10 results about "Porous scaffold" patented technology

This invention relates to the field of biomedical materials, and discloses a biomimetic piezoelectric composite membrane, its preparation method, and its application. The biomimetic piezoelectric composite membrane comprises a dense layer and a porous layer. The dense layer contains a first L-polylactic acid and a first D-polylactic acid, while the porous layer contains a second L-polylactic acid, a second D-polylactic acid, and hydroxyapatite. This invention employs a stepwise continuous electrospinning technique to obtain a biomimetic piezoelectric composite membrane with an integrated dense-porous-porous layer structure. The dense layer prevents soft tissue ingrowth into bone defect areas, while the porous layer acts as a porous scaffold, supporting and guiding new bone growth. Simultaneously, it generates electrical signals in response to mechanical stress, promoting osteogenic differentiation. Furthermore, the porous layer is composited with nano-hydroxyapatite, providing essential calcium and phosphorus elements for osteogenic formation. This synergistic effect enhances alveolar bone regeneration and repair capabilities from multiple perspectives, including electrical, chemical, and topological aspects.

This invention discloses a biomimetic corncob scaffold with piezoelectric, antibacterial, osteogenic, and healing-promoting functions, comprising: extraction of corncob cellulose nanofibers (CNF); preparation of 3D printing ink; 3D printing of a porous scaffold; loading of antimicrobial peptides and BMP-2 onto a GelMA+PAAM composite hydrogel; and composite preparation of the scaffold and hydrogel. Under external stimulation, the scaffold generates a microcurrent / surface charge, directly disrupting bacterial cell membranes; it avoids drug release and resistance issues, employing a physical mechanism of action with a sustainable response. The microcurrent / electric field generated by the piezoelectric effect simulates the bioelectric environment of natural bone, directly stimulating osteoblast proliferation and differentiation, and promoting mineralization; it mimics the natural anisotropic porous structure of the corncob, facilitating nutrient transport and cell migration; the piezoelectric material itself provides both antibacterial and osteogenic functions, achieving synergistic and unified mechanisms; it is suitable for harsh environments, biodegradable, and has flexible activation methods.

The present application relates to a kind of porous scaffold for repairing bone defect loaded with black phosphorus active oxygen elimination and slow-release drug and its preparation method;The scaffold includes the amino cation polymer grafted with the phenylboronic acid (PBA) and cyclodextrin (CD) that can eliminate active oxygen, black phosphorus nanosheet (BP) and scaffold material poly (lactic acid-glycolic acid) (PLGA).The porous scaffold loads a large number of black phosphorus nanosheet that can be hydrolyzed into bone repair nutrients, and the porous structure provides sufficient space for cell migration and growth, realizes the good repair of bone.Active oxygen elimination ability, can eliminate active oxygen in diabetic microenvironment.In addition, cyclodextrin can load a variety of drugs, such as osteogenic differentiation induction drug, and realize the slow release of drug.The porous scaffold has good biocompatibility, and the degradation product is harmless to human body, can be used for the tissue repair of bone defect, can be widely applied in the field of biological medicine and tissue engineering, and has been proved to have good repair ability in the diabetic rat skull defect model.

This invention provides a method for preparing an antimicrobial peptide composite material, comprising: S1 preparing a β-TCP / HyA porous substrate; S2 synthesizing mesoporous polydopamine (MPDA); S3 dispersing the β-TCP / HyA porous scaffold and the mesoporous polydopamine in deionized water for a second crosslinking reaction to form a β-TCP / HyA / MPDA composite suspension; and then adding the antimicrobial peptide KR to the β-TCP / HyA / MPDA composite suspension to induce a binding reaction, thereby obtaining the antimicrobial peptide composite material. This invention crosslinks β-tricalcium phosphate (β-TCP), hyaluronic acid (HyA), and mesoporous polydopamine (MPDA) to construct a multifunctional composite substrate that combines physical support, biocompatibility, and high-efficiency loading. The mesoporous structure of MPDA provides numerous anchoring sites for the antimicrobial peptide KR. Through MPDA adhesion and covalent fixation strategies for the antimicrobial peptide, high-density and high-activity loading of KR molecules is achieved, effectively solving the problems of easy detachment and "fixation leading to inactivation".

The application relates to the technical field of biomedical materials, and specifically discloses a preparation method of a double-crosslinked 3D-printed bone repair scaffold. The scaffold is made of a biphasic calcium phosphate matrix and a gelatin methacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphasic calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; then, carrying out double-crosslinking treatment of calcium chloride solution and glutaraldehyde solution in sequence to obtain a scaffold matrix with enhanced mechanical properties; then, preparing gelatin methacrylate hydrogel loaded with modified teriparatide, and compounding the gelatin methacrylate hydrogel with the scaffold matrix; and finally, obtaining the final product after blue light crosslinking and solidification. The bone repair scaffold has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. In addition, the preparation method is controllable and has good repeatability, the structure of the scaffold is accurately controlled through the 3D printing technology, and a new approach is provided for the preparation of personalized bone repair materials.

This invention discloses a highly active ceramic-collagen composite bone scaffold and its preparation method, belonging to the field of bone repair material preparation technology. Quaternized ammonium inorganic material inclusions, photosensitive resin, and dispersant are mixed and ball-milled to obtain a mixed slurry. The mixed slurry is then printed to obtain a bioceramic porous scaffold green body. The bioceramic porous scaffold green body is sintered to obtain a bioceramic porous scaffold. The bioceramic porous scaffold is then vacuum-mixed with collagen fiber slurry, followed by post-treatment to obtain a ceramic-collagen composite scaffold. The ceramic-collagen composite scaffold is then impregnated with rhBMP-2 solution to obtain the highly active ceramic-collagen composite bone scaffold. The preparation method of this invention not only solves the mechanical properties of the prepared bone scaffold but also achieves good osteogenic effects.

This invention discloses a method for preparing a porous electrospun scaffold based on polycaprolactone and acetylglucan, and its application. The preparation method includes: dissolving glucan in an organic solvent, reacting it with pyridinium 4-methylbenzenesulfonate and 2-methoxypropylene under nitrogen protection, followed by termination, precipitation, centrifugation, washing, and freeze-drying to obtain acetylated glucan; subsequently, dissolving it and a polymer separately in a mixed solvent of dichloromethane and N,N-dimethylformamide, and spinning it into a film using a bilayer coaxial electrospinning technique to prepare a porous scaffold. This scaffold exhibits strong stability, excellent biocompatibility, and the ability to promote tissue repair, making it suitable for the treatment of spinal cord injuries. In particular, it can precisely target the injured site via in-situ drug delivery, reducing the impact on normal tissues and achieving highly efficient repair effects.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.