37 results about "Arecoline" patented technology

The invention relates to a buccal betel nut bag and a preparation method thereof. The betel nut bag comprises betel nut composition particles and bags, the betel nut composition comprises, by weight, 60-75% of betel nut-beta-cyclodextrin inclusion compounds, 5-10% of betel leaves, 0-10% of adhesives, 8-15% of betel nut essence, 2-8% of pH regulators and 1-8% of wetting agents, the tannin content in the betel nut extracts is 0-5% of tannin in betel nut raw materials, and the tannin content in the betel nut raw materials is 0-5% of tannin in the betel nut raw materials. The content of arecoline is 1-20% of that of arecoline in the betel nut raw material. The buccal betel nut bag disclosed by the invention has the effects of reducing the addiction of betel nuts, freshening breath, refreshing mind and restoring consciousness, maintaining the flavor of the betel nuts and reducing damage to the oral cavity and heart and brain.

The invention discloses a preparation method and application of an areca total alkali extract. The areca total alkali is calculated by arecoline, arecoline, demethylated arecoline and demethylated arecoline. The preparation method comprises the following steps: carrying out water extraction on areca catechu, purifying an extracting solution through cationic resin, concentrating an eluent, regulating acid, carrying out suction filtration, and drying to obtain the areca catechu total alkali extract. According to the preparation method, high-purity alkaloid can be obtained from areca nuts, the process is simple, convenient and stable, the cost is low, the preparation method can be widely applied to industrial production, and the preparation method has the effects of improving rumen fermentation characteristics, protecting rumen health, improving nutrient digestibility, promoting animal growth and improving animal production performance when applied to economic animals.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

The utility model relates to the technical field of betel nut Chinese patent medicine production, and discloses an extraction device for betel nut secondary alkali detection of betel nut-containing Chinese patent medicine, which comprises a bottom plate, the upper side of the bottom plate is fixedly connected with a support frame, the inside of the support frame is fixedly connected with a box body, and the lower side of the box body is fixedly connected with a purification box. The device comprises a box body, the upper side of the box body is fixedly connected with a conveying assembly, the conveying assembly is used for conveying an extracting solution, the front side of the box body is rotatably connected with a first box door, the two sides of the rear portion of the box body are fixedly connected with hydraulic rods, the output ends of the hydraulic rods are fixedly connected with a supporting plate, and the lower side of the supporting plate is fixedly connected with a brush. According to the device, impurities can be effectively removed, the filtering assembly can be conveniently cleaned, then the purity of an extract can be guaranteed, the device is provided with a refrigeration structure, and qualitative change of an extracting solution caused by the fact that the interior of the device is too hot is prevented.

The invention discloses arecoline tartrate solid salt as well as a preparation method and application thereof. The preparation method comprises the following preparation steps: S10, adding tartaric acid into a first solvent, heating and stirring to obtain a tartaric acid solution; s20, adding the arecoline solution into the tartaric acid solution, and uniformly stirring and mixing to obtain a salt solution; s30, adding a second solvent into the salt solution, uniformly stirring and mixing, and heating for reaction to obtain a solid-liquid mixture; s40, adding a third solvent into the solid-liquid mixture, and pulping to obtain slurry; and S50, washing and filtering the slurry with a fourth solvent to obtain a filter cake, and carrying out vacuum drying on the filter cake to obtain the arecoline tartrate solid salt. According to the technical scheme, the arecoline tartrate solid salt is prepared through a simple process, the stability of arecoline can be improved, so that in further application of buccal tobacco products, arecoline is uniformly, slowly and durably released in the oral cavity, long-acting and mild physiological satisfaction is provided for consumers, and meanwhile irritation to digestive tracts can be reduced.

