1882 results about "Biological materials" patented technology

The invention relates to the technical field of slope disaster prevention and control, in particular to a slope disaster prevention and control management method and system for a hard mountainous area. The method comprises the following steps: acquiring a holographic feature map of a mountain slope; performing stress field analysis on the mountainous area slope based on the mountainous area slope holographic feature map to obtain a mountainous area slope stress field; performing freeze-thaw sensitive area identification on the mountainous area slope based on the mountainous area slope stress field to obtain a slope freeze-thaw risk distribution map; according to the slope freezing and thawing risk distribution map, microenvironment simulation regulation and control are conducted on the mountain slope, and a slope microenvironment regulation and control scheme is obtained; carrying out biological material adaptability analysis on the mountainous area slope to obtain a slope ecological matrix distribution diagram; and according to the slope ecological matrix distribution diagram, laying anchoring points on the mountainous area slope to obtain a mountainous area slope anchoring network. According to the invention, the prevention accuracy of freeze-thaw disasters is improved.

The invention relates to a silk fibroin biological material with high beta-fold content and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: preparing a regenerated silk fibroin solution; adding an enzyme catalytic promoter and an enzyme into the regenerated silk fibroin solution for incubation; the enzyme catalysis auxiliary agent is selected from one or more of a metal ion catalyst, a zwitterionic surfactant, a functionalized ionic liquid and a calcium phosphate nano-cluster; and centrifuging, cleaning and drying the obtained product to obtain the silk fibroin biological material with high beta-fold content. The silk fibroin material has higher beta-folding amount through primary structure modification, the space structure is more crystallized, and the amino acid sequence is more regular and ordered. The silk fibroin material provided by the invention has excellent mechanical properties, efficiency and yield, and is a biological protein material with great development potential.

The invention belongs to the field of biological materials, and particularly relates to recombinant V-type humanized collagen and application thereof. The invention provides a recombinant V-type humanized collagen, the amino acid sequence of the recombinant V-type humanized collagen comprises (repetitive unit) n, and the repetitive unit comprises a sequence as shown in SEQ ID NO.1; each repeating unit is directly linked and the number n of repeating units is 10. The recombinant V-type humanized collagen can comprehensively resist skin aging.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention relates to the technical field of synthetic biology, in particular to recombinant XVII type collagen as well as a preparation method and application thereof.The amino acid sequence of the recombinant XVII type collagen comprises a sequence shown in any one of SEQ ID NO.1-SEQ ID NO.3. The recombinant XVII type collagen can be highly expressed in an escherichia coli and pichia pastoris system, the expression system is simple, the cost is low, and the recombinant XVII type collagen is suitable for industrial production. The yield is high; the biological activity is good. The protein can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines, so that the protein can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention provides recombinant VIII type humanized collagen and application thereof, and the recombinant VIII type humanized collagen is of a triple helix structure, has good cell adhesion activity, does not generate immunological rejection and anaphylactic reaction when being applied to a human body, is a brand new human body synthetic biological material, and has good application prospects.

The TaSL1 protein or the biological material provided by the invention has the functions of improving the grain number and the ear length of wheat, and after the expression of the TaSL1 protein in wheat is reduced, the grain number, the ear length and the awn length of the obtained transgenic wheat are remarkably increased compared with those of a contrast. The TaSL1 protein or a biological material provides a new gene resource for cultivating wheat varieties with multiple grains per ear and long ears, and has important significance for improving the wheat yield.

