83 results about "Phloretin" patented technology

The invention discloses an application of phloretin in preparation of a medicine or a feed additive for treating or preventing swine envelope virus infection, the medicine is phloretin or oral liquid, granules, tablets and powder prepared from phloretin as an effective component and a solvent or an auxiliary material, and the use dosage is 2.5-10mg / kg of the body weight of a pig; or the phloretin is directly added into the feed, and the addition amount of the phloretin is 0.01-0.03% of the total weight of the pig feed. The phloretin or the composition containing the phloretin is used as a medicine or a feed additive, is high in safety and free of toxic and side effects, and has the effects of remarkably inhibiting replication of envelope viruses such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus and African swine fever virus and reducing the toxicity of the porcine envelope viruses; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome, the morbidity and mortality of the piglets infected with the swine envelope virus are effectively reduced, and the effect of preventing and treating or preventing the swine envelope virus infection diseases is achieved.

The invention discloses application of a plant extract in preparation of a drug for resisting canine coronavirus, and relates to the technical field of biological medicine. Research finds that plant extracts-shikimic acid, phloretin, caffeic acid, tribulus terrestris saponin, berberine hydrochloride and apple polyphenol have activity of resisting canine coronavirus, wherein the effect of phloretin is most remarkable. The phloretin has antiviral activity in three aspects of direct killing, inhibition of virus adsorption and inhibition of virus growth, wherein the effect of inhibiting virus growth is most significant. And the EC50 of the phloretin acting on the CCoV is 0.616 mg / L. The plant extract provided by the invention is derived from natural plants, and has the advantages of low toxicity and good biocompatibility compared with chemical drugs. The invention provides a technical support for developing a novel, effective, safe and side-effect-free medicinal preparation for preventing and treating canine coronavirus.

The invention discloses a composition for resisting porcine viral diarrhea and application of the composition. The composition for resisting porcine viral diarrhea is prepared from the following components in parts by mass: 2 parts of phloretin, 1 part of N-acetyl-D-galactosamine and 100 parts of proline. The composition can further comprise one or more auxiliary materials of absolute ethyl alcohol, propylene glycol, dimethyl sulfoxide, edible oil, water, tween-80, lactose, defatted rice bran, corncob powder, soluble starch, bran, silicon dioxide, montmorillonite, vermiculite, medical stone, zeolite powder, white carbon black and the like. The composition can significantly inhibit the proliferation of PEDV, TGEV and PoRV, and inhibit the infection of PEDV, TGEV and PoRV on cells, and further, the composition can be used for preparing drugs or feed additives for resisting porcine diarrhea diseases caused by viruses such as PEDV, TGEV and PoRV.

The invention discloses a phloretin derivative and application of the phloretin derivative in preparation of a medicine for treating diabetes and complications thereof, and relates to the technical field of small molecule medicines. The structure of the phloretin derivative is shown in the specification. Compared with existing phloretin (unmodified) and a clinically common alpha-glucosidase inhibitor (such as acarbose), the phloretin derivative obtained through structural modification can more efficiently inhibit the activity of alpha-glucosidase, further more effectively delays decomposition and absorption of starch substances, has a better stable regulation effect on postprandial blood sugar, and can be used for preparing the phloretin derivative. The core problem that the existing phloretin inhibitory activity is insufficient is solved.

The invention relates to the technical field of eutectic crystals, in particular to a phloretin and nicotinamide eutectic crystal as well as a preparation method and application thereof, an XRD (X-Ray Diffraction) pattern of the eutectic crystal has characteristic diffraction peaks at least at positions where 2 theta angles are 14.54 degrees, 14.95 degrees, 15.28 degrees, 16.11 degrees, 16.71 degrees, 19.50 degrees, 23.14 degrees, 23.48 degrees, 26.00 degrees, 27.39 degrees and 32.66 degrees, and error tolerance of + / -0.2 degrees exists; in a DSC spectrum, characteristic endothermic peaks exist at 124.3 DEG C, 178.4 DEG C and 283.4 DEG C, and error tolerance of + / -0.2 DEG C exists. The preparation method comprises the following steps: dissolving nicotinamide and phloretin in water, and carrying out ultrasonic treatment, standing, crystallization, filtration, vacuum drying and grinding to obtain the eutectic crystal which can be applied to cosmetics, health care products, food and medicines. The eutectic crystal provided by the invention has the advantages of no irritation, high safety, easy absorption and good stability.

