The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to
pepsin degradation, which comprises the following steps: S1
powder preparation: dissolving SOD
fermentation pure liquid in a
buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried
powder; S2
drying; S3 tabletting; S4,
coating liquid preparation: dissolving wheat
gliadin and
soybean protein in a
mixed solution prepared from water and
ethanol with a volume ratio of 1:1, wherein the
mass ratio of the wheat
gliadin and the
soybean protein is 0.5-1:1, adding
glycerol, stirring for 20 min by using a
magnetic stirrer, and ultrasonicating for 40 s; S5,
coating: placing the tablets in a
coating machine, setting air inlet volume, air
inlet temperature, pan rotating speed, atomization rate, fan face pressure, and
mortar flow, until the coating weight increases to 3%-10% in
mass fraction and the coating thickness is 20-50 μm, and then
drying and cooling. The prepared SOD is not easy to be photolyzed and can
resist gastric acid degradation to ensure the complete activity of the SOD.