1047 results about "Alkaloid" patented technology

The invention discloses a preparation method of four new skeleton alkaloid compounds with anti-inflammatory effect in the root of Anachymin and the use of the anti-inflammatory activity thereof. The four new skeleton alkaloid compounds are extracted from the root (root of Anachymin) of Roman pyrethrum (Anacyclus pyrethrum (L.) DC.) by using an organic solvent, and then separated by two or three methods of normal phase silica gel column chromatography, reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain eight new skeleton alkaloid monomer compounds, and the structures thereof are identified by high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy and X-ray single crystal diffraction. The invention also provides the NO inhibition effect of these compounds.

The present disclosure relates to tobacco plants, plant parts, seeds, compositions and methods related to modulating expression of novel genes in tobacco to modulate alkaloid levels.

The invention relates to the technical field of ferula asafoetida endophytic fungi separation and purification, in particular to alkaloid compounds in ferula asafoetida endophytic fungi as well as a preparation method and application of the alkaloid compounds in the ferula asafoetida endophytic fungi. And extracting with dichloromethane to obtain dichloromethane part extract, and purifying and separating the dichloromethane part extract by adopting silica gel column chromatography and high performance liquid chromatography to obtain the alkaloid compounds in the endophytic fungi of ferula sinkiangensis. The alkaloid compound in the ferula sinkiangensis endophytic fungi is disclosed for the first time, the compound is subjected to an in-vitro anti-tumor pharmacodynamic experiment, and according to the experiment result, the alkaloid compound has a certain inhibition effect on MCF-7 cells; therefore, the alkaloid compound can be used for preparing the medicine for preventing and treating the breast cancer, and a new medicine is provided for preventing and treating the breast cancer.

The invention discloses a temperature-sensitive hydrogel based on Chinese prickly ash alkaloid. The temperature-sensitive hydrogel is prepared from poloxamer 407, poloxamer 188 and Chinese prickly ash alkaloid powder. The temperature-sensitive hydrogel based on the pepper alkaloid prepared by the invention has three characteristics of biological adhesion, self-healing property and slow release property, and solves the technical problems that the pepper alkaloid is poor in stability and uncontrollable in drug release.

The invention relates to the technical field of ferula asafetida endophytic fungus strain separation and purification, in particular to an alkaloid component in pleurotus nebrodensis as well as a preparation method and application thereof, and the alkaloid component in pleurotus nebrodensis, namely an alkaloid compound in pleurotus nebrodensis, is prepared by the following steps: crushing pleurotus nebrodensis sporocarp, adding methanol, heating, refluxing and extracting; dispersing the pleurotus nebrodensis total extract into a suspension with water, and extracting to obtain a petroleum ether part and ethyl acetate part extract; and carrying out gradient elution on the ethyl acetate part extract by silica gel column chromatography, and separating and purifying the third fraction by high performance liquid chromatography to obtain the alkaloid compounds in pleurotus nebrodensis. The invention discloses the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C for the first time, and the pleurotus nebrodensis alkali C has a certain inhibition effect on HGC-27 cells, so that the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C, can be applied to the preparation of tumor prevention drugs and antitumor drugs.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention provides a marine biological alkali compound. The chemical formula of the marine biological alkali compound is as follows: # imgabs0. The marine biological alkali compound or pharmaceutically acceptable salt thereof can be used for preparing antibacterial drugs or anti-infective drugs. The invention also provides a separation and extraction preparation method or a chemical synthesis preparation method of the marine alkaloid compound or the pharmaceutically acceptable salt thereof, and the preparation method is simple and easy to industrialize. The invention also provides application of the marine biological alkali compound or the pharmaceutically acceptable salt thereof in preparation of antibacterial drugs or anti-infection drugs for human or fish.

The invention provides an alkaloid compound as well as a preparation method and an application thereof in tumor resistance. The alkaloid compound provided by the invention shows a remarkable cytotoxic effect on human non-small cell lung cancer cells A549, mouse lung adenocarcinoma cells Lewis, human triple negative breast cancer cells MDA-MB-231 and mouse breast cancer cells 4T1, and can be used for preparing medicines for treating breast cancer, lung cancer and the like.

