651 results about "Chemiluminescence" patented technology

The present invention relates to the technical field of signal processing and fault diagnosis. The method of the present invention comprises: collecting a flame chemiluminescence signal, and performing adaptive filtering noise reduction processing; using an improved proper orthogonal decomposition method and a high-order mode to obtain comprehensive combustion feature information; developing a real-time synchrosqueezing transform algorithm to perform time-frequency analysis on data, and updating energy distribution in real time; taking into account sensor data to perform multi-parameter joint analysis to obtain a combustion state evaluation, using a plurality of high-speed cameras to acquire flame images from different angles, reconstructing three-dimensional flame morphology by means of a computer vision technology, and providing fault detection information; and constructing a fault prediction model to give an early warning of a combustion fault. The present method implements nondestructive and rapid detection of oscillating combustion faults, keeps rich information of flame images, reflects overall flame pulsation characteristics, increases the speed and accuracy of detection, and compensates for the defect of low frequency resolution of conventional time-frequency analysis methods.

This invention discloses a monoclonal antibody against human hepatitis B e antigen and its application, relating to the field of hepatitis B detection technology. In the heavy chain variable region of the monoclonal antibody, the amino acid sequences of the complementarity-determining regions (CDR1-3) are as shown in SEQ ID NO. 1-3 or have at least 95% homology with the sequences shown in SEQ ID NO. 1-3; in the light chain variable region, the amino acid sequences of the complementarity-determining regions (CDR1 and CDR3) are as shown in SEQ ID NO. 4 and 5 or have at least 95% homology with the sequences shown in SEQ ID NO. 4 and 5, and the amino acid sequence of the complementarity-determining region (CDR2) is FAS. The monoclonal antibody provided by this invention can effectively recognize natural hepatitis B e antigen, and the titer of the supernatant from B cell culture can reach 1:10000, making it fully applicable to the detection and research of human hepatitis B e antigen. The chemiluminescent reagent prepared using the monoclonal antibody described in this invention has advantages such as high specificity, strong anti-interference ability, high detection sensitivity, and good stability, with almost no missed detections, and can replace imported reagents.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The embodiment of the invention discloses a refrigerator bacterium detection method and system based on an optical fiber ATP sensor, and the method comprises the steps: mixing a sample in a to-be-detected area of a refrigerator with a reaction reagent to form a to-be-detected solution, injecting the to-be-detected solution into a microfluidic reaction tank of the optical fiber ATP sensor, enabling an ATP component and an enzyme reagent to generate a chemiluminescence reaction, and enabling an optical signal to enter a transmission optical fiber through focusing coupling; the optical signal is transmitted to a photoelectric detection unit through a transmission optical fiber to be converted into an electric signal, and the electric signal contains response dynamic information; carrying out multi-stage processing on the electric signal, and extracting a signal characteristic parameter representing the concentration of the ATP component; and finally, performing matching analysis on the signal characteristic parameters and preset data, determining an ATP component content value, querying corresponding relation data to generate a detection result containing bacteria quantity information, and outputting the detection result to a refrigerator matched display device in a visual form. The problems of long detection time, complicated operation, inconvenience in equipment, difficulty in in-situ real-time monitoring and the like in the existing refrigerator bacterium detection technology can be solved.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The application relates to a microfluidic portable food safety analyzer, a machine core comprises a base, and a top shell, an optical acquisition module, a pressing assembly, a heating module and a centrifugal assembly which are arranged on the base, and the top shell covers the optical acquisition module, the heating module and the centrifugal assembly; the centrifugal assembly is arranged to bear a disc-type chip to be detected, the optical acquisition module is arranged to be close to a first preset position of the disc-type chip, and the heating module is arranged to be close to a second preset position of the disc-type chip. The chemiluminescence detection is realized, qualitative, semi-quantitative or full-quantitative detection can be realized, the detection sensitivity is wider, and the accuracy is higher; no complex optical path design is adopted, and the precision required by positioning is relatively reduced, so that the requirement of positioning precision is met, and a relatively large error of a detection result is avoided; the device is suitable for detecting the disc-type chip, and detection results of multiple items can be obtained at a time, so that the requirement of high throughput is met; the device has the advantages of simple structure, small overall size, light weight and the like, and is convenient to carry and maintain.

