436 results about "Chemiluminescence" patented technology

The present invention relates to the technical field of signal processing and fault diagnosis. The method of the present invention comprises: collecting a flame chemiluminescence signal, and performing adaptive filtering noise reduction processing; using an improved proper orthogonal decomposition method and a high-order mode to obtain comprehensive combustion feature information; developing a real-time synchrosqueezing transform algorithm to perform time-frequency analysis on data, and updating energy distribution in real time; taking into account sensor data to perform multi-parameter joint analysis to obtain a combustion state evaluation, using a plurality of high-speed cameras to acquire flame images from different angles, reconstructing three-dimensional flame morphology by means of a computer vision technology, and providing fault detection information; and constructing a fault prediction model to give an early warning of a combustion fault. The present method implements nondestructive and rapid detection of oscillating combustion faults, keeps rich information of flame images, reflects overall flame pulsation characteristics, increases the speed and accuracy of detection, and compensates for the defect of low frequency resolution of conventional time-frequency analysis methods.

This invention discloses a monoclonal antibody against human hepatitis B e antigen and its application, relating to the field of hepatitis B detection technology. In the heavy chain variable region of the monoclonal antibody, the amino acid sequences of the complementarity-determining regions (CDR1-3) are as shown in SEQ ID NO. 1-3 or have at least 95% homology with the sequences shown in SEQ ID NO. 1-3; in the light chain variable region, the amino acid sequences of the complementarity-determining regions (CDR1 and CDR3) are as shown in SEQ ID NO. 4 and 5 or have at least 95% homology with the sequences shown in SEQ ID NO. 4 and 5, and the amino acid sequence of the complementarity-determining region (CDR2) is FAS. The monoclonal antibody provided by this invention can effectively recognize natural hepatitis B e antigen, and the titer of the supernatant from B cell culture can reach 1:10000, making it fully applicable to the detection and research of human hepatitis B e antigen. The chemiluminescent reagent prepared using the monoclonal antibody described in this invention has advantages such as high specificity, strong anti-interference ability, high detection sensitivity, and good stability, with almost no missed detections, and can replace imported reagents.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The embodiment of the invention discloses a refrigerator bacterium detection method and system based on an optical fiber ATP sensor, and the method comprises the steps: mixing a sample in a to-be-detected area of a refrigerator with a reaction reagent to form a to-be-detected solution, injecting the to-be-detected solution into a microfluidic reaction tank of the optical fiber ATP sensor, enabling an ATP component and an enzyme reagent to generate a chemiluminescence reaction, and enabling an optical signal to enter a transmission optical fiber through focusing coupling; the optical signal is transmitted to a photoelectric detection unit through a transmission optical fiber to be converted into an electric signal, and the electric signal contains response dynamic information; carrying out multi-stage processing on the electric signal, and extracting a signal characteristic parameter representing the concentration of the ATP component; and finally, performing matching analysis on the signal characteristic parameters and preset data, determining an ATP component content value, querying corresponding relation data to generate a detection result containing bacteria quantity information, and outputting the detection result to a refrigerator matched display device in a visual form. The problems of long detection time, complicated operation, inconvenience in equipment, difficulty in in-situ real-time monitoring and the like in the existing refrigerator bacterium detection technology can be solved.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention discloses a full-automatic chemiluminiscence detection reagent for a porcine reproductive and respiratory syndrome virus N antibody and application of the full-automatic chemiluminiscence detection reagent, and belongs to the technical field of biology. The detection reagent comprises a serum diluent, a first reagent and a second reagent, the first reagent comprises magnetic particles coated with porcine reproductive and respiratory syndrome virus N protein; and the second reagent comprises an acridinium ester labeled anti-pig antibody. The kit can accurately detect the porcine reproductive and respiratory syndrome virus N antibody, is high in sensitivity, good in specificity, strong in interference resistance, high in precision, short in reaction time, simple to operate and automatic, provides material guarantee and technical support for prevention and control of porcine reproductive and respiratory syndrome, and has important significance.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The application discloses a kind of chemiluminescence kits, including box cover, reagent kit body frame and reagent kit body, box cover includes upper cover mechanism and lower cover mechanism, upper cover mechanism and lower cover mechanism one end are connected with each other rotation, upper cover mechanism is integrally formed with upper cover round mouth, lower cover mechanism is integrally formed with lower cover outer round mouth, when upper cover mechanism and lower cover mechanism are closed, upper cover round mouth and lower cover outer round mouth just interference fit, reagent kit body upper mouth is integrally formed with reagent kit upper pipe mouth, reagent kit upper clamping position edge and reagent kit lower clamping position edge from top to bottom, lower cover mechanism inside is provided with lower cover buckle and lower cover inner round mouth, lower cover inner round mouth just with reagent kit upper pipe mouth interference fit, lower cover buckle is better with reagent kit upper clamping position edge clamping fit, reagent kit lower clamping position edge and reagent kit body frame mounting hole edge just fit installation.By reagent kit frame assembly reagent kit, it can be realized by one reagent bottle, also can be formed by multiple reagent bottle components, while reagent kit frame provides positioning edge, facilitate mechanization assembly positioning, reduce assembly process improve assembly and transportation efficiency, by lever structure and the feature of outer round mouth on reagent kit cover and the feature of inner piston on reagent kit mouth carry out interference fit, on the one hand, realize the sealing of bottle mouth circumference side wall, on the other hand, avoid the micro-foreign matter caused by friction into reagent bottle.

