2522 results about "Biological materials" patented technology

The invention relates to the technical field of slope disaster prevention and control, in particular to a slope disaster prevention and control management method and system for a hard mountainous area. The method comprises the following steps: acquiring a holographic feature map of a mountain slope; performing stress field analysis on the mountainous area slope based on the mountainous area slope holographic feature map to obtain a mountainous area slope stress field; performing freeze-thaw sensitive area identification on the mountainous area slope based on the mountainous area slope stress field to obtain a slope freeze-thaw risk distribution map; according to the slope freezing and thawing risk distribution map, microenvironment simulation regulation and control are conducted on the mountain slope, and a slope microenvironment regulation and control scheme is obtained; carrying out biological material adaptability analysis on the mountainous area slope to obtain a slope ecological matrix distribution diagram; and according to the slope ecological matrix distribution diagram, laying anchoring points on the mountainous area slope to obtain a mountainous area slope anchoring network. According to the invention, the prevention accuracy of freeze-thaw disasters is improved.

The invention belongs to the technical field of optimized coding genes, and discloses a recombinant human I-type collagen as well as a preparation method and application thereof, and the amino acid sequence of the recombinant human I-type collagen is shown as SEQ ID No.1. The recombinant I-type collagen provided by the invention is relatively high in expression quantity, has relatively good cell migration promoting, cell proliferation promoting and cell adhesion promoting properties, is high in safety, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention discloses an application of a TaNLP-3B gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaNLP-3B is cloned from wheat, the gene TaNLP-3B is overexpressed in wheat Fielder to obtain a TaNLP-3B transgenic overexpressed plant, and a detection result shows that the protein content in wheat seeds can be remarkably increased by more than 40% through TaNLP-3B gene overexpression, so that the TaNLP-3B gene has an effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be increased by more than 40%. The method can be applied to plant breeding or quality improvement.

The invention provides a high-stability recombinant XVII type collagen as well as a construction method and application thereof, and belongs to the technical field of bioengineering. According to the invention, an amino acid sequence of natural human (XVII) type collagen is selected as a basic unit and repeated for multiple times to obtain recombinant (XVII) type collagen; in the design process of the recombinant XVII type collagen, an unstable GXY triad is avoided, potential matrix metalloproteinase restriction enzyme cutting sites and glycosylation sites are abandoned, a KGD active sequence is reserved, and the probability of containing sites which are easy to degrade can be reduced; a sequence fragment of the recombinant XVII type collagen is derived from a full-length sequence of a natural collagen gene, has excellent solubility, biological activity and high stability, and can be kept stable in a high-temperature storage environment and under the condition of avoiding alkalinity; the product can be used as a raw material in the production process of medicines, medical equipment, biological materials or cosmetics, and has good practicability.

The invention discloses a biological material mechanical testing method and system based on finite element analysis, and is used for the technical field of data processing method.The biological material mechanical testing method comprises the following steps that a three-dimensional geometric model of a biological material is constructed, the three-dimensional geometric model of the biological material is imported into finite element analysis software, and the finite element analysis software is used for analyzing the three-dimensional geometric model of the biological material; grid division is executed, and material attributes and boundary conditions are set; finite element simulation is operated according to the set material attributes and boundary conditions, stress and strain distribution is analyzed, and grid division and the material attributes are adjusted based on the analysis result; and the adjusted grid division and material attributes are simulated again, a simulation result is verified through experimental data, and meanwhile material composition is adjusted based on the simulation result and a verification result. According to the method, each step can be completed according to preset time and quality standards, and deep understanding of the simulation process and prevention of potential problems are enhanced.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention discloses a glucose response type insulin delivery system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The glucose response type insulin delivery system provided by the invention comprises a delivery carrier and a bioactive component encapsulated in the carrier, the delivery carrier is a metal polyphenol network formed by carrying out coordination reaction on epigallocatechin gallate and metal ions; the bioactive components comprise glucose oxidase, catalase and insulin. The glucose response type insulin delivery system provided by the invention can adjust the release of insulin according to the concentration of glucose in the surrounding environment, so that the blood glucose is maintained at a normal level.

