96 results about "Phytophthora capsici" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.

The invention discloses a Trichoderma auritum GUWM22 strain and application thereof, and belongs to the field of microorganisms. The preservation number of the trichoderma auritum GUWM22 is CCTCC (China Center for Type Culture Collection) NO: M20242639, the preservation date is November 26, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. Experiments prove that the GUWM22 has a very strong inhibition effect on six pathogenic bacteria (phytophthora nicotianae, phytophthora capsici, botryosphaeria dothidea, alternaria alternata, phomopsis and colletotrichum capsici), the bacteriostasis rate reaches 78% or above, in addition, the GUWM22 also has a good prevention and treatment effect on anthracnose of picked peppers (line peppers), the prevention effect is 82.11%, and it can be seen that the GUWM22 can be used for preventing and treating anthracnose of the picked peppers (line peppers). The Trichoderma aureum GUWM22 is a new biocontrol Trichoderma strain with good potential. The invention provides a new strain resource and an environment-friendly and efficient biocontrol approach for effective prevention and treatment of pepper anthracnose.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses a litchi disease-resistant gene LcEXP1 and an application thereof. The litchi disease-resistant gene LcEXP1 is separated from litchis, the full-length cDNA (complementary deoxyribonucleic acid) of the gene is 750bp, and the encoded protein is an expansion protein with the full length consisting of 250 amino acids. The litchi disease resistance related gene LcEXP1 is transiently expressed in tobacco, different tobacco leaves are inoculated with phytophthora capsici and alternaria alternata after two days, it can be observed that after expression of the LcEXP1, scabs are remarkably reduced compared with negative control, and biostatistical analysis shows that the areas of the two treated scabs are remarkably different. Therefore, the gene can effectively promote the disease resistance of plants such as tobacco and the like, and has a wide application prospect in developing products for improving the disease resistance of the plants.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention provides a streptomyces hygroscopicus engineering strain as well as construction and application thereof, and relates to the technical field of biology, the engineering strain takes streptomyces hygroscopicus CL-1 as a starting host and is obtained by heterologous expression of an epsilon-polylysine synthetase encoding gene pls, and the nucleotide sequence of the epsilon-polylysine synthetase encoding gene pls is shown as SEQ ID NO.1. Streptomyces hygroscopicus CL-1 is used as a host, the epsilon-polylysine synthetase coding gene derived from streptomyces albus is subjected to heterologous expression, the obtained recombinant strain has high epsilon-polylysine production capacity, and under the optimized fermentation condition, the yield of epsilon-polylysine is 0.87 g / L, and industrial scale production requirements are met. Meanwhile, the broad-spectrum antibacterial activity to various plant pathogenic fungi and common bacteria, including colletotrichum gloeosporioides, fusarium oxysporum, phytophthora capsici and the like, is also remarkably improved. Therefore, the streptomyces hygroscopicus has important application in the aspects of production of epsilon-polylysine, prevention and control of pathogenic microorganisms, preservation and the like, and the industrial and agricultural utilization potential of the streptomyces hygroscopicus is expanded.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses a seed priming method for improving the early disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds in sodium hypochlorite and then washing with sterile deionized water; S2, pulse phosphorylation activation treatment: adding seeds and activation solution to a pressure-resistant polypropylene container connected to a vacuum pipeline, evacuating to 0.08 MPa after sealing, returning to normal pressure after maintaining for 5-10 minutes, and then transferring to a constant temperature oscillator for phosphorylation activation treatment; S3, immune training treatment: transferring the seeds after phosphorylation activation treatment to a priming solution containing a suspension of UV-inactivated Phytophthora capsici spores and H2O2, and intermittent ventilation treatment; S4, stabilization treatment: transferring the seeds after immune training treatment to an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain pepper seeds. The present invention solves the problem of immune response interruption caused by insufficient signal intensity and rapid protein turnover when stimulating using a simulated environment in the prior art.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The invention discloses a dehydroabietyl quaternary ammonium salt of which the chemical structural formula is shown in the following formula. The dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani, and the dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani. The method has potential value in preparation of green pesticide bactericides, and can reduce adverse effects of the bactericides on the environment and human beings; # imgabs0 #

The present invention discloses a benzamide derivative containing a thiocyanatopyrazole structure, a preparation method thereof and an application thereof. The structural formula of this benzamide derivative containing a thiocyanatopyrazole structure is as follows in general formula (I), #imgabs0# wherein, R1 is trifluoromethyl, difluoromethyl or methyl; R2 is hydrogen, methyl, methoxy, trifluoromethyl, trifluoromethoxy, fluorine, chlorine, bromine, cyano or a multi-substituted combination thereof substituted at different positions. The benzamide derivative containing a thiocyanatopyrazole structure prepared by the present invention has broad-spectrum antifungal and oomycete activities, and particularly shows significant inhibitory activities against Valsa mali and Phytophthora capsici, which provides a basis for the development of a novel fungicide with the benzamide derivative containing a thiocyanatopyrazole structure as an active ingredient.

