1139 results about "Chemiluminescence" patented technology

The invention discloses an anti-human hepatitis B e antigen monoclonal antibody and application thereof, and relates to the technical field of hepatitis B detection. In a heavy chain variable region of the monoclonal antibody, an amino acid sequence of a complementarity determining region CDR1-3 is as shown in SEQ ID NO.1-3 or has at least 95% of homology with the sequence as shown in SEQ ID NO.1-3; in the light chain variable region, amino acid sequences of complementary determining regions CDR1 and CDR3 are shown as SEQ ID NO.4 and SEQ ID NO.5 or have at least 95% of homology with the sequences shown as SEQ ID NO.4 and SEQ ID NO.5, and an amino acid sequence of a complementary determining region CDR2 is FAS. The monoclonal antibody provided by the invention can well recognize a natural hepatitis B e antigen, and the titer of a supernatant cultured by a B cell can reach 1: 10000, so that the monoclonal antibody can be completely applied to detection research of the human hepatitis B e antigen. The chemiluminescence reagent prepared by using the monoclonal antibody has the advantages of high specificity, strong anti-interference capability, high detection sensitivity, good stability and the like, almost has no leak detection, and can replace an imported reagent.

The invention belongs to the technical field of immunodetection, and discloses a T3 sandwich method rabbit monoclonal antibody mAb3 and application thereof. The T3 sandwich method monoclonal antibody mAb3 provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1-3, the LCDR1 comprises an amino acid sequence NIYNS, the LCDR2 comprises an amino acid sequence YAS, and the LCDR3 comprises an amino acid sequence QSHYGFSSSYGAA; the heavy chain variable region comprises HCDR1-3, the HCDR1 comprises an amino acid sequence GFDVSYN, the HCDR2 comprises an amino acid sequence IDTGANA, and the HCDR3 comprises an amino acid sequence ARVFYDNSFDP. The monoclonal antibody can be combined with a T3 compound in a high-specificity mode, is suitable for double-antibody sandwich immunodetection and is applied to an immunodetection kit prepared through a double-antibody sandwich ELISA or a chemiluminescence method, a T3 standard antigen is detected through a double-antibody sandwich chemiluminescence platform, the detection sensitivity is lower than 0.3 ng / ml, clinical samples are detected through a magnetochemiluminescence method, and the detection sensitivity is lower than 0.3 ng / ml. In the sample range of 1-80 pmol / L, the correlation with clinical comparison is good.

The invention relates to a light-activated chemiluminescence detection kit for phosphorylated Tau protein p-tau 217 as well as a use method and application of the light-activated chemiluminescence detection kit. The kit comprises a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1 comprises a luminous microsphere and a first antibody which is coated on the luminous microsphere, is provided with a first tag molecule and can be specifically combined with p-tau 217; the R2 reagent comprises a second antibody with a second tag molecule; the R3 reagent comprises a first pairing molecule capable of being specifically combined with the first tag molecule; the first antibody and the second antibody can be specifically combined with different epitopes of p-tau 217, and one first pairing molecule can be combined with at least two first tag molecules. According to the technical scheme, the capacity of the light-emitting microspheres for receiving active oxygen generated by the light-sensitive microspheres can be improved, the detection sensitivity is improved, and the light-activated chemiluminescence detection requirement of p-tau 217 in plasma is met.

The present invention relates to the technical field of signal processing and fault diagnosis. The method of the present invention comprises: collecting a flame chemiluminescence signal, and performing adaptive filtering noise reduction processing; using an improved proper orthogonal decomposition method and a high-order mode to obtain comprehensive combustion feature information; developing a real-time synchrosqueezing transform algorithm to perform time-frequency analysis on data, and updating energy distribution in real time; taking into account sensor data to perform multi-parameter joint analysis to obtain a combustion state evaluation, using a plurality of high-speed cameras to acquire flame images from different angles, reconstructing three-dimensional flame morphology by means of a computer vision technology, and providing fault detection information; and constructing a fault prediction model to give an early warning of a combustion fault. The present method implements nondestructive and rapid detection of oscillating combustion faults, keeps rich information of flame images, reflects overall flame pulsation characteristics, increases the speed and accuracy of detection, and compensates for the defect of low frequency resolution of conventional time-frequency analysis methods.

