46 results about "Spermatophore" patented technology

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

The invention discloses a directional mating method for Penaeus vannamei Boone. The method includes the steps that parent prawns are selected and subjected to nutrient enrichment cultivation, a mating scheme is set, male prawns are ripened and independently cultivated, female prawns are ripened and independently cultivated, directional mating parent prawns are selected and subjected to directional mating, and spawning and fertilization are performed. The method is designed according to the life habits and the mating, fertilization and spawning characteristics of the Penaeus vannamei Boone, and possible injuries to the male prawns and the female prawns in the artificial fertilization process can be avoided. In the whole mating process, it is only needed to determine the serial numbers of the parent prawns for mating, operation is convenient and easy, and the genealogical relationship of established families can be accurately established. The genetic relationship of the individuals of the families is determined, the mating scheme is designed artificially, and inbreeding depression of the established families can be effectively avoided. The method has the advantages of being high in success rate and low in parent prawn loss, and overcomes the defects that in the prior art, when Penaeus vannamei Boone families are established in an artificial fertilization mode, spermatophore is prone to falling off, the fertilization rate is low, and half sibs cannot be established easily.

The invention discloses an indoor cultivation method of litopenaeus vannamei parent, and relates to the breeding of seawater shrimps. The method disclosed by the invention selects the healthy shrimp seed without carrying white spot syndrome virus, and is characterized in that the parent cultivation is performed in a cement nursery pond in a seed cultivation room; the water change amount of the cement nursery pond is adjusted according to the body length of the shrimp seeds; the shrimp seeds are fed with compound feed before the body length reaches 10cm; the shrimp seeds are fed with the mixture of compound feed and fresh shredded squid when the body length reaches 10cm; when the body length reaches 12cm, the shrimp seeds are fed with fresh shredded squid; after the shrimp seeds are longer than 14cm, the spermatophore of the male shrimp occurs, the shrimp seeds are fed with fresh fait with gonad of clamworm and oyster meat; before the shrimp seeds reach the parent specification, the cultivation salinity is kept at 22; when the shrimp seeds reach the parent specification and the spermatophore of a few male shrimps is mature, the salinity is adjusted to 30; and in the next day after a great amount of water in the nursery pond is changed, 10ml / m<3> of composite bacterial liquid consisting of bacillus subtilis, lactic acid bacteria and saccharomycetes is added once per day.

The invention discloses a biological engineering method of squids, which comprises the steps of selecting a masculine adult squid, pinching off spermatophore thereof, and immersing the spermatophore in low-temperature seawater containing adapting reagent; putting a feminine squid in a container filled with low-temperature seawater for temporary culture for 5 to 30 minutes; charging CO2 into the low-temperature seawater; taking the feminine squid out, and implanting the spermatophore into a mantle of the feminine squid; putting the feminine squid after insemination back to the container filled with low-temperature water, charging CO2 into the seawater and boosting pressure; reducing pressure to normal, and then increasing the temperature of water gradually. The spermatophore is immersed in the low-temperature seawater containing the adapting reagent, and after insemination, failures caused by pinch-off of the spermatophore by a tentacle of a female parent due to inadaptation to the spermatophore can be effectively reduced. The biological engineering method of squids is applicable to biological engineering operation of homologous or heterogenetic squids, has the advantages of high hatching rate, high survival rate, little disease, easy culture, strong disease resistance, rapid growth and the like, and the biological engineering method has easy implementation and high success rate.

The invention discloses a method for storing spermatophore of litopenaeus vannamei at low temperature. The method comprises the following steps of: acquiring the spermatophore, fixing the spermatophore, storing the spermatophore and dyeing spermatozoa. In the method, a culture layer for the spermatophore of the litopenaeus vannamei is prepared from 0.3 to 0.4 part of 1.8 to 3.0 percent self-prepared type I liquid rat tail collagen serving as a medium for contracting the culture layer, 0.3 to 0.4 part of culture solution of 2*1,640, 0.1 to 0.2 part of fetal calf serum in an American GIBCO company and 0.1 part of Matrigel (a soluble basilar membrane matrix) in an American Sigma company, wherein the culture layer is viscous at normal temperature, and can be solidified at the temperature of 40 DEG C and can wrap the spermatophore. The spermatophore is packaged in separate an epoxy resin (EP) tube at the normal temperature, the spermatophore is wrapped by the prepared culture layer, and the EP tube wrapped with the spermatophore is arranged in a refrigerator to be stored at the low temperature of 4 DEG C. The method has the characteristics of simplicity of operation, complete storage of a spermatophore structure, high survival rate of the spermatozoa, long retention time, stable effect of low-temperature storage and the like and is easy to popularize and apply. The retention time of the spermatophore is at least 3 months, and the survival rate of the spermatozoa is over 80 percent.