The invention relates to the technical field of microbiology and food processing, in particular to pediococcus acidilactici and application of the pediococcus acidilactici in preparation of a brain-intestinal axis regulating efficacy metagen product.The high-activity pediococcus acidilactici DY-1 is separated out from natural fermentation pickles of super-polyselenium plant cardamine violifolia for the first time; the strain can be widely applied to fermentation of high-selenium plant-based food, can significantly promote glycolysis release of selenium-containing active substances, has good acid resistance, high protease activity and high lipase activity, can fully ferment plant-based fermentation raw materials, and has high yield. Glycolysis of macromolecular substances such as protein, saccharides and fat is facilitated, accumulation of active ingredients such as essential amino acid, unsaturated fatty acid, alkaloid and phenolic substances is promoted, and the edibility and functionality of the product are improved; and the organic selenium-rich metagen product prepared by sterilizing the fermentation mixture can synergistically improve human body cognition and emotion regulation regulated by microorganism-intestinal-brain axis.

The invention discloses a Lactobacillus buchneri strain with high yield of gamma-aminobutyric acid and application of the Lactobacillus buchneri strain in preparation of nutritional and healthy fruit and vegetable juice. The strain is Lactobacillus buchneri GM3, and is preserved in the Guangdong Microbial Culture Collection Center on January 9, 2025, and the preservation number of the strain is GDMCC No: 65737. The invention further discloses a preparation method of the Lactobacillus buchneri strain. The strain not only has the capacity of producing gamma-aminobutyric acid at high yield, but also has good acid resistance, cholate resistance and gastrointestinal fluid resistance, and is very suitable for fermentation production of fermented fruit and vegetable juice. The contents of gamma-aminobutyric acid and flavonoid substances in fruit and vegetable juice fermented by the strain are greatly increased, and the nutritional value of the fruit juice is remarkably increased; moreover, the contents of alkaloid substances and total sugar are remarkably reduced, so that the tea is healthier, and the flavor is better and more unique. The Lactobacillus buchneri GM3 is a safe lactic acid bacterium, and the Lactobacillus buchneri GM3 and the technology for producing fruit and vegetable juice through fermentation of the Lactobacillus buchneri GM3 have very good application value and market prospect.

The invention discloses medicinal and edible mulberry leaf drink substitute tea and a processing method thereof, and belongs to the technical field of mulberry leaf tea drink production. The processing method comprises the following steps: picking mulberry leaves, rinsing with ice water, carrying out synergistic fixation with ginger juice and steam, rolling and forming, dipping with a mulberry polysaccharide-iron compound solution in combination with a low-frequency electromagnetic field, and then treating with a staged oxygen-controlled fermentation process. Wherein the ginger juice steam enzyme deactivation system accelerates enzyme deactivation through the synergistic effect of ginger volatile oil and high-temperature steam under the conditions that the temperature is 105-110 DEG C and the pressure is 0.10-0.25 MPa; the mulberry polysaccharide-iron compound permeates into mulberry leaf cells under the assistance of a low-frequency electromagnetic field, and synthesis of gamma-aminobutyric acid is promoted; in the primary fermentation stage, eurotium cristatum is utilized to carry out anaerobic metabolism under mediation of Fe < 3 + >, and in the secondary fermentation, a metabolic pathway is optimized through combination of lactobacillus plantarum and bacillus subtilis. The prepared tea substitute is rich in GABA (gamma-aminobutyric acid), flavonoid substances and alkaloid substance DNJ, has the physiological effects of reducing blood sugar and blood fat, and can relieve pancreas burden.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an inhalation type pharmaceutical composition for treating inflammatory respiratory diseases and a preparation method thereof. The inhalation type pharmaceutical composition comprises an active component and a nano-liposome, and mannose is modified on the surface of the nano-liposome; the active ingredients comprise bulbus fritillariae cirrhosae total alkaloids and platycodin D; the nano-liposome is prepared from the following raw materials: phospholipid, cholesterol and cholesterol-polyethylene glycol 1000-mannose ester. The liposome can be specifically recognized by a mannose receptor highly expressed on the surface of alveolar macrophage through a surface-modified mannose ligand, so that the receptor-mediated endocytosis is started, and the wrapped bulbus fritillariae cirrhosae total alkaloids and platycodin D are efficiently introduced into cells. The inhaled pharmaceutical composition of the present invention collectively pushes macrophages from a pro-inflammatory M1 phenotype to a repairable M2 phenotype. Therefore, the overall curative effect of the combination of the two components is far better than that of the independent use of any component.

