87 results about "Phlorizin" patented technology

The invention discloses an extraction process of a high purity phlorizin, comprising the following steps: (1) the raw materials are added with 4-8 times pure water, marinated for 1-3 hours, heated to boiling and micro boiling is kept for 2-3 hours so as to obtain an extracting solution; residue is heated to boiling by using 2-6 times pure water and micro boiling is kept for 2-3 hours so as to obtain a secondary extracting solution; the extracting solutions are merged, cooled and filtered and then the filtered liquid is collected; (2) the filtered liquid is adsorbed by macroporous resin and subjected to gradient elution by using 20 DEG and 50 DEG ethanol; the effluent liquid is collected in subsection, concentrated in vacuum, placed in a quiescence way and cooled, dissolves out crystal at 0-4 DEG C, and dehydrates by high-speed centrifugation; and (3) re-dissolving of pure water, vacuum concentration, re-crystallization, high-speed centrifugation dehydration and drying are carried out so as to obtain the finished product. By adopting pure water for extraction, the organic-residue is decreased and simultaneously the product content is more than 99 percent.

The invention provides a composition of plant extract and a method for regulating and controlling blood sugar by using the same. The composition of a plant extract comprises a glycosidase inhibitor used for blocking digestion and absorption of sugar by organism, and a sodium glucose transporter inhibitor used for inhibiting sodium glucose transporter so as to inhibit the reabsorption of the glucose by kidney. The glycosidase inhibitor comprises L-arabinose, 1-deoxynojirimycin, phaseolin or chlorogenic acid; the sodium glucose transporter inhibitor is extracted from phlorizin; the phlorizin is extracted from lithocarpus polysachyus rehd, branches or leaves of a fruit tree. On the one hand, the composition provided by the invention can be used for blocking excessive absorption of the sugar, on the other hand, the composition can be used for inhibiting the sodium glucose transporter in the kidney to control the reabsorption of the glucose so as to ultimately lower blood sugar and balance the blood glucose level within the scope of health.

The invention discloses an active extract, the active extract is obtained by using ethanol water solution with 5 to 95 percent as a solvent to carry out the extraction from rosaceae and fagaceae plants, the content of phlorizin in the active extract is 30 to 99.5 percent; the invention further discloses a preparation method of the active extract and a usage thereof in the preparation of drugs for treatment for diabetic nephropathy and hepatic edema.

The invention discloses a method for extracting Lithocarpus polystachyus (Wall.) Rehd phlorizin. The method comprises the following steps: carrying out freeze drying on the fresh leaf of the g Lithocarpus polystachyus (Wall.) Rehd in vacuum, and then smashing to obtain the g Lithocarpus polystachyus (Wall.) Rehd powder; adding ethanol and dimethyl carbonate mixing solvent in the powder to perform ultrasonic extraction, centrifugally separating the extracted liquid to obtain supernatant and precipitate; repeatedly ultrasonically extracting the precipitate to respectively obtain the supernatant, and combining the supernatants; and filtering the supernatant through a Millipore filtration, adsorbing through macroporous resin, and finally reducing pressure and evaporating to dryness to obtain the phlorizin. The yield of the Lithocarpus polystachyus (Wall.) Rehd phlorizin extracted through the method disclosed by the invention reaches 90% or more, and the purity reaches 99.8%. The method disclosed by the method has the advantages of simple process, low cost, low energy consumption and simple operation, the industrial production is easy to realize, and the good economic benefit and the good market prospect are obtained.

