27 results about "Ralstonia" patented technology

The invention relates to a ralstonia solanacearum bacteriophage LC10 capable of preventing and controlling tobacco bacterial wilt, the classification name of the ralstonia solanacearum bacteriophage LC10 is Rastonia phage, the preservation number of the ralstonia solanacearum bacteriophage LC10 is GDMCC No: 66716-B1, and the preservation date of the ralstonia solanacearum bacteriophage LC10 is July 21, 2025. The bacteriophage is a brachytail bacteriophage and can specifically split ralstonia solanacearum, inhibit the growth of the ralstonia solanacearum and enhance the disease inhibition capability of soil. Therefore, the bacteriophage LC10 can be used for preparing related preparations for preventing and controlling tobacco bacterial wilt.

The invention belongs to the technical field of wastewater treatment and microorganism application, and particularly relates to a complex microbial inoculant for treating phenolic substances in high-acid wastewater and application of the complex microbial inoculant. The complex microbial inoculant is prepared from lactobacillus acidophilus, Ralstonia piceatrii, bacillus modestus and bacillus mucilaginosus; wherein in the complex microbial inoculant, the weight ratio of the lactobacillus acidophilus to the Ralstonia piceatrii to the deposited halobacillus to the silt bacillus is (1-20): (1-10): (1-10): (1-10): (1-10). In the complex microbial inoculant provided by the invention, lactobacillus acidophilus, Ralstonia piceatrii, Mesohalobacillus sp. And Bacillus mucilaginosus are specifically matched and combined, and the tolerance of the microbial inoculant to an acid environment can be enhanced by utilizing the mutual synergistic effect of microbial strains; the effect of treating phenolic substances in the high-acid wastewater is obviously improved.

The present invention discloses a method for reducing and fixing Cr(VI) by key functional metal-reducing microorganisms based on a mutualistic symbiotic model: S1, preparing a mixed functional bacterial community electrolyte consisting of conductive bacteria and metal-reducing functional bacterial communities, wherein the conductive bacteria is Shewanella, and the metal-reducing functional bacterial communities include one or more of Bacillus cereus, Ralstonia pietermaritzii, and Paenibacillus alvei; S2, enriching an electroactive biofilm: pouring the prepared mixed functional bacterial community electrolyte into an electrolytic cell in a three-electrode bioelectrochemical system, and applying a positive potential of 100 to 300 mV; S3, reducing and fixing Cr(VI): after generating a mature anode biofilm with a stable current output, pouring out the electrolyte in the electrolytic cell, adding a new electrolyte containing Cr(VI), and applying a negative potential of 500 to 0 mV to the working electrode, so that the Cr(VI) in the electrolyte is reduced and fixed.

The invention relates to the technical field of natural medicinal chemistry, and discloses a compound M-1 with phytopathogen resisting activity and application thereof. The invention provides a novel compound and a fermentation liquor separated from a Ralstonia MWSTR-5 strain, and by measuring the inhibitory activity of the compound M-1 on fusarium oxysporum and alternaria solani, the compound M-1 is found to have relatively strong phytopathogen resistance activity and is a good natural bactericide.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention discloses a primer combination for detecting Ralstonia pickettii and a kit for detecting the Ralstonia pickettii, wherein the kit comprises the primer combination. The primer probe provided by the invention is targeted to a specific nucleotide sequence of the Ralstonia piceatrii instead of a horizontal conserved sequence, so that the Ralstonia piceatrii and other strains in the Ralstonia can be effectively distinguished, the technical defects that an existing detection method is insufficient in specificity and easy to generate false positive are overcome, and the accuracy of a detection result is improved. The detection limit can reach 100 CFU / mL, and compared with the prior art, the detection limit is reduced, and the detection sensitivity is improved. The kit disclosed by the invention can be applied to pollution detection of medical supplies such as pharmaceutical water, dialysis water, endoscope cleaning water, blood products, sterile normal saline and the like and rapid screening of hospital infection, is strong in specificity and high in accuracy, and has relatively high popularization and application values.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

The invention relates to the technical field of molecular diagnosis and early screening of tumors, and particularly discloses a plasma circulating microbial marker for early screening of lung cancer and application of the plasma circulating microbial marker. The marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and haemophilus parainfluenza (Haemophilus parainfluenza), and the marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and Haemophilus parainfluenza (Haemophilus parainfluenza). According to the method, nine characteristic microbial markers are detected in combination with a low Pass WGS technology, a high-precision diagnosis model is constructed through a machine learning algorithm, the established diagnosis model has excellent stability and clinical applicability, efficient recognition of early lung cancer can be achieved, and the detection cost can be greatly reduced.

