415 results about "Cellular organisms" patented technology

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and / or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

Techniques, systems, apparatus and material are disclosed for fabricating a micro-structured biomaterial. In one aspect, a micro-structured biomaterial includes a three-dimensional solid-phase micro-cellular biomaterial that exhibits a negative Poisson ratio that is tunable in magnitude.

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. Particular embodiments include products, product packaging and product labeling. The invention also relates to collars and sleeves for containers, flexible stabilizers for containers, as well as related methods of use.

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and / or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

The invention relates to a new cell biological technique, agent box and prepare device, wherein said invention has high sensitivity, large information amount, simple production, and low cost, while it can analyze different nucleic acids, proteins, and molecules of sample at same time; the invention is characterized in that it adheres different cells on film base or plants them on different micro holes of porous plate, the tested sample is combined with cell or carried biology molecule, and the tested result can be watched by eye or recorded and analyzed by machine. The invention can be used in drug research, biology, etc.

The present disclosure provides a novel mutant strain of Schizochytrium limacinum having the Accession No. MTCC 5249, which produces lipids and extracellular polysaccharide (EPS) simultaneously. The disclosure further provides a process for simultaneous production of lipids and extracellular polysaccharide (EPS) from the novel mutant strain of Schizochytrium limacinum. The lipids produced from the novel mutant strain of Schizochytrium limacinum comprises docosahexaenoic acid (DHA). The disclosure also provides a food, feed, cosmetic, nutritional or therapeutic supplement for humans or animals comprising the cell biomass and extracellular polysaccharides (EPS) of the mutant strain of Schizochytrium limacinum. A cosmetic composition comprising the extracellular polysaccharides (EPS) of Schizochytrium limacinum is also provided that is useful as a base for cosmetics for topical application. The present disclosure further provides a pickle composition and a fat product having improved nutritive value.

The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. Particular embodiments include products, product packaging and product labeling. The invention also relates to collars and sleeves for containers, as well as related methods of use.

The invention relates to the field of cytobiology and in particular relates to a method for preparing platelet lysate and application of the platelet lysate. The preparation method comprises the following steps: oscillating platelets at the temperature of 20-24 DEG C, preserving heat for 1-5 days, treating by adopting a repeated freeze-thaw method and an ultrasonic method, centrifuging, collecting the supernatant, and removing the fibrous proteins, thereby obtaining the platelet lysate. According to the method for preparing the platelet lysate provided by the invention, the content of cell factors in the platelet lysate can be obviously improved, quantitative balance of the cell factors can be realized, the differences among the product batches are reduced, and the effect of stably promoting human-derived cell growth can be achieved.

A biofilm for preparing laminated tissue constructs comprising at least one milk protein forming a sheet like structure can be used to support at least one layer of cells adhered to that. The biofilm allows for a easy handling of cultured cell sheets that can be used as tissue graft. The biofilm, comprising milk proteins and at least one plasticizer, when adhered by a cell layer can form a laminated tissue construct for body tissue replacement.

The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.

The invention provides a preparation method and the application thereof for a cell-biological bracket compound based on biological print technology. The cell-biological compound is formed by fibrous protein which is drawn out from the autoblood of a patient and processed by an ink-jet print technology. The surface and the interior of the cell-biological compound encompass one or more trophic factors and bone mesenchymal stem cells derived from self. According to the invention, the biological print technology is adopted, the appearance shape, cells and encompassing state model of the trophic factors can be designed according to requirements and practical situation, the cell-biological bracket compound is obtained through printing accurately, the fibrous protein and the bone mesenchymal stem cells are derived from self, so that the problem of immunological rejection is avoided, the trophic factors are cultivated in the bracket and are released slowly with the degradation of the bracket material, the cell-biological compound is applied to the spinal cord injured part to promote nerve regeneration and functional reconstruction on the injured part.

The invention relates to the field of accurate medical treatment and cell biology, in particular to a culture method of an in-vitro substance used for scientific research experiments. The method comprises the following steps: 1, treatment of breast cancer tissues from patients: (1) treating fresh breast cancer sample tissues; (2) performing collagenase digestion after shearing; and (3) acquiring breast cancer primary cells; and 2, in-vitro culture of the breast cancer primary cells: (1) mixing the breast cancer primary cells with an organoid culture medium; (2) after solidification, adding thesolidified mixture into a breast cancer organoid culture solution for culture; and (3) acquiring a breast cancer organoid.

