22 results about "Arecoline" patented technology

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

The utility model relates to the technical field of betel nut Chinese patent medicine production, and discloses an extraction device for betel nut secondary alkali detection of betel nut-containing Chinese patent medicine, which comprises a bottom plate, the upper side of the bottom plate is fixedly connected with a support frame, the inside of the support frame is fixedly connected with a box body, and the lower side of the box body is fixedly connected with a purification box. The device comprises a box body, the upper side of the box body is fixedly connected with a conveying assembly, the conveying assembly is used for conveying an extracting solution, the front side of the box body is rotatably connected with a first box door, the two sides of the rear portion of the box body are fixedly connected with hydraulic rods, the output ends of the hydraulic rods are fixedly connected with a supporting plate, and the lower side of the supporting plate is fixedly connected with a brush. According to the device, impurities can be effectively removed, the filtering assembly can be conveniently cleaned, then the purity of an extract can be guaranteed, the device is provided with a refrigeration structure, and qualitative change of an extracting solution caused by the fact that the interior of the device is too hot is prevented.

The invention belongs to the technical field of disease prevention and control in aquaculture, and discloses application of arecoline derivatives in killing Bowden worms. A coarse screening experiment, a soaking toxicity test and a dose effect experiment prove that in coarse screening, 0.5 mg / L of the arecoline derivative is soaked for 90 min, so that the killing rate of the arecoline derivative on the bodworm reaches 75%, and the arecoline derivative is obviously superior to the other five kinds of arecoline and derivatives thereof such as triclabendazole and arecoline; a soaking toxicity test shows that after the arecoline derivative with the concentration of 0.7 mg / L is soaked for 90 min, the survival rate of the trachinotus ovatus is 100%, and no remarkable toxicity exists; a dose effect experiment further proves that the killing rate of 0.7 mg / L of the arecoline derivative on the boxworm is increased to 95.35%. The arecoline derivative has a remarkable effect of killing the boxworm, is high in safety to host fishes, has no obvious residual risk, can be further researched and developed to be special for preventing and controlling the boxworm in aquaculture, provides an effective scheme for solving culture diseases caused by the boxworm, and has a wide application prospect.

The present invention discloses a traditional Chinese medicine preparation for preventing and / or treating myocardial infarction, its preparation method and application, belonging to the technical field of pharmaceuticals. The traditional Chinese medicine is obtained by extracting Areca catechu with 20% - 95% ethanol. The present invention first reveals that the Areca catechu extract has an anti-myocardial infarction effect. The experimental results show that the crude extract of Areca catechu can significantly inhibit H2O2-induced cardiomyocyte death, improve the left ventricular ejection fraction of myocardial infarction mice, and reduce the myocardial infarction area, and the effect is equivalent to that of the positive drug Suxiao Jiuxin Pills, while arecoline has no cardiomyocyte protection effect. The present invention is of natural origin, simple to prepare, and low in cost, and can be used as a new supplement or alternative for clinical prevention and treatment of myocardial infarction, and has important clinical value and application prospects.

The invention relates to the field of oral disease prevention and treatment, in particular to oral ulcer gargle containing sodium surfactin, which comprises polyvinylpyrrolidone, carboxymethyl cellulose sodium, glycerol, N-acetamido glucose, sorbitol, saccharin sodium, lactic acid, sodium surfactin and purified water. The sodium bacillus subtilis lipopeptide is combined with pathogenic microorganisms and potential cancerous cells through electrostatic attraction, precise killing is achieved, PVP and CMC-Na form a physical protection layer on the ulcer surface to isolate irritants and relieve pain, sodium bacillus subtilis lipopeptide and lactic acid have a synergistic effect, cancerogenic substances such as arecoline in the oral cavity can be wrapped or degraded, the cancerous risk is reduced, and the cancerous effect is improved. And the components are physiologically inert, have good biocompatibility and do not irritate oral mucosa.

