387 results about "Cellular organisms" patented technology

Techniques, systems, apparatus and material are disclosed for fabricating a micro-structured biomaterial. In one aspect, a micro-structured biomaterial includes a three-dimensional solid-phase micro-cellular biomaterial that exhibits a negative Poisson ratio that is tunable in magnitude.

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. Particular embodiments include products, product packaging and product labeling. The invention also relates to collars and sleeves for containers, flexible stabilizers for containers, as well as related methods of use.

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

A biofilm for preparing laminated tissue constructs comprising at least one milk protein forming a sheet like structure can be used to support at least one layer of cells adhered to that. The biofilm allows for a easy handling of cultured cell sheets that can be used as tissue graft. The biofilm, comprising milk proteins and at least one plasticizer, when adhered by a cell layer can form a laminated tissue construct for body tissue replacement.

The invention relates to the field of accurate medical treatment and cell biology, in particular to a culture method of an in-vitro substance used for scientific research experiments. The method comprises the following steps: 1, treatment of breast cancer tissues from patients: (1) treating fresh breast cancer sample tissues; (2) performing collagenase digestion after shearing; and (3) acquiring breast cancer primary cells; and 2, in-vitro culture of the breast cancer primary cells: (1) mixing the breast cancer primary cells with an organoid culture medium; (2) after solidification, adding thesolidified mixture into a breast cancer organoid culture solution for culture; and (3) acquiring a breast cancer organoid.

The invention provides a device for controlling interaction of various cells as well as a manufacturing method and application of the device. The device provided in the invention comprises a glass substrate (1) and a polydimethylsiloxane seal (2) with a plurality of grooves, wherein the polydimethylsiloxane seal is attached to the surface of the glass substrate to respectively form a plurality of micro-channels, the glass substrate in each micro-channel can be selectively modified to be a substrate for promoting cell adhesion by incubating extracellular matrix proteins or can be modified to be a cell adhesion-resistant substrate by assembling polyethylene glycol solution or is not modified. The device is simple and easy to operate, can control fixation, all movement and partial movement of various cells to achieve the purpose of arbitrarily controlling various cells, can be used for researching the interaction among various cells, the pathology or the cell biological development and can also be used for screening medicines.

The invention relates to a CRISPR/Cas12a gene editing system and application thereof. The invention comprehensively utilizes biochemical, molecular biology, cell biology and other methods to screen and identify novel LiCas12a protein with endonuclease activity from a plurality of different bacteria. Based on LiCas12a, a CRISPR/LiCas12a gene editing system with high editing efficiency and high specificity is established in eukaryotic cells and prokaryotic cells separately, and genetic manipulation such as knockout, insertion and point mutation of genes is achieved; a molecular mechanism that the mutation of a TERT gene promoter promotes the cell proliferation of bladder cancer and liver cancer is disclosed. The genetic editing system based on CRISPR-LiCas12a provides a more accurate and efficient gene editing method for research fields such as disease pathogenesis and metabolic engineering transformation.

A method of measuring the biological age of a multicellular organism is disclosed. In one embodiment this method comprises the steps of obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell and determining the expression pattern of a panel of sequences within the nucleic acid that have been predetermined by either increase or decrease in response to biological aging of the organ, tissue or cell. A method of obtaining biomarkers of aging is also disclosed. This method comprises the step of comparing a gene expression profile of a young multicellular organism subject's organ, tissue or cells; a gene expression profile from a chronologically aged subject's organ, tissue or cell; and a gene expression profile from a chronologically aged but biologically younger subject's organ, tissue or cell and identifying gene expression alterations that are observed when comparing the young subjects and the chronologically aged subjects and are not observed or reduced in magnitude when comparing the young subjects and the chronologically aged but biologically younger subjects.

