Animal plant and microbial cell wall lytic enzyme reactive liquid and its application

A technology for microbial cells and plant cells, applied in the field of dissolving enzyme reaction liquid compositions, can solve the problems of low activity of wall-breaking enzymes, long wall-breaking time, and incompatibility, and achieves good biodegradability, high wall-breaking rate, and ease of use. absorption effect

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned wall-breaking techniques can only break the cell wall of a single plant, and are not universal, and the plant body needs to be processed before breaking the wall, and the breaking time is long, the activity of the breaking enzyme is low, and the breaking rate is low. Low
At present, there is no enzyme reaction substance that can simultaneously break the wall of animal, plant, algae and microbial raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 1. Preparation of cell wall lytic enzyme:

[0028]Bacillus subtilis K-77 is used as the selected strain, and bee pollen is used as the medium. The medium formula: 75% pine pollen, 23% rice bran or bran, 1% sucrose, 1% gypsum powder, water-to-material ratio 1.5 : 1.

[0029] Cultivation conditions and cultivation methods: (1) Mix the medium well, and the moisture content of the compost reaches about 65%; (2) Mix the compost well and put it into a fermenter, and the temperature of the fermenter is controlled between 30°C and 37°C , adding cultivated strains, the fermentation time is 72 hours, and the pH value is 3.0-4.0; (3) ventilate, stir, and stir once every 8 hours; (4) filter after fermentation, remove slag, and take the supernatant; (5) The supernatant is precipitated by adding ammonium sulfate, and the precipitate is taken; (6) drying in an oven below 60°C to obtain coarse enzyme powder.

[0030] Get the cell wall dissolving enzyme, protoplasmic solutio...

Embodiment 2

[0042] Embodiment 2 Prepare cell wall dissolving enzyme with the method of embodiment 1, just wherein medium raw material pine pollen is replaced with Ganoderma lucidum spore powder, and get cell wall dissolving enzyme, protoplasmic solution, enzyme accelerator, inorganic salt, Mineral water and auxiliary materials are mixed to prepare the cell wall dissolving enzyme reaction liquid:

[0043] 1. Protoplasm solution 10%, is apple juice;

[0044] 2. Enzyme accelerator 10%, including Tween 80 5%, red yeast rice powder 5%;

[0045] 3. Inorganic salt 3%, where KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.2%, NH 4 NO 3 1.4%, CuSO 4 0.2%, KCl 0.5%, ZnSO 4 0.2%, FeSO 4 0.2%, MnSO 4 0.2%;

[0046] 4. Cell wall dissolving enzyme 2%;

[0047] 5. Excipients 9%, including brown sugar 3.6%, agar 2%, citric acid 3.4%;

[0048] 6. Mineral water 66%.

[0049] Use the cell wall dissolving enzyme reaction solution prepared above to break the wall of Ganoderma lucidum spore powder, put th...

Embodiment 3

[0053] Example 3 Prepare the cell wall-dissolving enzyme by the method of Example 1, but the selected strain is Bacillus subtilis YT-25, the medium raw material pine pollen is replaced by rape pollen, and the cell wall-dissolving enzyme and protoplast are obtained according to the following weight percentage and composition solution, enzyme accelerator, inorganic salt, mineral water and auxiliary materials and mixed to prepare the cell wall dissolving enzyme reaction solution:

[0054] 1. Protoplasm solution 32.6%, including soybean juice 5%, corn steep liquor 5%, pear juice 10%, apple juice 10%, egg white 2.6%;

[0055] 2. Enzyme accelerator 10%, Tween 80;

[0056] 3. Inorganic salt 1%, where KH 2 PO 4 0.2%, NaCl 0.3%, (NH 4 ) 2 SO 4 0.5%;

[0057] 4. Cell wall dissolving enzyme 2%;

[0058] 5. Excipients 10.4%, including agar 2%, citric acid 3.4%, peptone 5%;

[0059] 6. Mineral water 49%.

[0060] Use the cell wall dissolving enzyme reaction solution prepared abo...

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PUM

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Abstract

A kind of animals and plants and microbial cell wall dissolving enzymes reaction solutionú¼which belongs to enzyme dissolving technique of cell engineering. The character is: the reaction solution includes cell wall dissolving enzymes, protoplasm solution, enzymes promoter, inorganic salt, mineral water and other ingredients. The cell wall dissolving enzyme reaction solution isní»t poisonous and has good biological degradation ability. As a result of fermenting of animals and plants and microorganism, the molecular structure of the product has changed completely. The mass of biological active substance such as small cell protein, free amino acid, linolenic acid and polypeptide increase a lot. Also, these substances can be absorbed easily by human.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis in cell engineering, and in particular relates to a dissolving enzyme reaction liquid composition which has a strong effect of breaking the cell walls of animals, plants and microorganisms. Background technique [0002] Most animals and plants, especially green plants, marine animals, algae plants and microbial cells contain a variety of cell active components, and have various health care and medical functions. Such as Ganoderma lucidum spores, bee pollen, chlorella, ginseng, astragalus, periwinkle, shark chondroitin, etc. Due to the dense cell wall structure of the above substances, it is difficult to digest in the human body, making it difficult for the active ingredients to be absorbed and utilized by the body. Cell wall breaking technology must be used to effectively use the active ingredients in the cells. At present, the more advanced cell wall breaking technology is the metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12N9/36
Inventor 林陆山
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