Hydrogen-bonded framework nanoparticles comprising a chemiluminescent compound (e.g., L-012) are provided and can be used, e.g., for sono-optogenetic treatment of neurodegenerative diseases such as Parkinson's disease. Related in vivo and therapeutic methods are also provided.

A method for producing an electrochemiluminescence nanoprobe according to an embodiment includes: a hot exciton nanoparticle synthesis step of polymerizing a hot exciton organic luminescent molecule and a copolymer molecule to synthesize hot exciton nanoparticles; and a hot exciton nanoparticle modification step of modifying the obtained hot exciton nanoparticles with an oligonucleotide chain modified with a quencher molecule to obtain modified hot exciton nanoparticles.

The present application discloses a detection kit of KL-6 protein on exosomes for auxiliary diagnosis of interstitial lung diseases. A detection method includes: after pretreating the exosome sample with a sample treatment solution, exosomes containing KL-6 protein in the sample react with a coated magnetic bead working solution and a luminescent marker-labeled antibody working solution to form an immunocomplex, and a luminous intensity is detected by using a chemiluminescence method and a concentration value is calculated. The application provides, for the first time, a method for diagnosing interstitial pneumonia using a combination of KL-6 and CA125 proteins on exosomes extracted from blood, or using KL-6 alone.

The invention belongs to the technical field of immunodetection, and discloses an anti-T3 antibody mAb32 or an antigen binding fragment, the anti-T3 antibody mAb32 or the antigen binding fragment comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1, LCDR2 and LCDR3, the amino acid sequence of the LCDR1 is shown as SEQ ID NO.1, the amino acid sequence of the LCDR2 is IAS, and the amino acid sequence of the LCDR3 is shown as SEQ ID NO.3; the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the HCDR1, the HCDR2 and the HCDR3 are respectively shown as SEQ ID NO. 4-6. When the anti-T3 antibody mAb32 is used for detecting a T3 standard antigen, the detection sensitivity is lower than 0.2 ng / ml, and when a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good within the sample range of 1-80 pmol / L.

This invention discloses a monoclonal antibody against human hepatitis B e antigen and its application, relating to the field of hepatitis B detection technology. In the heavy chain variable region of the monoclonal antibody, the amino acid sequences of the complementarity-determining regions (CDR1-3) are as shown in SEQ ID NO. 1-3 or have at least 95% homology with the sequences shown in SEQ ID NO. 1-3; in the light chain variable region, the amino acid sequences of the complementarity-determining regions (CDR1 and CDR3) are as shown in SEQ ID NO. 4 and 5 or have at least 95% homology with the sequences shown in SEQ ID NO. 4 and 5, and the amino acid sequence of the complementarity-determining region (CDR2) is FAS. The monoclonal antibody provided by this invention can effectively recognize natural hepatitis B e antigen, and the titer of the supernatant from B cell culture can reach 1:10000, making it fully applicable to the detection and research of human hepatitis B e antigen. The chemiluminescent reagent prepared using the monoclonal antibody described in this invention has advantages such as high specificity, strong anti-interference ability, high detection sensitivity, and good stability, with almost no missed detections, and can replace imported reagents.

The invention relates to the technical field of immunodetection and immunodiagnosis, in particular to a chemiluminescence substrate solution, a chemiluminescence detection kit and a detection method. The chemiluminescent substrate solution comprises a substrate solution 1, or comprises the substrate solution 1 and a substrate solution 2, the substrate solution 1 comprises sodium hydroxide, a surfactant and a blue light absorbent for absorbing blue light with the wavelength of 400-500 nm; the substrate solution 2 comprises inorganic acid and hydrogen peroxide. According to the application, the blue light absorbent is added into the chemiluminescent substrate liquid, so that blue light emitted by acridinium ester can be absorbed, a luminescent signal is reduced, and particularly, the luminescent value of a background caused by non-specific adsorption is reduced, so that the signal-to-noise ratio is increased.

The invention discloses a magnetic particle chemiluminiscence detection kit for human non-phosphorylated Tau217 protein and a preparation method of the magnetic particle chemiluminiscence detection kit. The kit comprises a micro magnetic particle coupled with a human non-phosphorylated Tau217 protein capture antibody, an acridinium ester labeled human non-phosphorylated Tau217 protein detection antibody, a human non-phosphorylated Tau217 protein calibration product and a quality control product. The direct chemiluminescence method established by the invention has the advantages of high sensitivity, strong specificity, accuracy, rapidness and short detection time, the detection result has higher accuracy and repeatability, and the kit can be used for various luminescence detection instruments.

