161 results about "Cellular organisms" patented technology

A compound, as shown in Formula I, is a multi-heterocyclic compound. It has been verified that the compound provided by this invention integrates a novel compound targeting the calcitonin gene-related peptide (CGRP) based on ephedrine. Upon contact with the skin, due to photoactivated cell biological function regulation and the blocking effect of the multi-heterocyclic compound I structure on serotonin receptors, it achieves therapeutic effects on cutaneous vascular malformations and telangiectasia, improves the effectiveness of phototherapy in improving chronic cutaneous vascular diseases, and promotes skin wound healing.

The present invention relates to a cell biomass (e.g. food product or pet food product) or its components prepared from cultivated metazoan cells, method of its preparation and a system for its preparation.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention discloses a multi-layer heterogeneous network unicellular organism network inference method based on meta-path enhancement, which mainly comprises a gene regulation knowledge base enhanced multi-layer heterogeneous network construction module for integrating an external gene interaction network and multiple omics data such as scRNA-seq, scATAC-seq, ST and the like; constructing a single-cell multi-omics multilayer heterogeneous network containing cell-cell, cell-gene and gene-gene relationships, and fusing spatial constraints to consider cell positions and tissue structures; and the feature enhancement module based on the meta-path explores complex semantics of the network by designing a multi-hop meta-path mode, designs an adaptive multi-view learning framework and a multi-round enhancement mechanism, and optimizes feature representation by using cell-gene interaction and cross-modal attention fusion. The unicellular biological network can be effectively deduced, the deduction accuracy and biological interpretation are remarkably improved, the method plays an important role in understanding the cell biological process, developing and treating diseases and the like, has good expandability, and can further integrate multi-modal omics data such as proteomics and metabonomics.

The invention discloses a tissue dissociation liquid, a tissue dissociation method and application thereof, and relates to the technical field of cell biology. In a DMEM / F12 culture medium, the tissue dissociation liquid only contains the following raw materials in concentration: 0.5 to 3 mg / mL of type II collagenase, 0.5 to 2 mg / mL of type IV collagenase, 0.5 to 2 mg / mL of hyaluronidase, 10 to 18 [mu] M of Y27632 and 80 to 150 [mu] g / mL of primocin. The tissue dissociation liquid provided by the invention can dissociate normal tissues or tumor tissues into single cells or cell clusters, the single cells obtained by the digestion method are high in proliferation rate and easy to successfully culture, and the tissue dissociation liquid has the technical advantages that the cell yield and survival rate are maintained at a relatively high level. Therefore, the tissue dissociation liquid provided by the invention has good application prospects in tumor tissue dissociation, cell line construction and organoid model construction.

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

The present application provides a kind of block cell culture meat and its preparation method.The present application meets the demand of seed cell large-scale preparation of block cell culture meat by suspension cell scale culture, and realizes the adhesion, proliferation and integration of seed cell on edible scaffold material by specific culture medium induction of suspension cell adhering to wall.The method can be in stirred tank cell bioreactor by sterile scaffold material and cooperate with specific induction culture solution and stirring process, so that the adhesion rate of cell and scaffold material reaches 96.8%.The method uses suspension cell as seed cell, and combines corresponding process and culture medium, effectively solves the problems of seed cell difficult to be large-scale prepared and difficult to be effectively integrated with scaffold material in the culture process of block cell culture meat, and provides scalable scheme for industrialized production of block cell culture meat.

The invention discloses a method for regulating and controlling the growth of mammary epithelial cells of milk goats based on a KLC1-chi-miR-423-5p-PDGFAceRNA network, and belongs to the field of animal molecular biology and cell biology. Through combination of transcriptome sequencing and functional verification, it is revealed for the first time that KLC1 can be used as ceRNA, and targeted inhibition of chi-miR-423-5p on PDGFA mRNA is effectively relieved through competitive combination of chi-miR-423-5p, so that proliferation of mammary epithelial cells is promoted, and apoptosis is inhibited. The research results of the invention not only fill the blank of research on the regulation and control mechanism of the breast epithelial cell ceRNA network of the milk goat, but also provide a theoretical basis for breast development regulation and control, tissue repair and prevention and treatment of related diseases, and lay an important foundation for developing a potential molecular intervention strategy.

