67 results about "Septoria lycopersici" patented technology

The invention relates to microorganism plant vaccine capable of controlling continuous tomato bacterial wilt disease, and belongs to the agricultural intensive production technology. According to the microorganism plant vaccine capable of controlling the tomato bacterial wilt disease, a ralstonia solanacearum sibling bacterium pearson ralstonia eutropha (Ralstoniapickettii) NJQL-A6 is isolated from the rhizosphere of a healthy tomato, and the ralstonia eutropha has a weaking inhibited impact on a bacterial wilt pathogenic bacteria ralstonia eutropha (Ralstonia solanacearum) panel. However, the ralstonia eutropha possesses powerful colonization capability on the tomato rhizosphere and inside a plant, and can inhibit the infection of the ralstonia eutropha to a tomato plant. Experiments show that after the vaccine is irrigated to be applied to soil, the vaccine can breed rapidly so that an advantage group is formed in the soil, and the biocontrol efficiency can be reached up to more than 70% in the soil where the tomato bacterial wilt disease is severe. By inoculating the biocontrol bateria on the tomato plant with the root being infected by the bacterial wilt on the overground part, the further development of the disease can be effectively controlled.

The invention discloses separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide, and belongs to the technical field of biology. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi rhizophagus irregularis which is called arbuscular mycorrhizal fungi rhizophagus irregularis CD-1 and is separated from soils; a specific sequence of verifying ribosome 28S rDNA is shown in SEQ ID NO.1; by utilizing the fungicide prepared by the fungi, the height of strains, the number of petioles, the overground fresh weight, the overground dry weight, the root fresh weight and the root dry weight of tomatoes can be increased, and the mycorrhizal infection rate delta gt can be obviously prompted; 80 percent of corns are high in selfing line strains and heavy in overground fresh weight and overground dry weight; the resistance to banded sclerotial blight by the corns is improved, and the arbuscular mycorrhizal fungi has an extremely-high genetic use value.

The invention discloses a Brevibacillus laterosporus strain N106-2 of which the collection number is CGMCC No.8482. The strain has strong indoor flat inhibiting actions on multiple plant pathogenic fungi (fussarium wilt of tomato, root rot of chili, gray mold of tomato, Rhizoctonia solani, black spot of cabbage, anthracnose of pear and black rot of pear) and plant pathogenic bacteria (bacterial wilt of tomato, fire blight of pear and streak of rice); and the strain can secrete proteinase, cellulase and surfactant antimicrobial related substances with hemolytic activity. The Brevibacillus laterosporus can be prepared into a biocontrol aqua; the biocontrol aqua has certain preventing and treating effects on fussarium wilt of tomato, fusarium rot of chili, anthracnose of pome and eyespot of pome. The Brevibacillus laterosporus N106-2 has the advantages of no toxicity, no pathogenicity and high safety for human and livestock, is environment-friendly, is a useful resource for development of microbial pesticides, and has potential application prospects.

The invention discloses a strain of bacillus altitudinis and application thereof in control of tomato root-knot nematode. The bacillus altitudinis strain YT2002 has been deposited in the China GeneralMicrobiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15213; experimental results show that the bacillus altitudinis strain YT2002 biological agenthas a good effect on control of tomato root-knot nematode, with the control effect of 63.03% after 14 days after the last application; from growth of the tomato, the bacillus altitudinis strain YT2002 has an obvious effect on promoting tomato growth, and is safe and reliable without risk of damage.

The invention relates to radiation-resistant fruit tea vinegar containing watermelons. The radiation-resistant fruit tea vinegar containing watermelons is prepared from the following raw materials in parts by weight: 300-320 parts of watermelons, 100-120 parts of cherries, 400-430 parts of sticky rice vinegar, 40-45 parts of leaf mustard, 20-23 parts of mung bean sprouts, 35-36 parts of tomatoes, 0.5-1 part of coix seed oil, 40-45 parts of carrots, 36-37 parts of smoked plums, 8-10 parts of black tea, 50-60 parts of apple vinegar, 40-50 parts of hawthorn juice, 5-6 parts of pseudo-ginseng and the like. The radiation-resistant fruit tea vinegar containing watermelons disclosed by the invention is prepared from the cherries, the sticky rice vinegar and the like; by means of the added fresh watermelon juice, the radiation-resistant fruit tea vinegar containing watermelons is full-bodied in fragrance and has a certain effects of clearing heat and resisting radiation; and in addition, by means of the added extracts, such as pseudo-ginseng, cinnamon, lucid ganoderma, antrodia and rhodiola rosea, the radiation-resistant fruit tea vinegar containing watermelons disclosed by the invention has a certain effects of resisting radiation, invigorating circulation of blood, eliminating stagnant blood and the like.