A device and method for delivering biological material and / or synthetic chemistries to a liquid medium comprising securing a capsule to an opening of a container, the capsule comprising a cap portion for securing the capsule to the container and an elongate portion comprising a cavity defined by a frangible bottom, wherein the cavity comprises live biological material therein and a plunger comprising a disrupting mechanism, suspending the elongate portion of the capsule into an interior cavity of the container, depressing the plunger, cutting or disrupting the frangible bottom of the capsule, and releasing the material into the liquid medium.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses application of an LRK20 gene in regulation and control of rice disease resistance, a related biological material and a cultivation method. According to the application of the LRK20 gene in regulation and control of rice disease resistance, the sequence of the LRK20 gene is shown as SEQ ID NO.1, and the disease resistance is sheath blight resistance. The amino acid sequence of the protein coded by the LRK20 gene is as shown in SEQ ID NO. 2. The recombinant vector comprises the complementary sequence of the LRK20 gene in the application. The novel gene LRK20 for regulating and controlling the resistance of the rice sheath blight disease is obtained through identification, the sheath blight resistance gene LRK20 can regulate and control the resistance of the rice sheath blight disease, the resistance of the rice to the sheath blight disease can be remarkably weakened by knocking out the gene, and the resistance of the rice to the sheath blight disease can be remarkably enhanced by overexpressing the gene; therefore, the gene has a potential breeding application value in sheath blight resistance molecular breeding.

Microparticles for detecting biological materials are provided. Each of the microparticles includes: a core-shell structured microparticle consisting of a core including a magnetically responsive metal and a shell layer surrounding the core and having a uniform thickness; and capture probes introduced onto the shell layer to capture biological materials.

The invention relates to the technical field of gene isolation and plant improvement, in particular to a late blight resistance gene, a biological material and application. The Rpi-card1 nucleic acid molecule provided by the invention comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-4; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 5 or 6; the nucleotide sequence has at least 75% sequence identity with the nucleotide sequence. The invention provides an important gene resource for potato late blight resistance breeding, overcomes the problem of disease resistance loss of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.

The present invention provides a yeast recombinant type XVII humanized collagen and its preparation method, in particular, a recombinant humanized collagen expressing the 15th helical region of type XVII collagen, a recombinant engineering bacterium and its preparation method, belonging to the technical fields of genetic engineering and synthetic biology. The present invention provides the full-length sequence of the 15th helical region of type XVII collagen, and mutants are obtained by mutating amino acid residues based on this sequence; for the first time, the present invention expresses the full-length sequence of the 15th helical region of type XVII collagen in Pichia pastoris, establishes a complete set of fermentation processes and purification processes, improves the protein expression level and reduces degradation, and through experiments, it is verified that the obtained protein has biological activities such as cell adhesion activity and cell migration promotion activity that reach or exceed those of natural human collagen, can achieve the purpose of real product application, and can be widely applied in the fields of medicine, medical devices, biological materials, tissue engineering, cosmetics, etc.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

The invention discloses a pharmaceutical composition for promoting angiogenesis and tissue regeneration and application thereof. The pharmaceutical composition of the present invention comprises an acidic secretory protein rich in cysteine, a disaccharide chain proteoglycan, and an XV-type collagen alpha1 chain. The biological material system loaded with the three types of proteins can promote angiogenesis, and can improve the tissue regeneration efficiency of bone, cartilage and skin defect areas.

The invention discloses a polyphosphate kinase mutant, a biological material, a catalyst and application, and belongs to the field of protein engineering, and compared with a wild enzyme, the mutant has one or more amino acid residue mutations in the 307th site, the 311th site, the 320th site, the 408th site, the 419th site and the 431th site. The mutant disclosed by the invention is obtained by combining a semi-rational design strategy with active site region prediction and mutation library design screening, and the enzyme activity and the relative yield are remarkably improved: the efficiency of catalytic synthesis of GTP by using the preferable mutant Y431P / K311F / W408A with GMP as a substrate is 2.6 times that of a wild type enzyme, and the relative yield is improved by 150%; the efficiency of the optimized mutant Y431P / K311F / R419L for catalytically synthesizing GTP by taking GDP as a substrate is 1.9 times that of a wild enzyme, and the relative yield is increased by 82%. The key mutant obtained by screening can realize efficient synthesis from cheap NDP or NMP to high-value NTP, and has an excellent industrial application prospect.

