43 results about "Artificial seawater" patented technology

The utility model discloses a concrete corrosion resistance test device, which comprises a box body, a liquid inlet, a liquid outlet, a water inlet, a water outlet, a water outlet, a water inlet pipe, a water outlet pipe, a water inlet pipe and a water outlet pipe, and is characterized in that the box body is provided with an inner cavity; a bearing platform; the multiple concrete test block mounting structures correspond to the multiple pairs of longitudinal sliding grooves one to one, each concrete test block mounting structure comprises at least two supporting seats, and each supporting seat comprises a pair of supporting frames, a pair of sliding blocks, a pair of jacking transverse rods and a tensioning transverse rod; the stirring shaft is mounted at the inner bottom of the cover plate, and the stirring shaft is driven by a stirring motor to drive blades to rotate. The device disclosed by the utility model can simulate the impact of artificial seawater serving as a corrosive liquid on concrete, and can be used for carrying out corrosion resistance tests on a plurality of concrete test blocks, so that the quality of the concrete test blocks is detected.

The invention provides a method for incubating larvae of macrobrachium rosenbergii by utilizing underground well water, which comprises the following steps: removing calcium and magnesium ions in the underground well water by adopting a chemical precipitation method to prepare treated well water; mixing the treated well water with external river water to form basic seedling culture water; basic seedling culture water is added into the seedling culture pond, then quicklime and bleaching powder are added, and the basic seedling culture water is disinfected; artificial salt is added into the disinfected basic seedling culture water, and artificial seawater is prepared; larvae of macrobrachium rosenbergii are stocked in artificial seawater, and breeding production is carried out. According to the method, through systematic integration of cooperative utilization of underground well water and external river water, a seasonal dynamic disinfection strategy and multi-dimensional seedling raising environment accurate regulation and control, the incubation effect of macrobrachium rosenbergii larvae is remarkably improved, and the physicochemical indexes of water quality are better, the growth of the larvae is faster, the metamorphosis development is faster, and the survival rate is higher.

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The invention belongs to the technical field of gene intervention, and provides a feeding-mediated marine mussel gene silencing method which comprises the following steps: linearizing plasmids to obtain linearized plasmids; determining a cDNA fragment of the target gene from the target mussel, and performing amplification by using the specific primer pair to obtain a target gene fragment; connecting the target gene segment with the linearized plasmid to obtain a connection product; transforming the connection product into escherichia coli, screening to obtain a positive strain, extracting target recombinant plasmids, and transforming the target recombinant plasmids into escherichia coli for expressing dsRNA to obtain engineering bacteria; engineering bacteria and microalgae are mixed according to the cell number ratio of (90-110): (1-2), a bacteria-algae feeding mixture is obtained, and mussels are fed with artificial seawater containing the bacteria-algae feeding mixture for 1-10 d. The method disclosed by the invention does not cause antioxidant system disorder or energy metabolism abnormality, and is a gene silencing strategy with higher biological safety.

This invention discloses a method for removing filamentous algae infecting hydra, comprising the following steps: (1) preparing seawater containing copper ions with a concentration of 100-250 μg / L using artificial seawater as a solvent; (2) preparing food to cultivate hydra; (3) using the prepared copper-ion-containing seawater to cultivate hydra infected with filamentous algae to remove the filamentous algae, during which the copper-ion-containing seawater is replaced every 2-3 days to maintain the copper ion concentration, at which time the hydra will degenerate and form hydra dormant bodies; (4) after the filamentous algae are completely removed, the seawater is replaced with normal seawater, and the hydra population enters the recovery period; during the recovery period, the filamentous algae do not reappear, and new hydra grow from the hydra dormant bodies after 1-16 days, and the hydra subsequently return to normal. The method provided by this invention can not only effectively remove filamentous algae that infect hydra, but also allow the hydra to quickly recover into a healthy hydra colony in normal seawater after copper ion treatment, and continuously form and release healthy jellyfish.

The invention discloses a method for collecting and dyeing sperms of Chinese prawns, and belongs to the technical field of germplasm resource protection and utilization. The method comprises the following steps: grinding Penaeus chinensis spermatophore with outer membrane removed in a glass homogenizer, adding sterilized artificial seawater, grinding until the solution becomes milky white, and filtering to obtain sperm; and mixing the sperm with eosin-nigrosine dye liquor, and standing to obtain the dyed Chinese prawn sperm. The collecting and dyeing steps are simple and easy to operate, the whole process is short in time consumption, small in sperm damage, visual in effect and high in sperm survival rate. The staining method disclosed by the invention is not only suitable for staining fresh sperms, but also can be used for staining the thawed and resuscitated sperms after cryopreservation, the staining observation after thawing is clear, and the sperms are not easy to crystallize.

