1119results about How to "Experiment operation is simple" patented technology

The invention discloses a making method of high-hydrophilicity alumina film, which comprises the following steps: adopting electrochemical polished aluminium foil as anode and platinum piece as cathode; selecting one of sulfuric acid, oxalic acid phosphoric acid with water or poor water (the composite solution of oxalic alcohol and water) as electrolyte solution; using electrochemical oxidizing method to strip aluminium base; removing damping layer; obtaining the product with different shapes and areas.

The invention relates to a partial-compensation aspherical reflector surface shape detection method, which comprises the following steps of demarcating partial-compensation elements to obtain demarcated phase distribution Wb; using the demarcated phase distribution Wb, sphere theory wavefront Wq and aspherical theory wavefront Wf to obtain phase distribution Wy of digital virtual sample plates; measuring an aspherical reflector surface to be measured to obtain phase distribution Wa; and reducing the phase distribution Wy from the measured phase distribution Wa to achieve surface shape error distribution Wm of the aspherical surface. The partial-compensation aspherical reflector surface shape detection method can accurately measure optical aspherical surface shapes by using a single partial-compensation element, thereby reducing design difficulty of a compensator, simplifying structure of the compensator and being clear in physical definition, simple in data processing and arithmetical operation, simple and practical in experimental operation, low in detection cost and short in test time.

The invention provides a method for detecting the character of pores of pervious concrete by processing images. The method comprises the following steps: cutting a pervious concrete sample transversely to expose an intact and smooth cutting plane which serves as an experimental surface; obtaining the images of the obverse side of the experimental surface of the brick-powder processed pervious concrete; selecting the largest regular section image of the sample from the obtained images of the obverse side, and calculating the total pixel value of the largest regular section image; blacking and whitening the selected image; extracting the pore image of the blacked and whitened image, and calculating the pixel value of each pore; calculating the total pixel value of the pores; calculating the total porosity and the distribution situation of internal pore diameters according to the obtained pixel values of the pores and the obtained total pixel value of the pores; repeating the steps, obtaining the total porosity of a plurality of cutting surfaces of the same pervious concrete sample and the distribution situation of pore diameters, and calculating the average value of the total porosity of the cutting surfaces of the same pervious concrete sample to obtain the total porosity of the pervious concrete sample and further the character of the pores of the pervious concrete.

The invention relates to a method for detecting a nonzero digit compensation light-degree optical non-spherical profile. The method comprises the following steps of: calculating the value of z(x, y)-s(x, y, r), wherein z(x, y) is the rise distribution of an aspheric surface along the direction of an optical axis, and s(x, y, r) is the rise distribution of a spherical surface closest to the aspheric surface to be detected along the direction of the optical axis; converting emergent parallel light of an interferometer into standard spherical waves by utilizing a transmission ball and using the standard spherical waves as reference spherical waves; adjusting the position of the aspheric surface to be detected to ensure that the circle center of the spherical surface closest to the aspheric surface to be detected coincides with a focus point of converging the reference spherical waves; detecting phase distribution data of interference fringes formed by the optical waves reflected by the aspheric surface to be detected and the reference spherical waves by utilizing the interferometer; and rejecting the value of the z(x, y)-s(x, y, r) and an adjusting and positioning error epsilon (x, y) from the phase distribution data of the interference fringes to obtain the profile error distribution e(x, y) of the aspheric surface. The invention can realize the detection of large-caliber concave and convex light-degree aspheric surface profiles with high resolution and high precision and has the advantages of low detection cost and short detection cost.

The invention relates to a method for detecting surface figures of a large-aperture off-axis convex aspheric mirror. The method comprises the following steps of: performing null interferometry on reference sub-aperture regions by using computer-generated holographic elements which correspond to surface figures of the reference sub-aperture regions of an aspheric mirror to be detected; performing null interferometry on off-axis sub-aperture regions by using computer-generated holographic elements which correspond to surface figures of the off-axis sub-aperture regions of the aspheric mirror to be detected: solving the distribution of full-aperture surface figures of the convex aspheric mirror to be detected; converting coordinates of all off-axis sub-apertures in an x-y-z coordinate system into coordinates below an X-Y-Z coordinate system and obtaining the distribution of full-aperture phases of the convex aspheric mirror to be detected by calculation; and removing system adjustment errors from the distribution of the full-aperture phases to obtain the error distribution of the full-aperture surface figures of the convex aspheric mirror to be detected. The invention has the advantages of simple data processing and mathematical operation, easy experiment operation, low detection cost and short testing time.