The invention discloses a full-automatic chemiluminiscence detection reagent for a porcine reproductive and respiratory syndrome virus N antibody and application of the full-automatic chemiluminiscence detection reagent, and belongs to the technical field of biology. The detection reagent comprises a serum diluent, a first reagent and a second reagent, the first reagent comprises magnetic particles coated with porcine reproductive and respiratory syndrome virus N protein; and the second reagent comprises an acridinium ester labeled anti-pig antibody. The kit can accurately detect the porcine reproductive and respiratory syndrome virus N antibody, is high in sensitivity, good in specificity, strong in interference resistance, high in precision, short in reaction time, simple to operate and automatic, provides material guarantee and technical support for prevention and control of porcine reproductive and respiratory syndrome, and has important significance.

The invention discloses an early diagnosis and early screening kit for joint detection of Parkinson's disease and application of the kit, and relates to the technical field of medical treatment. The kit is used for combined detection of three proteins, namely alpha synuclein (alpha-Syn), tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activated protein zeta (YWHAZ) and myosin light chain 6 (Myl6), immunochromatography, chemiluminescence and other methods and a double-antibody sandwich method are adopted, the diagnosis efficiency of the YWHAZ protein is prominent, the AUC reaches 0.96, and the detection sensitivity is high. The AUC of Myl6 is 0.92, the AUC of alpha-Syn is 0.82, the joint detection AUC is up to 0.98, the kit is newly added with a time-resolved fluorescence immunochromatography technology, low-background and high-sensitivity detection is realized through a lanthanide-labeled antibody, the change of related protein expression level can be detected in the early stage of Parkinson's disease, early screening and diagnosis are realized, and the kit has the advantages of high specificity, joint detection up to 95%, high sensitivity, high sensitivity and the like. The joint detection reaches 96%, the operation is simple and convenient, and the sample requirement is low.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses an SVR combustion instability prediction method and system, and relates to the field of energy power and intelligent control, and the method comprises the steps: obtaining a fault combustion flame chemiluminescence signal, and generating a signal data set comprising a training data set and a test data set; establishing a support vector regression model based on the training data set; evaluating the support vector regression model by using the test data set, and adjusting according to an evaluation result to obtain a prediction model; using the prediction model to predict to-be-predicted sample data; the historical flame chemiluminescence signal data of the gas turbine under different working conditions are adopted as data input and can be used for predicting the working state of the gas turbine after a certain time delay, and the model can perform algorithm processing on the flame chemiluminescence signal measured by the gas turbine and predict the change trend of the future moment. The occurrence time of the unstable combustion can be predicted, and enough time is reserved for the control process of the unstable combustion.

The invention discloses a chemiluminescence heparin binding protein kit and a determination method thereof. The kit comprises reagent components of a reagent R1, a reagent R2 and a reagent R3, as well as a matched calibrator, a matched quality control product, a matched pre-excitation liquid, a matched excitation liquid and a matched cleaning liquid, wherein the reagent R1 contains magnetic particles coated with streptavidin, and the reagent R2 and the reagent R3 are respectively monoclonal antibodies which are labeled by acridinium ester and biotin and are used for recognizing different epitopes of human HBP. The kit shows better sensitivity, wider detection range and high specificity, and can effectively reduce potential endogenous and exogenous interference in clinical samples of potential interference people.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The invention discloses a method for detecting multiple biomarkers based on a nano-enzyme chemiluminescence technology, and the method comprises the following steps: preparing iron oxide nano-particles as a nano-enzyme core, and coating the surfaces of the nano-particles with substrate molecules to form a nano-enzyme-substrate compound; mixing and incubating the nano enzyme-substrate compound and a sample to be detected, adding a hydrogen peroxide solution, transferring to a microplate, and collecting a chemiluminescence signal by using a microplate reader; extracting characteristic parameters including peak value luminous intensity, peak reaching time, half-peak width, rising rate, attenuation rate, integral intensity and / or wavelength ratio in the collected luminous curve, wherein the characteristic parameters comprise the peak value luminous intensity, the peak reaching time, the half-peak width, the rising rate, the attenuation rate and the integral intensity; and constructing and training a multi-layer neural network model so as to predict the concentration of the multiple biomarkers based on the characteristic parameters of the sample data. By means of the scheme, multiple biomarkers can be detected at the same time, and the detection accuracy is improved.

