2873 results about "Chemiluminescence" patented technology

Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electrochemiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.

The disclosure describes how to use luminescence detection mechanism, move microfluid, and control multiple-step biochemical reactions in closed confined microfluidic biochip platform. More particularly, a self-contained disposable biochip with patterned microchannels and compartments having storage means for storing a plurality of samples, reagents, and luminescent substrates. At least one external microactuator in the biochip system produces positive pressure and automates multiple-step reactions in microfluidic platforms for clinical chemistry, cell biology, immunoassay and nucleic acid analysis. The method comprises the steps of transferring sequentially at least one of samples, reagents, and then luminescent substrate from compartments through microchannels to reaction sites. The luminescent substrates react with probes to form a probe complex resulting into luminescence, which is detected by an optical detector.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, charcterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have calorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

A method and apparatus for measuring electrochemiluminescence from a sample composition are described wherein magnetically responsive electrochemiluminescent active species are captured on the electrode with the aid of a capture magnet having a configuration such that the magnetic flux lines (or the magnetic field gradient) of at least one magnetic field source therein are compressed and/or dispersed. This capture magnet improves the distribution of the magnetically responsive electrochemiluminescent active species on the electrode surface and reduces interference with the photomultiplier tube, thereby enhancing the ECL signal and improving sensitivity. The improved capture and distribution also allows for shorter assay times.

Methods and apparatus for performing a binding assay for an analyte of interest present in a sample are described. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

Described herein is an apparatus comprising an electrochemical cell that employs a capacitive counter electrode and a faradaic working electrode. The capacitive counter electrode reduces the amount of redox products generated at the counter electrode while enabling the working electrode to generate redox products. The electrochemical cell is useful for controlling the redox products generated and/or the timing of the redox product generation. The electrochemical cell is useful in assay methods, including those using electrochemiluminescence. The electrochemical cell can be combined with additional hardware to form instrumentation for assay methods.

The invention discloses a portable full-automatic chemiluminescence immune assay system and an assay method of the portable full-automatic chemiluminescence immune assay system. The system comprises a sample feeding part, a liquid path part, a reaction control part, an optical detection part and a computer control center, wherein the sample feeding part comprises a double sample feeding arm device, a sample reagent storing cabin and a reaction cup pushing device; the reaction control part comprises an incubation device, a magnetic separation washing device and a transposition device; the optical detection part comprises a chemiluminescence measuring device; the sample feeding part, the liquid path part, the reaction control part and the optical detection part are electrically connected with the computer control center. The system disclosed by the invention is high in automation degree, reasonable in structural design, small in volume and low in manufacturing cost; hardware, software and a liquid path system are all integrated to form a whole body, so that the system is convenient to move and is applicable to chemiluminescence immune assays of hospitals, community health service centers and the like; the full-automatic assay work can be realized; the system effectively ensures that an assay process and an assay result are accurate and personal errors are reduced.

A sports projectile illuminated with a chemiluminescent light stick capable of producing a stroboscopic effect. The body is made of a soft, pliable material such as NERF® and the light stick(s) is carried through bores in the body. There are no ancillary components, only the light stick(s) and the soft, pliable body. Orifices or various other shapes are cut into the body of the sports projections allowing for the maximum light transmission.

The invention discloses a full-automatic chemoluminescence immunoassay analyzer. The analyzer is mainly composed of a working main body and a control computer connected with the working main body; a storage cabin and a control box are arranged under the working main body. The analyzer can be used for implementing the full-automatic operation of the immunoreaction process comprising reaction tube loading, sample automatic injection, reagent automatic injection, reaction solution incubation reaction, reaction solution automatic cleaning, reaction result detection and analysis; the full-automatic operation reduces the influence of human factors on the experiment, thereby improving the sensitivity. Each component of the invention is ingenious in design, the mutual cooperation of components isconvenient for the system integration so as to realize the automation; and based on the full-automatic chemoluminescence immunoassay analyzer, more devices with automatic function can be developed.

The invention provides a method for extracting carbon quantum dots from activated carbon, which comprises the following steps of: adding dry activated carbon powder to salpeter solution and stirring for backflow; performing reduced pressure distillation for evaporating suspension obtained by backflow to dryness; dispersing obtained black solids in water, and neutralizing obtained solution with sodium hydroxide; and finally, centrifugating neutralized black suspension for removing precipitation, separating supernatant fluid by using an ultrafiltration centrifugal tube or an ultrafiltration membrane, collecting filtrate, and drying the filtrate to obtain carbon quantum dots. The method uses cheap and available activated carbon as carbon sources, and can obtain a large number of carbon quantum dots by simple chemical oxidation process and simple subsequent processes of evaporation, saturation, centrifugation and ultrafiltration. The carbon quantum dots are graphite structure nanocrystals with the grain diameter of 3 to 5 nm, the surfaces of which have a large amount of hydroxide radicals. The carbon quantum dots have good fluorescence and electrochemiluminescence.

The invention relates to a molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residues, belonging to the technical field of the detection of the pesticide residues. Theinvention also relates to a method for detecting the trace amount pesticide residues contained in agricultural product samples by using the molecular imprinting chemiluminescence sensor, which comprises the following steps: (1) selecting a functional monomer; (2) uniformly mixing a template molecule, the functional monomer, a cross linker, a pore-forming agent, an initiating agent and a silicon source to prepare an MIPs collosol according to a mole ratio of 0.1-1 to 1-10 to 5-25 to 20-60 to 0.05-0.15 to 20-50; (3) preparing a quantum dot material solution; and (4) modifying the MIPs collosoland quantum dot materials to the surface of a millipore plate to manufacture the chemiluminescence sensor by a layer-layer self-assembly surface modification technique. The invention has the advantages of high specificity and sensitivity, fast detection, low cost, good repeatability and convenient field detection on the detection of the pesticide residues.

A method for improving the sensitivity of an immunoassay by a factor of at least 10 to about 25, and perhaps greater. Furthermore, in addition to increasing the sensitivity of the assay, the method of this invention also improves assay specificity. In order to match or improve upon sensitivity of 0.2 pg/mL for an analyte, this invention provides an immunoassay involving amplification of a signal, e.g., a chemiluminescent signal, a specific binding member, e.g., a monoclonal antibody, and microparticle separation, e.g., magnetic microparticle separation, from large volumes of sample (e.g., from about 0.2 to about 3 mL). Such an immunoassay can be carried out with an automated immunoassay analyzers, e.g., an automated immunoassay analyzer in the ARCHITECT® family of analyzers.