26 results about "Artificial seawater" patented technology

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The invention belongs to the technical field of gene intervention, and provides a feeding-mediated marine mussel gene silencing method which comprises the following steps: linearizing plasmids to obtain linearized plasmids; determining a cDNA fragment of the target gene from the target mussel, and performing amplification by using the specific primer pair to obtain a target gene fragment; connecting the target gene segment with the linearized plasmid to obtain a connection product; transforming the connection product into escherichia coli, screening to obtain a positive strain, extracting target recombinant plasmids, and transforming the target recombinant plasmids into escherichia coli for expressing dsRNA to obtain engineering bacteria; engineering bacteria and microalgae are mixed according to the cell number ratio of (90-110): (1-2), a bacteria-algae feeding mixture is obtained, and mussels are fed with artificial seawater containing the bacteria-algae feeding mixture for 1-10 d. The method disclosed by the invention does not cause antioxidant system disorder or energy metabolism abnormality, and is a gene silencing strategy with higher biological safety.

This invention discloses a method for removing filamentous algae infecting hydra, comprising the following steps: (1) preparing seawater containing copper ions with a concentration of 100-250 μg / L using artificial seawater as a solvent; (2) preparing food to cultivate hydra; (3) using the prepared copper-ion-containing seawater to cultivate hydra infected with filamentous algae to remove the filamentous algae, during which the copper-ion-containing seawater is replaced every 2-3 days to maintain the copper ion concentration, at which time the hydra will degenerate and form hydra dormant bodies; (4) after the filamentous algae are completely removed, the seawater is replaced with normal seawater, and the hydra population enters the recovery period; during the recovery period, the filamentous algae do not reappear, and new hydra grow from the hydra dormant bodies after 1-16 days, and the hydra subsequently return to normal. The method provided by this invention can not only effectively remove filamentous algae that infect hydra, but also allow the hydra to quickly recover into a healthy hydra colony in normal seawater after copper ion treatment, and continuously form and release healthy jellyfish.

The invention relates to the technical field of sea squirt culture, and particularly discloses a full-artificial culture system for ciona intestinalis, a seawater preparation unit is used for purifying tap water and adding sea salt to adjust the salinity of simulated seawater; the culture unit comprises a culture pond, a seawater supply system and a drainage system; the culture pond is made of a transparent material, the seawater supply system conveys prepared seawater to the culture pond through a pipeline, and the drainage system is used for regularly replacing and discharging wastewater in the culture pond; the seawater circulating unit is used for purifying seawater in the culture system and promoting circulating flow of water in the culture system; the temperature control unit is used for controlling constant water temperature in the culture pond; the illumination control unit is used for simulating natural light to irradiate the sea squirts. By means of the artificial seawater circulation system, dependence on natural seawater is completely eliminated, and full-artificial breeding of ciona ciona is achieved. According to the system, internal circulation operation is achieved, waste of water resources is reduced, and meanwhile the risk of environmental pollution is reduced.

The utility model discloses a mariculture source water short-process treatment system, which comprises a box body, an immersed ultrafiltration membrane unit, a backwashing and medicine washing unit, a culture unit and a control unit, and the immersed ultrafiltration membrane unit, the backwashing and medicine washing unit and the culture unit are all integrated in the box body. The device is integrated in a box body, is high in integration level, takes the immersed ultrafiltration membrane assembly as a core, and is high in filtering precision and good in produced water quality; the immersed ultrafiltration membrane assembly can remove float sludge and harmful microorganisms in natural seawater, does not generate harmful by-products, does not bring harmful effects to an artificial mariculture system, and can improve the survival rate of cultured aquatic products.

The invention provides a method for efficiently separating and purifying SAR11 bacteria, belongs to the technical field of environmental microorganisms, and aims to solve the problems that the SAR11 bacteria are difficult to culture, are easily inhibited by infectious microbes and the like. The method comprises the following steps: pre-filtering a seawater sample by using a 0.45 m mixed cellulose ester film, and enriching SAR11; the method comprises the following steps: by taking artificial seawater as a matrix, adding a mixture of sodium pyruvate, methionine, glycine and vitamins with specific concentration to prepare a culture medium, inoculating a sample into a 24-pore plate, and culturing under the conditions of 17-26 DEG C and darkness / 3000 / 5000lx; the method comprises the following steps: dyeing a sample for 45 minutes by SYBR Green I, and monitoring according to SAR11 light scattering and nucleic acid characteristics by using a flow cytometer (488nm laser excitation); target cells are subjected to expanding culture to obtain single colonies, and 50% glycerol is used for low-temperature breed conservation. According to the method, the separation success rate is increased, the period is shortened, and a support is provided for researching the physiological and ecological functions and the carbon cycle effect of the SAR11.