The invention discloses bacillus velezensis and an application thereof to relieving continuous cropping obstacles of apples. The bacillus velezensis is preserved in China General Microbiological Culture Collection Center on June 10, 2020, and the biological preservation number of the bacillus velezensis is CGMCC NO. 20057. The bacillus velezensis XC1 provided by the invention is a novel biocontrolbacterium having great development potential and has a relatively strong inhibition effect on f. oxysporum, f. moniliforme, f. proliferatum and f. solani, and the inhibition rate of the bacillus velezensis XC1 on the f. oxysporum is the highest and reaches 79.83%. The strain can achieve high yield of antagonistic enzymes such as protease and cellulase; the content of phenolic acid substances suchas phlorizin, phloretin, ferulic acid and cinnamic acid in the soil is reduced; apple tree plant growth is promoted; the method has an excellent effect in relieving continuous cropping obstacles of apple trees, and provides a theoretical basis for biological prevention and treatment of the continuous cropping obstacles of the apple trees.

The invention relates to a method for efficiently extracting phlorizin from plants. The method comprises the steps of crushing dry fruit peels, root barks, roots, leaves and the like of the plants such as apples, pears, litchis and the like which are used as raw materials, and then adding a solvent in a certain proportion; performing pre-treatment by adopting ultrasonic waves, and then performing heat reflux extraction; performing suction filtration, performing decompression concentration on filtrate after adding an antioxidant, adding a flocculant, adjusting the pH value, and then performing suction filtration; cooling the filtrate, concentrating, separating out a crystal, and decolorizing the crystal to obtain a white needle crystal. The method disclosed by the invention is rich in raw material resource, simple in process, high in extraction rate and easy in realization of industrial production.

The invention provides a new application of Chinese flowing crabapple extractive and phlorizin, in particular relating to an application in preparing a drug or a health care food for adjusting estrogen. The Chinese flowing crabapple extractive and phlorizin in the invention has the function of adjusting the estrogen in a two-way manner, definite potency and strong controllability, and provides a new choice for clinic drugs or health care foods.

The invention provides a method for purifying phlorizin from apple velamen, which is simple in process, low in cost and small in pollution, and is suitable for industrial production. The method comprises the following steps of: (1) extraction: smashing apple velamen raw material to obtain smashed material, extracting the smashed material for 1-3 times with water of which the weight is 3-10 times larger than the weight of the apple velamen raw material, and collecting to obtain extract; (2) concentration: concentrating the obtained extract to obtain extractum of which the specific gravity is 0.90-1.08, and filtering to obtain filtrate; (3) acid adjustment: adjusting the pH of the obtained filtrate with acid water to be 2-7, standing, and centrifugalizing to obtain sediment and supernate; and (4) recrystallization: dissolving an obtained phlorizin crude product with 1-10 times of pure water, adding a decoloring agent of which the weight is 1-20% of that of the phlorizin crude product to decolor for 0.5-4 h at the decoloring temperature of 30-80 DEG C, filtering, standing at a room temperature for crystallization, leaching, and drying in vacuum to obtain a phlorizin product.

The invention discloses a method for preparing yellow pigment from phlorizin through two-enzyme process conversion. The method is applicable to preparation of water soluble yellow pigment while phlorizin is taken as a raw material. The method disclosed by the invention comprises the following steps: adding beta-glucosaccharase and polyphenol oxidase into a phlorizin enzymatic oxidation reaction system at the same time, so that part of phlorizin is decomposed into phloretin by the beta-glucosaccharase in advance, and reaction activity is improved; then carrying out enzymatic oxidation under the action of polyphenol oxidase, so that reaction rate and efficiency are greatly improved; and further extracting and purifying, so that the water soluble yellow pigment is obtained. The yellow pigment is bright yellow under the condition that pH is 2-5 and has biological activities such as oxidation resistance. By adopting the method disclosed by the invention for preparing apple yellow pigment, enzymatic conversion rate of phlorizin is effectively improved, and the problem that cost is too high as phloretin is directly adopted as an oxidization substrate raw material is avoided, so that the method disclosed by the invention has a relatively good application prospect.

