18 results about "Mutagen" patented technology

The invention discloses a quinoa CqARF2 gene mutant as well as an identification method and application thereof. Chenopodium quinoa seeds are mutated on a large scale by utilizing a chemical mutagenic agent EMS, a CqARF2 gene function deletion mutant arf2 is adopted as a quinoa mutant, G at the 1050th site of the CqARF2 gene of the mutant is mutated into A, and amino acid at the 350th site is mutated into a termination codon, so that the translation process of CqARF2 protein is terminated in advance. The chenopodium quinoa CqARF2 gene function deletion mutant arf2 shows remarkable agronomic character improvement, seeds are large and full, compared with a wild type, the hundred-grain weight of the mutant seeds is increased by about 40%, the seed diameter is increased by about 10%, the seed thickness is increased by about 30%, and a certain foundation is laid for breeding new high-yield and high-quality chenopodium quinoa varieties.

The present invention provides a hexaploid Salvia miltiorrhiza and a cultivation method, which relate to the technical field of hexaploid Salvia miltiorrhiza cultivation, including chromosome manipulation technology. The hexaploid Salvia miltiorrhiza and the cultivation method include the following steps: Sp1, collecting diploid Salvia miltiorrhiza germplasm resources, collecting 6 materials from Yunnan, Sichuan, Shaanxi, Shandong, Hebei, Tianjin and other places, and establishing a Salvia miltiorrhiza germplasm resource material planting nursery; Sp2, performing morphological observation, trait analysis, chromosome ploidy identification and genome analysis on the Salvia miltiorrhiza germplasm resources, the results of which show that the somatic cells of white Salvia miltiorrhiza and purple Salvia miltiorrhiza are all diploid, that is, 2n=2x=16; Sp3, based on the identification results, selecting diploid white Salvia miltiorrhiza and artificially inducing tetraploid Salvia miltiorrhiza using a chemical mutagen. Taking the mutagenized white Salvia miltiorrhiza seedlings for chromosome ploidy identification, screening homozygous tetraploid white Salvia miltiorrhiza, and then performing triploid hybridization of the tetraploid white Salvia miltiorrhiza and the diploid purple Salvia miltiorrhiza.

The invention relates to the technical field of crop breeding, and discloses a rapid breeding method of high-dietary-fiber glutinous highland barley, which comprises the following steps: seed pretreatment, chemical mutagenesis treatment, culture solution preparation, greenhouse culture and early comprehensive screening, rapid genetic homozygosis and high-generation accurate phenotype selection. By preparing mutagenesis liquid with specific components to induce seeds, a chemical mutagenic agent contained in the mutagenesis liquid can initiate directional variation of genetic materials of the highland barley seeds, so that the genetic basis of high dietary fibers and waxy characters is widened, and rich variant groups are provided for subsequent screening; meanwhile, in the greenhouse culture and early comprehensive screening stage, the content of beta-glucan in seeds of a single plant is subjected to nondestructive measurement by utilizing a near infrared spectrum technology, so that high-throughput and low-cost quality primary screening on a huge group in the early generation of breeding is realized, and the bottlenecks that a traditional chemical detection method is long in period, high in cost and cannot be used for early generation selection are overcome; therefore, the screening efficiency of the germplasm resources is improved.

The invention belongs to the technical field of bamboo reed breeding, and discloses a mutation breeding method for obtaining a bamboo reed cold-resistant mutant based on EMS (Enhanced Mass Spectrometry), which comprises the following steps: step 1, inducing embryogenic callus; step 2, carrying out EMS mutagenesis treatment; step 3, performing EMS stress screening; 4, differentiation and rooting culture of adventitious buds; 5, screening the tissue culture seedlings at low temperature; the mutation obtained through mutagenesis treatment is a permanent change of a genetic structure of an organism and may appear in germ cells and somatic cells of a plant. Physical or chemical mutagenic agents are used to increase the frequency of mutations. In chemical mutagens, EMS is considered to be the most effective mutagen, the EMS can generate a large number of non-lethal point mutations in the whole genome, the mutation frequency is high, the chromosome abnormality frequency is low, the EMS is used for treating embryogenic calluses of the bamboo reeds, Hyp is used for screening, the low-temperature-resistant bamboo reed mutants are obtained, the planting area of the bamboo reeds can be enlarged, and the survival rate of the bamboo reeds is increased. And waste land in a low-temperature region is fully utilized.

