99results about How to "Experiment operation is simple" patented technology

The invention belongs to the technical field of kidney histopathology and immunological evaluation, and particularly relates to a kidney pathology detection kit, a detection method and application, the detection kit comprises 37 rare metal element labeled monoclonal antibodies combined with antigens of in-situ tissue cells, and the detection method comprises the following steps: (1) collecting a sample to be detected, preparing a pre-dyed paraffin section; (2) mixing 37 monoclonal antibodies labeled by rare metal elements, and carrying out antibody staining on various antigen targets on the surfaces of cells in the in-situ tissue; (3) carrying out nucleus DNA staining on the stained tissue sample; and (4) detecting the stained tissue sample through an imaging mass spectrum flow system, and collecting original information data through laser spot firing. The method is an analysis method for fine grouping identification and relative quantification of structural cells, immune cells and pathological injury cells in the clinical nephropathy sample in-situ tissue. And auxiliary diagnosis information is provided for diagnosis, grading, treatment and prognosis detection of various clinical nephropathy.

The invention relates to the technical field of disinfection effect evaluation, and discloses a method for evaluating the virus disinfection effect of virus aerosol. According to the method for evaluating the virus killing effect of the virus aerosol, a real virus is simulated by adopting a pseudovirus, so that the biological safety risk is avoided; through the innovative pseudovirus activity detection method, the experimental operation complexity is greatly reduced, the detection time is shortened, meanwhile, the detection sensitivity is improved, and the method can be applied to the disinfection effect test and evaluation of scientific research and commercial air disinfection equipment (such as ultraviolet disinfection equipment).

The invention relates to a sperm flagellum three-dimensional motion reconstruction method based on deep learning, and the method comprises the steps: obtaining a two-dimensional image of a to-be-processed sperm, carrying out the image preprocessing of the two-dimensional image of the to-be-processed sperm, and obtaining a preprocessed image; inputting the preprocessed image into a trained sperm segmentation model to obtain a sperm flagellum binary image; skeleton processing is carried out on the sperm flagellum binary image, and a sperm flagellum center line, N equidistant points and position coordinates corresponding to each equidistant point are obtained; obtaining N sperm flagellum local images based on the N equidistant points, inputting each sperm flagellum local image into a trained depth prediction model, and obtaining depth information of each sperm flagellum local image; and reconstructing a three-dimensional model of the sperm flagellum based on the depth information of each local image of the sperm flagellum and the position coordinate corresponding to each equidistant point. A reliable solution is provided for research of sperm movement behaviors and development of an assisted reproduction technology.

The invention discloses an equivalent experiment method for pressure generated by arc discharge in transformer oil, and the method comprises the steps: injecting gas into an oil tank filled with the transformer oil, and measuring the pressure in the oil tank to obtain the steady-state pressure ps in the oil tank; the temperature of the injected gas is judged, and if the injected gas is normal-temperature gas, the peak pressure pmax of equivalent arc discharge is calculated according to the gas production temperature peak value Tmax; if the temperature of the injected gas is equal to the temperature of the arc generated gas, the pressure peak value generated by the injected gas is the equivalent arc discharge peak value pressure pmax, and the injected gas is the steady-state pressure ps in the oil tank after being cooled to the room temperature; measuring the volume Vg of the injected gas; according to the relation between the gas volume and the arc energy, equivalent arc discharge energy Earc is calculated; and comparing the pressure waveform generated by discharge when the arc discharge energy is Earc with the pressure waveform of the equivalent experiment, and verifying the feasibility of the equivalent method.

The invention belongs to the technical field of micro-fluidic chips, and discloses a hepatic lobule chip which comprises a single cell capture array chip, a micropore array chip and an antibody slide, a capture array on the single cell capture array chip and a micropore array on the micropore array chip are in a polygon shape, perfusion culture solution channels are arranged in the diagonal directions of all vertexes of the polygon, and the capture array and the micropore array are in one-to-one correspondence after being assembled. The liver lobule chip overcomes the dependence of research on spatial heterogeneity of liver cells on precise liver anatomical slices, avoids the defects that the liver slices are difficult to obtain and store, can directly reveal spatial distribution characteristics of secretion activity of the liver cells in the liver lobule, and becomes an ideal tool for spatial heterogeneity secretion of the liver cells. The invention further discloses application of the hepatic lobule chip in hepatic lobule spatial heterogeneity single cell secretion analysis, operation is easy, large experimental instruments are not needed, cost is low, and the hepatic lobule chip is easy to master.

