67 results about "Ralstonia" patented technology

The invention relates to the field of microorganism application and environment protection and in particular relates to a phosphorus-solubilizing and cadmium-reducing microorganism bacterium agent anda preparation method thereof. The invention provides the microorganism bacterium agent with phosphorus-solubilizing and cadmium-reducing effects and the preparation method thereof; the microorganismbacterium agent is prepared from the following components: 15 to 30 parts of a compound bacterium agent and 70 to 85 parts of a carrier, wherein the compound bacterium agent contains 0.25 to 1.5 billion cfu / g of bacillus methylotrophicus, 0.10 to 0.50 billion cfu / g of ralstonia eutropha and 0.05 to 0.40 billion cfu / g of paecilomyces lilacinus; the carrier is powder obtained by drying and sieving turf and diatomite, wherein the turf has the advantages of improving water retention, ventilation and fertilizer retention capabilities of soil; the diatomite has the advantages of strong adsorption performance, neutral pH (Potential of Hydrogen) and no toxin, good mixing uniformity and the like. The phosphorus-solubilizing and cadmium-reducing microorganism bacterium agent provided by the invention is powdery; when being applied in a land preparation process, the phosphorus-solubilizing and cadmium-reducing microorganism bacterium agent can be used for effectively improving the fertility of the soil and increasing the content of effective phosphorus capable of being absorbed by crops in the soil; meanwhile, the mobility of cadmium ions is reduced through the metabolic activity of microorganisms and the absorption of the crops on the cadmium ions is reduced.

Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.

The invention discloses an application of a fermentation product of ralstonia eutropha in the field of animal feed. PHB (poly-Beta-hydroxybutyrate) is a biosynthetic thermoplastic high molecular material, and is used for the field of plastics processing as a completely biodegradable material before. The contents of protein, PHB, coenzyme Q10 in fermentation liquid or fermentation liquid dry powder of the ralstonia eutropha are measured firstly, a certain proportion of fermentation liquid or fermentation liquid dry powder is uniformly mixed with the animal feed as an additive after measurement and calculation, mixed feed with the PHB content of 0.5-5% is obtained, and then an animal is fed according to the conventional feed adding method to achieve the purpose of improving nutrition. Since the fermentation liquid or the fermentation liquid dry powder of the ralstonia eutropha is added to the common feed, substances such as the protein, PHB and the coenzyme Q10 contained in the fermentation liquid or the fermentation liquid dry powder can greatly promote animal growth and improve an immunologic function of the animal.

The invention relates to a compost decomposition additive. The additive comprises, by mass, 100 parts of fresh brewer's grains, 5-15 parts of bran, 2-4 parts of ammonium sulfate, 10-20 parts of urea,0.5-1 part of potassium dihydrogen phosphate, 0.1-0.5 part of NaCl and a strain seed solution with the total volume 10-20% of the mass of the fresh brewer's grains, wherein the strain seed solution isa mixture of seed solutions of aspergillus niger, penicillium, ralstonia and candida utilis in the volume ratio of (2-3):(2-3):(1-2):(1-2). According to the additive, the fresh brewer's grains serveas a culture medium for primary fermentation under the symbiotic action of various microorganisms, the number of bacteria is increased, and cellulase is also generated for further decomposing cellulose; meanwhile, the cellulose can be effectively degraded through the symbiotic action of various kinds of cellulase, and organic decomposition of feces of livestock and poultry can be better promoted.

The invention relates to microorganism plant vaccine capable of controlling continuous tomato bacterial wilt disease, and belongs to the agricultural intensive production technology. According to the microorganism plant vaccine capable of controlling the tomato bacterial wilt disease, a ralstonia solanacearum sibling bacterium pearson ralstonia eutropha (Ralstoniapickettii) NJQL-A6 is isolated from the rhizosphere of a healthy tomato, and the ralstonia eutropha has a weaking inhibited impact on a bacterial wilt pathogenic bacteria ralstonia eutropha (Ralstonia solanacearum) panel. However, the ralstonia eutropha possesses powerful colonization capability on the tomato rhizosphere and inside a plant, and can inhibit the infection of the ralstonia eutropha to a tomato plant. Experiments show that after the vaccine is irrigated to be applied to soil, the vaccine can breed rapidly so that an advantage group is formed in the soil, and the biocontrol efficiency can be reached up to more than 70% in the soil where the tomato bacterial wilt disease is severe. By inoculating the biocontrol bateria on the tomato plant with the root being infected by the bacterial wilt on the overground part, the further development of the disease can be effectively controlled.

The invention discloses a method for producing isoprene by adopting a cell fusion technology and a fusant constructed by the same. The method comprises the following steps: screening by utilizing a protoplast fusion technology, taking escherichia coli and ralstonia eutropha as parents and taking lipid as a unique carbon source and a resistance maker plasmid to obtain the reconstituted cell of high-efficiency transformed lipid; and fermenting by utilizing the fusant to produce the isoprene. The method disclosed by the invention can increase the velocity of lipid metabolism, enhance the yield of the isoprene and reduce the fermentation cost.

The invention provides a primer for detecting potato ralstonia solancearum and a PCR detection method adopted by the primer, relating to a primer and a PCR detection method adopted by the primer, and aiming at solving the problems that when the existing methods are adopted for detecting pathogenic bacteria of potato ralstonia solancearum, the detection range is narrow, the specificity is poor, the sensitivity is poor, and the detection efficiency is low. The primer has the sequence as follows: QK5-1: 5'-GCTAATACCGCATACGAC-3'; QK3-1: 5'-GAGCGTCAGTGTTATCCC-3'. The PCR detection method comprises the following steps: extracting the total genomic DNA, carrying out PCR detection, verifying the detection primer, and if an amplification band appears at the 591bp position, indicating that the sample is positive. According to the primer and the PCR detection method, the detection sensitivity can achieve 1pg of template DNA, the detection efficiency is high, the detected sample can be potato plant DNA and tuber DNA, even PCR detection can be carried out directly by taking bacterial liquid as a template.