42 results about "Merocyanine" patented technology

Cyanine and related dyes, such as merocyanine, styryl and oxonol dyes, are strongly light-absorbing and highly luminescent. Cyanine and related dyes having functional groups make them reactive with amine, hydroxy and sulfhydryl groups are covalently attached to proteins, nucleic acids, carbohydrates, sugars, cells and combinations thereof, and other biological and nonbiological materials, to make these materials fluorescent so that they can be detected. The labeled materials can then be used in assays employing excitation light sources and luminescence detectors. For example, fluorescent cyanine and related dyes can be attached to amine, hydroxy or sulfhydryl groups of avidin and to antibodies and to lectins. Thereupon, avidin labeled with cyanine type dyes can be used to quantify biotinvlated materials and antibodies conjugated with cyanine-type dyes can be used to detect and measure antigens and haptens. In addition, cyanine-conjugated lectins can be used to detect specific carbohydrate groups. Also, cyanine-conjugated fragments of DNA or RNA can be used to identify the presence of complementary nucleotide sequences in DNA or RNA.

Disclosed is a polymer film excellent in performance of inducing Rth. The polymer film comprises at least one species of a compound represented by the formula (I) and a compound represented by the formula (I′):

A subject matter of the invention is cyanine derivatives of formula:

in which the dotted lines represent the atoms necessary for the formation of one or two fused aromatic rings, each ring comprising 5 or 6 carbon atoms;

• R₁, R₂, R₃ and R₄ represent, independently of one another: H; substituted or unsubstituted C₁-C₁₅ alkyl; C₁-C₆ alkoxy; (C₂-C₁₂)dialkylamino; C₁-C₆ alkoxycarbonyl; di(C₂-C₁₂)alkylamido; a substituted or unsubstituted aryl, arylalkyl or aryloxy group; a halogen atom; a nitro; an L1-W, L2-M, L2-A or L2-G group;
• R₅ and R₆ represent, independently of one another: substituted or unsubstituted C₁-C₁₅ alkyl; a substituted or unsubstituted aryl or arylalkyl group; an L1-W, L2-M, L2-A or L2-G group;
• X is chosen from: O, S or CR₇R₈; Y is chosen from: O, S or CR₉R₁₀;
• R₇, R₈, R₉ and R₁₀ independently represent: substituted or unsubstituted C₁-C₁₅ alkyl; substituted or unsubstituted aryl, arylalkyl or aryloxy; an L1-W, L2-M, L2-A or L2-G group;
• R₇ and R₈ and/or R₉ and R₁₀ can also together form a ring comprising 5 or 6 atoms or a heterocycle comprising 4 to 5 carbon atoms and an oxygen atom;
• B represents a polymethine bridge comprising 1 to 5 methine groups, said groups being in particular individually unsubstituted or substituted by a substituted or unsubstituted C₁-C₁₅ alkyl; a substituted or unsubstituted aryl, arylalkyl or aryloxy group; a nitro group; an L1-W, L2-M, L2-A or L2-G group;
• L1 and L2 are connecting arms; G is a reactive group; A is a coupling agent; M is a conjugated molecule, W is a phosphate or phosphonate ester (preferably diester), with the proviso that the cyanine derivative comprises at least one L1-W group and at least one L2-A, L2-G or L2-M group.

This invention relates to a preparation method of high assay proto cyaniding. High assay proto cyaniding is got after solvent extraction, column chromatography separation and solvent precipitation process and original material is grape seed. Proto cyaniding content of solvent crude extract is about 50%, then it can be raised above 80% through macro pore polymeric adsorbent column chromatography refinery, and it can be raised above 90% through once solvent precipitation finally. proto cyaniding yield is above 75% of the whole process. The advantage is that technical process is simple, production cost is low, and it is convenient to industrialization.

The invention discloses procyanidine-modified carboxymethyl chitosan, a preparation method thereof, and application thereof in the fields of medical materials, health-care foods, cosmetics and the like. The procyanidine-modified carboxymethyl chitosan keeps the characteristics of carboxymethyl chitosan and has high oxidation resistance, antibacterial activity, barrier properties and the like, and is worthy of being applied.

A crosslinked product with excellent durability is provided that can remove intermediate colors of light while preventing brightness from deteriorating and thereby can improve color tone representation. The crosslinked product of the present invention includes a J aggregate of a dye and is characterized in that the dye is at least one dye selected from the group consisting of cyanine, merocyanine, squarylium, and porphyrin, the J aggregate of the dye is formed in a matrix resin, and the matrix resin has been crosslinked.