The invention discloses a preparation method and application of an areca total alkali extract. The areca total alkali is calculated by arecoline, arecoline, demethylated arecoline and demethylated arecoline. The preparation method comprises the following steps: carrying out water extraction on areca catechu, purifying an extracting solution through cationic resin, concentrating an eluent, regulating acid, carrying out suction filtration, and drying to obtain the areca catechu total alkali extract. According to the preparation method, high-purity alkaloid can be obtained from areca nuts, the process is simple, convenient and stable, the cost is low, the preparation method can be widely applied to industrial production, and the preparation method has the effects of improving rumen fermentation characteristics, protecting rumen health, improving nutrient digestibility, promoting animal growth and improving animal production performance when applied to economic animals.

Methods for treating a neuropsychiatric disorder by administering an iboga alkaloid and a cardioprotective agent in conjunction with analysis of brain image data is described. Also described are methods to improve brain health and to slow or reverse brain aging by disorder by administering an iboga alkaloid and a cardioprotective agent, where analysis of brain image data is used to monitor and / or evaluate treatment effectiveness.

The invention discloses mulberry leaf flavone as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction and preparation. Flavone is released through a microwave-assisted enzyme method, flavone is dissolved through a deep eutectic solvent, the reaction rate is increased through microwave treatment, meanwhile, the catalytic activity of enzyme can be enhanced, and the enzymatic reaction speed and yield are effectively increased; the deep-eutectic solvent contains a large amount of hydroxyl groups and amino groups, the hydroxyl groups and the amino groups can easily form hydrogen bonds with flavone, the solubility of the flavone in the deep-eutectic solvent can be increased, and meanwhile the total extraction amount of the flavone is increased under the dual auxiliary effects of microwaves and enzyme; by adopting a resin separation method, high-purity mulberry leaf flavone, mulberry leaf alkaloid and mulberry leaf polysaccharide can be obtained. The mulberry leaf flavone and the mulberry leaf alkaloid which are prepared and separated by the method can be conveniently and rapidly proportioned according to the requirements, and the mulberry leaf flavone and the mulberry leaf alkaloid can be widely applied to the aspect of hypoglycemic food.

The present invention belongs to the technical field of drug or functional food application. The present invention discloses a compound of the following structural formula and the use of the compound in preparing a drug for treating fatty liver disease or a functional food for alleviating fatty liver disease. Also disclosed is Eurotium cristatum ( Eurotium cristatum )SXHBTBU1934 and a method for preparing the compound.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

Methods for making wettable, dispersible potato protein powders are provided. The wettable dispersible potato protein powders comprise potato protein, free amino acids, non-protein nitrogen compounds, and minerals from potatoes; and are low in total α-glycoalkaloids. The inventive potato protein powders are suitable for use in formulating foods, feeds and beverages.

The invention discloses a method for simultaneously extracting and separating flavonoid and alkaloid components in mulberry leaves, which comprises the following steps: firstly, adjusting the pH value of an aqueous solution of a deep eutectic solvent to form a uniform single-phase solution, adding mulberry leaf powder into the single-phase solution, carrying out ultrasonic extraction, centrifuging after the extraction is finished, and taking a supernatant; adjusting the pH value of the extracting solution again to form a two-phase system, and respectively obtaining an upper-layer eutectic solvent phase and a lower-layer water phase. The conditions of the extraction step are as follows: the concentration of the eutectic solvent is 30%-70%, the solid-to-liquid ratio is 1: 5 g / mL-1: 40 g / mL, the extraction time is 20 min-100 min, and the extraction pH is 5.5-9.5; in the separation step, the conditions of the separation step are as follows: the separation pH is 1-5. Through HPLC (High Performance Liquid Chromatography) analysis, a flavonoid component (isoquercitrin) is mainly distributed in an upper-layer deep-eutectic solvent phase, an alkaloid component (deoxynojirimycin) is mainly distributed in a lower-layer water phase, and the reusability is good.

