Closely linked molecular marker for novel gene (SS2) of rice sink source

A technology of molecular markers and genes, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of inability to distinguish different gene loci

Inactive Publication Date: 2012-05-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Although the source traits such as leaf length and leaf size are easy to be routinely selected, conventional selection cannot distinguish the different gene loci that affect the source traits, and only through marker-assisted, targeted selection of genes that affect the sink-source traits (i.e. the above-mentioned first In order to quickly and effectively select and breed ultra-high-yielding varieties with coordinated pools and sources

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  • Closely linked molecular marker for novel gene (SS2) of rice sink source
  • Closely linked molecular marker for novel gene (SS2) of rice sink source
  • Closely linked molecular marker for novel gene (SS2) of rice sink source

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified.

[0011] (1) Preliminary mapping of new genes controlling sink-source in rice

[0012] 1. Test materials

[0013] The two-way backcross introduction line constructed by the high-quality japonica rice variety Lemont in the southern United States and the high-yielding indica rice variety Teqing in Guangdong, my country was used as the main research materials. Its construction process is as follows: the hybrid F of Lemont and Teqing 1 , F 1 The plants were backcrossed with their parents respectively to form two-way backcross BCs of Lemont and Teqing backgrounds 1 f 1 In the population, randomly select individual plants from the bidirectional backcross progeny and carry out 2-4 consecutive backcrosses with their respective recurrent parents, and stabilize them through multiple generation...

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Abstract

The invention relates to a closely linked molecular marker for a novel gene (SS2) of a rice sink source and belongs to the fields of super-high-yield breeding of rice and molecular genetics. The invention aims at constructing a set of contig system of a target segment by using an F2 secondary separation group constructed by selfing a near-isogenic line carrying an SS2 heterozygous segment as a testing piece according to the linked separation rule; high-precision linked analysis for a target gene is carried out by combining with a phenotype; after two-point repeated identification for two years, the SS2 is finally and finely positioned in an interval of 104Kb on the 3rd chromosome, and a molecular marker SL14 based on a PCR (Polymerase Chain Reaction) closely linked with the SS2 is obtained. The closely linked molecular marker disclosed by the invention is applied to super-high-yield breeding of the rice, so that a genotype individium for simultaneously influencing the sink (the numberof grain per ear) and source (the length of sword leaves) characters can be quickly and accurately identified; the early generation selection of a breeding material is carried out; and the progress of the super-high-yield breeding of the rice is accelerated.

Description

technical field [0001] The invention relates to a tightly linked molecular marker of a new gene SS2 for controlling rice sink (number of grains per panicle) source (flag leaf size), which belongs to the fields of super-high-yield rice breeding and molecular genetics, and is suitable for introducing new genes in rice high-yield breeding Yield-related gene SS2, and closely linked molecular markers were used for assisted selection breeding of rice sink-source related traits. Background technique [0002] From the analysis of rice breeding history in my country, the balance of source, sink and flow has played an important role in the improvement of rice yield potential. Regardless of the dwarf breeding in the 1950s, the hybrid rice breeding in the 1970s, or the super rice breeding in recent years, every major increase in rice yield is accompanied by the improvement of source, sink, and flow conditions and the establishment of a new source-sink balance relationship. Establish. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 徐建龙王韵黎志康孙勇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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