79 results about "Phytophthora capsici" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses a litchi disease-resistant gene LcEXP1 and an application thereof. The litchi disease-resistant gene LcEXP1 is separated from litchis, the full-length cDNA (complementary deoxyribonucleic acid) of the gene is 750bp, and the encoded protein is an expansion protein with the full length consisting of 250 amino acids. The litchi disease resistance related gene LcEXP1 is transiently expressed in tobacco, different tobacco leaves are inoculated with phytophthora capsici and alternaria alternata after two days, it can be observed that after expression of the LcEXP1, scabs are remarkably reduced compared with negative control, and biostatistical analysis shows that the areas of the two treated scabs are remarkably different. Therefore, the gene can effectively promote the disease resistance of plants such as tobacco and the like, and has a wide application prospect in developing products for improving the disease resistance of the plants.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses a seed priming method for improving the early disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds in sodium hypochlorite and then washing with sterile deionized water; S2, pulse phosphorylation activation treatment: adding seeds and activation solution to a pressure-resistant polypropylene container connected to a vacuum pipeline, evacuating to 0.08 MPa after sealing, returning to normal pressure after maintaining for 5-10 minutes, and then transferring to a constant temperature oscillator for phosphorylation activation treatment; S3, immune training treatment: transferring the seeds after phosphorylation activation treatment to a priming solution containing a suspension of UV-inactivated Phytophthora capsici spores and H2O2, and intermittent ventilation treatment; S4, stabilization treatment: transferring the seeds after immune training treatment to an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain pepper seeds. The present invention solves the problem of immune response interruption caused by insufficient signal intensity and rapid protein turnover when stimulating using a simulated environment in the prior art.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.

The invention belongs to the field of marine natural product chemistry, and particularly relates to marine fungi and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. 13 monomeric compounds are separated and identified, Territrem B shows a remarkable antifungal effect on phytophthora capsici, the EC50 value is 22.96 mu g / mL, mycelial growth, sporangium formation and zoospore generation of the phytophthora capsici can be effectively inhibited, and the defense capacity of pepper plants is enhanced; the form of the phytophthora capsici mycelium is changed, and the integrity of a cell membrane is destroyed. In addition, Territrem B inhibits the activity of antioxidant enzyme, reduces the content of soluble protein and increases the MDA level, thereby inhibiting the normal growth of phytophthora capsici.

The application relates to the technical field of plant disease detection, and discloses a primer for detecting pepper Phytophthora capsici, application of the primer, and a product and method for detecting pepper Phytophthora capsici. A high-specificity amplification primer is designed according to a specific sequence of a genome of pepper Phytophthora capsici, the primer can avoid interference of multiple related fungi, and can amplify a sufficient target for detection under a condition of low-abundance pathogenic bacteria; meanwhile, the provided amplification primer is combined with a CRISPR / Cas12a technology, rapid and accurate detection and identification of pepper Phytophthora capsici can be realized, and a very high sensitivity is achieved. In a limited environment, the detection system provided by the application is not only high in sensitivity and strong in specificity, but also short in time consumption and convenient in operation, and provides an effective tool for rapid identification of pepper Phytophthora capsici.

The invention provides an anti-continuous cropping bacterial fertilizer based on bacillus velezensis and application of the anti-continuous cropping bacterial fertilizer. According to the invention, firstly, a strain of bacillus velezensis zhanshen-K1 is separated from soil for continuous cropping of hot peppers, and the preservation number of the strain of bacillus velezensis zhanshen-K1 is CGMCC (China General Microbiological Culture Collection Center) No.34459. The strain has the effects of fixing nitrogen, dissolving potassium, decomposing protein, producing exopolysaccharides, antagonizing various pathogenic bacteria such as fusarium fusiforme, fusarium asian, alternaria alternata and phytophthora capsici, degrading various phenolic acid autotoxic substances such as benzoic acid, p-hydroxybenzoic acid and cinnamic acid, and the like; and meanwhile, the continuous cropping-resistant bacterial fertilizer is prepared by adding wormcast and sodium humate into the compound serving as a main effective component, so that continuous cropping obstacles of crops can be reduced.

The application belongs to the field of biological control of plant diseases, and specifically discloses a biocontrol bacterium Bacillus velezensis and application of the biocontrol bacterium in preparation of a biocontrol bacterium agent for preventing and treating pepper blight. Bacillus velezensis The strain is Bacillus velezensis (Bacillus velezensis) Y426-7, which was preserved in the China General Microbiological Culture Collection Center on September 23, 2024, has a preservation number of CGMCC NO.32041, and is located at No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The biocontrol bacterium Bacillus velezensis can be used to prepare the biocontrol bacterium agent for preventing and treating pepper blight. Compared with the prior art, the Bacillus velezensis Y426-7 obtained by the application has obvious bacteriostatic effect on pepper Phytophthora capsici Leonian and various crop pathogenic fungi under indoor in vitro conditions; and 2.5x10 9 CFU / g wettable powder prepared from the Bacillus velezensis Y426-7 has good prevention and treatment effect and certain yield-increasing effect on pepper blight in a field.

