The invention discloses a preparation method for a mature vinegar. The method comprises the following steps: A, preparing raw materials: mixing fried hulls, fried wheat bran, liquid of fried rice, water, glucose powders and yeast extract, followed by a sterilizing, then adding edible alcohol and acetic acid bacteria to obtain the mixed raw materials; B, carrying out a fermenting: carrying out a fermenting for the mixed raw materials through a continuous ventilation stirring of deep solid-liquid state substance to obtain a solid-liquid state mature vinegar mash; C, carrying out a enzymolysis: adding first soy and a enzyme preparation to the solid-liquid state mature vinegar mash, and carrying out a solid-liquid state enzymolysis to obtain a enzymolysis vinegar mash; D, carrying out a post processing: sequential carrying out a heating for sterilize, a storing, a rough filtering, a fine filtering, a blending, a disinfecting and a sterilizing for the enzymolysis vinegar mash to obtain a finished product of the mature vinegar. The preparation method provided by the present invention has advantages of available raw materials, short fermentation period, easy realization of mechanized operation, high labour efficiency, low work intensity, and is suitable for a large-scale production. In addition, physical and chemical quality indexes of the mature vinegar provided by the present invention can reach the national quality standard of the vinegar.