. The medicine is prepared from the following raw materials in parts by weight: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata, 16 to 80 parts of astragaloside, 16 to 80 parts of oldenlandia diffusa alkaloid, 16 to 80 parts of sculellaria barbata alkaloid, 16 to 80 parts of tanshinone, 16 to 80 parts of carthamin, 16 to 80 parts of honeysuckle chlorogenic acid and 16 to 80 parts of pericarpium citri reticulatae flavone. By integrating anti-tumor components with multiple action mechanisms, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid and the like, tumor cell proliferation can be directly inhibited, and apoptosis can be induced; tanshinone, emodin and the like can inhibit tumor angiogenesis; ganoderma lucidum polysaccharides, astragaloside and the like can activate the immune system of the body, enhance the activity of T cells and NK cells, and form double strikes of direct killing and immune surveillance.

The invention discloses a high-nutrition chicken fermented feed formula containing dendrobium officinale tender leaves parasitized on taxus chinensis and taxus chinensis tender leaves, and belongs to the technical field of livestock and poultry feed. According to the formula, physiological requirements of chickens in different growth stages are taken as a core, dendrobium officinale tender leaves and taxus chinensis tender leaves parasitizing on taxus chinensis are taken as key functional auxiliary materials, basal feed, other functional auxiliary materials and fermentation strengthening elements are matched, and the feed is prepared through a specific fermentation process. Through fermentation, the absorption rate of nutritional ingredients in the auxiliary materials is increased, effective accumulation of natural flavor substances is achieved, meanwhile, the alkaloid content of taxus chinensis is strictly controlled to be smaller than or equal to 0.01%, and use safety is guaranteed. The feed meets the nutritional requirements of chickens and the taste and quality of chicken and egg products, and is suitable for the breeding requirements of different growth stages of broilers and different egg laying stages of laying hens.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.