The invention discloses a method for extracting procyanidine from grape seeds by utilizing an aqueous two-phase system. The method comprises the following steps: 1) pretreating a material, namely drying grape seeds in a drying oven at the temperature of 50-80 DEG C for 18-36 hours, and grinding into powder; and 2) performing aqueous two-phase extraction, namely adding an aqueous two-phase extraction solution composed of ethanol, ammonium sulfate, distilled water and sodium chloride into a centrifugal device, adding the grape seed powder, fully mixing, centrifuging at a rotating speed of 3000-6000 revolutions per minute for 10-15 minutes, and standing for extracting, thereby obtaining procyanidine, wherein the aqueous two-phase extraction solution is composed of 30 wt%-35wt% of ethanol, 20wt%-25wt% of ammonium sulfate, 1.8 wt%-2.2wt% of sodium chloride and the balance of distilled water; and a mass ratio of the grape seed powder to the aqueous two-phase extraction solution is 1:(16-30). The method is rapid in phase separation and low in time consumption, the moisture content of the extraction system is high, an extract can be effectively prevented from being inactivated or denatured, the separation process is complete and economic and is simple in operation, and enlarged production and continuous operations are facilitated.

Described herein are chromophore compounds formed by interaction of ascorbic acid (AA) and a pyranoanthocyanin (PACN) compound. Also described herein are natural food colorant compositions that include chromophore compounds. The pyranoanthocyanins can be formed by the heterocyclic addition of the C4 and 5-OH of an anthocyanin, using a polar metabolite. Also described herein are pyranoanthocyanins, and methods of making and using the same.

The invention discloses a preparation method of a luminol-aniline copolymerization modified electrode, which comprises the following steps as follows: (a) based on ITO (indium tin oxide) glass plate as a base electrode, paving an insulating material on the conducting surface of the base electrode, forming an electrode zone at one end of the ITO glass plate, forming a connection zone at the other end of the ITO glass plate, cleaning, and drying to obtain an ITO electrode; (b) dissolving NaOH solution containing luminol and aniline in H2SO4 under stirring condition to obtain a mixed solution; and (c) carrying out electrochemical copolymerization reaction by adopting cyclic voltammetry, and taking a saturated calomel electrode as a reference electrode, a platinum wire electrode as an auxiliary electrode and the ITO electrode as a working electrode under the stirring condition in the mixed solution obtained in the step (b), cleaning and airing to obtain the luminol-aniline copolymerization modified electrode. According to the invention, the immobilization of luminescent substances is realized; and experiments prove that compared with the traditional poly luminol modified electrode, the modified electrode has the advantages that the luminous intensity is improved by about 10 times, the detection limit on procyanidin is reduced by around 4 times, and unexpected technical effects are achieved.

The invention provides a spiropyrane solid photostimulation-response compound. The molecular chemical formula of the compound is shown in a formula (I). The invention discloses a preparation method and application of the solid fluorescence stimulation-response compound containing spiropyrane and tetraphenyl ethylene. The spiropyrane is a common photostimulation-response molecule, reversible structural isomerization between the spiropyrane as a colorless closed ring body and merocyanine as a colored open ring body can be generated, and due to high molecule accumulation of the spiropyrane in a solid state, the above-mentioned isomerization cannot easily occur; the typical aggregation-induced emission phenomenon occurs on the tetraphenyl ethylene, and the tetraphenyl ethylene is large in volume and provides the free volume for the spiropyrane, so that the isomerization of the spiropyrane in the solid state is facilitated; spacers are introduced between the tetraphenyl ethylene and the spiropyrane, which is beneficial for improvement of the luminous efficiency of the spiropyrane. In this way, the solid photochromic fluorescent molecular switch material is provided and has wide application prospects in the fields of photoswitch molecular devices, anti-fake application, optical information storage and the like.

In a non-limiting embodiment, there is provided a compound of formula (I), which may permit for a method or use in treating or preventing a cancer, such as pancreatic cancer or leukemia. In one embodiment, there is also provide a method of preparing a compound of formula (Ia), the method including conducting a cyclization reaction of a compound of formula (III) to obtain a compound of formula (IV), wherein conducting the cyclization reaction comprises conducting a Michael reaction in the presence of a Lewis acid.

Anthocyanic colorant made of vegetable primary material and process for its production relate to food, cosmetic, pharmaceutical and textile industry and may be used in production of alcoholic and soft drinks, confectionery, products made of sour milk, in coloring of tablet capsules, dying of children's underwear, toys, etc. According to the invention, pelargonidin glycoside was added to the composition of the natural colorant that contained cyanidin glycosides, peonidin glycosides, organic substance and mineral salts. The components percentage shall be as follows, %: cyanidin glycoside 0.1-8.6; peonidin glycoside 0.08-6.45 pelargonidin glycoside 0.005-4.3; organic substance and mineral salts-the rest. Due to the fact that the colorant solution contains pelergonidin glycoside further to cyanidin and peonidin glycosides, the proposed colorant obtained expanded color spectrum. Combination of these three anthocyans in the proposed colorant provides for the most rich spectrum of red colors. At the same time, the ratio of pelargonidin glycoside:peonidine glycoside:cyanidin glycoside must be 1:1.5:2, respectively, and it will provide for production of a colorant with a number of valuable physical properties. Namely, the colorant retains red color when pH of the environment is not more than 7, it is thermostable, photostable and maintains its properties during 2 (two) years, besides, its relative optical density is the highest when it is subjected to the light with wavelength of 505-515 nm. According to the invention, process of the colorant production implies that pre-dried vegetable maize-pulp containing anthocyan is grinded, extracted by a mix of aqueous solutions of hydrochloric and citric acids in the field of ultrasonic vibration. Then the extracted coloring matter is filtered and concentrated in vacuum. The primary material is additionally prepared for extraction by infusion of grinded material in extracting agent during 6-8 hours at the temperature of 35-40° C. Extraction may be performed in three steps. Processing for extraction of each lot shall be 30-40 minutes at the temperature of 35-40° C. The process makes the production technology easier and provides for additional source of primary materials. 2 independent claims, 8 dependent claims, 5 tables.