The invention relates to the technical field of sea squirt culture, and particularly discloses a full-artificial culture system for ciona intestinalis, a seawater preparation unit is used for purifying tap water and adding sea salt to adjust the salinity of simulated seawater; the culture unit comprises a culture pond, a seawater supply system and a drainage system; the culture pond is made of a transparent material, the seawater supply system conveys prepared seawater to the culture pond through a pipeline, and the drainage system is used for regularly replacing and discharging wastewater in the culture pond; the seawater circulating unit is used for purifying seawater in the culture system and promoting circulating flow of water in the culture system; the temperature control unit is used for controlling constant water temperature in the culture pond; the illumination control unit is used for simulating natural light to irradiate the sea squirts. By means of the artificial seawater circulation system, dependence on natural seawater is completely eliminated, and full-artificial breeding of ciona ciona is achieved. According to the system, internal circulation operation is achieved, waste of water resources is reduced, and meanwhile the risk of environmental pollution is reduced.

The invention discloses an early-strength, quick-setting concrete for emergency repair and construction of island and reef projects and a preparation method thereof. The method comprises weighing artificial seawater, sulphoaluminate cement, fly ash, silica fume, coral sand, regenerated coral coarse aggregate, a water reducer, a liquid alkali-free quick-setting agent and stainless steel fiber, uniformly mixing the sulphoaluminate cement, silica fume and fly ash to obtain a cementitious material, reserving a portion of artificial seawater, dry-stirring the cementitious material, then adding the remaining artificial seawater and the water reducer and stirring, then respectively adding coral sand, regenerated coral coarse aggregate and stainless steel fiber and stirring, adding the liquid alkali-free quick-setting agent and the reserved artificial seawater to the resulting mixture to obtain a concrete mixture in a gel state, placing the mixture into a mold for vibration, standing, demoulding and curing to obtain early-strength, quick-setting concrete. The early-strength, quick-setting concrete of the present invention has the advantages of short setting time, good early strength, high mechanical strength and stable chemical properties, and has good economic and environmental effects.

The utility model discloses a mariculture source water short-process treatment system, which comprises a box body, an immersed ultrafiltration membrane unit, a backwashing and medicine washing unit, a culture unit and a control unit, and the immersed ultrafiltration membrane unit, the backwashing and medicine washing unit and the culture unit are all integrated in the box body. The device is integrated in a box body, is high in integration level, takes the immersed ultrafiltration membrane assembly as a core, and is high in filtering precision and good in produced water quality; the immersed ultrafiltration membrane assembly can remove float sludge and harmful microorganisms in natural seawater, does not generate harmful by-products, does not bring harmful effects to an artificial mariculture system, and can improve the survival rate of cultured aquatic products.

The preparation method comprises the following steps: weighing artificial seawater, sulphoaluminate cement, fly ash, silica fume, coral sand, regenerated coral coarse aggregate, a water reducing agent, a liquid alkali-free accelerator and stainless steel fibers, uniformly mixing the sulphoaluminate cement, the silica fume and the fly ash, and uniformly stirring to obtain a mixture; a part of artificial seawater is reserved, the gelling material is subjected to dry stirring, then the remaining artificial seawater and a water reducing agent are added and stirred, coral sand, regenerated coral coarse aggregate and stainless steel fibers are added and stirred, a liquid alkali-free accelerator and the reserved artificial seawater are added into the obtained mixture, and the mixture is uniformly stirred. And putting the gel-state concrete mixture into a mold, vibrating, standing, demolding and curing to obtain the early-strength quick-setting concrete. The early-strength quick-setting concrete has the advantages of short setting time, good early strength, high mechanical strength, stable chemical properties and the like, and has good economic and environment-friendly effects.