The invention discloses arecoline type triazole glycoside compounds and application thereof in preparation of weight-reducing drugs, the structural formula of the compounds is as follows: in the formula, R represents glycosyl, arecoline is used as a starting raw material, methyl is removed from carboxylic acid methyl ester group or N-methyl group, then the carboxylic acid methyl ester group or N-methyl group is converted into propyne, and the propyne is converted into propargyl; and carrying out a click reaction with an azido sugar compound to introduce different sugar units. Pharmacological activity tests show that the compound has good inhibitory activity on lipase, which indicates that the arecoline type triazole glycoside compound can be used as a novel weight-reducing drug for research and utilization.

The utility model relates to the technical field of demethylated arecoline detection, and discloses a reagent titration device for demethylated arecoline detection containing betel nut Chinese patent medicine, which comprises a bearing frame, the left side of the bearing frame is fixedly connected with a bearing plate, the upper side of the bearing plate is fixedly connected with a liquid storage cylinder, the upper side of the liquid storage cylinder is fixedly connected with a connecting assembly, and the connecting assembly is fixedly connected with the liquid storage cylinder. The connecting assembly is used for conveniently adding raw materials, a supporting frame is fixedly connected to the lower side of the bearing plate, a motor is fixedly connected to the interior of the supporting frame, a rotating shaft is fixedly connected to the output end of the motor, and a fixing sleeve is fixedly connected to the exterior of the rotating shaft; and fixing blocks which are uniformly distributed are fixedly connected to the outer part of the fixing sleeve. According to the device disclosed by the utility model, raw materials in the liquid storage cylinder can be fully and uniformly stirred, an experimenter does not need to manually shake a reagent bottle, and the dropping speed of a stock solution can be conveniently and accurately controlled to drop a standard solution.

The invention relates to application of arecoline derivatives in prevention and treatment of plant viruses and pathogenic bacteria, and finds that the arecoline derivatives I-1-I-14 show good anti-plant virus and pathogenic bacteria activity for the first time, and can well inhibit tobacco mosaic virus (TMV), cucumber fusarium wilt, peanut brown spot, apple ring rot, wheat sheath blight, tomato early blight, rice blast, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici, phytophthora capsici and phytophthora capsici, and the like. And sclerotium of rape has eight plant germs.

The invention belongs to the technical field of medicines, and discloses a composition for improving bioavailability of arecoline. Specifically, the composition specifically comprises arecoline and magnolol, and the weight ratio of arecoline to magnolol in the pharmaceutical composition is (1: 0.1)-(1: 15). According to the composition, the in-vivo bioavailability of the arecoline can be remarkably improved, the half-life period and the average residence time of the arecoline can be prolonged, the application range of the arecoline is widened, and the treatment effect of the arecoline on diseases such as tapeworm infection, abdominal distension, diarrhea, edema, cancer, Alzheimer's disease, cerebral apoplexy, epilepsy and viral pneumonia can be better exerted.

The invention discloses a preparation method of betel nut powder. The preparation method comprises the following steps: adding water into fresh betel nut freeze-dried powder, and carrying out enzymolysis for 150-200 minutes by using a compound enzyme under the conditions that the pH value is 5.0-5.5 and the temperature is 40-45 DEG C; adding a carrier, sodium bicarbonate, lecithin and coconut oil into the obtained enzymatic hydrolysate, and homogenizing and emulsifying at the high pressure of 45-50MPa; and carrying out spray drying under the conditions that the air inlet temperature is 110-120 DEG C, the air outlet temperature is 60-65 DEG C and the rotating speed of an atomizer is 20000-25000rpm, and crushing to obtain powder with the particle size of less than 100nm. The invention further provides the prepared areca nut powder and candies containing the areca nut powder. According to the preparation method, loss of effective components such as arecoline in the preparation process is prevented. The prepared areca nut powder is high in content of active ingredients such as arecoline and the like, and is free of bitterness and astringent taste.

This application provides a composition and a product thereof. The composition includes arecoline and an acid. The composition is convenient to use and widely used, with a low pH value and weak irritation.

