127 results about "Artificial seawater" patented technology

The invention discloses a quick germination method of grass wrack seeds. The method comprises the following steps of: after the grass wrack seeds are washed clean, directly putting the grass wrack seeds into 0.5 percent low-salinity artificial seawater; putting the 0.5 percent low-salinity artificial seawater with the soaked grass wrack seeds into an incubator in the presence of constant temperature and illumination for culturing for 3 weeks to ensure that the grass wrack seeds germinate, wherein the holding temperature in the presence of the constant temperature and illumination is 12-16 DEG C, the illumination intensity is 40 mu molm<-2>s<-1> and the ratio of L:D is 12:12; leading the germinated seeds into a salinity raising and domestication stage: ensuring that the salinity of the artificial seawater for soaking the germinated grass wrack seeds is improved by 0.5 percent per day until reaching a normal seawater salinity of 3 percent; and then sowing the germinated grass wrack seedlings adapting to the natural seawater salinity in an appointed sea field. The method can ensure that the grass wrack seeds germinate quickly and completely and normally grow so that the probability of suffering from being swallowed by seed-eating organisms of the grass wrack seeds is greatly reduced and is beneficial to the recovery and reconstruction of sea grass meadows so as to quickly recover good marine ecological environment.

The invention discloses a subsurface-flow water supply culture method of Babylonia, belonging to the technical field of aquiculture. The method comprises the following steps of: (A) establishing a subsurface-flow culture pond of the Babylonia; (B) spreading Babylonia larvae on a supporting nozzle plate for culturing, and spreading a layer of sand grains on the Babylonia larvae layer; (C) enabling natural sea water or artificial sea water to flow into the culture pond through a water inlet pipe from the lower part of a culture medium; and (D) refreshing the natural sea water or the artificial sea water in the culture pond once through the water inlet pipe and a water outlet pipe every 3-7 days until the culture period of the Babylonia finishes. The invention also discloses a subsurface-flow water supply culture device of the Babylonia. The culture method of the invention does not depend on microorganisms to purify the culture environment but directly purifies and controls the culture environment through water flow and air inflation. In the culture process, sand grains of which the average diameter is 2mm-10mm are selected, thereby greatly enhancing the water flow permeability of the sand layer and effectively reducing the occurrence of Babylonia diseases.

The invention relates to a composite nutrition enhancer and a preparation method thereof, relating to a feed additive. The invention provides the composite nutrition enhancer, which not only contains large amount of DHA, ARA and natural yeast astaxanthin, but also contains multiple Omega 3 unsaturated fatty acids, multiple amino acids and trace elements. The composite nutrition enhancer is formedby composition of fission chytrid microalgae and phaffia rhodozyma with the weight ratio of 50 percent to 80 percent and 20 percent to 50 percent. All the components are weighed and mixed according to matching ratio after being crushed, mixed evenly, powder-sieved till the particle size is 8-25mum, inspected, weighed and packaged. The invention is especially applicable to feeding of live eels fryand shrimp postlarvae, artificial seawater seedling enhanced artemia salina sinnaeus, rotifer and cladocera (micrura), copepods and the like and various egg-laying poultries.

A modified epoxy resin ceramic coating against seawater and its production are disclosed. The ceramic coating consists of E52 epoxy resin, Neufokale epoxy resin, mixed solvent, composite accessory, A liquid with activated silicon powder with different grain size, mullite power and alpha-crystal-type alumina mixed ceramic powder and B liquid, which is prepared by reacting alicyclic ammonia with anacardol methanal to obtain phenol aldehyde ammonia curing agent with reactive hydrogen account 76-78. A liquid is mixed with B liquid. It's stable, has excellent adhesion, abrasive and heat resistances. It can resist 100 degree saturated sodium chloride, bitter halogen or artificial seawater erosion. It can be used for marine I-shaped steel architecture, seawater container and internal-external anti-corrosive coating of seawater transfer pipeline.