The invention belongs to the field of plant effective component extraction, and provides an extraction method of cyanidin. The method comprises the following steps: (1) sample preparation; (2) leaf grinding; and (3) cyanidin extraction. The orthogonal test by using a citric acid-sodium citrate buffer solution as an extraction solvent detects that the sequence of factors responding to the purple lettuce cyanidin extraction effect is as follows: material/liquid ratio>extraction temperature>extraction time; and therefore, the material/liquid ratio is preferentially determined in the cyanidin extraction process. The control on the material/liquid ratio, extraction time, extraction temperature and extracting solution pH value proves that the method enhances the extraction rate and cyanidin leaching rate on the premise of lowering the cost and simplifying the process. Since all the extraction solvents are nontoxic and are reagents widely used as food additives, the method can satisfy the requirements of modern people for safety, and thus, has wide applicability in the fields of food, health care, drugs and the like.

The invention belongs to bis(rhodanine)merocyanine sensitizing dyes, belongs to the field of organic synthetic dyes, and especially relates to a preparation method of a bis(rhodanine)merocyanine sensitizing material by taking 2-methylbenzothiazole and a rhodanine derivative as main initial raw materials. The target product is obtained by taking low-price 2-methylbenzothiazole and rhodanine carboxylate as the raw materials and performing five-step reaction. The beneficial effects comprise that: the method is simple and easy in reaction operation, small in environmental pollution, high in product purity and relatively low in cost, and is suitable for large-scale industrial production.

The invention provides a preparation method of nitrogen-doped carbon quantum dots, and belongs to the technical field of preparation of functional materials. The preparation method comprises the following steps: mixing a crude extract containing procyanidine, an organic nitrogen doping agent and water, and carrying out hydrothermal carbonization to obtain the nitrogen-doped carbon quantum dots. The procyanidine extract is a carbon precursor, after the procyanidine extract is mixed with an organic nitrogen doping agent and then is subjected to hydrothermal reaction, the surface functional groups of the formed nitrogen-doped carbon quantum dots are abundant, and the nitrogen-doped carbon quantum dots have high fluorescence and excellent pH stability and high ion strength tolerance; and the nitrogen-doped carbon quantum dots have high selectivity on common perfluorooctane sulphonate in water. From embodiments, the detection linear range of the nitrogen-doped carbon quantum dots to the perfluorooctane sulphonate is 3-160* 10-10 mol/L, the detection limit is 0.3 nM, and the itrogen-doped carbon quantum dots can be used for rapidly detecting a trace amount of the perfluorooctane sulphonate in an environmental water sample.

The invention discloses a method for degrading high-polymeric procyanidine and belongs to the technical field of high-polymeric compound degradation. The method comprises the steps of utilizing quaternary ammonium salt type ionic liquid to conduct catalytic degradation on the high-polymeric procyanidine. When the quaternary ammonium salt type ionic liquid is utilized to conduct catalytic degradation on the high-polymeric procyanidine, the method has the advantages of being small in ionic liquid use amount, moderate in reaction conditions and high in catalytic activity. A low-polymeric procyanidine obtained through catalysis is high in content and complete in molecular structure, the quaternary ammonium salt type ionic liquid preparing process is simple, the cost is low, and the prepared quaternary ammonium salt type ionic liquid is long in storage time and is suitable for industrial large-scale production.

The invention relates to a method for extracting high-quality proanthocyanidins from pine needle. The method comprises the following steps: extracting proanthocyanidins from pine needle raw material powder by using an alkaline aqueous solvent, concentrating the obtained extract liquid, extracting, adding an anti-solvent to the obtained new extract liquid for precipitating proanthocyanidins, and filtering to obtain a highly-pure proanthocyanidin product. The method has the advantages of simplicity, energy saving, environmental protection, and no need of large apparatus investment, and is very suitable for the industrialized production. The obtained highly-pure proanthocyanidin product can be used in food antioxidants, cosmetics, healthcare foods, medicines and the like.