The invention relates to Rosetonia for degrading pyridine, belongs to the technical field of microorganisms, and is used for solving at least one of the problems that the existing conventional microorganisms are difficult to degrade pyridine, the degradation efficiency is low and the like. The Rosetonia Q6 disclosed by the invention can realize efficient growth and degradation in an inorganic salt culture medium taking pyridine as a unique carbon source and energy source. The Ralstonia Q6 strain shows remarkable tolerance and decomposition capability on high-concentration pyridine pollutants, can effectively decompose pyridine pollutants in actual wastewater, and has a relatively good engineering application value in the field of treatment of high-concentration pyridine wastewater.

The invention provides application of rahnella maple XS-11 in the aspect of growth promotion of radix bupleuri, and belongs to the technical field of microorganisms. The invention provides rahnlaaceae XS-11, and the collection number of the rahnlaaceae XS-11 is CGMCC (China General Microbiological Culture Collection Center) No.35248. The rahnlaaceae XS-11 can be used for preparing a culture medium. In the invention, the bacterium rahnella malayi XS-11 is a growth-promoting bacterium screened from rhizosphere soil in a bupleurum chinense genuine producing area, the XS-11 strain has the capabilities of dissolving phosphorus, dissolving potassium and fixing nitrogen, can promote the growth of bupleurum chinense seedlings as a growth promoting agent, particularly has a remarkable effect of increasing the biomass of the root of a medicine part, and meanwhile, has a remarkable effect of promoting the growth of the bupleurum chinense seedlings. The growth-promoting bacterium can also significantly improve accumulation of saponin components at the root of radix bupleuri, can be used for artificial cultivation of radix bupleuri, provides a theoretical basis for increasing the yield and improving the quality of radix bupleuri, and has important guiding significance for reasonably utilizing biological agents to carry out ecological planting of medicinal plants.

The invention discloses a Ralstonton recombinant strain capable of synthesizing gamma-aminobutyric acid by using fructose and CO2 as well as a construction method and application of the Ralstonton recombinant strain. The construction method comprises the following steps: taking PHB synthesis defective Ralstonia as a chassis strain, and knocking out a gene gabT for coding aminobutyric acid aminotransferase in bacterial cells to obtain a recombinant strain GABA-0. A glutamic acid decarboxylase gene gadB from escherichia coli is introduced into the recombinant strain, 15 amino acids at the C terminal of the gene are knocked out, and the metabolic activity under the neutral condition is improved, so that the recombinant strain GABA-1 of the Ralstonia, which can utilize the same substrate as wild Ralstonia and can produce gamma-aminobutyric acid at high yield, is constructed. The recombinant Ralstonia strain constructed by the invention can be used for de novo synthesis of gamma-aminobutyric acid by taking CO2, fructose and the like as carbon sources.

The invention relates to the technical field of biocontrol bacteria, in particular to application of Gliocladium mucilaginosum RS44 and a composite flora of the Gliocladium mucilaginosum RS44 and Rahnella RS86 in prevention and treatment of corn root rot. The Gliocladium mucilaginosum RS44 provided by the invention is separated from corn roots which are not subjected to root rot in white cities of Jilin Province, has the characteristics of siderophore production and nitrogen fixation, has a better antagonistic effect on pathogenic bacteria fusarium graminearum, has an inhibition rate of 26.39%, has a good prevention and treatment effect on corn root rot, and can promote corn growth. Besides, the composite flora composed of gliocladium mucilaginosum and rahnella RS86 provided by the invention can effectively inhibit the growth of fusarium graminearum, has a good control effect on the corn root rot, reduces the disease index by 26.32%, and can promote the growth of corn.

The present disclosure relates to the technical field of biological treatment of exhaust gases, in particular to preparation of a complex microbial inoculant, an apparatus for preparing the complex microbial inoculant and application thereof. The preparation of the complex microbial inoculant is to select kitchen wastes, wood chips and EM fungus chaff as aerobic composting raw materials, and add contaminant degrading bacteria Pseudomonas mendocin NX-1, Stenotrophomonas sp. HY-2, Rhodococcus sp. YZ-1, Ralstonia sp. XZW-1, and Pseudomonas oleovorans DT4 during the aerobic composting process for joint culture until a compost pile becomes thoroughly decomposed. The complex microbial inoculant provided by the present disclosure can simultaneously degrade n-hexane, pentane, chlorobenzene and tetrahydrofuran, and has the remarkable advantages of simple preparation process, low cost, low carbon, environmental friendliness and the like. The formed microbial inoculant that is rich in efficient degrading bacteria does not need to adjust pH, has the advantages of short starting cycle, and stable and efficient exhaust gas purification, has stronger environmental adaptability, and lays a foundation for engineering application of purifying waste water and exhaust gases containing n-hexane, pentane, chlorobenzene and tetrahydrofuran by a biological method.

