30 results about "Extracellular matrix" patented technology

This invention belongs to the field of biomedical technology and discloses the application of KDELR3 inhibitors in the preparation of drugs for the prevention and / or treatment of individual fibrotic diseases. In a wild-type mouse model of pulmonary fibrosis, compared with the control group receiving intratracheal injection of Scr siRNA, intratracheal injection of KDELR3 siRNA significantly reduced the degree of fibrosis in the mouse lung tissue. KDELR3 inhibition significantly reduced collagen deposition and improved lung tissue structural damage. Further mechanistic studies showed that KDELR3 inhibitors can inhibit the differentiation of fibroblasts into myofibroblasts, thereby reducing the production of fibrosis-related extracellular matrix. These results indicate that KDELR3 is a key molecule regulating the occurrence and development of fibrosis, and drugs that can inhibit KDELR3 expression or activity can be used to prevent and treat various fibrotic diseases, including pulmonary fibrosis, laying the foundation for the development of novel drugs that are effective and safe in treating fibrotic diseases.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of a composition in the preparation of a drug for treating diabetic wounds. The composition comprises the following components: *Artemisia capillaris* exosomes, epi-suberol, and 3-hydroxymorinogenone, with a mass ratio of 1:0.1-0.25:0.02-0.05. In this composition, *Artemisia capillaris* exosomes can penetrate the thickened inflammatory exudate layer and glycosylated extracellular matrix of diabetic wounds, reshaping the wound's immune repair microenvironment, promoting fibroblast activation and proliferation, regulating collagen synthesis and deposition, and reducing scar hyperplasia. 3-hydroxymorinogenone can increase vascular endothelial growth factor, promote angiogenesis, and thus promote the repair and healing of diabetic wounds. The synergistic effect of *Artemisia capillaris* exosomes, epi-suberol, and 3-hydroxymorinogenone in this composition significantly shortens the healing cycle of difficult-to-heal diabetic wounds and improves the quality of wound healing.

The present application relates to the field of cosmetics, in particular to an endogenous antioxidant anti-wrinkle composition, a preparation method and application thereof, the composition comprises acetyl tetrapeptide-5, CycloRetin, collagen type 21, acetyl hexapeptide-8 and vitisins, the composition can promote the synthesis of collagen in the extracellular matrix of the dermis of the skin, and can improve the tightening and anti-wrinkle effect, human test proves that the tightening and anti-wrinkle effect of the composition is the best.

This invention discloses carboxymethyl sucrose amino acid salts, their preparation, compositions, and applications. The salts are prepared from carboxymethyl sucrose and arginine, or carboxymethyl sucrose and glycine, through a chemical reaction. The resulting salts undergo a series of processes including primary alcohol precipitation, redissolution, secondary alcohol precipitation, redissolution, tertiary alcohol precipitation, soaking, pulverization, filtration, and drying to obtain carboxymethyl sucrose arginine and carboxymethyl sucrose glycine salts. A composition is prepared by mixing these two salts. Both salts and the composition significantly inhibit the activity of matrix metalloproteinase 1 and promote the expression of type I collagen, thus delaying aging by maintaining the stability of collagen morphology and structure in the extracellular matrix. The preparation method of this invention is simple and the process conditions are mild. The product has the advantages of being weakly acidic, gentle on the skin, highly safe, and having good anti-aging effects. It can be widely used in skin care cosmetics and has promising application prospects.

The invention relates to composite hydrogel for promoting endometrial regeneration and resisting fibrosis as well as a preparation method and application of the composite hydrogel. The composite hydrogel consists of a deciduous endometrial decellularized extracellular matrix pre-gel solution and anti-fibrosis drug loaded polymer sustained-release microspheres dispersed in the deciduous endometrial decellularized extracellular matrix pre-gel solution, the material can be gelated in situ at body temperature and has a shear thinning characteristic. According to the invention, dECM with'decidualization energization 'is combined with an anti-fibrosis drug sustained release system to synergistically solve the two problems of insufficient endometrial regeneration and excessive fibrosis, and experiments prove that the dECM can significantly promote functional endometrial regeneration and restore fertility, and has important application value in drugs or medical instruments for treating intrauterine adhesion.