The invention discloses an injectable material for promoting bone regeneration through piezoelectric and conductive integrated nanofiber hydrogel with ultrasonic response and a preparation method of the injectable material. The injectable material is composed of piezoelectric and conductive nanofibers and hydrogel. Wherein the piezoelectric conductive nanofiber is prepared from an inorganic piezoelectric material and a piezoelectric polymer through electrostatic spinning, or is prepared from an inorganic / organic composite piezoelectric material and a biological material with conductive performance through electrostatic spinning.

The invention belongs to the technical field of biological additive manufacturing, and provides multi-material projection photocuring biological 3D printing equipment and method.The multi-material projection photocuring biological 3D printing equipment comprises a projection mechanism, a printing platform arranged on the projection mechanism and a material supply mechanism arranged on the printing platform; the material supply mechanism comprises a plurality of charging barrels and bent pipes connected with the charging barrels, and the ends, away from the charging barrels, of the bent pipes are connected with the printing platform; the material cylinders are filled with biological materials with different properties, when the materials need to be switched, switching of material cutting and material supplying between the different material cylinders is controlled, the material switching efficiency and precision are guaranteed, and the overall printing efficiency and effect are improved.

The invention discloses a concrete crack self-repairing material based on microorganism immobilization and a preparation method of the concrete crack self-repairing material, and belongs to the crossing field of microorganism materials and civil engineering. The material is prepared from peptone, sodium lactate, volcanic rock, bacillus pasteurii and other raw materials through the steps of culture medium preparation, carrier modification, microorganism immobilization, microcapsule and gel protection layer formation and the like, the prepared volcanic rock-chitosan carrier provides an inhabiting environment for microorganisms, and the survival rate of the microorganisms in a high-alkali environment is increased to 70%. When the concrete cracks and the alkaline environment triggers the protective layer to disintegrate, the bacillus pasteurii and the desulfurization vibrio are subjected to a reduction reaction with sulfate through urealysis, and calcium carbonate and calcium sulfide are generated to fill cracks. The 28-day repair rate of the material is larger than or equal to 99%, the maximum crack repair width reaches 1.2 mm, the compressive strength recovery rate is larger than or equal to 85%, excellent durability and environment friendliness are achieved, and the concrete crack repair problem is effectively solved.

A light sheet microscope for imaging biological materials uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.

The invention discloses mutant protein of dihydroxyacetone phosphate phosphatase and application of the mutant protein. The invention provides a phosphodihydroxyacetone phosphatase mutant protein, which is characterized in that residues of phosphodihydroxyacetone phosphatase corresponding to 21st, 36th, 42nd, 49th, 64th, 82nd, 150th and 244th sites of an amino acid sequence shown in a sequence 1 are mutated to obtain a protein with phosphodihydroxyacetone phosphatase activity; and the synthesis capability of the 1, 3-dihydroxyacetone is improved. The invention also provides related biological materials and derivatives of the protein, and application of the protein in preparation of 1, 3-dihydroxyacetone. The yield of 1, 3-dihydroxy acetone of the protein is greatly increased, compared with starting wild type dihydroxy acetone phosphate phosphatase, the yield of 1, 3-dihydroxy acetone obtained through shake flask fermentation of the dihydroxy acetone phosphate phosphatase mutant SF6-11 is increased by 1.65 times, and the yield of 1, 3-dihydroxy acetone is increased to 4.54 + / -0.09 g / L from 2.75 + / -0.07 g / L.

The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.

The invention provides a GPRC5D single-domain antibody B11, and the GPRC5D single-domain antibody B11 has relatively strong binding activity and reaction specificity on GPRC5D. The invention provides an amino acid sequence of a single-domain antibody, an antibody derivative, a biological material and application thereof. The single-domain antibody provided by the invention can be used for constructing a chimeric antigen receptor T cell, and the chimeric antigen receptor T cell has stronger killing power on a target cell.