The invention relates to the technical field of plant genetic engineering, and provides an agrobacterium rhizogenes-mediated sunflower transient transformation system and a construction method thereof, sterile conditions and tissue culture are not needed, a vector to be transformed is transferred into agrobacterium rhizogenes, positive strains are cultured and screened, and a dip dyeing solution is prepared; cutting a stem section of the sunflower, and pricking holes near a cut; immersing the stem segments in the dip dyeing liquid for a period of time; taking out and inserting into sterilized vermiculite soil mixed with orobanche coerulescens for rooting to obtain an instantly transformed sunflower plant; the method is easy and convenient to operate and short in period, the expression level of the HaCERK1 gene can be effectively improved, the resistance of sunflowers to orobanche coerulescens infection is enhanced, and the gene function research period is shortened.

The invention relates to a rigid spiro compound and a preparation method and application thereof.The preparation method comprises the steps that 6-methylene-androstane-4-ene-3, 17-diketone (V) is dissolved in a mixed solution of dichloromethane and trifluoroacetic acid, reaction and reduced pressure distillation are conducted at the temperature of 20-25 DEG C, then extraction washing and distillation are conducted, a rigid spiro compound crude product can be obtained, and the rigid spiro compound is obtained. The crude product can be subjected to column chromatography separation and purification to obtain a refined rigid spiro compound (I). By adopting the preparation method, the friction and wear properties of the base oil are remarkably improved, and the base oil has lower friction coefficient and wear scar diameter, and has good application prospect as an antifriction and wear-resistant additive.

The invention relates to the technical field of nano material preparation, in particular to a zinc ion battery cathode material as well as a preparation method and application thereof. The zinc ion battery cathode material provided by the invention has the advantages of uniform shape, uniform size, high first cycle capacity and coulombic efficiency, and good cycle performance. According to the preparation method of the zinc ion battery cathode material provided by the invention, the bimetallic metal organic framework heterogeneous nanosheet material with different doping contents can be prepared by adjusting the concentration of the transition metal ions, and the activity and stability of the material are changed by changing the variety of the transition metal ions. The preparation method disclosed by the invention is also beneficial to large-scale preparation. The method does not need complex equipment, the experimental operation is simple, and macro preparation is easy. The zinc ion battery cathode material provided by the invention has important application in the aspect of electrochemical energy storage, and is helpful for improving the electrochemical performance of various types of battery electrodes.

The invention discloses a visual flexible deformation expansion section test device for a two-dimensional rectangular spray pipe. The visual flexible deformation expansion section test device comprises a spray pipe test section, a transition section, a connection section, a rectification section and an air inlet section which are communicated in sequence, the spray pipe test section comprises a spray pipe mounting frame and a spray pipe main body part, the spray pipe main body part is formed by connecting and enclosing an upper molded panel, a lower molded panel, a left optical glass plate and a right optical glass plate, a glass baffle is arranged on the outer side of the optical glass plate and abuts against the optical glass plate, and the glass baffle is provided with a penetrating opening serving as an observation window; a pressure measuring connector is mounted on the upper molding panel; the lower profile panel is provided with an elastic wall plate mounting port and an elastic wall plate, the lower side surface of the lower profile panel is provided with a cover plate, a cavity is formed between the cover plate and the elastic wall plate, and the cover plate is provided with an air guide hole communicated with the cavity; and a pressure measuring connector, a total pressure measuring device and a temperature measuring connector are mounted on the connecting section. The influence of wall surface ablation movement and vibration of the expansion section on an internal flow field of the spray pipe in the working process of the spray pipe can be observed and recorded.

The invention relates to the field of biological analysis and detection, in particular to a method for super-resolution imaging of bacterial extracellular newborn protein. On the basis of biological orthogonal amino acid and alkyne fluorescent dye, super-resolution imaging in the in-situ visualized space-time deposition process of the extracellular newborn protein is realized by utilizing a super-resolution microscope. Compared with a traditional research method, the method has the advantages of being easy to operate, rapid, efficient, high in universality and low in biological disturbance, the imaging challenge of space-time deposition of the extracellular matrix is solved, key information related to the extracellular matrix is provided, understanding of the biological membrane forming process is deepened, and good application prospects are achieved.