The invention discloses a method for extracting macrobrachium spermatophore through a pulse electric shock method and a macrobrachium intraspecific crossbreeding method. The methods comprise the steps that two pulse electrode tips are put on the portions close to the rear sides of two fifth paraeiopod bases, a connecting lead between the two electrode tips is parallel to the junction loop line of the cephalothorax and the abdomen of male macrobrachium, the electrode tips are basically perpendicular to the abdomen surface of the male macrobrachium, a pulse power switch is gently clicked once or twice, and the male macrobrachium is simulated through pulse electricity; a person pays close attention to spermatophore discharging holes formed in the inner sides of the fifth paraeiopod bases, takes down the spermatophore as soon as finding the spermatophore with ophthalmological tweezers and rapidly stick the spermatophore to the portions between the fourth paraeiopod bases and the fifth paraeiopod bases of congener female macrobrachium which just finishes reproductive uncoating, and the female macrobrachium conducts oviposition and incubation by itself. According to the method for extracting the macrobrachium spermatophore through the pulse electric shock method and the macrobrachium intraspecific crossbreeding method, the spermatophore can be extracted from the male macrobrachium rapidly, effectively and safely, the pulse electricity is adopted to extract the spermatophore, the male macrobrachium is not damaged, and compared with stimulation through an ordinary alternating current, the extracting success rate, the transplanting success rate and the fertilization rate are all obviously increased.

The invention discloses a non-directional mating litopenaeus vannamei full-sib family batch preparation method. According to defects of an existing litopenaeus vannamei family preparation technology, the method in which non-directional mating is used, litopenaeus vannamei beard tissue is sheared to extract DNA, and remaining sperms in spermatophores of female litopenaeus vannamei obtained after oviposition serve as a male parent source basis is designed, a male parent individual and a female parent individual are determined by means of an existing mature microsatellite parentage identification technology, and a full-sib family is prepared. The method which combines high non-directional random mating efficiency and high microsatellite parentage identification accuracy is used, the defects of the existing litopenaeus vannamei family preparation technology are made up for, and the method is beneficial to litopenaeus vannamei genetic breeding.

The invention discloses a cultivation method of Homarus seeds. The method is characterized in that when the gonad index of male Homarus parents reaches 5-20%, zinc sulfate, monocalcium phosphate and vitamin C are added to female Homarus parents and the temperature is risen 1-3 DEG C each day to accelerate the gonad maturity of the female Homarus parents so as to allow the female Homarus parents to molt, the molted female Homarus parents and the male Homarus parents are cultivated in a mixed manner, the spermatophores of the male Homarus parents can be easily inserted into the molted female Homarus parents, and mating rate is increased greatly; the mated female Homarus parents are cultivated independently, cultivation conditions such as spawning and hatching and larva cultivation are controlled until the Homarus seeds are obtained, and fully-artificial Homarus seed cultivation is achieved successfully.

The invention provides a method for preventing and treating black spermatophore disease of Litopenaeus vannamei, falling into the field of aquaculture disease prevention and treatment. The method is mainly realized through technical measures including aquaculture water treatment, aquaculture environment control, feeding of high-quality baits, drug application and artificial black spermatophore removal by extrusion. The method has convenient application, curative rate up to above 90%, low death rate of male prawn, and no need of cooperation with ripened female prawn. Female prawn ripening and spawning can be performed after re-culture and ripening of diseased male prawn. The method can realize reasonable production arrangement and save production cost.

The invention discloses a litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method. By performing semi-directional mating between one female shrimp and many male shrimps, the mating success rate is ensured, and then by examining whether the spermatophore of the male shrimps is empty or not one to one, a male parent is determined. According to the litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method, a litopenaeus vannamei full sibs family can be efficiently constructed, and a large quantityof the full sibs family filial generations are obtained; by the adoption of the method, the parent of the full sibs family can be conveniently and fast determined so that heredity and trait characteristics of the full sibs family parent can be completely obtained. The method provides important technological support for litopenaeus vannamei molecular marker assistant breeding.

