Method for screening yellow wine yeast of high-yield urea

A screening method and rice wine yeast technology are applied in the preparation of alcoholic beverages, fungi, etc., which can solve the problems of secondary pollution and increase the safety risk of alcohol, and achieve the effect of broad promotion and application prospects.

Active Publication Date: 2009-10-07
ZHEJIANG HONGSHILIANG GRP JIGONGJIA WINERY CO LTD
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Since the urine enzyme additive contains a large amount of impurities, addi...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select the rice wine yeast isolated from the beer company to carry out mutagenesis:

[0022] 1. Mutagenesis method:

[0023] 1) Activation of the starting strain: the strain HJ-4 was inoculated in the rice mash culture liquid with an addition amount of 3%, and cultured on a shaking table at 30°C for 18 hours until the logarithmic growth phase of the yeast, at which time the cell viability was the best, suitable for mutagenesis.

[0024] 2) Cell suspension preparation: centrifuge the activated bacterial solution at 3500r / min for 10min, collect the bacterial cells, wash once with sterilized normal saline, oscillate with a vortex mixer for 10min, count the number of bacterial cells with a hemocytometer, and then use Physiological saline was used to adjust the cell concentration to make it a bacterial suspension of 106 cells / ml.

[0025] 3) He-Ne laser mutagenesis: He-Ne laser mutagenesis was carried out by the test tube method: 200 μl each of the suspensions prepared abo...

Embodiment 2

[0037] The HJ-17 strain was selected for mutagenesis; the mutagenesis and screening methods were the same as in Example 1, and 3 strains with low urea production were obtained by isolation, screening, and identification, and the urea production was 15-20 times lower than that of the original strain.

Embodiment 3

[0039] Carry out mutagenesis to the HJ1615 strain; the mutagenesis and screening method are the same as in Example 1, and two strains with urea production 10-15 times lower than that of the parent strain can be screened and isolated, among which one strain HJ1615-D4 retains the original high alcohol content Tolerance performance, preserved to the China Microorganism Culture Collection Management Committee, the preservation number is: CGMCC No.2858.

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Abstract

The present invention discloses a method for screening yellow wine yeast of high-yield urea. The method can screen out the yeast colony with low urea yield from the yellow wine yeast mutagenized by chemical mutagen with a canavanine plate and can also screen out the yeast colony with better low-yield property from the yellow wine yeast colony of common low-yield urea. The method for screening according to the invention can quick separate and obtain the target mycopremna. The blindness of breeding process is reduced and the predictability is increased. The breeding efficiency is greatly increased. The yellow wine yeast obtained through screening has the characteristic of low content of byproduct urea and can effectively reduce the content of urethane in the production of yellow wine.

Description

technical field [0001] The invention belongs to the field of biology and fermentation engineering. It relates to a screening method for rice wine yeast with low urea production, which can quickly isolate and obtain the target strain, so that the low urea production strain can be quickly separated from a large number of treated cells, which reduces the blindness of the selection process, enhances predictability, and greatly improved breeding efficiency. Background technique [0002] Ethyl Carbamate (EC), with genotoxicity and carcinogenicity, is a possible human carcinogen (category 2A) confirmed by the International Agency for Research on Cancer (IARC). Since 2002, ethyl carbamate has become one of the key monitoring substances of the World Health Organization. However, due to various reasons, my country has not yet formulated a limit standard for EC, resulting in rice wine. The problem of excessive EC content in fermented wine such as wine and wine is very prominent. With t...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12G3/02
Inventor 王德良宋绪磊张彦青李红王异静
Owner ZHEJIANG HONGSHILIANG GRP JIGONGJIA WINERY CO LTD
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