The invention discloses glycosyltransferase in safflower and a related biological material and application thereof. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The HSYA biosynthesis key glycosyl transferase gene UGT708U5 is obtained from safflower for the first time, it is proved that the protein UGT708U5 coded by the gene can catalyze phloretin to form HSYA precursor compounds, namely phloretin-mono-C-glucoside and phloretin-di-C-glucoside, and the gene plays an important role in biosynthesis and heterologous production of HSYA; the method has important theoretical and practical significance for regulating and producing the quinonechalcone C-glycoside compounds of plants and cultivating high-quality safflower carthamus.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses phlorizin glycosylation protease, a gene and application of the phlorizin glycosylation protease. According to the application, glycosyl transferase genes PGGT1.1 and PGGT1.2 for synthesizing phloretin 2 '-O-xylose glucoside through phlorizin glycosylation are obtained, the PGGT1.1 and the PGGT1.2 are over-expressed in plants, and the content of phloretin 2'-O-xylose glucoside in plant leaves is obviously increased, so that biosynthesis, separation and extraction of phloretin 2 '-O-xylose glucoside are facilitated.

The invention discloses a SerpinB3 inhibitor and an application of the SerpinB3 inhibitor. The phloretin can be used as the SerpinB3 inhibitor for the first time, can effectively relieve the symptoms of the rosacea and the psoriasis at the same time, and is used for preparing related drugs for treating inflammatory skin diseases such as the rosacea and the psoriasis. The invention provides a certain theoretical basis for research and development of related treatment products for inflammatory skin diseases such as rosacea or psoriasis.

The invention discloses a method for producing phloretin by using saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. A natural metabolic phloretin path in a plant is successfully constructed in the saccharomyces cerevisiae BY4741, synthesis of phloretin in microorganisms is achieved, synthesis of the phloretin in the microorganisms is enhanced by enhancing synthesis path genes of tyrosine and phenylalanine in the saccharomyces cerevisiae, including elimination of tyrosine feedback inhibition and introduction of exogenous genes, synthesis of prerequisite tyrosine and phenylalanine is enhanced, and the yield of the phloretin is increased. And the yield of the phloretin is further improved by enhancing the expression of a key gene in a phloretin synthesis pathway. The yield of phloretin synthesized from the beginning by the engineering yeast can reach 246.21 mg / L, the synthesis problem of phloretin serving as a carbohydrase inhibitor is effectively solved, and a foundation is laid for synthesizing phloretin through efficient fermentation of microorganisms.

The invention relates to the technical field of biology, in particular to a phloretin-O-monoglucoside glycosyl transferase protein DoUGT019 in dendrobium officinale as well as an encoding gene and application thereof, and discloses a phloretin-O-monoglucoside glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1, which can be used for preparing a glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1 in the presence of a UDP-glucose donor. The method has the advantages that the method is simple and convenient to operate and can catalyze O-glycosylation of phloretin A ring 2 '-OH, 4'-OH and B ring 4-OH, three high-value monoglucosides, namely phlorizin, trilobatin and phloretin-4-O-glucoside, are synchronously generated, multi-product synthesis can be realized without synergy of various enzymes, the process is greatly simplified, and the use cost of enzyme preparations is reduced. Meanwhile, discovery of the protein reveals a unique phloretin glycosylation metabolic pathway of dendrobium plants, and a cloning and functional verification system of the protein can also be used as a general technical template for research of similar glycosyltransferase to promote technical iteration and theoretical development in the field of glycosylation biosynthesis.

The invention discloses application of phloretin in preparation of a medicine for promoting liver cell proliferation, and belongs to the technical field of medicine. A specific cell proliferation tracing system of the hepatocytes is utilized, the proliferation activity of the hepatocytes in an animal body can be continuously recorded, animal experiments find that in the physiological homeostasis maintaining process of a mouse, phloretin can effectively promote the proliferation activity of the hepatocytes in the animal body without affecting the morphological structure of liver tissue, and it is found through hepatic lobule zoning analysis that phloretin can effectively promote the proliferation activity of the hepatocytes in the animal body without affecting the morphological structure of the liver tissue. The phloretin can effectively promote proliferation of hepatic cells in the first, second and third regions of the hepatic lobule. Therefore, phloretin is a potential medicine for liver regeneration.

