88 results about "Chemiluminescence" patented technology

A laryngoscope includes a curved blade including a proximal end, a distal end, and a first channel. The laryngoscope also includes a handle and a second channel. The handle includes a non-electric light source, e.g., a chemiluminescent light source. A catalytic agent in the chemiluminescent light source is activated by pressure on the chemiluminescent light source to generate light that transmits along the first channel. The second channel is disposed proximate and through the blade and configured to provide at least one of oxygen, suction, and an instrumentation into the proximal end and out of the distal end of the blade.

The application provides a microfluidic chip for SDMA optical chemiluminescence detection and a use method, relates to the field of biomedical detection, and the microfluidic chip comprises a fan-shaped substrate, a sample introduction unit and a diluent unit are arranged on the fan-shaped substrate, a mixing groove is arranged on the side of the diluent unit away from the center of the fan-shaped substrate, and the mixing groove is connected with the sample introduction unit and the diluent unit; a plurality of detection units are arranged on the side of the mixing groove away from the center in a circumferential direction, each detection unit comprises a first reaction groove and a second reaction groove arranged in a radial direction of the fan-shaped substrate and connected with each other, and the first reaction groove is connected with the mixing groove; a first compound and a second compound are arranged in the first reaction groove, and a third compound is arranged in the second reaction groove; by arranging the two reaction grooves, the competition reaction is ensured not to be disturbed by a subsequent signal amplification step in space, so that hook effect is effectively avoided, liquid crosstalk to adjacent detection units is avoided, and the independence of each detection unit and the accuracy of results are ensured.

This invention belongs to the field of organic framework materials technology, specifically disclosing a highly conductive covalent organic framework material containing antiaromatic units and its preparation method. The material is prepared by reacting 2,5-dihydroxy-1,4-benzoquinone (DHBQ) with an o-diamine derivative to form a covalent organic framework (COF) material with a dihydrotetraazapyronine fusion structure. The invention also discloses the applications of this material in electrocatalysis, charge storage, chemiluminescence sensing, displays, and electronic devices.

The present invention relates to a chemiluminescent in vitro diagnostic device using a cartridge having a plurality of wells. The in vitro diagnostic device of the present invention comprises: a syringe having an outlet part at a lower end thereof; a plunger configured to move up and down in a state of being in close contact with the inside of the syringe; a magnet rod attached to the lower portion of the plunger in a height direction and configured such that the lower end thereof can pass through the outlet part of the syringe; a first driving unit for moving the syringe up and down; and a second driving unit for moving the syringe up and down.

The application provides a photochemical chemiluminescence detection reagent and a preparation method thereof, and a procalcitonin detection method. The photochemical chemiluminescence detection reagent comprises acceptor microspheres and donor microspheres; wherein the acceptor microspheres are carboxyl-modified acceptor microspheres, and the carboxyl is activated by sulfo-NHS / EDC and coupled with a procalcitonin antibody; and the donor microspheres are carboxyl-modified donor microspheres, and the carboxyl is activated by sulfo-NHS / EDC and coupled with a procalcitonin antibody. The photochemical chemiluminescence detection reagent has higher stability in various environments, can more accurately combine with target molecules, realizes more efficient detection and recognition, and enables the microspheres to more accurately capture and fix the target molecules, so as to perform subsequent luminescence reaction. The detection method has high sensitivity and a wide range, can be used as a detection reagent for diagnosing and identifying infectious diseases of individuals, and has application value.

This invention provides a quick and simple method for evaluating the thermal degradation susceptibility of polyarylene sulfide (PAS) resin. [Solution] A method for evaluating PAS resin, including evaluating the thermal decomposition behavior from the following rate of change. (However, the heat treatment is a process in which the PAS resin is sufficiently melted at a temperature of melting point + 10°C to melting point + 100°C, and the measurement of the integrated value of chemiluminescence intensity is a method in which the resin is heated from room temperature to 50-200°C under an inert gas atmosphere, then switched to an oxidizing gas atmosphere and held at 50-300°C, and the integrated value of chemiluminescence intensity is measured for a certain period of time immediately after switching to the oxidizing gas atmosphere.) Rate of change (%) = |(integrated value of chemiluminescence intensity after heat treatment / integrated value of chemiluminescence intensity before heat treatment - 1) × 100|

This invention relates to the field of chemiluminescence detection technology for in vitro diagnostic reagents, specifically to an S100-β chemiluminescence detection kit and its preparation method. It provides a chemiluminescence detection kit for central nervous system-specific proteins using magnetic microparticles. The kit comprises: S100-β calibrator, S100-β monoclonal antibody conjugated with carboxyl magnetic beads, S100-β monoclonal antibody labeled with alkaline phosphatase, AMPPD chemiluminescence substrate solution acted upon by alkaline phosphatase, and concentrated washing buffer. This kit achieves the following: no sample pretreatment required, easy operation, high automation, high sensitivity, automatic result output in 16 minutes, good stability of calibrator and enzyme label, and low cost. It can meet the clinical needs for auxiliary diagnosis and treatment of acute ischemic brain injury.