The invention provides a method for efficiently separating and purifying SAR11 bacteria, belongs to the technical field of environmental microorganisms, and aims to solve the problems that the SAR11 bacteria are difficult to culture, are easily inhibited by infectious microbes and the like. The method comprises the following steps: pre-filtering a seawater sample by using a 0.45 m mixed cellulose ester film, and enriching SAR11; the method comprises the following steps: by taking artificial seawater as a matrix, adding a mixture of sodium pyruvate, methionine, glycine and vitamins with specific concentration to prepare a culture medium, inoculating a sample into a 24-pore plate, and culturing under the conditions of 17-26 DEG C and darkness / 3000 / 5000lx; the method comprises the following steps: dyeing a sample for 45 minutes by SYBR Green I, and monitoring according to SAR11 light scattering and nucleic acid characteristics by using a flow cytometer (488nm laser excitation); target cells are subjected to expanding culture to obtain single colonies, and 50% glycerol is used for low-temperature breed conservation. According to the method, the separation success rate is increased, the period is shortened, and a support is provided for researching the physiological and ecological functions and the carbon cycle effect of the SAR11.

The invention belongs to the technical field of abyssal macroorganism culture, and particularly discloses a abyssal macroorganism long-term culture system and method, and the abyssal macroorganism long-term culture system comprises a magnetic coupling type rodless seawater tank, a reaction kettle and an artificial seawater cabin. A magnetic coupling type rodless seawater cylinder comprises a cylinder body, a piston, an annular sliding block and a pressure sensor. The cylinder body is provided with a built-in cavity, and the piston is arranged in the built-in cavity in a sliding mode and divides the built-in cavity into a water inlet cavity and a water outlet cavity. The annular sliding block and the piston are magnetically coupled in a non-contact mode to form stable magnetic attraction force, the reaction kettle is provided with a water inlet end and a water outlet end, and the water inlet end, the first electromagnetic valve and the water inlet cavity are sequentially connected; the water outlet end, the second electromagnetic valve and the water outlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the third electromagnetic valve and the water inlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the fourth electromagnetic valve and the water outlet cavity are connected in sequence; according to the invention, isovolumetric replacement of new seawater and old seawater is realized, culture pressure is accurately stabilized, and biological physiological stress reaction can be effectively avoided.

The invention provides a trace element formula suitable for industrial recirculating aquaculture of penaeus vannamei under an artificial seawater condition and a use method of the trace element formula, and relates to the field of industrial recirculating aquaculture. The trace element formula comprises potassium bromide, boric acid, strontium chloride, sodium silicate, zinc sulfate, ferrous sulfate, copper sulfate, manganese chloride, sodium selenite, ammonium molybdate, calcium gluconate and lysine, and the trace elements are added into a common feed to feed penaeus vannamei boone. The defects in the prior art are overcome, the growth efficiency of the penaeus vannamei boone is effectively improved by supplementing related trace elements in the feed, and the economic benefits of culture are increased.

To provide a method for producing artificial seawater, capable of reducing labor of a breeder or a staff of an aquarium facility or the like, and capable of being prepared in a short time.SOLUTION: A method for producing artificial seawater according to an embodiment of the present invention includes a step of preparing near-saturated salt water, a step of diluting the near-saturated salt water, and a step of dissolving trace components other than NaCl in the near-saturated salt water, wherein the trace components include at least magnesium ions, calcium ions, potassium ions, sodium ions, chloride ions, bicarbonate ions, and sulfate ions.SELECTED DRAWING: Figure 1

The invention relates to a seawater concrete hydration process regulation and control method with a carbon sequestration effect, aiming at the problems of concentrated heat release, easy cracking and the like in early hydration of large-volume seawater concrete, the early hydration process is regulated and controlled by using CO2 in a mixing stage, the main hydration heat release peak is delayed by 0.5-3 hours, the peak value is reduced by 10-30%, the accumulated heat release in 72 hours is basically unchanged or slightly reduced, and the total hydration heat release is reduced by 10-30%. Therefore, the risks of thermal cracks and shrinkage cracks are reduced, and the carbon sequestration capacity is improved. The method comprises the following steps: adding a cementing material and aggregate into a closed reactor, and stirring until dry materials are uniformly mixed; air in the reactor is pumped out, and CO2 gas is injected into the reactor to reach preset air pressure; the method comprises the following steps: injecting seawater or artificial seawater into a reactor, uniformly mixing, enabling CO2 to be subjected to carbonation reaction with calcium and magnesium ions and hydration products in a system, and forming a compact shell layer on the surfaces of clinker particles and a slurry-aggregate interface by generated nano-scale CaCO3, calcium and magnesium carbonate and magnesium-containing calcium silicate hydrate; therefore, the main hydration heat release peak value of the seawater concrete is reduced and the peak value occurrence time is delayed.

