A method for tracing the parents of Litopenaeus vannamei in random mating
A technology of Litopenaeus vannamei and Penaeus prawn, which is applied in the field of genetic breeding, can solve problems such as non-mating of male shrimp, skewed sperm pods, hindering molecular breeding of shrimp and selection of excellent parents, and achieves accelerated preparation, high efficiency, and overcoming spermatozoa. The effect of incorrect pod position
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[0014] 1. Male shrimp DNA library preparation: Litopenaeus vannamei after sexual maturity during the breeding period were reared separately, male shrimp were reared in male shrimp ponds, female shrimp were reared in female shrimp ponds, and all 80 male shrimp in the male shrimp ponds were finally Cut off a pair of swimming legs, store them in 95% ethanol by volume at -20°C, then extract the DNA of the swimming legs and number them separately (male 1, 2, 3...80) to form a male shrimp DNA library;
[0015] 2. Random mating of parent shrimps: at 7-11 am, select 15 female shrimps whose gonads are full and extend to the tail and put them into the male shrimp pond, and the male shrimps and female shrimps mate freely;
[0016] 3. Spawning of female shrimp: From 18 to 21 o'clock on the same day, fish out 15 female shrimps, check whether the seminal vesicles have been injected into the sperm pods, and obtain 10 female shrimps that have correctly injected sperm pods, and each of them has...
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