48 results about "Tissue remodeling" patented technology

The invention discloses a negative pressure interface material. The negative pressure interface material comprises a bioelastomer material and a biological material. The invention further discloses a preparation method of the negative-pressure interface material, rapid, continuous and large-size 3D printing of the bioelastomer material and the bioelastomer material composite material is realized by using a novel thermosetting material composite 3D printing technology and utilizing solvent compounding and solvent plastification. By utilizing the negative pressure interface material disclosed by the invention, new granulation tissues can rapidly grow by virtue of a biological scaffold under the action of negative pressure, regeneration of a new matrix is efficiently induced, wound surface defects are more rapidly filled, and optimized conditions are provided for later wound surface coverage; meanwhile, in the tissue remodeling stage, the material stent is gradually replaced by the autologous tissue through gradual degradation of the material, and finally the remodeling process is completed.

The invention discloses a preparation method and application of a porous GelMA microneedle for delivering AIE molecules based on HSF derived exosome. The preparation method comprises the following steps: S1, preparing TTMN molecules; s2, dispersing DPPC and Chol in chloroform, performing water bath evaporation at 50 DEG C to form a thin film, hydrating the thin film in PBS, incubating to enable DPPC and Chol to form a liposome structure again, and uniformly dispersing TTMN molecules in the liposome structure to obtain TL; s3, extracting an exosome from the HSF cells; s4, loading the TL into the exosome by utilizing an electroporation technology, so as to obtain TE; s5, preparing the GelMA; and S6, mixing the GelMA with the TE, so that the TE is uniformly dispersed in the GelMA to obtain a mixed solution, injecting the mixed solution into a microneedle mold, and irradiating with 405nm laser to obtain the porous GelMA microneedle. According to the invention, high-efficiency treatment of hypertrophic scars is realized, and the system has a remarkable drug delivery effect, an innovative photodynamic therapy, a high-efficiency scar tissue remodeling effect and good biological safety and stability, and has important clinical application value and market prospect.

The invention belongs to the technical field of surgical article materials, and particularly relates to a collagen membrane with an effect of inducing ordered remodeling of tissues as well as a preparation method and application of the collagen membrane. The collagen membrane is obtained by performing hot pressing treatment on a fiber membrane; the fiber membrane is formed by crossing and laminating fibers with the diameter of 0.5-15 microns; the fiber comprises collagen and poly (L-lactide-caprolactone), and the mass ratio of the collagen to the poly (L-lactide-caprolactone) is 1: (1.5-2.5). The collagen membrane provided by the invention combines collagen with good biocompatibility and a tissue repair promoting effect with biodegradable PLCL with excellent mechanical properties and good cell adhesion, not only has an extracellular matrix-like structure, but also has good structural stability, is beneficial to regulation of cell behaviors and tissue regeneration, and can be used for preparing the collagen membrane. The growth, adhesion and proliferation of host cells can be effectively promoted, so that ordered remodeling of tissues is induced.

A method of treating cancer metastasis in a subject in need thereof is provided, the method including administering to the subject a combination therapy including: (a) a vascularization and / or tissue remodeling inhibitor; (b) a suppressor of oxidative metabolism; and (c) an ion homeostasis modulator. Pharmaceutical compositions directed to the combination of therapeutic agents are also provided.

The application discloses a fish scale protein polypeptide anti-chronic heart failure efficacy evaluation system, relates to the technical field of biological active polypeptide evaluation and cardiovascular disease function analysis, and comprises a raw material processing module for preparing fish scale protein polypeptides, a CHF model construction module for animal experiments, a function detection module, and a data processing and analysis module. Multidimensional statistics and visual algorithm are adopted to integrate tissue remodeling indexes such as heart function, oxidative stress and inflammatory reaction, so that comprehensive evaluation of the efficacy of fish scale protein polypeptides is realized. The application can realize systematic, quantitative and mechanistic evaluation of the anti-chronic heart failure effect of fish scale protein polypeptides, solve the problems of single evaluation index, poor repeatability of results and lack of mechanism correlation in the prior art, and has the advantages of comprehensive evaluation, reliable results, wide application range and strong generalizability.

The present invention relates to the use of immunoregulatory macrophages for treating diseases that are associated with pathological changes of the blood vessels. The present invention particularly relates to the use of immunoregulatory macrophages for treating micro- and macroangiopathies of the lower limbs. The invention furthermore relates to the use of immunoregulatory macrophages for promoting tissue remodelling to facilitate wound healing. Pharmaceutical compositions for use in the recited treatments are also disclosed which comprise the immunoregulatory macrophages.

