Closely linked molecular marker for novel gene (SS2) of rice sink source

Abstract

The invention relates to a closely linked molecular marker for a novel gene (SS2) of a rice sink source and belongs to the fields of super-high-yield breeding of rice and molecular genetics. The invention aims at constructing a set of contig system of a target segment by using an F2 secondary separation group constructed by selfing a near-isogenic line carrying an SS2 heterozygous segment as a testing piece according to the linked separation rule; high-precision linked analysis for a target gene is carried out by combining with a phenotype; after two-point repeated identification for two years, the SS2 is finally and finely positioned in an interval of 104Kb on the 3rd chromosome, and a molecular marker SL14 based on a PCR (Polymerase Chain Reaction) closely linked with the SS2 is obtained. The closely linked molecular marker disclosed by the invention is applied to super-high-yield breeding of the rice, so that a genotype individium for simultaneously influencing the sink (the numberof grain per ear) and source (the length of sword leaves) characters can be quickly and accurately identified; the early generation selection of a breeding material is carried out; and the progress of the super-high-yield breeding of the rice is accelerated.

Description

technical field [0001] The invention relates to a tightly linked molecular marker of a new gene SS2 for controlling rice sink (number of grains per panicle) source (flag leaf size), which belongs to the fields of super-high-yield rice breeding and molecular genetics, and is suitable for introducing new genes in rice high-yield breeding Yield-related gene SS2, and closely linked molecular markers were used for assisted selection breeding of rice sink-source related traits. Background technique [0002] From the analysis of rice breeding history in my country, the balance of source, sink and flow has played an important role in the improvement of rice yield potential. Regardless of the dwarf breeding in the 1950s, the hybrid rice breeding in the 1970s, or the super rice breeding in recent years, every major increase in rice yield is accompanied by the improvement of source, sink, and flow conditions and the establishment of a new source-sink balance relationship. Establish. ...

AI Technical Summary

Problems solved by technology

Although the source traits such as leaf length and leaf size are easy to be routinely selected, conventional selection cannot distinguish the different gene loci that affect the source traits, and only through marker-assisted, targeted selection of genes that affect the sink-source traits (i.e. the above-mentioned first In order to quickly and effectively select and breed ultra-high-yielding varieties with coordinated pools and sources

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