The invention discloses a plant leaf imitating hydrogel composite material and a preparation method thereof, and belongs to the field of bionic composite materials. The preparation method comprises the following steps: firstly, preparing printing paste by compounding a coloring agent, printing a fabric to obtain a green fabric capable of simulating spectral characteristics of green peaks and red edges of plant leaves, and simulating fence tissues and skeletons in a leaf structure by using the green fabric; then taking a biological material polyvinyl alcohol (PVA) as a base material, adding a monomer with strong hygroscopicity, a cross-linking agent and an initiator, and blending to prepare a hydrogel prepolymer solution; and finally, pouring the hydrogel prepolymer solution into a mold containing the green fabric for copolymerizing, so that hydrogel is directly formed on the surface of the fabric and in pores, and the plant leaf imitating hydrogel composite material is obtained. The material gets rid of dependence on moisture absorption salt, and has the advantages of stable water absorption and simulation effects, good durability and the like.

The invention relates to a silk fibroin biological material with high beta-fold content and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: preparing a regenerated silk fibroin solution; adding an enzyme catalytic promoter and an enzyme into the regenerated silk fibroin solution for incubation; the enzyme catalysis auxiliary agent is selected from one or more of a metal ion catalyst, a zwitterionic surfactant, a functionalized ionic liquid and a calcium phosphate nano-cluster; and centrifuging, cleaning and drying the obtained product to obtain the silk fibroin biological material with high beta-fold content. The silk fibroin material has higher beta-folding amount through primary structure modification, the space structure is more crystallized, and the amino acid sequence is more regular and ordered. The silk fibroin material provided by the invention has excellent mechanical properties, efficiency and yield, and is a biological protein material with great development potential.

The invention belongs to the field of biological materials, and particularly relates to recombinant V-type humanized collagen and application thereof. The invention provides a recombinant V-type humanized collagen, the amino acid sequence of the recombinant V-type humanized collagen comprises (repetitive unit) n, and the repetitive unit comprises a sequence as shown in SEQ ID NO.1; each repeating unit is directly linked and the number n of repeating units is 10. The recombinant V-type humanized collagen can comprehensively resist skin aging.

The invention discloses an application of a TaPKMT-5A gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaPKMT-5A is cloned from wheat, the gene TaPKMT-5A is overexpressed in wheat Fielder, a TaPKMT-5A gene overexpressed plant is obtained, and a detection result shows that the protein content in wheat seeds can be remarkably increased by 13.3%-16.9% or above through TaPKMT-5A gene overexpression, so that the TaPKMT-5A gene has the effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be remarkably increased by 13.3%-16.9% or above. The method can be applied to plant breeding or quality improvement.

The invention belongs to the field of synthetic biology, and particularly relates to collagen fusion polypeptide as well as application and a preparation method thereof. The collagen fusion polypeptide provided by the invention comprises: (a) a first polypeptide of recombinant III-type collagen; and (b) a second polypeptide comprising a trimerized folding domain. The collagen fusion polypeptide provided by the invention has the advantages of low immunogenicity, good biocompatibility, high biological activity and the like, can significantly promote proliferation and migration of human skin fibroblasts, and has stronger ability of promoting regeneration of endogenous collagen. The collagen fusion polypeptide has a triple helix structure similar to natural collagen, and can be self-assembled to form collagenous fibers which are good in morphology and similar to natural collagen. The method can be widely applied to the fields of skin repair dressings, biological materials, artificial implants, medical instruments, cosmetics, health care products, food and the like.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention relates to the technical field of synthetic biology, in particular to recombinant XVII type collagen as well as a preparation method and application thereof.The amino acid sequence of the recombinant XVII type collagen comprises a sequence shown in any one of SEQ ID NO.1-SEQ ID NO.3. The recombinant XVII type collagen can be highly expressed in an escherichia coli and pichia pastoris system, the expression system is simple, the cost is low, and the recombinant XVII type collagen is suitable for industrial production. The yield is high; the biological activity is good. The protein can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines, so that the protein can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention provides recombinant VIII type humanized collagen and application thereof, and the recombinant VIII type humanized collagen is of a triple helix structure, has good cell adhesion activity, does not generate immunological rejection and anaphylactic reaction when being applied to a human body, is a brand new human body synthetic biological material, and has good application prospects.