Compositions and methods for genetically modifying the production levels of nicotine and other alkaloids in plants are provided.

The invention belongs to the technical field of natural medicines, and particularly relates to application of spermidine alkaloid in preparation of anti-tumor medicines and / or reversion of drug resistance. The invention provides an application of macrocyclic spermidine alkaloid in preparation of anti-tumor drugs and / or reverse drug resistance. The macrocyclic spermidine alkaloid comprises one or more than two of 13-oxo-furan celastrus orbiculatus, furan celastrus orbiculatus, benzene-substituted celastrus orbiculatus and celastrus orbiculatus cinnamamide alkali. The macrocyclic spermidine alkaloid disclosed by the invention has the characteristics of broad-spectrum anti-tumor effect, good anti-tumor effect, relatively low toxic and side effects, capability of reversing 5-fluorouracil drug resistance, relatively high water solubility, relatively easy source, simple structure, only one chiral carbon in the structure, relatively easy artificial synthesis, intervention in cell autophagy mechanism and the like, so that the overall treatment cost is relatively low; the method has a potential important clinical application prospect.

The invention discloses application of a cytisine type alkaloid and a derivative thereof in preparation of a medicine for preventing or treating respiratory syncytial virus infection, and relates to the technical field of respiratory syncytial virus prevention and treatment. The research finds that the natural product cytisine shows good anti-respiratory syncytial virus infection activity in vivo and in vitro, and finds that the cytisine can obviously relieve lung injury caused by virus infection and reduce lung inflammation; the invention provides an experimental basis for developing an efficient and safe drug for resisting respiratory syncytial virus (RSV) infection. As a natural product, the cytisine reduces the social burden caused by treatment of respiratory syncytial virus infection and reduces the treatment cost.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a coptis chinensis extraction process which comprises the following specific steps: coptis chinensis pretreatment: selecting dried coptis chinensis rhizomes, removing impurities and mildewed parts, washing the treated coptis chinensis twice with deionized water, removing surface dust, drying in a drying oven at 50-60 DEG C until the water content is less than 5%, and taking out the coptis chinensis; the dried coptis chinensis is smashed through a smashing device, the smashed coptis chinensis is sieved through a 80-100-mesh sieve, and uniform fine powder is obtained.In the coptis chinensis extraction technology, interference of fat-soluble impurities is reduced through degreasing pretreatment, the subsequent resin adsorption efficiency is improved, pigment and polar impurities can be selectively adsorbed through decoloration of resin, effective ingredients such as alkaloid can be reserved, and the extraction efficiency of coptis chinensis is improved; small molecular impurities can be accurately removed through a membrane separation technology, traditional activated carbon adsorption is replaced, loss of effective components is reduced, finally, efficient separation and purification are conducted through an acid-base precipitation method, and water-soluble impurities are synchronously removed.

The invention relates to the technical field of dendrobium officinale processing, and particularly discloses a method for realizing high value-added conversion of dendrobium officinale alkaloid based on efficient extraction. The method for realizing high value-added conversion of dendrobium officinale alkaloid based on efficient extraction comprises the following steps: S1, pretreatment and activation of raw materials: crushing dried dendrobium officinale stalks to 60-100 meshes to obtain dendrobium powder; mixing the dendrobium nobile powder with an activating agent aqueous solution to obtain activated wet dendrobium nobile powder; s2, deep eutectic solvent synergistic microwave extraction; s3, precipitation and enrichment of an anti-solvent; s4, cleaning and drying the precipitate; s5, preparing alkaloid nanoparticles; and S6, preparing the functional health-care product preparation. As a functional dietary supplement, the dendrobium officinale alkaloid solid beverage tablet or capsule provided by the invention can be used for daily health care, and has the advantages of high bioavailability, strong intestinal synergistic regulation function and good physical stability.

