14 results about "Disposable bioreactors" patented technology

The application discloses a kind of disposable bioreactor and method for preparing adenovirus harvest, belong to biological preparation technical field, the bioreactor includes reaction bag and magnetic stirring mechanism, the magnetic stirring mechanism includes stirring shaft, stirring vane, the number of the stirring vane is greater than 1 and is installed on stirring shaft in layers, the stirring vane includes second stirring vane and first stirring vane, the second stirring vane is fixed on stirring shaft, the first stirring vane is installed on stirring shaft by collar, further include linkage module and shear force transmission device, the linkage module is used to drive shear force transmission device action, realize shear force transmission device and collar connection and disengage.The method is realized based on the bioreactor.Adopting the present scheme, the stirring consistency of disposable bioreactor can be effectively guaranteed in the process of being used for expansion culture.

This application relates to the field of bioculture technology, and in particular to a disposable bioculture bag. A disposable bioculture bag includes a main body, a cone, and an electrode mounting structure. The cone has a first diameter end and a second diameter end, with the inner diameter of the first diameter end being larger than the inner diameter of the second diameter end and connected to the main body. A first through hole is formed on the side wall of the cone. The electrode mounting structure is located outside the cone and includes a base and a mounting tube. A second through hole is formed on the base, which fits against the side wall of the cone, and the first and second through holes correspond and communicate with each other. One end of the mounting tube is mounted on the base and communicates with the second through hole. The end of the mounting tube connected to the base faces the second diameter end, and the angle between the axis of the mounting tube and the side wall of the cone is acute. By providing an electrode mounting structure mounted on the side wall of the cone of the disposable bioculture bag, this application can reduce electrode wear and make the collected data more accurate.

1. Name of the designed product: Disposable bioreactor controller (ZenMac). 2. Use of the designed product: For controlling the culture process of bioreactor. 3. Design points of the designed product: In shape. 4. Picture or photo best indicating the design points: Perspective view 1.

A bioreactor system (1) has a pre-sterilized single-use bioreactor (2) with a reactor wall (20) surrounding an interior chamber (21) that receives a fluid medium (M). A sensor (3) for detecting an analyte in the medium has a sensor housing (30) with an outside thread (31) and a sensor shaft (32), a distal end portion (33) of which has an end face (34) with a region (35) permeable to the analyte. A sensor receptacle (4) connected to the reactor wall receives the sensor, to maintain the sterility of the single-use bioreactor. A circumferential flange (40) fixes the sensor receptacle to the reactor wall. The flange has an inside thread (42) that receives the outside thread. A wall (43) of the sensor receptacle is connected to the flange, and together with the sensor shaft, protrudes into the interior chamber, separating the sensor from the interior chamber.

The utility model relates to the technical field of cell bioreactors, in particular to a flexible stirring system suitable for a disposable biological reaction bag, which comprises a flexible shaft and a rigid shaft, the flexible shaft is an axially hollow flexible sleeve, a paddle is mounted on the flexible shaft, the flexible shaft penetrates through the reaction bag, two ends of the flexible shaft are respectively connected with the reaction bag, and the flexible shaft can rotate in the reaction bag; the rigid shaft is inserted into the flexible shaft, the rigid shaft is used for inputting stirring power and is separated from the interior of the reaction bag through the flexible shaft, and when the rigid shaft rotates, the flexible shaft and the paddles are driven to rotate synchronously.

The utility model discloses a disposable biological reaction membrane, which relates to the technical field of biological reaction membranes and comprises a membrane body with six layers. The thickness of the substrate is 100 [mu] m; wherein the top layer is a contact layer; the contact layer is made of ultra-low density polyethylene; the bottom of the contact layer and the waterproof layer are integrally arranged through the second nylon layer. The side, away from the second nylon layer, of the waterproof layer and the blocking layer are integrally arranged. One side far away from the waterproof layer is integrated with the bottom layer through a first nylon layer; by controlling the thickness of the membrane body, extractable substances and immersions in the membrane body are effectively reduced when organisms react, so that the stability of the organisms when the organisms react on the reaction membrane is effectively ensured, and external substances entering a biological reaction system are effectively avoided through the barrier layer and the waterproof layer.

The utility model relates to the field of microbial culture, in particular to a disposable bioreactor, which comprises a tank body, a flexible container and a mixing system, the mixing system comprises a stirring shaft attached to the flexible container, the upper end part of the stirring shaft is connected with a hub, the hub is provided with at least three groups of guy cable supporting structures, and the guy cable supporting structures are connected with the tank body. The guy cable supporting structure comprises a guy cable, a welding disc and a fastener, and one end of the guy cable is fixed to the hub. The guy rope starts from the hub and sequentially penetrates through the welding disc and the fastening piece. The guy cable and the welding disc are integrally formed, the welding disc is fixed to the side wall of the flexible container in a sealed mode, and the guy cable is fixed to the tank body through the fastener. According to the utility model, the upper end part of the stirring shaft is fixed at a corresponding position through the guy cable supporting structure with certain strength, and a supporting force is provided for the upper end part, so that the stirring shaft is not easy to shake in the rotating process; meanwhile, the integrated attachment structure of the guy and the flexible container reduces the risks of fluid leakage and accidental contamination, and packaging, storage and installation of the flexible container are facilitated.