The invention relates to a culture method and use of Penicillium Chrysogenun Snef 1216 inducing meloidogyne incognita chitwood resistance in tomatoes after treating tomato seeds and belongs to the technical field of microbial pesticides. The fungus strain Snef 1216 the invention relates to was collected in China General Microbiological Culture Collection Center on December 14, 2010. The collection number of the strain is CGMCCNO.4469. The strain is prepared by liquid fermentation culture. After being treated by the metabolite of the strain, the tomato seeds can generate meloidogyne incognita chitwood resistance during germination. The tomatoes treated by the metabolite of the fungus can effectively control the generation and spread of a disease caused by meloidogyne incognita chitwood, and have a bright application and development prospect.

The present invention provides a strain of Ochrobactrum pseudogrignonense NC1 and applications of the biocontrol microbial agent of Ochrobactrum pseudogrignonense NC1. According to the present invention, the results of the biological test of Ochrobactrum pseudogrignonense NC1 fermentation broth on the egg and the second instar larvae of Melanogyne incognita show that the strain can inhibit the Melanogyne incognita egg hatching and the second instar larvae activity through the volatile nematode killing active substance in the fermentation broth; after the biocontrol microbial agent prepared by using the Ochrobactrum pseudogrignonense NC1 as the active component is applied on potted tomatoes, the test results show that the strain can significantly increase the tomato above-ground part fresh weight, achieves the Melanogyne incognita prevention effect of 62.5%, and has the effect equivalent to the nematode killing agent 10% fosthiazate; and the Ochrobactrum pseudogrignonense NC1 and the biocontrol microbial agent thereof provide good application prospects in prevention and control of root-knot nematode in vegetables.

The invention discloses a multifunctional insecticide fertilizer for tomatoes and a preparation method of the multifunctional insecticide fertilizer. The multifunctional insecticide fertilizer is prepared from the following raw materials in parts by weight: 31-31.62 parts of furfural residue, 13-13.4 parts of urea, 21-21.6 parts of potassium sulfate, 23-23.5 parts of monoammonium phosphate, 1.8-2 parts of zinc sulfate, 1.8-2 parts of polyvinyl alcohol, 1.8-2 parts of polyacrylamide, 2.1-3.2 parts of bactericide, 0.2-0.4 part of citric acid, 0.06-0.08 part of compound sodium nitrophenolate and 0.16-0.2 part of EDTA. The multifunctional insecticide fertilizer for tomatoes disclosed by the invention is reasonably and especially designed according to the plentiful-lack indexes of nutrients of the soil in Hexi corridor in Gansu Province, and the absorption ratio of the tomatoes on nutrient elements; a series of problems that a greenhouse tomato field in Hexi corridor in Gansu Province is unbalanced in ratio of macroelements to microelements, low in organic matter content, and poor in water retaining capacity, and generates soil hardening, alternaria solani, late blight, gray mold, viral disease, bacterial wilt, septoria lycopersici and the like are solved, and the multifunctional insecticide fertilizer integrates nutrition, disease prevention, soil improvement and water retention.

The invention discloses bacillus safensis for producing protease and resisting botrytis cinerea. The bacterial strain is classified and named as Bacillus safensis SH180 and is preserved in CGMCC (China General Microbiological Culture Collection Center) on April 8, 2018; the preservation number is CGMCC15565. The bacterial strain has the effects of promoting the biological growth, producing the protease and resisting the botrytis cinerea. The bacterial strain is applied to production; important significance is realized for improving the tomato yield, reducing the harm of pesticide to irrigationwater bodies and reducing the generation of drug-resistance bacteria.

The invention discloses application of extract of celery stems and leaves. The extract of celery stems and leaves is applied as an inhibitor for diseases of tomato. It is found in the invention that the extract of celery stems and leaves can effectively inhibit the growth rate of Pseudomonas solanacearum and root rot fungus attacking tomatoes and substantially alleviates the harm of Pseudomonas solanacearum and root rot fungus to tomato plants. The extract of celery stems and leaves is convenient to use, low in cost, purely natural and free of the problems of residues and resistance.