The present application relates to the field of agricultural technology, in particular to a plant temperature-sensitive male sterility gene mutant tms17 and application thereof.The nucleotide sequence of the plant temperature-sensitive male sterility gene mutant tms17 comprises the nucleotide sequence shown in SEQ ID NO.1 in the sequence listing.The present application screens a new temperature-sensitive male sterile line tms17 by chemical mutagen ethyl methyl sulfone (EMS) mutagenesis of japonica rice variety ZH11, which is completely sterile at high temperature and fertility is restored at low temperature, and the mechanism of temperature-sensitive fertility restoration is disclosed, which provides a new germplasm resource with application potential for rice two-line hybrid breeding.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

The present invention provides a new flavonoid compound and its preparation method and application, belonging to the field of medical technology. The flavonoid compound is isolated from the Calophyllum membranaceum Gardn.et Champ. of the Guttiferae family. The dried stems and leaves of Calophyllum membranaceum are taken, and a new flavonoid compound is obtained by extract extraction, MCI segmentation decolorization, silica gel column chromatography, open ODS column chromatography, Sephadex LH-20 gel column chromatography, and semi-preparative HPLC chromatography: the preparation method of the present invention is simple to operate and has a high degree of purification. The flavonoid compound of the present invention can target and inhibit CYP1A1 enzyme activity, can be used as a lead compound for developing drugs that inhibit CYP1A1 enzyme activity, is expected to prevent or treat cancers caused by pro-carcinogens / mutagens such as polycyclic aromatic hydrocarbons and heterocyclic aromatic amines, and has potential clinical application value in the development of drugs such as anti-lung cancer, anti-breast cancer, and anti-colon cancer.

The invention relates to the field of microbial technology and genetic breeding, and discloses a mutagen-assisted adaptive laboratory evolution method and application, the mutagen-assisted adaptive laboratory evolution method comprises the following steps: A1, mutagenesis; a2, carrying out passage; a3, continuous mutagenesis and passage are carried out, and during each repetition, preset adaptive conditions are gradually improved according to a preset amplification until a target culture is screened out, according to the method, an efficient mutagenesis mechanism and a continuous directed evolution process are subjected to in-situ and deep integration, so that high-frequency and directed adaptive evolution of microbial genotypes under strong selection pressure is realized, and the efficiency is improved.

The invention belongs to the technical field of plant cell engineering, and particularly relates to a culture method of a hormone autotrophic ginseng cell line and the ginseng cell line. The culture method comprises the following steps: peeling, cleaning and disinfecting a ginseng embryo, slicing, inoculating to an MS solid culture medium, and inducing to obtain an embryogenic cell line; transferring to an MS liquid culture medium for suspension culture to obtain a stable suspension cell line; carrying out nursing culture after treatment with a composite mutagenic agent; and transferring the growing cell cluster to a hormone-free MS solid culture medium for multiple subculture, and screening out a cell line independent of exogenous auxin. The cell line can proliferate without exogenous hormones, the residual risk is eliminated, the cost is reduced, and the cell line is suitable for the fields of food and medicine and has important application value.

The present application belongs to the technical field of new plant variety breeding, and particularly relates to a breeding method of a bletilla triplod and application thereof. The breeding method of the bletilla triplod comprises the following steps: screening a bletilla diploid; artificially inducing and identifying a bletilla tetraploid; culturing the identified bletilla tetraploid to flowering; and hybridizing the bletilla tetraploid and the bletilla diploid to obtain a bletilla triplod fruit; wherein a mutagen used for the induction is a mixed aqueous solution of 10 ppm p-dichlorobenzene and 5 ppm alpha-bromonaphthalene. The present application first creates a new bletilla triplod variety, and establishes a complete set of bletilla triplod advantage utilization and breeding method. The present application first obtains a new bletilla triplod germplasm, and lays a scientific foundation for bletilla triplod breeding.

The current invention pertains to a use of a scavenger in method or protocol for treatment of a plant cell with a mutagen, optionally in a method for random mutagenesis, mutagenized plants obtained using said method or protocol, and a composition or kit for use in said method or protocol.

The invention relates to the technical field of gene engineering, and discloses a method for detecting chemical mutants by utilizing zebra fish reverse mutation and application of the method. After an actb2 gene promoter is obtained by utilizing a Tol2 transposon system, a transgenic reporter plasmid for expressing EGFP-mutant is constructed; eNU and DEN are used for verifying the feasibility of application of the method in detection of mutagenic compounds. The method is convenient, feasible and effective, and has the advantages of being low in cost and suitable for high-throughput screening compared with other models such as mice.