The present invention relates to the technical field of biogel materials, and in particular to an injectable calcium hydroxyapatite filling and repairing gel and a preparation method thereof. The present invention coats calcium hydroxyapatite on the outside of a MOF nanotube loaded with dopamine. After implantation, the calcium hydroxyapatite serving as a shell degrades and exists in the soft tissue in the form of calcium ions and phosphate ions, stimulating the production of collagen and achieving tissue remodeling. After the calcium hydroxyapatite is degraded, the MOF nanotube loaded with dopamine inside is exposed to a gel carrier and cross-linked to form a composite network structure, thereby reducing the degradation rate of the filler and achieving a long-term and full filling effect without repeated injections. The MOF nanotube encapsulated in the dopamine is evenly distributed in the soft tissue along with polymer cross-linking, and releases the contents after being degraded over time, thereby further improving the cross-linking degree of the polymer system and stimulating the production of human collagen, thereby improving the repair effect.

The present invention belongs to the field of biomedicine and relates to a preparation method of a pH-responsive dual-release hydrogel for promoting diabetic wound healing. The method comprises the following steps: (1) preparing oxidized sodium alginate; (2) preparing astilbin liposomes; (3) preparing oxidized sodium alginate solution with distilled water, adding astilbin liposomes to obtain OSA@AL solution; dissolving carboxymethylated chitosan and diclofenac sodium in distilled water to prepare CMCS@DS solution; mixing the above two solutions in equal volumes, and preparing ALD hydrogel through Schiff base reaction. The hydrogel releases DS (diclofenac sodium) in the early stage of wound formation and slowly releases AL (astilbin liposomes) in the middle stage of wound formation. By loading DS and AL in different regions of the hydrogel and utilizing its pH-responsive characteristics, the spatiotemporal controlled release of the drug is achieved, so that the hydrogel can play a role in the four stages of wound healing (hemostasis, inflammatory response, proliferation and tissue remodeling), thereby accelerating diabetic wound healing.

A tendon repair implant for treatment of a partial thickness tear in the supraspinatus tendon of the shoulder is provided. The implant may incorporate features of rapid deployment and fixation by an arthroscopic means approach that compliment current procedures; tensile properties that result in desired sharing of anatomical load between the implant and native tendon during rehabilitation; selected porosity and longitudinal pathways for tissue in-growth; sufficient cyclic straining of the implant in the longitudinal direction to promote remodeling of new tissue to tendon-like tissue; and, may include a bioresorbable construction to provide transfer of additional load to new tendon-like tissue and native tendon over time.

The present application relates to the technical field of biomedical materials, in particular to a composite gel containing core-shell structure calcium hydroxyapatite microspheres, a preparation method and application thereof. The composite gel comprises a gel carrier and microspheres dispersed in the gel carrier, the microspheres comprise a core and a shell layer coated on the outside of the core, the core is a biodegradable polymer, and the shell layer is calcium hydroxyapatite. After the core-shell structure calcium hydroxyapatite microsphere composite gel of the present application is implanted, calcium hydroxyapatite first contacts human tissues and is degraded into calcium ions and phosphate ions, stimulates collagen production and carries out tissue remodeling; then the biodegradable polymer is also metabolically degraded; and the degradation rate of calcium hydroxyapatite can control the exposure rate of the polymer microspheres, so that a long-term plumping filling effect is achieved; the gel has suitable viscoelasticity, can uniformly suspend the composite microspheres, and after injection into the human body, the microspheres are also uniformly dispersed in the gel.

The application belongs to the technical field of medicines, and particularly relates to application of alpha-asarone in preparation of a medicine for treating allergic rhinitis. The application aims at the problems of single drug target, prominent safety hazards and inability to block pathological progress from the root in the existing medicines for treating allergic rhinitis, takes alpha-asarone as an active ingredient, inhibits excessive activation of an inflammation pathway by activating an Nrf2 / HO-1 signal axis, and bidirectionally regulates expression balance of GPX4 and ACSL4 to inhibit ferroptosis and damage of a nasal epithelial barrier. Animal experiments prove that the alpha-asarone can significantly reduce allergic behaviors of model mice, relieve inflammation of nasal mucosa, reverse tissue remodeling, has high safety, and can be prepared into oral or nasal preparations, and provides a new treatment scheme with multiple target points for allergic rhinitis.