The invention belongs to the technical field of three-dimensional ceramic network reinforced composite phase-change materials, and particularly relates to a high-thermal-conductivity and insulating three-dimensional ceramic network reinforced composite phase-change material and a preparation method thereof.The high-thermal-conductivity and insulating three-dimensional ceramic network reinforced composite phase-change material comprises an organic solid-liquid phase-change material serving as a working component, an inorganic solid-liquid phase-change material serving as a working component and an inorganic solid-liquid phase-change material serving as a working component, the polyhydric alcohols are selected from paraffin, polyethylene glycol, stearic acid and polyhydric alcohols; an encapsulating component: a steady-state polymer material selected from epoxy resin and polyurethane; the reinforcing filler is a high-thermal-conductivity insulating inorganic filler and is selected from boron nitride, aluminum oxide and silicon nitride.The preparation method has the advantages that compared with a traditional porous ceramic preparation process, controllable preparation of the high-thermal-conductivity insulating porous ceramic with a complex structure is achieved by adopting a 3D printing technology, and designability and repeatability of the structure of the high-thermal-conductivity insulating porous ceramic can be achieved easily.

The invention discloses a preparation method and application of an i-motif-containing DNA surface modified carbon quantum dot, and the carbon dot is prepared by the following steps: synthesizing a carbon dot, and then carrying out DNA modification. The preparation method comprises the following steps: dissolving phenylenediamine in a polymer solution, uniformly mixing, transferring the solution into a polytetrafluoroethylene inner container, putting the inner container into a stainless steel high-pressure kettle, and heating and reacting in a muffle furnace. And after the reaction is finished, cooling a reaction system to room temperature, then polymerizing DNA containing i-motif as a cross-linking agent with CDs, after the polymerization reaction is finished, freeze-drying the solution, taking out the solution, putting the solution into a dialysis bag, and carrying out dialysis purification to obtain DNA-CDs after dialysis is finished. And then carrying out bionic treatment on the MV-DNA-CDs, so as to obtain the MV-DNA-CDs disclosed by the invention. The nano-carrier prepared by the invention is high in yield and good in stability, has responsiveness and targeting property, and can be used for precise treatment of tumors.

The invention relates to the technical field of surface and interface modification of a battery positive electrode material, and discloses a surface and interface modification method of a Prussian blue positive electrode material, which comprises the following steps: mixing sodium citrate and FeSO4. 7H2O in deionized water to prepare a 100mL solution marked as a solution a; the mass ratio of the sodium citrate to the FeSO4. 7H2O is (6-8): (1-3); dissolving sodium citrate and Na4Fe (CN) 6. 10H2O in deionized water to form a 100mL solution, and marking the 100mL solution as a solution b; the mass ratio of the sodium citrate to the Na4Fe (CN) 6. 10H2O is (6-8): (1-2.5). The material is prepared by adopting a coprecipitation method, modification of the surface interface of the Prussian blue positive electrode material is realized by directly utilizing metal titanium ions, and the method is simple and efficient in experimental operation and mild in condition, and has a relatively wide application prospect in actual industrial application.

The invention discloses application of a dual hydrocarbon activation strategy to synthesis of novel non-natural amino acids and polypeptides, belonging to the technical field of organic synthesis. The method comprises the following steps: by taking a lysine derivative and acrolein as starting substrates, reacting for 2 hours at the temperature of 40 DEG C under the action of a rhodium catalyst to obtain a series of non-natural amino acids and polypeptides. The method has the advantages that reaction conditions are mild, raw materials are easy to prepare, only one-step reaction is needed, and experimental operation is simple; antibacterial experiments show that the novel non-natural amino acids and polypeptides have good antibacterial activity on fungi in agriculture and forestry. # imgabs0 #

The invention discloses a cell culture medium additive as well as a preparation method and application thereof. The cell culture medium additive comprises the following components: beta-sitosterol, sericin, hydrolyzed corn starch, vitamins and a liquorice extract. Cortical neurons cultured by the cell culture medium additive provided by the invention are high in survival rate and good in growth state, and the purity reaches 90% or above; all the components in the cell culture medium additive provided by the invention have a synergistic effect, so that the cell culture medium additive plays a role in promoting the survival, growth and development of neurons and can prevent the neurons from being damaged to die; meanwhile, the antibacterial effect can be achieved without adding antibiotics, and adverse effects of the antibiotics on growth and differentiation of nerve cells are avoided; when the cell culture fluid provided by the invention is used for culturing cells, the experimental operation can be simplified, the cost can be reduced, and the stability and repeatability of experimental results can be improved.