The invention discloses a chlorantraniliprole preparation method. A technical scheme adopted by the invention is characterized in that the chlorantraniliprole preparation method comprises the follow synthetic steps: 1, 2-amino-3-methyl-5-chlorobenzoate synthesis; 2, 3-bromo-1-(3-chloropyridine-2-pyridyl)-1H-pyrazole-5-formic acid synthesis; and 3, chlorantraniliprole synthesis. The invention concretely relates to synthetic methods of the key chlorantraniliprole intermediates comprising 2-amino-3-methyl-5-chlorobenzoate and 3-bromo-1-(3-chloropyridine-2-pyridyl)-1H-pyrazole-5-formic acid. The synthetic method of the pesticide chlorantraniliprole has the advantages of low cost, high yield, strong operability, realization of the industrialized production, and the like.

The invention relates to a PCR primer for a human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on a NGS technology and application thereof. The PCR primer for the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence comprises at least one capturing primer pair, and sequences of a forward primer and a reverse primer of each capturing primer pair respectively comprise a specific sequence and a linker sequence connected with a terminal 5' of the specific sequence. The PCR primer used for amplifying the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on the NGS technology and application thereof have the advantages that experiment operations related in the whole method are simple, only a PCR reagent and a primer combination are related, cost is low, and a ditag sequencing linker sequence is introduced for distinguishing different samples, so that high throughput sample sequencing detection can be realized, and gene detection support can be effectively provided for risk assessment on human breast cancer and other BRCA susceptible gene-related hereditary tumours or targeted medication specific to BRCA mutation.

The invention discloses a preparation method and application of self-floating high-efficiency photothermal conversion seawater desalination material, and belongs to seawater desalination materials. Ablack body absorption material with a high-efficiency photothermal conversion function and a composite nano structure grows in a three-dimensional network structure by using a chemical method, such asnickel black, eriochrome black, transition metal iron / defective iron oxide, copper / defective copper oxide compound and cobalt / defective cobalt oxide compound. Base foamed nickel in the high-efficiency photothermal conversion seawater desalination material has a hydrophobic property and good mechanical strength, and the capillary action of mutually connected pore structures of the base foamed nickel is used for continuously transporting moisture and reducing the evaporation potential barrier; the photothermal material has a wide spectral absorption function, and has self-floating performance and low thermal conductivity simultaneously, so that the generation of local high temperature is facilitated, the evaporation of water is promoted, a large loss of thermal energy is prevented, and the generation efficiency of water vapor is high.

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention relates to beaded electrospun nanofiber immobilized saccharomycete and a preparation method thereof. Nanofiber comprises electrospun polymer or macromolecules, a solvent and a saccharomycete; prepared mixed solution is placed in an injector and is spun with an electrospinning instrument, microorganisms are introduced into the nanofiber by dispersion of the saccharomycete in a spinning solution, the advantages of the nanofiber such as high specific area, high porosity, large length-diameter ratio and bulkiness and porousness are combined, in-situ immobilization is achieved for saccharomycete cells, an active organic / microorganism composite is obtained, and a function similar to a multi-enzyme bioreactor is enabled. A product and preparation method of the invention have the advantages such as high processing efficiency, high reacting speed, ease of control, high antifouling capacity, continuous reusability, and ease of product separation. The beaded electrospun nanofiber immobilized saccharomycete is applicable to the fields such as biological catalysis, filtering and adsorption, drug delivery and tissue engineering.