The invention relates to a method for obtaining Neoseiulus californicus spermatophores and observing the Neoseiulus californicus spermatophores under a transmission electron microscope. The method ischaracterized in that spermatophores are obtained from the male gnathosoma of mating Neoseiulus californicus, and are observed under the transmission electron microscope, and the method includes the steps of obtaining of male and female Neoseiulus californicuss, setting of the male and female Neoseiulus californicuss, separation of the male and female Neoseiulus californicuss, obtaining of the spermatophores, and preparation and observation of a spermatophore transmission electron microscopy sample. The Neoseiulus californicus male spermatophores can be obtained through a very simple and ingenious technology, and the ultrastructural observation of the internal structure and substances of the outer spermatophores of the Neoseiulus californicus is carried out by using the transmission electron microscope, so the form and the quantity of sperms in the outer spermatophore and the change (such as heterogenization) or not of the sperms before reaching female Neoseiulus californicus are clearly known, thereby the determination of whether the male Neoseiulus californicus plays a decisive role in the gender determination process or not is benefited.

The invention relates to a method for obtaining and preserving spermatophores of Amblyseius tsugawai. The method is characterized in that spermatophores are obtained and picked from gnathosoma of maleAmblyseius tsugawai in the mating process, the method includes the steps of acquisition of females and males in the mating process, setting of the females and the males, separation of the females andthe males and acquisition and preservation of the spermatophores, and all operations are carried out under the microscope except setting of the females and the males in the mating process. The spermatophores of the male Amblyseius tsugawai can be obtained by means of the extremely easy and smart method, and acquisition of the spermatophores can provide a material source for research of the spermsof Amblyseius tsugawai; the method can also provide reference for research of the spermatophores of the other predatory mites.

The invention provides application of a TGL gene to prevention and control of sitotroga cerealella, and relates to the technical field of insect prevention and control. When the expression of the TGLgene is suppressed, the decomposition rate of triacylglycerol is reduced, so that sufficient mating contents are not synthesized in male insect bodies, and therefore, no more contents can be transferred to female insects in the mating process, normal spermatophores cannot be formed inside copulatory bursa, and eventually mature eggs cannot be successfully fertilized.

The invention relates to a method for acquiring predatory mite spermatophore. The method is characterized by a method for artificially acquiring and picking spermatophore from phytoseiidae predatory mite capitulum in the mating process. The method comprises the following steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; and acquiring and storing spermatophore. All the operations, except for the shaping of the male and female mites in the mating process, are performed under the microscope. The invention utilizes a simple and smart method to acquire the predatory mite spermatophore; the acquisition for the spermatophore is capable of supplying material source for the research on the male mite sperm; the invention also can supply reference for the acquisition for the other colony predatory mite spermatophore.

The invention provides a method for replanting waste spermatophore of marsupenaeus japonicus, relating to a method for artificial propagation of marine shrimps. The method for replanting waste spermatophore of marsupenaeus japonicus, which is provided by the invention, comprises the following steps: stripping the original complete spermatophore from the seminal receptacle of the female broodstockof the marsupenaeus japonicus which just dies and molts, and cleaning the spermatophore for later use; and taking the female broodstock of the marsupenaeus japonicus the carapace of which is not hardened, sticking a bonding agent at the bottom of the spermatophore, filling the spermatophore into the seminal receptacle of the female broodstock, and then, sterilizing the female broodstock for culturing. The method provided by the invention has simple and understandable principles and is simple and convenient in operation, and the maturing production process of the female broodstock can be carried out at any time according to the needs; and the used bonding agent has wide material sources and is economical and practical. The invention fills the blank of artificial mating of the molted femalebroodstock of the marsupenaeus japonicus in natural sea areas, and has the advantages of very low risk factor in application and obvious economical benefit.

The invention relates to a method for observing predatory mite spermatophore under a transmission electron microscope. The method is characterized by a method for acquiring spermatophore from male phytoseiidae predatory mite capitulum in the mating process and then observing the spermatophore under the transmission electron microscope. The method comprises the following five steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; acquiring spermatophore; preparing and observing a spermatophore transmission electron microscope sample. The invention utilizes a simple and smart method to acquire the male phytoseiidae predatory mite spermatophore; the transmission electron microscope is utilized to ultra-micro observe the inner structure and matter of the spermatophore of the predatory mite; through observation, we can further know the form and quantity of the sperms in the spermatophore and if the sperms have changed (such as, heterogenization) before entering into the female mite body, so that people can confirm if the male phytoseiidae predatory mite plays a decisive role in a sex determination process.

