131 results about "Germ cell" patented technology

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The present invention relates to lipid nanoparticle (LNP) compositions, as well as diagnostic or therapeutic polynucleotides, such as TERT mRNA, that can be delivered in a formulation together with the LNP compositions to various tissue and cell types in the whole body of a mammal, such as, for example, TNP mRNA. Comprising stem cells, progenitor cells, germ cells, differentiated cells or terminally differentiated cells, cancer cells, endothelial cells, epithelial cells, splenic cells, hepatocells, kidney cells and / or osteoblasts, for example, for use in the diagnosis, prevention and / or treatment of a condition or disease.

The present disclosure relates to methods, products, and compositions useful for deriving bovine induced blastoids. Methods are described that allow for juvenile stem cell aggregation and induced blastocyst formation. The invention further provides an aggregation culture medium and an induced blastocyst-like culture medium which are suitable for promoting aggregation of the young stem cells and preparing the induced blastocyst-like. The bovine induced blastoids can be used in selection and breeding procedures, including the replication of pre-implant embryos with desired characteristics and in vitro breeding including a cross-generation breeding cycle from parent germ cells and / or embryos to progeny embryos.

A system for determining at least one biomechanical property of a reproductive cellular structure includes a radiation source for generating illuminating radiation, at least one optic for directing the illuminating radiation onto at least a portion of the reproductive cellular structure, a detector for detecting at least a portion of radiation scattered from the reproductive cellular structure, a spectrometer for generating a frequency spectrum of the detected scattered radiation, and an analyzer. The analyzer is configured to identify at least one Brillouin frequency shift in the frequency spectrum for the at least a portion of the reproductive cellular structure, and determine viability of the reproductive cellular structure based on the at least one Brillouin frequency shift.

The present invention addresses the problem of providing a technique capable of haploid induction by enhancing the expression and / or function of a regulatory gene. The problem is solved by the use of a modified plant comprising an introduced or modified H2A.W gene, wherein said introduction or modification enhances the expression and / or function of the H2A.W gene in a germ cell.

Bispecific antibody constructs comprising a first binding domain, which binds to human alkaline phosphatase, placental type (ALPP) and / or alkaline phosphatase, germ cell type (ALPPL2), on the surface of a tumor cell and a second binding domain, which binds to human CD3 on the surface of a T cell.

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

A transdermal composition can include a cannabis particulate blend including THCA, CBD, and male and female reproductive cells, wherein the cannabis particulate blend has a particle size distribution within a range of about 120 µm to about 400 µm, and wherein the THCA is present in the transdermal composition at from about 0.01 wt% to about 0.5 wt% THCA and the CBD is present in the transdermal composition at from about 1 wt% to about 10 wt% CBD. The transdermal composition can further include from about 1 wt% to about 10 wt% lion's mane mushroom, and from about 50 wt% to about 85 wt% coconut oil and / or butter and about 5 wt% to about 20 wt% beeswax based on a total weight of the transdermal composition. The THCA in this example is prepared to include particles from both the male plant and the female plant.

The application belongs to the technical field of biology and particularly relates to a functional recombinant Cas9 protein targeted to an ovocyte of Procambarus clarkii and application thereof. The protein is NLs-VgSP-Cas9-NLs. The application directly delivers the recombinant Cas9 protein, thereby reducing the risk of integration of exogenous genes. By specifically targeting the ovocyte, off-target effects on somatic cells can be avoided, the efficiency of editing of germ cells is improved, and early development or genetic manipulation can be conveniently studied. The ovocyte of Procambarus clarkii is large, the recombinant Cas9 protein is efficiently delivered by receptor-mediated endocytosis on the surface of the ovocyte, and the problem that microinjection of zygotes cannot be implemented is solved.

The invention belongs to the technical field of biology, and particularly relates to cherax quadricarinatus oocyte targeted functional recombinant Cas9 protein and application thereof. Specifically, the protein is NLs-VgSP-Cas9-NLs (Nuclear Leukemia Sequence-Vector Through direct delivery of the recombinant Cas9 protein, the risk of exogenous gene integration is reduced. Through specific targeting of oocytes, off-target effect on somatic cells can be avoided, germ cell editing efficiency is improved, and research of early development or genetic manipulation is facilitated. The cherax quadricarinatus oocytes are large, the recombinant Cas9 protein is efficiently delivered through endocytosis mediated by oocyte surface receptors, and the problem that microinjection of fertilized eggs cannot be implemented is solved.