The invention discloses a pseudomonas strain JQ36 and a preparation method and application of a peptide compound produced by the pseudomonas strain JQ36. The pseudomonas JQ36 is separated from rhizosphere soil of wheat in Gansu province, and peptide substances with novel structures can be generated through liquid fermentation. The substances can be separated and purified by combining a solid-phase extraction technology with a high performance liquid chromatography technology to obtain monomeric compounds. Under in-vitro experiment conditions, the peptide substance can inhibit the zoospore germination of phytophthora capsici and split the zoospore of phytophthora capsici; in a pepper living body, the compound with the concentration of 50 mu M can well prevent and control phytophthora capsici diseases, and compared with a control group, the compound with the concentration can achieve the phytophthora capsici prevention and control effect of 83% or above. Therefore, the compound with the novel structure has a good phytophthora capsici prevention and control effect, can be used for preparing biological medicines for preventing and controlling phytophthora capsici, and has an important potential application value in the fields of agricultural production and the like.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The invention belongs to the technical field of plant disease and insect pest prevention and control, and particularly relates to application of nerol in prevention and control of rubber anthracnose. It is found for the first time that nerol with low concentration (0.5 [mu] L / mL) can effectively inhibit colletotrichum gloeosporioides, phytophthora capsici and colletotrichum gloeosporioides, and especially the inhibition rate of nerol to colletotrichum gloeosporioides and colletotrichum gloeosporioides can reach 100%. Besides, nerol also has obvious inhibitory activity on rubber anthrax bacteria inoculated on in-vitro leaves, and can obviously reduce the area of scabs, and the inhibition rate reaches 98.4%. The results show that the nerol has a good prevention and treatment effect on the rubber anthracnose, the nerol belongs to a plant-sourced volatile compound, and few residues are left when the nerol is used for preventing and treating the rubber tree anthracnose, so that the nerol can be used as a potential green pesticide for preventing and treating the rubber anthracnose, and a new way is provided for preventing and treating the rubber anthracnose.

The invention discloses a fungus strain 41233 from Western sand and application of the fungus strain 41233, the taxonomic name of the fungus strain 41233 is Plectosphaerella sp., the preservation number of the fungus strain 41233 is GDMCC No.67505, and the preservation date of the fungus strain 41233 is December 19, 2025. The invention further discloses application of the fungus strain 41233 and a crude extract of the fungus strain 41233 in preparation of pathogenic bacterium inhibition products. The fungus strain 41233 sourced from Xiaxa has inhibitory activity on mango colletotrichum gloeosporioides and / or phytophthora capsici, and a crude extract of the fungus strain 41233 sourced from Xiaxa has inhibitory activity on various pathogenic bacteria such as colletotrichum gloeosporioides, phytophthora and fruit rot pathogens, and can be used for preparing biocontrol inoculants or further mining active natural products. And a new green prevention and control means is provided for plant disease prevention and control.

The invention discloses a pesticide composition containing chloromethylsilazane and application of the pesticide composition in plant disease prevention and growth promotion, and belongs to the technical field of biocide pesticides. According to the invention, the red date kernel extract and the digitaria sanguinalis extract are taken as synergistic extracts and are combined with the chloromethylsilatrane and the 14-hydroxy brassin to prepare the pesticide composition containing the chloromethylsilatrane. When the phytophthora capsici is applied to a test, in a pepper disease resistance test, the disease index is reduced to some extent, and the relative control effect is up to 79.45% at most, which indicates that the phytophthora capsici is inhibited; in the experiment of promoting the growth of the pepper, the plant height of the pepper plant in the compound group is 19.67 + / -0.23 cm, the stem diameter is 4.07 + / -0.05 mm, the dry weight of the overground part is 1.51 + / -0.11 g, the dry weight of the underground part is 0.39 + / -0.04 g, and the growth index is remarkably improved. The pesticide composition containing the chloromethylsilazane prepared by the invention can better inhibit phytophthora capsici, is suitable for developing pesticides for preventing and treating phytophthora capsici, and also has a better promoting effect on the growth of capsicum.

The invention relates to application of bisindole alkaloid iheyamine A and derivatives thereof in treatment of plant virus pathogens, and finds that iheyamine A and derivatives I-1-I-15 thereof show good anti-plant virus pathogen activity for the first time, and can well inhibit tobacco mosaic virus (TMV), cucumber fusarium wilt, peanut brown spot, apple ring rot, wheat sheath blight, tomato early blight and rice blast. , phytophthora capsici and sclerotium of rape.

This invention discloses a berberine hydrochloride-resveratrol self-assembled nanopesticide, its preparation method, and its application. The preparation method involves dissolving berberine hydrochloride and resveratrol separately in an organic solvent, mixing them in a molar ratio of 3:1–1:3, adding the mixture to deionized water, and then adding an alkaline solution; the mixture then self-assembles to obtain nanoparticles. Based on the principle of supramolecular construction, this invention utilizes the high compatibility of berberine hydrochloride and resveratrol in charge distribution and spatial geometry. Through multiple intermolecular forces such as electrostatic attraction, π-π stacking, and hydrogen bonding, the precise recognition and directional assembly of the two active molecules in the aqueous phase are achieved. This nanopesticide requires no additional synthetic adjuvants or nanocarriers, achieving confined enrichment of the active ingredients at the nanoscale, resulting in a significant synergistic effect. This nanopesticide significantly enhances the inhibitory activity against various pathogens such as *Botrytis cinerea*, *Fusarium oxysporum*, *Phytophthora capsici*, and *Rhizoctonia solani*, and exhibits excellent environmental biosafety.