346 results about "Phytophthora capsici" patented technology

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU / g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses a trichoderma asperellum strain and application thereof. The trichoderma asperellum strain is named as trichoderma asperellum Thz01, and the preservation number of the trichoderma asperellum strain is CGMCC (China general microbiological culture collection center) No.6422. The trichoderma asperellum strain is separated from a capsicum habitat, has a remarkable antagonism effect to phytophthora capsici, can be obviously subjected to hyperparasitism on the phytophthora capsici, an can effectively degrade mycelia of the phytophthora capsici, and a biocontrol agent prepared from the strain can be used for effectively preventing and treating phytophthora capsici leonian .

The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g / L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.

Cyazofamid belongs to sulfanilamide imidazole bactericide and has high bioactivity on oomycete fungi such as phytophthora capsici, downy mildew, pseudo downy mildew and pythium and brassica plasmodiophora brassicae of plasmodiophoromycetes. A preparation method for cyazofamid aqueous emulsion has a formulation comprising 1 to 30 percent of the cyazofamid, 2 to 15 percent of emulsifying agent, 0.2 to 5 percent of co-emulsifying agent, 10 to 20 percent of solvent, 10 to 20 percent of latent solvent, 0.1 to 5 percent of anti-freezing agent, 2 to 10 percent of stabilizing agent, 0.1 to 2 percent of defoaming agent, 0.1 to 5 percent of thickening agent, 0.1 to 4 percent of pH value conditioner and the balance of deionized water, and comprises the following steps: adding the latent solvent into the deionized water according to a ratio; dispersing the latent solvent in a high-shear preparation kettle and stirring the solution evenly; adding cyazofamid raw powder and solvent as well as latent solvent mother solution into the solution; shearing the mixed solution at high speed; and finally adding the thickening agent into the mixed and stirring the resulting solution evenly to obtain the cyazofamid aqueous emulsion. The cyazofamid aqueous emulsion is a new environment-friendly water-based dosage form of agricultural bactericide, uses water as a dispersive medium to replace an expensive organic solvent, reduces environment and dust pollution, and is a new dosage form with development prospect.

The invention discloses a Rhodopseudomonas strain. The name of the Rhodopseudomonas strain is Rhodopseudomonas PSB-06, and the collection number is CCTCC NO:M2012518. The invention also discloses a biocontrol microbial inoculum which is obtained through the activation, seed culture and production culture of the strain. The preparation of the biocontrol microbial inoculum comprises the multiple steps of activation, seed culture and production culture. The invention also discloses a Rhodopseudomonas strain biocontrol fermentation liquid which is prepared from the biocontrol microbial inoculum through fermentation and centrifugation. Each of the Rhodopseudomonas strain, the biocontrol microbial inoculum and the biocontrol fermentation liquid can be used in antagonizing phytophthora capsici or controlling phytophthora blight of pepper.

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

The present disclosure provides pepper plants exhibiting resistance to Phytophthora capsici. Such plants may comprise novel introgressed chromosomal regions associated with disease resistance. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The invention belongs to the technical field of biology and in particular provides a pectate lyase (PL) gene Pcpel1 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on theinoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PL gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The invention relates to a single spore isolation method of phytophthora capsici zoospores. The single spore isolation method comprises the steps that phytophthora capsici is inoculated to a V8 culture medium, sealing is performed with a sealing membrane, culture at the constant temperature of 25 DEG C is performed for 5 days, then the sealing membrane is removed, irradiation is performed for 24 hours, sterile water of 4 DEG C is added to perform induction and constant-temperature release respectively for 25 minutes so as to obtain a zoospore suspension, the suspension is diluted to reach the standard that 1-2 spores exist in each (10*) view, 1 mL of the suspension is taken and added to a V8 culture medium flat plate containing antibiotics in an evenly coated mode, constant-temperature standing is performed for 6 hours, a single germinated zoospore is found through an inverted microscope and is cut by using a scalpel to form a 0.5 cm square mark, it is confirmed that single spores are transferred to a flat antibiotic plate, and purified spores are single spore plants. The single spore isolation method is simple and convenient to operate, high in accuracy and good in rapid separating effect, adopts simple devices and can separate single spores of a large number of phytophthora capsici zoospores within a short time.