The invention belongs to the technical field of disease prevention and control in aquaculture, and discloses application of arecoline derivatives in killing Bowden worms. A coarse screening experiment, a soaking toxicity test and a dose effect experiment prove that in coarse screening, 0.5 mg / L of the arecoline derivative is soaked for 90 min, so that the killing rate of the arecoline derivative on the bodworm reaches 75%, and the arecoline derivative is obviously superior to the other five kinds of arecoline and derivatives thereof such as triclabendazole and arecoline; a soaking toxicity test shows that after the arecoline derivative with the concentration of 0.7 mg / L is soaked for 90 min, the survival rate of the trachinotus ovatus is 100%, and no remarkable toxicity exists; a dose effect experiment further proves that the killing rate of 0.7 mg / L of the arecoline derivative on the boxworm is increased to 95.35%. The arecoline derivative has a remarkable effect of killing the boxworm, is high in safety to host fishes, has no obvious residual risk, can be further researched and developed to be special for preventing and controlling the boxworm in aquaculture, provides an effective scheme for solving culture diseases caused by the boxworm, and has a wide application prospect.

The present invention discloses a traditional Chinese medicine preparation for preventing and / or treating myocardial infarction, its preparation method and application, belonging to the technical field of pharmaceuticals. The traditional Chinese medicine is obtained by extracting Areca catechu with 20% - 95% ethanol. The present invention first reveals that the Areca catechu extract has an anti-myocardial infarction effect. The experimental results show that the crude extract of Areca catechu can significantly inhibit H2O2-induced cardiomyocyte death, improve the left ventricular ejection fraction of myocardial infarction mice, and reduce the myocardial infarction area, and the effect is equivalent to that of the positive drug Suxiao Jiuxin Pills, while arecoline has no cardiomyocyte protection effect. The present invention is of natural origin, simple to prepare, and low in cost, and can be used as a new supplement or alternative for clinical prevention and treatment of myocardial infarction, and has important clinical value and application prospects.

The invention relates to the field of oral disease prevention and treatment, in particular to oral ulcer gargle containing sodium surfactin, which comprises polyvinylpyrrolidone, carboxymethyl cellulose sodium, glycerol, N-acetamido glucose, sorbitol, saccharin sodium, lactic acid, sodium surfactin and purified water. The sodium bacillus subtilis lipopeptide is combined with pathogenic microorganisms and potential cancerous cells through electrostatic attraction, precise killing is achieved, PVP and CMC-Na form a physical protection layer on the ulcer surface to isolate irritants and relieve pain, sodium bacillus subtilis lipopeptide and lactic acid have a synergistic effect, cancerogenic substances such as arecoline in the oral cavity can be wrapped or degraded, the cancerous risk is reduced, and the cancerous effect is improved. And the components are physiologically inert, have good biocompatibility and do not irritate oral mucosa.

The invention discloses an arecoline complete antigen as well as a preparation method and application thereof. According to the arecoline complete antigen, the coupling position of the hapten is at the N site of an arecoline molecule main body ring. The arecoline complete antigen has the advantages that the connection point retains the spatial conformation of the arecoline antigen, and compared with a vaccine prepared from a complete antigen taking carboxyl of arecoline as a coupling site, an antibody initiated by the vaccine prepared from the arecoline complete antigen synthesized through the N connection point has higher affinity to arecoline; and the generated antibody can crossly recognize demethylated arecoline. The vaccine prepared from the arecoline complete antigen disclosed by the invention can be used for effectively relieving body temperature reduction caused after mice take in arecoline and has an analgesic effect. The arecoline complete antigen disclosed by the invention can be used for treating betel nut addiction patients and developing monoclonal antibodies, subsequent related kit products and the like.

The invention discloses arecoline type triazole glycoside compounds and application thereof in preparation of weight-reducing drugs, the structural formula of the compounds is as follows: in the formula, R represents glycosyl, arecoline is used as a starting raw material, methyl is removed from carboxylic acid methyl ester group or N-methyl group, then the carboxylic acid methyl ester group or N-methyl group is converted into propyne, and the propyne is converted into propargyl; and carrying out a click reaction with an azido sugar compound to introduce different sugar units. Pharmacological activity tests show that the compound has good inhibitory activity on lipase, which indicates that the arecoline type triazole glycoside compound can be used as a novel weight-reducing drug for research and utilization.