The invention discloses schizophyllan, which has two components with molecular weights of 1.54 * 10 < 7 > Da and 1.85 * 10 < 4 > Da respectively, and consists of glucose, mannose, galactose and fucose, and the molar ratio of glucose to mannose to galactose to fucose is 86: 8: 5: 1. The schizophyllan is a heterogeneous polysaccharide with glucose as a main component, and can promote proliferation of HaCaT cells and HUVEC cells. Meanwhile, the schizophyllan can remarkably promote the healing speed of a full-thickness skin wound model mouse wound and has excellent effects in the aspects of epithelium reformation, granulation tissue formation and tissue remodeling, and when 4 mg / mL of schizophyllan is used for treating the mouse wound, the healing rate reaches 96.65%.

The invention relates to the field of microorganisms, and discloses a bifidobacterium longum BN-BB01 strain and application thereof in improvement of helicobacter pylori infection. The preservation number of the bifidobacterium longum BN-BB01 provided by the invention is CGMCC No.34432, and the bifidobacterium longum BN-BB01 can inhibit the activity of urease and the colonization and reproduction of helicobacter pylori, and also can regulate intestinal metabolism, so that the content of beneficial fatty acids in intestinal tracts is increased, and the content of corrupted fatty acids is remarkably reduced. Besides, the complete living bacteria body of the bifidobacterium longum also has a tissue remodeling capability, can specifically reverse gastric mucosa fibrosis caused by helicobacter pylori infection, and provides a new treatment approach for solving intestinal barrier and gastric mucosa structure damage caused by helicobacter pylori infection.

The invention relates to a fusion polypeptide for diabetes wound repair and a pharmaceutical composition thereof, and discloses a sequence design and a preparation method of the fusion polypeptide FP-DW01-Chol, and a temperature-sensitive hydrogel composition containing the polypeptide and MitoQ. The fusion polypeptide contains a TREM2 agonist domain, an AGE-RAGE blocking domain, an MMP9 recognition cleavage site, an Angiopoietin-2 mimic peptide fragment and a cholesterol modification terminal, and can activate anti-inflammatory polarization of macrophages, block a chronic inflammation pathway and respond to a wound enzyme environment to release an angiogenesis promoting fragment. The pharmaceutical composition takes poloxamer 407 / 188 as a temperature-sensitive carrier, realizes cooperative delivery of polypeptide and MitoQ, and has the advantages of slow release, skin retention and stability. In-vitro and db / db mouse model experiments prove that the composition can remarkably promote high-glucose damaged cell activity recovery, inhibit inflammatory factor release, accelerate wound closure, promote angiogenesis and tissue remodeling, provides a multi-mechanism synergistic and local long-acting treatment scheme for diabetic refractory wounds, and has important clinical transformation value.

The invention discloses composite hydrogel for promoting healing of chronic wound surfaces of diabetes and a preparation method of the composite hydrogel. The method comprises the following steps: (1) preparing a black phosphorus nanosheet BPNs suspension; (2) preparing aminated polyethylene glycol modified BPNs; (3) preparing methacrylated gelatin powder; and (4) preparing the composite hydrogel for promoting the healing of the chronic wound of diabetes mellitus. According to the preparation method, aminated polyethylene glycol is loaded on black phosphorus nanosheets in an electrostatic self-assembly action mode, the composite particles are excellent in antioxidant stability, and the loading capacity is stable and controllable; polyphosphates released by photo-thermal excitation response are used as biological energy precursors, in-situ regeneration of intracellular adenosine triphosphate is driven through adenylate kinase mediated phosphoryl transfer reaction, and energy metabolism disorder of a diabetes wound microenvironment is relieved fundamentally, so that migration of damaged cells, endothelial vascularization and tissue remodeling are promoted synergistically, and the wound healing effect is improved. The chronic wound healing is accelerated.

The invention relates to a system and method for tissue remodeling. Systems and methods for remodeling tissue, such as cardiac tissue. In some configurations, the systems and methods involve reshaping a mitral valve of a patient. Systems and methods may include a first tissue anchor, a second tissue anchor, a suture extending between the tissue anchors, and at least one suture lock that secures the suture or fixes the length of the suture relative to the tissue anchors. The systems and methods may also include a suture trimmer that may trim an excess portion of the suture. In some configurations, the suture lock is reversible to allow for iterative adjustment of the reshaping.

Systems and methods for reshaping tissue, such as cardiac tissue. In some configurations, the systems and methods relate to reshaping a patient's mitral valve. The systems and methods may include a first tissue anchor, a second tissue anchor, a suture extending between the tissue anchors, and a suture lock that secures the suture or fixes the length of the suture relative to the tissue anchors. The systems and methods may also include a suture trimmer that can trim excess portions of the suture. In some configurations, the suture lock is reversible to allow iterative adjustments to the reshaping.

