The invention relates to a cured cross-linked glutinous rice flour hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: glutinous rice flour, a cross-linking agent and water are taken as raw materials, wherein, the cross-linking agent accounts for 0.01%-20% of the glutinous rice flour by mass; and in the presence of a pH regulator, the cured glutinous rice flour acts with the cross-linking agent. The glutinous rice flour is one or more mixtures of sticky rice, sticky corn, husked millet, big glutinous millet, small glutinous millet and sticky sorghum rice and starch and modified starch of the glutinous rice. The cured cross-linked glutinous rice flour hydrogel can be compatible with various matters, drugs of different types and the like, can be used for medical tablets, granules, capsules, gels, ointments, films, implanted preparations and patches as a slowly controlled release film for medicines, an auxiliary material for preparations, a water absorbing gel material and an adhesive material, and can be applied to water absorption, moisture preservation, cosmetics, tissue engineering cell culture, separation and purification, surgical operation, diaphragm and the like.