The invention provides a cell culture box for assisted reproduction, and relates to the technical field of cell culture. The cell culture box for assisted reproduction comprises a box body, the top of the box body is slidably connected with a protective shell, the interior of the protective shell is fixedly connected with a microscope used for observing germ cell production, the bottom of the microscope is provided with a lens, the rear wall of the box body is fixedly connected with an electric push rod, and the electric push rod is fixedly connected with the protective shell. The movable end of the electric push rod is fixedly connected with a connecting plate, the other end of the connecting plate is fixedly connected with the protective shell, the inner bottom of the box body is rotationally connected with a rotating column through a bearing, and the top end of the rotating column is fixedly connected with a rotating disc. By arranging an electric push rod, a connecting plate, a protective shell, a microscope, a lens, a rotating column, a rotating disc and a rotating assembly, a technician can conveniently observe the growth condition of cells without taking out the germ cells, and the number of times of opening the door body is reduced, so that the influence on germ cell culture is reduced.

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

The present disclosure provides bovine animals and methods to create recipient bovine animals for spermatogenic stem cell transplantation / complementation through modulation of the NANOS2 gene. In one embodiment genome editing was used to create bovine animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS2 gene activity so that resulting male bovines lack functional germ cells yet retain functional testicular somatic cells, and bovine females are fertile. These bovine males can then be transplanted or complemented with donor cells capable of giving rise to cells of the spermatogenic lineage, or spermatogenic stem cells and used for breeding.

A method of measuring at least one biomechanical property of a reproductive cellular structure is provided. The method includes illuminating the reproductive cellular structure with radiation; detecting at least a portion of radiation scattered from the illuminated reproductive cellular structure; analyzing a frequency spectrum of the detected scattered radiation to identify at least one Brillouin frequency shift in the frequency spectrum; and determining the at least one biomechanical property based on the Brillouin frequency shift. The method further includes determining a viability index of the reproductive cellular structure based on the at least one biomechanical property.

In some aspects, the present disclosure provides germ cell nuclear factor ligands of the formula:wherein the variables are defined herein, as well as pharmaceutical compositions and methods of use thereof. Also provided are cell cultures and cell culture media comprising the GNCF ligands of the present disclosure as well as methods of use thereof.

Provided is a method for obtaining the germ cell lineage of an oviparous vertebrate more efficiently than conventional techniques. A host oviparous vertebrate is prepared at a developmental stage after the development of black pigmentary cells in the retina and before the formation of multiple layers of germ cells in the genitals, and isolated undifferentiated germ cells from a donor oviparous vertebrate are transplanted into the host oviparous vertebrate.

The invention belongs to the technical field of bamboo reed breeding, and discloses a mutation breeding method for obtaining a bamboo reed cold-resistant mutant based on EMS (Enhanced Mass Spectrometry), which comprises the following steps: step 1, inducing embryogenic callus; step 2, carrying out EMS mutagenesis treatment; step 3, performing EMS stress screening; 4, differentiation and rooting culture of adventitious buds; 5, screening the tissue culture seedlings at low temperature; the mutation obtained through mutagenesis treatment is a permanent change of a genetic structure of an organism and may appear in germ cells and somatic cells of a plant. Physical or chemical mutagenic agents are used to increase the frequency of mutations. In chemical mutagens, EMS is considered to be the most effective mutagen, the EMS can generate a large number of non-lethal point mutations in the whole genome, the mutation frequency is high, the chromosome abnormality frequency is low, the EMS is used for treating embryogenic calluses of the bamboo reeds, Hyp is used for screening, the low-temperature-resistant bamboo reed mutants are obtained, the planting area of the bamboo reeds can be enlarged, and the survival rate of the bamboo reeds is increased. And waste land in a low-temperature region is fully utilized.

The present disclosure relates to transgenic ornamental Angelfish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic Angelfish and methods of providing them to the ornamental fish industry for the purpose of marketing.

To provide a cryopreservation solution or a cryopreservation method for porcine sperm and porcine embryos (blastocysts), which demonstrates practical cryopreservation effects applicable to production sites.SOLUTION: Cryopreservation of porcine sperm involves using a cryopreservation solution that includes DMGA-PLL (dimethylglytarate-modified polylysine) 0.2-0.3 w / w%, glycerol 2-4 w / w%, trehalose 5-15 w / w%, and yolk 15-25 w / w%. Cryopreservation of porcine embryos (blastocysts) involves using a cryopreservation solution that includes DMGA-PLL (dimethylglytarate-modified polylysine) or CPLL (succinate-modified polylysine) 8-12 w / w%, ethylene glycol 30-35 w / w%, polyethylene glycol 1-3 w / w%, trehalose 15-25 w / w%, and bovine fetal serum 15-25 w / w%.SELECTED DRAWING: Figure 2

The invention discloses an out-of-season artificial propagation technical method for crucian carp. The technical method specifically comprises the key steps of water temperature and illumination control of an intelligent culture system, parent fish screening and concentrated intensified culture, parent fish ripening acceleration, artificial spawning induction, artificial insemination, incubation and the like. Aiming at seasonal limitation of natural breeding of the crucian carp, by scientifically regulating and controlling an illumination period and a water temperature environment and accurately simulating ecological conditions suitable for breeding of the crucian carp, it is ensured that parent fishes of the crucian carp can synchronously reach physiological states of periodic normal ovulation and spermiation in an unnatural spawning season from October to January of the next year; the activity and quality of germ cells can be obviously improved; meanwhile, an innovative breeding mode and an exclusive feeding scheme are combined, the industrial pain points that eggs of female crucian carp of ice cream are prone to aging and parent fishes only spawn once a year in a natural state are effectively overcome, and anti-season stable breeding is achieved.