The invention relates to a method of transferring a microbial bioprocess from a multi-use bioreactor (1) to a single-use bioreactor (2), wherein the source bioprocess is a batch or fed-batch microbial bioprocess and the source bioprocess arrangement (3) comprises a source bioreactor (5) with a stirrer and the source bioreactor (5) is the multi-use bioreactor (1) which has at least 1000 L production volume of a microbial culture producing a maximum heat of at least 25,000 W and / or the stirrer of the source bioreactor (5) is driven with a maximum torque of at least 120 Nm, wherein the target bioprocess is a continuous microbial bioprocess and the target bioprocess arrangement (4) comprises a target bioreactor (6) and the target bioreactor (6) is the single-use bioreactor (2) which has a production volume of a microbial culture of at most 50 % of the production volume of the source bioreactor (5) and of at most 800 L, the microbial culture of the target bioreactor (6) produces a maximum heat of at most 20,000 W during the target bioprocess and / or the target bioreactor (6) comprises a stirrer driven with a maximum torque of at most 100 Nm, wherein an average amount of the bioproduct produced per time of performance of the target bioprocess by the target bioreactor (6) is at least 90 %, preferably at least 100 %, of an average amount of the bioproduct produced per time of performance of the source bioprocess by the source bioreactor (5).

The utility model belongs to the technical field of biological experiment equipment, and particularly relates to a disposable biological reaction bag support which comprises a mounting frame, connecting lugs are fixedly arranged on the two sides of the mounting frame, a connecting block is fixedly arranged on the inner wall of the top of the mounting frame, and four inserting holes are formed in the outer portion of the connecting block. Through the arrangement of the sleeve, the positioning hole, the limiting groove, the telescopic rod, the storage hole, the second spring, the guide plate, the positioning rod and the hook, the positioning rod is extruded inwards, the positioning rod extrudes the guide plate, after the positioning rod completely retracts into the storage hole, the telescopic rod can be pulled, the telescopic rod drives the hook and the positioning rod, and when the positioning rod moves to the position of the positioning hole, the hook can be pulled out. A second spring pushes a guide plate upwards, then the guide plate drives a positioning rod to be popped out of a positioning hole, positioning of a telescopic rod is achieved, when the position of a hook is matched with the biological reaction bag, adjustment can be stopped, the adjustable telescopic rod can be suitable for biological reaction bags of different sizes, and the practicability of the equipment is improved.

The application discloses a preparation method of particles for preparing a liquid contact layer, characterized in that the method comprises the following steps: adding metallocene polyethylene and fumed silica into a blender respectively for stirring to obtain a mixture; and adding the mixture into an extruder for hot melt extrusion and underwater cutting to obtain the particles for preparing the liquid contact layer. In addition, the application also discloses a disposable bioreactor membrane material prepared from the particles. The disposable bioreactor membrane material has the advantages of high tensile strength, good barrier property, high transparency and good flexibility.

The present invention discloses a method for inducing callus tissue of high-flavonoid-yielding alpine edelweiss and culturing it in reactor suspension. The present invention mainly aims at the technical defects of the prior art, and provides a method for stably obtaining callus tissue of high-flavonoid-yielding alpine edelweiss and conducting large-scale reactor culture, so as to solve the problem that there is currently no alpine edelweiss cell line with high flavonoid content. The present invention establishes a method for inducing callus of alpine edelweiss by conducting a large number of screening and comparison of basic elements and hormones in the culture medium and exploring other experimental conditions, inducing callus tissue with the stems and leaves of sterile seedlings, and establishing a tissue culture scheme for culturing alpine edelweiss cell lines that can produce high-flavonoid compounds, and can use disposable bioreactors to amplify step by step to mass-produce high-flavonoid-yielding alpine edelweiss cells.

A Disposable Bioprocess System of a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. Also, a method for operating the sterile Disposable Bioprocess System in perfusion mode for continuously processing.

The invention provides a disposable bioreactor bag, and belongs to the field of bioengineering. Comprising a reaction bag; the film microporous membrane is arranged on the reaction bag, and a plurality of air holes are formed in the film microporous membrane; the stirring assembly is mounted in the reaction bag, and one end, far away from the reaction bag, of the stirring assembly faces the microporous membrane; the inoculation supplementing device is mounted at the upper end of the reaction bag; the gas inlet device is mounted on the reaction bag and is used for filling reaction gas into the reaction bag; the exhaust device is mounted on the reaction bag and is used for exhausting gas in the reaction bag. The invention aims to solve the problem of unstable structure when a gas introduction device is connected with a reaction bag in the prior art. The device has the technical effects that the installation difficulty is reduced through direct welding of the microporous membrane and the reaction bag membrane material.