The invention discloses an aryl isoxazole (line) arecoline derivative and its application, and relates to the technical field of chemical pesticides. The aryl isoxazole (line) arecoline derivative has a general formula shown in formula (I): wherein the definitions of the groups in the formula are as shown in the specification. The present invention adopts the above-mentioned aryl isoxazole (line) arecoline derivative and its application, has a brand-new molecular structure, can significantly enhance the antibacterial activity of the arecoline parent, has a good inhibitory effect on plant fungal diseases such as pepper phytophthora, pitaya black spot fungus, mango stem rot fungus, banana anthracnose fungus, phomopsis, wheat fusarium, rice sheath blight fungus, rice blast fungus, rapeseed sclerotinia fungus, apple ring rot fungus, banana wilt fungus, etc., and can be used for the development and use of effective ingredients for preparing agricultural fungicides or adjuvants.

The utility model relates to the technical field of demethylated arecoline detection, and discloses a reagent titration device for demethylated arecoline detection containing betel nut Chinese patent medicine, which comprises a bearing frame, the left side of the bearing frame is fixedly connected with a bearing plate, the upper side of the bearing plate is fixedly connected with a liquid storage cylinder, the upper side of the liquid storage cylinder is fixedly connected with a connecting assembly, and the connecting assembly is fixedly connected with the liquid storage cylinder. The connecting assembly is used for conveniently adding raw materials, a supporting frame is fixedly connected to the lower side of the bearing plate, a motor is fixedly connected to the interior of the supporting frame, a rotating shaft is fixedly connected to the output end of the motor, and a fixing sleeve is fixedly connected to the exterior of the rotating shaft; and fixing blocks which are uniformly distributed are fixedly connected to the outer part of the fixing sleeve. According to the device disclosed by the utility model, raw materials in the liquid storage cylinder can be fully and uniformly stirred, an experimenter does not need to manually shake a reagent bottle, and the dropping speed of a stock solution can be conveniently and accurately controlled to drop a standard solution.

The invention relates to application of arecoline derivatives in prevention and treatment of plant viruses and pathogenic bacteria, and finds that the arecoline derivatives I-1-I-14 show good anti-plant virus and pathogenic bacteria activity for the first time, and can well inhibit tobacco mosaic virus (TMV), cucumber fusarium wilt, peanut brown spot, apple ring rot, wheat sheath blight, tomato early blight, rice blast, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici and phytophthora capsici, and the like. And sclerotium of rape has eight plant germs.

The application belongs to the technical field of heterocyclic compounds, and particularly relates to a preparation method of arecoline and a preparation method of a base material of a betel quid. 1,2,5,6-tetrahydropyridine and formic acid are dissolved in water, an aqueous formaldehyde solution is added after being heated, and then the mixture is kept warm, the pH is adjusted, and an organic layer is extracted. The organic layer is spin-dried to obtain N-methyl tetrahydropyridine. The N-methyl tetrahydropyridine is dissolved in N,N-dimethylacetamide, a palladium catalyst, a palladium catalyst ligand and an organic base catalyst are added, and then the mixture is heated after being replaced by nitrogen. Methyl chloroformate is added under the protection of nitrogen to react, and then the mixture is cooled and filtered to obtain a filtrate. Methyl sulfonic acid is added to the filtrate to react, and then the mixture is cooled and filtered to obtain arecoline methanesulfonate. The arecoline methanesulfonate is added to water, the pH is adjusted, and then the mixture is extracted. The obtained organic layer is concentrated to obtain arecoline crude product, and then the arecoline crude product is subjected to negative pressure rectification to obtain pure arecoline. The process flow is greatly simplified, the production conversion is facilitated, the yield of the prepared arecoline is high, and the purity of the prepared arecoline is high.

The invention provides a preparation method of an areca-nut core extract, which comprises the following steps: crushing areca-nut cores through a crusher, mixing the crushed areca-nut cores with water, heating and stirring in cooperation with a stirrer, and subsequently extracting to obtain an areca-nut extracting solution. Compared with the prior art, the method has the following beneficial effects that the water-phase material is subjected to refined filtration and macroporous resin adsorption so as to further remove arecoline dispute to body functions, condensed tannin with strong astringent taste and introduced sweetening agent components, and then the collected column passing liquid is subjected to refined filtration and ultrafiltration, so that the content of the astringent and the content of the sweetening agent components are further removed. The method can be used for removing possibly introduced resin scraps, macromolecular impurities polymerized in process treatment and the like, and controlling the pore size of a fine filtration and ultrafiltration membrane, so that macromolecular polysaccharide, protein, pectin and other components influencing the clarity of the extract can be more favorably removed.