The invention discloses a gold nanocluster electrogenerated chemiluminescence probe which is a multifunctional gold nanocluster obtained by taking a 2-sulfydryl-1, 3, 4-thiadiazole ligand as a reducing agent or a protective agent. Comprising a solid-phase probe with a gold cluster modified on an electrode; or the gold cluster is independent of the electrode and is a liquid-phase probe dissolved in the electrolyte during use. The obtained electrogenerated chemiluminescence gold nanocluster probe can generate an electrogenerated chemiluminescence signal under the condition of no co-reactant, and is relatively low in luminescence potential, high in quantum yield and good in biocompatibility.

The invention provides a preparation method of streptavidin magnetic beads, and relates to the technical field of preparation of polymer composites.The preparation method comprises the steps that S1, PS / SiO2 composite microspheres are prepared; s2, carboxyl magnetic beads are prepared on the basis of the PS / SiO2 composite microspheres prepared in the step S1; s3, activating the carboxyl magnetic beads; s4, carrying out covalent coupling on the carboxyl magnetic beads and streptavidin; step S5, stripping of the physically combined streptavidin; and S6, sealing and storing the streptavidin magnetic beads. The streptavidin magnetic beads prepared through the method are controllable in particle size, superparamagnetic, good in stability and high in activity, compared with a traditional method, a cross-linking agent does not need to be additionally added, the process is effectively simplified, the cost is reduced, the streptavidin magnetic beads have excellent performance in the using process of a chemiluminescence platform, and the process is easy to amplify and beneficial to control.

The invention belongs to the technical field of biological detection, and particularly relates to a composite system for MCP-1 magnetic particle chemiluminescence detection, application and a product. The composite system comprises a coupling buffer solution and a labeling buffer solution, the coupling buffer solution is composed of MES, NaCl, PEG 4000, PVP K30, Tween-20, glycerin and water, and the pH value of the coupling buffer solution is 5.5-6.5; the immunolabeling buffer solution is composed of boric acid, CAPS, NaCl, cane sugar, PEG 6000, Triton X-100 and water, and the pH value of the immunolabeling buffer solution is 9.0-10.0. The sensitivity of the kit containing the composite system can reach 2.31 pg / mL; the linear relation in the concentration range is good; the accuracy is high; the repeatability is high. The method can be used for researching the pathogenesis of the MCP-1 signal channel participating in diseases, screening MCP-1 inhibition drugs and qualitatively and quantitatively detecting the MCP-1.

To provide a method for evaluating the degree of oxidation of an oxidized polymer material.SOLUTION: When a standard piece made of a non-oxidized polymer material is measured by a chemiluminescence method while being heated in an oxygen-containing atmosphere to obtain a first integrated emission amount A, and a specimen made of the oxidized polymer material is measured by the chemiluminescence method while being heated in an oxygen-containing atmosphere to obtain a second integrated emission amount B, the degree of oxidation of the specimen is evaluated on the basis of an emission change rate R=(A-B) / A.SELECTED DRAWING: None

The invention provides multi-channel multi-joint inspection POCT full-automatic chemiluminescence equipment. The equipment comprises a rack; the sampling gun head accommodating mechanism, the test tube accommodating mechanism and the HGB detection mechanism are arranged on the rack in a longitudinal reciprocating motion manner, and a treating fluid container is arranged on the sampling gun head accommodating mechanism; the sampling mechanism is arranged on the rack in a transverse reciprocating motion manner; the reaction bin mechanism can be longitudinally arranged on the rack in a reciprocating manner and comprises a reaction bin body used for loading multiple joint inspection reagent strips, and the reaction bin body is provided with pressing elastic pieces used for pressing the multiple joint inspection reagent strips; the clamping and locking mechanism is located below the reaction bin mechanism, the reaction bin mechanism further comprises a driving device and a synchronous conveying belt connected with the driving device and a reaction bin body, and the clamping and locking mechanism is used for clamping, locking or loosening and unlocking the synchronous conveying belt so as to lock or unlock the reaction bin body. According to the present invention, the device has the immunoassay function, the HGB detection can be achieved, the multi-joint detection reagent strip does not shake or tilt, and the reaction bin body can be locked.