A platform of drug delivery based on self-lysis of ECN, a constructing method, and use of the platform are provided. The platform of drug delivery comprises ECN, a Pvhb-GST-PhiX174E circuit genetically introduced into ECN, and a drug encapsulation material. When the platform is used as an anti-cancer drug carrier, the platform delivers a high concentration of chemotherapeutic drugs to a tumor site. Additionally, through the Pvhb-GST-PhiX174E circuit, ECN is induced to lyse in response to tumor microenvironment signals, leading to cell death in the tumor region and enhancing ECN's biosafety in vivo. Furthermore, ECN within the platform of drug delivery based on self-lysis of ECN serves as an immune adjuvant, stimulates the immune cell activity in the tumor site, triggers acute inflammation, and strengthens the anti-tumor effect, thereby achieving multi-modal anti-tumor therapy through live-cell, chemotherapy, and immunotherapy.

A three-layer structure is comprised of an extra-cellular matrix, a sheet-shaped cell culture, and a biodegradable gel layer (single layer). By virtue of this three-layer structure, breakage of the sheet-shaped cell culture due to high spray pressure is reduced. The fibrin gel may be formed on one surface of the sheet-shaped cell culture by spraying of a thrombin liquid. A production method may involve culturing cells on a temperature-responsive cell culture substrate, dripping fibrinogen onto a sheet-shaped cell culture having an extra-cellular matrix on a lower surface thereof, further spraying a thrombin liquid onto the sheet-shaped cell culture from an oblique direction, whereby the result is a three-layer structure having a fibrin gel layer only on one surface of the sheet-shaped cell culture and having an extra-cellular matrix layer on the opposite surface.

The invention relates to the technical field of skin care products, and particularly discloses an anti-aging polypeptide composition as well as a preparation method and application thereof. According to the anti-aging polypeptide composition disclosed by the invention, through a multi-target-point and multi-layer synergistic effect, efficient wrinkle smoothing, tightening and anti-aging are realized. The anti-aging polypeptide composition can act on a neuromuscular joint and a dermal matrix at the same time, and has double effects of immediate improvement and long-term repair. According to the anti-aging polypeptide composition, the active ingredients and the penetration enhancer are synergistic, and compared with exogenous supplement collagen, the anti-aging polypeptide composition can be effectively absorbed in a transdermal mode, enhance the regeneration capacity of autologous collagen, better regulate extracellular matrixes and improve the anti-aging effect of the composition, and the anti-aging polypeptide composition has the advantages that by endogenous stimulation of regeneration of structural protein such as skin collagen, the anti-aging effect of the anti-aging polypeptide composition is improved, and the anti-aging effect of the anti-aging polypeptide composition is improved. The problem of skin laxity and aging caused by loss of structural proteins such as skin collagen is thoroughly solved, and the efficient wrinkle smoothing and anti-aging effects are achieved.

The present invention relates to the use of a composition comprising a neo-synthesized extracellular matrix for the treatment of cancer, in particular for the inhibition of cancer viability, migration and proliferation.

The invention provides a method for preparing camellia polypeptide liquid with a remolded extracellular matrix protein network by a two-step enzymolysis method and application of the camellia polypeptide liquid. According to the method, the crude camellia protein is extracted through the ethanol water solution, flavone, polyphenol, saponin and tannin impurities for inhibiting protease activity in the crude protein are removed, the problem that the crude camellia protein is not subjected to enzymolysis is solved, and meanwhile the purity of the camellia protein is improved; then carrying out enzymolysis on the camellia protein by adopting a two-step protease enzymolysis method, firstly adding alkaline protease under an alkaline condition to carry out first-step enzymolysis on the camellia refined protein, and then adding neutral protease and papain to carry out second-step enzymolysis; the problem that when different proteases are simply compounded and added for use at the same time, the camellia protein cannot be subjected to enzymolysis to form the small-molecule polypeptide is solved. The camellia polypeptide liquid prepared by the invention has good repairing, anti-oxidation and anti-saccharification effects and the like, can remodel an extracellular matrix protein net, and can be applied to cosmetics.

