68 results about "Seed priming" patented technology

The present invention provides means and methods for simple and efficient introduction of foreign genetic material into the plant cell. Particularly, the present invention combines seed priming and virus-based DNA constructs for efficient introduction of heterologous DNA into plants.

The invention belongs to the technical field of seed priming, and discloses a mixed type agent for restoring activity of a deteriorated rice seed embryo, a preparation method and an application of the mixed type agent. The mixed type agent comprises the raw materials such as a vine tea extract, a tea extract, an N element fertilizer, a P element fertilizer, a K element fertilizer and a humic acid fertilizer; and the parts by weight of the vine tea extract, the tea extract, an effective component N in the N element fertilizer, an effective component P2O5 in the P element fertilizer, an effective component K2O in the K element fertilizer and an effective component humic acid in the humic acid fertilizer are respectively 0.50-41.50 parts, 24.50-25.50 parts, 6.75-7.35 parts, 10.25-10.75 parts, 13.50-14.50 parts and 2.25-2.75 parts. The mixed type agent is reasonable in formula, fully utilizes respective characteristics of all raw materials, is reasonable in blending, and meets the requirements of all functions and nutrients required by restoring the activity of the rice seed embryo.

The invention belongs to the technical field of seed vigor improvement and seed germination promotion, and discloses a method for promoting germination of oat aged seeds and growth of seedlings. The method comprises the following steps: firstly, determining the initial water content of the oat aged seeds to be tested, and after adjusting the water content, soaking the oat aged seeds for 24-36 hours by using a melatonin solution with the concentration of 1 (mu) M-1,000 (mu) M, then taking out the oat aged seeds to be tested and washing with distilled water; and finally, drying until the water content of the oat aged seeds to be tested is equal to the initial water content. In an existing seed priming technology, priming time and concentrate of a priming agent are affected by kinds of seeds and initial quality of the seeds and are not determined, specific parameters of the oat aged seeds primed by melatonin are determined by research, improvement and application of a technology of priming and treating the oat aged seeds by using the melatonin are realized, and the standard operation method for promoting germination of the oat aged seeds and improving vigor of the aged seeds is provided.

Random numbers are used for a variety of purposes and play key roles in systems such as simulation studies, information processing, communication, and encryption. Truly random numbers are generally the result of processes that cannot be successfully repeated to generate the same sequence of results, and thus are unpredictable. Pseudo-random numbers, on the other hand, are easily replicated as the output of deterministic processes. Such a series of pseudo-random numbers will eventually repeat itself in perpetuity once it has exhausted its cycle length. The claimed invention introduces a new class of random numbers called idem-random numbers that have many of the essential characteristics of random numbers but which may be successfully replicated at different locations and at different times and which will never repeat themselves. The claimed invention uses the characteristics of prime and prime-like numbers to create endless sequences of idem-random numbers. Idem-random numbers are produced by a.) identifying a seed prime or prime-like number, b.) identifying a process or condition for determining a subsequent prime or prime-like number, c.) applying that process or condition in an iterative fashion to yield a sequence of prime or prime-like numbers, d.) identifying a mathematical relationship or property which can be applied to two or more numbers in that prime or prime-like number sequence, and e.) applying that mathematical relationship or property to the sequence of numbers to provide a set of idem-random numbers. Optional application of a distribution-transformation process may beneficially create final idem-random numbers with specified distribution characteristics.

The invention discloses an apple breeding method. The method comprises the following steps: respectively conveying pollen of red-flesh and crisp-flesh strains to a castrated apple variety having excellent comprehensive characters to obtain backcross seeds; taking helium gas as a working medium, and performing non-ionizing radiation treatment on the seeds in a vacuum closed environment; sequentially performing seed soaking and seed priming treatment; building a seedling raising bed composed of a plurality of 8*10cm seedling raising troughs in a greenhouse, and filling self-made planting soil in each seedling raising trough; after the filling operation is completed, gently pressing the primed seeds into the planting soil according to a standard that the seeds are just covered by mud, wherein two seeds are sown in each seedling raising trough; and the seed sowing operation is completed, covering a semitransparent film on the seedling raising bed, and perforating the covered semitransparent film corresponding to the places where the seeds are sown and around the places where the seeds are sown. According to the invention, the breeding period is shortened, and the germination rate and transplanting survival rate are greatly improved, thereby enabling the population yield to reach the maximum and increasing the fine fruit rate.