The invention belongs to the technical field of immunodetection, and discloses an anti-T3 antibody mAb68 or an antigen binding fragment, the anti-T3 antibody mAb68 or the antigen binding fragment comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1, LCDR2 and LCDR3, the amino acid sequence of the LCDR1 is shown as SEQ ID NO.1, the amino acid sequence of the LCDR2 is TAS, and the amino acid sequence of the LCDR3 is shown as SEQ ID NO.3; the heavy variable region comprises HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the HCDR1, the HCDR2 and the HCDR3 are respectively shown as SEQ ID NO. 4-6. When the anti-T3 antibody mAb68 is used for detecting a T3 standard antigen, the detection sensitivity is lower than 0.2 ng / ml, a clinical sample is detected through a magnetochemiluminescence method, and the correlation with R clinical comparison is good within the sample range of 1-80 pmol / L.

The invention provides an anti-beta2-glycoprotein I antibody detection reagent and a chemiluminescence detection kit, and the anti-beta2-glycoprotein I antibody detection reagent comprises a biotin-labeled beta2-glycoprotein I antigen prepared by reacting biotin connected with an LPETG label with the beta2-glycoprotein I antigen under the catalytic action of Sortase A enzyme. The N terminal of the beta2-glycoprotein I antigen is labeled with the biotin with a specific label in a fixed point manner, all amino acid sequence regions (structural domains I-V) of the beta2-glycoprotein I antigen are not influenced, and meanwhile, the purity of a connection product is high, the difference between batches is small, and the method is suitable for large-scale production. The positive detection rate and the detection result accuracy of the anti-beta2-glycoprotein I antibody magnetic particle chemiluminescence detection kit can be improved, and the difference between batches can be reduced.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention provides a dilution buffer solution for chemiluminescence detection of a blood sample. The dilution buffer solution contains 0.05 to 1 weight percent of plasmin and 0.005 to 0.02 weight percent of a de-foaming agent. The dilution buffer solution provided by the invention can reduce the jump value phenomenon in chemiluminescence detection of a blood sample, especially a repeatedly frozen and thawed blood sample, so that a correct and reliable detection result is provided for clinic, and the clinical detection time can be saved.

The embodiment of the invention discloses a refrigerator bacterium detection method and system based on an optical fiber ATP sensor, and the method comprises the steps: mixing a sample in a to-be-detected area of a refrigerator with a reaction reagent to form a to-be-detected solution, injecting the to-be-detected solution into a microfluidic reaction tank of the optical fiber ATP sensor, enabling an ATP component and an enzyme reagent to generate a chemiluminescence reaction, and enabling an optical signal to enter a transmission optical fiber through focusing coupling; the optical signal is transmitted to a photoelectric detection unit through a transmission optical fiber to be converted into an electric signal, and the electric signal contains response dynamic information; carrying out multi-stage processing on the electric signal, and extracting a signal characteristic parameter representing the concentration of the ATP component; and finally, performing matching analysis on the signal characteristic parameters and preset data, determining an ATP component content value, querying corresponding relation data to generate a detection result containing bacteria quantity information, and outputting the detection result to a refrigerator matched display device in a visual form. The problems of long detection time, complicated operation, inconvenience in equipment, difficulty in in-situ real-time monitoring and the like in the existing refrigerator bacterium detection technology can be solved.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention relates to the technical field of signal processing and fault diagnosis, and the method comprises the steps: collecting a flame chemiluminescence signal, and carrying out the adaptive filtering noise reduction processing; comprehensive combustion characteristic information is obtained by using an improved intrinsic orthogonal decomposition method and a high-order mode; developing a real-time synchronous compression transformation algorithm to perform time-frequency analysis on the data, and updating energy distribution in real time; sensor data are combined, multi-parameter conjoint analysis is conducted, combustion state evaluation is obtained, flame images are obtained from different angles through a plurality of high-speed cameras, the three-dimensional flame form is reconstructed through the computer vision technology, and fault detection information is provided; and a fault prediction model is constructed, and early warning of combustion faults is realized. The method realizes lossless and rapid detection of oscillation combustion faults, retains rich information of flame images, reflects the overall pulsation characteristics of flames, improves the detection speed and accuracy, and makes up for the disadvantage of low frequency resolution of a traditional time-frequency analysis method.