The invention discloses an extracellular vesicle with an immunoregulation function and a preparation method of tolerance dendritic cells induced by the extracellular vesicle, and the extracellular vesicle is prepared from a conditioned medium collected by culturing stem cells on a large scale by a serum-free stem cell medium with definite components under a low oxygen partial pressure condition. Inducing the immature dendritic cells to obtain tolerant dendritic cells; the serum-free culture medium with determined components is provided for large-scale culture of stem cells, so that a large number of extracellular vesicles prepared by separation and purification of the conditioned culture medium can be collected and can be used for inducing to obtain tolerant dendritic cells, and the extracellular vesicles have the effects of resisting anaphylaxis, inhibiting inflammation, regulating immunity and improving tissue remodeling. Under the condition of low oxygen partial pressure, the tolerance dendritic cells induced by the stem cell-derived extracellular vesicles cultured by a serum-free stem cell culture medium with definite components are prepared into a biological agent, and the biological agent is applied to drugs for treating allergic diseases.

Tissue derived porous matrices for treating wounds are provided, as well as methods for making and using them. The tissue derived porous matrices comprise processed tissue of any of several types, such as dermis, adipose, etc., and have a plurality of interconnected pores which allow fluid flow through the matrices. The tissue derived matrices are biocompatible resorbable matrices which remodel with native tissue and facilitate and enhance cell infiltration and tissue ingrowth into the matrices during the wound healing process, thereby enhancing wound healing and tissue remodeling when implanted into a patient. The tissue derived matrices are useful with reduced or negative pressure wound healing methods and systems, without the need to repeatedly revisit the treatment site and remove previously implanted matrices.

The present invention relates to a metal-organic framework for inhibiting the activity of M2 macrophages, and a pharmaceutical composition comprising same, wherein the metal-organic framework of the present invention is non-toxic to cells and can exhibit an effect of inhibiting the activity of M2 macrophages. More specifically, the metal-organic framework of the present invention can be provided as an anticancer composition for tumor-related macrophage-mediated diseases, particularly solid cancer, through the inhibition of M2 macrophages capable of increasing the expression of genes associated with parasite invasion, tissue remodeling, and tumor proliferation (immunomodulatory function), and can also be provided as a pharmaceutical composition for treating chronic infectious diseases, liver cirrhosis, obesity-related metabolic diseases, scar formation and idiopathic lung diseases.

A system for wound repair, coping with complex "inflammation storm" microenvironment, and double challenge of infection and inflammation, meets clinical needs. By loading plant-derived exosomes with scaffold characteristics of acellular matrix, a bioactive nanofiber dressing is constructed, which has physical protection and high porosity characteristics. Mechanism research shows that the system can continuously release by down-regulating pro-inflammatory cytokines to reshape the immune microenvironment, while showing strong broad-spectrum antibacterial activity against staphylococcus aureus, escherichia coli and pseudomonas aeruginosa. In addition, angiogenesis is significantly promoted and epithelial regeneration is accelerated through the HIF-1ɑ / VEGF pathway. In vivo evaluation confirms that compared with the commercial treatment scheme, the scheme of the present application can significantly improve the healing effect of burn wounds and achieve better tissue remodeling, providing a cost-effective solution for the management of severe burns.

The invention provides a recombinant collagen-polysaccharide dressing and a preparation method thereof. The dressing comprises recombinant humanized III-type collagen doped with 5-20% of VII-type or I-type collagen, and sodium hyaluronate, modified chitosan, a humectant and an auxiliary agent, the collagen is partially PEGylated and forms a layered network with the polysaccharide; the inner layer is collagen-HA-sacrificial network gel, and anti-fibrosis lipidosome is encapsulated so as to realize short-term release and fitting repair; the outer layer is a modified chitosan-HAMA / PEG film forming layer, has relatively high crosslinking density and slow degradation performance, provides mechanical support, has interfaces connected through hydrolyzable Schiff-base or ester bonds, and is self-dissociated within 7-10 days to promote tissue remodeling, and the dressing has good wettability, film forming-gel matching performance and long-acting stability.

The present invention relates to a peptide specifically activating signal transduction by targeting fibroblast growth factor receptor (FGFR), an FGFR agonist comprising the peptide, a pharmaceutical composition for preventing or treating diseases associated with decreased FGFR activity, and a cosmetic composition. An FAP1 peptide according to the present invention is a potent FGFR-activating peptide, which exhibited an effective concentration similar to that of bFGF protein, and showed the ability to promote wound healing and support tissue remodeling in vivo. Considering specificity to FGFR and the ability to promote wound healing , FAP1 can be used as a cost-effective alternative to the bFGF protein in therapeutic fields.