This invention discloses a collagen-calcium chelate, its preparation method, and its application. The collagen-calcium chelate is synthesized using collagen with a specific sequence and soluble calcium salt as raw materials via an aqueous synthesis method. It combines the water-locking, skin-improving, pigment-lightening, skin-elasticity-enhancing, and whitening effects of collagen with the skin-tissue regeneration, skin-integrity-maintaining, skin-barrier-strengthening, epidermal cell proliferation and differentiation-remodeling, skin extracellular matrix-remodeling, whitening, and soothing effects of calcium ions. This results in a collagen-calcium chelate with good stability and high biological efficacy, achieving the goals of whitening, moisturizing, repairing, and soothing.

The invention discloses a targeted drug delivery system in a bladder. The targeted drug delivery system comprises a reagent A and a reagent B, the reagent A is a biological micromotor, platelets are taken as a natural carrier, and streptavidin is bridged and coupled with biotinylation modified urease to serve as a driving component to entrap active components; the reagent B is a urea solution. According to the invention, the natural characteristics of platelet chemotaxis tumor inflammation microenvironment are utilized to realize specific enrichment of motors in a bladder tumor area; after urea perfusion, urease catalyzes urea to generate gas power to push the motor to move, meanwhile, compact extracellular matrixes are slightly loosened, the motor is assisted to penetrate and reach the interior of a tumor and release drugs, and the delivery efficiency and targeting performance of the drugs in the bladder are remarkably improved.

The present invention relates to: a method for preparing a highly functional DNA fragment mixture derived from microorganisms (yeast, microalgae, lactic acid bacteria, and the like); and a functional composition comprising a DNA fragment mixture prepared thereby. Specifically, the present invention relates to the preparation of a DNA fragment mixture having a uniform size within a certain range by using a specific restriction enzyme in microorganisms (yeast, microalgae, lactic acid bacteria, and the like). According to the present invention, a functional composition may be provided, which comprises the DNA fragment mixture as a main ingredient, and exhibits excellent effects in filling an interstitial scaffold, improving skin condition, regenerating tissue, healing wounds, moisturizing, promoting ECM synthesis, inhibiting cellular senescence, forming new blood vessels, alleviating inflammation, relieving pain, treating dermatitis such as atopy, inhibiting hair loss, promoting hair growth, and promoting stem cell activity.

The invention provides an antibacterial hydrogel wound dressing with a photothermal effect as well as a preparation method and application of the antibacterial hydrogel wound dressing, and aims to solve the problems that the traditional wound dressing is insufficient in antibacterial ability and limited in inhibition of drug-resistant bacteria, and the hydrogel prepared by the invention has good functions of resisting bacteria, drug-resistant bacteria, inflammation and oxidation and promoting wound healing. The preparation method comprises the following steps: synthesizing the silver-polydopamine nanoparticles, preparing an extracellular matrix hydrogel prepolymer, and combining the silver-polydopamine nanoparticles with the polymyxin B to form the final hydrogel. The hydrogel disclosed by the invention can realize sterilization treatment through a photothermal effect under the irradiation of near-infrared light, has relatively good biocompatibility, temperature-sensitive characteristic and wound healing promotion effect, and is suitable for treating a wound infected by drug-resistant pseudomonas aeruginosa. Experimental results show that the hydrogel disclosed by the invention has good mechanical properties, degradability and adhesion, and shows remarkable antibacterial and healing-promoting effects in vitro and in mouse models.