The invention provides a pleuromutilin derivative containing a thioether side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Pseudomonas solanacearum pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The invention relates to the technical field of saline-alkali soil improvement, in particular to a compound microorganism-loaded saline-alkali soil conditioner as well as a preparation method and application thereof. Compound microorganisms loaded on the saline-alkali soil conditioner comprise Ralstonia sp. Ralstonia Bul-1 and Bacillus sp. Bacillus Zn-B. The preparation method of the saline-alkali soil conditioner comprises the following steps: (1) pretreating straws by using a zinc chloride solution, and carrying out hydrothermal carbonization on the pretreated straws; (2) respectively preparing a Ralstonia seed solution and a bacillus seed solution, and then mixing to prepare a compound bacterium seed solution; and (3) compounding the zinc-modified hydrothermal carbon and the compound bacteria seed solution to form a composite material, and then mixing the composite material with decomposed mushroom dregs or an organic fertilizer and a calcium magnesium phosphate fertilizer for granulation to form the saline-alkali soil conditioner. During application, the composite soil conditioner is applied to saline-alkali soil, and the dosage per mu is 300-500 kg. Through the synergistic effect of biochar, organic fertilizer, microorganisms and the like, the pH and salinity of the saline-alkali soil are remarkably reduced, the content of organic matter and aggregates is increased, the soil structure is improved, plant growth is promoted, and remarkable economic, ecological and social benefits are achieved.

Provided herein are polypeptides, compositions comprising the polypeptides and methods for genome editing and gene regulation (e.g., activation and / or repression) using the polypeptides or the compositions comprising the polypeptides, such as, DNA binding domains derived from the genus of Ralstonia. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a TALE protein, such as, a Xanthomonas TALE protein. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a DNA binding protein derived from bacteria of the genus Ralstonia.

This invention discloses a strain of Acinetobacter piterum and its application in the control of bacterial wilt in tomatoes. The Acinetobacter piterum provided by this invention (… Acinetobacter pittii Acinetobacter pituitaria BB307, with accession number GDMCC NO. 67769, exhibits broad-spectrum antibacterial activity against eight sequence variants of Ralstonia solanaceae, making it suitable for controlling bacterial wilt in tomatoes. Furthermore, it possesses nitrogen-fixing, phosphorus-solubilizing, siderophore-producing, and indoleacetic acid-promoting abilities, which can promote plant growth in tomatoes. The Acinetobacter pituitaria BB307 provided by this invention demonstrates synergistic potential for biocontrol and growth promotion, offering a highly efficient microbial resource for green production of solanaceous crops and providing a more economical, efficient, and environmentally friendly solution for the prevention and control of bacterial wilt.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

The objective is to provide a wastewater purification solution for seafood processing plants that purifies wastewater, prevents sludge buildup, and prevents foul odors from being generated from the wastewater. [Solution] The solution is a mixture containing a culture medium obtained by culturing an aqueous solution of molasses with 10 species of bacteria from the genera Aspergillus, Bacillus, Lactopranthibacillus, Enterococcus, Nocardia, Pseudomonas, Ralstonia, Rhizopus, Rhodococcus, and Rhodosprillum, as well as iron fulvic acid, molasses, seaweed powder, and water.

This invention discloses a strain of Pantoea coffeiphila KG-43 and its application in the control of mulberry diseases. A strain of Pantoea coffeiphila KG-43 was obtained and deposited at the Guangdong Provincial Microbial Culture Collection Center on June 18, 2024, with accession number GDMCC NO: 64765. This Pantoea coffeiphila KG-43 exhibits excellent antagonistic activity against *Ralstonia solanaceae* and *Pantoea coffeiphila*, demonstrating good control effects against plant diseases caused by these pathogens, including *Ralstonia solanaceae* and bacterial wilt of mulberry, and is safe and effective. Therefore, Pantoea coffeiphila KG-43 or its fermentation products have promising applications and value in the control of plant diseases such as mulberry and in the preparation of formulations for controlling plant diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to expression clone of a peanut AhKCS12 gene and application of the peanut AhKCS12 gene. The invention discloses a peanut AhKCS12 gene. The peanut AhKCS12 gene is subjected to transient expression in a tobacco leaf of Bencheng and is inoculated with ralstonia solanacearum, and it is identified that the AhKCS12 gene can enhance the resistance of the tobacco of Bencheng to the ralstonia solanacearum. The invention discloses a peanut AhKCS12 gene. The peanut AhKCS12 gene is introduced into nicotiana benthamiana through a transgenic technology, and it is identified that the AhKCS12 gene can enhance the resistance of nicotiana benthamiana to ralstonia solanacearum.

