The invention belongs to the field of bioengineering, and relates to a main stream-type carbon dioxide concentration measurement device, which comprises an infrared light source, a gas detection module, a filter circuit, an amplifying circuit, a calculation unit, a main gas channel and a measurement channel, wherein the gas detection module comprises a main measurement end, a reference end, and a temperature sensor arranged between the main measurement end and the reference end; the infrared light source is positioned on one side of the main gas channel; the main measurement end and the reference end are close to each other and are positioned on the other side of the main gas channel; and the measurement channel is vertical to the main gas channel, and is provided with a light source filter, a receiving end filter, a main measurement end filter and a reference end filter. The main stream-type carbon dioxide concentration measurement device has the characteristics of rational and clear light path design, simple operation, quick reaction response, convenient and fast cleaning and replacement, and higher timeliness and accuracy.