The invention relates to the technical field of water treatment resin regeneration, in particular to a water treatment ion exchange resin separation degree testing device and method.The water treatment ion exchange resin separation degree testing device comprises a separation column, a sand core overflow opening is formed in the separation column, and a piston valve is arranged at the bottom end of the separation column; a sand core drain valve is arranged at the bottom end of the resin measurer, a sand core emptying valve is arranged on the resin measurer, and a grease discharging valve is arranged on the outer wall of the end, close to the sand core drain valve, of the resin measurer; the bottom end of the separation column is connected with the top end of the resin measurer through a piston valve, the experimental operation for testing the resin separation degree by using the device is rapid and simple, meanwhile, the resin volume can be accurately measured under the condition that the sample amount is small, the testing precision of the resin separation degree is guaranteed, and the testing efficiency is improved. And a basis is provided for accurately judging the operation condition of the condensate polishing regeneration system.

The invention relates to a method for catalytically synthesizing 2, 4-disubstituted phthalazine 1-(2H)-ketone from 1, 5, 7-triazabicyclo [4.4. 0] dec-5-ene. According to the method, 3-alkenyl phthalide and hydrazine compounds are taken as initial raw materials, TBD which is low in price and easy to obtain is taken as a catalyst, sodium tert-butoxide is taken as alkali, reaction is carried out at room temperature, and the 2, 4-disubstituted phthalazine 1-(2H)-ketone is obtained at high yield. According to the method, the cheap TBD is adopted as the catalyst, the reaction is carried out under the room temperature condition, and compared with a traditional reaction strategy, the economic cost is greatly reduced, the steps are simple, heating and a transition metal catalyst are not needed, operation is convenient, the yield is high, the cost is low, and good economic benefits are achieved.

The application discloses a synthesis method of C3-substituted quinoxaline ketone derivatives and application thereof. A series of C3-substituted quinoxaline ketone derivatives with both terpene and quinoxaline ketone biological activity skeletons are obtained by using N-alkoxy phthalimide and quinoxaline ketone as starting substrates, under the catalysis of a photocatalyst and visible light irradiation, and at a temperature of 55-65 DEG C for 10-15 h. The application has the advantages of stable and easy-to-prepare raw materials, mild reaction conditions, simple experimental operation, good chemical selectivity and functional group tolerance, and is a green chemical synthesis method with good application prospect. The activity experiment results show that the synthesized C3-substituted quinoxaline ketone derivatives have good inhibiting effect on the growth of plant pathogenic fungi.

The utility model discloses a kind of multifunctional lithium battery thermal dynamics experimental device, belong to lithium battery thermal dynamics technical field, the device includes box, fixed frame is placed inside box, fixed frame includes fixed plate, fixed groove is equipped on fixed plate, heating assembly is placed in fixed groove, battery compartment for placing multiple lithium batteries is equipped in heating assembly, the top of heating assembly is connected with protection plate;The top and bottom of heating assembly are equipped with multiple power supply sockets and detection socket, multiple power supply columns and temperature sensing probe are separately equipped in protection plate interior, fixed groove top;Multiple grooves are sequentially equipped on the both sides of fixed groove, rotatable electrode plate is equipped in groove, the inside of electrode plate is equipped with contact block, rotate multiple electrode plate and heat assembly is clamped between multiple electrode plate, and the contact block of electrode plate is all contacted with lithium battery in heating assembly.The utility model can accurately measure multiple data, provide reliable data support for lithium battery thermal dynamics research.