The invention relates to a strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 and an application of the strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 in prevention and treatment of plant oomycetes diseases, and belongs to the field of research and development of microbial pesticides. The Brevibacillus formis LJQJ21 has the capabilities of promoting growth and preventing diseases, has a relatively strong inhibition effect on mycelial growth of various pathogenic oomycetes (phytophthora capsici, phytophthora infestans, peronophythora litchii and pythium aphanidermatum) causing crop diseases, and can secrete pentapeptide antibacterial substances Edeine A and B, and the Edeine can remarkably inhibit germination of zoospores of the phytophthora capsici. In addition, the microbial agent taking the LJQJ21 spore fermentation bacterial liquid as a main active component has a relatively good prevention and treatment effect on fruit rot caused by phytophthora capsici. The Brevibacillus forts LJQJ21 has obvious growth promoting and disease preventing effects, is safe to human and livestock and environment-friendly, and has potential field application value and development prospect.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.

The invention belongs to the field of marine natural product chemistry, and particularly relates to marine fungi and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. 13 monomeric compounds are separated and identified, Territrem B shows a remarkable antifungal effect on phytophthora capsici, the EC50 value is 22.96 mu g / mL, mycelial growth, sporangium formation and zoospore generation of the phytophthora capsici can be effectively inhibited, and the defense capacity of pepper plants is enhanced; the form of the phytophthora capsici mycelium is changed, and the integrity of a cell membrane is destroyed. In addition, Territrem B inhibits the activity of antioxidant enzyme, reduces the content of soluble protein and increases the MDA level, thereby inhibiting the normal growth of phytophthora capsici.

The application relates to the technical field of plant disease detection, and discloses a primer for detecting pepper Phytophthora capsici, application of the primer, and a product and method for detecting pepper Phytophthora capsici. A high-specificity amplification primer is designed according to a specific sequence of a genome of pepper Phytophthora capsici, the primer can avoid interference of multiple related fungi, and can amplify a sufficient target for detection under a condition of low-abundance pathogenic bacteria; meanwhile, the provided amplification primer is combined with a CRISPR / Cas12a technology, rapid and accurate detection and identification of pepper Phytophthora capsici can be realized, and a very high sensitivity is achieved. In a limited environment, the detection system provided by the application is not only high in sensitivity and strong in specificity, but also short in time consumption and convenient in operation, and provides an effective tool for rapid identification of pepper Phytophthora capsici.

The invention provides an anti-continuous cropping bacterial fertilizer based on bacillus velezensis and application of the anti-continuous cropping bacterial fertilizer. According to the invention, firstly, a strain of bacillus velezensis zhanshen-K1 is separated from soil for continuous cropping of hot peppers, and the preservation number of the strain of bacillus velezensis zhanshen-K1 is CGMCC (China General Microbiological Culture Collection Center) No.34459. The strain has the effects of fixing nitrogen, dissolving potassium, decomposing protein, producing exopolysaccharides, antagonizing various pathogenic bacteria such as fusarium fusiforme, fusarium asian, alternaria alternata and phytophthora capsici, degrading various phenolic acid autotoxic substances such as benzoic acid, p-hydroxybenzoic acid and cinnamic acid, and the like; and meanwhile, the continuous cropping-resistant bacterial fertilizer is prepared by adding wormcast and sodium humate into the compound serving as a main effective component, so that continuous cropping obstacles of crops can be reduced.

The application belongs to the field of biological control of plant diseases, and specifically discloses a biocontrol bacterium Bacillus velezensis and application of the biocontrol bacterium in preparation of a biocontrol bacterium agent for preventing and treating pepper blight. Bacillus velezensis The strain is Bacillus velezensis (Bacillus velezensis) Y426-7, which was preserved in the China General Microbiological Culture Collection Center on September 23, 2024, has a preservation number of CGMCC NO.32041, and is located at No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The biocontrol bacterium Bacillus velezensis can be used to prepare the biocontrol bacterium agent for preventing and treating pepper blight. Compared with the prior art, the Bacillus velezensis Y426-7 obtained by the application has obvious bacteriostatic effect on pepper Phytophthora capsici Leonian and various crop pathogenic fungi under indoor in vitro conditions; and 2.5x10 9 CFU / g wettable powder prepared from the Bacillus velezensis Y426-7 has good prevention and treatment effect and certain yield-increasing effect on pepper blight in a field.