The invention discloses slag-sulphoaluminate compound gelled seawater and sea sand mortar and a preparation method thereof, and relates to the technical field of building materials. Comprising the following component raw materials: 180-220 parts of functional aggregate; 100 parts of a cementing material; 40 to 50 parts of a functional mixing liquid; the functionalized aggregate is sea sand loaded with a nanocrystalline seed layer on the surface; the cementing material is prepared from granulated blast furnace slag powder, natural anhydrite and high belite sulphoaluminate cement clinker; the functionalized mixing liquid is seawater or artificial seawater dissolved with a composite thixotropic-flocculating agent. According to the scheme, the core problems of low strength and poor durability caused by interface looseness and erosion medium permeation of the seawater and sea sand concrete are solved; and full resource utilization of the seawater, the sea sand and the slag is realized, and high performance, high durability and remarkable economic benefits are achieved.

The invention relates to the technical field of sea grass bed cultivation, in particular to a sea grass seedling raising automatic monitoring device which comprises a fixing base used for connecting all components. The two brackets are symmetrically arranged at the two ends of the fixed seat; the monitoring mechanism is used for detecting the concentration of the artificial seawater; the adjusting mechanism is connected with the fixed seat and is used for adjusting the concentration of the artificial seawater; the adjusting mechanism comprises a movable seat and a movable seat, wherein the movable seat is in sliding connection with the fixed seat; the salt spreading mechanism is arranged in the moving seat and is used for intermittently spreading salt; and the moving mechanism is in transmission connection with the moving seat and is used for driving the moving seat to move. According to the device, independent artificial seawater salt supplementation can be automatically performed on each plant of the sea grass bed in a targeted manner under the condition that the seawater concentration is monitored in real time, manpower consumption is reduced, it is guaranteed that the growth environment of each plant of sea grass can recover to the specified concentration in the fastest time, the sea grass cultivation precision is improved, and the cultivation efficiency is also improved.

The present invention aims to provide a closed-loop land-based aquaculture method for fish that can improve investment efficiency and also improve the rate of nitrate removal. [Solution] A closed-circuit land-based aquaculture method for farming fish on land using seawater or artificial seawater, wherein the rearing water in the rearing tank is circulated through a circulation path, the rearing water is purified as it passes through the circulation path, and the rearing water temperature is controlled to 19°C or lower, and the closed-circuit land-based aquaculture method is characterized by forming a section in the upper layer of the rearing water in the rearing tank with a partition wall that prevents fish from entering, and cultivating Ulva prolifera in the section.

This invention discloses a method for extracting and applying biomass-based surfactants for dispersing oil spills, comprising the following steps: Step 1, diatom cultivation: *Phaeodactylum tricornutum* is inoculated into artificial seawater with a salinity of 30‰, F / 2 nutrient solution is added, and it is cultured in a constant temperature and light incubator to obtain *Phaeodactylum tricornutum* in a stable concentration phase; Step 2, freeze-thaw-ultrasonic disruption: The *Phaeodactylum tricornutum* obtained in Step 1 is frozen in a refrigerator, then thawed in a water bath, and the freeze-thaw cycle is repeated 1-3 times to rupture the diatom cells, obtaining freeze-thawed diatoms. These are then disrupted using an ultrasonic disruptor to obtain diatoms containing biomass-based surfactants. This technical solution utilizes a combination of ultrasonic disruption and freeze-thaw treatment to efficiently destroy the diatom cell wall and improve the extraction efficiency of surfactant biomass.

The invention belongs to the technical field of oyster storage and processing, and provides a method for improving taste quality of oysters in a storage period by using vitamin B3, and the method comprises the following steps: mixing sea salt with water, and adding vitamin B3 to obtain artificial seawater for later use; the oysters are put into artificial seawater to be purified for 45-50 h, and purified oysters are obtained; the method comprises the following steps: pre-treating purified oysters in an environment of 8-12 DEG C for 1.5-2.5 hours, and preserving the oysters at 2-6 DEG C; according to the invention, only vitamin B3 is adopted to purify oysters, so that the reduction of nutritional ingredients can be delayed, and the loss speed of taste substances during storage can be slowed down.