The invention provides an anti-anemia traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-anemia traditional Chinese medicine composition is prepared from lithocarpus litseifolius, fructus gardeniae and rhizoma gastrodiae. The main active component dihydrochalcone (such as phlorizin, trilobatin, phloretin and the like) of lithocarpus litseifolius can improve iron metabolism by regulating a hepcidin-iron transporter axis; gardenoside in gardenia can target the liver to optimize iron distribution, and gastrodia elata polysaccharide can regulate immunity and improve the marrow microenvironment; the three forms a full-chain regulation and control network of intestinal tract iron absorption, liver iron utilization and bone marrow hematopoiesis microenvironment together, and the anti-anemia effect is enhanced in multiple dimensions.

This invention relates to the field of food preservation technology and discloses a liquid preservative for preserving fresh walnut kernels. The preservative comprises a natural preservative, a solubilizing and stabilizing agent, and a solvent. This liquid preservative uses natural phloretin as the preservative component, combined with Tween 80 as a solubilizing and stabilizing agent and ethanol as a solvent. This effectively solves the problems of poor water and oil solubility and inadequate stability of phloretin, allowing it to be stably dispersed in the preservative system and fully exert its antioxidant and antimicrobial effects. Furthermore, the preparation method is simple and easy to implement, and the production process is safe and controllable. It is convenient to use; the preservation of fresh walnut kernels can be achieved through dilution and soaking. The diluted solution can be reused, reducing usage costs. Simultaneously, it can inhibit bacterial growth during the storage of fresh walnut kernels, delay oxidative deterioration, maintain their good appearance and quality, extend shelf life, and meet the market demand for high-quality fresh walnut kernels.

The invention relates to an SNP (Single Nucleotide Polymorphism) site obviously related to sweet substances of Lithocarpus litseifolius leaves and application of the SNP site. The sweet substances comprise trilobatin, phlorizin, 3-hydroxy phlorizin, neohesperidin dihydrochalcone, phloretin and general flavone. The total number of the SNP loci obviously related to different sweet substances provided by the invention is 84, and the physical positions of the SNP loci are compared and determined according to a reference genome with the login number of GCA040182985.1. The specific SNP site physical position and variation information are shown in a table 5. The loci can be further utilized to establish a molecular marker-assisted selection technology to help breeders to quickly screen high-sweetness Lithocarpus litseifolius strains, so that the traditional breeding period is shortened, and the breeding efficiency is improved. The resource guarantee is provided for the development of a novel Lithocarpus litseifolius sweetener, and the reference is also provided for germplasm innovation, variety specificity identification, quality improvement and genetic evolution relationship discussion of excellent Lithocarpus litseifolius.

The invention relates to a multifunctional ingredient for conditioning meat products, which comprises the following components in terms of 100 kg of raw material meat: 0.2 to 1.2 kg of onion extract, 0.08 to 0.5 kg of garlic extract, 0.008 to 0.8 kg of beet powder, 0.015 to 1.2 kg of cherry powder, 0.008 to 1.2 kg of vinegar powder, 0.08 to 0.8 kg of lemon powder, 50 to 180 g of rice bran extract, 10 to 30 g of mangosteen extract, 30 to 250 g of cinnamon extract, 30 to 120 g of eucommia ulmoides extract and 3 to 20 g of phloretin. 3 to 120g of an enzyme preparation and 0.4 to 120g of bacterial powder. The multifunctional ingredient disclosed by the invention can replace phosphate and / or nitrite, can retain water, prevent corrosion, improve the texture and remove the fishy smell of meat while retaining the characteristic aroma and nutrition of raw meat, has bacteriostatic and antioxidant functions, and can prolong the shelf life of meat products.

The present invention discloses a preparation method of a phyllodium album protein emulsion loaded with phloretin, belonging to the technical field of functional factor delivery. The phyllodium album protein is stirred and dissolved with phosphate buffer solution, and then a magnetic microsphere auxiliary agent with a mass 2-3 times that of the phyllodium album protein is added to obtain a phyllodium album protein aqueous phase; phloretin and linseed oil are stirred and mixed to obtain a phloretin oil phase; the phyllodium album protein aqueous phase and the phloretin oil phase are stirred and mixed according to a volume ratio of 9:1, ultrasonically dispersed after high-speed shearing, and finally the magnetic microsphere auxiliary agent is removed by an external magnetic field separation to obtain a phyllodium album protein emulsion loaded with phloretin. By adding the magnetic microsphere auxiliary agent and combining ultrasonic dispersion treatment, the low water solubility, instability and poor bioavailability of phloretin are improved, so as to prepare a phyllodium album protein emulsion loaded with phloretin with a smaller particle size, good dispersion, good biocompatibility, high stability and good encapsulation effect, which can well improve the solubility and bioavailability of phloretin.

