1119results about How to "Experiment operation is simple" patented technology

The invention relates to a partial-compensation aspherical reflector surface shape detection method, which comprises the following steps of demarcating partial-compensation elements to obtain demarcated phase distribution Wb; using the demarcated phase distribution Wb, sphere theory wavefront Wq and aspherical theory wavefront Wf to obtain phase distribution Wy of digital virtual sample plates; measuring an aspherical reflector surface to be measured to obtain phase distribution Wa; and reducing the phase distribution Wy from the measured phase distribution Wa to achieve surface shape error distribution Wm of the aspherical surface. The partial-compensation aspherical reflector surface shape detection method can accurately measure optical aspherical surface shapes by using a single partial-compensation element, thereby reducing design difficulty of a compensator, simplifying structure of the compensator and being clear in physical definition, simple in data processing and arithmetical operation, simple and practical in experimental operation, low in detection cost and short in test time.

The invention provides a method for detecting the character of pores of pervious concrete by processing images. The method comprises the following steps: cutting a pervious concrete sample transversely to expose an intact and smooth cutting plane which serves as an experimental surface; obtaining the images of the obverse side of the experimental surface of the brick-powder processed pervious concrete; selecting the largest regular section image of the sample from the obtained images of the obverse side, and calculating the total pixel value of the largest regular section image; blacking and whitening the selected image; extracting the pore image of the blacked and whitened image, and calculating the pixel value of each pore; calculating the total pixel value of the pores; calculating the total porosity and the distribution situation of internal pore diameters according to the obtained pixel values of the pores and the obtained total pixel value of the pores; repeating the steps, obtaining the total porosity of a plurality of cutting surfaces of the same pervious concrete sample and the distribution situation of pore diameters, and calculating the average value of the total porosity of the cutting surfaces of the same pervious concrete sample to obtain the total porosity of the pervious concrete sample and further the character of the pores of the pervious concrete.

The invention relates to a method for detecting a nonzero digit compensation light-degree optical non-spherical profile. The method comprises the following steps of: calculating the value of z(x, y)-s(x, y, r), wherein z(x, y) is the rise distribution of an aspheric surface along the direction of an optical axis, and s(x, y, r) is the rise distribution of a spherical surface closest to the aspheric surface to be detected along the direction of the optical axis; converting emergent parallel light of an interferometer into standard spherical waves by utilizing a transmission ball and using the standard spherical waves as reference spherical waves; adjusting the position of the aspheric surface to be detected to ensure that the circle center of the spherical surface closest to the aspheric surface to be detected coincides with a focus point of converging the reference spherical waves; detecting phase distribution data of interference fringes formed by the optical waves reflected by the aspheric surface to be detected and the reference spherical waves by utilizing the interferometer; and rejecting the value of the z(x, y)-s(x, y, r) and an adjusting and positioning error epsilon (x, y) from the phase distribution data of the interference fringes to obtain the profile error distribution e(x, y) of the aspheric surface. The invention can realize the detection of large-caliber concave and convex light-degree aspheric surface profiles with high resolution and high precision and has the advantages of low detection cost and short detection cost.

The invention relates to a method for detecting surface figures of a large-aperture off-axis convex aspheric mirror. The method comprises the following steps of: performing null interferometry on reference sub-aperture regions by using computer-generated holographic elements which correspond to surface figures of the reference sub-aperture regions of an aspheric mirror to be detected; performing null interferometry on off-axis sub-aperture regions by using computer-generated holographic elements which correspond to surface figures of the off-axis sub-aperture regions of the aspheric mirror to be detected: solving the distribution of full-aperture surface figures of the convex aspheric mirror to be detected; converting coordinates of all off-axis sub-apertures in an x-y-z coordinate system into coordinates below an X-Y-Z coordinate system and obtaining the distribution of full-aperture phases of the convex aspheric mirror to be detected by calculation; and removing system adjustment errors from the distribution of the full-aperture phases to obtain the error distribution of the full-aperture surface figures of the convex aspheric mirror to be detected. The invention has the advantages of simple data processing and mathematical operation, easy experiment operation, low detection cost and short testing time.