This application discloses a chemiluminescence detection kit for acrylonitrile magnetic microparticles and its detection method. The kit includes a magnetic bead working solution and a luminescent label. The magnetic bead working solution includes acrylonitrile monoclonal antibody A and streptavidin. The luminescent label includes acrylonitrile monoclonal antibody B, modified nano-silica, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N,N-dimethylformamide, acrylonitrile succinimide ester, glutaraldehyde, and ethanolamine. This application uses modified nano-silica as a carrier and grafts a polyethylene glycol hydrophilic layer onto its surface, which amplifies the acrylonitrile ester molecule signal, reduces non-specific adsorption, and improves the sensitivity, specificity, and reliability of the detection.

An in-situ reaction cell adapted for chemiluminescence detection in polymer material reaction processes belongs to the interdisciplinary field of analytical instruments and material aging characterization. This in-situ reaction cell comprises a main body shell, a pull-out sample introduction device, a temperature control system, and a light window cover assembly. The main body of the reaction cell has a reaction cavity and is equipped with inlet and outlet ports to allow for the introduction of various atmospheres. The pull-out sample introduction device includes a sample slot, a pull guide rod, and a pull rod head, enabling stable sample loading and precise positioning. The temperature control system uses a ceramic heating element and a thermocouple working in tandem to achieve precise temperature control. The light window cover assembly adopts a spiral quick-release structure, equipped with a detachable light window and a bandpass filter for easy acquisition of luminescence signals. This invention achieves real-time, in-situ, and highly sensitive monitoring of free radical pathways during polymer reactions, solving the problems of poor adaptability, insufficient accuracy and stability of optical signal acquisition in traditional in-situ reaction cells.

This invention relates to a homogeneous photocatalytic chemiluminescence detection system for detecting HPV vaccine antigen content, its preparation method, and its application. The system utilizes self-made carboxyl-modified donor microspheres and receptor microspheres, coupled with HPV type-specific monoclonal antibodies via EDC / NHS activation, achieving one-step homogeneous detection. By optimizing the microsphere size, a 200 nm microsphere system was found to exhibit higher luminescence signal, a wider detection range, and better sensitivity when detecting HPV16 / 18 VLPs. This method is simple to operate, requires no plate washing, and possesses good specificity, accuracy, and precision. It is suitable for the in vitro relative potency and identification detection of bivalent HPV vaccines, and can replace traditional ELISA methods, reducing detection costs and safety risks. It is also suitable for large-scale applications in vaccine quality control.

This invention relates to a near-infrared low-potential electrochemiluminescence dual-ligand stabilized gold nanoclusters, their preparation method, and applications. The method uses chloroauric acid as the gold source and thiomalic acid and sodium citrate as reducing and stabilizing agents, respectively, in a two-step synthesis. First, Au is reduced online using thiomalic acid. 3+ Water-soluble gold nanoclusters were prepared using a novel method. These nanoclusters were then incubated with sodium citrate, and based on the ligand exchange principle, gold nanoclusters co-coated with thiomalic acid and sodium citrate were synthesized. The raw materials required for this invention are readily available and all possess good water solubility. The synthesis apparatus is simple, the conditions are mild, and the operation is safe. The resulting dual-ligand-coated gold nanoclusters exhibit good stability and produce low-potential near-infrared electrochemiluminescence in the long-wavelength region in an aqueous system. This overcomes the limitation of most current electrochemiluminescent materials requiring hydrazine hydrate or carbazide as a co-reactant. Using a non-toxic tert-butylamine-borane co-reactant, low-potential electrochemiluminescence radiation of 0.57V in the near-infrared region was generated.

The invention provides an iridium metal conjugate, a water-soluble nanoparticle, a preparation method and application in ultrasonic thrombolysis, and belongs to the technical field of sonodynamic therapeutic drugs. The structural formula of the iridium metal conjugate is as shown in formula (I). The invention also provides a preparation method of the iridium metal conjugate. The invention also provides a nanoparticle, and the iridium metal conjugate prepared by the invention has a chemiluminescence property, so that the iridium metal conjugate can realize chemiluminescence imaging-assisted ultrasonic synergistic thrombolysis therapy. The conjugate provided by the invention has a good sonodynamic effect, and can overcome the defect of poor sonodynamic effect required by the existing sonosensitizer. Experiments show that the material has a good treatment effect in thrombolysis treatment.

