9 results about "Chemical mutagens" patented technology

The invention discloses a quinoa CqARF2 gene mutant as well as an identification method and application thereof. Chenopodium quinoa seeds are mutated on a large scale by utilizing a chemical mutagenic agent EMS, a CqARF2 gene function deletion mutant arf2 is adopted as a quinoa mutant, G at the 1050th site of the CqARF2 gene of the mutant is mutated into A, and amino acid at the 350th site is mutated into a termination codon, so that the translation process of CqARF2 protein is terminated in advance. The chenopodium quinoa CqARF2 gene function deletion mutant arf2 shows remarkable agronomic character improvement, seeds are large and full, compared with a wild type, the hundred-grain weight of the mutant seeds is increased by about 40%, the seed diameter is increased by about 10%, the seed thickness is increased by about 30%, and a certain foundation is laid for breeding new high-yield and high-quality chenopodium quinoa varieties.

The present invention provides a hexaploid Salvia miltiorrhiza and a cultivation method, which relate to the technical field of hexaploid Salvia miltiorrhiza cultivation, including chromosome manipulation technology. The hexaploid Salvia miltiorrhiza and the cultivation method include the following steps: Sp1, collecting diploid Salvia miltiorrhiza germplasm resources, collecting 6 materials from Yunnan, Sichuan, Shaanxi, Shandong, Hebei, Tianjin and other places, and establishing a Salvia miltiorrhiza germplasm resource material planting nursery; Sp2, performing morphological observation, trait analysis, chromosome ploidy identification and genome analysis on the Salvia miltiorrhiza germplasm resources, the results of which show that the somatic cells of white Salvia miltiorrhiza and purple Salvia miltiorrhiza are all diploid, that is, 2n=2x=16; Sp3, based on the identification results, selecting diploid white Salvia miltiorrhiza and artificially inducing tetraploid Salvia miltiorrhiza using a chemical mutagen. Taking the mutagenized white Salvia miltiorrhiza seedlings for chromosome ploidy identification, screening homozygous tetraploid white Salvia miltiorrhiza, and then performing triploid hybridization of the tetraploid white Salvia miltiorrhiza and the diploid purple Salvia miltiorrhiza.

The invention belongs to the technical field of vitamin B2 production, and particularly relates to a preparation method of a safe high-yield strain for vitamin B2 production, which comprises the following steps: S1, strain source and screening: analyzing the current situation and development trend of vitamin B2, and screening the strain; s2, genetic engineering modification: evaluating the production performance and genetic stability of the strain, and carrying out mutagenesis treatment on the screened strain by using a chemical mutagenic agent to obtain a genetically mutated strain; s3, screening and identifying high-yield mutant strains: integrating the fragments containing the spore cereus dry riboflavin operon into a vitamin B2 genome to generate the mutant strains, and performing fine adjustment on the riboflavin operon in the mutant strains by utilizing a multiple genome editing system. And through technical popularization, the manufacturing cost of the product can be reduced, and the social benefit is obvious.

The invention relates to the technical field of crop breeding, and discloses a rapid breeding method of high-dietary-fiber glutinous highland barley, which comprises the following steps: seed pretreatment, chemical mutagenesis treatment, culture solution preparation, greenhouse culture and early comprehensive screening, rapid genetic homozygosis and high-generation accurate phenotype selection. By preparing mutagenesis liquid with specific components to induce seeds, a chemical mutagenic agent contained in the mutagenesis liquid can initiate directional variation of genetic materials of the highland barley seeds, so that the genetic basis of high dietary fibers and waxy characters is widened, and rich variant groups are provided for subsequent screening; meanwhile, in the greenhouse culture and early comprehensive screening stage, the content of beta-glucan in seeds of a single plant is subjected to nondestructive measurement by utilizing a near infrared spectrum technology, so that high-throughput and low-cost quality primary screening on a huge group in the early generation of breeding is realized, and the bottlenecks that a traditional chemical detection method is long in period, high in cost and cannot be used for early generation selection are overcome; therefore, the screening efficiency of the germplasm resources is improved.