The invention discloses bacillus velezensis ZF481 and application thereof. The strain number of the bacillus velezensis is ZF481, the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center on July 8, 2020, and the registration number of the bacillus velezensis is CGMCC No.20320. The bacillus velezensis ZF481 or / and the metabolite of the bacillus velezensis ZF481 can be used for preventing and / or treating the plant clubroot, or preparing a product for preventing and / or treating the plant clubroot. The strain has an inhibiting effect on the activity and germination of plasmodiophora brassicae resting spores, has a good growth promoting effect on brassicaceous crops such as Chinese cabbage and the like, and also has a good antibacterial effect on phytophthora capsici, stemphylium solani, fusarium oxysporum, alternaria solani, corynespora, Botryis cinerea, rhizoctonia solani and the like.

The utility model relates to a sterilization composite containing azoxystrobin and application. The composite mainly comprises two compounds A and B with effective quantity, the A is the azoxystrobin, the B is selected one from myclobutanil, flusilazole, mancozeb and dimethomorph, and the compounds A and B have the following parts by weight: 50-1 and 1-50. The composite can be used for preventingand curing powdery mildew, rust disease, downy mildew, anthracnose, phytophthora infestans, leaf spot, cladosporium cucumerinum and the like on frumentum, vegetables and fruit trees, and particularlyhas good effect on sigatoka, erysiphe graminis, phytophthora capsici leonian, cucumber downy mildew and venturia nashicola.

The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, belonging to the field of molecular biology. A pair of specific primers aredesigned and synthesized by the cayenne (sweet) pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitative and quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carriedout. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency management for the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.

The invention relates to an microbiological viable bacteria preparation for prevention and cure of cayenne pepper fungus soil transferred diseases and process for preparation and use, wherein the active constituent of the preparation is actinomycete, which can produce antibiotics and can be fixed into the rhizosphere soil and root of the cayenne pepper, its mass propagation can repress the growth of Phytophthora capsici. The preparation is produced by solid state culturing method from farm and side line products as raw material. The major objective for prevention and cure of the invention is Phytophthora capsici.

The invention discloses a paenibacillus polymyxa strain and application thereof. The strain belongs to paenibacillus polymyxa strains YT9, and the preservation number of the strain is CGMCC NO.15958.The separated strain not only has a strong inhibition effect on phytophthora sojae but also has a great antagonism effect on plant pathogenic oomycetes and phytopathogenic fungi, wherein the plant pathogenic oomycetes include phytophthora capsici, phytophthora nicotianae and the like, and phytopathogenic fungi include wheat sheath blight bacteria, wheat root rot bacteria, cercospora personata, cercospora arachidicola and the like; the bacteriostatic effect on phytophthora sojae is the strongest, wherein the bacteriostatic rate reaches 86.27%+ / -1.62%. In addition, the paenibacillus polymyxa strain YT9 is separated from healthy soybean rhizosphere soil and is free of ecological toxicity, high in safety, wide in bacteriostatic spectrum, stable and lasting in bacteriostatic activity and easy to culture. The strain has a broad application prospect in biological control over plant diseases.

The invention discloses streptomyces antibioticu and biological preparation prepared from an active product thereof and application. The streptomyces antibioticu is a strain which is screened from more than 2,000 marine actinomycetes and has good inhibiting activity on phytophthora capsici, phytophthora cucumisativus, colletorichum cucumisativus and colletotrichum capsici; and the name of the strain is streptomyces antibioticu H74-18, the strain was collected in the China Center for Type Culture Collection on December 27th, 2010, and the collection number is CCTCC NO: M2010364. Antimycin A18(1) with bacteriostatic activity is separated from the strain, the molecular formula of the antimycin A18(1) is C28H41N2O9, and the molecular weight of the antimycin A18(1) is 549.3. The antimycin A18(1), an emulsifier and methanol are mixed to form the biological preparation with good stability. In 12 days after the biological preparation is sprayed, under the application times of 500x, the effect of preventing and controlling the phytophthora capsici is 90.00 percent, the effect of preventing and controlling the phytophthora cucumisativus is 86.67 percent, the effect of preventing and controlling the colletotrichum capsici is 86.67 percent and the effect of preventing and controlling the colletorichum cucumisativus is 90.00 percent.

