45 results about "Phloretin" patented technology

The invention discloses a phloretin derivative and application of the phloretin derivative in preparation of a medicine for treating diabetes and complications thereof, and relates to the technical field of small molecule medicines. The structure of the phloretin derivative is shown in the specification. Compared with existing phloretin (unmodified) and a clinically common alpha-glucosidase inhibitor (such as acarbose), the phloretin derivative obtained through structural modification can more efficiently inhibit the activity of alpha-glucosidase, further more effectively delays decomposition and absorption of starch substances, has a better stable regulation effect on postprandial blood sugar, and can be used for preparing the phloretin derivative. The core problem that the existing phloretin inhibitory activity is insufficient is solved.

The invention discloses a SerpinB3 inhibitor and an application of the SerpinB3 inhibitor. The phloretin can be used as the SerpinB3 inhibitor for the first time, can effectively relieve the symptoms of the rosacea and the psoriasis at the same time, and is used for preparing related drugs for treating inflammatory skin diseases such as the rosacea and the psoriasis. The invention provides a certain theoretical basis for research and development of related treatment products for inflammatory skin diseases such as rosacea or psoriasis.

The invention relates to the technical field of biology, in particular to a phloretin-O-monoglucoside glycosyl transferase protein DoUGT019 in dendrobium officinale as well as an encoding gene and application thereof, and discloses a phloretin-O-monoglucoside glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1, which can be used for preparing a glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1 in the presence of a UDP-glucose donor. The method has the advantages that the method is simple and convenient to operate and can catalyze O-glycosylation of phloretin A ring 2 '-OH, 4'-OH and B ring 4-OH, three high-value monoglucosides, namely phlorizin, trilobatin and phloretin-4-O-glucoside, are synchronously generated, multi-product synthesis can be realized without synergy of various enzymes, the process is greatly simplified, and the use cost of enzyme preparations is reduced. Meanwhile, discovery of the protein reveals a unique phloretin glycosylation metabolic pathway of dendrobium plants, and a cloning and functional verification system of the protein can also be used as a general technical template for research of similar glycosyltransferase to promote technical iteration and theoretical development in the field of glycosylation biosynthesis.

The invention provides an anti-anemia traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-anemia traditional Chinese medicine composition is prepared from lithocarpus litseifolius, fructus gardeniae and rhizoma gastrodiae. The main active component dihydrochalcone (such as phlorizin, trilobatin, phloretin and the like) of lithocarpus litseifolius can improve iron metabolism by regulating a hepcidin-iron transporter axis; gardenoside in gardenia can target the liver to optimize iron distribution, and gastrodia elata polysaccharide can regulate immunity and improve the marrow microenvironment; the three forms a full-chain regulation and control network of intestinal tract iron absorption, liver iron utilization and bone marrow hematopoiesis microenvironment together, and the anti-anemia effect is enhanced in multiple dimensions.

This invention relates to the field of food preservation technology and discloses a liquid preservative for preserving fresh walnut kernels. The preservative comprises a natural preservative, a solubilizing and stabilizing agent, and a solvent. This liquid preservative uses natural phloretin as the preservative component, combined with Tween 80 as a solubilizing and stabilizing agent and ethanol as a solvent. This effectively solves the problems of poor water and oil solubility and inadequate stability of phloretin, allowing it to be stably dispersed in the preservative system and fully exert its antioxidant and antimicrobial effects. Furthermore, the preparation method is simple and easy to implement, and the production process is safe and controllable. It is convenient to use; the preservation of fresh walnut kernels can be achieved through dilution and soaking. The diluted solution can be reused, reducing usage costs. Simultaneously, it can inhibit bacterial growth during the storage of fresh walnut kernels, delay oxidative deterioration, maintain their good appearance and quality, extend shelf life, and meet the market demand for high-quality fresh walnut kernels.