The invention discloses an intensified high-efficient cultivating method of prawns. The cultivating method comprises the following steps: preparation of a prawn pond: basking and disinfecting the pond, eliminating wild fishes, filling water and cultivating water; desalting prawn seeds: selecting health prawn seed with complete appendage, clean surface and appendage, normal figure, tidy specification, good wall attaching behavior and activity, and length of 0.4-0.6 cm; placing seeds in a desalting box, and configuring artificial seawater in the box body; putting the prawn seeds in the prawn pond; performing the early-period cultivation in the 1st- 20 days, feeding mixed feed twice per day; water quality control: feeding microbial growth regulating agent at intervals of 10-15 days at the middle and post periods of cultivation, wherein the feeding amount is 1.5-2.5 g per cubic meter, and the microbial growth regulating agent is used for removing harmful matter ammonia gas NH3 and nitrogen dioxide NO2 in the water; biological prevention and control: harvesting prawns, and fishing the prawns by a trawl, and a prawn cage in the morning; for the prawns cannot feed at 18 DEG C below, or stops growth namely; the prawns are died when water temperature is 7 DEG C; therefore, prawns must be marked before the end of October.

The invention discloses temperature-control fluid flushing analog experiment equipment in the material property test technique domain, which comprises the following parts: motor, stepless variable device, rotary drum with blade, vertical specimen rack around drum, storage container and thermostatic device, wherein the natural seawater or artificial seawater is injected in the thermostatic device to start pump when the temperature reaches the request; the motor is opened and the drum blade begins to rotate; the moving fluid makes the front-back face of connection the sample on the blade and sample around the drum do flushing operation. The invention can analog ship and sea structure material as well as protective coater against seawater flushing, which is convenient and rapid to estimate the seawater-proof flushing property of new sea material.

The invention discloses a pot culture process method of a mangrove plant, comprising the steps as follows: a double-layer flowerpot consisting of a transparent externally sleeved flowerpot and a built-in flowerpot is created, tidal flat soil is filled in the built-in flowerpot as growing base, the mangrove plant seedling or the intact embryo is planted in the growing base, the artificial seawater is filled in the flowerpot for culturing the mangrove seedling or the embryo, the artificial seawater is filled in and discharged from the flowerpot per day for culture management of the planted mangrove plant pot by simulating the ebb and flow law of the semi-diurnal tides, and aerating roots are guided and shaped into the required appearance when the mangrove plant grows and forms well-developed aerating roots in the externally sleeved flowerpot. The pot culture process method successfully transplants the mangrove plant into the room for culture and develops into the pot art, combines the indoor air purifying functions with the aesthetic values of the mangrove plant and plays an important role in creating a healthy and comfortable lifting environment for people.

The invention discloses a seawater spirulina selenium-rich culture method. The method comprises the following steps of: selecting one or combination of more of the seawater-naturalized spirulina platensis, spirulina maxima and microcystis aeruginos as the algae seed; based on the natural seawater, artificial seawater or the mixture of the two at any ratio, adding a selenium element with concentration of 1-160mg/L to serve as a culture medium; inoculating the algae seed into a photobioreactor containing the culture medium for culture; and then collecting the cultured spirulina body to obtain the seawater spirulina which is the selenium-rich seawater spirulina. Through the invention, the method is simple, the selenium addition concentration is low, the culture medium can be recycled for a long time, the operation is simple and convenient, the selenium enrichment ability is strong, the cost is low, the method is green and safe, etc. The bioactive substance of the seawater spirulina generates a synergistic effect with organic selenium in the algae body to realize the function, and can be applied to various diseases caused by selenium lack and oxidative damage of oxygen free radical for the selenium supplement additive, oxidation resistance, aging resistance, tumor resistance, Keshan disease prevention and treatment and the like.

The invention discloses a method for cyclically cultivating Epinephelus coioides with artificial seawater indoors, which comprises the following steps of preparation of artificial seawater, preparation of cultivation water, selection of fry, regulation and control of water quality, selection of feed, daily sewage suction and water change, and cleaness maintenance of the bottom and walls of a pond. The method is efficient and environment-friendly; Epinephelus coioides obtained according to the method can be high in quality and yield, and healthy, in addition the cultivating survival rate is high, and the feed coefficient is low.