The invention belongs to the technical field of medicines, and discloses a composition for improving bioavailability of arecoline. Specifically, the composition specifically comprises arecoline and magnolol, and the weight ratio of arecoline to magnolol in the pharmaceutical composition is (1: 0.1)-(1: 15). According to the composition, the in-vivo bioavailability of the arecoline can be remarkably improved, the half-life period and the average residence time of the arecoline can be prolonged, the application range of the arecoline is widened, and the treatment effect of the arecoline on diseases such as tapeworm infection, abdominal distension, diarrhea, edema, cancer, Alzheimer's disease, cerebral apoplexy, epilepsy and viral pneumonia can be better exerted.

The invention discloses a preparation method of betel nut powder. The preparation method comprises the following steps: adding water into fresh betel nut freeze-dried powder, and carrying out enzymolysis for 150-200 minutes by using a compound enzyme under the conditions that the pH value is 5.0-5.5 and the temperature is 40-45 DEG C; adding a carrier, sodium bicarbonate, lecithin and coconut oil into the obtained enzymatic hydrolysate, and homogenizing and emulsifying at the high pressure of 45-50MPa; and carrying out spray drying under the conditions that the air inlet temperature is 110-120 DEG C, the air outlet temperature is 60-65 DEG C and the rotating speed of an atomizer is 20000-25000rpm, and crushing to obtain powder with the particle size of less than 100nm. The invention further provides the prepared areca nut powder and candies containing the areca nut powder. According to the preparation method, loss of effective components such as arecoline in the preparation process is prevented. The prepared areca nut powder is high in content of active ingredients such as arecoline and the like, and is free of bitterness and astringent taste.

The invention relates to the technical field of arecoline concentration detection, in particular to an arecoline concentration detection method and an arecoline consumption evaluation method. The arecoline concentration detection method provided by the invention comprises the following steps: S1, adding an internal standard substance into a to-be-detected liquid, and acidifying to obtain the to-be-detected solution; s2, extracting the to-be-detected solution by adopting a solid-phase extraction method to obtain extract liquor; and S3, determining the concentration of arecoline in the extract liquor by adopting an ultra-high performance liquid chromatography-mass spectrometry method. According to the method disclosed by the invention, the to-be-detected solution is extracted and is matched with the ultra-high performance liquid chromatography-mass spectrometry, so that the arecoline concentration with an accurate result can be rapidly and efficiently detected, and the detection sensitivity on the arecoline concentration is high. Meanwhile, on the basis of a solid-phase extraction pretreatment method, the concentration of arecoline is detected in combination with an ultra-high performance liquid chromatography-mass spectrometer, the urban per capita consumption is calculated on the basis of sewage epidemiology (WBE), and the result is accurate.

The invention belongs to the technical field of heterocyclic compounds, and particularly relates to a preparation method of arecoline, a buccal bag base material and a preparation method of the buccal bag base material. The preparation method comprises the following steps: dissolving 1, 2, 5, 6-tetrahydropyridine and formic acid in water, heating, adding a formaldehyde aqueous solution, keeping the temperature, adjusting the pH value, extracting to obtain an organic layer, and spin-drying the organic layer to obtain N-methyltetrahydropyridine; the preparation method comprises the following steps: dissolving N-methyltetrahydropyridine in N, N-dimethylacetamide, adding a palladium catalyst, a palladium catalyst ligand and an organic base catalyst, heating after nitrogen replacement, adding methyl chloroformate under the protection of nitrogen for reaction, cooling and filtering to obtain filtrate; adding methanesulfonic acid into the filtrate for reaction, cooling and filtering to obtain arecoline mesylate; the method comprises the following steps: adding arecoline mesylate into water, adjusting the pH value, extracting, concentrating the obtained organic layer to obtain a crude arecoline product, and carrying out negative pressure rectification to obtain a pure arecoline product. According to the method, the technological process is greatly simplified, production conversion is facilitated, and the prepared arecoline is high in yield and purity.

