3 results about "Chemical mutagens" patented technology

The invention relates to the technical field of crop breeding, and discloses a rapid breeding method of high-dietary-fiber glutinous highland barley, which comprises the following steps: seed pretreatment, chemical mutagenesis treatment, culture solution preparation, greenhouse culture and early comprehensive screening, rapid genetic homozygosis and high-generation accurate phenotype selection. By preparing mutagenesis liquid with specific components to induce seeds, a chemical mutagenic agent contained in the mutagenesis liquid can initiate directional variation of genetic materials of the highland barley seeds, so that the genetic basis of high dietary fibers and waxy characters is widened, and rich variant groups are provided for subsequent screening; meanwhile, in the greenhouse culture and early comprehensive screening stage, the content of beta-glucan in seeds of a single plant is subjected to nondestructive measurement by utilizing a near infrared spectrum technology, so that high-throughput and low-cost quality primary screening on a huge group in the early generation of breeding is realized, and the bottlenecks that a traditional chemical detection method is long in period, high in cost and cannot be used for early generation selection are overcome; therefore, the screening efficiency of the germplasm resources is improved.

The present application relates to the field of agricultural technology, in particular to a plant temperature-sensitive male sterility gene mutant tms17 and application thereof.The nucleotide sequence of the plant temperature-sensitive male sterility gene mutant tms17 comprises the nucleotide sequence shown in SEQ ID NO.1 in the sequence listing.The present application screens a new temperature-sensitive male sterile line tms17 by chemical mutagen ethyl methyl sulfone (EMS) mutagenesis of japonica rice variety ZH11, which is completely sterile at high temperature and fertility is restored at low temperature, and the mechanism of temperature-sensitive fertility restoration is disclosed, which provides a new germplasm resource with application potential for rice two-line hybrid breeding.

The invention provides methods for mutagenizing plants or parts thereof, comprising subjecting a plant tissue to illumination with UV light followed by treatment with a chemical mutagen. The methods may advantageously be applied to methods of identifying a plant of a predefined species carrying one or more predetermined mutation(s) in nucleotide(s) of interest [NOI(s)], in a target sequence.