A kind of half-sib family construction method of Penaeus vannamei

Abstract

The invention discloses a penaeus vannamei boone half-sib family construction method. The method comprises the steps: selecting mature parent shrimps, marking an individual parent shrimp, setting a mating scheme, acquiring spermatophore, activating sperms, laying eggs by female shrimps, collecting eggs, performing artificial insemination, hatching germ cells, collecting nauplius, and the like. Compared with the traditional artificial spermatophore transplanting method, the method disclosed by the invention has the advantages that no harm on the female shrimp is produced, and a plurality of half-sib families can be simultaneously established; the sperms are artificially released into an egg laying water body, so that the weakness of no mating or separation of the mated female shrimp spermatophore caused by the natural mating can be avoided; on the premise of a set inbreeding coefficient, the mating scheme is artificially designed, so that the inbreeding depression of the constructed family can be effectively avoided, and the method is a high-efficient penaeus vannamei boone half-sib family construction method.

Description

technical field [0001] The invention relates to a line selection method of Penaeus vannamei, in particular to a method for constructing half-sib lines of Penaeus vannamei, belonging to the technical field of aquaculture. Background technique [0002] The Penaeus vannamei industry is a pillar industry of my country's fishery economy, and there is a huge demand for Penaeus vannamei seedlings in my country every year. However, the high-quality broodstock of Penaeus vannamei in my country still mainly depends on imports, which seriously restricts the development of my country's Penaeus vannamei industry. Therefore, strengthening the breeding of fine varieties of Penaeus vannamei is the key to realizing the sustainable development of my country's vannamei industry. [0003] In recent years, the Hawaii Oceanographic Research Institute in the United States has bred fast-growing strains and taura-resistant Penaeus vannamei strains through family selection combined with comprehensiv...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for constructing half-sib families of Penaeus vannamei, which has a high fertilization rate, does not need artificial sperm pod transplantation, does not cause harm to female shrimp, and can simultaneously establish multiple In the half-sib family, the sperm are artificially released into the spawning water body, which can avoid the defects of non-mating caused by natural mating or the loss of sperm pods of female shrimp after mating, and the mating plan can be artificially designed under the premise of the set inbreeding coefficient , which can effectively avoid inbreeding depression in the established family, and is an efficient method for constructing the family of Penaeus vannamei