127 results about "Artificial seawater" patented technology

The invention discloses a pesticide biological activity determination method which comprises the following steps that: (1) a sample (pesticide raw medicine or plant extract) is prepared into 10g/L mother solution by distilled water, the sample which is insoluble in water is first added with a small amount of acetone to be dissolved, and is gradually diluted by artificial seawater to the sample concentration; (2) 10mu L of artemia liquid is shifted to a 24-hole culture plate, and then 1.5ml sample liquid pesticide to be tested is added in to be put into a 25DEG C light incubator to be cultured, and no bait needs to be fed during the test; each treatment is repeated for four times, and simultaneously, the artificial seawater which contains a certain amount of acetone is set for reference; and (3) after 48h of culture, the number of dead artemia nauplii is observed under a anatomical lens, the corrected mortality is calculated, and the insecticidal biological activity of a sample to be tested is evaluated. The method provided by the invention has the advantages of sensitivity to the activity of most pesticide, wide sources, easy largely feeding, low determination cost, simple and convenient method and trace amount of required sample, and is particularly applicable to the screening or the determination of the trace amount of sample.