The application discloses a chemiluminescence kit for detecting CA72-4 based on an acridinium ester system, which comprises magnetic microspheres connected with anti-CA72-4 monoclonal antibody mAb1, an acridinium ester label of anti-CA72-4 monoclonal antibody mAb2, CA72-4 antigen calibrators, a cleaning solution, an excitation solution and the like. The kit is simple to operate, the whole detection time is 20 min, the detection efficiency is greatly improved, the test precision is relatively high, and the CV is not higher than 5%. In addition, the test results of clinical samples show that the kit has a good clinical coincidence rate, and has important significance for the auxiliary diagnosis of various tumors.

This invention belongs to the field of biochemistry technology, and relates to a near-infrared chemiluminescent probe responsive to tau protein, its preparation method, and its application. The preparation method of the near-infrared chemiluminescent probe includes the following steps: adding compound (I), compound (II), a catalyst, and an alkaline additive to a first solvent for reaction to obtain compound (III); adding compound (III), compound (IV), and piperidine to a second solvent for reaction to obtain compound (V); adding compound (V) and trifluoroacetic acid to a third solvent for reaction to obtain compound (VI); and adding compound (VI), compound (VII), and an acid compound to a fourth solvent for reaction to obtain the near-infrared chemiluminescent probe. The chemiluminescent probe of this invention exhibits high selectivity and efficient chemiluminescence capability, providing a promising tool for the diagnosis of Alzheimer's disease (AD). Its structural formula is: [Insert structural formula here].

This invention discloses the application of the serum biomarker miR-206 in the preparation of Alzheimer's disease diagnostic products. This invention is the first to use the serum biomarker miR-206 in the effective and auxiliary diagnosis of Alzheimer's disease, demonstrating extremely high diagnostic performance. This invention lays the technical and data foundation for the development of stable and reliable Alzheimer's disease point-of-care testing kits or automated chemiluminescence detection systems and other in vitro diagnostic products. It has significant clinical application prospects in promoting the transformation of early screening and auxiliary diagnosis of Alzheimer's disease from reliance on complex imaging and cerebrospinal fluid examinations to convenient, non-invasive blood tests.

The utility model relates to the technical field of chemiluminescence detection kit, specifically to the chemiluminescence detection kit of Seneca virus VP1 protein monoclonal antibody, including protection subassembly, the inside of the placing box is provided with clamping assembly, the clamping assembly includes the installation strip, the installation strip side surface is provided with sliding slot, the sliding slot quantity is two and the position corresponds, the sliding groove inner wall sliding connection has the slide, the slide quantity is ten and the adjacent two slides are a group, and the fixed connection has the spring between every group two slides, the slide side surface fixed connection has semicircle clamping plate, the semicircle clamping plate quantity is several and the position corresponds to adjacent two semicircle clamping plates, the installation strip side surface fixed connection is in the placing box inner wall, through the elastic clamping adaptation different specifications sampling tube, prevent it from shaking and falling off in the operation, reduce sample spilling, the protection box body can also block spilled sample when accidentally, guarantee detection.

This invention discloses a chemiluminescence gas sensor based on polyoxometalates (POMs), and relates to its fabrication method and application. The sensor is suitable for detecting ammonia at room temperature. The gas sensor includes an adapter base and a sensing element, wherein the sensing element consists of interdigitated electrodes and a sensitive material loaded on the electrode surface. This sensitive material is a composite material of POMs and SnO2. POMs, as excellent electron acceptors, possess good redox properties, thermal stability, and photosensitivity. When combined with SnO2, they can effectively suppress electron-hole recombination, thereby significantly improving the gas-sensing performance of the semiconductor material. Compared with existing technologies, the sensor provided by this invention exhibits high response characteristics to ammonia at room temperature, while also demonstrating excellent selectivity and repeatability.