The invention belongs to the technical field of abyssal macroorganism culture, and particularly discloses a abyssal macroorganism long-term culture system and method, and the abyssal macroorganism long-term culture system comprises a magnetic coupling type rodless seawater tank, a reaction kettle and an artificial seawater cabin. A magnetic coupling type rodless seawater cylinder comprises a cylinder body, a piston, an annular sliding block and a pressure sensor. The cylinder body is provided with a built-in cavity, and the piston is arranged in the built-in cavity in a sliding mode and divides the built-in cavity into a water inlet cavity and a water outlet cavity. The annular sliding block and the piston are magnetically coupled in a non-contact mode to form stable magnetic attraction force, the reaction kettle is provided with a water inlet end and a water outlet end, and the water inlet end, the first electromagnetic valve and the water inlet cavity are sequentially connected; the water outlet end, the second electromagnetic valve and the water outlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the third electromagnetic valve and the water inlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the fourth electromagnetic valve and the water outlet cavity are connected in sequence; according to the invention, isovolumetric replacement of new seawater and old seawater is realized, culture pressure is accurately stabilized, and biological physiological stress reaction can be effectively avoided.

To provide a method for producing artificial seawater, capable of reducing labor of a breeder or a staff of an aquarium facility or the like, and capable of being prepared in a short time.SOLUTION: A method for producing artificial seawater according to an embodiment of the present invention includes a step of preparing near-saturated salt water, a step of diluting the near-saturated salt water, and a step of dissolving trace components other than NaCl in the near-saturated salt water, wherein the trace components include at least magnesium ions, calcium ions, potassium ions, sodium ions, chloride ions, bicarbonate ions, and sulfate ions.SELECTED DRAWING: Figure 1

The invention relates to a seawater concrete hydration process regulation and control method with a carbon sequestration effect, aiming at the problems of concentrated heat release, easy cracking and the like in early hydration of large-volume seawater concrete, the early hydration process is regulated and controlled by using CO2 in a mixing stage, the main hydration heat release peak is delayed by 0.5-3 hours, the peak value is reduced by 10-30%, the accumulated heat release in 72 hours is basically unchanged or slightly reduced, and the total hydration heat release is reduced by 10-30%. Therefore, the risks of thermal cracks and shrinkage cracks are reduced, and the carbon sequestration capacity is improved. The method comprises the following steps: adding a cementing material and aggregate into a closed reactor, and stirring until dry materials are uniformly mixed; air in the reactor is pumped out, and CO2 gas is injected into the reactor to reach preset air pressure; the method comprises the following steps: injecting seawater or artificial seawater into a reactor, uniformly mixing, enabling CO2 to be subjected to carbonation reaction with calcium and magnesium ions and hydration products in a system, and forming a compact shell layer on the surfaces of clinker particles and a slurry-aggregate interface by generated nano-scale CaCO3, calcium and magnesium carbonate and magnesium-containing calcium silicate hydrate; therefore, the main hydration heat release peak value of the seawater concrete is reduced and the peak value occurrence time is delayed.

The invention discloses slag-sulphoaluminate compound gelled seawater and sea sand mortar and a preparation method thereof, and relates to the technical field of building materials. Comprising the following component raw materials: 180-220 parts of functional aggregate; 100 parts of a cementing material; 40 to 50 parts of a functional mixing liquid; the functionalized aggregate is sea sand loaded with a nanocrystalline seed layer on the surface; the cementing material is prepared from granulated blast furnace slag powder, natural anhydrite and high belite sulphoaluminate cement clinker; the functionalized mixing liquid is seawater or artificial seawater dissolved with a composite thixotropic-flocculating agent. According to the scheme, the core problems of low strength and poor durability caused by interface looseness and erosion medium permeation of the seawater and sea sand concrete are solved; and full resource utilization of the seawater, the sea sand and the slag is realized, and high performance, high durability and remarkable economic benefits are achieved.

The present invention aims to provide a closed-loop land-based aquaculture method for fish that can improve investment efficiency and also improve the rate of nitrate removal. [Solution] A closed-circuit land-based aquaculture method for farming fish on land using seawater or artificial seawater, wherein the rearing water in the rearing tank is circulated through a circulation path, the rearing water is purified as it passes through the circulation path, and the rearing water temperature is controlled to 19°C or lower, and the closed-circuit land-based aquaculture method is characterized by forming a section in the upper layer of the rearing water in the rearing tank with a partition wall that prevents fish from entering, and cultivating Ulva prolifera in the section.