The invention discloses a dwarf sperm vitrification ultralow temperature cryopreservation method, and relates to the technical field of marine organisms. The preservation method comprises the following steps: (1) taking dwarf at a breeding period, sequentially drying in the shade and stimulating at variable temperature to naturally discharge sperms, filtering and centrifuging to obtain high-density sperms, and preserving the sperms on ice; (2) mixing the high-density sperms obtained in the step (1) with a cryoprotectant, and placing the mixture on ice to obtain a mixed sperm solution; the freezing protection liquid is prepared from the following components: dimethyl sulfoxide, coenzyme Q10 and polysucrose; and (3) transferring the mixed sperm solution into a freezing tube, and directly immersing the freezing tube into liquid nitrogen for preservation. Research finds that after the sample is unfrozen for 60-64s at the temperature of 50-60 DEG C, the rate of D-shaped larvae reaches 80% or above, and the freezing method that the sample is directly put into liquid nitrogen for long-term preservation is achieved.

The invention discloses a universal molecular beacon kit for various animal-derived components, and belongs to the technical field of molecular biological detection. Aiming at gene segments with specific structural characteristics in 11 common livestock and poultry animal genomes, the invention designs a composition of a universal primer and a molecular beacon fluorescent probe. The combination can be used for carrying out real-time fluorescent PCR detection and melting curve analysis by using the kit and the method by taking genome DNA of a sample to be detected as a template. By comparing the shape of a melting peak of a detected sample and the characteristic melting temperature (Tm value) of the detected sample, 11 animal-derived components of livestock and poultry can be quickly and accurately identified under the condition of single-tube detection. Technical innovation is achieved in the field of molecular biological detection, an efficient and reliable solution is provided for animal-derived component detection, and the method has important practical value and popularization potential.

The utility model relates to experimental apparatus technical field of ross foam appearance, concretely is a kind of ross foam appearance automation measuring device based on light intensity detection, including conventional ross foam appearance and its both end support, 15 groups wide-angle infrared opposite transmission sensor are arranged in 40mm vertical interval along support outer wall, each group of sensor contains infrared emission tube and receiving tube, arc clamp and its bolt hole and sensor recess for fixing sensor;Signal processing unit, connect sensor for converting light intensity signal into foam height data;Display unit, connect signal processing unit for real-time output measurement result;Power module, power supply for each unit.The utility model on the basis of original ross method operating procedure, through the light transmittance variation of real-time monitoring at different heights by multiple infrared opposite transmission sensors, realized the automation measurement without manual intervention, effectively avoided manual reading error and foam wall hanging interference, improved measurement accuracy and repeatability, simplified experimental operation.

The invention discloses a D-protein large-scale screening method based on a thermal proteomics technology, which comprises the following steps: step 1, respectively adding a protein treating agent or not adding the protein treating agent into cells to obtain a D-protein up-regulation group and a D-protein blank group; step 2, obtaining a thermal stability differential protein list by using a thermal proteomics technology; step 3, acquiring a stereoisomeric protein list by utilizing a heterogeneous retrieval technology; step 4, comparing and analyzing the heat stability differential protein list and the stereoisomeric protein list to obtain potential D-protein; and step 5, based on an APM enzyme digestion technology, obtaining the D site of the potential D-protein. The large-scale identification method of the D-protein with high throughput and high coverage is realized through a thermal proteomics technology, the D-amino acid site can be accurately positioned, and the method has important significance on exploration of disease biomarkers, exploration of pathogenesis of major diseases and the like.

The application discloses a preparation method of 2-cyano-3-hydroxy-2-buten-(4-trifluoromethyl-phenyl)amide, which comprises the following steps: reacting a compound of formula (II) with acetyl chloride or acetic anhydride under the action of a base; wherein the base comprises sodium ethoxide and / or sodium methoxide. In the application, sodium hydride with a relatively high risk coefficient is replaced by sodium alcoholate, the operation difficulty is reduced, the yield is improved compared with sodium hydride, and the application is more suitable for industrial production.

The invention relates to molecular markers of wheat grain development genes TaEND1 and KASP and application of the molecular markers. According to the invention, a new gene TaEND1 related to wheat grain development is excavated, and time and cost are saved in breeding and breeding by developing molecular markers. The KASP molecular marker is developed for the wheat grain development defect gene TaEND1, so that the genotyping efficiency, accuracy and economical efficiency are improved, and the KASP molecular marker has remarkable advantages in operation simplicity and convenience and result acquisition speed. Compared with the prior art, the KASP technology is more suitable for large-scale screening and marker-assisted selective breeding, and is helpful for accelerating the research progress of wheat grain development genes and the development of new varieties. Due to the beneficial effects, the KASP technology becomes an indispensable tool in modern wheat breeding.