The invention belongs to the technical field of blood detection control, and particularly discloses and provides a full-automatic chemiluminescence analyzer control system for blood sample detection, which is characterized in that regional temperature difference calculation and thermal inertia trend analysis are carried out, and fusion analysis is carried out on a reaction temperature deviation degree and a space coordinate boundary of a thermal inertia imbalance region, so that the thermal inertia imbalance can be accurately detected. And outputting a power adjustment instruction set for different independent heating areas of the reaction disc, carrying out risk assessment based on a preset temperature stability judgment rule, triggering a compensation control strategy when a risk exists, and finally generating a stable temperature field control signal based on the compensation control strategy and the power adjustment instruction set and carrying out corresponding control. The temperature uniformity and stability of the reaction disc are effectively improved, and the precision and repeatability of a chemiluminescence detection result are remarkably improved. And meanwhile, the stability of the temperature adjusting process is also ensured, and the environmental stability required by long-time chemical reaction is maintained.

The invention discloses a method for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme catalytic chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that 4-aminoantipyrine serves as a precursor, Cu < 2 + > serves as a doping element, copper-doped carbon dots (Cu-CDs) are synthesized through a microwave method, Cu-CDs and ABEI serve as reducing agents to synthesize the Cu-CDs / ABEI (at) Ag nano-enzyme, and the Cu-CDs / ABEI (at) Ag nano-enzyme is used for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme. The Cu-CDs / ABEI (at) Ag nano-enzyme has excellent peroxidase (POD-like) activity, so that H2O2 decomposition is promoted, and a chemiluminescence signal is enhanced; the glyphosate can interact with the Cu-CDs / ABEI (at) Ag nano-enzyme to inhibit the POD-like activity of the nano-enzyme, so that the chemiluminescence intensity generated by oxidation of ABEI is reduced; on the basis, the method is used for detecting glyphosate in a coffee sample, and has the characteristics of high specificity, simplicity in operation and sensitivity.

The invention belongs to the technical field of biochemistry, and discloses a chemiluminescent probe responding to beta amyloid protein as well as a preparation method and application thereof. The structural formula of the probe is shown in the specification. According to the probe designed by the invention, the near-infrared chemiluminescence capability (gt; 650nm) is realized through an extended conjugated structure; meanwhile, high selectivity on A beta is realized by introducing a heteroatom conjugate pi bridge.