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention discloses a cell culture medium supplement as well as a preparation method and application thereof, and relates to the technical field of cell biology. The supplement comprises DL-alpha-tocopheryl acetate, linolenic acid, ethanolamine, L-carnitine hydrochloride, 3, 3 ', 5-triiodoL-sodium thyrosinate, and serum albumin. As a cell culture additive with definite chemical components and simple composition, the cell culture additive can replace a traditional culture system with unknown chemical components or too complex composition, and is used for supporting proliferation, differentiation and characterization analysis of cells. The culture medium additive is clear in chemical component, simple to prepare, stable in effect and relatively low in cost, can promote in-vitro proliferation and oxidation resistance of cells including human embryonic stem cells and mesenchymal stem cells, and has important application value in actual production.

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

The invention relates to the technical field of medicines, in particular to application of a neuropeptide Y1 receptor antagonist in preparation of a hereditary polycystic kidney disease treatment medicine. The neuropeptide Y1 receptor antagonist is selected from a compound BIBO3304 which plays a role in blocking combination of neuropeptide Y (NPY) and a Y1 receptor subtype thereof. On the basis that early-stage histopathology, high-throughput sequencing, cytobiology and molecular biology are combined with in-vitro cell culture and in-vivo animal experiments, the potential treatment effect of the neuropeptide Y1 receptor antagonist in preparation of hereditary polycystic kidney disease treatment drugs is provided, and a new basis is provided for hereditary polycystic kidney disease treatment.

The application discloses a molecular genetic marker affecting pig sexual maturity and application thereof. The application takes RBP1 as a research object, and adopts molecular and cell biology methods to study the correlation between RBP1 and the initial estrus period. It is found that six SNP sites in the RBP1 promoter region are related to the initial estrus period. Further research shows that the promoter activity of the CC genotype of the g.80408109T>C site is significantly higher than that of the TT genotype, the promoter activity of the AA genotype of the g.80409632A>G site is significantly higher than that of the GG genotype, and it is confirmed that RBP1 can inhibit the ferroptosis of granulosa cells. It can be seen that the mutation in the RBP1 promoter region can affect the expression of RBP1, and RBP1 can accelerate the initial estrus start by inhibiting the ferroptosis of pig ovarian granulosa cells. The application has good application value for the research on ovarian follicle development and initial estrus start.

The invention discloses a tylosin sensing biosensor, a construction method thereof and MphR mutant protein based on the tylosin sensing biosensor, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to detect tylosin. In order to solve the technical problem, the invention provides the biosensor for sensing tylosin, the biosensor can express an MphR31D1 protein and / or a coding gene containing the MphR31D1 protein, and the MphR31D1 protein can be a protein with an amino acid sequence of SEQ ID NO: 1. The invention also provides a preparation method of the biosensor, and application of the MphR31D1 protein and related biological materials thereof in detection of tylosin. According to the invention, escherichia coli Top10 is taken as a chassis bacterium, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, and the tylosin whole-cell biosensor is successfully constructed through protein directed evolution.

The utility model relates to the technical field of animal tissue slicing, and discloses an adjustable animal tissue slicing device which comprises a main body shell, a driving module is arranged on the side wall of the main body shell, a conveying piece is fixedly connected to the inner side wall of the main body shell, and a transverse groove is formed in the conveying piece. On the premise of an automatic cutting mechanism, the slicing precision and uniformity can be improved, and the problem of non-uniform slicing thickness caused by human factors such as hand shaking and non-uniform force application in manual operation is effectively avoided; the thickness of each time of cutting can be accurately controlled, the consistency of the thickness of the slices is ensured, and a highly reliable and repeatable data basis is provided for subsequent histological analysis, pathological diagnosis and cell biology research, so that the accuracy and reliability of experimental results are improved, experimental errors caused by the thickness difference of the slices are reduced, and the experimental efficiency is improved. And scientific researchers can more accurately reveal the microstructure and pathological change characteristics of tissue cells.

The invention provides a multi-parameter flow cytometry detection method and system for ferroptosis phenotype quantification, and relates to the technical field of cell biological detection and analysis. By setting a ferroptosis induction control group, a ferroptosis inhibition control group and a group to be detected, lipid peroxidation signals, free iron signals and membrane integrity signals are synchronously collected in the same detection process, and original multi-parameter data are obtained. And performing spectrum unmixing and batch correction by utilizing artificial intelligence, and screening a target event set based on a preset event quality control condition. On the basis, the ferroptosis probability of the event to be detected is calculated, the ferroptosis phenotype index is determined, standardization and quantification of ferroptosis phenotypes are achieved, and the method is suitable for high-throughput screening and clinical evaluation.