The invention discloses a preventing and treating method of the tomato yellow leaf curl virus. Tomatoes are cultured in a solar greenhouse according to size, wherein the large line spacing is 80 cm, and the small line spacing is 60 cm; gathering inducing plants are transplanted 15-20 days before tomatoes are planted, wherein a line of gathering inducing plants are planted between two lines of planted tomatoes, and the row spacing is 25 cm; substrate seedling trays containing carbonized rice husks are adopted for culturing seedlings of the gathering inducing plants, carbonized rice husks are additionally applied in planting pits, and seedling tray dipping and root irrigating are conducted through liquid thiamethoxam; before the gathering inducing plants are transplanted, a vent hole of thesolar greenhouse is closed by a 40-mesh sophocarpidine pest-killing protection net. On the premise of greatly reducing the chemical pesticide consumption and achieving main crop non-contact chemical prevention and treatment, the morbidity of the tomato yellow leaf curl virus can be effectively reduced, and the morbidity of the tomato yellow leaf curl virus of the infected tomato species can generally be controlled to be 4% or lower.

The invention discloses a method for synchronously detecting four whitefly transmitted gemini-viruses infecting tomatoes. The method can be used for simultaneously identifying four viruses by utilizing four specific primers ToL-F/ToL-R, TY-F/TY-R, PaL-F/PaL-R, AY-F/AY-R especially for four viruses, namely, tomato leaf curl China virus, tomato yellow curlleaf China virus, papaya leaf curl China virus and ageratum yellow vein China virus by combining a PCR (polymerase chain reaction) detection technology. According to the method, after a total DNA (deoxyribonucleic acid) of a sample is extracted, the four whitefly transmitted gemini-viruses can be detected by only once PCR detection, and a simple method is provided for investigation and identification. Compared with the traditional method which can be used for detecting one virus by once PCR, the method has the advantages of reducing cost, saving time, guaranteeing sensitivity and specificity and simplifying operation, and is a specific, sensitive, convenient, economical and effective detection method.

The invention discloses an environment-friendly pesticide for preventing and treating tomato trialeurodes vaporariorum. The environment-friendly pesticide contains a tomato seed soaking agent and a tomato plant spraying agent, wherein the tomato seed soaking agent contains the following raw materials: folium isatidis, tobacco leaves, artemisia apiacea, fresh ginger, mint, radix stemonae, rhizoma arisaematis, fructus quisqualis, rheum officinale, Sichuan pepper, realgar and calomel; and the tomato plant spraying agent contains the following raw materials: rhizoma cyrtomii, radix stemonae, rhizoma arisaematis, betel nut, cinnabar, realgar, fructus quisqualis, rhizoma pinelliae, artemisia apiacea, herb of tuberculate speranskia, folium isatidis, a dispersing agent, a defoaming agent and a suspending aid. By utilizing a pure traditional Chinese medicine preparation, chemical pesticides are not added, so that the environment-friendly pesticide has a good pest killing effect, has no residue of toxic medicines, can be absorbed by plants and is a nutrition solution with a pest killing function.

The invention discloses a preparation method of a pesticide for preventing and treating vegetable fungal disease microorganisms, belonging to the technical field of microorganisms. The method comprises the following steps of: activating and culturing strains of streptomyces diastatochromogenes D which is separated and sifted from a soil sample collected from Tianmu Mountain in Linan, Hangzhou, Zhejiang in China; fermenting and culturing; extracting leavening; and mixing agricultural pesticide auxiliary materials with a purified active compound to prepare the agricultural pesticide in a plurality of formulations. The microorganism agricultural pesticide can be used for preventing and treating vegetable fungal diseases, such as cucumber fusarium wilt, tomato gray mold or tomato early blight, and the like, and is safe; and the preventing and treating effect of the agricultural pesticide are equivalent to or slightly better than that of the traditional chemical agents.

The invention provides a method for identifying silverleaf whitefly resistance of tomato germplasm. The method comprises the following steps: selecting second and/or third fully-expanded leaves below growing points of known silverleaf whitefly-resistant germplasm, known silverleaf whitefly-infected germplasm and to-be-identified germplasm at the same seedling age as in vitro egg-carrying materials; inoculating adult silverleaf whitefly to an insect cage; standing for 4 hours to 3 days; and determining resistance of germplasm to silverleaf whitefly according to the quantity of silverleaf whitefly eggs on the back of the leaves. The method can effectively evaluate the level of silverleaf whitefly resistance of different tomato materials. Compared with insect resistance identification using living leaves of tomato plants, the method is more accurate, time-saving and labor-saving, and is more suitable for mass screening of tomato germplasm materials with silverleaf whitefly resistance.

