127 results about "Phytophthora capsici" patented technology

The invention provides bacillus velezensis NJ157 and application thereof, and relates to the technical field of biological pesticides. The bacillus velezensis NJ157 can resist saline and alkaline, has a good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum, fusarium moniliforme and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus velezensis NJ157 disclosed by the invention also has relatively strong capability of producing protease, cellulase and IAA (Indoleacetic Acid), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention provides bacillus paralicheniformis NJ102 and application thereof, and belongs to the technical field of microorganisms. The bacillus paralicheniformis NJ102 disclosed by the invention has the capabilities of resisting low temperature and saline and alkaline, and can endure the low temperature of 10 DEG C, the salt stress with the salt content of 17% and the strong alkali stress with the pH value of 12.5. Meanwhile, the bactericidal composition has a relatively good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus paralicheniformis disclosed by the invention also has relatively strong capability of producing protease, cellulase, amylase and IAA (Indoleacetic Acid), has a relatively good growth promoting effect on tomatoes, and has great application potential in the aspect of biopesticide.

The invention discloses a phytophthora plant pathogenic oomycete histone lysine methyl transfer protein as well as a coding gene and application thereof. Wherein the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification, and the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification; the coding gene is shown as a sequence 1. Experiments prove that the KMT3-1 protein plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth rate of phytophthora hyphae is slowed down, the sporangium yield is remarkably reduced, the release of zoospores is stopped, the oospore yield is reduced, the pathogenicity is reduced, and the like. Corresponding histone methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed on the basis of the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention belongs to the field of biological prevention and control of plant diseases, and particularly discloses a biocontrol bacterium bacillus velezensis and application thereof in preparation of a biocontrol inoculant for preventing and controlling pepper phytophthora blight, the strain is bacillus velezensis Y426-7, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, and has the preservation number of CGMCC NO.32041, and the preservation number is CGMCC NO.32041. The biocontrol bacterium is bacillus velezensis Y426-7. The bacillus velezensis is preserved in No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and can be used for preparing a biocontrol inoculant for preventing and treating pepper phytophthora blight. Compared with the prior art, the bacillus velezensis Y426-7 obtained by the invention has an obvious antibacterial effect on phytophthora capsici and various crop phytophthora blight bacteria under an indoor in-vitro condition; furthermore, the 2.5 * 10 < 9 > CFU / g wettable powder prepared from the bacillus velezensis Y426-7 has a relatively good prevention and treatment effect and a certain yield increasing effect on the pepper phytophthora blight in the field.

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.

The invention discloses arginine methyltransferase protein from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The arginine methyltransferase protein provided by the invention is as shown in sequences 6-8; the coding gene is shown in sequences 1-3. Experiments prove that the protein disclosed by the invention plays an important role in growth, development and pathopoiesis processes of typical phytophthora plant pathogen oomycete phytophthora capsici, and after the protein is deleted or the expression level is reduced, the growth rate of phytophthora capsici hyphae is reduced, the sporangium yield is reduced, the number of zoospore is reduced, the pathogenicity is reduced and the like. Corresponding arginine methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora capsici, phytophthora sojae and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses a Trichoderma auritum GUWM22 strain and application thereof, and belongs to the field of microorganisms. The preservation number of the trichoderma auritum GUWM22 is CCTCC (China Center for Type Culture Collection) NO: M20242639, the preservation date is November 26, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. Experiments prove that the GUWM22 has a very strong inhibition effect on six pathogenic bacteria (phytophthora nicotianae, phytophthora capsici, botryosphaeria dothidea, alternaria alternata, phomopsis and colletotrichum capsici), the bacteriostasis rate reaches 78% or above, in addition, the GUWM22 also has a good prevention and treatment effect on anthracnose of picked peppers (line peppers), the prevention effect is 82.11%, and it can be seen that the GUWM22 can be used for preventing and treating anthracnose of the picked peppers (line peppers). The Trichoderma aureum GUWM22 is a new biocontrol Trichoderma strain with good potential. The invention provides a new strain resource and an environment-friendly and efficient biocontrol approach for effective prevention and treatment of pepper anthracnose.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention provides application of a CaCRY2 gene in regulating and controlling resistance of plants to phytophthora capsici, and belongs to the technical field of plant molecular biology and plant genetic engineering. The gDNA sequence of the CaCRY2 gene is as shown in SEQ ID NO: 1, and the cDNA sequence of the CaCRY2 gene is as shown in SEQ ID NO: 2; the expression of the CaCRY2 gene is inhibited, and the resistance of the plant to phytophthora capsici is enhanced; the overexpression of the CaCRY2 gene weakens the resistance of the plant to phytophthora capsici, and the plant is a solanaceae plant. The invention provides a capsicum phytophthora blight related gene CaCRY2 and application thereof. The resistance of capsicum plants to phytophthora blight can be enhanced by silencing the CaCRY2 gene through a virus-induced gene silencing technology; the resistance of the tobacco plants to epidemic diseases can be weakened by overexpressing the CaCRY2 gene in the tobacco plants through a transgenic technology; the invention provides a favorable basis for improving the disease resistance of plants and cultivating disease-resistant varieties.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses a litchi disease-resistant gene LcEXP1 and an application thereof. The litchi disease-resistant gene LcEXP1 is separated from litchis, the full-length cDNA (complementary deoxyribonucleic acid) of the gene is 750bp, and the encoded protein is an expansion protein with the full length consisting of 250 amino acids. The litchi disease resistance related gene LcEXP1 is transiently expressed in tobacco, different tobacco leaves are inoculated with phytophthora capsici and alternaria alternata after two days, it can be observed that after expression of the LcEXP1, scabs are remarkably reduced compared with negative control, and biostatistical analysis shows that the areas of the two treated scabs are remarkably different. Therefore, the gene can effectively promote the disease resistance of plants such as tobacco and the like, and has a wide application prospect in developing products for improving the disease resistance of the plants.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention provides a streptomyces hygroscopicus engineering strain as well as construction and application thereof, and relates to the technical field of biology, the engineering strain takes streptomyces hygroscopicus CL-1 as a starting host and is obtained by heterologous expression of an epsilon-polylysine synthetase encoding gene pls, and the nucleotide sequence of the epsilon-polylysine synthetase encoding gene pls is shown as SEQ ID NO.1. Streptomyces hygroscopicus CL-1 is used as a host, the epsilon-polylysine synthetase coding gene derived from streptomyces albus is subjected to heterologous expression, the obtained recombinant strain has high epsilon-polylysine production capacity, and under the optimized fermentation condition, the yield of epsilon-polylysine is 0.87 g / L, and industrial scale production requirements are met. Meanwhile, the broad-spectrum antibacterial activity to various plant pathogenic fungi and common bacteria, including colletotrichum gloeosporioides, fusarium oxysporum, phytophthora capsici and the like, is also remarkably improved. Therefore, the streptomyces hygroscopicus has important application in the aspects of production of epsilon-polylysine, prevention and control of pathogenic microorganisms, preservation and the like, and the industrial and agricultural utilization potential of the streptomyces hygroscopicus is expanded.

