This application belongs to the field of
biomedicine, specifically relating to a method for preparing an antitumor composition for pets, aiming to solve the technical problems of low
bioavailability, poor stability, and poor
drug administration compliance of poorly soluble active ingredients in antitumor drugs for pets. The method includes: a
raw material pretreatment step, in which
curcumin,
resveratrol,
quercetin,
green tea polyphenols, and
astragalus polysaccharides are pulverized and sieved separately; an organic phase preparation step, in which egg
yolk lecithin and
solid lipids are dissolved in an
ethanol-
acetone mixed
solvent, and
curcumin and
resveratrol are added and dissolved by stirring in a water bath; an aqueous phase preparation step, in which
poloxamer F68 is dissolved in
purified water and dissolved in a water bath; a high-pressure homogenization step, in which a nanoemulsion containing a
solid lipid core is prepared; a moderately polar component encapsulation step, in which
quercetin and
green tea polyphenols are ultrasonically dispersed and then added dropwise to the nanoemulsion; a freeze-
drying step, in which a nano-lyophilized
powder is obtained; and an outer hydrophilic component
coating step, in which
astragalus polysaccharides are sprayed or adsorbed onto the surface of the freeze-dried
powder. The composition obtained by this method is a core-shell multilayer
nanoparticle with a core consisting of
curcumin and
resveratrol encapsulated by
solid lipids, a middle shell adsorbing
quercetin and
green tea polyphenols, and an outer shell coated with
astragalus polysaccharides. This application utilizes the above-mentioned layered encapsulation technology to achieve stable co-encapsulation of multiple antitumor active ingredients, improve the
bioavailability of poorly soluble components, optimize
drug stability, and enhance antitumor effects through multi-target synergistic effects. Simultaneously, it endows the composition with immunomodulatory functions and sustained-release properties. The composition can be formulated into a nano-lyophilized
powder form, making it easy to add to
pet food or formulate into a paste for administration, improving pet
drug compliance. Figure 2 is a
schematic diagram of the three-layer core-shell
nanoparticle structure of the composition of this invention.