The present invention concerns a method for preparing a spherical core-shell skin model, said method comprising the steps of: a) forming microdroplets of an aqueous solution comprising fibroblasts and collagen, in an oil, wherein the formed collagen microdroplets comprise at least one fibroblast per microdroplet; b) gelling at least a portion of the formed collagen microdroplets comprising said at least one fibroblast, thereby obtaining collagen beads comprising at least one fibroblast per bead; c) replacing the oil surrounding the collagen beads comprising said at least one fibroblast with a culture medium, d) contacting (i) the collagen beads comprising said at least one fibroblast with (ii) keratinocytes, and e) incubating (i) said collagen beads with (ii) said keratinocytes for a period of time enabling the formation of an external layer by said keratinocytes around said collagen beads, thereby obtaining a spherical core-shell skin model, the core comprising at least one fibroblast and the shell comprising at least one keratinocyte.

The application discloses a paeonol-loaded plant nanovesicle and application thereof in preparation of a medicine for regulating rheumatoid arthritis; the plant nanovesicle takes gardenia-derived exosome as a carrier, loads paeonol through an ultrasonic method, and is modified with a cyclic peptide RGD and a CD47 mimetic peptide on the surface, so that the plant nanovesicle can not only avoid being removed by macrophages, but also can penetrate a synovial vascular barrier and a dense extracellular matrix and be accurately delivered to an inflammatory synovial tissue, the delivery system effectively improves the stability and bioavailability of paeonol, can down-regulate a MALAT1 related signal path, inhibit abnormal activation of ERK / MAPK, and significantly reduce the expression of inflammatory factors such as IL-2, IL-6 and TNF-alpha, thereby relieving synovial inflammation of rheumatoid arthritis; the application has high targeting, long circulation, low immunogenicity and multi-dosage form adaptability, provides a novel delivery strategy of a safe and efficient active ingredient of traditional Chinese medicine, and has a wide application prospect.

The application discloses application of a Ptprj agonist GJ103 in preparation of a medicine for preventing and / or treating renal fibrosis. It is disclosed that active up-regulation of Ptprj can obviously inhibit proliferation and activation of fibroblasts caused by TGF-beta 1, and knockout of Ptprj aggravates renal fibrosis caused by UUO and promotes activation of fibroblasts. As a Ptprj small molecule agonist, GJ103 treatment not only obviously inhibits renal fibroblast activation induced by TGF-beta 1, but also obviously improves pathological damage of renal tissues caused by obstruction and ischemia and reduces deposition of extracellular matrix. The results show that Ptprj plays a protective role in the process of renal fibrosis, and its agonist GJ103 provides a new target for prevention and diagnosis and treatment of CKD.

Described here are systems, devices, and methods useful for predicting cancer based on morphological analysis of extra-cellular matrix bodies (ECMBs) from a biological fluid. A method of predicting cancer may comprise receiving image data corresponding to ECMBs separated from a biological fluid of a human or other animal subject, generating ECMB data based on the image data, and predicting a cancer parameter based on at least the ECMB data.

The present invention discloses a full-thickness skin model and its construction method and application, and relates to the technical field of biomedical materials. The present invention provides a biomatrix glue, comprising halloysite nanotubes, sodium alginate, hyaluronic acid, type I collagen, calcium chloride and DMEM powder. The biomatrix glue of the present invention can significantly promote the secretion of rich extracellular matrix by fibroblasts in the dermis, increase the sustainable mechanical strength and thickness of the dermis, and is used to simulate the dermis structure under normal and inflammatory conditions, and is conducive to the adhesion of upper keratinocytes, which is conducive to the construction of a full-thickness skin model. The full-thickness skin model constructed using the biomatrix glue of the present invention has a slow degradation efficiency, a stable structure, and biomimetic properties, and can simulate the structure of human skin; and the construction method of the full-thickness skin model is simple to operate, short in time, and low in cost. The constructed 3D skin model is stable and can be used for the treatment and mechanism research of chronic and inflammatory diseases.