The present application relates to a method for inducing liver oval cell line to form functional liver-like organ tissue on a liver acellular biological scaffold, belonging to the field of biotechnology, wherein the liver oval cell line (HOCL) is used as seed stem cells, and is implanted into a regenerative liver acellular biological scaffold (R-DLS) with biological activity; according to the in-vitro simulation of the development of a body-like liver organ microenvironment concept, a conditional culture solution capable of inducing HOCL liver function differentiation and reconstructing a functional liver organ structure is added, and a functional liver-like organ tissue is constructed in an in-vitro 3D culture system. In the method, the HOCL in the R-DLS shows its differentiation into functional hepatocytes in 2-24h in the in-vitro 3D culture system, and the liver-like organ tissue can be formed in 21 days. The "new liver" constructed by the method has important clinical conversion value for being used as a liver transplantation donor and treating ESLD.

The utility model provides a cryopreservation box with a programmed cooling function, and relates to the technical field of cryopreservation boxes. The cryopreservation box with the programmed cooling function comprises a box body, a cover body and a containing assembly, the cover body is hinged to the box body, the containing assembly is arranged in the box body and movably connected with the inner wall of the box body, a liquid storage cavity is formed between the bottom face of the containing assembly and the inner bottom face of the box body, and the liquid storage cavity is communicated with the box body. The box body is provided with a liquid injection port and a liquid discharge port which are communicated with the liquid storage cavity; a cooling assembly is further arranged on the outer wall of the box body and comprises a condensing box, an air extracting pump, an air extracting pipe and an air returning pipe, the air extracting pump is connected with the condensing box and the air extracting pipe, the other end of the air extracting pipe is communicated with the interior of the box body, and the air returning pipe is connected with the condensing box. The two ends of the air return pipe communicate with the interior of the condensation box and the interior of the box body correspondingly. And the cells can be directly cryopreserved after being cooled, so that the influence on the biological activity of the cells is reduced.

This application discloses a method and apparatus for optimizing a bioreaction process using deep learning, relating to the field of cell bioengineering. The method includes acquiring the first bioreaction type, the first reaction stage, the first basic configuration parameters of the cell bioreactor, and the first monitoring parameters collected by multiple types of sensors in the current bioreaction process within a cell bioreactor; retrieving contextual data related to the first bioreaction type and the first reaction stage from a bioreaction knowledge vector base; constructing prompt words based on the contextual data, the first monitoring parameters, the first bioreaction type, the first reaction stage, the first basic configuration parameters, preset data analysis instructions, and historical question-and-answer data from monitoring and analysis; and inputting the prompt words into a trained large-scale cell bioreaction model, so that the large-scale cell bioreaction model outputs first control parameters for a programmable logic controller to predict the operating state of the cell bioreactor and correct for faults.

The invention belongs to the technical field of biomedical treatment, and particularly relates to recombinant human fibronectin max-rhFN as well as a preparation method and application thereof. The amino acid sequence of the recombinant human fibronectin mat-rhFN is as shown in SEQ ID NO. 2. The molecular weight of the recombinant human fibronectin mat-rhFN provided by the invention is about 130kDa, the purity is higher than 95%, and the endotoxin content is relatively low. Through cell biology detection, the recombinant human fibronectin mat-rhFN provided by the invention is good in activity, has the effects of promoting cell proliferation and cell adhesion, and also has a good protection effect in a keratinocyte anti-SDS stimulation test, and meanwhile, the recombinant human fibronectin mat-rhFN provided by the invention has a remarkable interaction with collagen.

The invention relates to the technical field of cytobiology and regenerative medicine, and discloses a composition and method for efficiently and directionally inducing iPSC (induced pluripotent stem cells) to be differentiated into functional microglial cells, and the method comprises the following steps: S1, digesting the induced pluripotent stem cells into single cells, resuspending the single cells by using an embryoid induction culture medium, inoculating the single cells into a low-adsorption culture plate, and culturing for 2-4 days to obtain a culture medium; forming an embryoid; s2, transferring the obtained embryoid into a matrigel-coated culture plate, adding a myeloid precursor cell induction culture medium for culturing, regularly changing the liquid during the period, and culturing for 2-3 weeks to obtain suspended myeloid precursor cells; s3, collecting the obtained myeloid precursor cells, centrifuging the myeloid precursor cells, re-suspending the myeloid precursor cells by using a microglial cell end induction culture medium, inoculating the myeloid precursor cells into a culture plate, adding the microglial cell end induction culture medium for culturing, regularly changing the medium during the culture period, and culturing for 10-14 days to obtain mature microglial cells. The invention solves the problems of long period and high cost of the existing scheme for differentiating the iPSC into the microglial cells.