The invention provides a pleuromutilin derivative containing an ester side chain or a silicon-oxygen side chain and application of the pleuromutilin derivative to prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The present application belongs to the technical field of hydrogen conversion and energy storage, and particularly relates to a method for expressing multi-subunit hydrogenase and producing formic acid by using formate dehydrogenase. The present application uses a pBBR1MCS-1 vector, adds a Lac promoter in front of each subunit sequence of hydrogenase, so that each gene is controlled by a single promoter, and adds a His tag to each gene for subsequent purification of expressed protein. After that, the constructed vector is transformed into Ralstonia eutropha H16 competent cells to obtain an engineering bacterium and express hydrogenase in the engineering bacterium. The recombinantly expressed hydrogenase is combined with formate dehydrogenase to realize self-circulation regeneration of NAD + / NADH in the reaction system without additional NADH, thereby efficiently converting H2 and CO2 into formic acid in a green and economical manner, and realizing resource utilization of green hydrogen and high-value conversion of greenhouse gas CO2.

The present invention provides a strain of Ralstonia YNK-FB0054 and its bacterial agent and application, belonging to the field of biotechnology. Ralstonia wenshanensis The bacterial strain provided by the present invention has the functions of zinc solubilization, nitrogen fixation, organic and inorganic phosphorus solubilization, siderophore production, and indoleacetic acid production. The bacterial strain and the bacterial agent containing it as an active ingredient can promote plant seed germination and plant growth, and have high application value.

The invention discloses an original bacteriophage phi GPN1, which is classified and named as Ralstania phage, is preserved in Guangdong Microbial Culture Collection Center on November 13, 2025, and has a preservation number of GDMCC NO: 67287-B1. The invention discloses an application of the original bacteriophage phi GPN1 in prevention and control of soil-borne bacterial wilt. The invention discloses an original bacteriophage preparation for preventing and controlling soil-borne bacterial wilt. The original bacteriophage preparation contains the original bacteriophage phi GPN1. The original bacteriophage phi GPN1 can infect a plurality of strains of ralstonia solanacearum obtained by separation in different regions, and has a wider host spectrum compared with a lytic bacteriophage.

The invention discloses a tomato bacterial wilt prevention and control method, which belongs to the technical field of tomato disease prevention and control, and comprises the following steps: S1, preparing a complex microbial inoculant: in the complex microbial inoculant, the volume ratio of bacillus velezensis bacterial liquid to stenotrophomonas rhizophila bacterial liquid to streptomyces griseorubens bacterial liquid is 1: (3-6): (2-4); the effective viable count of the bacillus riissii bacterial liquid, the stenotrophomonas rhizophila bacterial liquid and the streptomyces griseorubens bacterial liquid is not less than 1 * 10 < 8 > CFU / ml; s2, applying the complex microbial inoculant obtained in the step S1 to rhizosphere of tomato plants. The method provided by the invention can be used for effectively preventing and treating the tomato bacterial wilt caused by the Ralstonia solanacearum (Ralstonia solanacearum).

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the genuine nature of polygonatum sibiricum and application, the microbial marker combination is composed of Escherichia. Shigilla, Acremonium, Pseudomonas, Serratia, Mortierella, Wallemia, Engydonium, Sporidesmium, Ceratobasidium, Clonostachys and Ralstachys, an evaluation method for the genuine nature of polygonatum sibiricum is established, the sensitivity is 91.7%, the specificity is 100.0%, the AUC is 93.8%, and the method has the advantages of being high in sensitivity, strong in specificity and high in accuracy and can be used for evaluating the genuine nature of polygonatum sibiricum. The method can be used for judging whether the production area of the polygonatum sibiricum is a genuine Suhua production area or not, and can be widely applied to genuine evaluation of the polygonatum sibiricum.