The invention discloses
penicillium oxalicum, a preparation method and an application thereof. The preparation method comprises the following steps: A) antagonistic fungi separation and purification: taking a healthy
Camellia oleifera rhizosphere sample, adding the sample in a
bottle filled with
sterile water and glass bead, oscillating the
bottle under a shaking table to prepare a fluid suspension; B)
primary screening: employing a plate confrontation for screening the fungi for
antagonism of Camellia
pathogen, placing a
pathogen cake and an antagonistic fungi cake on a flat center line, respectively keeping distances to the flat center by 1.5 cm, after constant temperature culture, screening the strains with a bacteriostasis
diameter being greater than 8mm, and C) secondary screening: culturing the strains through
primary screening by a
liquid medium, centrifuging the strains and removing
thallus, taking an aseptic filtrate and a disinfected PDA
solid medium and mixing the aseptic filtrate and the disinfected PDA
solid medium uniformly, pouring on plate, inoculating the Camellia
pathogen cake to the center of the flat, and obtaining the strain
penicillium oxalicum with strongest antagonistic action. The invention also provides an application of
penicillium oxalicum for treating or preventing and treating oil camellia diseases, and the
penicillium oxalicum has more than 90% of a bacteriostasis rate on a plurality of diseases such as oil tea anthracnose, oil tea
canker and
black spot.