The invention provides an application of a transporter in improving the yield and / or purity of lactose-N-neotetraose (LNnT), and relates to the technical field of genetic engineering. Transporter proteins of different sources are screened, the transporter protein which aims at LNnT synthesis and can achieve efficient transportation is obtained, and a new biological material is provided for LNnT synthesis. Moreover, the efficient transporter obtained by screening is used for transporting the LNnT to the outside of cells, so that the pressure on the cells caused by overhigh concentration of intracellular products is relieved, the conversion of an intermediate product LNT II to the LNnT is improved to the maximum extent, the yield is improved while byproducts are reduced, the yield of a byproduct lactose-N-neohexaose is also reduced, and the separation and purification of later products are facilitated.

The invention provides a fiber dressing for promoting wound healing and a preparation method and application thereof, and belongs to the technical field of biological materials. The shell of the fiber dressing contains the first medicine for promoting polarization of macrophages, and the first medicine can promote polarization of macrophages at normal and diabetic wound surfaces from M1 type to M2 type; a shell substrate is a mixture of hydrophilic and hydrophobic polymers, so that painless adhesion and stripping of a wound surface can be realized by absorbing seepage and spraying non-irritant normal saline on the wound surface, and secondary injury of the wound surface is reduced; an inner core contains a second medicine and a third medicine which are used for inducing death of M1 type macrophages, so that wound inflammatory response can be reduced, wound bacteria can be killed, wound infection degree can be reduced, and wound healing can be accelerated; the core matrix is a mixture of hydrophilic and hydrophobic polymers with good biocompatibility, can provide mechanical support for the fiber dressing, and provides a slow release effect for the two substances in the core. By controlling the composition of the inner core and the shell, the healing of normal wounds and diabetic wounds can be effectively promoted.

The invention belongs to the technical field of bioengineering, and particularly discloses a chimeric enzyme for degrading PET (Polyethylene Terephthalate) as well as a related biological material and application of the chimeric enzyme. The chimeric enzyme for degrading PET provided by the invention is obtained by replacing at least one fragment in an amino acid sequence as shown in SEQ ID No.1-12 on the basis of IsPETase. Compared with IsPETase, the chimeric enzyme for degrading PET provided by the invention has the advantages that the thermal melting temperature is increased by 4.8-31.7 DEG C, the concentration of a degradation product TPA after PET degradation is increased by 1.5-44.5 times, while the application conditions of PET degradation are widened, the PET degradation efficiency can be greatly improved, the recycling rate of the degradation product TPA with high added value is increased, and the chimeric enzyme has important economic value and environmental benefit. The method can be used in the fields of PET degradation, PET degradation agent preparation or PET degradation product preparation and the like.

The invention relates to the technical field of biological materials, in particular to a hydrogel scaffold material for spinal cord injury repair as well as a preparation method and application of the hydrogel scaffold material. The hydrogel scaffold material AP39 (AP39-coated LIP) for spinal cord injury repair is prepared by combining the liposome-coated AP39 (AP39-coated LIP) serving as a drug with a GelMA hydrogel scaffold, and a scaffold is provided for injured spinal cords, so that axon regeneration of injured parts is guided, mitochondria of the injured parts is targeted to release H2S, neuronal injury is relieved, and the spinal cord injury repair effect is improved. Therefore, nerve regeneration and functional recovery after spinal cord injury are promoted.

The invention discloses cytosine deaminase as well as a related biological material and application thereof, and belongs to the technical field of protein. The technical problem to be solved by the invention is to excavate natural cytidine deaminase without sequence preference and improve the base editing efficiency and range. The invention provides a 15th-143rd site protein of which the amino acid sequence is SEQ ID NO.2. The invention also provides a preparation method of the protein. The invention also provides application of the protein and related biological materials thereof in single base editing. According to the application, metagenome big data is utilized, comprehensive and systematic information mining analysis is carried out on novel cytidine deaminase, and a protein L85 which is highly similar to a known DddA protein structure but low in sequence similarity is selected from the novel cytidine deaminase for functional verification. The protein L85 has high catalytic activity as cytidine deaminase, is not limited by target sequence preference, can be widely applied to gene editing in vitro and in cells / tissues, and has good application potential and development value.

