66 results about "Phloretin" patented technology

The invention discloses a phloretin derivative and application of the phloretin derivative in preparation of a medicine for treating diabetes and complications thereof, and relates to the technical field of small molecule medicines. The structure of the phloretin derivative is shown in the specification. Compared with existing phloretin (unmodified) and a clinically common alpha-glucosidase inhibitor (such as acarbose), the phloretin derivative obtained through structural modification can more efficiently inhibit the activity of alpha-glucosidase, further more effectively delays decomposition and absorption of starch substances, has a better stable regulation effect on postprandial blood sugar, and can be used for preparing the phloretin derivative. The core problem that the existing phloretin inhibitory activity is insufficient is solved.

The invention relates to the technical field of eutectic crystals, in particular to a phloretin and nicotinamide eutectic crystal as well as a preparation method and application thereof, an XRD (X-Ray Diffraction) pattern of the eutectic crystal has characteristic diffraction peaks at least at positions where 2 theta angles are 14.54 degrees, 14.95 degrees, 15.28 degrees, 16.11 degrees, 16.71 degrees, 19.50 degrees, 23.14 degrees, 23.48 degrees, 26.00 degrees, 27.39 degrees and 32.66 degrees, and error tolerance of + / -0.2 degrees exists; in a DSC spectrum, characteristic endothermic peaks exist at 124.3 DEG C, 178.4 DEG C and 283.4 DEG C, and error tolerance of + / -0.2 DEG C exists. The preparation method comprises the following steps: dissolving nicotinamide and phloretin in water, and carrying out ultrasonic treatment, standing, crystallization, filtration, vacuum drying and grinding to obtain the eutectic crystal which can be applied to cosmetics, health care products, food and medicines. The eutectic crystal provided by the invention has the advantages of no irritation, high safety, easy absorption and good stability.

The invention discloses glycosyltransferase in safflower and a related biological material and application thereof. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The HSYA biosynthesis key glycosyl transferase gene UGT708U5 is obtained from safflower for the first time, it is proved that the protein UGT708U5 coded by the gene can catalyze phloretin to form HSYA precursor compounds, namely phloretin-mono-C-glucoside and phloretin-di-C-glucoside, and the gene plays an important role in biosynthesis and heterologous production of HSYA; the method has important theoretical and practical significance for regulating and producing the quinonechalcone C-glycoside compounds of plants and cultivating high-quality safflower carthamus.

The invention discloses a SerpinB3 inhibitor and an application of the SerpinB3 inhibitor. The phloretin can be used as the SerpinB3 inhibitor for the first time, can effectively relieve the symptoms of the rosacea and the psoriasis at the same time, and is used for preparing related drugs for treating inflammatory skin diseases such as the rosacea and the psoriasis. The invention provides a certain theoretical basis for research and development of related treatment products for inflammatory skin diseases such as rosacea or psoriasis.

The invention relates to the technical field of biology, in particular to a phloretin-O-monoglucoside glycosyl transferase protein DoUGT019 in dendrobium officinale as well as an encoding gene and application thereof, and discloses a phloretin-O-monoglucoside glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1, which can be used for preparing a glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1 in the presence of a UDP-glucose donor. The method has the advantages that the method is simple and convenient to operate and can catalyze O-glycosylation of phloretin A ring 2 '-OH, 4'-OH and B ring 4-OH, three high-value monoglucosides, namely phlorizin, trilobatin and phloretin-4-O-glucoside, are synchronously generated, multi-product synthesis can be realized without synergy of various enzymes, the process is greatly simplified, and the use cost of enzyme preparations is reduced. Meanwhile, discovery of the protein reveals a unique phloretin glycosylation metabolic pathway of dendrobium plants, and a cloning and functional verification system of the protein can also be used as a general technical template for research of similar glycosyltransferase to promote technical iteration and theoretical development in the field of glycosylation biosynthesis.