Acetolactate synthase inhibitors, such as imazapyr and pyrithiobac and mixtures thereof, prepared as slow-release formulations are useful for the preparation of seed dressing, seed priming, seed or particle-substrate coating herbicidal compositions for control of parasitic weeds such as Orobanche spp., Striga spp. and Alectra spp. The use of agrochemicals can be rendered more efficient when coated or bound as a slow release formulation. Particles used as the substrateto be coated may be plant seeds or particles made of a strong or weak ionic resin or a biodegradable carbohydrate natural polymer, a modified polymer, or artificially lignified cellulose. The herbicidal formulation may be covalently linked or adsorbed to the surface of the particle. The same slow release formulations are invaluable for preventing rapid herbicide leaching in agricultural as well as non-agricultural weed control situations.

The invention belongs to the field of research on a rice seed priming technology, and discloses application of an ampelopsis grossedentata extract serving as a trigger enhancer of a rice deteriorating seed. The ampelopsis grossedentata extract is the ampelopsis grossedentata extract product which is prepared from ampelopsis grossedentata serving as raw materials extracted by a solvent extraction method, a supersonic extraction method or a chromatographic separation method; the ampelopsis grossedentata extract comprises a solid product, a liquid product and a paste product, preferably the solid product. The ampelopsis grossedentata extract is a natural plant extract, so that the ampelopsis grossedentata extract is free of toxicity, low in price, safe when being used for triggering the rice deteriorating seed, and free of environmental pollution. The ampelopsis grossedentata extract has a significant effect on improvement of seed triggering as the enhancer.

The invention discloses an industrialized seedling production method of panax notoginseng. The industrialized seedling production method comprises following steps: selecting fresh seeds; utilizing plant hormones to pre-germinate and using perlites to perform seed priming operation; and utilizing floating seedlings to perform seedling management; and transplanting seedlings after panax notoginseng seedlings grow up to 3-5 centimeters. The industrialized seedling production method of panax notoginseng has following beneficial effects: the method is easily operated and can efficiently culture quality panax notoginseng seedlings under the industrialized control condition and can effectively prevent soil-borne diseases during cultivation of panax notoginseng seedlings so that diseases during cultivation of panax notoginseng are decreased.

The invention discloses a seed priming solution and method. The seed priming solution comprises a priming agent and an oxygenate, wherein the oxygenate is hydrogen peroxide. According to the seed priming solution, hydrogen peroxide is used as the oxygenate and is added into the seed priming solution for seed priming, and no additional priming and oxygenating devices and stirring work are needed, so that the priming operation procedure is greatly simplified. At the same time, hydrogen peroxide can be cooperated with the priming agent such as gibberellin or salicylic acid which is frequently used in the existing priming technology to promote seed germination, so that the seed priming effect is remarkably improved.

The invention provides a tobacco seed primer, a preparation method of the tobacco seed primer, and a tobacco seed priming method, and relates to the technical field of plant cultivation. The tobacco seed primer provided herein is made from calcium chloride, gibberellin, betaine, proline, salicylic acid, sodium nitroprusside, and trehalose. The tobacco seed primer is capable of enhancing the resistance of tobacco seeds to low temperature stress, drought stress and low temperature and drought cross-stress, and is effective for increasing emergence rate of tobacco seeds and the quality of seedlings. In addition, the invention provides the preparation method of the tobacco seed primer; by dissolving calcium chloride, gibberellin, proline, salicylic acid, trehalose, betaine, and sodium nitroprusside sequentially in water, it is possible to effectively ensure the priming effect of the primer. In addition, according to the priming method of tobacco seeds provided herein, by using the tobacco seed primer provided herein, it is possible to enhance the cold resistance and drought resistance of tobacco seeds.

