13 results about "Early generation" patented technology

The present application relates to the field of biotechnology and molecular breeding technology, and provides a KASP molecular marker linked to a wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 and an application thereof, wherein the physical position of the SNP site of the KASP molecular marker linked to the wheat-ice grass distant hybrid offspring powdery mildew resistance site PmYZW-6A-2 corresponds to the position 95180338 bp on the 6A chromosome of the Puchun 3504 genome; the nucleotide sequence of the SNP site is shown as SEQ ID NO. 4, and M in the nucleotide sequence is A or G. Through the above technical solution, the problems of long screening period and large workload of wheat-ice grass distant hybrid offspring powdery mildew resistance, inability to accurately screen in early generations, and difficult breeding in the related art are solved.

The invention discloses a genetic improvement method and a matched cultivation method of a kidney bean variety suitable for machine harvesting, and relates to the technical field of kidney bean planting. Through a molecular marker-assisted selection technology, key character gene loci such as pod cracking resistance and plant uprightness are accurately positioned, rapid screening of target genotypes is realized in early generations of hybridization, the breeding period is shortened by more than 40% compared with a traditional breeding period, and the breeding efficiency is remarkably improved; the bred variety has the characteristics of high pod cracking resistance, upright and lodging-resistant plants, concentrated pod bearing of main stems, good maturing consistency and the like, so that the working efficiency of the combine harvester is improved to 95% or above, the impurity rate is controlled within 3%, and the bottleneck problem of mechanical harvesting is effectively solved; through combination of deep scarification soil preparation and precision sowing, root system development and a population structure are optimized; a drip irrigation system and a dynamic fertilization strategy ensure accurate supply of water and fertilizer in a flower and pod period, and the plumpness of pods is improved by 15-20%; the growth regulator regulates the plant height to 2-3 m, and the lodging resistance is enhanced.

The invention relates to the technical field of crop breeding, and discloses a rapid breeding method of high-dietary-fiber glutinous highland barley, which comprises the following steps: seed pretreatment, chemical mutagenesis treatment, culture solution preparation, greenhouse culture and early comprehensive screening, rapid genetic homozygosis and high-generation accurate phenotype selection. By preparing mutagenesis liquid with specific components to induce seeds, a chemical mutagenic agent contained in the mutagenesis liquid can initiate directional variation of genetic materials of the highland barley seeds, so that the genetic basis of high dietary fibers and waxy characters is widened, and rich variant groups are provided for subsequent screening; meanwhile, in the greenhouse culture and early comprehensive screening stage, the content of beta-glucan in seeds of a single plant is subjected to nondestructive measurement by utilizing a near infrared spectrum technology, so that high-throughput and low-cost quality primary screening on a huge group in the early generation of breeding is realized, and the bottlenecks that a traditional chemical detection method is long in period, high in cost and cannot be used for early generation selection are overcome; therefore, the screening efficiency of the germplasm resources is improved.

This invention relates to a GmSPL3b gene SNP marker and its application, belonging to the fields of plant molecular biology and crop genetics and breeding technology. The SNP marker is used for molecular detection of soybean flowering and maturity stages. The SNP is a single nucleotide mutation at position 94 and / or position 116 of the first exon of the soybean GmSPL3b gene, forming a nucleotide sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 2; the polymorphism of the single nucleotide mutation is A / G polymorphism. This invention enables precise genotyping at the seedling stage, efficiently breeding early-maturing soybean varieties adapted to high-latitude regions. It can directly serve as a precise target for gene editing, providing a clear basis for molecular design breeding. It allows for rapid screening of ideal genotypes in early generations, reducing field workload, shortening the breeding cycle, and accelerating the selection of new varieties.

The invention discloses a breeding method and application of fragrant soft rice three-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during parent selection of high-quality hybrid rice is solved for the first time, consistency of the taste quality and the control genes thereof of the hybridized and matched parents is ensured, and the breeding cost is reduced; synchronous phenotype polymerization selection is carried out on an off-white endosperm marker gene representing excellent taste quality and a high combining ability, aromatic flavor and maintenance gene, so that the problem that taste quality selection is difficult to carry out on a high-quality hybridization maintenance single plant in a separation early generation is solved for the first time; in addition, soft rice marker genes with recessive grey-white endosperm are synchronously introduced into three-line hybrid rice parents, through main target character aggregation response identification, the fragrant soft rice three-line hybrid rice with grey-white markers in rice grains is successfully created for the first time, the yield is increased, and consumers can conveniently identify the authenticity of products and make brands.

The invention belongs to the technical field of crop stress resistance detection, and particularly relates to a wheat resistance evaluation method based on chloroplast and mitochondrial morphological quantitative indexes and application. The physiological index chloroplast number, the mitochondrial cross section and the ratio of the mitochondrial matrix area to the ridge area selected by the evaluation method can directly reflect the physiological adaptability of wheat to high temperature and drought stress, are not interfered by environmental factors, and have higher specificity and accuracy; the method also has the characteristics of short detection period and high efficiency, the sample preparation and detection process can be completed within 7-10 days, and stress resistance screening of a large number of germplasm resources can be rapidly realized; the operation process of the evaluation method is standard, the interference of human factors can be effectively avoided, and the repeatability and the stability of a detection result are good; the method can be suitable for high-temperature drought resistance identification of different types of wheat varieties, can also be used for early generation screening in the wheat stress-resistant breeding process, and provides reliable technical support for cultivation and popularization of the stress-resistant wheat varieties.