The invention discloses a method for detecting arecoline residues in a biological sample and application of the method. According to the method, a high performance liquid chromatography-tandem mass spectrometry method is used for testing and analyzing, an octadecyl silane bonded silica gel chromatographic column is used as a detection chromatographic column, 0.1% formic acid water (A)-10mmol / L ammonium formate water (B) is used as a mobile phase, a gradient elution procedure is adopted, and specific mass spectrometry conditions are matched, so that the arecoline in a biological sample can be quantitatively determined. The detection method disclosed by the invention is applied to the detection research of the arecoline residues in the biological tissues, and fills the current technical blank in the aspect of detecting the arecoline residues in the biological samples, especially in the chicken edible tissues.

The invention provides arecoline hydrazide derivatives as well as preparation and application thereof in preparation of anti-inflammatory drugs, and relates to the technical field of drug compound synthesis. The arecoline hydrazide derivative has a structural formula as shown in the following formula (1), wherein R is phenyl or substituted phenyl. According to the invention, arecoline is used as a raw material, an ester group in arecoline is hydrolyzed to generate an arecoline intermediate, and arecoline carboxyl is further catalyzed to be subjected to a condensation reaction with various substituted hydrazine to obtain the arecoline hydrazide compound. The arecoline hydrazide derivatives of the series can remarkably inhibit release of an inflammatory medium NO in RAW264.7 cells induced by LPS and the expression level of inflammatory cytokines TNF-alpha and IL-6 protein; the compound has high in-vitro anti-inflammatory activity and low cytotoxicity, and can be used for preparing anti-inflammatory drugs.

This invention utilizes *Allium macrostemon* to obtain its aqueous extract, petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract. The effects of these aqueous extracts and extracts of different polarities on the learning and memory functions of D-galactose-induced aging model mice were investigated using Y-maze, darkness avoidance, Morris water maze, and laser speckle cerebral blood flow assays. Reactive oxygen species (ROS), SOD, and MDA were also measured. Multiple experiments demonstrated that *Allium macrostemon* can improve memory function in mice. Furthermore, the effects of *Allium macrostemon* extract on arecoline- or oxidative tremor-induced tremor behavior in mice were studied. The results showed that *Allium macrostemon* extract can effectively shorten the duration of tremors, proposing a new application for *Allium macrostemon* extract in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases, providing broad application prospects for the treatment or adjuvant therapy of neurodegenerative diseases.

The invention relates to the technical field of arecoline application and particularly discloses application of arecoline in inhibition of African swine fever virus replication and proliferation and a method thereof, the arecoline is applied to an antiviral preparation, the concentration of the arecoline acting on porcine PK cells in the preparation is 400 micromol / L, the acting time is 24 h, the concentration of the arecoline acting on the porcine PK cells in the preparation is 400 micromol / L, and the concentration of the arecoline acting on the porcine PK cells in the preparation is The expression of the preparation for inhibiting the replication and proliferation of the African swine fever virus comprises reduction of transcription and expression levels of virus specific genes, and the virus specific genes comprise a P30 gene, a P54 gene and a P72 gene. The discovery fills the key blank of anti-ASFV research of natural compounds, provides brand-new natural candidate molecules with relatively easily available sources for development of anti-ASFV preparations, breaks through excessive dependence on chemical synthetic drugs in existing research and development, and broadens the research and development path of anti-ASFV drugs.

The invention relates to arecoline derivatives I-1-I-22 as well as a preparation method and application thereof in prevention and treatment of plant viruses, pathogenic bacteria and diseases. The invention provides a series of arecoline derivatives, discovers the plant virus and plant pathogen resisting activity of the compounds, and lays a foundation for synthesis and biological activity research of the compounds. The arecoline derivatives I-1-I-22 provided by the invention have anti-plant virus activity and can inhibit tobacco mosaic virus, and meanwhile, the compound shows broad-spectrum anti-plant germ activity.