The utility model relates to a kind of chemiluminescence imaging instruments, including shell assembly, at least one camera and sample bearing assembly;Shell assembly includes shell, and shell has accommodating cavity and dark cavity, accommodating cavity is communicated with dark cavity by at least one lens hole;At least one camera is located in accommodating cavity, and one camera corresponds one lens hole, and the lens of at least one camera is towards lens hole;Sample bearing assembly is located in dark cavity, and sample bearing assembly includes tray and illumination piece, illumination piece is configured as at least one pair, and each pair of illumination piece is symmetrically arranged about the center line of tray, and illumination piece includes light source.The application is effectively isolated by the separation design of dark cavity and accommodating cavity External light interference, ensure the high sensitivity detection of chemiluminescence signal.And symmetric light source layout improves illumination uniformity, avoid the imaging distortion caused by light intensity difference.

This invention discloses a magnetic microparticle chemiluminescence reagent kit, comprising a box body and a reagent rack, a lower inner liner, and an upper inner liner placed within the box body. The upper inner liner is positioned above the lower inner liner. Both the upper and lower inner liners have reagent wells. The sum of the thicknesses of the upper and lower inner liners is at least 2 / 3 of the height of the reagent rack. This invention, by configuring the inner liners as an upper and lower stacked arrangement, increases the contact area with the reagent rack in its height direction, thereby increasing the stability of the reagent rack within the kit and improving the space utilization of the kit. Furthermore, distributing the reagent wells evenly across the upper and lower inner liners reduces the area of ​​the upper and lower inner liners while still meeting the reagent tube accommodating requirements. The overall volume of the kit is significantly reduced, thereby lowering manufacturing costs and reducing space occupancy during transportation and storage, thus lowering transportation and storage costs.

This utility model discloses a large-spot laser application device, including a device box, and an angle adjustment plate, a laser light source, and a projection plate disposed inside the device box. The two angle adjustment plates are respectively disposed on both sides above the projection plate, and a laser light source is installed on each of the two angle adjustment plates. The irradiation direction of the laser light source is aligned with the projection plate, and the laser light source and the angle adjustment plate are connected to form an adjustable irradiation angle structure. This utility model uses a laser light source to replace the traditional LED light source, halogen light source, and xenon lamp light source, which not only extends the service life of the light source, but also makes the light source smaller and more conducive to the integration of the entire structure. It can be directly applied to the excitation of light sources in CCD camera multicolor fluorescence chemiluminescence gel imaging systems and animal and plant live imaging systems without replacing the light source.

The utility model discloses an automatic calibration device of SCD sulfur chemiluminescence detector. The combustion chamber of detector has first ceramic pipe, connects chromatographic column export, oxygen pipeline and tail blow pipeline, and the inside of first ceramic pipe is equipped with second ceramic pipe and hydrogen pipeline. The automatic calibration device contains sulfide standard gas steel bottle, pressure control valve, six -way sampling valve, desulfurization trap and current -limiting device, and six -way sampling valve is equipped with six interfaces, and tail blow gas inlet is connected with tail blow pipeline, and tail blow gas outlet is connected with first ceramic pipe, and quantitative gas inlet is connected with sulfide standard gas steel bottle through pressure control valve, and quantitative gas outlet is connected with standard gas sample import through quantitative ring, and standard gas sample import is connected with standard gas sample export, and standard gas sample export is connected with desulfurization trap through current -limiting device. The device can improve the detection sensitivity and accuracy, realizes automatic calibration, and is suitable for trace sulfide detection.

This invention relates to the field of chemiluminescence technology for in vitro diagnostic reagents, specifically to a method for preparing a magnetic bead working solution for HCG βCF detection reagent. The method includes diluting the antigen HCGβCF to 2 mg / mL with buffer solution to obtain a working solution; reacting the working solution with a thiol-modified Traut's reagent at 37°C under sealed conditions for 30 min, followed by desalting, purification, and concentration to obtain a reaction solution; diluting the reaction solution to 1 mg / mL with dimethyl sulfoxide aqueous solution, reacting at room temperature for 4 h, and then purifying by changing the solution to obtain an HCGβCF conjugate; and coating the HCGβCF conjugate using any one of SA magnetic bead coupling technology, carboxyl magnetic bead coupling technology, and Tosyl magnetic bead coupling technology to obtain the HCG βCF detection reagent magnetic bead working solution. This invention provides a raw material solution for preparing a low-cost and safe capture antigen, reducing enterprise production costs, reducing biosafety risks during production, improving clinical sensitivity, and improving clinical concordance rates. It solves the biosafety risks associated with the production process of existing HCG antigen-coated carboxyl magnetic bead raw materials.