The invention relates to the field of antibodies, in particular to an anti-Der p 1 antibody and application thereof. The invention provides an anti-Der p 1 antibody, the anti-Der p 1 antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises an amino acid sequence of a complementarity determining region, and the amino acid sequence of the complementarity determining region comprises CDR1 as shown in SEQ ID No.1, CDR2 as shown in SEQ ID No.2 and CDR3 as shown in SEQ ID No.3; or the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.8. The anti-Der p1 antibody provided by the invention is a monoclonal antibody with high affinity and high sensitivity, after the monoclonal antibody is subjected to humanized IgE transformation, the monoclonal antibody is applied to a Dermatophagoides pteronyssinus allergen component Derp1 specificity IgE magnetic particle chemiluminescence quantitative detection reagent, and a standard curve independently calibrated by the Dermatophagoides pteronyssinus allergen component Derp1 can be obtained; real, accurate and repeatable absolute quantification is realized, the Dermatophagoides pteronyssinus allergen component Derp 1 specificity IgE with the concentration of 0.1 IU / mL can be detected, and the method has clinical diagnosis significance in allergic reaction identification.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

Methods of detecting analytes in a sample are described herein using chemiluminescent labels and branched DNA. Solid supports, reagents, and compounds for use in these methods are also described. Typically, the methods involve specific assay formats which provide the requisite high resolution for detecting low concentrations of analytes such as exosome protein biomarkers in samples.

A method for electrochemiluminescence detection of hydroquinone based on aggregation-induced emission Cd-MOF is provided, including: step 1, preparation of Cd-MOF material; step 2, mixing Cd-MOF material with co-reactant; and step 3, establishment and application of hydroquinone sensor. The organic ligand TPPE with aggregation-induced emission effect and CdCl2 are used to prepare self-luminous Cd-based metal-organic framework Cd-MOF with rigid and directional structure through coordination.

A laryngoscope includes a curved blade including a proximal end, a distal end, and a first channel. The laryngoscope also includes a handle and a second channel. The handle includes a non-electric light source, e.g., a chemiluminescent light source. A catalytic agent in the chemiluminescent light source is activated by pressure on the chemiluminescent light source to generate light that transmits along the first channel. The second channel is disposed proximate and through the blade and configured to provide at least one of oxygen, suction, and an instrumentation into the proximal end and out of the distal end of the blade.

A chemiluminescent nanoconstruct is described herein. The chemiluminescent nanoconstruct structure may comprise a plasmon nanostructure having at least one localized surface plasmon resonance wavelength ([lambda] LSPR), at least one spacer coating, at least one chemiluminescent agent having a maximum emission wavelength ([lambda] EM), and at least one biorecognition element. The signal of the chemiluminescent nanoconstruct structure is greater than the signal of only the at least one chemiluminescent agent.

The application provides a microfluidic chip for SDMA optical chemiluminescence detection and a use method, relates to the field of biomedical detection, and the microfluidic chip comprises a fan-shaped substrate, a sample introduction unit and a diluent unit are arranged on the fan-shaped substrate, a mixing groove is arranged on the side of the diluent unit away from the center of the fan-shaped substrate, and the mixing groove is connected with the sample introduction unit and the diluent unit; a plurality of detection units are arranged on the side of the mixing groove away from the center in a circumferential direction, each detection unit comprises a first reaction groove and a second reaction groove arranged in a radial direction of the fan-shaped substrate and connected with each other, and the first reaction groove is connected with the mixing groove; a first compound and a second compound are arranged in the first reaction groove, and a third compound is arranged in the second reaction groove; by arranging the two reaction grooves, the competition reaction is ensured not to be disturbed by a subsequent signal amplification step in space, so that hook effect is effectively avoided, liquid crosstalk to adjacent detection units is avoided, and the independence of each detection unit and the accuracy of results are ensured.

The invention relates to a kit and a method for detecting phosphorylated Tau protein. In particular to a p-Tau217 protein detection kit and a p-Tau217 protein detection method. The kit and the method disclosed by the invention are based on the p-Tau217 antibody and the total-Tau protein antibody, and are combined with a TSA technology, so that the specificity, the sensitivity and the accuracy of a detection result are improved, and background signals are effectively reduced.