The invention provides glycosaminoglycan hydrogel with a physical and chemical double crosslinking mechanism as well as a preparation method and application thereof, and relates to the technical field of biological materials. The glycosaminoglycan hydrogel is prepared from the following raw materials: oxidized sodium hyaluronate, chondroitin sulfate, epsilon-polylysine hydrochloride, a polydopamine-titanium dioxide compound and a stabilizer. The glycosaminoglycan hydrogel is prepared through a physical and chemical double-crosslinking mechanism under the specific raw materials and proportion, the problems of cytotoxicity and the like caused by use of a crosslinking agent or introduction of other solvents are avoided, a stable covalent network is constructed through chemical crosslinking, the gel is endowed with the self-repairing characteristic through physical crosslinking, and the self-repairing performance of the hydrogel is improved. And the viscosity of the product is remarkably improved, so that the hydrogel has stronger mechanical support performance, is suitable for wound repair, wound healing and the like, and has good antibacterial activity and stability.

The invention belongs to the technical field of biological materials, and particularly relates to a preparation method of a KOH activated fungal mycelium biochar / chitosan composite material. According to the preparation method disclosed by the invention, Phanerochaete chrysosporium mycelium is used as a carbon source, and KOH chemical activation and chitosan surface modification are sequentially carried out, so that the charcoal / chitosan composite material CHKBC is prepared. The CHKBC material prepared by the method is large in specific surface area, developed in pore structure, rich in mesopores and excellent in adsorption performance and regeneration performance; the specific surface area is up to 3650-3920m < 2 > / g, the average pore size is 2-4nm, and both the specific surface area and the average pore size are higher than those of precursor biochar and activated biochar; the maximum adsorption capacity of tetracycline TC is 650-690 mg / g, which is far higher than that of KBC and BC. After five times of recycling, the average regeneration efficiency is higher than 90%, and the regeneration performance is good.

The invention relates to the technical field of the Internet of Things, and discloses a biological material component analysis method and system based on the combination of the Internet of Things and a neural network, and the method comprises the steps: obtaining material sample data of a to-be-detected biological material and environment state data of an environment where the to-be-detected biological material is located, and extracting an intrinsic attribute index of the to-be-detected biological material from the material sample data, calculating the basic substance content of the biological material to be detected; calculating the characteristic absorption rate of the to-be-detected biological material so as to analyze the characteristic absorption intensity of the to-be-detected biological material; constructing a spectral line graph corresponding to the to-be-detected biological material, and re-extracting fingerprint spectral information of the to-be-detected biological material from the spectral line graph; and inputting the fingerprint spectrum information into a biological detection unit of a preset Internet of Things, outputting a corresponding induction signal sequence by using the biological detection unit so as to identify molecular configuration information of the to-be-detected biological material, and analyzing the material composition of the to-be-detected biological material by using a trained neural network. The accuracy of biological material component analysis can be improved.

An engineered bacterium for improving tryptophan yield, a construction method therefor, and a use thereof. A strain capable of tolerating a high concentration of tryptophan was obtained by screening. Genome sequencing and protein sequence analysis of the strain revealed that some proteins in the strain had undergone point mutations, and these mutants can improve the tryptophan yield. In order to further improve the tryptophan yield, modifications are made to a protein sequence expressed by a fadR gene or a protein sequence expressed by a pepD gene in a starting bacterium. Such modifications enable the obtained engineered bacterium to have a higher tryptophan yield than the starting bacterium. In the case of large-scale production, in a 5 L fermentation tank, the tryptophan yield can reach 62.38±5.80 g / L, and the sugar-to-acid conversion rate can reach 24.1%. Compared with the original bacterium, the tryptophan yield is increased by 1.48 folds, and the sugar-to-acid conversion rate is increased by 1.26 folds. The biological material and the use thereof relate to the technical field of molecular biology and have wide practical application value.