The invention relates to a rapid test method of toxicity in marine oil spill organisms. The method takes photobacteria as tested organisms for testing the toxicity in the marine oil spill organisms and simultaneously utilizes artificial seawater for preparing water accommodation friction (WAF), thus avoiding the experiment error caused by the reduction of the salinity of marine photobacteria and the changes of living conditions of the photobacteria due to the application of pure water as solvent for preparing the WAF in previous photobacteria experiments.

The invention discloses a pesticide biological activity determination method which comprises the following steps that: (1) a sample (pesticide raw medicine or plant extract) is prepared into 10g/L mother solution by distilled water, the sample which is insoluble in water is first added with a small amount of acetone to be dissolved, and is gradually diluted by artificial seawater to the sample concentration; (2) 10mu L of artemia liquid is shifted to a 24-hole culture plate, and then 1.5ml sample liquid pesticide to be tested is added in to be put into a 25DEG C light incubator to be cultured, and no bait needs to be fed during the test; each treatment is repeated for four times, and simultaneously, the artificial seawater which contains a certain amount of acetone is set for reference; and (3) after 48h of culture, the number of dead artemia nauplii is observed under a anatomical lens, the corrected mortality is calculated, and the insecticidal biological activity of a sample to be tested is evaluated. The method provided by the invention has the advantages of sensitivity to the activity of most pesticide, wide sources, easy largely feeding, low determination cost, simple and convenient method and trace amount of required sample, and is particularly applicable to the screening or the determination of the trace amount of sample.

A method in which an aqueous water-soluble compound solution containing at least one impurity in a slight amount is passed through a column packed with an adsorbent onto which the impurity is selectively adsorbed to thereby remove the impurity from the aqueous solution, wherein an abnormal phenomenon in adsorption chromatography is caused to obtain an elute fraction having a higher concentration of the trace impurity than the raw solution and this fraction is removed. Thus, the trace impurity can be efficiently removed and the water-soluble compound can be obtained in a high-purity state. Applying this method to aqueous sodium chloride solutions gives sodium chloride crystals reduced in impurity concentration. Dissolving such sodium chloride crystals, which have a low potassium ion concentration, in water provides an aqueous sodium chloride solution for medical use. Also provided is a sodium chloride composition for preparing an artificial seawater for use in alga cultivation which is reduced in magnesium ion or calcium ion concentration.

The invention discloses a floating breakwater structure which is characterized by comprising a plurality of grid units. Each grid unit comprises transverse pipes, longitudinal pipes and three-way pipes, the transverse pipes and the longitudinal pipes are arranged uniformly and are connected one another by the three-way pipes so that one grid unit is formed. A plurality of triangular supports are connected between two rows of transverse pipes in the middle of the grid units through the three-way pipes, and breakwater nets are laid on two sides of the triangular supports. The transverse pipes or the longitudinal pipes at the boundary of the grid units are movably connected by a plurality of double-ring sleeves. The floating breakwater structure has remarkable wave breaking effects, is low-cost relatively, convenient to demount, can drain water flow, has small affection to natural conditions of ports, and can be used for protecting fish culture and enhancement facilities and water areas such as artificial seawater baths, and accordingly double effects of saving investment and keeping good water quality exchange are achieved.

The invention relates to the technical field of hydranth breeding in a laboratory, and provides a microscopic breeding method for aurelia aurita hydranth in a laboratory. The microscopic breeding method comprises seven steps of artificial seawater preparation, bait preparation, aurelia aurita hydranth transfer, aurelia aurita hydranth attachment, aurelia aurita hydranth feeding, artificial seawater replacement and subculture. According to the method, a six-hole culture plate or a single culture dish is selected as a culture utensil, the number of aurelia aurita hydranths is controllable and countable, and operations such as feeding and water replacement are convenient and easy to reach the standard; meanwhile, a biochemical incubator with higher control precision is selected as a culture instrument, on one hand, the problem that a current laboratory breeding water tank cannot reach the standard of experimental animal breeding in the aspects of constant temperature control, accurate quantitative feeding, water quality purification and the like is solved; and on the other hand, hydranth quantification and subculture are easy, the vitality of the hydranths is improved, and furthermore, the problem that a traditional breeding mode cannot carry out accurate quantitative scientific research experiments related to the hydranths is solved.