The present application relates to the technical field of nucleic acid detection, in particular to a RNA pathogen chemiluminescence detection method based on nucleic acid structure and sequence characteristics. The present application provides a pathogen RNA rapid detection technology based on the reporter molecule cyclization combined with rolling circle amplification according to the secondary structure and sequence characteristics of RNA, which is suitable for high-sensitivity and high-specificity detection of RNA pathogens such as respiratory viruses. The present application innovatively combines the target structure characteristics with sequence specificity, breaks through the traditional sequence-dependent isothermal amplification technology, realizes the highly specific reporter molecule cyclization, significantly improves the detection reliability, and does not require complex instruments. After the swab sample is collected, the pathogen RNA is rapidly released by using a nucleic acid releasing agent, without complex purification steps, and the reading can be realized through a common chemiluminescence instrument, which is suitable for primary site detection.

The present application relates to a kind of calibration methods for chemiluminescence silicate analyzer, comprising: S1, carries out single-point calibration, comprising: preparation specified concentration silicate standard solution, and obtain original signal value;S2, by multiple calibration stages to chemiluminescence instrument calibration;S1 includes: extract volume X1 of silicate standard solution, concentration C0 ug / L;Extract volume X2 of ultrapure water;Mixing forms specified concentration standard solution;Into chemiluminescence instrument and sequentially add reagent A and reagent B;After waiting, add reagent C, obtain measured voltage V1;S2 includes: first calibration stage, judge V1 whether in a certain range, if yes then enter second calibration stage;If not, adjust until meet;Second calibration stage, take no silicon water and obtain measured voltage V2;Take other concentration silicate standard solution and obtain measured voltage V3;Judge whether |V1-V3|-|V3-V2|≤Voltage fixed threshold value?If meet, then enter third calibration stage;If not, enter fourth calibration stage;Complete calibration.

The invention relates to the technical field of kits, in particular to a magnetic particle chemiluminiscence detection kit for insulin and a preparation method of the magnetic particle chemiluminiscence detection kit for insulin, specifically, a reagent M, a reagent R2 and a reagent R1 are used for forming the kit, and the reagent M is a magnetic particle working solution containing streptavidin magnetic beads; the reagent R2 is an enzyme-labeled working solution containing an insulin antibody labeled by alkaline phosphatase; the reagent R1 is a working solution containing a biotin-labeled insulin monoclonal antibody. Through verification, according to the chemiluminescence kit for insulin provided by the invention, the test sensitivity reaches 2.00 mu IU / mL, the test repeatability for high and low value samples is less than 3%, and the linear correlation with Rogowski reaches 0.98 within the concentration range of 2.00-450 mu IU / mL. The invention also provides a preparation method of the insulin chemiluminescence kit, the production cycle is greatly shortened, the batch difference is easy to control, and the production work of enterprises is facilitated.

The invention discloses a cascade seven-core optical fiber sensor applied to folic acid detection and a preparation method of the cascade seven-core optical fiber sensor, and belongs to the technical field of optical fiber sensing and biological analysis detection. The sensor comprises a first single-mode optical fiber, a seven-core optical fiber section and a second single-mode optical fiber, a double-microsphere-cavity structure is arranged at the optical fiber connecting position to form a cascade interference optical path, and tapering is conducted on the middle portion of the seven-core optical fiber section to form a sensing area. According to the invention, mode matching is improved through the microsphere cavity, an evanescent field is enhanced through tapering, and high-sensitivity specific detection of folic acid is realized in cooperation with the folic acid binding protein biological functional layer fixed on the surface; the sensor obtains stable linear response to a folic acid standard solution in the range of 0-50 ng / mL, a clinical serum sample verifies that the detection result of the sensor is highly consistent with that of a chemiluminescence method, the sensor has good reusability, and the sensor has the advantages of being easy to prepare in structure, rapid in response, high in sensitivity, capable of being matched with a complex matrix and the like, and has good application prospects. The method has huge potential in clinical folic acid quantitative detection.