The invention relates to a technology for semisynthesis of a whitening and anti-skin cancer active ingredient phloretin from natural phlorizin. The technology includes: placing a phlorizin crude product in a stainless steel reaction kettle, dissolving the phlorizin crude product in a 5% strong alkaline solution, conducting simple refining and filtration to obtain a phlorizin salt solution, shifting the phlorizin salt solution into a jacket-equipped enamel reaction kettle, and adding Lewis acid and a metal catalyst at low temperature to let them react for 10-15h at 35DEG C, then lowering the temperature to 0DEG C to perform crystallization, carrying out pumping filtration and deionized water washing to obtain a phloretin coarse grain, then dissolving the coarse grain in a 95% ethanol solution, conducting fine filtration, concentration and cooling crystallization, then performing pumping filtration and drying, thus obtaining a high quality phloretin fine product with content and purity both greater than 99.0%. The technology is suitable for large-scale industrial production.

The invention discloses a degrading bacterium for autotoxic substances of the rhizosphere of an apple tree and an application of the degrading bacterium. The strain is sorted and named Azospirillum lipoferum BL2; the collection unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC); the address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; the collection date is 28th August, 2014; and the collection number is CGMCC No.9619. The strain has good degrading effect on phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid respectively under the condition of liquid culture in a shake flask. After the strain is subjected to liquid shake culture for three days, the result determined by an ultraviolet spectrophotometry shows that the degrading rates of the phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid are as follows respectively: 36%, 34%, 31% and 65%. The result shows that the BL2 strain has good degrading effect respectively on four types of autotoxic substances, and has potential application value for relieving successive cropping obstacles caused by the autotoxic substances.

The invention relates to the field of agricultural microorganisms, and in particular relates to a rhizobium pusense capable of degrading apple autotoxin phlorizin and application thereof. A rhizobiumpusense YIC4105 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 2rd, 2017 with the preservation number of CGMCC No.14852. The bacterial strain is separatedfrom sesbania rhizosphere soil naturally distributed at the Yellow River delta and is obtained through artificial separation and purification; the bacterial strain has relatively strong capability ofdegrading phlorizin and can be stabilized and colonized at rhizosphere of apples; and successive cropping obstacle of an apple seedling caused by accumulation of phlorizin can be effectively preventedby utilizing the bacterial strain, so that the rhizobium provided by the invention has good prospect of being developed into a microbiological inoculants or bacterial fertilizer capable of promotingcrop growth.

The invention provides method for producing a plant cell or plant with increased phlorizin or phloretin glycosyltransferase activity, the method comprising transformation of a plant cell or plant with a polynucleotide encoding a polypeptide with phloretin glycosyltransferase activity. The invention also provides host cells, plant cells and plants, genetically modified to contain and or express the polynucleotides.

The invention provides application of a compound shown in a general formula I in preparing a hepatic or health-care food. The invention also provides a pharmaceutical composition containing the compound shown in the general formula I. The medicament belongs to a pure natural preparation, is safe and reliable, is developed from food, has small toxicity, and has no adverse reaction after being usedfor a long time; the medicament can be drunk as tea, and has convenient use and delicious taste; the medicament has protection effect on hepatic injury caused by various reasons, and has prevention and treatment effects on various diseases caused by the hepatic injury; and the medicament has good effect on treating or preventing acute alcoholism, and can obviously improve various symptoms caused by alcoholic intoxication such as dizziness, headache, hypodynamia and the like.

The invention discloses bacillus licheniformis XNRB-3 and application thereof, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.15308 The strain provided by the invention has the functions of degrading phenolic acid substances such as phlorizin and antagonizing pathogenic bacteria, fermentation metabolites of the strain contain antifungal active substances and substances for promoting plant growth, alpha-bisabolol and 2, 4-di-tert-butylphenol have a remarkable inhibition effect on a plurality of pathogenic fungi, succinic acid has a remarkable inhibition effect on a plurality of pathogenic fungi, and the like. Monomethyl ester and dibutyl phthalate can promote root system elongation and lateral root development of arabidopsis thaliana. The strain can also be used for producing protease, cellulase, chitosanase, beta-1, 3-glucanase and the like; the bacillus subtilis secretes siderophores and auxin and has certain phosphorus dissolving, potassium dissolving and nitrogen fixing capacities. In pot culture and field tests, the addition of the strain XNRB-3 significantly promotes the growth of apple rootstock malus hupehensis seedlings and biennial apple saplings.