The invention belongs to the technical field of bamboo reed breeding, and discloses a mutation breeding method for obtaining a bamboo reed cold-resistant mutant based on EMS (Enhanced Mass Spectrometry), which comprises the following steps: step 1, inducing embryogenic callus; step 2, carrying out EMS mutagenesis treatment; step 3, performing EMS stress screening; 4, differentiation and rooting culture of adventitious buds; 5, screening the tissue culture seedlings at low temperature; the mutation obtained through mutagenesis treatment is a permanent change of a genetic structure of an organism and may appear in germ cells and somatic cells of a plant. Physical or chemical mutagenic agents are used to increase the frequency of mutations. In chemical mutagens, EMS is considered to be the most effective mutagen, the EMS can generate a large number of non-lethal point mutations in the whole genome, the mutation frequency is high, the chromosome abnormality frequency is low, the EMS is used for treating embryogenic calluses of the bamboo reeds, Hyp is used for screening, the low-temperature-resistant bamboo reed mutants are obtained, the planting area of the bamboo reeds can be enlarged, and the survival rate of the bamboo reeds is increased. And waste land in a low-temperature region is fully utilized.

The present application relates to the field of agricultural technology, in particular to a plant temperature-sensitive male sterility gene mutant tms17 and application thereof.The nucleotide sequence of the plant temperature-sensitive male sterility gene mutant tms17 comprises the nucleotide sequence shown in SEQ ID NO.1 in the sequence listing.The present application screens a new temperature-sensitive male sterile line tms17 by chemical mutagen ethyl methyl sulfone (EMS) mutagenesis of japonica rice variety ZH11, which is completely sterile at high temperature and fertility is restored at low temperature, and the mechanism of temperature-sensitive fertility restoration is disclosed, which provides a new germplasm resource with application potential for rice two-line hybrid breeding.

The utility model discloses a device for efficient and safe EMS treatment to mutate rice seeds, which relates to the technical field of seed mutation, and comprises a soaking box, a seed soaking frame, a shaking mechanism and a spraying and washing mechanism, the soaking box is divided into an EMS soaking area and a clear water soaking area, the EMS soaking area and the clear water soaking area are separated by a middle arched partition plate, and the spraying and washing mechanism is arranged on the seed soaking frame. The center of the soaking box is rotationally sleeved with a center rotating shaft located above the arched partition plate, when rice seeds are suddenly soaked, automatic position changing of the seed soaking frame in a clear water soaking area and an EMS soaking area can be achieved through a stepping motor and a rotating frame, the seed soaking frame can further automatically move to the position below the spraying and flushing mechanism to be automatically flushed, and the rice seed soaking efficiency is improved. According to the seed treatment device, the residual chemical mutagenic agent is effectively removed, the purity of seeds is guaranteed, manual operation is reduced through automatic control, the seed treatment efficiency is greatly improved, the chance that operators make direct contact with the chemical mutagenic agent is reduced, and the safety risk in the operation process is reduced.

The invention belongs to the technical field of rape breeding, and particularly relates to a breeding method of high-oil rape germplasm, the high-oil rape germplasm is obtained by breeding rape parents after physical mutagenesis and chemical mutagenesis treatment, chemical mutagenesis uses a chemical mutagenic agent prepared from EMS, MNU and NU7026, physical mutagenesis adopts low-energy nitrogen ion beams or gamma rays, and chemical mutagenesis adopts low-energy nitrogen ion beams or gamma rays. By utilizing a compound mutagenesis method, the stability of gene mutation is improved, the heritable variation spectrum is greatly widened, the time required from mutagenesis to obtaining of new materials with stable characters is shortened, and the purpose of rapid breeding of high-oil rape germplasm is achieved.

The invention provides methods for mutagenizing plants or parts thereof, comprising subjecting a plant tissue to illumination with UV light followed by treatment with a chemical mutagen. The methods may advantageously be applied to methods of identifying a plant of a predefined species carrying one or more predetermined mutation(s) in nucleotide(s) of interest [NOI(s)], in a target sequence.