The invention relates to a PCR primer for a human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on a NGS technology and application thereof. The PCR primer for the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence comprises at least one capturing primer pair, and sequences of a forward primer and a reverse primer of each capturing primer pair respectively comprise a specific sequence and a linker sequence connected with a terminal 5' of the specific sequence. The PCR primer used for amplifying the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on the NGS technology and application thereof have the advantages that experiment operations related in the whole method are simple, only a PCR reagent and a primer combination are related, cost is low, and a ditag sequencing linker sequence is introduced for distinguishing different samples, so that high throughput sample sequencing detection can be realized, and gene detection support can be effectively provided for risk assessment on human breast cancer and other BRCA susceptible gene-related hereditary tumours or targeted medication specific to BRCA mutation.

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention relates to beaded electrospun nanofiber immobilized saccharomycete and a preparation method thereof. Nanofiber comprises electrospun polymer or macromolecules, a solvent and a saccharomycete; prepared mixed solution is placed in an injector and is spun with an electrospinning instrument, microorganisms are introduced into the nanofiber by dispersion of the saccharomycete in a spinning solution, the advantages of the nanofiber such as high specific area, high porosity, large length-diameter ratio and bulkiness and porousness are combined, in-situ immobilization is achieved for saccharomycete cells, an active organic/microorganism composite is obtained, and a function similar to a multi-enzyme bioreactor is enabled. A product and preparation method of the invention have the advantages such as high processing efficiency, high reacting speed, ease of control, high antifouling capacity, continuous reusability, and ease of product separation. The beaded electrospun nanofiber immobilized saccharomycete is applicable to the fields such as biological catalysis, filtering and adsorption, drug delivery and tissue engineering.

The invention discloses a molecular marker auxiliary selection method for TYLCV breeding, and belongs to the technical field of vegetable disease resistance breeding. The method comprises the following steps: (1) extracting DNA from breeding separation generation materials; (2) sieving SCAR molecular markers of anti-TYLCVD genes; (3) performing double PCR amplification and electrophoretic analytic detection on the DNA; and (4) evaluating the resistance of the breeding separation generation materials according to the detection result. The method can accurately and quickly detect whether any generation material in the breeding process is polymerized with the anti-TYLCV genes in a laboratory so as to obviously shorten the period of disease resistance breeding and reduce the breeding scale. Moreover, the method has the advantages of simple and convenient operation, time conservation, labor conservation, cost reduction and the like, reduces the workload, improves the sieving efficiency of resistant materials, accelerates the disease resistance breeding process, and can be popularized and applied to tomato breeding units.

The invention provides a transition metal chalcogenide nanosheet material and a preparation method thereof, a battery anode material, a secondary battery and an application thereof, and belongs to thetechnical field of transition metal chalcogenides. The invention provides a preparation method of a transition metal chalcogenide nanosheet material, comprising the following steps: dissolving raw materials in an aqueous solution of soluble salt, removing moisture to obtain a solid, sintering the solid, and washing to obtain a transition metal chalcogenide nanosheet material, wherein the raw materials include a chalcogen precursor and a transition metal precursor. According to the preparation method, the soluble salt is used as a template, is inexpensive, is environmentally friendly and is easy to remove in post-treatment, and the obtained transition metal chalcogenide nanosheet material has a thinner sheet thickness. Compared with a traditional silica template method or a hydrothermal method, the preparation method of the invention greatly shortens the synthesis cycle, is simple and feasible, is controllable, has characteristics of high yield and simple experimental operation, and issuitable for large-scale production.

The invention relates to the technical field of electric power, in particular to a system and method for testing the electric conductivity of a metal wire. The system comprises a direct-current power supply, a wire clamping system and a test system. The output end of the direct-current power supply is in series connection with the metal wire to be tested through a fixture. The metal wire to be tested is connected to an optical platform through a fixture. A first camera and a second camera are respectively and electrically connected with a computer. A multiple-pass digital multimeter V is connected with the metal wire to be tested and is further electrically connected with the computer. A multiple-pass digital multimeter A is connected with the metal wire to be tested through the direct-current power supply. Due to the adoption of the system and method for testing the electric conductivity of the metal wire, the electric conductivity of a metal wire of any length and section shape can be tested without considering the effect of the temperature on the resistance of the wire to be tested, and experiments are easy and quick to operate. The system and method greatly improve working efficiency, obviously improve testing accuracy and are suitable for all kinds of novel energy-saving metal wires.

