The invention relates to the technical field of
plant polysaccharide extraction, in particular to a process for synergistically extracting
rape pollen polysaccharide by a compound
enzyme, which comprises the following steps: crushing
rape pollen to 200-300 meshes by pulse
airflow, performing
microwave-ultrasonic
degreasing by using a 85%
ethanol solution, mixing with an
acetic acid-
sodium acetate
buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound
enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by
cellulase,
pectinase,
protease and beta-
glucosidase according to a
mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG
C constant-temperature water bath and a 15V / cm pulsed
electric field, carrying out
enzyme deactivation on enzymatic
hydrolysate, carrying out gradient
ethanol precipitation, carrying out
ultrafiltration deproteinization, and carrying out vacuum pulsation
freeze drying to obtain the
polysaccharide. According to the method disclosed by the invention, the
cell wall
breakage rate and the enzymolysis efficiency are improved through pulsed
airflow crushing, magnetic nano
immobilized enzyme and pulsed
electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the
antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the
drying time is shortened, and efficient and low-consumption extraction is realized.