The invention discloses a molecular marker auxiliary selection method for TYLCV breeding, and belongs to the technical field of vegetable disease resistance breeding. The method comprises the following steps: (1) extracting DNA from breeding separation generation materials; (2) sieving SCAR molecular markers of anti-TYLCVD genes; (3) performing double PCR amplification and electrophoretic analytic detection on the DNA; and (4) evaluating the resistance of the breeding separation generation materials according to the detection result. The method can accurately and quickly detect whether any generation material in the breeding process is polymerized with the anti-TYLCV genes in a laboratory so as to obviously shorten the period of disease resistance breeding and reduce the breeding scale. Moreover, the method has the advantages of simple and convenient operation, time conservation, labor conservation, cost reduction and the like, reduces the workload, improves the sieving efficiency of resistant materials, accelerates the disease resistance breeding process, and can be popularized and applied to tomato breeding units.

The invention relates to a preparation method and application of a large size and shape ratio graphene microsheet. The method comprises the following steps: arranging oxidized graphene paper or a mixed material containing partial impurities in a quartz tube, introducing H2 at a certain air pressure, and focusing a sample for performing scanning heating by using high-speed laser, wherein a complete graphene microsheet with less than ten atomic layers and high electric conductivity and high uniformity can be prepared in a room temperature environment under certain conditions, and the impurities in the material can be finely processed. The average atomic layer number of the prepared graphene is 6-8, the particle size is between 2.0 and 3.0nm, and the shape ratio of the microsheet can be close to 10,000. The optical and conductive performances are obviously superior to those of the graphene microsheet prepared by the traditional chemical reducing method; according to fine regulation of the laser scanning parameters, the method has the advantages of repeatedly performing sample treatment and pattern processing, along with high target processability, controllable layer number and size of the microsheet, simple equipment, cleanliness, high efficiency and flexible operation, and has excellent industrial application value.

The invention provides a transition metal chalcogenide nanosheet material and a preparation method thereof, a battery anode material, a secondary battery and an application thereof, and belongs to thetechnical field of transition metal chalcogenides. The invention provides a preparation method of a transition metal chalcogenide nanosheet material, comprising the following steps: dissolving raw materials in an aqueous solution of soluble salt, removing moisture to obtain a solid, sintering the solid, and washing to obtain a transition metal chalcogenide nanosheet material, wherein the raw materials include a chalcogen precursor and a transition metal precursor. According to the preparation method, the soluble salt is used as a template, is inexpensive, is environmentally friendly and is easy to remove in post-treatment, and the obtained transition metal chalcogenide nanosheet material has a thinner sheet thickness. Compared with a traditional silica template method or a hydrothermal method, the preparation method of the invention greatly shortens the synthesis cycle, is simple and feasible, is controllable, has characteristics of high yield and simple experimental operation, and issuitable for large-scale production.

The invention discloses a method for quantitatively detecting telomerase activity based on a nano pore channel and electrochemical sensing.The method comprises the following steps that firstly, a telomerase recognition sequence is connected to the inner wall of a porous anodic alumina template or a polyethylene terephthalate film to serve as a primer; secondly, telomerase amplifies the primer to form a G-rich sequence; a G-quadruplex is formed in the presence of potassium ions; electrochemical detection is conducted on the current produced through indicating molecules of the nano pore channel by utilizing an electrochemical workstation.The method does not need complex material preparation and DNA probe marking, and can avoid the defects of high detection cost, complicated operation and poor reproducibility caused by the complex material preparation and DNA probe marking and has the advantages of being low in cost, quick, simple, convenient, high in sensitivity and good in accuracy.

The invention discloses a hydrophilic and oleophobic easy-to-clean organic coating and a preparation method thereof. The organic coating is obtained by curing an organic solution by a curing agent, the organic solution is a fluorine-containing polyurethane modified epoxy resin solution formed by carrying out a reaction on fluorine-containing polyurethane and an epoxy resin in an organic solvent, and the fluorine-containing polyurethane and the epoxy resin form a semi-interpenetrating network structure formed through the bonding of chemical bonds. A micro area with uniformly distributed hydrophilicity and oleophobicity is constructed on the surface of an object to be coated through the semi-interpenetrating structure and low surface performances of fluorine, so the hydrophilic and oleophobic easy-to-clean organic coating is formed. Experiments prove that the lowest contact angle between the organic coating and water can reach 15DEG, and the highest contact angle between the organic coating and oil can reach 90DEG, so water can thoroughly displace oil stains on the surface of an original solid, and an easy-to-clean effect is reached.