The invention provides an HLA (Human Leukocyte Antigen) gene high-resolution genotyping method based on an Illumina GA sequencing technology and a primer label for the method.

The invention discloses a human ALK fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK fusion gene types A01 to A16 and Taqman probes corresponding to the gene types. As many as sixteen fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample RNA once, inverse transcription and PCR amplification are carried out in one closed tube reaction system, and the pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion gene detection primer set and the detection kit are safe and nontoxic, and cost is low.

The invention discloses a preparation method of a hollow carbon material which has dodecahedron morphology and is doped with hollow cobalt phosphide nanoparticles in situ and an application of the hollow carbon material in catalytic electrolysis of water for hydrogen production, and belongs to the technical field of catalytic electrolysis of water for hydrogen production. The method comprises specific steps as follows: (1) preparation of a metal organic framework material ZIF-67 containing cobalt ions and having dodecahedral morphology; (2) the cobalt-containing metal organic framework material ZIF-67 and a dopamine monomer are subjected to reaction, and a cobalt coordination doped hollow polymer nano-material is generated; (3) a cobalt oxide/carbon composite hollow nano-material is prepared; (4) a hollow cobalt phosphide/carbon composite hollow nanomaterial is prepared. The size of the material can be adjusted according to size of ZIF-67; in performance tests of water for hydrogen production in catalytic electrolysis, the electrode material used as a cathode shows very good electrocatalytic activity and stability. Therefore, the material has very good application prospect as the electrode material for catalyzing electrolysis of water for hydrogen production.

The invention belongs to the technical field of preparation of metal nanoparticles, and relates to a method for preparing multi-metal nanoparticles by one-step coreduction through citrate, particularly to the method for preparing the multi-metal nanoparticles with different sizes and morphologies by heating and boiling a mixed solution of a variety of metal ions and adding a citrate solution into the mixed solution rapidly, or heating and boiling the citrate solution and adding the mixed solution of a variety of metal ions into the citrate solution rapidly. The method can be used for preparing the multi-metal nanoparticles with high-efficiency catalytic activity easily and rapidly on a large scale. The gold source is HAuCl4.4H2O or HAuBr4.H2O, the palladium source is K2PdCl6 or K2PdCl4, the silver source is AgNO3 or AgF, the platinum source is K2PtCl6 or H2PtCl6.6H2O, and the citrate is sodium citrate, potassium citrate or ammonium citrate.

The invention relates to a multiple digital PCR chip and a use method thereof. The chip does not need to be driven by a precise micro-pump and be controlled by a complex micro-valve and does not need a complex macro-micro interface. The chip realizes simple, fast, low cost and large-scale autodecomposition and quantitative uniform distribution of samples. Compared with the existing commercial PCR chip, the chip greatly reduces the complexity and operating cost of the digital PCR system, simplifies the experimental operation and is free of professional training of an operator. The chip realizes the independence of each PCR reaction unit through microcavity arrays, is free of a surfactant and prevents the influence caused by an additive on the reaction system. Through integration of the isolated microcavity array zones and pre-deposition of primers corresponding to different specific target molecules in the microcavities in different zones, the chip can realize multiple detection through one step. The chip has the advantages of operation simpleness, low cost and strong multiple detection capacity and can promote development and wide application of the digital PCR technology.

The invention relates to a method for detecting nine organophosphorus pesticides simultaneously. The method for detecting nine organophosphorus pesticides simultaneously comprises the following steps: preparing multi-recognition-site molecularly imprinted polymers, and establishing a molecularly imprinted matrix solid-phase dispersion extraction and gas chromatography combination system. Nine molecularly imprinted polymers, including dipterex, with high selection recognition performance act as adsorption functional materials, and the defects of sole recognition of traditional molecularly imprinted polymers and the like are prevented; the molecularly imprinted matrix solid-phase dispersion extraction and the gas chromatography are combined, and then the rapid and flexible detection method with a wide detection range to nine trace organophosphorus pesticides including dipterex is established. The method for detecting nine organophosphorus pesticides simultaneously provided by the invention is low in cost, simple in experiment operation and high in flexibility, and is suitable for the rapid detection on nine trace organophosphorus pesticides including dipterex in various products.