The invention relates to a fluorescence quantitative polymerase chain reaction (PCR) detection method and a detection kit for phytophthora capsici leonian. In the method, a primer pair ljym1 / ljym2 is designed according to an ITS area of the phytophthora capsici leonian rDNA; the reaction liquid contains the pair of specific primers and fluorescent dye; a phytophthora capsici leonian DNA template is added into the reaction liquid; and the fluorescence intensity of the reaction system is detected in real time along with the PCR reaction, and a Ct value is calculated according to analysis software so as to calculate the content of phytophthora capsici leonian in a sample. The kit designed according to the method has simple and fast operation and good specificity and high sensitivity, is free from the culture conditions based on the nucleotide detection, can actually reflect the infection conditions of phytophthora capsici leonian into plants by quantitative detection, realizes high-flux sample detection at the same time, conducts fast quantitative detection on the phytophthora capsici leonian and replaces the conventional identification method of isolated culture on a selective medium, and is suitable for wide popularization and application in the field of plant disease diagnosis and detection.

The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environmentin diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.

The invention relates to novel high-efficiency biocontrol bacterial fertilizer and a production technology thereof. The novel high-efficiency biocontrol bacterial fertilizer is named as Lvdu I. The novel high-efficiency biocontrol bacterial fertilizer is an active microbe-containing specific preparation prepared through activity recovery, propagation and expansion culture of strains, preparation of liquid bacterial fertilizer, preparation of solid bacterial fertilizer, and the like. The novel high-efficiency biocontrol bacterial fertilizer can treat multiple soil-borne diseases such as cucumber fusarium wilt, phytophthora capsici leonian, tomato bacterial wilt and the like. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I can improve soil fertility, prolong fertilizer action time, promote growth of a few of crops, increase output, produce biocontrol effects on harmful microbes, and reduce crop diseases. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I has the advantages of low costs, convenience of use, simple process flow, and no pollution and toxic residues.

The invention relates to a specific application of a nascent polypeptide complex protein, in particular to an application of a nascent polypeptide-associated complex (NAC) protein as a vegetable immune protein. Specifically, the application can be an application of the NAC protein in improving the gray mold resisting immunity of tomatoes, an application of the NAC protein in improving the tobacco mosaic virus resisting immunity of tomatoes, or an application of the NAC protein in improving the phytophthora capsici resisting immunity of chilli. The application process has simplicity of operation, low cost and good effect.

The invention relates to a preparation method of an anti-phytopathogen fermentation liquid of a spirea endophytic fungus. The spirea endophytic fungus is named Alternaria alternata XXB07 and was collected in China General Microbiological Culture Collection Center on March 8, 2013; and the collection number is CGMCC No.7292. The method comprises the following operation steps: 1. performing strain activation; 2. performing seed culture, and 3. performing fermentation culture to obtain a fermentation liquid of the spirea endophytic fungus, filtering the fermentation liquid under aseptic conditions to remove mycelia, and passing through a 0.22-micrometer filter to obtain the antimicrobial fermentation liquid of the spirea endophytic fungus. The invention confirms the preparation method of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 strain having antibacterial activity; and antimicrobial tests of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 discover that a fermentation liquid eluate and a mycelium extract of the strain have mycelium growth inhibition action on plant pathogenic fungi of Phytophthora capsici.

The invention discloses an effector protein derived from phytophthora capsici as well as a coding gene and application thereof. The protein disclosed by the invention is (a) or (b) or (c): (a) protein consisting of amino acid sequences represented in the 19th-476th sites of a sequence 1 of a sequence list; (b) protein consisting of an amino acid sequence represented in the sequence 1 of the sequence list; and (c) protein obtained by substituting and / or deleting and / or adding the amino acid sequence of the protein defined by (a) or (b) through one or more amino acid residues and having an effector function. Experiments prove that the protein disclosed by the invention effectively participates in a process that phytophthora capsici infects a pepper host and has a function of inducing plant tissue cell death and even an obvious necrotic symptom on an infected part. The effector protein disclosed by the invention is significant for enriching molecular mechanism information of plant pathogenic oomycetes and host interaction and for establishing an oomycetes disease integrated control technological strategy.

The invention belongs to the technical field of biology and in particular provides a polygalacturonase (PG) gene Pcipg5 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on the inoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PG gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The invention discloses a new Pseudomonas corrugate strain (P94 GMCC No.1895), which is characterized by the following: manufacturing relative HCN to prevent bacteria, proteinase and phospholipase and IAA; inhibiting partial plant pathogenic fungus (Botrytis cinerea, Ceratocystis fimbriata, Monilinia laxa, Magnaporthe grisea, Pythium aphanidermatum and Phytophthora capsici) and partial pathogenic bacteria (Pseudomonas syringae, Acidovorax avenae and Ralstonia solanacearum); accelerating the growth of tomato and cucumber to improve production by 22%-28%.