The invention discloses a high-temperature high-pressure glass micro model gripper used for heavy oil thermal recovery. The high-temperature high-pressure glass micro model gripper comprises a high-temperature high-pressure bin and high-pressure glass visual windows and further comprises a drawer type micro model support, upper pressing plates and lower pressing plates. The high-temperature high-pressure bin is a square-cylinder-shaped bin body. The upper portion and the lower portion of the inner wall of the bin body are each provided with an annular step. One high-pressure glass visual window is fixed to each annular step. The upper pressing plates and the lower pressing plates are arranged on the outsides of the high-pressure glass visual windows correspondingly. The high-temperature high-pressure bin is provided with a heater leading to an inner cavity of the bin body. The side face of the bin body is further provided with a model support inlet. The drawer type micro model support is pushed into the bin body from the model support inlet. Through the drawer type high-temperature high-pressure gripper, operation is simple, fast and convenient, and the problem of the unreliability of the leakproofness of the bin body due to repeated disassembly and assembly of the visual windows is avoided. A plurality of injection openings and control valves, bull plugs and back-pressure valves of the injection openings are adopted, so that noninterference between injection fluid is achieved, and meanwhile, the continuity of the injection fluid and pressure is ensured.

The invention relates to the technical field of electric power, in particular to a system and method for testing the electric conductivity of a metal wire. The system comprises a direct-current power supply, a wire clamping system and a test system. The output end of the direct-current power supply is in series connection with the metal wire to be tested through a fixture. The metal wire to be tested is connected to an optical platform through a fixture. A first camera and a second camera are respectively and electrically connected with a computer. A multiple-pass digital multimeter V is connected with the metal wire to be tested and is further electrically connected with the computer. A multiple-pass digital multimeter A is connected with the metal wire to be tested through the direct-current power supply. Due to the adoption of the system and method for testing the electric conductivity of the metal wire, the electric conductivity of a metal wire of any length and section shape can be tested without considering the effect of the temperature on the resistance of the wire to be tested, and experiments are easy and quick to operate. The system and method greatly improve working efficiency, obviously improve testing accuracy and are suitable for all kinds of novel energy-saving metal wires.

The invention provides an HLA (Human Leukocyte Antigen) gene high-resolution genotyping method based on an Illumina GA sequencing technology and a primer label for the method.

The invention discloses a human ALK fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK fusion gene types A01 to A16 and Taqman probes corresponding to the gene types. As many as sixteen fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample RNA once, inverse transcription and PCR amplification are carried out in one closed tube reaction system, and the pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion gene detection primer set and the detection kit are safe and nontoxic, and cost is low.

The invention discloses a light control and neural information detection system. The system comprises a light stimulation control module, an electrochemical detection module, an electrophysiological detection module, a synchronous data acquisition module, a main control module and a synchronous control module, wherein the light stimulation control module can be combined with the optogenetics technology to carry out light control via laser fibers or LED lamp electrodes; the electrochemical detection module is used for detecting neurochemical signals; the electrophysiological detection module is connected with a microelectrode array and is used for detecting neural electrophysiological signals; the synchronous data acquisition module is used for synchronously acquiring neural electrochemical and electrophysiological signals; the main control module is used for carrying out synchronous coordinated control on light stimulation and neural signal detection and completing data communication; the synchronous control module is used for completing the functions of man-machine interaction, data processing, display, analysis and storage, and the like. The system can be used for solving the problems that light control and signal detection are difficult in synchronous and experiment operation is fussy in the research on neurosciences.