The invention discloses a culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof, belonging to the technical field of bacteria culture. The culture medium comprises the following components and contents: 3.64g/L of corn starch, 6.0g/L of tryptone, 6.29g/L of calcium carbonate, 4.0g/L of soya peptone, 0.10g/L of aminothiopropionic acid and 0.02g/L of tryptophan, wherein a solvent is artificial seawater. The invention cannot only meet requirement on shake flask experiment, but also can meet requirement on massively preparing Bacillamide C through fermentation of a fermentation tank.

The invention relates to a water estrogen pollution detection method based on oryzias melastigma vitellogenin, and belongs to the field of environmental pollution detection. Currently, no marine fish is used for environment estrogen biological detection, and biological detection technology of seawater estrogen pollution is insufficient. According to the invention, euryhalic male oryzias melastigma with strong adaptability is selected as an in-vivo model; a primer is designed; the expression of vitellogenin (VTG1) is detected by a real-time quantitative PCR method; and 18S ribosome RNA (18S) is used as reference genes to establish a detection system of the VTG1 transcriptional level. Male fish is exposed to 17-beta-estradiol and clean artificial seawater, which are used as a positive and a negative control of the system, and appropriate exposure time is explored. The detection method can be used for detection of environment estrogen pollution degrees of seawater samples, river estuary samples, and saltwater internal lake water samples.

The present invention relates to artificial seawater and its use to treat a variety of skin conditions, when that sea water has more magnesium in it than in naturally occurring seawater. The treatment of acne would healing and the like can be improved with the product of the present invention and with treatments thereof.

Solid sea water may be dissolved and diluted for transporting and culturing marine animal and plant, in building artificial sea water bathing beach, etc. The production process of solid sea water includes concentrating sea water or brine water to crystallize sodium chloride; freezing the residue brine water naturally in winter to produce mixed crystalline sodium sulfate salt; further concentrating the residual brine water in a brine water storage to obtain solid mixed slat; testing the products in the previous steps, crushing, and compounding solid sea water according to the components of sea water. The present invention has low production cost and can recover sea water resource and protect coastal ecological environment.

The invention discloses a method for evaluating environmental embrittlement resistance under a simulated condition of an R6 mooring chain. The method comprises the following steps of: step 1, determining a sampling site, wherein the two sets of samples are taken from the ring straight body of the R6 mooring chain; step 2, preparing a reference electrode and an anode electrode; step 3, mounting a set of samples on an electro-hydraulic servo universal material testing machine, placing the reference electrode and the anode electrode, and putting into the pre-configured artificial seawater to immerse a tensile sample and the electrodes; step 4, charging a CT sample with hydrogen, wherein charging time is 48 hours; step 5, setting a strain rate and starting the test; and step 6: evaluating crack resistance by a section shrinkage rate by a slow strain rate tensile test, and evaluating environmental sensitivity of a product by a KIEAC form by a compact tensile test. The method for evaluatingthe environmental embrittlement resistance under the simulated condition of the R6 mooring chain has simple operation and is suitable for the R6 mooring chain with tensile strength above 1100 MPa, andcan analyze the hydrogen evolution potential, thereby limiting the potential protected by a hull cathode.

