14 results about "Phytophthora capsici" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The application relates to the technical field of plant disease detection, and discloses a primer for detecting pepper Phytophthora capsici, application of the primer, and a product and method for detecting pepper Phytophthora capsici. A high-specificity amplification primer is designed according to a specific sequence of a genome of pepper Phytophthora capsici, the primer can avoid interference of multiple related fungi, and can amplify a sufficient target for detection under a condition of low-abundance pathogenic bacteria; meanwhile, the provided amplification primer is combined with a CRISPR / Cas12a technology, rapid and accurate detection and identification of pepper Phytophthora capsici can be realized, and a very high sensitivity is achieved. In a limited environment, the detection system provided by the application is not only high in sensitivity and strong in specificity, but also short in time consumption and convenient in operation, and provides an effective tool for rapid identification of pepper Phytophthora capsici.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

This invention discloses a berberine hydrochloride-resveratrol self-assembled nanopesticide, its preparation method, and its application. The preparation method involves dissolving berberine hydrochloride and resveratrol separately in an organic solvent, mixing them in a molar ratio of 3:1–1:3, adding the mixture to deionized water, and then adding an alkaline solution; the mixture then self-assembles to obtain nanoparticles. Based on the principle of supramolecular construction, this invention utilizes the high compatibility of berberine hydrochloride and resveratrol in charge distribution and spatial geometry. Through multiple intermolecular forces such as electrostatic attraction, π-π stacking, and hydrogen bonding, the precise recognition and directional assembly of the two active molecules in the aqueous phase are achieved. This nanopesticide requires no additional synthetic adjuvants or nanocarriers, achieving confined enrichment of the active ingredients at the nanoscale, resulting in a significant synergistic effect. This nanopesticide significantly enhances the inhibitory activity against various pathogens such as *Botrytis cinerea*, *Fusarium oxysporum*, *Phytophthora capsici*, and *Rhizoctonia solani*, and exhibits excellent environmental biosafety.

This invention discloses the effector protein *PpiniAvh17* from *Phytophthora indicum*, its encoding gene, and its applications, belonging to the field of molecular biology. The amino acid sequence of this protein is shown in SEQ ID NO.1. Experiments have demonstrated that this protein can effectively inhibit programmed cell death (PCD) induced by pathogen effector proteins (such as *Phytophthora indicum* INF1) and can promote *Phytophthora capsici* infection in plants, indicating that it functions as a toxic effector to suppress plant immunity. This invention also provides the gene encoding this protein (SEQ ID NO.2). This protein and its encoding gene can be used to prepare products that regulate plant cell death, enhance plant stress resistance, or study plant-pathogen interaction mechanisms, providing new molecular targets and tools for plant disease control.

The present application relates to the application of spermine and / or spermidine in preventing and treating plant diseases caused by Phytophthora. The present application finds that spermine and spermidine can effectively inhibit the spore germination and mycelium growth of Phytophthora capsici Leonian and P. infestans Mont. and can effectively prevent and treat the plant diseases caused by P. capsici Leonian and P. infestans Mont., including pepper Phytophthora blight, tomato Phytophthora blight, potato late blight and tomato late blight, and significantly reduce the plant disease area.

This invention belongs to the fields of organic synthesis and pesticide application, and specifically relates to the application of an indole / indazole compound with an imine fragment, the structural formula of which is as follows: This invention tested the indole / indazole compounds with the imine fragment in vitro against *P. capsici*, *P. aphaneridermatum*, and *P. litchii*. It was found that compounds A1 and A5 exhibited excellent activity against oomycetes, particularly against *P. capsici*, with EC50 values ​​of 4.4 μg / mL and 2.6 μg / mL, respectively, similar to the positive control metalaxyl (2.3 μg / mL). It is also noteworthy that when compounds A1-A5 are prepared as Cu... 2+ When the ionic complex B1-B5 was used, its inhibitory effect on oomycetes was significantly enhanced. Complex B1 showed excellent in vivo protective and curative effects against Phytophthora capsici on pepper leaves, with control efficiencies of 91.45% and 83.75% at a concentration of 200 μg / mL, respectively, which were superior to metalaxyl (80.05% and 76.15%).

This invention reveals a transparent nanoemulsion biopesticide formulation of citronella oil with a particle size of less than 50 nm that functions as a biopesticide. A nano-biopesticide formulation based on citronella oil consisting of (1) citronella oil 5-8%, (2) emulsifier tween 1-2.4%, (3) turpentine oil 0.5-1%, (4) wetting agent Ethylan@SP25 8-10%, (5) glycerol 1-1.6%, and the remainder is distilled water, the total percentage of the ingredients being 100%, which is characterized by a clear / transparent mixture with a particle size of less than 50 nm. This invention functions as a biopesticide to control the fungus Phytophthora capsici and pests of aphids, thrips, and mites on chili plants.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

This invention discloses a class of acetylhydrazine derivatives with phenyl heteroatom substituents, their preparation methods, and applications; the acetylhydrazine derivatives have the general structural formula shown below, and exhibit excellent broad-spectrum antifungal effects against fungi including *Fusarium graminearum*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Rhizoctonia solani*, *Alternaria alternata*, *Curvularia lunata*, *Phytophthora capsici*, *Sclerotium sclerotiorum*, and *Fusarium graminearum*.

This invention proposes a screening method for biocontrol Trichoderma strains based on the dual criteria of oomycete parasitism and lethality. Belonging to the field of agricultural biotechnology, this invention addresses the technical problem that existing screening techniques often rely solely on the growth inhibition rate of confrontation cultures, making it difficult to distinguish between the antibacterial and lethal effects of biocontrol bacteria. This invention establishes a progressive screening system. The method uses highly pathogenic oomycetes, namely *Phytophthora capsici* (… Phytophthora capsici ) and ultimate pyrophyllium ( Globisporangium ultimum Using oomycetes as the target, and based on the determination of growth inhibition rate through confrontation culture, this invention creatively introduces a step of "pathogen re-isolation and detection of activity based on oomycete selective culture medium" combined with microscopic observation of hyperparasitism. This method effectively eliminates false-positive strains, significantly improves the accuracy of biocontrol strain screening and field efficacy, and is suitable for large-scale exploration of biocontrol resources.