The invention provides a betel nut flavored product. The betel nut flavored product comprises arecoline salt. According to the present invention, the arecoline salt is applied to the product, such that the user can ingest the arecoline during the smoking, the effect similar to the areca-nut chewing effect is produced, and the irritation is low.

This application provides a composition and a product thereof. The composition includes arecoline and an acid. The composition is convenient to use and widely used, with a low pH value and weak irritation.

The invention discloses a method for simultaneously detecting arecoline and arecoline in a biological sample and application of the method. According to the method, a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) is adopted for testing and analysis, an octadecyl bonded silica gel chromatographic column is used as a detection chromatographic column, 0.001% formic acid aqueous solution-acetonitrile is used as a mobile phase, a gradient elution procedure is adopted, and specific mass spectrometry conditions are matched, so that the arecoline and the arecoline in a biological sample can be quantitatively determined at the same time. The detection method is applied to pharmacokinetic research on arecoline and arecaine in a living body, and fills up the technical blank in the aspect of arecoline and arecaine detection at present.

The invention discloses a method for detecting arecoline residues in a biological sample and application of the method. According to the method, a high performance liquid chromatography-tandem mass spectrometry method is used for testing and analyzing, an octadecyl silane bonded silica gel chromatographic column is used as a detection chromatographic column, 0.1% formic acid water (A)-10mmol / L ammonium formate water (B) is used as a mobile phase, a gradient elution procedure is adopted, and specific mass spectrometry conditions are matched, so that the arecoline in a biological sample can be quantitatively determined. The detection method disclosed by the invention is applied to the detection research of the arecoline residues in the biological tissues, and fills the current technical blank in the aspect of detecting the arecoline residues in the biological samples, especially in the chicken edible tissues.

The invention provides arecoline hydrazide derivatives as well as preparation and application thereof in preparation of anti-inflammatory drugs, and relates to the technical field of drug compound synthesis. The arecoline hydrazide derivative has a structural formula as shown in the following formula (1), wherein R is phenyl or substituted phenyl. According to the invention, arecoline is used as a raw material, an ester group in arecoline is hydrolyzed to generate an arecoline intermediate, and arecoline carboxyl is further catalyzed to be subjected to a condensation reaction with various substituted hydrazine to obtain the arecoline hydrazide compound. The arecoline hydrazide derivatives of the series can remarkably inhibit release of an inflammatory medium NO in RAW264.7 cells induced by LPS and the expression level of inflammatory cytokines TNF-alpha and IL-6 protein; the compound has high in-vitro anti-inflammatory activity and low cytotoxicity, and can be used for preparing anti-inflammatory drugs.

The invention belongs to the technical field of medicines, and discloses an application of a natural product composition in medicines related to a COPD (chronic obstructive pulmonary disease) treatment effect. In particular, the disclosed composition is applied to preparation of drugs playing a protective role in preventing and / or treating pulmonary shortness of breath, lung injury, oxidative stress and inflammatory response caused by COPD. Pharmacological experiments prove that the four components of baicalein, arecoline, magnolol and isoquercetin in the composition can jointly exert anti-inflammatory and anti-oxidation effects in the treatment of COPD (chronic obstructive pulmonary disease), and meanwhile, the composition can improve the lung function and promote the repair of lesion lung tissues.

This invention utilizes *Allium macrostemon* to obtain its aqueous extract, petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract. The effects of these aqueous extracts and extracts of different polarities on the learning and memory functions of D-galactose-induced aging model mice were investigated using Y-maze, darkness avoidance, Morris water maze, and laser speckle cerebral blood flow assays. Reactive oxygen species (ROS), SOD, and MDA were also measured. Multiple experiments demonstrated that *Allium macrostemon* can improve memory function in mice. Furthermore, the effects of *Allium macrostemon* extract on arecoline- or oxidative tremor-induced tremor behavior in mice were studied. The results showed that *Allium macrostemon* extract can effectively shorten the duration of tremors, proposing a new application for *Allium macrostemon* extract in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases, providing broad application prospects for the treatment or adjuvant therapy of neurodegenerative diseases.