The invention discloses seawater and coral sand mixed red mud-coal gangue based geopolymer concrete. The concrete comprises red mud, coal gangue, coral sand, gravel, a sodium hydroxide solution, a water glass solution, artificial seawater and a water reducing agent. The preparation method comprises the following steps: preparing artificial seawater; screening out fine sand from the coral sand; knocking large coal gangue blocks into small coal gangue blocks, and crushing the small coal gangue blocks; air-drying or drying the red mud slurry until the water content is lower than 3%, measuring thepH value of the red mud slurry, and then crushing the red mud slurry; preparing a red mud-coal gangue mixed raw material; preparing a sodium hydroxide solution; lowering the mold of the water glass through a sodium hydroxide solution; mixing the sodium hydroxide solution with the water glass solution subjected to mold reduction to form an alkaline activator; designing the total proportion of thematerials; and preparing all the prepared raw materials into a finished product. The prepared concrete is high in compressive strength and slump, low in cost and wide in application range, recycling of two large solid wastes is achieved, and a new building material source is provided for island and reef construction.

The invention provides a slow tensile testing device for an R6 grade mooring chain under cathodic protection, comprising a barrel-shaped container filled with artificial seawater and a cover plate. The center of the cover plate is provided with a through hole a for placing an upper stretching jig, the left side of the through hole a is provided with a through hole b for mounting an auxiliary anode, and the right side of the through hole a is provided with a through hole c for mounting a reference electrode. One end of a power output device is connected to the auxiliary anode, and the other endis connected to the upper stretching jig. One end of a voltage test device is connected to the reference electrode, and the other end is connected to the upper stretching jig. A stretching sample issandwiched between the upper stretching jig and a lower stretching jig, and a sealing device is disposed between the lower stretching jig and the bottom of the barrel-shaped container. According to the slow tensile testing device for the R6 grade mooring chain under the cathodic protection, the electrochemical parameter measurement and the measurement environment control can be simultaneously realized without affecting experiment, the environment of cathodic protection in seawater is simulated, the slow strain rate tensile test of R6 grade mooring chain is accurately completed to realize the comprehensive investigation of environmental embrittlement resistance thereof.

The invention relates to a device for preparing a suspended natural gas hydrate and a preparation method thereof. The device comprises a low-temperature circulating freezing device, a microwave heating device and a hydrate reaction kettle. The preparation method comprises the following steps of: coating a water repellent on a skeleton material, placing into a sample tube, freezing at low temperature, putting into the microwave heating device, carrying out microwave heating on the skeleton material in the sample tube while rotating, removing an end cover and a base, putting into a stainless steel reaction kettle, opening a throttle valve to fully react methane gas and ice particles, and injecting artificial seawater to suspend the skeleton material in hole water so as to prepare the suspended natural gas hydrate. When the microwave heating is adopted, the heating is remarkable, the interior of a sample absorbs less microwave quantity, the heating is not remarkable, the temperature gradient at low-temperature freezing is compensated, and advantages are created for generating the uniform suspended natural gas hydrate by the reaction of the methane gas and the ice particles. The skeleton material is subjected to surface treatment, so that the skeleton material has hydrophobic property so as to avoid from covering a natural gas hydrate layer on the skeleton material.

The invention discloses an electrochemical experiment method for rapidly evaluating the corrosion resistance of a coating. The electrochemical experiment method comprises the following steps of: (1) soaking a steel plate with the coating in artificial seawater for 20-800 hours, and stripping the coating on one surface of the steel plate; (2) loading the treated steel plate into an electrochemical device, connecting a positive electrode and a negative electrode of the electrochemical device to an external electrochemical workstation, and setting impedance test parameters of the electrochemical workstation; (3) carrying out constant potential polarization on the system until an open circuit potential is stable, and starting to measure EIS to obtain an impedance spectrum; and (4) calculating the area under the curve of a Bode diagram, acquiring the area under curve of each soaking stage, acquiring the area change rate, and further rapidly evaluating the corrosion resistance of the coating. The electrochemical experiment method provided by the invention is simple and convenient, real-time detection is performed on site, and the corrosion resistance of the coating is rapidly evaluated.

The present invention relates to artificial seawater and its use to treat a variety of skin conditions, when that sea water has more magnesium in it than in naturally occurring seawater. The treatment of acne and the like can be improved with the product of the present invention.