The invention relates to the technical field of apple production place detection, in particular to a red Fuji apple production place discrimination method based on organic component analysis, which comprises the following steps: S1, constructing a linear discrimination function of organic components such as oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin; s2, obtaining the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the apple sample to be detected; s3, inputting the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the obtained apple sample to be detected into the linear discrimination function, and taking a group of maximum discrimination function calculation values as a production area detection result. According to the method, the production places of red Fuji apples in five production areas can be discriminated to a certain extent through linear discrimination established on the basis of eight organic components, the classification accuracy rate reaches 95.2%, and technical support is provided for the production place traceability of the Jinning apples and the quality control of the apples.

The invention discloses emulsifiable paste for treating eczema and a preparation method thereof, and belongs to the technical field of medical application. The preparation method of the emulsifiable paste for treating eczema comprises the following steps: preparing an oil phase: melting caprylic / capric triglyceride, olive oil, beewax and polyglycerol to obtain the oil phase; preparing a water phase: dissolving glycerol, phloretin, acorus calamus extract and zinc acetate in a solvent to obtain the water phase; emulsifying: adding the preheated water phase into the oil phase to form primary emulsion, and then adding beewax for thickening to obtain the cream for treating eczema. The purpose of treating eczema is achieved through transdermal drug delivery of the medicine, the main components are extracted from plants, hormones are not added, toxic and side effects are avoided, and a new choice is provided for clinically treating eczema and relieving various symptoms caused by eczema.

The application relates to an aging corn seed priming agent and a treatment method. The priming agent comprises phloretin and hydroxypropyl-beta-cyclodextrin, and the concentration of the phloretin is 50-200 mg / L. The aging corn seed priming agent provided by the application can significantly improve the germination and seedling growth of aging corn.

The invention discloses a hemolysin-like protease inhibition composition for regulating vaginal micro-ecological balance as well as a preparation method and application of the hemolysin-like protease inhibition composition. The hemolysin-like protease inhibition composition disclosed by the invention is prepared from 10 to 20 percent of lactobacillus fermentation liquor, 10 to 30 percent of phloretin, 10 to 30 percent of naringenin, 30 to 40 percent of quercetin and 5 to 10 percent of sodium hyaluronate. The preparation method comprises the following steps: dissolving phloretin, naringenin, quercetin and sodium hyaluronate in 50% butanediol in proportion, and ultrasonically mixing uniformly to obtain a mixed solution; and slowly adding the mixed solution into the lactic acid bacteria fermentation solution, and uniformly stirring to obtain the hemolysin-like protease inhibition composition. According to the invention, natural active components with a hemolysin-like protease inhibition effect are screened to construct a synergistic system with three mechanisms of acidification environment regulation, enzyme activity inhibition and barrier repair, so that hemolysin-like protease can be directly inhibited, and the effect of inhibiting the hemolysin-like protease can be achieved by adjusting the local pH value and the micro-ecological environment of the vagina. Propagation of pathogenic bacteria is inhibited, and growth of dominant flora such as lactobacillus is promoted.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

This invention discloses a plant-derived bactericidal composition and its application in fruit juice sterilization. The bactericidal composition consists of two plant-derived compounds with a synergistic effect, including phloretin and dodecylaldehyde, wherein the mass ratio of dodecylaldehyde to phloretin is (1-10):1. At room temperature and pressure, adding the bactericidal composition directly to fruit juice and maintaining it for 2 days can kill the vegetative cells and spores of *Bacillus cyclophosphamide*. This invention is non-toxic, harmless, simple, convenient, green, energy-saving, has high sterilization efficiency, and has no adverse effects on the nutrients and flavor of the fruit juice. This invention is highly effective in solving the problem of spoilage and deterioration of acidic fruit juice beverages caused by incomplete sterilization and spore killing.