The invention discloses a preparation method of a hollow carbon material which has dodecahedron morphology and is doped with hollow cobalt phosphide nanoparticles in situ and an application of the hollow carbon material in catalytic electrolysis of water for hydrogen production, and belongs to the technical field of catalytic electrolysis of water for hydrogen production. The method comprises specific steps as follows: (1) preparation of a metal organic framework material ZIF-67 containing cobalt ions and having dodecahedral morphology; (2) the cobalt-containing metal organic framework material ZIF-67 and a dopamine monomer are subjected to reaction, and a cobalt coordination doped hollow polymer nano-material is generated; (3) a cobalt oxide / carbon composite hollow nano-material is prepared; (4) a hollow cobalt phosphide / carbon composite hollow nanomaterial is prepared. The size of the material can be adjusted according to size of ZIF-67; in performance tests of water for hydrogen production in catalytic electrolysis, the electrode material used as a cathode shows very good electrocatalytic activity and stability. Therefore, the material has very good application prospect as the electrode material for catalyzing electrolysis of water for hydrogen production.

The invention belongs to the technical field of preparation of metal nanoparticles, and relates to a method for preparing multi-metal nanoparticles by one-step coreduction through citrate, particularly to the method for preparing the multi-metal nanoparticles with different sizes and morphologies by heating and boiling a mixed solution of a variety of metal ions and adding a citrate solution into the mixed solution rapidly, or heating and boiling the citrate solution and adding the mixed solution of a variety of metal ions into the citrate solution rapidly. The method can be used for preparing the multi-metal nanoparticles with high-efficiency catalytic activity easily and rapidly on a large scale. The gold source is HAuCl4.4H2O or HAuBr4.H2O, the palladium source is K2PdCl6 or K2PdCl4, the silver source is AgNO3 or AgF, the platinum source is K2PtCl6 or H2PtCl6.6H2O, and the citrate is sodium citrate, potassium citrate or ammonium citrate.

The invention relates to a multiple digital PCR chip and a use method thereof. The chip does not need to be driven by a precise micro-pump and be controlled by a complex micro-valve and does not need a complex macro-micro interface. The chip realizes simple, fast, low cost and large-scale autodecomposition and quantitative uniform distribution of samples. Compared with the existing commercial PCR chip, the chip greatly reduces the complexity and operating cost of the digital PCR system, simplifies the experimental operation and is free of professional training of an operator. The chip realizes the independence of each PCR reaction unit through microcavity arrays, is free of a surfactant and prevents the influence caused by an additive on the reaction system. Through integration of the isolated microcavity array zones and pre-deposition of primers corresponding to different specific target molecules in the microcavities in different zones, the chip can realize multiple detection through one step. The chip has the advantages of operation simpleness, low cost and strong multiple detection capacity and can promote development and wide application of the digital PCR technology.

The invention discloses a method for measuring residues of three phenoxy carboxylic acid pesticides in tobacco and tobacco products. The method is characterized by comprising the following steps of: digesting a sample by using acetonitrile; adding anhydrous magnesium sulfate, sodium chloride, sodium citrate and sodium hydrogen citrate to oscillate for layering; and subsequently, performing dispersive solid-phase extraction, and directly measuring the pesticide residues of 2,4-drop, 2,4,5-mucus and dicamba in the tobacco and tobacco products through liquid chromatogram-tandem mass spectrometry. According to the method, the defects of the sample treatment method in the prior art are overcome, and the sample pretreatment method and the instrument detection conditions are optimized aiming at the tobacco products; and compared with the prior art, the method has the beneficial effects that the sample pretreatment process is simple and rapid, derivatization is not required, and the method has the advantages that operation is accurate, sensitivity is high and repeatability is high.

The present invention discloses one kind of high density array type cell culturing device, storing device and experiment device. The cell culturing device includes carrier plate with ordered arranged slots of 0.3-0.4 mm depth and glass slides inside the slots. The storing device has one holder with ordered arranged openings for the circular sheets to be set in, and the openings have length smaller than the diameter of the circular glass slide, end width of 0.15-0.18 mm and middle width greater than the end width. The experiment device has experiment plate with ordered arranged notches for the sheets to be set in, and the notches have cross section shape matching the glass slides and depth of 4-6 mm. The present invention has the advantages of convenient use, high efficiency, high identity, etc.