The application relates to a microfluidic portable food safety analyzer, a machine core comprises a base, and a top shell, an optical acquisition module, a pressing assembly, a heating module and a centrifugal assembly which are arranged on the base, and the top shell covers the optical acquisition module, the heating module and the centrifugal assembly; the centrifugal assembly is arranged to bear a disc-type chip to be detected, the optical acquisition module is arranged to be close to a first preset position of the disc-type chip, and the heating module is arranged to be close to a second preset position of the disc-type chip. The chemiluminescence detection is realized, qualitative, semi-quantitative or full-quantitative detection can be realized, the detection sensitivity is wider, and the accuracy is higher; no complex optical path design is adopted, and the precision required by positioning is relatively reduced, so that the requirement of positioning precision is met, and a relatively large error of a detection result is avoided; the device is suitable for detecting the disc-type chip, and detection results of multiple items can be obtained at a time, so that the requirement of high throughput is met; the device has the advantages of simple structure, small overall size, light weight and the like, and is convenient to carry and maintain.

The invention discloses a full-automatic chemiluminiscence detection reagent for a porcine reproductive and respiratory syndrome virus N antibody and application of the full-automatic chemiluminiscence detection reagent, and belongs to the technical field of biology. The detection reagent comprises a serum diluent, a first reagent and a second reagent, the first reagent comprises magnetic particles coated with porcine reproductive and respiratory syndrome virus N protein; and the second reagent comprises an acridinium ester labeled anti-pig antibody. The kit can accurately detect the porcine reproductive and respiratory syndrome virus N antibody, is high in sensitivity, good in specificity, strong in interference resistance, high in precision, short in reaction time, simple to operate and automatic, provides material guarantee and technical support for prevention and control of porcine reproductive and respiratory syndrome, and has important significance.

The invention relates to the technical field of chemiluminescence immunoassay, in particular to a simple and efficient vitamin B12 detection kit and a detection method.The simple and efficient vitamin B12 detection kit comprises a pretreatment agent, a magnetic particle system and a marker system, an extracted serum sample is mixed with the protein releasing agent and the complexing agent in the pretreatment agent, and the vitamin B12 in the sample is released from the binding protein; the complexing agent is a complex with cyanide ions, the cyanide ions separated from the complex in an aqueous solution are combined with the released vitamin B12 to obtain stable cyanocobalamin for subsequent detection of the vitamin B12, and the vitamin 12 in a to-be-detected sample is converted into a stable state for detection by the method, so that the detection stability is improved.

The invention discloses a construction method and application of an ultra-sensitive self-enhanced electrochemical luminescence biosensor, and belongs to the technical fields of biochemical analysis, nano materials and life science. According to the preparation method disclosed by the invention, Cu < 2 + > is introduced into luminous ZrEu-MOF, and a co-reactant K2S2O8 is catalyzed by the Cu < 2 + > to generate more active free radicals, so that ZrEuCu-MOF with a self-enhancement function is prepared; resorcinol-formaldehyde resin with an egg yolk structure is further synthesized as a quenching probe, and effective quenching of a light-emitting signal is realized based on electrochemical luminescence (ECL) resonance energy transfer between the resorcinol-formaldehyde resin and ZrEuCu-MOF, so that the detection sensitivity is improved. The constructed ECL biosensor realizes efficient and sensitive detection of neuron-specific enolase, and has the advantages of low detection limit, wide detection range, good stability and industrial application prospect.