This invention discloses a method for extracting and applying biomass-based surfactants for dispersing oil spills, comprising the following steps: Step 1, diatom cultivation: *Phaeodactylum tricornutum* is inoculated into artificial seawater with a salinity of 30‰, F / 2 nutrient solution is added, and it is cultured in a constant temperature and light incubator to obtain *Phaeodactylum tricornutum* in a stable concentration phase; Step 2, freeze-thaw-ultrasonic disruption: The *Phaeodactylum tricornutum* obtained in Step 1 is frozen in a refrigerator, then thawed in a water bath, and the freeze-thaw cycle is repeated 1-3 times to rupture the diatom cells, obtaining freeze-thawed diatoms. These are then disrupted using an ultrasonic disruptor to obtain diatoms containing biomass-based surfactants. This technical solution utilizes a combination of ultrasonic disruption and freeze-thaw treatment to efficiently destroy the diatom cell wall and improve the extraction efficiency of surfactant biomass.

The invention belongs to the technical field of oyster storage and processing, and provides a method for improving taste quality of oysters in a storage period by using vitamin B3, and the method comprises the following steps: mixing sea salt with water, and adding vitamin B3 to obtain artificial seawater for later use; the oysters are put into artificial seawater to be purified for 45-50 h, and purified oysters are obtained; the method comprises the following steps: pre-treating purified oysters in an environment of 8-12 DEG C for 1.5-2.5 hours, and preserving the oysters at 2-6 DEG C; according to the invention, only vitamin B3 is adopted to purify oysters, so that the reduction of nutritional ingredients can be delayed, and the loss speed of taste substances during storage can be slowed down.

The invention relates to a universe system for fish ecological adaptability training and a training method of the universe system. The fish universe system comprises a cuboid tank body with the top open, gravels are laid at the bottom in the tank body, artificial seawater is poured into the tank body, and the interior of the tank body is divided into a shoal area, an aquatic plant area, a partition area and a reef area in the length direction. The shoal area is used for simulating a shoal in a field water area, some small fishes which are not good at swimming tend to hide in a shoreside structure to obtain a shelter, and the movement of large predators can be limited by the shallow water body. The aquatic plant area is mainly composed of a structure of a plastic aquatic plant model, and the reef area is mainly composed of a medium-sized complex reef. The two areas are mainly used for providing habitats of different structure types for small fishes and correspond to different complex structure scenes in the field respectively. The partition area is used for separating the aquatic plant area from the reef area.

A formula, APSW (Anti Pathogen Seawater), for preparing seawater for fish farms and aquariums that contains some or all the normal ingredients of sea salt with the exception of having low or no sulfates. Testing by the applicant has revealed that sea water without sulfates eliminates amyloodiniosis (Amyloodinium ocellatum), one of the most serious diseases of warm water marine fish. By reducing or eliminating sulfate from seawater, the invention may also prevent or provide treatment for other ectoparasites on aquatic animals, treat noxious dinoflagellates, treat bacterial diseases of marine animals, slow the growth or reduce the levels of microbes or parasites in shellfish, “starve” pathogenic bacteria, treat water-borne infectious diseases, prevent the formation of “off flavor” and prevent the formation of hydrogen sulfide.

The application belongs to the technical field of super-hydrophilic coating, and particularly relates to a self-repairable super-hydrophilic coating, a preparation method and application thereof.The self-repairable super-hydrophilic coating comprises the following components in parts by weight: 10-20 parts of epoxy resin, 1-5 parts of curing agent, 10-150 parts of solvent, 0-7 parts of nano filler, 5-15 parts of modifier and a hydrophilic component dissolved in another solvent.The super-hydrophilic coating has excellent oil pollution prevention characteristics, and can have antibacterial, scale inhibition and drag reduction characteristics, and can be applied to the fields of oil adhesion prevention, microbial adhesion prevention, underwater drag reduction, oil-water separation and the like, can effectively resist conventional organic oil phase pollution, even high-viscosity crude oil pollution, and can be repaired in water, strong acid, strong base, high salt and artificial seawater, solving the problem that the existing super-hydrophilic coating cannot be repaired in an extreme underwater environment.