The present application provides a kind of singlet oxygen activated photo-cage chemiluminescence molecule, which has the structure shown in formula (I), wherein X is hydrogen or halogen, PC represents photo-cage group, R 1 It is an electron-withdrawing group containing oxygen and / or nitrogen, R 2 It is selected from adamantane, cyclobutane, methyl cyclobutyl formate and tetrahydrothiopyran-1,1-dioxide. The chemiluminescence molecule is activated by singlet oxygen and triggered by light of a specific wavelength, and through the processes of oxidation, photolysis, electron transfer, etc., an excited state benzoate structure is generated, which emits blue-green light when returning to the ground state. 2 Group, photo-cage group and R 1 Electron-withdrawing group synergistic effect, oxidation, photolysis, electron transfer, etc. process, generate excited state benzoate structure, it emits blue-green light when returning to the ground state.

The application belongs to the technical field of biological detection, and particularly relates to a kit for detecting platelet aggregation function based on CRET technology and a detection method thereof. The kit comprises an anticoagulant, a platelet aggregation inducer, a donor probe, an acceptor probe, a chemiluminescent substrate, a positive control, a negative control and a buffer. The donor probe is an HRP-labeled CD61 antibody, and the acceptor probe is an amino-modified CdTe quantum dot-labeled PAC-1 antibody or CD62P antibody. The kit based on the CRET technology can realize rapid and accurate detection of platelet aggregation, is particularly suitable for low platelet samples, and is low in cost and anti-interference.

The application discloses a detection method for the affinity of an endogenous opioid peptide to a mu opioid receptor (MOR), a detection kit and application thereof, and is based on a NanoBiT (NanoLuc Binary Technology) luciferase fragment complementation chemiluminescence detection technology, a mu opioid receptor-LgBiT carrier is constructed, a corresponding ligand-HiBiT is synthesized, and the ligand-HiBiT is successfully applied to the determination of the affinity of a beta-endorphin peptide to the mu opioid receptor, so that the mu opioid receptor binding detection at the level of living cells is realized. The detection method and system constructed by the application can be applied to the in-vitro evaluation of the ligand-receptor binding characteristics and the determination of the corresponding endogenous ligand binding affinity, is consistent with the natural mu opioid receptor affinity data determined by a classical method, and is safer and more efficient.

The invention discloses a titanium-doped zirconium MOF molecularly imprinted electrochemical luminescence sensor for high-sensitivity detection of HER2, and a preparation and detection method thereof, and belongs to the technical field of detection. A titanium-doped zirconium-based metal organic framework (Ti-Zr-TPE MOF) is dispensed on the surface of a working electrode to serve as a luminescent material, Ti-Zr-TPE realizes luminous intensity multiplication in an aggregation state compared with Zr-TPE by further enhancing an intra-molecular motion limited (RIM) mechanism, and a luminous body preparation process, co-reactant screening and detection parameters (such as potential scanning rate and pH value) are systematically optimized, so that the luminous intensity of Ti-Zr-TPE is improved. A set of stable Ti (at) Zr-TPE luminescent system is formed. Meanwhile, dopamine (DA) is used as a functional monomer, and HER2 is subjected to molecular imprinting through a molecular imprinting technology, so that the electrochemical luminescence sensor of a quenching system is constructed. The luminescent material based on the novel tetraphenyl ethylene (TPE) derivative has the advantages of high luminescent intensity, low background signal, high stability and specificity, simplicity and convenience in operation and the like, and has obvious advantages compared with a previously developed sensor for detecting HER2. According to the invention, quantitative detection of HER2 can be realized, and an innovative solution is provided for clinical early warning.

This invention discloses a highly sensitive method for detecting troponin I. This method utilizes the reusable nature of a biosensor to perform multiple fluorescence signal reads. Based on these multiple reads, it selects the most accurate concentration range for determining concentrations in the low-to-medium concentration range, achieving higher resolution detection results in both low and medium concentration areas. Furthermore, it incorporates chemiluminescence detection, employing higher-resolution chemiluminescence to determine the final concentration in the high-concentration region. Ultimately, this method achieves better resolution detection results across high, medium, and low concentration regions, enabling accurate identification of sample concentrations across all concentration ranges. It boasts a sensitivity of 0.824 ng / L and a detection range of 0.824 ng / L–100,000 ng / L. The method significantly reduces sample volume, requiring only 20 μL for accurate detection, thus reducing blood volume and improving blood collection efficiency in physical examinations.

Disclosed are kits containing a multiplexed chemiluminescent detection system and microfluidic devices and methods for detecting the presence and / or concentration of biotin and at least one target analyte in a sample. The kits, microfluidic devices, and methods utilize a combination of singlet oxygen-activatable chemiluminescent compounds and two or more fluorescent molecules that emit light at different wavelengths.