The application provides a polyurethane / small intestinal submucosa composite material and a preparation method and application thereof, and belongs to the field of medicines. The polyurethane / small intestinal submucosa composite material with an asymmetric structure is prepared by using a freeze-drying and impregnation drying method and different structural forms of the same components. The composite material is porous sponge on one side and dense film on the other side, can effectively prevent leakage on the basis of guaranteeing mechanical properties, and performs full-layer repair. The composite material can adjust the component ratio according to different requirements, and then adjust the mechanical properties, so as to match the normal bladder mechanical behavior of different species. The preparation method of the composite material is simple and convenient, has high popularization, can be popularized to other polyurethane / extracellular matrix materials, and has a good application prospect.

Provided is a drug for preventing, alleviating or treating adhesion and a use thereof. A fibroblast which has been subjected to external stimulation in a body cavity contains an activated negative regulatory receptor PEAR1. The deletion of the fibroblast PEAR1 can significantly promote in vivo mucosal adhesion formation. A PEAR1 agonist can specifically inhibit fibroblast activation, synthesize an extracellular matrix, and ameliorate the adhesion lesion. By administering the PEAR1 agonist, the formation of adhesion can be effectively prevented. Also provided is a PEAR1 agonistic antibody that does not affect the repair and healing of normal tissue and does not cause the tissue to be in an anoxic state. Moreover, the antibody drug has a long half-life, is easily diffused, has few side effects, and has a convenient route of administration.

The invention discloses a paeoniflorin-loaded plant nano-vesicle and an application of the paeoniflorin-loaded plant nano-vesicle in preparation of a medicine for regulating rheumatoid arthritis. According to the plant nano-vesicle, the gardenia source exosome serves as a carrier, paeoniflorin is loaded through an ultrasonic method, cyclopeptide RGD and CD47 mimic peptide are modified on the surface, the plant nano-vesicle can be prevented from being cleared away by macrophages, and the plant nano-vesicle can penetrate through a synovial vessel barrier and a compact extracellular matrix and is accurately delivered to inflammatory synovial tissue, so that the effect of preventing inflammation is achieved. According to the delivery system, the stability and bioavailability of paeoniflorin are effectively improved, MALAT1 related signal channels can be down-regulated, ERK / MAPK abnormal activation is inhibited, expression of inflammatory factors such as IL-2, IL-6 and TNF-alpha is remarkably reduced, and rheumatoid arthritis synovial inflammation is relieved; the invention has the advantages of high targeting property, long circulation, low immunogenicity and multi-dosage form adaptability, provides a safe and efficient novel delivery strategy for traditional Chinese medicine active ingredients, and has a wide application prospect.

The invention relates to a whitening and photoaging-resistant composition containing sweet-scented osmanthus and sophora flower bud components and a preparation method of the whitening and photoaging-resistant composition, and belongs to the technical field of plant extracts. According to the invention, the sophora flower bud extract and the sweet-scented osmanthus extract are self-made, in the process of preparing the sophora flower bud extract, helicase is firstly used for enzymolysis, and then phytobacterium plantarum and aspergillus niger are used for fermentation, so that the content of active ingredients in the sophora flower bud extract can be greatly increased; the sophora flower bud extract, the roselle extract and the acerola cherry extract are combined for use, and a whitening system with a synergistic effect can be constructed through different levels. In addition, the phyllanthus emblica extract, the fish collagen peptide, the osmanthus fragrans extract and the collagen tripeptide are respectively used for inhibiting oxidative stress, maintaining steady-state balance of an extracellular matrix (ECM), improving chromatosis and balancing different targets of flora to play a remarkable anti-photoaging effect. Therefore, the prepared composition finished product has the effects of whitening and resisting light aging.