The TaSL1 protein or the biological material provided by the invention has the functions of improving the grain number and the ear length of wheat, and after the expression of the TaSL1 protein in wheat is reduced, the grain number, the ear length and the awn length of the obtained transgenic wheat are remarkably increased compared with those of a contrast. The TaSL1 protein or a biological material provides a new gene resource for cultivating wheat varieties with multiple grains per ear and long ears, and has important significance for improving the wheat yield.

A device and method for delivering biological material and / or synthetic chemistries to a liquid medium comprising securing a capsule to an opening of a container, the capsule comprising a cap portion for securing the capsule to the container and an elongate portion comprising a cavity defined by a frangible bottom, wherein the cavity comprises live biological material therein and a plunger comprising a disrupting mechanism, suspending the elongate portion of the capsule into an interior cavity of the container, depressing the plunger, cutting or disrupting the frangible bottom of the capsule, and releasing the material into the liquid medium.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention relates to the technical field of medical biological materials, in particular to an aquatic animal source acellular matrix and a preparation method and application thereof. The aquatic animal source acellular matrix is prepared by performing pretreatment and acellular treatment on connective tissues of aquatic animals; the pretreatment method comprises the following steps: carrying out enzymolysis for 12-60 hours by using biological enzyme at the temperature of 0-15 DEG C, and then removing muscular tissues, basilar membranes and epidermal layers to obtain the miscellaneous tissue-free dermal tissues. The aquatic animal-derived acellular matrix provided by the invention has a bionic structure similar to or consistent with animal original tissues, does not contain components possibly causing immune risks, and is high in clinical safety and effectiveness.

A microfluidic device, a microfluidic system comprising said microfluidic device, and to a method of culturing biological material.

The invention discloses application of an LRK20 gene in regulation and control of rice disease resistance, a related biological material and a cultivation method. According to the application of the LRK20 gene in regulation and control of rice disease resistance, the sequence of the LRK20 gene is shown as SEQ ID NO.1, and the disease resistance is sheath blight resistance. The amino acid sequence of the protein coded by the LRK20 gene is as shown in SEQ ID NO. 2. The recombinant vector comprises the complementary sequence of the LRK20 gene in the application. The novel gene LRK20 for regulating and controlling the resistance of the rice sheath blight disease is obtained through identification, the sheath blight resistance gene LRK20 can regulate and control the resistance of the rice sheath blight disease, the resistance of the rice to the sheath blight disease can be remarkably weakened by knocking out the gene, and the resistance of the rice to the sheath blight disease can be remarkably enhanced by overexpressing the gene; therefore, the gene has a potential breeding application value in sheath blight resistance molecular breeding.

Microparticles for detecting biological materials are provided. Each of the microparticles includes: a core-shell structured microparticle consisting of a core including a magnetically responsive metal and a shell layer surrounding the core and having a uniform thickness; and capture probes introduced onto the shell layer to capture biological materials.

The invention discloses a preparation method of biological breeding pork rice, and belongs to the technical field of biological breeding meat. The preparation method of the biological breeding pork rice comprises a preparation method of pork breeding cereals, collagen incubation, pig myoblast inoculation, pig myoblast culture and pork rice harvesting. According to the technical scheme, the production process is mature and controllable, and stable product quality can be kept in large-scale production; the production process does not depend on a microcarrier, the production cost is reduced, the production process is simplified, and the whole production process is more efficient and economical; by using biological materials such as fish collagen protein and hyaluronic acid, the nutritional value and biocompatibility of the product are improved, so that the final product has higher market competitiveness; the production process is green, environment-friendly, safe, reliable and resource-saving; the cereal and new protein combined pork rice provides a new possibility for consumers to select in the food market.

The invention provides a hydrolyzed extracellular matrix and a preparation method and application thereof, and relates to the technical field of biological materials.The hydrolyzed extracellular matrix prepared through the method comprises hydrolyzed collagen, hydrolyzed elastin, hydrolyzed fibronectin and hydrolyzed laminin, in the hydrolyzed extracellular matrix, the number of polypeptide molecules with the molecular weight of 500-3000 Daltons accounts for not less than 96%, a good transdermal absorption effect can be achieved, and the hydrolyzed extracellular matrix has a good effect of promoting cell growth and proliferation and also has good functions of resisting wrinkles and enhancing skin elasticity.