The invention discloses a broad-spectrum combined medicine for treating liver cancer, which comprises purified water, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid, chamaejasmine, gelsmium elegans alkaloid, xanthoxylin and various B medicines, and further comprises folic acid, vitamins, copper sulfate, zinc sulfate, manganese sulfate, yeast and penicillin sodium. Through the multi-component, multi-target and multi-path design, the medicine disclosed by the invention shows a potential effect of inhibiting the progress of liver cancer and good safety in cell experiments, animal models and human individual case observation. Cellular immunity and humoral immunity can be enhanced, the treatment cost is lower than that of a traditional treatment scheme, and no pain is caused during treatment.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention provides lotus seed wine and a preparation method thereof.The lotus seed wine is prepared through the steps of fermentation main material pretreatment, fermentation auxiliary material pretreatment, vertical imgabs0 # fermentation wine production, continuous imgabs1 # fermentation wine production, storage and blending. The lotus seed wine is high in wine yield and has high-content bioactive substances and flavor substances, particularly, the content of alkaloid in the lotus seed wine is obviously increased, the wine body is mellow, soft, sweet, full, fine, harmonious and refreshing, and the aftertaste of the lotus seed is long-lasting.

The application relates to a preparation method of a fritillaria cirrhosa bulbus extract. Step one: drying and crushing fritillaria cirrhosa bulbus to obtain fritillaria cirrhosa bulbus powder, taking the fritillaria cirrhosa bulbus powder and a cyclodextrin powder to stir-fry a mixture one; step two: adopting ethanol normal-temperature reflux extraction on the mixture one to separate supernatant one and filter residue; step three: recovering and drying the filter residue in step two, taking the dried filter residue and the cyclodextrin powder to stir-fry a mixture two; step four: adopting ethanol and organic acid normal-temperature reflux extraction on the mixture two; step five: combining the supernatant one and supernatant two, adding into a macroporous adsorption resin impurity removal device, then adding ethanol into the macroporous adsorption resin impurity removal device until the eluent is colorless; concentrating and drying to obtain the fritillaria cirrhosa bulbus extract. The application can obviously improve the extraction efficiency of total alkaloids of fritillaria cirrhosa bulbus and has a good application prospect.

The invention discloses a cephalotaxus hainanensis Ch2OGD1 gene and application thereof, and belongs to the technical field of gene engineering. The Ch2OGD1 gene is used for coding a 2-oxoglutaric acid dependent dioxygenase (2OGD) type Pictet-Spengler enzyme, and the Pictet-Spengler enzyme is used for coding a Pictet-Spengler enzyme. Through a tobacco transient expression system and in-vitro enzyme activity determination, the Ch2OGD1 is proved to be capable of specifically catalyzing the condensation of dopamine and 4-hydroxyphenylpropanal to generate 1-phenethyl isoquinoline. The invention provides a directional preparation method of 1-phenethyl isoquinoline based on the Ch2OGD1 gene, provides a key enzyme element for biosynthesis of a cephalotaxine alkaloid precursor compound, and provides a technical basis for expanding the application of the gene in plant genetic engineering. And a potential technical path is provided for relieving the problem of resource shortage of cephalotaxine alkaloids.

The invention relates to the field of natural medicines, and discloses an alkaloid and a preparation method and application thereof, and the alkaloid has a structure as shown in a formula I and a pharmaceutically acceptable salt thereof. The alkaloid is separated from an n-butyl alcohol extraction part of a dendrobium huoshanense stem ethanol extract through silica gel column chromatography and a preparative thin-layer separation technology, and the structure of the alkaloid is identified through nuclear magnetic resonance and high-resolution mass spectrometry. Cell experiments prove that the alkaloid prepared by the invention can remarkably relieve inflammation induced by LPS, inhibit proliferation of human gastric cancer cells (AGS) and promote apoptosis of the AGS; molecular docking analysis shows that the alkaloid prepared by the invention can be remarkably docked with active sites of inducible iNOS, IL-6, TNF-alpha and MMP9, and the binding energy is 9.5-8.6 kcal / mol.