The invention discloses a method for ecologically culturing high-quality red testis river crabs, which fully utilizes natural conditions and resources of ponds, natural waters, rivers, reservoirs, lakes and wetlands, starts from biological, ecological and environmental mechanisms, follows the characteristics of respective econiches occupied by various organisms in biology, is based on biological characteristics of river crabs such as life habit, feeding habit, growth and development and the like, reasonably applies ecological balance breeding technology and nutrient balance technology, builds the best ecological environment of the culture of the river crabs, and meets the nutritional need of the growth and development of the river crabs to raise the high-quality 'red testis' river crabs. The ecological culture method comprises the following steps of: culture pond pretreatment, aquatic plant cultivation, shellfish transplantation, freshwater shrimp stocking, crab seedling stocking, siniperca chuatsi delivery, water quality control, bait feeding, and disease control. The method for culturing the river crabs can produce the high-quality and high-grade 'red testis' river crabs, can greatly improve the economic benefit of crab farmers, and can greatly improve the sustainable development of crab culture industry and the ecological benefit, the economic benefit and the social benefit of crab culture.