The application provides a kind of based on indica japonica hybrid assorting and early generation regeneration force directional screening's ratoon rice breeding method, belongs to rice breeding technical field.A kind of based on indica japonica hybrid assorting and early generation regeneration force directional screening's ratoon rice breeding method, constructs " indica japonica intermediate type x strong ratoon rice" three hybrid system is screened after the first season harvest, obtains the strain of sprouting fast, ratoon shoot is more, after the screening of recovery rate, accumulated temperature loss and yield, obtains a kind of ratoon rice strain.The breeding cycle of the method is shortened by 3-4 years compared with the traditional method, and the screened ratoon rice strain has the characteristics of strong regeneration capacity, high yield and lodging resistance, and can realize "one-time sowing two-season mechanical harvesting" full-process mechanized production.

The application discloses a primer pair related to wheat scab resistance and application, the nucleotide sequence of the primer pair is shown as SEQ ID NO.1 and SEQ ID NO.2, and the primer pair can be used to detect wheat scab resistance; namely, taking wheat genome DNA as a template, performing PCR amplification on the template by using the primer pair, performing electrophoresis on the amplification product on a 1% agarose gel to obtain a detection result, and if the amplification product contains a 154bp band, it is indicated that the wheat material contains an excellent allelic variation based on T, and the wheat material has scab resistance; and the method can be applied to screening of the wheat resistant to scab and selection of early generations in breeding.

The invention relates to the technical field of biotechnology and molecular breeding, and provides a KASP molecular marker linked with a powdery mildew resistance site PmYZW-6A-2 of wheat-agropyron cristatum distant filial generation and application of the KASP molecular marker. The physical position of an SNP (Single Nucleotide Polymorphism) site of the KASP molecular marker linked with the powdery mildew resistance site PmYZW-6A-2 of the wheat-agropyron cristatum distant filial generation corresponds to the 95180338th bp position on the 6A chromosome of the Price 3504 genome; the nucleotide sequence of the SNP site is as shown in SEQ ID NO.4, and M in the nucleotide sequence is A or G. According to the technical scheme, the problems that the wheat-wheatgrass distant filial generation powdery mildew resistance screening period is long, the workload is large, accurate screening cannot be conducted in early generations, and the breeding difficulty is large in the prior art are solved.

The application belongs to the field of molecular biology, and particularly relates to a wheat kernel circumference and thousand kernel weight related KASP marker and application thereof. The specific technical scheme is as follows: application of a KASP marker in wheat breeding, the KASP marker is located at 386292170bp of wheat 3D chromosome, and the base at the 386292170th position is A or G. The application provides an efficient, simple and reliable reference marker for screening of high-grain-weight and high-yield wheat, and the screening of excellent genotype materials can be completed through genotyping detection in early generations or seedling stages when kernels have not yet been formed, and the efficiency of wheat high-yield genetic improvement is effectively improved.

The invention relates to the technical field of crop breeding, and discloses an efficient breeding method for directionally improving comprehensive characters of winter wheat, which comprises the following steps: step (1) cross parent selection and preliminary gene polymerization; (2) early generation gene pool construction and high-pressure disease resistance screening; (3) high generation selection and homozygous acceleration; (4) identifying the eurytopic adaptability of multiple ecological regions; and (5) carrying out directional selection on agronomic characters in the whole growth period. According to the method, a single-spike continuous selection strategy is adopted in F2-F4 generations, compared with traditional single plant selection, more inter-plant variation can be contained, gene differences in the same plant can be reserved, a breeding material gene pool is enriched, recessive excellent gene loss caused by only paying attention to external forms is avoided through collaborative screening of agronomic phenotypic characters, early stress resistance and quality preliminary screening, and the breeding quality is improved. The polymerization probability of high-yield, high-quality, disease-resistant and other target genes is remarkably improved, the pain point of waste of early generation gene resources in the traditional breeding technology is effectively solved, and a solid gene foundation is laid for multi-character collaborative improvement; f5 and above ear row / single plant rapid homozygous stable new materials and guarantee the uniformity of offspring, the breeding efficiency can be improved, and provincial and above regional tests can be participated 2-3 years in advance.

The present application belongs to the field of plant biotechnology and molecular breeding technology, and particularly relates to a drought resistance early identification method for macadamia based on multi-omics markers and application thereof. The present application detects the expression amount of MiWRKY22, MiNAC29 and MiP5CS genes in the leaves of the macadamia to be tested, and the content of proline and quercetin, substitutes the detection data into a drought resistance index (DRI) calculation model, and determines the drought resistance grade of the plant according to the DRI value. The present application integrates transcription and metabolic information into a quantifiable evaluation index for the first time, can be identified under non-stress conditions at the seedling stage, has the advantages of early identification, accuracy and high throughput, is suitable for macadamia germplasm resource evaluation, parent selection and early generation screening of breeding, and significantly improves the drought resistance breeding efficiency.

The invention discloses a primer pair related to wheat scab resistance and application, nucleotide sequences of the primer pair are shown as SEQ ID NO.1 and SEQ ID NO.2, and the primer pair can be used for detecting the wheat scab resistance. Wheat genome DNA is used as a template, the primer pair is used for PCR amplification, an amplification product is subjected to electrophoresis on 1% agarose gel to obtain a detection result, and if the amplification product contains a 154bp band, it is indicated that the wheat material contains excellent allelic variation resistant to gibberellic disease based on T and has gibberellic disease resistance; the method can be applied to screening of wheat resistant to gibberellic disease and selection of early generations of breeding.