The invention provides a device for controlling interaction of various cells as well as a manufacturing method and application of the device. The device provided in the invention comprises a glass substrate (1) and a polydimethylsiloxane seal (2) with a plurality of grooves, wherein the polydimethylsiloxane seal is attached to the surface of the glass substrate to respectively form a plurality of micro-channels, the glass substrate in each micro-channel can be selectively modified to be a substrate for promoting cell adhesion by incubating extracellular matrix proteins or can be modified to be a cell adhesion-resistant substrate by assembling polyethylene glycol solution or is not modified. The device is simple and easy to operate, can control fixation, all movement and partial movement of various cells to achieve the purpose of arbitrarily controlling various cells, can be used for researching the interaction among various cells, the pathology or the cell biological development and can also be used for screening medicines.

The present invention deals with an improved process for the production of high yield of pure Galactooligosaccharides using microbial whole cells in a reactor with cross flow hollow fiber microfiltration system. The process is economical as cell biomass is used repeatedly and eliminated the need to carry out downstream processing for the removal of mono and disacchacrides from the final product.

The invention relates to a CRISPR / Cas12a gene editing system and application thereof. The invention comprehensively utilizes biochemical, molecular biology, cell biology and other methods to screen and identify novel LiCas12a protein with endonuclease activity from a plurality of different bacteria. Based on LiCas12a, a CRISPR / LiCas12a gene editing system with high editing efficiency and high specificity is established in eukaryotic cells and prokaryotic cells separately, and genetic manipulation such as knockout, insertion and point mutation of genes is achieved; a molecular mechanism that the mutation of a TERT gene promoter promotes the cell proliferation of bladder cancer and liver cancer is disclosed. The genetic editing system based on CRISPR-LiCas12a provides a more accurate and efficient gene editing method for research fields such as disease pathogenesis and metabolic engineering transformation.

A method of measuring the biological age of a multicellular organism is disclosed. In one embodiment this method comprises the steps of obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell and determining the expression pattern of a panel of sequences within the nucleic acid that have been predetermined by either increase or decrease in response to biological aging of the organ, tissue or cell. A method of obtaining biomarkers of aging is also disclosed. This method comprises the step of comparing a gene expression profile of a young multicellular organism subject's organ, tissue or cells; a gene expression profile from a chronologically aged subject's organ, tissue or cell; and a gene expression profile from a chronologically aged but biologically younger subject's organ, tissue or cell and identifying gene expression alterations that are observed when comparing the young subjects and the chronologically aged subjects and are not observed or reduced in magnitude when comparing the young subjects and the chronologically aged but biologically younger subjects.

A high throughput toxicology screening method is provided. In the subject method, at least 10 different compound compositions are tested simultaneously. Each compound composition is tested by contacting it with a plurality, e.g. from about 10 to 1000, of non-mammalian multi-cellular organisms and determining the effect of the compound composition on the organisms. The multi-cellular organisms employed in the subject methods are small, have differentiated tissues and organs and have a rapid generation time. The subject high throughput screening methods find use in a variety of applications, and are particularly suited for use in the toxicology screening of libraries of compounds, such as libraries of combinatorially produced compounds.

The present invention belongs to the technical field of cell biology and particularly relates to a technology for regulating and controlling a Jak-Stat pathway to differentiate, dedifferentiate and rejuvenate cells, and an application of the technology. Rejuvenated cell products and / or cell products of different lineage types are obtained by small molecule compound combination, cytokine combination or recombinant protein combination, a gene editing technology and a transgenic technology to quantitatively and / or regularly regulate and control gene or protein targets of the Jak-Stat signaling pathways in cells. The provided technology for regulating and controlling the Jak-Stat signal pathway, and the product / derivatives of the cell product can be used for re-programming cells, tissues, organs and organisms, construct tissue engineering materials, repair damages of tissues and organs of mammals and the aged and degenerated tissues and organs, delay or reverse aging processes of the cells, tissues, organs and organisms, and can be used for immunoregulation of the cells, tissues, organs and organisms.

A photobioreactor is provided for culturing phototrophic microorganisms comprising a plurality of reaction chambers composed of a translucent, pliable, water-impermeable material, each of the reaction chambers being an elongate sleeve capable of holding a culture medium, and a modular support structure comprising a framework defining first and second sides and a first and second end, and configured to support a plurality of horizontally oriented, vertically spaced shelves, each of the shelves extending from the first to the second side of the framework and having disposed thereon one of the plurality of reaction chambers. The design of the photobioreactor is modular, which can be expanded as needed, while maintaining the ratio of a high density of reaction chambers-to-small external footprint. The bioreactor can be used to grow single-celled micro-organisms and other small multi-cellular organisms.

