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Lodderomyces elongisporus and application thereof

A technology of S. longiflorum yeast and shredded tobacco, which is applied in the field of applied microorganisms, can solve the problems that have not been found in public reports, and achieve the effects of promoting the healthy development of the cigarette industry, increasing the aroma of tobacco leaves, and improving the quality of tobacco leaves

Active Publication Date: 2014-11-26
CHINA TOBACCO YUNNAN IND
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Problems solved by technology

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  • Lodderomyces elongisporus and application thereof
  • Lodderomyces elongisporus and application thereof
  • Lodderomyces elongisporus and application thereof

Examples

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Embodiment 1

[0025] - Collection of test tobacco leaf samples

[0026] The tobacco leaf samples used in this study were all collected from the tobacco aging workshop of the raw material department of Hongyun Tobacco (Group) Co., Ltd. The specific conditions of the tobacco leaves are shown in Table 1.

[0027] Table 1. Tobacco leaf samples tested

[0028]

[0029] -The cultivation method of L. elongisporus OF-08 (L. elongisporus OF-08).

[0030] 1) Test tube slant culture

[0031] Yeast Extract Peptone Dextrose Medium (YPD) medium was used, and the formula was (gram / liter, g / v): 20 grams of peptone, 20 grams of glucose, 10 grams of yeast extract, 20 grams of agar, and distilled water to 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate L.elongisporus OF-08 and culture at 28°C for 24 hours to obtain test tube species.

[0032] 2) Preparation of bacterial agent

[0033] A. Seed culture (YPD medium), the formula is (gram / liter, g / v): 20 grams of peptone, 20 gra...

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Abstract

The invention discloses a Lodderomyces elongisporus and application thereof, belonging to the technical field of application of microbes. The classification designation of the strain is Lodderomyces elongisporus OF-08. The strain is collected at General Microorganisms Center in China Committee for Culture Collection of Microorganisms on October 8th, 2012, and the collection number is CGMCC No.6645. The physiological-biochemical characteristics of the OF-08 strain and 26S rDNA gene sequence (the sequence number in GeneBank database is JQ916053) are primary characteristic references for identifying the strain. The strain preparation can be sprayed to enhance the aroma quality of tobacco, increase the reducing sugar content and lower the contents of proteins, cellulose, total nitrogen and nicotine. Therefore, the sugar / nitrogen ratio, sugar / base ratio and nitrogen / base ratio tend to increase, so that the chemical quality is more harmonious, and the tobacco smoking quality is improved.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms, and more specifically, the invention relates to a spore-forming yeast capable of improving the quality of flue-cured tobacco. Simultaneously, the present invention also relates to the use of the said S. longitum in tobacco production. Background technique [0002] Tobacco is one of the important pillars of our country's finances. In the past two decades, tobacco taxation accounts for about 10% of the country's fiscal revenue every year, and has made great contributions to my country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overc...

Claims

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Application Information

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IPC IPC(8): C12N1/16A24B15/20C12R1/645
Inventor 段焰青冯斌杨培香者为夏建军朱保昆党立志王文元
Owner CHINA TOBACCO YUNNAN IND
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