The application discloses a kind of chemiluminescence kits, including box cover, reagent kit body frame and reagent kit body, box cover includes upper cover mechanism and lower cover mechanism, upper cover mechanism and lower cover mechanism one end are connected with each other rotation, upper cover mechanism is integrally formed with upper cover round mouth, lower cover mechanism is integrally formed with lower cover outer round mouth, when upper cover mechanism and lower cover mechanism are closed, upper cover round mouth and lower cover outer round mouth just interference fit, reagent kit body upper mouth is integrally formed with reagent kit upper pipe mouth, reagent kit upper clamping position edge and reagent kit lower clamping position edge from top to bottom, lower cover mechanism inside is provided with lower cover buckle and lower cover inner round mouth, lower cover inner round mouth just with reagent kit upper pipe mouth interference fit, lower cover buckle is better with reagent kit upper clamping position edge clamping fit, reagent kit lower clamping position edge and reagent kit body frame mounting hole edge just fit installation.By reagent kit frame assembly reagent kit, it can be realized by one reagent bottle, also can be formed by multiple reagent bottle components, while reagent kit frame provides positioning edge, facilitate mechanization assembly positioning, reduce assembly process improve assembly and transportation efficiency, by lever structure and the feature of outer round mouth on reagent kit cover and the feature of inner piston on reagent kit mouth carry out interference fit, on the one hand, realize the sealing of bottle mouth circumference side wall, on the other hand, avoid the micro-foreign matter caused by friction into reagent bottle.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats)-based homogeneous chemiluminescence kit as well as a preparation method and a use method thereof, and belongs to the technical field of nucleic acid detection. The kit comprises a Cas enzyme, crRNA, a chemiluminescence reporter molecule, a free radical scavenger and hydrogen peroxide. The chemiluminescent reporter molecule comprises a reporter molecule modified with a chemical group for coupling, one end of the reporter molecule is connected with a peroxidase-like catalytic activity center for reacting with peroxide to generate active free radicals, and the other end of the reporter molecule is connected with a luminescent substrate for reacting with the active free radicals to generate luminescence. According to the kit, a multifunctional microplate reader can be used for collecting chemiluminescence signals for quantifying the concentration of target nucleic acid. The kit is simple in operation steps and short in detection time, does not need light source excitation, and avoids self-luminous interference of a biological sample matrix, so that the kit has more excellent sensitivity and detection dynamic range.

The invention belongs to the technical field of blood detection control, and particularly discloses and provides a full-automatic chemiluminescence analyzer control system for blood sample detection, which is characterized in that regional temperature difference calculation and thermal inertia trend analysis are carried out, and fusion analysis is carried out on a reaction temperature deviation degree and a space coordinate boundary of a thermal inertia imbalance region, so that the thermal inertia imbalance can be accurately detected. And outputting a power adjustment instruction set for different independent heating areas of the reaction disc, carrying out risk assessment based on a preset temperature stability judgment rule, triggering a compensation control strategy when a risk exists, and finally generating a stable temperature field control signal based on the compensation control strategy and the power adjustment instruction set and carrying out corresponding control. The temperature uniformity and stability of the reaction disc are effectively improved, and the precision and repeatability of a chemiluminescence detection result are remarkably improved. And meanwhile, the stability of the temperature adjusting process is also ensured, and the environmental stability required by long-time chemical reaction is maintained.

The invention discloses a method for detecting glyphosate residues in coffee in fluorescence and chemiluminescence dual modes, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that succinic acid serves as a ligand, Ce < 4 + > serves as a central ion, a Suc-Ce complex is synthesized through a microwave method, then the Suc-Ce complex is further subjected to alkali treatment to obtain Suc-Ce-OH nano-enzyme, and the Suc-Ce-OH nano-enzyme is used for detecting glyphosate residues in coffee. The Suc-Ce-OH nano-enzyme has excellent phosphatase-like activity, surface coordinated water molecules can be effectively removed through alkali treatment, more Ce-O active sites are exposed, and the hydrolysis efficiency of the Suc-Ce-OH nano-enzyme on a fluorescent substrate 4-MUP and a chemiluminescent substrate AMPPD is improved by two times; based on the specific inhibition effect of glyphosate on phosphatase-like nano-enzyme activity, a glyphosate fluorescence and chemiluminescence dual-mode detection method is established, the detection limits of the glyphosate fluorescence and chemiluminescence dual-mode detection method are 0.05 mu g / mL and 0.01 mu g / mL respectively, and the glyphosate fluorescence and chemiluminescence dual-mode detection method is used for detecting glyphosate residues in a coffee sample and has the advantages of being high in specificity, easy to operate and sensitive.

