The invention relates to the technical field of
natural product extraction and separation, and discloses a
spore oil preparation method with a high
ganoderma triterpenoid extraction rate, the method comprises pretreatment, supercritical extraction, separation treatment and purification treatment, impurities and dust are removed through screening treatment to obtain pure spores, and the spores are dried to obtain the
ganoderma triterpenoid spore oil with the high
ganoderma triterpenoid extraction rate. Wall-breaking treatment is carried out by combining a low-temperature
superfine grinding technology, so that the
spore powder achieves high
fineness and high wall-breaking rate, spore
cell walls are destroyed, and the specific surface area is increased, thereby ensuring that ganoderma triterpenes are fully dissolved out in the subsequent supercritical extraction process, and improving the extraction rate; the extraction pressure and the extraction temperature are controlled, an entrainer
ethanol is added, the dissolving property of
supercritical carbon dioxide is improved, polar components such as ganoderma triterpenes are efficiently extracted, meanwhile, through preheating treatment, temperature
impact is reduced, component degradation is avoided, and the stability and the extraction efficiency of the extraction process are guaranteed.