The present disclosure relates to transgenic ornamental Angelfish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic Angelfish and methods of providing them to the ornamental fish industry for the purpose of marketing.

The invention discloses a method for identifying sheep testicular germ cells by an immunofluorescence technique based on DDX39A gene, and particularly relates to the technical field of animal breeding, which comprises the following steps: S1, embedding treatment; s2, antigen permeation; s3, antigen repair; s4, closing serum; s5, primary antibody incubation: DDX39A is diluted, and a primary antibody solution is prepared; s6, secondary antibody incubation: selecting a green fluorescence labeled antibody for dilution, and preparing a secondary antibody solution; s7, washing; s8, performing nucleus dyeing; s9, mounting and observing; and S10, analyzing the image. The DDX39A is adopted to replace existing VASA to mark germ cells in the testis, compared with the VASA, the DDX39A gene can participate in multiple processes such as RNA splicing, nuclear output, transcriptional regulation and DNA repair, and the DDX39A gene has the advantages of being diversified in function, suitable for multi-aspect research, high in flexibility and wide in sample source and has wide application prospects clinically.

To provide a method for producing a sterile lepidopteran insect by a sterilization method which can simply, stably and efficiently sterilize a lepidopteran insect and causes abnormality only in germ line cells, and a sterilizing composition capable of executing the method.SOLUTION: The expression of nanosO and nanosP genes in a host lepidopteran insect is suppressed.SELECTED DRAWING: None

The present invention provides an endogenous germ cell-free fish, crustacean or mollusk having a chimeric gonad wherein the chimeric gonad comprises at least one transplanted germ cell, the transplanted germ cells have mutations that are present in the germ cell line and disrupt the development and / or function of somatic gonad cells that can be used as parent fish. The present invention also provides a method of preparing a parent fish, crustacean or mollusk for the production of sterilized fish, crustacean or mollusk that is sterilized or sex-determined, and the parent fish themselves.

The invention discloses a rapid breeding method for generating larimichthys crocea sperms based on spermatogonial stem cell transplantation. The rapid breeding method comprises the following steps: step S1, performing whole cryopreservation on larimichthys crocea testis; s2, separating the spermatogonial stem cells of the pseudosciaena crocea; s3, dyeing the spermatogonial stem cells of the pseudosciaena crocea; step S4, treatment of nibea albiflora receptors; and S5, transplanting the dyed donor larimichthys crocea spermatogonial stem cells into the nibea albiflora receptor testis. According to the method, when the nibea albiflora acceptor is treated, the traditional busulfan concentration of 40 mg / kg, twice injection and 35 DEG C high-temperature assistance is optimized into 60 mg / kg, single injection and 22 DEG C normal-temperature culture, optimal parameters induced by busulfan are screened out through experiments, the operation process is simplified under the action of ensuring that endogenous germ cells of the acceptor are reduced and immunological rejection is reduced, and the method is suitable for large-scale popularization and application. The harsh requirement of a high-temperature environment is avoided, and meanwhile the field planting efficiency of donor cells in receptors and the survival rate of the receptors are improved.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

The invention relates to a method and a kit for generating nucleic acid for target capture. The method involves binding of a circ-olio sequence to at least one target oligonucleotide to generate a single stranded circular DNA. The single stranded circular DNA is bidirectionally amplified with the sense and antisense oligonucleotides, followed by biotinylation to obtain for a double-stranded concatemer of linear sequence. The double-stranded concatemer of linear sequence with the restriction endonucleases produces a target capture nucleic acid. The method offers a scalable targeted capture of pr eselected genomic regions, and is cost effective. The method of generating nucleic acid for target capture has application in the field of oncology hematology, inherited malignancies, germline disorders, microbiology and prenatal diagnosis. The invention generates baits over high GC-rich regions of the genome.

The invention discloses an AI-based human germ cell development and culture system evaluation method, which comprises the following steps: step 1, constructing a standardized reference map, step 2, establishing a germ cell development stage prediction model which comprises a transfer learning-based single cell annotation variation inference prediction module, a united prediction module combining single cell annotation variation inference with k nearest neighbor and a cell type classification module for feature selection optimization realize collaborative decision through a three-level confidence system; and step 3, in-vitro culture system evaluation, including construction of double indexes for quantitative evaluation and analysis of evaluation results. According to the method, a whole-process solution from single cell reference map construction, development stage prediction to culture system evaluation is realized, and the problems of reference data fragmentation, inaccurate development stage annotation and lack of quantitative standards of culture system data in the current germ cell research field are solved.