This invention belongs to the field of ecohydraulics, and specifically relates to a sliding dike device for an artificial seawater lake and its usage method. The device includes a dike, a lifting device, a buffer assembly, a replaceable aquaculture tank, a sliding base, and a detachable base. The lifting device is mounted on the dike and controls the lifting and lowering of the sliding base via a first motor and a second motor. A buffer assembly is located around the sliding base. The detachable base is connected above the sliding base, and a replaceable aquaculture tank is connected above the detachable base. This invention, through the lifting device, sliding base, and detachable base, allows the height of the aquaculture tank to change with the water level of the artificial lake. Furthermore, the aquaculture tank and sliding base are easily detachable, and an internal oxygenation device ensures the survival of the organisms inside the aquaculture tank.

The invention discloses a reef limestone lithification test device and a use method thereof. The reef limestone lithification test device comprises an injection assembly, a lithification test assembly, a collection assembly and a monitoring assembly. According to the device, CO2 provided by a CO2 low-temperature pump and artificial seawater provided by a seawater low-temperature pump are mixed in a low-temperature chamber to form CO2 seawater solutions with different concentrations, and then the CO2 seawater solutions are sprayed on coral debris samples to simulate the corrosion effect of acid rainwater on the coral island reef surface layer; the lithiation test box can also provide underground water dynamic conditions and different temperature and vertical pressure conditions for the coral clastic sample to simulate the natural diagenesis process of the reef limestone, and the spectrometer can monitor the volume change of calcite components in the coral clastic sample and the porosity change of the coral clastic sample in real time. The device provided by the invention can provide complex geological conditions required by diagenesis of the reef limestone, accelerates and simulates a corrosion process and a cementation process of the reef limestone from sand to rock in a laboratory scale, and provides technical guidance and a scheme for researching water-rock interaction of the reef limestone.

This application relates to an integrated micro energy recovery module, recovery method, and its artificial seawater desalination system, applicable to portable seawater desalination equipment requiring only artificial power supply, low flow rate, high pressure. The integrated micro energy recovery module includes a plunger pump assembly, a check valve assembly, and a hydraulic directional valve. The energy recovery module includes: ① a set of dual plunger pumps, where in each operating cycle, one pump discharges high-pressure raw water while the other pump simultaneously draws water, shortening the system's output liquid pressure pulsation cycle; ② a directional valve that automatically switches direction using hydraulic energy; ③ a directional valve used to switch the operating states of the two plunger pumps, allowing them to alternate between suction and discharge states; ④ through the directional valve, high-pressure concentrated water is injected into the back side of the plunger, utilizing residual liquid pressure to propel the piston. The system's energy recovery efficiency can reach over 90%.

The utility model discloses an anti -fouling paint copper ion exuding rate testing arrangement, including constant temperature water tank, set up in the storage tank and test container of constant temperature water tank, first slide rail subassembly, be located constant temperature water tank top, along first direction extension setting, second slide rail subassembly, through the connecting plate sliding connection in first slide rail subassembly, second slide rail subassembly along second direction extension setting, can along first slide rail subassembly along first direction remove, test drum, through the fixed frame connection in second slide rail subassembly, can along second slide rail subassembly along second direction remove, and test drum can rotate around second direction relative to fixed frame, filter circulating pump, through the connection pipe with storage tank inside intercommunication, to purify the artificial seawater in storage tank, the surface of test drum is coated with the anti -fouling paint of waiting and testing, and the storage tank is used for soaking test drum, and the test container is used for accommodating test drum and supplies test drum to rotate in its inside, to get the exudation liquid sample.

The invention belongs to the technical field of deep macro organism culture, and particularly discloses a deep macro organism culture system and method.The deep macro organism culture system comprises a culture kettle, a seawater supply unit and an isobaric food supply mechanism, and the seawater supply unit comprises a one-way needle valve, a high-pressure seawater cooling cabin and an artificial seawater tank; the artificial seawater tank, the booster pump, the high-pressure seawater cooling cabin, the one-way needle valve and the culture kettle are sequentially connected. The isobaric food supply mechanism comprises a second high-pressure ball valve, a third high-pressure ball valve, a nutrient solution injection mechanism and an overflow valve; the nutrient solution injection mechanism, the third high-pressure ball valve and the second high-pressure ball valve are sequentially connected to the outlet end of the one-way needle valve. The overflow valve is connected to the outlet end of the third high-pressure ball valve. On the premise that seawater in the main pipeline continuously flows, the nutrient solution is injected into the system, and the technical problem of feeding under the conditions that a pump is not stopped and the pressure and flow stability of the system are not damaged is solved through the mode.