The invention discloses application of phloretin in preparation of a medicine for promoting liver cell proliferation, and belongs to the technical field of medicine. A specific cell proliferation tracing system of the hepatocytes is utilized, the proliferation activity of the hepatocytes in an animal body can be continuously recorded, animal experiments find that in the physiological homeostasis maintaining process of a mouse, phloretin can effectively promote the proliferation activity of the hepatocytes in the animal body without affecting the morphological structure of liver tissue, and it is found through hepatic lobule zoning analysis that phloretin can effectively promote the proliferation activity of the hepatocytes in the animal body without affecting the morphological structure of the liver tissue. The phloretin can effectively promote proliferation of hepatic cells in the first, second and third regions of the hepatic lobule. Therefore, phloretin is a potential medicine for liver regeneration.

The invention provides an anti-anemia traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-anemia traditional Chinese medicine composition is prepared from lithocarpus litseifolius, fructus gardeniae and rhizoma gastrodiae. The main active component dihydrochalcone (such as phlorizin, trilobatin, phloretin and the like) of lithocarpus litseifolius can improve iron metabolism by regulating a hepcidin-iron transporter axis; gardenoside in gardenia can target the liver to optimize iron distribution, and gastrodia elata polysaccharide can regulate immunity and improve the marrow microenvironment; the three forms a full-chain regulation and control network of intestinal tract iron absorption, liver iron utilization and bone marrow hematopoiesis microenvironment together, and the anti-anemia effect is enhanced in multiple dimensions.

This invention relates to the field of food preservation technology and discloses a liquid preservative for preserving fresh walnut kernels. The preservative comprises a natural preservative, a solubilizing and stabilizing agent, and a solvent. This liquid preservative uses natural phloretin as the preservative component, combined with Tween 80 as a solubilizing and stabilizing agent and ethanol as a solvent. This effectively solves the problems of poor water and oil solubility and inadequate stability of phloretin, allowing it to be stably dispersed in the preservative system and fully exert its antioxidant and antimicrobial effects. Furthermore, the preparation method is simple and easy to implement, and the production process is safe and controllable. It is convenient to use; the preservation of fresh walnut kernels can be achieved through dilution and soaking. The diluted solution can be reused, reducing usage costs. Simultaneously, it can inhibit bacterial growth during the storage of fresh walnut kernels, delay oxidative deterioration, maintain their good appearance and quality, extend shelf life, and meet the market demand for high-quality fresh walnut kernels.

The invention relates to an SNP (Single Nucleotide Polymorphism) site obviously related to sweet substances of Lithocarpus litseifolius leaves and application of the SNP site. The sweet substances comprise trilobatin, phlorizin, 3-hydroxy phlorizin, neohesperidin dihydrochalcone, phloretin and general flavone. The total number of the SNP loci obviously related to different sweet substances provided by the invention is 84, and the physical positions of the SNP loci are compared and determined according to a reference genome with the login number of GCA040182985.1. The specific SNP site physical position and variation information are shown in a table 5. The loci can be further utilized to establish a molecular marker-assisted selection technology to help breeders to quickly screen high-sweetness Lithocarpus litseifolius strains, so that the traditional breeding period is shortened, and the breeding efficiency is improved. The resource guarantee is provided for the development of a novel Lithocarpus litseifolius sweetener, and the reference is also provided for germplasm innovation, variety specificity identification, quality improvement and genetic evolution relationship discussion of excellent Lithocarpus litseifolius.

The invention relates to a multifunctional ingredient for conditioning meat products, which comprises the following components in terms of 100 kg of raw material meat: 0.2 to 1.2 kg of onion extract, 0.08 to 0.5 kg of garlic extract, 0.008 to 0.8 kg of beet powder, 0.015 to 1.2 kg of cherry powder, 0.008 to 1.2 kg of vinegar powder, 0.08 to 0.8 kg of lemon powder, 50 to 180 g of rice bran extract, 10 to 30 g of mangosteen extract, 30 to 250 g of cinnamon extract, 30 to 120 g of eucommia ulmoides extract and 3 to 20 g of phloretin. 3 to 120g of an enzyme preparation and 0.4 to 120g of bacterial powder. The multifunctional ingredient disclosed by the invention can replace phosphate and / or nitrite, can retain water, prevent corrosion, improve the texture and remove the fishy smell of meat while retaining the characteristic aroma and nutrition of raw meat, has bacteriostatic and antioxidant functions, and can prolong the shelf life of meat products.