The invention relates to the technical field of apple production place detection, in particular to a red Fuji apple production place discrimination method based on organic component analysis, which comprises the following steps: S1, constructing a linear discrimination function of organic components such as oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin; s2, obtaining the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the apple sample to be detected; s3, inputting the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the obtained apple sample to be detected into the linear discrimination function, and taking a group of maximum discrimination function calculation values as a production area detection result. According to the method, the production places of red Fuji apples in five production areas can be discriminated to a certain extent through linear discrimination established on the basis of eight organic components, the classification accuracy rate reaches 95.2%, and technical support is provided for the production place traceability of the Jinning apples and the quality control of the apples.

The invention relates to a method for detecting the content of puerarin and phloretin in a plant extract, which belongs to the technical field of detection and analysis, and comprises the following steps: sample dissolution, liquid chromatography-tandem mass spectrometry detection and result calculation. According to the method, the content of phloretin and puerarin can be effectively detected, and the limit of quantitation reaches 0.1 ng / mL; according to the present invention, the LC-MSMS spectrum determined by the liquid chromatography-tandem mass spectrometry detection method has characteristics of stable baseline, no drift, strong tandem mass spectrometry detector selectivity and less interference, and has characteristics of good sensitivity, good linear relationship, good precision, good accuracy and good stability when the method is used for determining the phloretin content and the puerarin content, the method has important research value in the aspects of analysis, control research and the like of active ingredients in plant extract raw materials.

The invention discloses emulsifiable paste for treating eczema and a preparation method thereof, and belongs to the technical field of medical application. The preparation method of the emulsifiable paste for treating eczema comprises the following steps: preparing an oil phase: melting caprylic / capric triglyceride, olive oil, beewax and polyglycerol to obtain the oil phase; preparing a water phase: dissolving glycerol, phloretin, acorus calamus extract and zinc acetate in a solvent to obtain the water phase; emulsifying: adding the preheated water phase into the oil phase to form primary emulsion, and then adding beewax for thickening to obtain the cream for treating eczema. The purpose of treating eczema is achieved through transdermal drug delivery of the medicine, the main components are extracted from plants, hormones are not added, toxic and side effects are avoided, and a new choice is provided for clinically treating eczema and relieving various symptoms caused by eczema.

The present invention primarily relates to a mixture comprising or consisting of a) D-allulose and b) a taste modifying compound selected from the group consisting of hesperetin, hersperetindihydrochalcone, phloretin, glycosylated rubusosides, balansines, phyllodulcin, eriodictyol, homoeriodictyol, matairesinol, and mixtures thereof. A further aspect of the present invention relates to a food or beverage product comprising a mixture according to the present invention.

The application relates to an aging corn seed priming agent and a treatment method. The priming agent comprises phloretin and hydroxypropyl-beta-cyclodextrin, and the concentration of the phloretin is 50-200 mg / L. The aging corn seed priming agent provided by the application can significantly improve the germination and seedling growth of aging corn.

The invention relates to a tea seed oil emulsion loaded with grape seed polyphenol-phloretin and a preparation method thereof, and the preparation method comprises the following steps: taking a sucrose-xanthan gum aqueous solution as a water phase, adding grape seed polyphenol into the water phase, taking a polyglycerol polyricinoleate-tea seed oil solution as an oil phase, and uniformly mixing to obtain the tea seed oil emulsion loaded with grape seed polyphenol-phloretin. Grape seed polyphenol can be effectively loaded in an oil phase through a high-speed shearing emulsification method. After the grape seed polyphenol emulsion is prepared, phloretin is added, and the grape seed polyphenol-phloretin loaded tea seed oil emulsion is prepared. According to the method designed by the invention, grape seed polyphenol and phloretin can be stably loaded in an oil solution, so that the problem that water-soluble polyphenol and alcohol-soluble polyphenol are difficult to dissolve in grease is effectively solved; meanwhile, the grape seed polyphenol and the phloretin are compounded for use, so that on one hand, the shelf life of the product can be effectively prolonged, and the oxidation resistance is improved; on the other hand, hydrolysis of alpha-amylase and glucolase on starch can be inhibited, digestion and absorption of carbohydrates are interfered, and powerful technical support is provided for development of novel healthy food.