The invention discloses a method for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme catalytic chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that 4-aminoantipyrine serves as a precursor, Cu < 2 + > serves as a doping element, copper-doped carbon dots (Cu-CDs) are synthesized through a microwave method, Cu-CDs and ABEI serve as reducing agents to synthesize the Cu-CDs / ABEI (at) Ag nano-enzyme, and the Cu-CDs / ABEI (at) Ag nano-enzyme is used for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme. The Cu-CDs / ABEI (at) Ag nano-enzyme has excellent peroxidase (POD-like) activity, so that H2O2 decomposition is promoted, and a chemiluminescence signal is enhanced; the glyphosate can interact with the Cu-CDs / ABEI (at) Ag nano-enzyme to inhibit the POD-like activity of the nano-enzyme, so that the chemiluminescence intensity generated by oxidation of ABEI is reduced; on the basis, the method is used for detecting glyphosate in a coffee sample, and has the characteristics of high specificity, simplicity in operation and sensitivity.

The invention discloses an electrochemical microscopic imaging analysis method based on a fluorescence signal, which comprises the following steps of: constructing a fluorescence response electrochemical signal instrument by combining electrochemistry with a fluorescence microscope, and observing the redox potential of K3 [Fe (CN) 6] by taking a terpyridyl ruthenium (Ru (bpy) 32 +) / potassium ferricyanide (K3 [Fe (CN) 6]) fluorescence system as a research object. Influences of electrochemical behaviors on fluorescence signals are deeply explored, and the relationship between fluorescence signal changes and electric signal changes is revealed. According to the present invention, the combination of the electrochemical technology and the fluorescence signal is achieved, the disadvantage of the poor spatial-temporal resolution of the electrochemiluminescence is made up, the new dimension capable of being regulated and controlled by the electrical parameter is provided for the fluorescence imaging, and the Faraday current and the non-Faraday current can be completely reflected by the fluorescence signal. Therefore, the method has wide application value in the aspects of exploring the conductivity of the nano material and the like.

The invention belongs to the technical field of biochemistry, and discloses a chemiluminescent probe responding to beta amyloid protein as well as a preparation method and application thereof. The structural formula of the probe is shown in the specification. According to the probe designed by the invention, the near-infrared chemiluminescence capability (gt; 650nm) is realized through an extended conjugated structure; meanwhile, high selectivity on A beta is realized by introducing a heteroatom conjugate pi bridge.

The invention relates to the field of antibodies, in particular to an anti-Der p 1 antibody and application thereof. The invention provides an anti-Der p 1 antibody, the anti-Der p 1 antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises an amino acid sequence of a complementarity determining region, and the amino acid sequence of the complementarity determining region comprises CDR1 as shown in SEQ ID No.1, CDR2 as shown in SEQ ID No.2 and CDR3 as shown in SEQ ID No.3; or the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.8. The anti-Der p1 antibody provided by the invention is a monoclonal antibody with high affinity and high sensitivity, after the monoclonal antibody is subjected to humanized IgE transformation, the monoclonal antibody is applied to a Dermatophagoides pteronyssinus allergen component Derp1 specificity IgE magnetic particle chemiluminescence quantitative detection reagent, and a standard curve independently calibrated by the Dermatophagoides pteronyssinus allergen component Derp1 can be obtained; real, accurate and repeatable absolute quantification is realized, the Dermatophagoides pteronyssinus allergen component Derp 1 specificity IgE with the concentration of 0.1 IU / mL can be detected, and the method has clinical diagnosis significance in allergic reaction identification.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

Methods of detecting analytes in a sample are described herein using chemiluminescent labels and branched DNA. Solid supports, reagents, and compounds for use in these methods are also described. Typically, the methods involve specific assay formats which provide the requisite high resolution for detecting low concentrations of analytes such as exosome protein biomarkers in samples.

A method for electrochemiluminescence detection of hydroquinone based on aggregation-induced emission Cd-MOF is provided, including: step 1, preparation of Cd-MOF material; step 2, mixing Cd-MOF material with co-reactant; and step 3, establishment and application of hydroquinone sensor. The organic ligand TPPE with aggregation-induced emission effect and CdCl2 are used to prepare self-luminous Cd-based metal-organic framework Cd-MOF with rigid and directional structure through coordination.