The invention discloses a nucleic acid analysis method based on a constant-temperature cross-catalyzed nuclease reaction. A metastable hairpin in an HCR amplifier is activated by a target to be openedautonomously and continuously; a double-stranded DNA nanowire containing a large amount of DNAzyme is generated; and the DNAzyme continuously catalyzes a shearing substrate to release a large amountof new initiation chains to reversely activate an HCR reaction. Due to the facts that the DNAzyme can continuously accumulate HCR initiation chains and HCR can continuously assemble a large amount ofDNAzyme, the cross-catalytic amplification reaction can convert the molecular recognition process into significantly amplified signals to be output. The R-HCR amplifier can be easily combined with anauxiliary sensing module to be developed into a universal sensing system, and ultra-sensitive in-vitro detection and intracellular imaging of single-molecule miRNA are successfully achieved. The nucleic acid analysis method provided by the invention has wide application prospect in the aspect of in-vivo detection of trace biomarkers in cytobiology and clinical diagnosis.

The invention provides a cell-biomaterial composite support frame. The composite support frame comprises a support frame base body and viscous cell suspension capable of slowly releasing cells, wherein the composite support frame forms the AC<n>B distribution formation from bottom to top, wherein the A is a support frame base body layer; the B is a viscous cell suspension layer; the C is a mixing layer formed by the viscous cell suspension through crossed distribution in support frame base body gaps; the n is a positive integer from 3 to 20; the viscous cell suspension comprises cells, cell carrying carriers and water; and the carriers are biocompatible viscous materials. The distribution density of the ells on the composite support frame is controllable; the adherence rate and the cell survival rate are high; and the problems of weak tissue regeneration promoting capability, weak proliferation and differentiation and slow growth of the cells on a three-dimensional composite support frame can be solved. The invention also provides a preparation method and application of the composite support frame.

A method of producing a thermo-stable biodegradable thermo-stable biomatrix for storage of biological materials in disclosed. The biomatrix contains a bio-polymer selected from the group of xanthan gum, acacia gum, guar gum, gellan, starch or a combination therof. The biological material can be a wide range of materials including; a bio-inoculant such as Rhizobium, or any microorganism or cellular organism. The biomatrix can be formulated for fast or slow release of the biological materials and maintains activity of the biological materials after being stored for months. In use the biomatrix can be applied in the form of a pellet or mixed with liquid and applied to seeds, plants or soil.

Provided herein are methods to increase the culture density and / or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and / or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and / or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.

A method of determining structural data of a prototype for a lightweight technical structure by selecting shell architectures of natural bio-mineralized unicellular organisms in accordance with an aspect set very closely adapted to the lightweight structure to be produced and by directly copying and thereafter scaling their structural data to the prototype before optimizing the Prototype by perfect adaptation to the lightweight structure to be produced.

The invention discloses a preparation method of a biosensor for detecting L-cysteine and an application of the biosensor in detecting yield of escherichia coli L-cysteine. Main components of the biosensor comprise a transcriptional control gene CcdR coding gene from pantoea ananatis, a promoter region and a terminator region of the transcriptional control gene CcdR coding gene, a promoter region of a gene ccdA from pantoea ananatis, a green fluorescent protein coding gene eGFP and a plasmid framework pTrc-Mob. The biosensor can have a good linear relation under the L-cysteine concentration of 0-32 mmol L<-1> and has the highest fluorescence intensity value and cysteine concentration relation under the L-cysteine concentration of 0.01-8 mmol L<-1>. Through combination with a high-throughput screening system, the biosensor performs real-time detection on the yield of escherichia coli L-cysteine, can screen out high-yield mutants of L-cysteine and mutants for key enzymes in biological synthesis of L-cysteine. Specific, quantitative and real-time detection of L-cysteine can be realized, and the sensitivity is high.

The invention relates to the field of gene engineering and cytobiology, in particular to a preparation method for efficiently amplifying NK cells by utilizing trophoblasts. The construction method comprises the following steps: (1) constructing a pHR-mbIL-21 plasmid vector, a pHR-4-1BBL plasmid vector and a pHR-MICA plasmid vector; (2) preparing recombinant lentiviruses by using pHR-mbIL-21, pHR-4-1BBL and pHR-MICA plasmid vectors respectively; (3) preparing K562-mbIL-21-4-1BBL-MICA trophoblasts; (4) extracting PBMC cells; and (5) carrying out in-vitro amplification on NK cells. The inventionprovides the preparation method of NK cells. The method employs the step of independently constructing plasmid vectors for expressing mbIL-21, 4-1BBL and MICA molecules, K562 is infected with recombinant lentivirus, the NK cells can be prepared, the preparation method overcomes the defects in the prior art, the K562 cells simultaneously expressed by IL-21, 4-1BBL and MICA molecules are used as trophoblasts for amplification culture of the NK cells, the amplification multiple of the NK cells reaches up to 890 times, the purity of the prepared NK cells reaches 92.2%, and the repeatability of theamplification multiple between different PBMC cells is good.