The invention relates to the field of medicine application, in particular to phloretin and application thereof. The invention aims to provide an application of phloretin in preparation of a medicine for treating and / or relieving pain in view of obvious adverse reaction of the existing medicine for treating the pain and urgently needing a treatment scheme with a definite treatment effect and no obvious adverse reaction for clinical use in the field.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The invention provides a C-glycosyl transferase mutant Mut10 with remarkably improved thermal stability and application of the C-glycosyl transferase mutant Mut10. Specifically, a mutant Mut10 of which the thermal stability is greatly improved is obtained by introducing ten-site combined mutation of S155A, V194D, G200Y, L227E, K381P, D411G, G424A, V437R, G456S and C457Q into C-glycosyl transferase GgCGT (GenBank ID: MH998596) from Glycyrrhiza glabra. The invention also comprises a method for producing the heat-resistant C-glycosyl transferase mutant, and an application of the heat-resistant C-glycosyl transferase mutant in catalytic synthesis of phloretin di-Cglucoside.

The application relates to the field of biological medicine, and particularly discloses a compound, a composition and application of the compound and the composition in preparation of a medicine for treating arrhythmia. Research shows that compounds such as cassia anthraquinone, phloretin and primuletin have the effect of treating arrhythmia; therefore, the cassia anthraquinone, the phloretin or the primuletin or a combination thereof is used as an effective component for preparing the medicine for treating arrhythmia, which has important application value.

The invention belongs to the technical field of biology, and discloses a method for producing phloretin through fermentation of eurotium cristatum. On the basis of pretreatment of lithocarpus litseifolius, eurotium cristatum is used as a fermentation inoculant, and after aerobic solid fermentation is performed on the eurotium cristatum, trilobatin and phlorizin in the lithocarpus litseifolius are converted into phloretin. According to the method, eurotium cristatum is inoculated into lithocarpus litseifolius by utilizing a microbial solid-state fermentation technology, high-content phloretin is obtained after fermentation is finished, and the method is simple, convenient, short in production period and suitable for subsequent large-scale production.

The invention discloses a phloretin solution for poultry. The phloretin solution is prepared from the following components in parts by mass: 1 part of phloretin, 20 to 35 parts of ethanol serving as a solvent, 2.5 to 7.5 parts of polysorbate 80 serving as a cosolvent, 0.008 to 0.2 part of butylated hydroxytoluene serving as an antioxidant, 0.01 to 0.05 part of phosphoric acid serving as a synergist serving as an antioxidant and 5 to 20 parts of purified water. The preparation method comprises the following steps: dissolving phloretin and butylated hydroxytoluene in ethanol, adding polysorbate 80 and phosphoric acid, adding water to a constant volume, and filtering. The problem that phloretin is easy to separate out when being mixed with water to be administered is solved, the solution stability is high, accurate administration can be achieved, the composition is suitable for preventing necrotic enteritis caused by clostridium welchii in poultry intensive breeding, and the policy requirements of resisting limitation and resisting replacement are met.

The application discloses a meat-flavor seasoning for instant food and a preparation method thereof, and belongs to the technical field of food seasoning processing. The application forms a dispersion liquid by dispersing bovine bone powder in water, sequentially performs enzymolysis on the dispersion liquid by using neutral protease and trypsin, and collects supernatant by centrifugation; reduces sugar, L-cysteine, vitamin B1, ferulic acid and phloretin are added into the supernatant, and then Maillard reaction is performed; finally, the melted bovine fat is emulsified; and finally, the liquid, paste or powder seasoning is prepared. Compared with the prior art, the application fully releases bovine bone protein and converts the bovine bone protein into flavor precursors in a directional manner, the meat flavor is rich, the fat flavor is coordinated, the umami is full, the flavor stability is good, and the application is suitable for instant food soup and seasoning systems.