The present invention belongs to the technical field of cell biology and particularly relates to a technology for regulating and controlling a Jak-Stat pathway to differentiate, dedifferentiate and rejuvenate cells, and an application of the technology. Rejuvenated cell products and/or cell products of different lineage types are obtained by small molecule compound combination, cytokine combination or recombinant protein combination, a gene editing technology and a transgenic technology to quantitatively and/or regularly regulate and control gene or protein targets of the Jak-Stat signaling pathways in cells. The provided technology for regulating and controlling the Jak-Stat signal pathway, and the product/derivatives of the cell product can be used for re-programming cells, tissues, organs and organisms, construct tissue engineering materials, repair damages of tissues and organs of mammals and the aged and degenerated tissues and organs, delay or reverse aging processes of the cells, tissues, organs and organisms, and can be used for immunoregulation of the cells, tissues, organs and organisms.

A photobioreactor is provided for culturing phototrophic microorganisms comprising a plurality of reaction chambers composed of a translucent, pliable, water-impermeable material, each of the reaction chambers being an elongate sleeve capable of holding a culture medium, and a modular support structure comprising a framework defining first and second sides and a first and second end, and configured to support a plurality of horizontally oriented, vertically spaced shelves, each of the shelves extending from the first to the second side of the framework and having disposed thereon one of the plurality of reaction chambers. The design of the photobioreactor is modular, which can be expanded as needed, while maintaining the ratio of a high density of reaction chambers-to-small external footprint. The bioreactor can be used to grow single-celled micro-organisms and other small multi-cellular organisms.

A method of producing a thermo-stable biodegradable thermo-stable biomatrix for storage of biological materials in disclosed. The biomatrix contains a bio-polymer selected from the group of xanthan gum, acacia gum, guar gum, gellan, starch or a combination therof. The biological material can be a wide range of materials including; a bio-inoculant such as Rhizobium, or any microorganism or cellular organism. The biomatrix can be formulated for fast or slow release of the biological materials and maintains activity of the biological materials after being stored for months. In use the biomatrix can be applied in the form of a pellet or mixed with liquid and applied to seeds, plants or soil.

Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.

The invention discloses a preparation method of a biosensor for detecting L-cysteine and an application of the biosensor in detecting yield of escherichia coli L-cysteine. Main components of the biosensor comprise a transcriptional control gene CcdR coding gene from pantoea ananatis, a promoter region and a terminator region of the transcriptional control gene CcdR coding gene, a promoter region of a gene ccdA from pantoea ananatis, a green fluorescent protein coding gene eGFP and a plasmid framework pTrc-Mob. The biosensor can have a good linear relation under the L-cysteine concentration of 0-32 mmol L<-1> and has the highest fluorescence intensity value and cysteine concentration relation under the L-cysteine concentration of 0.01-8 mmol L<-1>. Through combination with a high-throughput screening system, the biosensor performs real-time detection on the yield of escherichia coli L-cysteine, can screen out high-yield mutants of L-cysteine and mutants for key enzymes in biological synthesis of L-cysteine. Specific, quantitative and real-time detection of L-cysteine can be realized, and the sensitivity is high.

The invention relates to a cell micro system for detecting the mark material at the surface of cell and relative detecting method, wherein said system comprises a micro current control detecting chip, a light source, a convex lens, a lens, a camera, a computer, and a tested cell driver; said light source, convex lens, micro current control detecting chip and lens are arranged on the system base from up to down; the center of light source and the centers of convex lens, lens and reflective lens are in one line; said camera is connected to a computer; the computer is connected to the tested cell driver. Since the system comprises a micro current control detecting chip and the chip has micro current groove, the driving pump can pump sample cell into micro current groove to directly detect the mark material. And the system can avoid special external device; and the camera is connected to the computer, to online or off-line analyze the test process.

The invention discloses a multifunctional graphene gene vector construction method, wherein small-size graphene quantum dot with fluorescence is used as a basic vector, and is functionally linked to acationic polymer branched chain polyethyleneimine so as to achieve gene transfection ability, and the non-gene-transfection cationic polymer part can be tracked due to the fluoresce ability. According to the present invention, the synthesis process is simple, the constructed multifunctional graphene gene vector has advantages of low toxicity and high transfection efficiency, the highest transfection efficiency can achieve 80.57%, gene transfection and tracking can be simultaneously achieved, and the constructed multifunctional graphene gene vector can be used for cell biology research and gene therapy field.