The invention belongs to the technical field of rice seed priming research, and discloses an application of a vine tea extract in improving the germination rate of deteriorated seeds of hybrid rice. The vine tea extract is a vine tea extract product extracted and obtained by methods such as solvent extraction, ultrasonic extraction or chromatographic separation based on vine tea as a raw material. The product comprises a solid product, a liquid product and a paste product, preferably the solid product. The vine tea extract is a natural plant extract and is free from any toxicity and low in cost, and is free from environmental pollution, and used for initiating safety of seeds of hybrid rice. The vine tea extract has a remarkable effect on initiation of seeds of hybrid rice.

The invention discloses a herbaceous flower seed priming method. The method comprises the following steps: 1) carefully selecting seeds; 2) preparing a polyethylene glycol solution with an osmotic potential being between -0.5 and -2.0 Mpa; 3) pouring a PEG solution into a container, putting a semi-permeable membrane with a same size on the solution, putting the seeds to be treated on the semi-permeable membrane, allowing the container to stand at 10 DEG C-20 DEG C for 7-14 days; 4) drying at 25 DEG C for 24 hours, or directly sowing without drying. The invention adopts a semi-permeable membrane to control the water absorption process of the seeds, avoids direct contact of the seeds with the solution, is applicable to the priming treatment of high-value herbaceous flower seeds, and is especially applicable to seeds with mucus on surfaces such as pansy, and particle seeds such as petunia hybrida.

The invention discloses a hydro-priming method for Chinese pine seeds. In the method, the Chinese pine seeds are put in a plastic plate for priming seeds, and under dark conditions, the temperature of a working room is controlled to 10 DEG C; during the seed priming period, after 0h, 8h, 16h, 40h, 64h and 88h, based on the total water absorption amount, 53.6 percent, 14.5 percent, 14.5 percent, 8.3 percent, 5.7 percent and 3.4 percent of water are added into the plastic plate respectively, wherein the total addition amount of water is 30 percent based on the original mass of the seeds, and the priming period is 10 days; during the priming period, the plastic plate is periodically and fully stirred to guarantee the uniform water absorption of the seeds; and the treated seeds can be directly used for sowing, or re-dried for storage. The method has the advantages of obviously improving activity and resistance of the Chinese pine seeds and the seedlings burgeoned by the Chinese pine seeds, solving the problem that the seed quality is low, the problem that the burgeoned seedlings are weak in activity and resistance, the problem that the seeds cannot be stored again, and the problem that the seedling survival rate of the seeds is low when the seeds are used for direct seeding forestation or air seeding forestation in the Chinese pine seeds treated by the conventional seed-soaking sprouting, along with simple method, easy mastering of the technique, low seedling-growing cost and easy popularization.

The invention discloses a method for increasing the germination rate of gourd seeds, belonging to the technical field of vegetable seed production. According to the method for increasing the germination rate of the gourd seeds, a seed priming solution prepared from an acide phosphorique is used for soaking the gourd seeds for priming; the method concretely comprises the steps of soaking the seeds in the seed priming solution prepared from the acide phosphorique, and drying for realizing seed priming. After the method provided by the invention is used for priming the gourd seeds having lower germination rate, the germination rate of the gourd seeds can be increased to 85% or above, the germination uniformity of the gourd seeds is obviously improved, the gourd seeds still have qualified germination rate after being placed for three months. The method provided by the invention is simple in operation, high in controllability and low in cost, is capable of effectively priming the gourd seeds, is obvious in priming effect, has better production application value, and is suitable for large-scale production.