This invention belongs to the field of pharmacology and provides the application of demethoxycurcumin and phlorizin in the preparation of drugs that inhibit β-lactoglobulin glycosylation. The synergistic inhibitory effects and mechanisms of demethoxycurcumin and phlorizin on AGEs were systematically investigated. Fluorescence spectroscopy and carbonyl / thiol / amino group assays confirmed that demethoxycurcumin and phlorizin can synergistically inhibit β-lactoglobulin glycosylation at appropriate concentrations. Circular dichroism spectroscopy and other experiments showed that the combined use of the two can better alleviate protein secondary structure changes and aggregation. They competitively bind to the same glycosylation site, synergistically resist oxidation, thereby inhibiting AGEs formation, and synergistically reducing oxidative stress, cell damage, and apoptosis at the cellular level. Therefore, the combined application of these two drugs has potential therapeutic value in intervening in diabetic complications.

The invention discloses a protein related to phloretin enzyme activity as well as a coding gene and application thereof. The technical problem to be solved is how to provide a phloretin enzyme activity related protein. The invention specifically discloses any one of the following applications: A1) application of the protein in catalyzing phloretin to generate phlorizin and / or trilobatin and / or preparing a product for catalyzing phloretin to generate phlorizin and / or trilobatin; a2) application in production of phlorizin and / or preparation of a product for producing phlorizin; and A3) producing trilobatin and / or preparing a product for producing the trilobatin. The protein is any one of the following: B1) a protein with an amino acid sequence as shown in a sequence 2; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The method can be used for industrial production.

This invention relates to a composition containing protoplasts of ginseng as an active ingredient, which improves aging caused by the deepening of wrinkles or loss of firmness in the skin. Specifically, the present invention contains protoplasts of ginseng as an active ingredient and can improve aging caused by deep wrinkles or loss of firmness of the skin. Furthermore, by including one or more components selected from the group consisting of phloretin, oryzanol, tranexamic acid, and pyridoxine, the stability of the composition can be enhanced and the effect of improving skin wrinkles and firmness can be strengthened by suppressing the clumping or cell membrane defects of the ginseng protoplasts in the composition.

The application discloses a method for simultaneously determining contents of advantame, phloretin and perillartine in edible essence based on UPLC, and belongs to the technical field of chemical analysis and detection, and comprises preparation of three sweetener series standard solutions, preparation of a to-be-measured sample solution, and subsequent detection and quantitative analysis by means of an ultra-high performance liquid chromatograph, and is suitable for simultaneous detection of the three sweeteners advantame, phloretin and perillartine. Compared with the prior art, different detection methods need to be adopted for detection of the three sweeteners, and the method can effectively separate and quantitatively analyze the three sweeteners with different absorption wavelengths at one time, and is rapid and convenient to operate, high in detection efficiency and easy to popularize.

The application provides a novel method for preparing a phloretin derivative. The method comprises modifying and grafting a hydroxyl group in p-hydroxyphenylpropionic acid in a raw material for synthesizing phloretin in advance, and then performing a synthesis reaction with phloroglucinol, i.e. m-benzene triol, to obtain a corresponding phloretin derivative, i.e. tetraacetyl glucoside modified phloretin. The derivative retains the antioxidant and anti-inflammatory properties of phloretin, and has good water solubility and skin absorption efficiency. Therefore, the obtained phloretin derivative can be widely applied to various forms of cosmetic products, and has excellent antioxidant and anti-inflammatory effects.

The present invention discloses a skin care composition, comprising a first active component and a second auxiliary component. The first active component is selected from one or more of tetrahydrocurcumin, ergothioneine, phloretin, arbutin, resveratrol, vitamin C, dipotassium glycyrrhizinate, ceramide, Aloe vera polysaccharide, Opuntia dillenii polysaccharide, Astragali radix polysaccharide, Dendrobii caulis polysaccharide, Cucumis metuliferus polysaccharide, fucoidan, small-molecule hyaluronic acid, pearl powder, collagen, tea tree essential oil, thearubigins, salicylic acid, retinol, glycoxyl, carnosine; the second auxiliary component is selected from one or more of tetrahydropiperine, laurocapram, PEG-75 shea butter glycerides, hydrogenated castor oil, glycerol, lauric acid, sucrose laurate, butanediol, polysorbate, hydroxyethyl cellulose, 1,2-pentanediol, ethoxydiglycol. The skin care composition of the present invention can be used for whitening, anti-inflammatory, anti-glycation, moisturizing, skin firming, anti-bacterial and / or anti-aging.