The invention discloses a Hubei caval vine general flavone extract which is prepared by the following method: firstly, using ethanol to extract; then using a macroporous resin column or a daiamid column to adsorb; using the ethanol to elute; and collecting the ethanol eluting liquid to carry out vacuum concentration or vacuum drying or spray drying or freeze drying so as to obtain the Hubei caval vine general flavone extract. The content of general flavone in the Hubei caval vine general flavone extract is more than 50 percent, and the total content of phlorizin of a dihydrochalcone compound and 3-hydroxybenzoate phlorizin is more than 40 percent, wherein the mass ratio of the phlorizin to the 3-hydroxybenzoate phlorizin is 20:1-5:1. The obtained Hubei caval vine general flavone extract has low toxicity and can be used for preparing medicaments for treating type II diabetes mellitus.

The invention discloses a method for extracting phlorizin from leaf of lithocarpus polystachyus, which comprises the following steps: (1) grinding the leaf of lithocarpus polystachyus to obtain powder; (2) uniformly mixing powder with the ethanol aqueous solution, putting the mixture into a Soxhlet extractor, obtaining a first extracting solution by performing reflux extraction; (3) filtering thefirst extracting solution to obtain a filter residue and a first extracting solution filtrate, adding the ethanol aqueous solution into the filter residue, and then putting the mixture into a Soxhletextractor, obtaining a second extractive solution by performing reflux extraction; (4) mixing the first extractive solution and the second extractive solution to obtain extractive solution, concentrating the extractive solution, and to obtaining dry powder by freeze drying; (5) static adsorpting statically dry powder in a first chromatographic column and second chromatography column, flushing thesecond chromatography column by the ethanol aqueous solution, evaporating after getting second eluent, and achieving phlorizin dry powder. The method greatly shortens extraction time of organic solvent by reflux extraction of the organic solvent to obtain the phlorizin, and reduces the difficulty of anaphase purification.

The invention relates to a phloretin preparation method, and belongs to the technical field of plant extraction. The method concretely comprises the following steps of (1) poach plant materials with rich phlorizin content with water to obtain water boiling liquid; (2) inoculating green trichoderma spore suspension into the water boiling liquid obtained in the step (1); performing fermentation; after the fermentation is completed, filtering the fermentation liquid to remove thalli; so as to obtain filter liquid; firstly refrigerateing the filter liquid; then, obtaining precipitates from refrigerated filter liquid; next, purifying the precipitates to prepare phloretin solids. The method has the advantages that the operation is simple and convenient; a great number of organic solvents are notneeded; the cost is low; the green and environment-friendly effects are achieved; in addition, the finally prepared phloretin has high yield and high purity; the method is suitable for large-scale production of the phloretin.

Embodiments of the invention provide lithocarpus litseifolius (Hance) Chun electuary and a preparation method thereof. The electuary contains a lithocarpus litseifolius (Hance) Chun extract, a sour agent and dried tangerine peel powder, and is high in effective component content and good in taste. In the electuary, the content of phlorizin reaches 60%, and the content of trilobatin reaches 40%; the trilobatin is a natural sweetening agent, so that the sweet taste of the product can be increased through the enhancement of the trilobatin content, and therefore, the electuary is good for diabetics to take; for non-diabetics, as a common health care product, the electuary can improve memory and maintain the normal level of blood sugar, and has anti-infective effects; and as sweetening agents are not added, weights cannot be increased, so that the electuary is friendly to people who want to lose weights.

