14 results about "Hepcidin" patented technology

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The application discloses application of a Hepcidin modulator. Abnormal increase of Hepcidin in a ventral hippocampus region in a senescence process drives imbalance of iron homeostasis of neurons, and further induces an anxiety-like phenotype. Pharmacodynamic verification shows that administration of a Hepcidin inhibitor to cells of the ventral hippocampus can effectively reverse anxiety behavior of a senescence model animal. The pharmaceutical composition described in the application takes the Hepcidin pathway as an intervention target, and an active ingredient thereof is selected from the following: (1) a high-affinity Hepcidin inhibitor NOX-H94; and (2) a recombinant adeno-associated virus vector carrying a Hepcidin-targeted interference sequence, which is used for silencing expression of Hepcidin in neurons of the ventral hippocampus in vivo. The above-mentioned preparation provides a new molecular target and a drug candidate for clinical intervention of senescence-related emotional disorders.

The application relates to a hepcidin chemiluminescence immunoassay kit and a non-diagnostic method for detecting hepcidin, the kit comprising a calibrator, a quality control, an integrated bifunctional antibody reagent, a magnetic particle reagent, a cleaning solution and a chemiluminescence substrate solution; the integrated bifunctional antibody reagent is a buffer containing HEP-specific antibodies covalently connected with fluorescein isothiocyanate and alkaline phosphatase through pH-sensitive connecting bonds; the magnetic particle reagent is a magnetic microsphere suspension coated with a goat anti-fluorescein isothiocyanate antibody; the cleaning solution is a buffer containing a surfactant composition, and the surfactant composition comprises nonionic surfactants, zwitterionic surfactants and high-molecular nonionic surfactants. The hepcidin chemiluminescence immunoassay kit has the effects of ultra-low background noise, ultra-high sensitivity, ultra-wide linear range and simplified operation of end users by means of the integrated bifunctional antibody reagent and the surfactant composition cleaning solution.

This invention provides a method for treating anemia in subjects with myelofibrosis. [Solution] A method is provided for treating anemia in a subject with myelofibrosis, comprising administering an effective amount of a hemoduvelin-induced BMP signaling antagonist, a hepcidin antagonist, to the subject.

Aspects of the application provide siRNAs that target and reduce the expression of hemochromatosis type 2 (HFE2), compositions and methods of using the same to reduce serum hepcidin concentration and treat anemias of inflammation, iron-restricted anemias and / or conditions associated with anemia.

The present invention belongs to the technical field of medicine. Disclosed in the present invention is a new pharmaceutical use of hepcidin, specifically the use of hepcidin in the preparation of a drug for blocking ferroptosis of a collecting tubule in acute kidney injuries and protecting kidney function. It has been demonstrated in the present invention that both renal endogenous and exogenous hepcidin can alleviate tubular injury and improve renal function in ischemia-reperfusion-induced acute kidney injury by inhibiting ferroptosis in intercalated cells of the collecting tubule. Disclosed in the present invention is a drug for alleviating ferroptosis of a collecting tubule and protecting kidney function in ischemic-reperfusion-induced acute kidney injury. The drug is an intraperitoneal injection prepared by dissolving hepcidin at a therapeutic concentration in PBS.

The disclosure provides compositions and methods for the treatment and / or prevention of sickle cell disease.

The present disclosure provides small molecule compounds that are hepcidin mimetics. In addition, the present disclosure also encompasses pharmaceutical compositions that include the small molecule compounds and a pharmaceutically acceptable carrier, excipient or vehicle. The present disclosure also encompasses kits that include the small molecule compounds or the pharmaceutical compositions. The present disclosure also encompasses methods of using the hepcidin mimetic compounds.

Aspects of the application provide hepcidin antagonists and methods of using the same in treating myelofibrosis and / or conditions associated with myelofibrosis.

The invention discloses a fish-derived antibacterial peptide as well as a preparation method and application thereof, the antibacterial peptide is derived from fish-derived Piscid-1 and Hepcidin, and the antibacterial peptide sequentially comprises a signal peptide, an antibacterial peptide, tag protein and other functional elements from an N end to a C end. According to the invention, by optimizing the connection method and sequence of fish-derived Piscid-1, Hepcidin, signal peptide, tag protein and other functional elements in the fish-derived antibacterial peptide, a plurality of engineering bacteria for expressing monomer fish-derived antibacterial peptide and tandem fish-derived antibacterial peptide are obtained, and optimal biological activity and function synergy are realized. The fish-derived antibacterial peptide and the engineering bacteria thereof can be used as feed additives to be applied to fish culture, and can play roles in promoting growth, enhancing immune performance, improving intestinal flora and the like. The method has a wide application prospect.

This application discloses a functional composition, a conditioning agent, and its application in the preparation of a supplement for improving sub-health conditions. The functional composition comprises a first active ingredient and a second active ingredient. The first active ingredient includes red raspberry and carrot, and the second active ingredient includes one or more of artichoke extract and Fusarium oxysporin. The mass ratio of the first active ingredient to the second active ingredient is (1-10000):(1-10000). This application, through the formulation of active ingredients, promotes the body's absorption of iron through mechanisms such as intestinal iron absorption and / or regulation of hepcidin, thereby supplementing iron and significantly improving iron deficiency. Therefore, the functional composition provided by this application can be used to improve symptoms such as anemia caused by functional iron deficiency. Simultaneously, the functional composition also has beneficial effects such as regulating constitution, replenishing qi and blood, and improving sub-health conditions.