The invention discloses a selenium-rich Paeonia Suffruticosa planting method. The selenium-rich Paeonia Suffruticosa planting method includes the steps of (1), selecting dry soil exposed to the sun and good in drainage effect and applying a selenium-containing base fertilizer, phoxim granules and carbendazim; (2), performing seed priming; (3), accelerating germination to enable bud seedlings to be as tall as 9-12cm; (4), transplanting the Paeonia Suffruticosa bud seedlings into the soil applied with the selenium-containing base fertilizer as deep as 35-45cm and performing topdressing; (5), in 4-5 days before a flowering stage, spraying flower heads of plants with sodium selenite aqueous solution, covering the flower heads with transparent plastic bags, pricking the plastic bags, removing the plastic bags on the flower heads of the plants in the flowering stage and performing artificial pollination; (6), from the 6th-8th day to the 4th day before Paeonia Suffruticosa seed picking, applying 2-3cm of stalk and a leaf closest to every Paeonia Suffruticosa fruit with the sodium selenite aqueous solution every other day. The organic selenium content of Paeonia Suffruticosa seeds obtained through planting by the selenium-rich Paeonia Suffruticosa planting method is 0.28-0.30mg / kg.

The invention belongs to the technical field of seed germination and storage and particularly relates to a method for enhancing high-temperature-resistant storage of oat primed seeds through melatonin. The method includes the following steps that oat seeds for testing are selected; 2, the water content of the seeds is adjusted; 3, seed priming is carried out; 4, high-temperature storage simulation is carried out; 5, the high-temperature-resistant storage conditions for melatonin to prime seeds are determined. The method can serve as an effective method for enhancing the high-temperature-resistant storage performance of the oat primed seeds and promoting seed germination and seedling growth.

The invention discloses a formula of a seed coating agent for controlling a wheat take-all disease. The seed coating agent is composed of a sterilizing agent and a seed priming and resistance inducer,the sterilizing agent is composed of silthiopham and probenazole, the seed priming and resistance inducer is composed of salicylic acid, brassinolide, and gibberellin; the seed coating agent is prepared from the compositions in percentage by mass: 2%-40% of the sterilizing agent, 0.0005%-0.05% of the salicylic acid, 0.00001%-0.001% of the brassinolide, and 0.001%-0.1% of the gibberellin. A formedmixture has the good action of promoting seed germination and seedling strengthening on wheat seeds. According to the formula of the seed coating agent for controlling the wheat take-all disease, thesilthiopham and the probenazole both have low toxicity, and the safety is achieved on people and livestock, beneficial organisms and the environment; and drug resistance of germs to single agents canbe delayed.

The invention provides a rice seed priming agent for low-oxygen adverse conditions and a use method thereof, and belongs to the technical field of rice planting. The rice seed priming agent for the low-oxygen adverse conditions comprises a seed priming agent and a seed stress buffering agent, each litre of the seed priming agent is prepared from 80-120 g of PEG, 0.1-0.6 mg of salicylic acid and 0.1-0.5 mg of jasmonic acid, and each litre of the seed stress buffering agent is prepared from 20-30 g of glucose, 20-30 g of saccharose, 10-20 g of alpha-ketoglutaric acid, 10-20 g of fumaric acid, 10-20 g of L-glutamine, 10-20 g of ascorbic acid and 25-35 g of citric acid. According to the rice seed priming agent for the low-oxygen adverse conditions and the use method thereof, on the basis of utilizing seed priming for improving the stress resistance, according to limiting factors possibly existing in the seed germination process under the anaerobic stress condition, different priming agents and seed soaking agents are used, therefore, the seed germination capacity is improved, and the seed germination rate is increased.

The invention discloses a method for corn seed priming. The method comprises the following steps: selecting corn seeds and treating the seeds with disinfection, cleaning and drying; dissolving polyamine in water to obtain a polyamine aqueous solution, and making a treating fluid after mixing; adding the treating fluid to the corn seeds and conducting low-temperature priming; and cleaning and re-drying the corn seeds which are already primed, and conducting sowing or low-temperature storage. The method is easy to operate and master and short in duration, and can promote germination and growth of seedlings and enhance cold resistance in case of low temperatures after corn sowing.