The invention provides a method for separating trilobatin and phlorizin from Lithocarpus polystachyus Rehd. The method comprises the following steps: (1) carrying out crushing and extracting; (2) carrying out flocculation, centrifugation and ultrafiltration; (3) carrying out macroporous resin adsorption; (4) removing impurities; (5) carrying out gradient elution: eluting the macroporous adsorptionresin column with low-concentration alcohol and high-concentration alcohol in sequence, and collecting a low-concentration alcohol eluent and a high-concentration alcohol eluent respectively, the low-concentration alcohol containing a small amount of carboxylic acid; (6) carrying out separating and refining of trilobatin; and (7) carrying out separating and refining of phlorizin. According to themethod, two high-content Lithocarpus polystachyus Rehd natural active ingredients can be obtained at the same time, comprehensive utilization of resources is achieved, the process is coherent, operability is high, the equipment requirement is low, the production cost is low, and the method is suitable for industrial production; and the purity and the yield of the obtained trilobatin refined product and phlorizin refined product are ensured and improved.

The invention relates to a phenolic acid degrading bacterium, a biological organic fertilizer and application of the phenolic acid degrading bacterium. Novosphingobium subarcticum AMCC100069 is collected in the China General Microbiological Culture Collection Center (CGMCC) (address: No. 3, Building #1, West Beichen Road, Chaoyang District, Beijing) on July 31, 2015 with a culture collection number CGMCC No.11163. The novosphingobium subarcticum AMCC100069 has a remarkable degrading effect on phenolic acids such as phlorizin causing apple successive cropping obstacles, has a remarkable effect in facilitating growth of fruit trees, and can effectively relieve the problem of apple successive cropping obstacles in production.

The invention relates to a preparation method and quality detecting method of high-purity phlorizin. The preparation method comprises the steps that dried leaf powder of lithocarpus polysachyus (DC.)rehd. is subjected to reflux extraction through ethyl alcohol, extracting solutions are combined and concentrated, suspension is extracted through petroleum ether, then the water liquid part is takenand extracted through ethyl acetate, an ethyl acetate layer is taken and concentrated, and thus an extract is obtained; the ethyl acetate extract is subjected to silica-gel column chromatography, subjected to gradient elution through a methanol-dichloromethane system and detected through thin-layer chromatography, eluent containing the phlorizin is collected and combined, vacuum concentration is conducted, then gel column chromatography is conducted, elution is conducted through a methanol-trichloromethane system, eluent containing the phlorizin is collected, vacuum concentration is conducted,and recrystallization is conducted to obtain phlorizin coarse crystallization; and then separation and purification are conducted through preparation-high performance liquid chromatography (HPLC), the collected eluent is detected through HPLC, and thus the phlorizin with the purity being within 90-98% and a phlorizin composition with the purity being greater than 98% are obtained. According to the preparation method, a technology is simple, the separation speed is high, the production period is short, the obtained product is high in purity, the quality is controllable, and the preparation method is suitable for industrial production.

The invention discloses a method for extracting and separating phloridzin from Lithocarpus polystachyus. The method comprises the following steps: step 1, carrying out cleaning, drying and crushing toobtain Lithocarpus polystachyus powder; step 2, extracting Lithocarpus polystachyus flavonoids to obtain a Lithocarpus polystachyus flavonoid crude extract; step 3, performing secondary separation and extraction on a precipitate in the step 2 to obtain a Lithocarpus polystachyus flavonoid crude extract; step 4, separating and purifying the Lithocarpus polystachyus flavonoid crude extract to obtain a separated product; and step 5, separating and purifying a microporous adsorption resin separated product to finally obtain phloridzin crystalized instant powder. The method for extracting and separating phloridzin from the Lithocarpus polystachyus can extract high-purity Lithocarpus polystachyus phloridzin, only uses water and ethanol in the extraction and separation process as solvents and causes low environmental pollution.

The invention discloses a Docynia delavayi polyphenol extract which has polyphenol content of 91.24 to 91.34% (w / w). The invention also provides a preparation method for the extract. With extraction conditions provided in the invention, polyphenol substances can be maximally extracted from Docynia delavayi, and an extraction rate is as high as 18.54%. High-purity polyphenol and phlorizin can be obtained by further purifying a crude extract with the purifying method provided by the invention. Pharmacological experiments show that the Docynia delavayi polyphenol extract can substantially reduce the levels of cholesterol TC, triglyceride TG, high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in the serum of mice; thus, the Docynia delavayi polyphenol extract can effectively prevent and treat diseases related to abnormal lipids metabolism, such as obesity resulting from abnormal blood fat content, hyperlipidemia, atherosclerosis and coronary heart diseases.