This invention relates to a test apparatus for simulating wet-dry cycle corrosion of steel components for offshore wind turbine foundations. A support rod is installed at the top of the test tank, and nylon ropes are connected to the support rods and lowered into the test tank. Multiple epoxy magnets are vertically spaced and tied to the nylon ropes to attract the steel components. A first submersible pump is installed at the bottom of the test tank, and its outlet connects to a storage tank via a first drain pipe. The storage tank contains artificial seawater, and a second submersible pump is installed at its bottom, with its outlet connecting to the test tank via a second drain pipe. This invention simulates the wet-dry cycle corrosion process of steel components for offshore wind turbine foundations in a marine environment by circulating artificial seawater between the test tank and the storage tank. Furthermore, it allows for simultaneous corrosion tests of steel components under multiple sets of different wet-dry time ratios, providing a simple and reliable technical means for studying the influence mechanism of wet-dry time ratio on the corrosion behavior of steel components.

The application provides a seawater nitrate measurement method based on a spectral fingerprint, which comprises the following steps: step one, preparing an artificial seawater solution and constructing a spectral fingerprint S1 of the artificial seawater solution; step two, constructing spectral fingerprints S2, S3, S4 and S5 of organic matter-artificial seawater solutions and a nitrate-artificial seawater solution; step three, establishing a spectral reconstruction equation; step four, establishing a multivariate regression equation of a nitrate concentration C; step five, substituting an absorption spectrum of seawater into the spectral reconstruction equation in step three to obtain fitting parameters, and finally substituting the fitting parameters into the multivariate regression equation in step four to obtain the nitrate concentration of the seawater. The method effectively suppresses the interference of ions, organic matters and the like in seawater on the spectrum of nitrate, improves the measurement precision of nitrate in high-salinity water samples, improves the adaptability of a spectral analysis instrument to a marine environment, realizes rapid calibration, and improves the spectral model migration capability.

The invention discloses an RTM forming composite material surface micropore filling method. The method comprises the following steps that S1, the surface of a composite material is pretreated; s2, selecting low-viscosity epoxy resin as a filling agent, uniformly coating the surface of the treated composite material with the low-viscosity epoxy resin, and placing the coated composite material in a vacuum bag for vacuumizing; s3, after the filling agent permeates, gradient heating is adopted to enable the filling agent to be cured to form a micropore sealing layer; s4, the cured micropore sealing layer is polished and cleaned; s5, low-concentration protective colored paint is sprayed on the surface of the cleaned micropore sealing layer, the micropore sealing layer is placed in normal-temperature artificial seawater to be soaked, paint surface defects are detected after soaking, and filling is conducted; and S6, high-concentration protective colored paint is sprayed on the surface of the filled low-concentration protective colored paint, and a final protective surface layer is formed. The problem that in the prior art, the marine environment resistance is reduced due to micropores in the surface of an RTM forming composite material is solved.

The application relates to the field of paint testing, in particular to a test method for the cleaning resistance of marine antifouling coatings. The test method provides an experimental device, which comprises a sample plate for coating, a groove for accommodating the sample plate, a brush disc, a driving device for driving the brush disc to rotate and move up and down, and a control system electrically connected with the driving device. The test method can reliably evaluate the cleaning resistance of the antifouling coating through steps such as artificial seawater immersion experiment, underwater cleaning experiment, coating detection and coating loss grade evaluation. The application can efficiently and reliably evaluate the cleaning resistance of the antifouling coating in a short time in a room, can provide scientific data support for the development and research of the antifouling coating, and can shorten the period from the laboratory development of the antifouling coating system to the actual application.

The invention discloses a coral concrete self-healing agent based on microbial mineralization and application of the coral concrete self-healing agent. The self-healing agent comprises the following substances in parts by weight: 40-60 parts of artificial seawater, 120-160 parts of a gel material, 110-150 parts of coral sand, 50-80 parts of bacillus subtilis, 170-210 parts of coral reef and 0.5-3 parts of a water reducing agent. The self-healing agent is applied to coral concrete self-repairing. Under the seawater environment, the survival rate of microorganisms is high, mineralized products are high in stability, the repairing agent and the coral concrete interface are good in cohesiveness, the repairing time is short, durability is good, and the repairing agent can adapt to the seawater high-salt and high-alkali environment; bacillus subtilis has good alkali resistance, grows well when the pH value is 7-9, has good mineralization deposition induction capacity, and can convert a calcium source into calcium carbonate crystal precipitates with two crystal forms; the bacillus subtilis, the composite calcium source and the yeast extract have a synergistic effect, and the crack filling density is improved by 40%.