The invention relates to the technical field of arecoline application and particularly discloses application of arecoline in inhibition of African swine fever virus replication and proliferation and a method thereof, the arecoline is applied to an antiviral preparation, the concentration of the arecoline acting on porcine PK cells in the preparation is 400 micromol / L, the acting time is 24 h, the concentration of the arecoline acting on the porcine PK cells in the preparation is 400 micromol / L, and the concentration of the arecoline acting on the porcine PK cells in the preparation is The expression of the preparation for inhibiting the replication and proliferation of the African swine fever virus comprises reduction of transcription and expression levels of virus specific genes, and the virus specific genes comprise a P30 gene, a P54 gene and a P72 gene. The discovery fills the key blank of anti-ASFV research of natural compounds, provides brand-new natural candidate molecules with relatively easily available sources for development of anti-ASFV preparations, breaks through excessive dependence on chemical synthetic drugs in existing research and development, and broadens the research and development path of anti-ASFV drugs.

The invention relates to arecoline derivatives I-1-I-22 as well as a preparation method and application thereof in prevention and treatment of plant viruses, pathogenic bacteria and diseases. The invention provides a series of arecoline derivatives, discovers the plant virus and plant pathogen resisting activity of the compounds, and lays a foundation for synthesis and biological activity research of the compounds. The arecoline derivatives I-1-I-22 provided by the invention have anti-plant virus activity and can inhibit tobacco mosaic virus, and meanwhile, the compound shows broad-spectrum anti-plant germ activity.

The invention relates to the technical field of insect repelling fertilizers, in particular to a camphor Chinese herbal medicine insect repelling fertilizer and a preparation method and device thereof. The insect repelling fertilizer is prepared from the following raw materials in parts by weight: 18 to 22 parts of improved slow release fertilizer, 10 to 15 parts of novel cortex meliae extract, 5 to 10 parts of arecoline, 0.3 to 0.5 part of camphor, 3 to 6 parts of cnidium lactone, 10 to 14 parts of diatomite, 8 to 12 parts of amino acid chelated nitrogen, 12 to 16 parts of monocalcium phosphate, 8 to 12 parts of nano montmorillonite and 1 to 3 parts of folium artemisiae argyi volatile oil; according to the invention, multiple advantages are integrated and coordinated, a nano-carrier coating and targeted modification technology is adopted, the bioavailability and stability of active components of the cortex meliae extract are improved, the targeted repelling effect on pests is enhanced, and biological fermentation activates the soil improvement capability of potassium humate, activates soil organic matters and improves fertility; the camphor is matched with the cnidium lactone and the folium artemisiae argyi volatile oil to form an aromatic synergistic network, so that the growth of mould is inhibited, and the broad-spectrum insecticidal effect is enhanced.

The invention relates to the technical field of food preservation, in particular to a betel nut preservation method based on composite treatment.The betel nut preservation method comprises the steps that raw materials are sorted and then washed with 2 ppm ozone water, the raw materials are soaked in a color protection solution containing ascorbic acid, citric acid, phytic acid and EDTA, a chitosan-tea polyphenol-nano zinc oxide-silver composite solution is subjected to electrostatic spraying for film coating, vacuum precooling is conducted to 8 DEG C, and the betel nut is obtained. And after modified atmosphere packaging, carrying out pulsed electric field and ultrasonic cooperative treatment and 2.5 kGy gamma irradiation, and finally, intelligently storing at a constant temperature of 4 DEG C. Through cooperation of multiple technologies, the problems of microorganism exceeding, water loss and component volatilization are solved, the refreshing time reaches 4 months, the total number of bacterial colonies within 120 days is smaller than or equal to 1.5 * 10 < 3 > CFU / g, the retention rate of arecoline is larger than or equal to 92%, the weight loss rate is smaller than or equal to 4.8%, the cost is low, and large-scale production is easy.