The invention discloses a process method for extracting trilobatin from sweet tea tender leaves, and belongs to the technical field of trilobatin separation. The method comprises the following steps: countercurrent extraction, chromatographic separation and crystallization purification, and specifically comprises the following steps: by taking tender leaves of strigose hydrangea juvenile leaves as a raw material, treating the tender leaves of strigose hydrangea juvenile leaves by adopting an aqueous solution of mixed enzyme and a surfactant, and countercurrent extraction, so that the solubility of trilobatin in water is improved, the trilobatin is fully dissolved out, and the use of a large amount of organic extraction solvents is avoided; and based on the molecular sieve action of macroporous resin and the principle that the solubility of trilobatin in a low-temperature aqueous solution is reduced, the removal of components such as polysaccharide, protein and phloretin in the sweet tea tender leaves is realized, and high-purity trilobatin crystals are obtained. The method has the advantages of simple and efficient process, no need of specific equipment and suitability for industrial production, and has a wide application prospect in the field of trilobatin extraction.

The invention relates to a combination containing an extract of Coleus forskohlii, an apple extract rich in proanthocyanidins, an extract of grape pomace and a phloretin powder.

The application discloses application of phloretin in preparation of a medicine or a feed additive for treating or preventing porcine capsid virus infection, the medicine being phloretin or oral liquid, granules, tablets, powder prepared by adding a solvent or excipient with phloretin as an effective component, and a dosage being 2.5-10 mg / kg of pig body weight; or the phloretin being directly added into feed, and an adding amount being 0.01%-0.03% of the total weight of pig feed. The application takes phloretin or a composition containing phloretin as the medicine or the feed additive, is high in safety, free of toxic side effects, has a significant effect of inhibiting replication of capsid viruses such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus and African swine fever virus, reducing the virulence of porcine capsid virus, overcoming the disadvantages of possible immunization failure of vaccination and virus variation caused by use of antiviral drugs, effectively reducing the morbidity and mortality of piglets infected with porcine capsid virus, and having the effect of preventing and treating or preventing porcine capsid virus infection diseases.

The invention provides a phloretin-trilobatin co-amorphous substance and a solid dispersion thereof, and a preparation method and pharmaceutical application thereof. The two active ingredients of phloretin and trilobatin can be directly prepared into an amorphous substance under the condition of not using auxiliary materials, so that the solubility of phloretin and trilobatin is improved. On the basis, micromolecular sugar alcohol is further introduced, the solid dispersion is prepared under the condition that other auxiliary materials are not added, and the solubility of the two is more remarkably improved. The preparation process is simple, efficient, low in cost and easy to industrialize. The obtained product not only can enhance the water solubility of phloretin and trilobatin at the same time, has a better sweetener application prospect, but also shows good in-vitro blood fat reducing activity, and the phloretin and the trilobatin show a remarkable synergistic effect, so that a new strategy is provided for developing an efficient compound blood fat reducing preparation.

The invention discloses a formula for preventing and treating oral inflammation and a preparation method thereof, and relates to the technical field of formulas, the formula comprises the following components by weight: 90-100 parts of NHDC; 2 to 5 parts of proline; 0.5 to 1.5 parts of hesperetin; and 0.5 to 1.5 parts of phloretin. The preparation method comprises the following steps: preparing a certain amount of water, heating to 70-90 DEG C, stirring and dissolving NHDC, sequentially adding phloretin, hesperetin and proline, stirring for 2-4 hours, concentrating until the relative density is 1.0-1.5, and spray-drying to obtain the cocrystal structure. The formula provided by the invention is simple in components, low in cost and simple in preparation process; through a special preparation process, NHDC, proline, hesperetin and phloretin form a co-crystal structure, and the pharmaceutical composition has the effect of treating oral inflammation.