The invention relates to a method for acquiring phyoseiulus persimilis spermatophore. The method is characterized by being the method for manually acquiring and picking spermatophore from male phyoseiulus persimilis capitulum in the mating process. The method comprises the following four steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; and acquiring and storing spermatophore. All the operations, except for the shaping of the male and female mites in the mating process, are performed under the microscope. The invention utilizes a simple and smart method to acquire the phyoseiulus persimilis spermatophore; the acquisition for the spermatophore is capable of supplying material source for the research on the phyoseiulus persimilis sperm; the invention also can supply reference for the acquisition for the other colony predatory mite spermatophore.

The invention relates to a method for observing a spermatophore of a predatory mite under a scanning electron microscope. The method is characterized by steps of acquiring a predatory mite male with an easy-to-see spermatophore at first and then preparing and observing the spermatophore under the scanning electron microscope, and comprises five parts of acquisition of predatory mite male and female in mating, sizing of predatory mite male and female, separation of predatory mite male and female, storage of predatory mite male, and spermatophore scanning electron microscope specimen preparationand observation. According to the invention, the easy and ingenious method is utilized to acquire the spermatophore of the predatory mite male, and realize the purpose of observing the tiny externalstructure of the spermatophore under the scanning electron microscope; the acquisition of the spermatophore provides a raw material for observing the structure of the spermatophore under the scanningelectron microscope, and a result of the scanning electron microscope facilitates the foundation of research on other respects of the spermatophore and gives enlightenment.

The invention relates to a litopenaeus vannamei male parent semi-directional mating full-sib family construction and parent determination method. The method sequentially comprises the following steps of female parent management, breeding and screening of candidate mating male parents, male parent semi-directional mating, parent determination, fertilized egg treatment, larva breeding and shrimp seed cultivation. According to the method, in combination with the breeding habits and characteristics of litopenaeus vannamei, one male shrimp with milky spermatophore, full sperm mass filling and high chasing ability is screened out from the candidate mating male parents, and the male shrimp and five female shrimps with full ovaries are subjected to semi-directional mating, so that the mating success rate is increased. According to the method, the full-sib family of the litopenaeus vannamei can be quickly and efficiently constructed, a large number of full-sib family filial generations can be obtained, and parents of the full-sib family can be simply and quickly determined, so that genetic and character characteristics of the parents of the full-sib family can be completely and accurately obtained; and therefore, an important technical support is provided for molecular marker-assisted breeding of the litopenaeus vannamei.

The invention relates to a phytoseiulus persimilis sperm acquisition and slide specimen preparation method. The method is characterized in that the method for preparing a slide specimen by acquiring spermatophore from gnathosoma of male phytoseiulus persimilis in mating includes acquisition of male and female phytoseiulus persimilis in mating, fixing the male and female phytoseiulus persimilis, separation of the male and female phytoseiulus persimilis, spermatophore acquisition, sperm acquisition and semen slide specimen preparation. Spermatophore acquisition provides raw materials for phytoseiulus persimilis sperm slide specimen preparation and research, and semen slide specimen preparation provides references for knowing morphology of phytoseiulus persimilis sperms and revealing phytoseiulus persimilis reproduction principles.

The invention relates to a method for acquiring and observing neoseiulus californicus spermatophores under a scanning electron microscope. The method is characterized in that male neoseiulus californicus easily seen spermatophores are firstly acquired, and the spermatophores are prepared and observed under the scanning electron microscope. The method includes the steps: acquisition of male and female neoseiulus californicus in mating; shaping of the male and female neoseiulus californicus; separation of the male and female neoseiulus californicus; storage of the male neoseiulus californicus; preparation and observation of spermatophore scanning electron microscope specimens. The male neoseiulus californicus spermatophores can be acquired by a quite simple, easy and ingenious method, fine external structures of the spermatophores can be observed under the scanning electron microscope, acquisition of the spermatophores can provide raw materials for observation of the spermatophore structure under the scanning electron microscope, and results of the scanning electron microscope lay a foundation and provides an enlightenment for research of other directions of the spermatophores.