The present invention discloses a preparation method of a phyllodium album protein emulsion loaded with phloretin, belonging to the technical field of functional factor delivery. The phyllodium album protein is stirred and dissolved with phosphate buffer solution, and then a magnetic microsphere auxiliary agent with a mass 2-3 times that of the phyllodium album protein is added to obtain a phyllodium album protein aqueous phase; phloretin and linseed oil are stirred and mixed to obtain a phloretin oil phase; the phyllodium album protein aqueous phase and the phloretin oil phase are stirred and mixed according to a volume ratio of 9:1, ultrasonically dispersed after high-speed shearing, and finally the magnetic microsphere auxiliary agent is removed by an external magnetic field separation to obtain a phyllodium album protein emulsion loaded with phloretin. By adding the magnetic microsphere auxiliary agent and combining ultrasonic dispersion treatment, the low water solubility, instability and poor bioavailability of phloretin are improved, so as to prepare a phyllodium album protein emulsion loaded with phloretin with a smaller particle size, good dispersion, good biocompatibility, high stability and good encapsulation effect, which can well improve the solubility and bioavailability of phloretin.

The invention relates to the technical field of apple production place detection, in particular to a red Fuji apple production place discrimination method based on organic component analysis, which comprises the following steps: S1, constructing a linear discrimination function of organic components such as oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin; s2, obtaining the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the apple sample to be detected; s3, inputting the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the obtained apple sample to be detected into the linear discrimination function, and taking a group of maximum discrimination function calculation values as a production area detection result. According to the method, the production places of red Fuji apples in five production areas can be discriminated to a certain extent through linear discrimination established on the basis of eight organic components, the classification accuracy rate reaches 95.2%, and technical support is provided for the production place traceability of the Jinning apples and the quality control of the apples.

The invention discloses emulsifiable paste for treating eczema and a preparation method thereof, and belongs to the technical field of medical application. The preparation method of the emulsifiable paste for treating eczema comprises the following steps: preparing an oil phase: melting caprylic / capric triglyceride, olive oil, beewax and polyglycerol to obtain the oil phase; preparing a water phase: dissolving glycerol, phloretin, acorus calamus extract and zinc acetate in a solvent to obtain the water phase; emulsifying: adding the preheated water phase into the oil phase to form primary emulsion, and then adding beewax for thickening to obtain the cream for treating eczema. The purpose of treating eczema is achieved through transdermal drug delivery of the medicine, the main components are extracted from plants, hormones are not added, toxic and side effects are avoided, and a new choice is provided for clinically treating eczema and relieving various symptoms caused by eczema.

The present invention primarily relates to a mixture comprising or consisting of a) D-allulose and b) a taste modifying compound selected from the group consisting of hesperetin, hersperetindihydrochalcone, phloretin, glycosylated rubusosides, balansines, phyllodulcin, eriodictyol, homoeriodictyol, matairesinol, and mixtures thereof. A further aspect of the present invention relates to a food or beverage product comprising a mixture according to the present invention.

The application relates to an aging corn seed priming agent and a treatment method. The priming agent comprises phloretin and hydroxypropyl-beta-cyclodextrin, and the concentration of the phloretin is 50-200 mg / L. The aging corn seed priming agent provided by the application can significantly improve the germination and seedling growth of aging corn.