The invention relates to a method for processing seeds before seeding, and in particular relates to a method for breaking the hardness of licorice seeds by using foamed acid. The invention aims at providing a method for breaking the hardness of licorice seeds by using foamed acid which can be used for quickly, safely and efficiently breaking hardness of licorice seeds, reducing burn area, preventing an 'imbibition' phenomenon in the seed processing process, and keeping the vitality of seeds, and can be operated on a large scale. The method for breaking the hardness of licorice seeds by using foamed acid provided by the invention mainly comprises the following steps: preparing the foamed acid; mixing the foamed acid and the seeds; and cleaning and drying in shade of the seeds. According to the method for breaking the hardness of licorice seeds provided by the invention, the operation is safe, and sulfuric acid and labor are saved; the licorice seeds produced by the method provided by the invention are small in burn area, high in vitality index and germination index, high in rate of emergence, and beneficial to seed priming, coating and pelleting. The method provided by the invention is used in the fields of production and processing of the licorice seeds, and licorice artificial cultivation.

A rapid wetland planting method for ecological restoration comprises the steps of seed filling, power source starting, downward handle pressing, planting completing and the like, a planter is includedin the planting method, the planter comprises a columnar outer shell, an inner shell is connected to the upper wall of the inner side of the outer shell in a sliding mode, and an outer shell of the outer shell is connected to the lower wall of the inner side of the inner shell in a sliding mode. The lower side of the outer wall of the outer shell is sleeved with a net plate and a sliding ring ina sliding mode, the sliding ring is connected with the inner shell in a sealed and sliding mode, the sliding ring and the outer edge of the net plate are jointly sealed and glued with an elastic film,a push rod is fixed to the upper wall of the net plate, and the outer wall of the outer shell is in threaded connection with a trigger ring. The planting device has the advantages that planting workcan be started after seeds are placed in the storage bin, the planting device is moved to the to-be-planted position of the wetland, the handle of the planter is pressed downwards, and the power assembly and the sowing assembly act together to rapidly plant the seeds at the corresponding depth.

The present invention relates to a seed treatment method, particularly to an solanaceous fruit seed priming method, which comprises: adopting vermiculite (a ratio of seed to vermiculite to water is 1:3:3-4) to prime for 4-8 days at a temperature of 15-20 DEG C under a dark condition, wherein trichoderma is added during seed priming, and a use concentration is 1*10<6>-4*10<7> viable spores / L; and re-drying for 1-2 days at a temperature of 25 DEG C. The priming method can be used for seed treatments of tomatoes, hot peppers, and eggplants.

The invention discloses a method for improving vitality of tobacco seeds on the basis of seed priming. The method comprises the following steps: 1) soaking seeds: putting tobacco naked seeds into water according to the proportion of 1kg:(2-6)L, and ventilating and soaking under the condition of normal temperature for 2.5 to 3.5 hours; 2) disinfecting: putting the soaked seeds into a copper sulfatesolution with the concentration of 4 to 8 g / L, soaking for 10 to 30 minutes, taking on the seeds which float on the surface of the solution, and after disinfecting, completely cleaning the remained seeds with clean water; 3) initiating: putting the disinfected seeds into an initiator, initiating under the condition of full illumination at the initiating temperature of 15 to 25 DEG C for 12 to 48hours, taking out the seeds and completely cleaning; and 4) drying. By the method, the tobacco seeds are subjected to normalized seed soaking, disinfecting, initiating, drying and storage treatment, so that the vitality of the tobacco seeds is effectively improved, the tobacco seeds can be germinated rapidly under the stress conditions of low temperature and the like, the germination rate is increased and seed dormancy can be relieved.