The invention relates to a universe system for fish ecological adaptability training and a training method of the universe system. The fish universe system comprises a cuboid tank body with the top open, gravels are laid at the bottom in the tank body, artificial seawater is poured into the tank body, and the interior of the tank body is divided into a shoal area, an aquatic plant area, a partition area and a reef area in the length direction. The shoal area is used for simulating a shoal in a field water area, some small fishes which are not good at swimming tend to hide in a shoreside structure to obtain a shelter, and the movement of large predators can be limited by the shallow water body. The aquatic plant area is mainly composed of a structure of a plastic aquatic plant model, and the reef area is mainly composed of a medium-sized complex reef. The two areas are mainly used for providing habitats of different structure types for small fishes and correspond to different complex structure scenes in the field respectively. The partition area is used for separating the aquatic plant area from the reef area.

The present invention belongs to the field of copper alloy technology, specifically relating to a corrosion-resistant white copper alloy containing a high proportion of special grain boundaries and a preparation method thereof. The corrosion-resistant white copper alloy comprises the following elements, measured by mass percentage: 9-12% Ni; 1.5-1.9% Fe; 0.6-1% Mn; 0.18-0.4% Cr; 0.03-0.05% Ce; the remainder being Cu; the mass ratio of Ni to Cr is 30-50:1; and the number of special grain boundaries in the corrosion-resistant white copper alloy is greater than 55%. Under room temperature conditions, the corrosion-resistant white copper alloy provided by the present invention has a tensile strength greater than 350 MPa, an elongation greater than 40%, a static full immersion uniform corrosion rate less than 0.015 mm / a, and a uniform corrosion rate ≤0.04 mm / a under 3 m / s artificial seawater scouring conditions. Compared with traditional B10 white copper alloy, the white copper alloy provided by the present invention has better mechanical properties and corrosion resistance.

A formula, APSW (Anti Pathogen Seawater), for preparing seawater for fish farms and aquariums that contains some or all the normal ingredients of sea salt with the exception of having low or no sulfates. Testing by the applicant has revealed that sea water without sulfates eliminates amyloodiniosis (Amyloodinium ocellatum), one of the most serious diseases of warm water marine fish. By reducing or eliminating sulfate from seawater, the invention may also prevent or provide treatment for other ectoparasites on aquatic animals, treat noxious dinoflagellates, treat bacterial diseases of marine animals, slow the growth or reduce the levels of microbes or parasites in shellfish, “starve” pathogenic bacteria, treat water-borne infectious diseases, prevent the formation of “off flavor” and prevent the formation of hydrogen sulfide.

The invention relates to the field of cosmetics, in particular to deep sea fish skin collagen peptide, a preparation method thereof and cosmetics. Drying the fat-removed deep sea fish skin to obtain dried deep sea fish skin; mixing the first fermentation seed solution and the second fermentation seed solution to obtain a mixed fermentation seed solution; inoculating the mixed fermentation seed solution into a fermentation culture medium for fermentation; the first fermentation seed solution is a pseudoalteromonas gelatinolyticum seed solution; the second fermentation seed solution is a lactobacillus plantarum seed solution; based on the total volume of the fermentation culture medium, the fermentation culture medium is prepared from 20.0-100.0 g / L of dried deep sea fish skin and 0.5-1.5 g / L of sodium carbonate; 0.5 to 1.5 g / L of disodium hydrogen phosphate; 0.1 to 0.5 g / L of monopotassium phosphate; a solvent of the fermentation culture medium is artificial seawater. The deep sea fish skin collagen peptide has a stable collagen structure, is rich in unique collagen functional peptide fragments, and can be applied to the field of cosmetics.

The present invention provides a method for constructing a seawater drowning-induced acute lung injury model and its application. When constructing the model, both pulmonary microvascular endothelial cells and type II alveolar epithelial cells are inoculated onto a transwell insert, the transwell insert is placed in a lung chip, and the lung chip is placed on a shaker for dynamic culture for 5 days to form a complete air-liquid interface, thus completing the construction of the lung chip; the lower channel of the lung chip is replaced with 1 mL of fresh mixed culture medium, and 500 μL of artificial seawater is slowly added to each of the two holes in the upper channel, and then it is placed on a shaker in an incubator at 37°C with 5% carbon dioxide for dynamic culture for 2 hours. The present invention applies the lung chip technology to the seawater drowning-induced acute lung injury model, and at the same time uses pulmonary microvascular endothelial cells and type II alveolar epithelial cells. The seawater is directly added to the prepared lung chip and then slowly penetrates into the lower channel. The model is closer to the real occurrence process of seawater drowning and has more clinical value in the related research of seawater drowning-induced acute lung injury.