The invention discloses a method for extracting trilobatin and phlorizin from lithocarpus litseifolius. The method comprises the following steps: (1) treating lithocarpus litseifolius leaves to obtainlithocarpus litseifolius leaf powder; (2) mixing the lithocarpus litseifolius leaf powder with an ethanol aqueous solution, and extracting the trilobatin and the phlorizin under an ultrasonic condition; and (3) centrifuging the extracting solution, and taking the supernatant to obtain the trilobatin and the phlorizin. In an extract obtained by the extraction method disclosed by the invention, thecontent of the trilobatin is greater than 162.93 mg / g, the content of the phlorizin is greater than 32.76 mg / g, the antioxidant value of the trilobatin is greater than 53.29%, and the antioxidant value of the phlorizin is greater than 42.90%. According to the extraction method disclosed by the invention, the liquid-to-solid ratio and the ethanol concentration are effectively reduced, the extraction time is shortened, the use amount of an organic solvent is reduced, the extraction efficiency is greatly improved, and besides, the antioxidant values of the trilobatin and the phlorizin are remarkably increased.

The invention relates to application of an apple residue and apple leaf extract to prevention and control of phytopathogen. The apple residue and apple leaf extract contains polyphenols of phlorizin,quercitrin, isoquercitrin, quercetin-3-D-xyloside and quercetin-3-D-glucosidase arabinofuranoside, and can be applied to prevention and control of diseases caused by fungi and diseases caused by bacteria for various grain crops, economic crops, vegetables, fruit trees, melons and the like. A biological sterilizing preparation prepared by extracting a mixture or a single substance is environmentally friendly, is safe to human beings and livestock, and can be used for effectively preventing and controlling various plant diseases.

The invention discloses a strain of Bacillus Velez and its application in alleviating obstacles to continuous cropping of apples. The strain has been preserved in the General Microbiology Center of the China Committee for the Preservation of Microbial Strains on June 10, 2020, and its biological preservation number is : CGMCC NO.20057. Bacillus Velez XC1 provided by the present invention is a novel biocontrol bacterium with great potential for development, and it has relatively good effects on Fusarium oxysporum, Fusarium moniliforme, Fusarium laminarum and Fusarium solani. Strong inhibitory effect, among which the inhibition rate of Fusarium oxysporum is the highest, reaching 79.83%. The strain can also produce high protease, cellulase and other antagonistic enzymes; reduce the content of phenolic acids such as phlorizin, phloretin, ferulic acid and cinnamic acid in the soil; promote the growth of apple tree plants; reduce the obstacles of continuous cropping of apple trees It has excellent effect and provides a theoretical basis for the biological control of apple continuous cropping obstacles.

The invention discloses an application of a magnaporthe grisea gene MoHXT2 in regulation and control of a botanical glycoside transport function. It is found in the invention that a transport proteinencoded by MoHXT2 accepts a plurality of carbohydrates as substrates; namely, the MoHXT2 gene is a hexose transport protein of magnaporthe grisea; after MoHXT2 is knocked out by utilizing a CRISPR / Cas9 gene knockout system, the melanin content of mutant hyphae is found to be obviously reduced, so pathogenicity is greatly reduced; and phlorizin can generate a certain inhibition effect on hexose transportation of MoHXT2, so the growth of magnaporthe grisea is inhibited. Therefore, the MoHXT2 gene can be used for regulating and controlling the botanical glycoside transport function, the pathogenicity of magnaporthe grisea and the growth and development of magnaporthe grisea, can also be used as a drug target of a novel pesticide, and provides a more reliable direction for synthesizing a targeted bactericide.