The invention relates to the technical field of insect repelling fertilizers, in particular to a camphor Chinese herbal medicine insect repelling fertilizer and a preparation method and device thereof. The insect repelling fertilizer is prepared from the following raw materials in parts by weight: 18 to 22 parts of improved slow release fertilizer, 10 to 15 parts of novel cortex meliae extract, 5 to 10 parts of arecoline, 0.3 to 0.5 part of camphor, 3 to 6 parts of cnidium lactone, 10 to 14 parts of diatomite, 8 to 12 parts of amino acid chelated nitrogen, 12 to 16 parts of monocalcium phosphate, 8 to 12 parts of nano montmorillonite and 1 to 3 parts of folium artemisiae argyi volatile oil; according to the invention, multiple advantages are integrated and coordinated, a nano-carrier coating and targeted modification technology is adopted, the bioavailability and stability of active components of the cortex meliae extract are improved, the targeted repelling effect on pests is enhanced, and biological fermentation activates the soil improvement capability of potassium humate, activates soil organic matters and improves fertility; the camphor is matched with the cnidium lactone and the folium artemisiae argyi volatile oil to form an aromatic synergistic network, so that the growth of mould is inhibited, and the broad-spectrum insecticidal effect is enhanced.

The invention belongs to the technical field of composite film agents, and relates to a nicotine and arecoline three-layer composite oral soluble film and a preparation method thereof, and the nicotine and arecoline three-layer composite oral soluble film sequentially comprises a lower polymer film layer containing arecoline, a middle polymer film layer containing nicotine and an upper polymer film layer containing arecoline from bottom to top. Respectively preparing an upper-layer glue solution, a lower-layer glue solution and a middle-layer glue solution, casting the lower-layer glue solution on a substrate, and drying to form a lower-layer film; casting the middle-layer glue solution on the dried lower-layer film, and drying to obtain a double-layer film; and finally, casting the upper-layer glue solution on the dried double-layer film, drying to obtain a three-layer composite oral soluble film, and slitting into oral soluble membranes with preset sizes. Through the design of the three-layer structure, the sequential release of the active components is realized, the arecoline and nicotine are physically isolated in different film layers, the arecoline and nicotine have a synergistic effect and mutually cover the defect of independent use, and the release stability and the use safety of the product are improved.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.

The application discloses an arecoline derivative containing an amino acid fragment, a preparation method of the arecoline derivative, and application of the arecoline derivative as an agricultural fungicide. The arecoline derivative containing the amino acid fragment has a novel molecular structure and excellent inhibitory activity on plant pathogenic bacteria, is suitable for prevention and treatment of agricultural plant pathogenic bacteria, can be used for development and use of effective components of agricultural fungicides or adjuvants, and has the potential of development and application as a new agricultural fungicide.

This invention discloses an enantioselective imine reductase mutant and its application in the production of S-arecoline, belonging to the field of enzyme engineering technology. The imine reductase mutant is obtained by single- or multi-point mutation of the substrate-binding pocket key amino acid site of wild-type imine reductase derived from *Pseudomonas* sp. RC4D1. This mutant can efficiently catalyze the asymmetric reduction of pseudo-arecoline, maintaining high enzyme activity while increasing the optical purity of the product S-arecoline to 99.9%. Combined with a glucose dehydrogenase coenzyme cycle system, it significantly reduces production costs. The reaction conditions are mild and environmentally friendly, enabling the industrial-scale production of S-arecoline and providing a high-purity raw material for fiber-free areca chewing gum.

The application discloses a multi-layer embedding areca alkaloid sustained-release system and a preparation method and application thereof, relates to the technical field of areca alkaloid, and the sustained-release system comprises an areca alkaloid core layer, an antioxidation layer and a protective layer. The areca alkaloid sustained-release core layer is located in the innermost layer, the antioxidation layer is wrapped outside the sustained-release core layer, and the protective layer is wrapped outside the antioxidation layer. The areca alkaloid is wrapped by the sustained-release core layer, the antioxidation layer and the protective layer in sequence, can effectively resist the damage of gastric acid and other environmental conditions to the structure of the areca alkaloid, prevents the oxidation inactivation of the areca alkaloid, realizes the target of targeting the intestinal tract of the active areca alkaloid, and meanwhile, can avoid the contact of the areca alkaloid with other organ tissues, and reduces the side effects of the areca alkaloid.