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

The invention relates to a method for obtaining Amblyseius tsugawai spermatophores and observing the Amblyseius tsugawai spermatophores under a transmission electron microscope. The method is characterized in that spermatophores are obtained from the male gnathosoma of mating Amblyseius tsugawai, and are observed under the transmission electron microscope, and the method includes the steps of obtaining of male and female Amblyseius tsugawais, setting of the male and female Amblyseius tsugawais, separation of the male and female Amblyseius tsugawais, obtaining of the spermatophores, and preparation and observation of a spermatophore transmission electron microscopy sample. The Amblyseius tsugawai male spermatophores can be obtained through a very simple and ingenious technology, and the ultrastructural observation of the internal structure and substances of the outer spermatophores of the Amblyseius tsugawai is carried out by using the transmission electron microscope, so the form and the quantity of sperms in the outer spermatophore and the change (such as heterogenization) or not of the sperms before reaching female Amblyseius tsugawai are clearly known, thereby the determinationof whether the male Amblyseius tsugawai plays a decisive role in the gender determination process or not is benefited.

The invention relates to a method for obtaining sperms from predatory male mites and making slide specimens. The method for obtaining the sperms from the predatory male mites and making the slide specimens is characterized by being the method for making the sperm slide specimens after obtaining spermatophore from male mite gnathosoma of the predatory mites in phytoseiidae under mating, comprisingfive parts of acquiring male and female mites under mating, stereotyping the male and female mites, separating the male and female mites, acquiring the spermatophore, acquiring the sperms, and preparing the slide specimens. By adopting the method for obtaining the sperms from the predatory male mites and making the slide specimens disclosed by the invention, the spermatophore of the predatory malemites can be obtained by using a very simple and ingenious method, and the sperm slide specimens can be made, the spermatophore is obtained, thereby being capable of providing raw materials for making research on the sperm slide specimens of the predatory mites, and the sperm slide specimens can be made, thereby being capable of providing a reference for understanding the morphology of the predatory mite sperms and revealing reproduction principles of the predatory mites.

The invention discloses a transurethral filtering screen expansion device for ejaculatory duct obstruction, wherein the transurethral filtering screen expansion device comprises a spermatophore exploring tube capable of being inserted into an ejaculatory duct, wherein an isolation plate extending in the axial direction is arranged in the spermatophore exploring tube; the isolation plate separates an inner cavity of the spermatophore exploring tube into an observation cavity and an expansion rod accommodating cavity; the front end of the observation cavity is provided with a high-light-transmission objective lens; the high-light-transmission objective lens is externally connected with a central processing unit through a visible optical fiber; a display is arranged on the central processing unit; images shot by the high-light-transmission objective lens can be displayed on the display; the transurethral filtering screen expansion device also comprises an expansion rod; the front end of the expansion rod is sleeved by an elastic filtering screen; the expansion rod can be inserted into the expansion rod accommodating cavity and can axially slide in the expansion rod accommodating cavity; when the elastic filtering screen is arranged in the expansion rod accommodating cavity, the state is a compression state; when the elastic filtering screen extends out from the expansion rod accommodating cavity, the elastic filtering screen expands; the outer diameter is enlarged.

The invention discloses a litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method. By performing semi-directional mating between one female shrimp and many male shrimps, the mating success rate is ensured, and then by examining whether the spermatophore of the male shrimps is empty or not one to one, a male parent is determined. According to the litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method, a litopenaeus vannamei full sibs family can be efficiently constructed, and a large quantityof the full sibs family filial generations are obtained; by the adoption of the method, the parent of the full sibs family can be conveniently and fast determined so that heredity and trait characteristics of the full sibs family parent can be completely obtained. The method provides important technological support for litopenaeus vannamei molecular marker assistant breeding.

The invention relates to a manufacturing method of a male Amblyseius tsugawai semen slide specimen. The manufacturing method is characterized in that the manufacturing method is a method for manufacturing a semen slide specimen after acquiring a spermatophore from the jaw body of male Amblyseius tsugawai which is mating, and comprises the following five parts such as acquisition of mating male andfemale Amblyseius tsugawai, shaping of male and female Amblyseius tsugawai, separation of male and female Amblyseius tsugawai, acquisition of the spermatophore, acquisition of semens and slide specimen preparation. The spermatophore of the male Amblyseius tsugawai can be acquired and the semen slide specimen can be manufactured by using an extremely simple and smart method, the acquisition of thespermatophore can provide raw materials for the manufacturing and research of the male Amblyseius tsugawai semen slide specimen, and the manufacturing of the semen slide specimen can provide reference for understanding the shape of Amblyseius tsugawai semens and disclosing the reproduction principle of Amblyseius tsugawai.