The invention provides a polypeptide for promoting synthesis of plant anthocyanin and application, and belongs to the technical field of genetic engineering. The invention provides a polypeptide for promoting the synthesis of plant anthocyanin. The polypeptide is characterized in that a key site of a key structural domain for regulating and controlling the synthesis of the anthocyanin is mutated. According to the invention, the existing sequence is subjected to key site mutation and optimization, and is used for promoting the biosynthesis of anthocyanin. By mutating the key amino acid site of the original sequence, the regulation and control capability of the transcription factor in anthocyanin biosynthesis can be obviously improved. The invention also constructs an overexpression vector based on the coding gene or the optimized gene of the polypeptide, and the anthocyanin accumulation amount of a transgenic material can be obviously promoted by overexpressing the gene or the optimized gene in a plant cell.

The invention relates to the technical field of tetramethylpyrazine strain breeding, in particular to a tetramethylpyrazine strain high-throughput breeding device. Comprising a breeding box; a door plate is rotatably mounted on the breeding box, a handle is fixedly connected to the side wall of the door plate, a magnetic strip is embedded in the upper portion of the breeding box, a control panel is mounted on the side wall of the breeding box, ultraviolet lamps which are symmetrically distributed are mounted on the side wall in the breeding box, and a mounting frame is mounted at the bottom in the breeding box. And a mounting groove is formed in the mounting frame. The adding amount of nitrosoguanidine is accurately controlled through the quantitative assembly, the concentration and dosage of a mutagenic agent fed into each adding tank are consistent, the problem that the adding amount of nitrosoguanidine in traditional chemical mutagenesis is difficult to control in a standardized mode is solved, the difference of mutagenesis effects of strains in different batches is effectively avoided, the stability of the positive mutation rate of the strains is guaranteed, and the yield of the strains is increased. And the reliability of chemical mutagenesis is improved.

The application discloses a method for constructing a large fragment deletion mutant library of Magnaporthe oryzae, and belongs to the field of agricultural biotechnology.The method comprises the step of adding DEB to the protoplast of the Magnaporthe oryzae for culture and inducing the production of conidia.The application utilizes the chemical mutagen DEB to obtain the Magnaporthe oryzae mutant at a high frequency on a whole genome scale, and provides a new technical path for the functional genomics research of the Magnaporthe oryzae.The method provided by the application is helpful to systematically analyze the pathogenic mechanism, the infection process and the environmental adaptation regulation network of the Magnaporthe oryzae, and provides support for mining new disease-resistant targets and disease-resistant mechanisms.The method has the advantages of high efficiency, low cost and short cycle, and has wide application potential in the genetic research of plant pathogenic fungi.

The present invention relates to a method for leaching vanadium from vanadium shale by acclimating and mutagenizing bacteria with a vanadium solution. The technical scheme comprises the following steps: calcining ground vanadium shale at 600-850°C to obtain a decarbonized vanadium shale powder, which is then added to a nitrogen-containing culture medium to produce a bioleaching medium. A bacterial solution of Bacillus mucilaginosus is then mixed with the nitrogen-containing culture medium, followed by multiple chemical mutagenesis cultures with the addition of a mutagen to produce a mutagenized Bacillus mucilaginosus culture. The mutagenized Bacillus mucilaginosus culture is then mixed with the nitrogen-containing culture medium and then acclimated and cultured with a vanadium solution to produce an acclimated mutagenized Bacillus mucilaginosus culture. The acclimated mutagenized Bacillus mucilaginosus culture is then mixed with the bioleaching medium and leached for 20-25 days to produce a vanadium shale bioleaching slurry. Solid-liquid separation is then performed to produce a vanadium-containing leachate. The vanadium leaching rate is 70.36-84.08%. The acclimated mutagenized Bacillus mucilaginosus has enhanced growth and metabolic capacity, shortens the leaching cycle, and improves the leaching efficiency of vanadium from vanadium shale.

The invention provides methods for mutagenizing plants or parts thereof, comprising subjecting a plant tissue to illumination with UV light followed by treatment with a chemical mutagen. The methods may advantageously be applied to methods of identifying a plant of a predefined species carrying one or more predetermined mutation(s) in nucleotide(s) of interest [NOI(s)], in a target sequence.

This application belongs to the field of breeding technology and specifically provides a method for mutagenesis breeding of Porphyra haitanensis, comprising the following steps: 1) irradiating the coniferous bodies of Porphyra haitanensis with ultraviolet light; 2) treating the irradiated coniferous bodies with a mutagen comprising nitrosoguanidine and a slow-release solution comprising bleomycin and bleomycin; and 3) screening the mutagenized coniferous bodies to obtain mutants, which are then cultured to obtain thallus. This breeding method has the advantages of high efficiency, high mutation rate, and good specificity.