The invention provides a rice seed priming agent for low-oxygen adverse conditions and a use method thereof, and belongs to the technical field of rice planting. The rice seed priming agent for the low-oxygen adverse conditions comprises a seed priming agent and a seed stress buffering agent, each litre of the seed priming agent is prepared from 80-120 g of PEG, 0.1-0.6 mg of salicylic acid and 0.1-0.5 mg of jasmonic acid, and each litre of the seed stress buffering agent is prepared from 20-30 g of glucose, 20-30 g of saccharose, 10-20 g of alpha-ketoglutaric acid, 10-20 g of fumaric acid, 10-20 g of L-glutamine, 10-20 g of ascorbic acid and 25-35 g of citric acid. According to the rice seed priming agent for the low-oxygen adverse conditions and the use method thereof, on the basis of utilizing seed priming for improving the stress resistance, according to limiting factors possibly existing in the seed germination process under the anaerobic stress condition, different priming agents and seed soaking agents are used, therefore, the seed germination capacity is improved, and the seed germination rate is increased.

The present invention relates to a method of eliciting plant immune responses based on seed priming by using the heat (high temperature)-treated culture solution of Bacillus spp, and the high pressure-sterilized culture solution of Bacillus sp. strains PB69 and 1628 which have been selected in the present invention exhibits an activity of eliciting induced systemic resistance similar to BTH, and also has an effect for inducing promoted plant growth and controlling plant diseases, and thus it is expected to contribute to enhance the crop productivity.

The invention relates to a medicago sativa seed priming solution which comprises the following components in percentage by mass: 140-180mM of KNO3, 120-160mM of KH2PO4 and 0.1-0.2mM of salicylic acid. The preparation and priming method of medicago sativa seed priming solution comprises the following steps: (1) preparing priming solution raw materials; (2) preparing a priming solution; (3) treating the seed priming solution; (4) washing the seeds and removing water; and (5) drying the seeds, namely performing forced air drying at the temperature of 20-25 DEG C for 48 hours, and obtaining the treated seeds. The priming of the medicago sativa seeds is carried out by adopting a proper inorganic salt solution, the activity of the seeds and the stress resistance of seedlings can be effectively improved, and the technology is simple and feasible, low in cost and obvious in priming effect and has high production and application values.

The present invention relates to a device and method for classifying seeds, for example coffee seeds. The device is characterised by: a seed-feeding mechanism; a seed-containing mechanism connected to the seed-feeding mechanism; an electronic seed-viewing system operationally disposed in the seed-containing mechanism; a seed-ejecting mechanism connected to the outlet of the seed-containing mechanism, the electronic seed-viewing system having a central processing unit that implements methods for classifying seeds. The method is characterised by the steps of: a) obtaining a digital image of the seed; b) storing the RGB components of the image obtained in step a); c) generating a histogram for each colorimetric and luminosity component of the histogram of step b); d) determining the thresholding point according to Otsu's method; e) obtaining a binary image; f) removing areas; g) obtaining the edges; h) obtaining the vectors corresponding to the seeds; i) identifying black seeds; j) identifying seeds with fermentation- and immaturity-related defects; k) identifying seeds with mechanical damage; and l) actuating a seed-ejecting mechanism, activating actuators for black seeds, actuators for seeds with fermentation- and immaturity-related defects and actuators for seeds with mechanical damage.

Disclosed is a method of priming dry seeds, wherein said seeds firstly wetted in a manner such that the seed absorbs at least 75 wt.% of the amount of water required by the seed for entering phase II of water uptake. Subsequently, the moisture content of the seed is reduced by at least 1 percentage unit, and in manner such that the resulting moisture content of the seed still is at least 25%. At last is the seed incubated in such a manner that: the weight of the seed during the incubation remains at least 80%, such as at least 90% or at least 95%, of the weight of the seed before the incubation; and the moisture content (dry weight based) of the seed during the incubation remains at least 25% during at least 25% of incubation time.