The invention provides a kit for quantitatively detecting VEGF (Vascular Endothelial Growth Factor) by using a chemiluminescence method as well as a preparation method and a detection method of the kit. The kit comprises a VEGF reagent 1 which is a diluent 1; the VEGF reagent 2 comprises a peroxidase-labeled VEGF antibody and a diluent 2; the VEGF coated plate comprises a VEGF coated antibody, a diluent 1, a diluent 3, a diluent 4 and a chemiluminescent plate; the diluent 1 is a phosphate buffer solution; the diluent 2 is a Tris buffer solution containing fetal calf serum and an HRP enzyme stabilizer; the diluent 3 is a carbonate buffer solution; and the diluent 4 is a phosphate buffer solution containing sodium caseinate and a light-emitting plate stabilizer. According to the detection kit, the double antibody sandwich method immunoassay principle is combined with enzyme labeling and catalytic chemiluminescence immunoassay, the content of the VEGF is quantitatively determined, the detection kit can be prepared with low cost, accurate determination of the VEGF can be achieved, and the detection kit has high sensitivity and good stability.

1. The name of the design product: reagent package (bottle). 2. The use of the design product: for use in chemiluminescence full-automatic analyzer detection. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention relates to a chemiluminescence kit with a modular structure, and belongs to the field of chemical kits, the chemiluminescence kit comprises a supporting seat, a first connecting box is mounted on the supporting seat, a back plate is fixedly arranged on the supporting seat, a clamping assembly is mounted on the back plate, a fixing ring is mounted on the clamping assembly, and a second connecting box is fixedly arranged in the fixing ring; a baffle is fixedly connected to the second connecting box, a connecting assembly and a baffle are installed on the baffle, a rotating shaft and a fixing cylinder are arranged in the middle of the connecting assembly, sampling openings are formed in the two sides of the baffle, and the connecting assembly comprises a rotating block rotationally installed on the rotating shaft. The chemiluminiscence kit solves the problems that an existing chemiluminiscence kit cannot sample and replace a top cover under an anti-pollution condition, does not have an active and repeatable closed anti-pollution sampling function, and cannot be switched between continuous sampling and temporary storage of sampling liquid after sampling.

The invention provides a wireless photochargeable electrochemical luminescence system and device and preparation and application thereof, the system comprises cyano-functionalized CN-K. The device comprises a detachable top camera obscura, a bottom unit, a reactor (containing a functionalized carbon nitride solution integrating solar energy conversion and storage and electrochemical luminescence capability) and a signal acquisition unit. Functionalized carbon nitride is utilized to realize an integrated process of photocharging, electron storage and self-luminescence: the material firstly absorbs sunlight to accumulate long-life electrons, then the accumulated electrons are used as a unique driving force to trigger a surface electrochemical luminescence reaction to realize self-luminescence, and the whole process can be monitored in real time by a signal acquisition unit; a detection signal can be read in real time through equipment such as a smart phone. The wireless self-driven electrochemical light-emitting device has the potential of multi-scene on-site instant detection, and can be applied to visual rapid screening of pollutants of outdoor water.

The invention relates to the technical field of nucleic acid detection, in particular to an RNA pathogen chemiluminescence detection method based on a nucleic acid structure and sequence characteristics. The invention provides a pathogen RNA rapid detection technology which is based on RNA secondary structure and sequence characteristic reporter molecule cyclization and is combined with rolling circle amplification, and is suitable for high-sensitivity and high-specificity detection of RNA pathogens such as respiratory viruses. Target structure characteristics and sequence specificity are creatively combined, a traditional sequence-dependent isothermal amplification technology is broken through, high-specificity reporter molecule cyclization is achieved, the detection reliability is remarkably improved, complex instruments are not needed, after swab samples are collected, pathogen RNA is rapidly released through a nucleic acid releasing agent, complex purification steps are not needed, and the method is suitable for large-scale popularization and application. And reading can be realized through a common chemiluminiscence instrument, and the method is suitable for base-level field detection.