The invention discloses a hemolysin-like protease inhibition composition for regulating vaginal micro-ecological balance as well as a preparation method and application of the hemolysin-like protease inhibition composition. The hemolysin-like protease inhibition composition disclosed by the invention is prepared from 10 to 20 percent of lactobacillus fermentation liquor, 10 to 30 percent of phloretin, 10 to 30 percent of naringenin, 30 to 40 percent of quercetin and 5 to 10 percent of sodium hyaluronate. The preparation method comprises the following steps: dissolving phloretin, naringenin, quercetin and sodium hyaluronate in 50% butanediol in proportion, and ultrasonically mixing uniformly to obtain a mixed solution; and slowly adding the mixed solution into the lactic acid bacteria fermentation solution, and uniformly stirring to obtain the hemolysin-like protease inhibition composition. According to the invention, natural active components with a hemolysin-like protease inhibition effect are screened to construct a synergistic system with three mechanisms of acidification environment regulation, enzyme activity inhibition and barrier repair, so that hemolysin-like protease can be directly inhibited, and the effect of inhibiting the hemolysin-like protease can be achieved by adjusting the local pH value and the micro-ecological environment of the vagina. Propagation of pathogenic bacteria is inhibited, and growth of dominant flora such as lactobacillus is promoted.

The invention discloses a preparation method of a phloretin-loaded azolla filliculoidas protein emulsion, and belongs to the technical field of functional factor delivery, the method comprises the following steps: stirring and dissolving azolla filliculoidas protein with a phosphate buffer solution, and then adding a magnetic microsphere auxiliary agent with the mass 2-3 times that of the azolla filliculoidas protein to obtain an azolla filliculoidas protein water phase; stirring and mixing phloretin and linseed oil to obtain a phloretin oil phase; the rumex hanus protein water phase and the phloretin oil phase are stirred and mixed according to the volume ratio of 9: 1, ultrasonic dispersion is performed after high-speed shearing, finally, a magnetic field is applied to separate and remove the magnetic microsphere additive, a phloretin-loaded rumex hanus protein emulsion is obtained, and the rumex hanus protein emulsion is prepared by adding the magnetic microsphere additive and combining ultrasonic dispersion treatment. The rumex hanus protein emulsion loaded with the phloretin is prepared by modifying the phloretin and improving the low water solubility, the instability and the poor biological accessibility of the phloretin, so that the rumex hanus protein emulsion loaded with the phloretin, which is small in particle size, good in dispersity, good in biocompatibility, high in stability and good in packaging effect, can be prepared, and the solubility and the biological accessibility of the phloretin can be well improved.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The present invention relates to a cosmetic composition having a skin depigmenting effect which includes, as active ingredient, a combination of apigenin and phloretin, wherein the two components of said combination act synergistically. The invention also relates to the use of the combination of apigenin and phloretin contained in a cosmetic composition for preventing and treating diseases and conditions related to the overproduction or uneven distribution of melanin, wherein said use comprises topically administering an effective quantity of said combination to a patient who needs it, having a synergistic effect.

The present invention provides a freckle-removing and whitening essence and a preparation method thereof, belonging to the technical field of cosmetics. It is prepared from the following raw materials in parts by weight: 10-20 parts of plant extract, 3-5 parts of hirudin / phloretin / ellagic acid chelate complex, 10-20 parts of emollient, 5-10 parts of humectant, 0.3-1 part of thickener, 0.5-3 parts of emulsifier, and 40-60 parts of deionized water. The freckle-removing and whitening essence prepared by the present invention has good effects of whitening, freckle-removing, firming the skin, antioxidant, anti-inflammatory, moisturizing, improving skin elasticity and delaying aging, and has wide sources of raw materials and simple preparation method, and has broad application prospects.

This invention discloses a plant-derived bactericidal composition and its application in fruit juice sterilization. The bactericidal composition consists of two plant-derived compounds with a synergistic effect, including phloretin and dodecylaldehyde, wherein the mass ratio of dodecylaldehyde to phloretin is (1-10):1. At room temperature and pressure, adding the bactericidal composition directly to fruit juice and maintaining it for 2 days can kill the vegetative cells and spores of *Bacillus cyclophosphamide*. This invention is non-toxic, harmless, simple, convenient, green, energy-saving, has high sterilization efficiency, and has no adverse effects on the nutrients and flavor of the fruit juice. This invention is highly effective in solving the problem of spoilage and deterioration of acidic fruit juice beverages caused by incomplete sterilization and spore killing.

The invention relates to the field of medicine application, in particular to phloretin and application thereof. The invention aims to provide an application of phloretin in preparation of a medicine for treating and / or relieving pain in view of obvious adverse reaction of the existing medicine for treating the pain and urgently needing a treatment scheme with a definite treatment effect and no obvious adverse reaction for clinical use in the field.