The invention discloses a hemolysin-like protease inhibition composition for regulating vaginal micro-ecological balance as well as a preparation method and application of the hemolysin-like protease inhibition composition. The hemolysin-like protease inhibition composition disclosed by the invention is prepared from 10 to 20 percent of lactobacillus fermentation liquor, 10 to 30 percent of phloretin, 10 to 30 percent of naringenin, 30 to 40 percent of quercetin and 5 to 10 percent of sodium hyaluronate. The preparation method comprises the following steps: dissolving phloretin, naringenin, quercetin and sodium hyaluronate in 50% butanediol in proportion, and ultrasonically mixing uniformly to obtain a mixed solution; and slowly adding the mixed solution into the lactic acid bacteria fermentation solution, and uniformly stirring to obtain the hemolysin-like protease inhibition composition. According to the invention, natural active components with a hemolysin-like protease inhibition effect are screened to construct a synergistic system with three mechanisms of acidification environment regulation, enzyme activity inhibition and barrier repair, so that hemolysin-like protease can be directly inhibited, and the effect of inhibiting the hemolysin-like protease can be achieved by adjusting the local pH value and the micro-ecological environment of the vagina. Propagation of pathogenic bacteria is inhibited, and growth of dominant flora such as lactobacillus is promoted.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The present invention provides a freckle-removing and whitening essence and a preparation method thereof, belonging to the technical field of cosmetics. It is prepared from the following raw materials in parts by weight: 10-20 parts of plant extract, 3-5 parts of hirudin / phloretin / ellagic acid chelate complex, 10-20 parts of emollient, 5-10 parts of humectant, 0.3-1 part of thickener, 0.5-3 parts of emulsifier, and 40-60 parts of deionized water. The freckle-removing and whitening essence prepared by the present invention has good effects of whitening, freckle-removing, firming the skin, antioxidant, anti-inflammatory, moisturizing, improving skin elasticity and delaying aging, and has wide sources of raw materials and simple preparation method, and has broad application prospects.

This invention discloses a plant-derived bactericidal composition and its application in fruit juice sterilization. The bactericidal composition consists of two plant-derived compounds with a synergistic effect, including phloretin and dodecylaldehyde, wherein the mass ratio of dodecylaldehyde to phloretin is (1-10):1. At room temperature and pressure, adding the bactericidal composition directly to fruit juice and maintaining it for 2 days can kill the vegetative cells and spores of *Bacillus cyclophosphamide*. This invention is non-toxic, harmless, simple, convenient, green, energy-saving, has high sterilization efficiency, and has no adverse effects on the nutrients and flavor of the fruit juice. This invention is highly effective in solving the problem of spoilage and deterioration of acidic fruit juice beverages caused by incomplete sterilization and spore killing.

The invention relates to the field of medicine application, in particular to phloretin and application thereof. The invention aims to provide an application of phloretin in preparation of a medicine for treating and / or relieving pain in view of obvious adverse reaction of the existing medicine for treating the pain and urgently needing a treatment scheme with a definite treatment effect and no obvious adverse reaction for clinical use in the field.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The invention provides a C-glycosyl transferase mutant Mut10 with remarkably improved thermal stability and application of the C-glycosyl transferase mutant Mut10. Specifically, a mutant Mut10 of which the thermal stability is greatly improved is obtained by introducing ten-site combined mutation of S155A, V194D, G200Y, L227E, K381P, D411G, G424A, V437R, G456S and C457Q into C-glycosyl transferase GgCGT (GenBank ID: MH998596) from Glycyrrhiza glabra. The invention also comprises a method for producing the heat-resistant C-glycosyl transferase mutant, and an application of the heat-resistant C-glycosyl transferase mutant in catalytic synthesis of phloretin di-Cglucoside.

The application relates to the field of biological medicine, and particularly discloses a compound, a composition and application of the compound and the composition in preparation of a medicine for treating arrhythmia. Research shows that compounds such as cassia anthraquinone, phloretin and primuletin have the effect of treating arrhythmia; therefore, the cassia anthraquinone, the phloretin or the primuletin or a combination thereof is used as an effective component for preparing the medicine for treating arrhythmia, which has important application value.

The present invention discloses a mutant of Erigeron breviscapus with enhanced chalcone synthase activity and / or selectivity, and a screening method and application thereof. p The invention relates to a recombinant plasmid for synthesizing phloretin using ‑coumaric acid as a substrate. Through structure prediction and catalytic mechanism, sites related to the catalytic activity and selectivity of EbCHS are screened, and effective mutants and effective mutation sites are obtained through experimental screening. EbCHS is compared with different CHS sequences, and screening experiments are performed to obtain effective mutants of non-conserved amino acid sites adjacent to the above-mentioned effective mutation sites. The two obtained effective mutants are combined in pairs to obtain relevant detection information. Based on the above-mentioned experimental information, a database is built and machine learning modeling is predicted. After experimental verification, EbCHS with significantly enhanced activity and selectivity in the phloretin synthesis pathway is obtained, which can be used as p ‑coumaric acid as a substrate for efficient synthesis of phloretin and reduced by-products, providing technical support for promoting the industrial production of phloretin.