The present application relates to the technical field of intelligent medical monitoring, in particular to a burn wound healing process real-time monitoring method and system, comprising the following steps: collecting oxygen saturation data to construct a microcirculation blood flow feature set, performing texture tracking on a wound image to construct a global deformation vector field, fitting an edge line and combining the deformation field to obtain a net proliferation migration vector field, constructing a recursive feature matrix and calculating a laminar flow property and a capture time index, and outputting an evaluation result, in the present application, by combining microcirculation blood flow features and deformation field mapping, background motion interference is eliminated, net proliferation migration behavior is accurately quantified, a recursive feature matrix is constructed by mapping time series signals to phase space, laminar flow property and capture time index in state evolution are identified, multi-dimensional state determination is performed by fusing physiological blood flow parameters and morphological contraction coefficients, non-contact objective quantitative analysis of the healing process is realized, multi-dimensional pathological grading standards are established, and the dynamic trend of tissue remodeling is accurately reflected.

To provide systems and methods for using cooling to trigger desirable effects of increased vasculature and / or development of new collagen in biological tissue.SOLUTION: The systems and methods provide a cooling treatment system configured to provide bulk or fractionated cooling at either ablative temperatures or intermediary remodeling temperatures to promote tissue remodeling by inducing increased vasculature and / or formation of new collagen.SELECTED DRAWING: Figure 1

Provided herein are methods, compositions, and uses relating to inhibitors of stem cell factor. For example, provided herein are antibodies targeting stem cell factor and methods for treating fibrotic and tissue remodeling diseases.

Systems and methods for remodeling of tissue, such as heart tissue. In some configurations, the systems and methods are directed toward remodeling of the mitral valve of a patient. The systems and methods can include a first tissue anchor, a second tissue anchor, a suture that extends between the tissue anchors, and a suture lock that secures the suture relative to at least one of the tissue anchors or fixes a length of the suture. The systems and methods can also include a suture trimmer that can trim an excess portion of the suture. In some configurations, the suture lock is reversible to allow for iterative adjustments of the remodeling.

The invention relates to the technical field of biological medicine. The invention provides a poria peel triterpene modified product-sodium alginate hydrogel dressing with a stage specific repair function as well as a preparation method and application of the poria peel triterpene modified product-sodium alginate hydrogel dressing. Three kinds of poria peel triterpene differentiation modified products (PCTT / SD, PCNT / SD and PCWT) are obtained through a solid dispersion technology or a salt forming technology, and after being loaded on hydrogel, the three kinds of poria peel triterpene differentiation modified products show clear and complementary stage specific treatment advantages in the complex wound healing process: PCNT / SD / Gel strongly regulates and controls early inflammation and accelerates initial repair; pCTT / SD / Gel significantly promotes synthesis and deposition of collagen in the middle stage; and the PCWT / Gel efficiently drives later epithelization, angiogenesis and tissue remodeling. Accurate time sequence regulation and control of the wound healing process are achieved, an innovative treatment strategy is provided for refractory wounds such as diabetic foot ulcer, and the additional value of the poria cocos peel by-product is remarkably increased.

The application discloses a short peptide for TRPM7 channel, a vaccine and pharmaceutical application of the short peptide for preventing and treating atrial fibrillation, and belongs to the technical field of biological medicine. The short peptide can be coupled with a carrier KLH by a glutaraldehyde coupling method to prepare a conjugate, and the conjugate is supplemented with an adjuvant to form a vaccine. The vaccine can induce the body to produce high-specificity and high-titer antibodies through active immunization, the antibodies can block the TRPM7 channel, inhibit calcium influx of atrial muscle cells and atrial fibroblasts, reduce calcium overload of cytoplasm, inhibit atrial electrical remodeling and tissue remodeling, and prevent and treat atrial fibrillation. The application provides a pharmaceutical composition containing the short peptide, the conjugate and the adjuvant, and a polyclonal antibody obtained by immunizing a non-human animal with the vaccine. Compared with existing chemical drugs, the application has the advantages of high targeting, long-acting, good safety, low price and the like, provides a new active immunotherapy strategy for atrial fibrillation patients, and has a wide clinical transformation prospect.

Hydrogel compositions are described with dynamic and tunable properties, mimicking extracellular matrix, for use in enhancing tissue remodeling, tissue engineering or wound healing, among others.