The application belongs to the technical field of super-hydrophilic coating, and particularly relates to a self-repairable super-hydrophilic coating, a preparation method and application thereof.The self-repairable super-hydrophilic coating comprises the following components in parts by weight: 10-20 parts of epoxy resin, 1-5 parts of curing agent, 10-150 parts of solvent, 0-7 parts of nano filler, 5-15 parts of modifier and a hydrophilic component dissolved in another solvent.The super-hydrophilic coating has excellent oil pollution prevention characteristics, and can have antibacterial, scale inhibition and drag reduction characteristics, and can be applied to the fields of oil adhesion prevention, microbial adhesion prevention, underwater drag reduction, oil-water separation and the like, can effectively resist conventional organic oil phase pollution, even high-viscosity crude oil pollution, and can be repaired in water, strong acid, strong base, high salt and artificial seawater, solving the problem that the existing super-hydrophilic coating cannot be repaired in an extreme underwater environment.

This invention belongs to the field of ecohydraulics, and specifically relates to a sliding dike device for an artificial seawater lake and its usage method. The device includes a dike, a lifting device, a buffer assembly, a replaceable aquaculture tank, a sliding base, and a detachable base. The lifting device is mounted on the dike and controls the lifting and lowering of the sliding base via a first motor and a second motor. A buffer assembly is located around the sliding base. The detachable base is connected above the sliding base, and a replaceable aquaculture tank is connected above the detachable base. This invention, through the lifting device, sliding base, and detachable base, allows the height of the aquaculture tank to change with the water level of the artificial lake. Furthermore, the aquaculture tank and sliding base are easily detachable, and an internal oxygenation device ensures the survival of the organisms inside the aquaculture tank.

The invention discloses a reef limestone lithification test device and a use method thereof. The reef limestone lithification test device comprises an injection assembly, a lithification test assembly, a collection assembly and a monitoring assembly. According to the device, CO2 provided by a CO2 low-temperature pump and artificial seawater provided by a seawater low-temperature pump are mixed in a low-temperature chamber to form CO2 seawater solutions with different concentrations, and then the CO2 seawater solutions are sprayed on coral debris samples to simulate the corrosion effect of acid rainwater on the coral island reef surface layer; the lithiation test box can also provide underground water dynamic conditions and different temperature and vertical pressure conditions for the coral clastic sample to simulate the natural diagenesis process of the reef limestone, and the spectrometer can monitor the volume change of calcite components in the coral clastic sample and the porosity change of the coral clastic sample in real time. The device provided by the invention can provide complex geological conditions required by diagenesis of the reef limestone, accelerates and simulates a corrosion process and a cementation process of the reef limestone from sand to rock in a laboratory scale, and provides technical guidance and a scheme for researching water-rock interaction of the reef limestone.

The preparation method comprises the following steps: (1) performing detoxification treatment on brine: performing multi-stage filtration and electrochemical detoxification treatment on preserved szechuan pickle pickling brine with the salinity of 15-25% (wt%) to obtain detoxified brine; (2) microbial agent activation treatment: inoculating bacillus subtilis into the detoxified brine with the inoculation amount of 0.8-1.2 kg / ton of brine, and performing aeration culture for 20-28 hours under the conditions that the temperature is 30 + / -2 DEG C and the dissolved oxygen is greater than or equal to 5mg / L to prepare activated brine; (3) dynamic regulation and control of salinity: mixing the obtained activated brine and artificial seawater according to a volume ratio of 1: 10-1: 30, and regulating the salinity of the mixed water body to 25 per thousand to 35 per thousand (wt%) through a PID (Proportion Integration Differentiation) control algorithm; (4) biological synergistic treatment: inoculating a complex microbial inoculant into the mixed water body after salinity regulation, and culturing at 25-30 DEG C for 36-48 hours to obtain culture brine; compared with the prior art, the preserved szechuan pickle pickling brine is efficiently converted, and the cost of mariculture is reduced when the preserved szechuan pickle pickling brine is applied to mariculture.

The invention belongs to the technical field of ecological restoration, and particularly relates to an epoxy resin-based mixture suitable for crab hole pouring and a preparation method thereof. The epoxy resin-based mixture can be efficiently cured within 12 minutes no matter in tap water or artificial seawater, has flowability and water sinking property, can be cured underwater, and is suitable for crab hole pouring.