The invention discloses an early diagnosis and early screening kit for joint detection of Parkinson's disease and application of the kit, and relates to the technical field of medical treatment. The kit is used for combined detection of three proteins, namely alpha synuclein (alpha-Syn), tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activated protein zeta (YWHAZ) and myosin light chain 6 (Myl6), immunochromatography, chemiluminescence and other methods and a double-antibody sandwich method are adopted, the diagnosis efficiency of the YWHAZ protein is prominent, the AUC reaches 0.96, and the detection sensitivity is high. The AUC of Myl6 is 0.92, the AUC of alpha-Syn is 0.82, the joint detection AUC is up to 0.98, the kit is newly added with a time-resolved fluorescence immunochromatography technology, low-background and high-sensitivity detection is realized through a lanthanide-labeled antibody, the change of related protein expression level can be detected in the early stage of Parkinson's disease, early screening and diagnosis are realized, and the kit has the advantages of high specificity, joint detection up to 95%, high sensitivity, high sensitivity and the like. The joint detection reaches 96%, the operation is simple and convenient, and the sample requirement is low.

The invention discloses a chemiluminescent probe of a targeted A beta oligomer as well as a preparation method and application of the chemiluminescent probe. The structural general formula of the chemiluminescent probe of the targeted A beta oligomer is shown as I in the specification. According to the present invention, the chemiluminescence probe capable of specifically targeting the A beta oligomer is successfully constructed for the first time so as to achieve the early diagnosis and treatment of AD; an additional light source is not needed, and background signals caused by exciting light can be effectively reduced, so that the detection sensitivity is higher. The chemiluminescent probe targeting the A beta oligomer can promote selective oxygenation of beta amyloid protein through chemical excitation and neutralize toxicity of the A beta oligomer, has certain treatment potential and is expected to be developed into an effective method for treating amyloidosis. # imgabs0 #

Methods, kits and compositions of detecting analytes, typically analytes relevant to neurodegenerative diseases such as neurofilament light chain, in a sample are described herein using chemiluminescent labels. Solid supports, reagents, and compounds for use in these methods are also described. Typically, the methods involve specific assay formats which provide the requisite high resolution for detecting low concentrations of analytes in samples and may be used in positions of the healthcare ecosystem close to the patient. These methods, systems, and apparatuses may afford early detection and prognosis of a wide variety of neurodegenerative diseases such as Alzheimer's disease and multiple sclerosis.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses an SVR combustion instability prediction method and system, and relates to the field of energy power and intelligent control, and the method comprises the steps: obtaining a fault combustion flame chemiluminescence signal, and generating a signal data set comprising a training data set and a test data set; establishing a support vector regression model based on the training data set; evaluating the support vector regression model by using the test data set, and adjusting according to an evaluation result to obtain a prediction model; using the prediction model to predict to-be-predicted sample data; the historical flame chemiluminescence signal data of the gas turbine under different working conditions are adopted as data input and can be used for predicting the working state of the gas turbine after a certain time delay, and the model can perform algorithm processing on the flame chemiluminescence signal measured by the gas turbine and predict the change trend of the future moment. The occurrence time of the unstable combustion can be predicted, and enough time is reserved for the control process of the unstable combustion.

The invention discloses a chemiluminescence heparin binding protein kit and a determination method thereof. The kit comprises reagent components of a reagent R1, a reagent R2 and a reagent R3, as well as a matched calibrator, a matched quality control product, a matched pre-excitation liquid, a matched excitation liquid and a matched cleaning liquid, wherein the reagent R1 contains magnetic particles coated with streptavidin, and the reagent R2 and the reagent R3 are respectively monoclonal antibodies which are labeled by acridinium ester and biotin and are used for recognizing different epitopes of human HBP. The kit shows better sensitivity, wider detection range and high specificity, and can effectively reduce potential endogenous and exogenous interference in clinical samples of potential interference people.

The invention relates to a specific chemiluminescent probe for determining CYP3A in the technical field of medicines, which is structurally characterized by being shown in the following formula: # imgabs0 #. The probe structurally comprises a high-specificity reaction response group of CYP3A, a chemiluminescent group, a self-hyperchromic group and a self-leaving linking group connected with the high-specificity reaction response group, the chemiluminescent group and the self-hyperchromic group, the chemiluminescent probe does not need to be excited by an external light source, is free of phototoxicity, has extremely high signal-to-noise ratio and sensitivity, can be used for cell and living body imaging, has the advantages of being free of interference of biological matrixes, different in detection mode and the like compared with a traditional fluorescent probe, guarantees the accuracy of experiments, has the characteristics of detection convenience and high-throughput detection, and is suitable for large-scale popularization and application. The potential application value is realized in new drug research and development and individualized drug treatment.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.