The invention discloses application of a plant extract in preparation of a medicine for inhibiting canine parvovirus, and relates to the technical field of biological medicine. The plant extract is at least one of phloretin, neohesperidin-dihydrochalcone, tribulus terrestris saponin, rubusoside, soy isoflavone, berberine hydrochloride, dioscin and silymarin. In-depth study on various plant extracts shows that phloretin, neohesperidin-dihydrochalcone, tribulus terrestris saponin, rubusoside, soy isoflavone, berberine hydrochloride, dioscin and silymarin all show a remarkable canine parvovirus inhibition effect, and the effect of soy isoflavone is particularly outstanding. The research result provides a brand new natural medicine choice for preventing and treating canine parvovirus. The application range of natural plant extracts in the field of veterinary medicine is expanded, and important technical support is provided for developing novel, efficient and safe canine parvovirus prevention and treatment medicine preparations without side effects.

The invention belongs to the technical field of biology, and discloses a method for producing phloretin through fermentation of eurotium cristatum. On the basis of pretreatment of lithocarpus litseifolius, eurotium cristatum is used as a fermentation inoculant, and after aerobic solid fermentation is performed on the eurotium cristatum, trilobatin and phlorizin in the lithocarpus litseifolius are converted into phloretin. According to the method, eurotium cristatum is inoculated into lithocarpus litseifolius by utilizing a microbial solid-state fermentation technology, high-content phloretin is obtained after fermentation is finished, and the method is simple, convenient, short in production period and suitable for subsequent large-scale production.

The invention discloses a phloretin solution for poultry. The phloretin solution is prepared from the following components in parts by mass: 1 part of phloretin, 20 to 35 parts of ethanol serving as a solvent, 2.5 to 7.5 parts of polysorbate 80 serving as a cosolvent, 0.008 to 0.2 part of butylated hydroxytoluene serving as an antioxidant, 0.01 to 0.05 part of phosphoric acid serving as a synergist serving as an antioxidant and 5 to 20 parts of purified water. The preparation method comprises the following steps: dissolving phloretin and butylated hydroxytoluene in ethanol, adding polysorbate 80 and phosphoric acid, adding water to a constant volume, and filtering. The problem that phloretin is easy to separate out when being mixed with water to be administered is solved, the solution stability is high, accurate administration can be achieved, the composition is suitable for preventing necrotic enteritis caused by clostridium welchii in poultry intensive breeding, and the policy requirements of resisting limitation and resisting replacement are met.

The application discloses a meat-flavor seasoning for instant food and a preparation method thereof, and belongs to the technical field of food seasoning processing. The application forms a dispersion liquid by dispersing bovine bone powder in water, sequentially performs enzymolysis on the dispersion liquid by using neutral protease and trypsin, and collects supernatant by centrifugation; reduces sugar, L-cysteine, vitamin B1, ferulic acid and phloretin are added into the supernatant, and then Maillard reaction is performed; finally, the melted bovine fat is emulsified; and finally, the liquid, paste or powder seasoning is prepared. Compared with the prior art, the application fully releases bovine bone protein and converts the bovine bone protein into flavor precursors in a directional manner, the meat flavor is rich, the fat flavor is coordinated, the umami is full, the flavor stability is good, and the application is suitable for instant food soup and seasoning systems.

This invention belongs to the field of pharmacology and provides the application of demethoxycurcumin and phlorizin in the preparation of drugs that inhibit β-lactoglobulin glycosylation. The synergistic inhibitory effects and mechanisms of demethoxycurcumin and phlorizin on AGEs were systematically investigated. Fluorescence spectroscopy and carbonyl / thiol / amino group assays confirmed that demethoxycurcumin and phlorizin can synergistically inhibit β-lactoglobulin glycosylation at appropriate concentrations. Circular dichroism spectroscopy and other experiments showed that the combined use of the two can better alleviate protein secondary structure changes and aggregation. They competitively bind to the same glycosylation site, synergistically resist oxidation, thereby inhibiting AGEs formation, and synergistically reducing oxidative stress, cell damage, and apoptosis at the cellular level. Therefore, the combined application of these two drugs has potential therapeutic value in intervening in diabetic complications.