The invention relates to the technical field of gene detection, in particular to a hemophilia virulence gene variation type detection method, which comprises the following steps: comparing sequence reading of third-generation long-reading long sequencing with a reference genome, comparing a part of sequence reading to an F9 gene region and a VWF gene region to obtain a first comparison result, and comparing the first comparison result with a second comparison result to obtain a second comparison result; performing first variation analysis according to the first comparison result, outputting a first analysis result, performing haplotype typing on the F8 gene region to obtain a differential base sequence of an unconventional gene and a highly homologous sequence of the unconventional gene, and comparing the differential base sequence with the residual sequence reading to obtain a second comparison result; and performing second variation analysis according to the second comparison result, and outputting a second analysis result. According to the application, multiple variation types of the hemophilia virulence gene can be screened at the same time, and variation analysis of the F8 gene with homologous gene sequence interference can be solved.

The invention relates to a dendronized phenylacetylene cross-linking agent as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out amidation reaction on dendronized active ester and amino alcohol to generate an intermediate product; and carrying out esterification reaction on the intermediate product, dialkynylbenzoic acid and a catalyst to generate the dendronized phenylacetylene cross-linking agent. The dendronized phenylacetylene monomer and the dendronized phenylacetylene cross-linking agent are used as main bodies, the good water solubility and temperature sensitivity of the cross-linking agent are utilized, temperature-sensitive aggregation of the monomer can be achieved under the condition that no additive exists, then in-situ cross-linking polymerization is conducted, and the uniformly-dispersed dendronized spiral polyphenylacetylene micro-nano gel is obtained. Good biocompatibility is shown, and potential application value can be achieved in the fields of biosensing, chiral drug delivery, biological tissue engineering and the like.

The invention discloses an automatic compensation method for a three-axis magnetic field of an atomic spin inertial measurement device. The method can quickly and accurately compensate the three-axis magnetic field. According to the method, compensation of a Z-direction magnetic field is realized by measuring a three-axis residual magnetic field through a dichotomy, and simultaneous compensation of an X-direction magnetic field and a Y-direction magnetic field is realized through a non-orthogonal decoupling measurement method. The method is reasonable, the experimental operation is simple, the method is suitable for an atomic spin inertial measurement device based on an alkali metal electron spin and inert gas nuclear spin coupling ensemble, the three-axis magnetic field can be rapidly, accurately and automatically compensated in real time, a basis is provided for development of a high-precision atomic spin inertial measurement device, and the workload of device debugging is greatly reduced.

The invention belongs to the technical field of friction experiment equipment, and relates to a device and a method for testing friction of a prepreg interface at any fiber laying angle. The device comprises a first clamping assembly used for fixing a first prepreg layer, a second clamping assembly used for fixing a second prepreg layer and a third material clamping assembly used for clamping a third prepreg layer, and the third material clamping assembly is clamped between the first clamping assembly and the second clamping assembly. The material clamping assembly comprises a material clamping part and a connecting part; the material clamping part comprises a material winding body and a pressing plate, and the material winding body is wrapped with the third prepreg layer; the material winding body comprises a middle clamping plate and a material winding block, and the middle clamping plate and the connecting part are located between the two pressing plates; the material winding block penetrates through the two pressing plates and the connecting part, and the two sides of the material winding block protrude out of the two pressing plates. The material winding block is in a regular hexagon shape, and the third prepreg layer is laid on any two opposite sides. The problems of tedious operation and low test efficiency in the prior art are solved.

This utility model discloses a base lens holder, including a clamping rod, a fixed base, a locking plate, and a positioning device connector. One end of the clamping rod is threadedly connected to the fixed base. The locking plate is threadedly connected to the fixed base and is used to change the tightness of the holder. The fixed base is an integral structure, including a first clamping arm and a second clamping arm. The first clamping arm includes a first clamping tip, and the second clamping arm includes a second clamping tip. There is a millimeter gap between the first and second clamping arms, and the first and second clamping tips form a non-closed clamping groove. After the object to be clamped is placed in the clamping groove, it is locked by the locking plate. The clamping groove can be clamped on both sides; the upper part can clamp a microscope, and the lower part can clamp a base lens, making the connection process between the base lens and the microscope very convenient and quick.