The present invention relates to a method for detecting PKD1 gene mutation. The method utilizes sequence information of the PKD1 gene, designs nine pairs of PCR tag primers, and uses a long-chain PCR technology for targeting amplification of PKD1 gene; while amplification products are labeled, PCR products are purified and mixed for direct construction of a single SMRTBell library; a PacBio RSII sequencer is employed for sequencing; and bioinformatic analysis is carried out to obtain PKD1 gene mutation information. The invention has the beneficial effects that a simplified pair of primers is designed and combined with tag primer technology to realize respective marking of PCR products of multiple DNA samples; the amplified PCR products do not need to perform knockout and can be directly used for the library construction, and the library construction does not need extra PCR, so that a plurality of samples are mixed into one library and processed simultaneously by a PacBio RSII sequencing library construction link, and the experimental operation is greatly simplified.

The invention relates to a preparation method for a SiO<x>-C-CNT composite material of a lithium-ion battery negative electrode. According to the method, a new silicon / carbon composite structure is obtained by modes of adding a carbon nanotube conductive network (CNT) and directly pouring the CNT into a culture dish to form a film. The structure comprises a graphite framework material, amorphous SiO<x> and the CNT. With the composite material disclosed by the invention, the agglomeration effect of a nanometer silicon material is solved, and rapid attenuation of specific capacity of the battery is prevented; the amorphous SiO<x> and graphite form a SiO<x>-C structure, a huge stress effect caused by volume expansion / contraction of nanometer silicon can be buffered, and the cycle lifetime of the battery is prolonged; and the carbon nanotube is distributed in the SiO<x>-C structure, silicon and carbon can be connected very well to form a conductive network structure, the conductivity of the composite material is enhanced, and the rate performance of the battery is improved.

The invention discloses a precolumn derivatization-UPLC(ultra performance liquid chromatography)-ESI(electronic spray ion)+-MS / MS (mass spectrometry) detection method of glyphosate and glufosinate-ammonium pesticide residue in tea, sodium borate is used as a buffer salt, a relatively pure target derived product can be obtained by derivatization of glyphosate and glufosinate-ammonium and FMOC-Cl and then ultra performance liquid chromatography (UPLC) washing out and separation, and response signals can be detected by tandem mass spectrometry (MS-MS). According to the method, competition between the two pesticide residue derivatization can be eliminated, the detection effect caused by matrix effect can be effectively weakened, and the derivatization time is shortened. By experimental verification, the detection method of glyphosate and glufosinate-ammonium pesticide (herbicide) residue in tea has the characteristics of being efficient, fast and accurate, good in sensitivity and the like, is applicable in detection of the glyphosate and glufosinate-ammonium pesticide residue in tea samples of sundried green crude tea, fermented tea and substitutional tea (green food substitutional tea of agricultural industry standards of NY / T2140-2012 of the people's Republic of China) and finished tea (brick tea, Tuo tea, melon-shaped tea, cake tea, scattered tea and bagged tea and the like).

The invention relates to a method for detecting nine organophosphorus pesticides simultaneously. The method for detecting nine organophosphorus pesticides simultaneously comprises the following steps: preparing multi-recognition-site molecularly imprinted polymers, and establishing a molecularly imprinted matrix solid-phase dispersion extraction and gas chromatography combination system. Nine molecularly imprinted polymers, including dipterex, with high selection recognition performance act as adsorption functional materials, and the defects of sole recognition of traditional molecularly imprinted polymers and the like are prevented; the molecularly imprinted matrix solid-phase dispersion extraction and the gas chromatography are combined, and then the rapid and flexible detection method with a wide detection range to nine trace organophosphorus pesticides including dipterex is established. The method for detecting nine organophosphorus pesticides simultaneously provided by the invention is low in cost, simple in experiment operation and high in flexibility, and is suitable for the rapid detection on nine trace organophosphorus pesticides including dipterex in various products.