A method for tracing the parents of Litopenaeus vannamei in random mating

A technology of Litopenaeus vannamei and Penaeus prawn, which is applied in the field of genetic breeding, can solve problems such as non-mating of male shrimp, skewed sperm pods, hindering molecular breeding of shrimp and selection of excellent parents, and achieves accelerated preparation, high efficiency, and overcoming spermatozoa. The effect of incorrect pod position

Active Publication Date: 2019-06-11
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

However, Litopenaeus vannamei has the characteristic of embracing and chasing tails, and then separates after mating. The sperm pods of the male shrimp must penetrate into the seminal vesicle of the female shrimp to complete the mating. Often there will be multiple male shrimp and female shrimp. The phenomenon of penetrating into the seminal vesicles, artificial directional mating and male shrimp not mating, or single tail mating but the sperm pods are distorted and other unfavorable factors
Unfavorable factors in the breeding process of Litopenaeus vannamei greatly limit the preparation process of full-sib families, hindering the molecular breeding of shrimp and the selection of excellent parents

Method used

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Examples

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Effect test

Embodiment 1

[0014] 1. Male shrimp DNA library preparation: Litopenaeus vannamei after sexual maturity during the breeding period were reared separately, male shrimp were reared in male shrimp ponds, female shrimp were reared in female shrimp ponds, and all 80 male shrimp in the male shrimp ponds were finally Cut off a pair of swimming legs, store them in 95% ethanol by volume at -20°C, then extract the DNA of the swimming legs and number them separately (male 1, 2, 3...80) to form a male shrimp DNA library;

[0015] 2. Random mating of parent shrimps: at 7-11 am, select 15 female shrimps whose gonads are full and extend to the tail and put them into the male shrimp pond, and the male shrimps and female shrimps mate freely;

[0016] 3. Spawning of female shrimp: From 18 to 21 o'clock on the same day, fish out 15 female shrimps, check whether the seminal vesicles have been injected into the sperm pods, and obtain 10 female shrimps that have correctly injected sperm pods, and each of them has...

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Abstract

The invention discloses a panmixis parent tracing method for litopenaeus vannamei. The purposes of panmixis of litopenaeus vannamei and post parent tracing and identification are achieved in combination with a multiplication characteristic that spermatophore is still remained in the body of female litopenaeus vannamei after the litopenaeus vannamei spawns, and a litopenaeus vannamei microsatellite paternity test method which is mature currently. According to the method provided by the invention, the advantage that the female litopenaeus vannamei is high in successful mating efficiency during a production process is used, and the deficiency that the male does not mate with the female in directional mating and the position of the spermatophore is incorrect after mating is overcome; application of the method provided by the invention quickens the preparation of the full-sib families of the litopenaeus vannamei, and is beneficial to prompting the operation of litopenaeus vannamei molecular breeding.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to a method for tracing the random mating parents of Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei (Litopenaeus vannamei, also known as Penaeus vannamei) aquaculture occupies a relatively large proportion in China's fishery economy and is one of the pillar industries of aquaculture in my country. However, the genetic breeding of Litopenaeus vannamei in my country is very backward, and the high-quality broodstock has long been dependent on imports. This situation has brought great hidden dangers to the health, stability and sustainable development of my country's shrimp industry. Therefore, strengthening the basic and applied research in the field of Litopenaeus vannamei genetics and breeding will help to improve the unfavorable situation of my country's Litopenaeus vannamei industry. [0003] It is an indispensable link in molecular breeding to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59
CPCY02A40/81
Inventor 黄文陈廷任春华王艳红江晓胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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