This invention discloses the application of a Cav3.2 inhibitor in the preparation of a drug for treating intervertebral disc degeneration. Z944 can block the activation level of Cav3.2 in nucleus pulposus cells, effectively alleviating lipid metabolism disorders and cellular biological behavior disorders induced by abnormal mechanical stress, and ultimately reducing intervertebral disc degeneration. This demonstrates that Z944 is a drug with clinical application potential, which can be used to alleviate the pain and burden of patients with low back pain and improve their quality of life.

The present invention relates to the production of culture medium comprising a co-hydrolysis of plant- based protein source by microorganisms. The invention further relates to the use of this culture medium for the cultivation of non-human metazoan cells used as a consumable product for human and / or animal consumption. Further, the invention provides novel methods for repurposing waste molecules and by- products associated with preparation of culture medium and cultivation of non-human metazoan cells.

The invention belongs to the technical field of nano medicine, and particularly discloses a method for obtaining exosomes with different cell biological functions, which comprises the following steps: preparing hybrid cells HCs integrating different cell functions by a cell fusion method, and further collecting exosomes H / Exos derived from the hybrid cells. The invention also discloses the exosome and application of the exosome in preparation of drugs. Different hybrid cells have multiple functions of cells before fusion, and exosomes secreted by the hybrid cells inherit the functions of the hybrid cells. The hybrid cells can continuously secrete exosomes with the characteristics of the two parent cells in the proliferation process. The defect that the end-to-end differentiated cells cannot be proliferated is overcome, so that large-scale preparation of the natural multifunctional exosome can be realized, and a new method is provided for customized exosome preparation for disease treatment.

The present invention relates to a method of synthesizing a (poly)peptide or protein of interest (POI) in a cell-free protein synthesis (CFPS) reaction mixture. The present invention further relates to a cell-free biological system which is or comprises a CFPS reaction mixture as defined or used in accordance with the method of the invention. The present invention also relates to the respective CFPS reaction mixture, and CFPS buffer / buffer composition.

The application belongs to the technical field of cell biology, and provides an oocyte in vitro maturation culture medium additive and application thereof. The experimental results of the application show that coumarin 106 can improve the meiosis process blockage of oocytes, reduce the morphological proportion of abnormal spindle and the proportion of misaligned chromosomes, restore the level of acetylated tubulin, improve microtubule stability, improve the expression level of actin in the spindle of oocytes, improve the misdistribution and expression decrease of mitochondria in oocytes, improve the level of mitochondrial membrane potential, improve the fluorescence signal of cortical granules and specific cortical granule proteases in oocytes, improve the fertilization rate, reduce the level of reactive oxygen species and DNA damage in oocytes, and reduce the occurrence of early apoptosis in oocytes. The culture medium prepared by using coumarin 106 can be used for promoting oocyte in vitro maturation.

Immune cells are separated out through human peripheral blood, a byproduct supernatant is obtained in the process of culturing the immune cells in a GMP laboratory, the supernatant serves as one of main components of a finished product according to existing known science and technology, biological natural laws and special effects, a substrate solution and dressing mode is adopted as an external carrier for skin, and the external carrier is used as an external carrier for the skin. According to the present invention, the immune cell supernatant is applied to the skin surface so as to obtain the method of introducing various cell bioactive components from the immune cell supernatant and acting on the skin surface to the deep layer, such that the skin care product with characteristics of skin cell activation moisturizing, regeneration repairing and physiological aging delaying can be provided, and the significant multi-effect purpose can be provided; and the technical method of biological products can be organized and produced under specific GMP production conditions.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

The invention belongs to the technical field of cell biology, and particularly relates to a triple co-culture cell model for respiratory function research as well as a construction method and application of the triple co-culture cell model. Based on BEAS-2B, THP-1 and MRC-5 cells, a triple co-culture cell model is constructed by adopting a Transwell three-dimensional construction mode, and a complex cell interaction environment after human respiratory tract tissues are exposed to harmful factors such as heavy metals and the like can be simulated. The constructed model is closer to a real physiological anatomical structure in structure, and the multicellular synergistic toxic reaction caused by heavy metal exposure is more comprehensively reproduced in a functional level; an integrated in-vitro model which is closer to human lung tissue in structure, covers multi-stage reaction in function, is standard and stable in operation and has multi-dimensional analysis capability in mechanism is realized for the first time, scientificity, sensitivity and practical applicability of research on respiratory system toxicity caused by heavy metal are remarkably improved, and the method has relatively high technical advancement and industrial transformation potential.