The invention belongs to the technical field of microorganisms, and discloses a Trichoderma brevicompactum 6311 with phytophthora capsici biological control ability, the name of the Trichoderma brevicompactum 6311 is 6311, the classification name of the Trichoderma brevicompactum is Trichoderma brevicompactum 6311, the preservation number of the Trichoderma brevicompactum 6311 is CGMCC No.41489, the preservation date of the Trichoderma brevicompactum 6311 is August 13, 2024, and the preservation unit of the Trichoderma brevicompactum 6311 is China General Microbiological Culture Collection Center, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The fermentation liquor of the trichoderma 6311 has the capability of inhibiting the growth of phytophthora hyphae on a flat plate, and the inhibition rate reaches 100%. The germination rate, radicle and hypocotyl length, growth index and fresh weight of the pepper seeds soaked in the 6311 fermentation liquor are improved. The research shows that the trichoderma brevicompactum 6311 has biological control potential and provides a theoretical basis for application of the trichoderma brevicompactum 6311 as a biological bactericide.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses a seed priming method for improving the early disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds in sodium hypochlorite and then washing with sterile deionized water; S2, pulse phosphorylation activation treatment: adding seeds and activation solution to a pressure-resistant polypropylene container connected to a vacuum pipeline, evacuating to 0.08 MPa after sealing, returning to normal pressure after maintaining for 5-10 minutes, and then transferring to a constant temperature oscillator for phosphorylation activation treatment; S3, immune training treatment: transferring the seeds after phosphorylation activation treatment to a priming solution containing a suspension of UV-inactivated Phytophthora capsici spores and H2O2, and intermittent ventilation treatment; S4, stabilization treatment: transferring the seeds after immune training treatment to an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain pepper seeds. The present invention solves the problem of immune response interruption caused by insufficient signal intensity and rapid protein turnover when stimulating using a simulated environment in the prior art.

The invention discloses a dsRNA nano preparation for preventing and controlling phytophthora capsici as well as a preparation method and application of the dsRNA nano preparation. The nucleotide sequence of the phytophthora capsici sugar transporter protein HXT4 gene is as shown in SEQ ID NO.1, and the ASPN-dsRNA nano preparation is obtained by mixing dsRNA as shown in SEQ ID NO.2 and amino-modified mesoporous silica. By exogenous application of the ASPN-dsRNA nano preparation, gene expression silencing of the target gene phytophthora capsici sugar transporter HXT4 is induced, so that pathogenicity of phytophthora capsici is reduced or even lost, and the purpose of protecting crops is achieved. The preparation can effectively reduce the pathogenicity of phytophthora capsici, the inhibition rate on phytophthora capsici infection can reach 72%, and the prevention and control efficiency on phytophthora capsici reaches 87%. The ASPN-dsRNA nano preparation is high in stability and obvious in prevention and control effect, and provides a new prevention and control strategy and means for developing a precise prevention and control method for crop epidemic diseases.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The invention discloses a dehydroabietyl quaternary ammonium salt of which the chemical structural formula is shown in the following formula. The dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani, and the dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani. The method has potential value in preparation of green pesticide bactericides, and can reduce adverse effects of the bactericides on the environment and human beings; # imgabs0 #