The invention belongs to the technical field of intra-tumor turbulent flow scaffolds, and particularly relates to a three-dimensional intra-tumor turbulent flow device with a repairing function and a preparation method thereof, the preparation method comprises the following steps: S1, preparing a metal turbulent flow scaffold and an extracellular matrix raw material; s2, carrying out surface modification on the metal turbulent flow bracket through oxygen plasma treatment or dilute acid etching; s3, preparing an extracellular matrix solution; s4, coating the surface of the pretreated metal stent with an extracellular matrix solution in a spraying, dipping or spin-coating manner; and S5, performing low-temperature drying or freeze drying on the coated stent, performing cross-linking treatment by using a cross-linking agent, and repeatedly eluting by using a phosphate buffer solution after cross-linking to obtain the three-dimensional intra-tumor turbulent flow device with the repairing function. According to the metal stent, the core turbulence function is guaranteed to assist tumor treatment, the ECM coating makes up the defect that metal has no biological activity, and the dual purposes of improving the tumor treatment effect and reducing the treatment side effect are truly achieved.

The invention provides a facial soft tissue defect repair device based on a bio-absorbable stent, and belongs to the technical field of medical repair, the facial soft tissue defect repair device comprises a 3D printing bio-absorbable stent and a growth factor slow-release coating; according to the 3D printing biological absorbable stent, a biodegradable polymer material without an inorganic material is adopted, a porous stent matched with a facial defect area of a patient is prepared through a 3D printing technology, and the porous stent is of a bionic micro-nano structure simulating a natural facial soft tissue extracellular matrix; the mechanical support and the three-dimensional space for the growth of facial soft tissues are provided. The stent is made of a biodegradable polymer without inorganic materials, local inflammation or foreign body reaction possibly caused by inorganic components in the face soft tissue environment can be avoided, the stent does not need to be taken out through a secondary operation due to the biodegradable characteristic, and the pain of a patient is relieved. The device promotes tissue regeneration by precisely adapting to the defect part, overcomes the defects of a traditional method, and has remarkable advantages.

Described here are systems, devices, and methods useful for predicting cancer based on morphological analysis of extra-cellular matrix bodies (ECMBs) from a biological fluid. A method of predicting cancer may comprise receiving image data corresponding to ECMBs separated from a biological fluid of a human or other animal subject, generating ECMB data based on the image data, and predicting a cancer parameter based on at least the ECMB data.

The embodiment of the invention discloses a method for constructing a bone micro-tissue by compounding a bone extracellular matrix with a vascular matrix component and application of the bone micro-tissue. The specific implementation mode of the method comprises the following steps: preprocessing a human femoral head cortical bone sample; performing freeze thawing treatment; preparing demineralized bone powder corresponding to the caput femoris cortical bone sample of the target person; preparing a bone source extracellular matrix microcarrier; preparing a vascular matrix component; mixing the vascular matrix component and the bone-derived extracellular matrix microcarrier in a basic culture medium to obtain a mixed suspension; transferring the mixed suspension to a rotary bioreactor; and performing dynamic culture treatment on the vascular matrix component and the bone source extracellular matrix microcarrier in the basic culture medium to obtain the bone micro-tissue. According to the implementation mode, the effect of the constructed bone micro-tissue can be improved, the secondary injury to a patient and the risk of complications are reduced, and the effect of repairing bone defects can be improved.

This document provides materials and methods for making and using functional (e.g., vascularized) organ models (e.g., pancreas models). For example, functional pancreas models including an ECM containing a plurality (e.g., two or more) of pancreatic islets, and a vascular network are provided.

The present application relates to a polysaccharide-based composite hydrogel and a preparation method and application thereof. The raw material of the composite hydrogel comprises: an aldehyde-modified polysaccharide containing ortho-hydroxyl groups, a decellularized extracellular matrix enzyme digestion solution, tannic acid, cannabidiol, neomycin sulfate and borneol; the multiple components are compounded, the service performance of the hydrogel can be significantly improved, and the hydrogel is particularly suitable for severe environments. The composite hydrogel has excellent mechanical properties, good biological safety, certain self-repairing performance, and higher anti-inflammatory, antibacterial and antioxidant properties, and can be applied to skin or mucosal tissue wound repair.