The invention discloses an atractylodes macrocephala special microbial agent and a preparation method thereof. The atractylodes macrocephala special microbial agent is prepared by fermenting at leasttwo of bacillus subtilis, bacillus amyloliquefaciens and bacillus methylotrophicus. Through synergism effects of the three plant probiotics of bacillus subtilis, bacillus amyloliquefaciens and bacillus methylotrophicus, the atractylodes macrocephala special microbial agent reduces squaring blooming of atracylodes macrocephala, promotes root and stem growth of atracylodes macrocephala, increases the yield of atracylodes macrocephala, and in field experiments, increase the yield per mu (=0.0667 hectare) of atracylodes macrocephala by 220 kilograms. Meanwhile, the atractylodes macrocephala special microbial agent improves resistance of atractylodes macrocephala to diseases such as ceitocybe bescens, leaf blight, southern blight and septoria lycopersici, reduces or even avoids application of pesticides, reduces pesticide residues of atractylodes macrocephala and improves the quality of atractylodes macrocephala. In field experiments without application of any pesticides, plants can be robust in growth and free from diseases and achieve a survival rate of 100%.

The invention discloses lactobacillus fermentium and an application of lactobacillus fermentium for preventing and treating a plant fungal disease and belongs to the field of biocontrol agents. The bacterial strain is obtained by separating from a soil planted with tomatoes and the classification name is lactobacillus fermentium; the Latin name of the strain is Lactobacillus fermentium, the reference microorganism is NJHYHWGY20130877, the preservative date is 17th, January, 2014, and the accession number in the preservative center is CGMCC No.8735. The lactobacillus fermentium is fermented and cultured to obtain fermentation liquor, the fermentation liquor is condensed to prepare the lactein concentrated liquor, and the concentrated liquor is diluted and sprayed on the crop leaf surface; and the bacterial strain has good effect for inhibiting the crop fungal disease, simultaneously has the functions of promoting the crop growth and meets ecological agriculture requirement.

The invention belongs to the field of agriculture and provides an application method for applying vanilline under soil conditions to prevent and treat meloidogyne incognita chitwood. The application method is used for applying the vanilline to soil. Particularly, at the seedling age of tomatoes with three leaves and one heart to tomatoes with five leaves and one heart, seedlings are transplanted, one seedling is planted in each pot, and the vanilline is applied to the soil after seedling transplantation, wherein an application mode is a direct application mode or a drop irrigation mode and by dry weight of the soil, the application amount of the vanilline is 0.2 to 8.0mmol/kg dry soil. According to the invention, the prevention and treatment effects of the vanilline on meloidogyne are observed and an experiment of applying the vanilline to the soil by adopting a direct application method and simulating a drop irrigation method so as to prevent and treat the meloidogyne incognita chitwood is performed. When the vanilline with concentration of 0.2 to 4.0mmol/kg dry soil is directly applied to the soil under the soil conditions, not only are normal growth and development of tomatoes ensured, but also infection and reproduction of the meloidogyne are inhibited, wherein the tomato root-knot number inhibition ratio reaches 26.5 percent to 57.2 percent and the egg mass inhibition ratio reaches 29.5 percent to 46.2 percent.

The present invention provides an essential oil containing cineole for eliciting tomato resistance to disease by fungal infection. The present invention also provides a method to elicit tomato resistance to disease by fungal infection.

The invention relates to application of peach-auxin-primary-response-factor Ppa011935m gene to tomatoes. The Ppa011935m gene is obtained after cloning from peach fruits, and is transferred into the tomatoes and applied, and the result shows that the plant type of the tomatoes can be changed through Ppa011935m gene overexpression, the internode of the tomatoes extends, the plant height of the tomatoes is increased, the fruit shape of the tomatoes is changed, acuminate fruits are formed, the quantity of seeds in fruits is decreased, and even seedless fruits are formed. Meanwhile, Ppa011935m transgene promotes releasing of fruit ethylene, and the fruits are matured in advance. The invention provides a method capable of changing the plant type of the tomatoes, the seed quantity and promoting fruit maturation, and the method includes the step that the Ppa011935m gene is introduced into the tomatoes, and correlated characters of nutrition growth and reproductive growth of the tomatoes can beeffectively changed.

The invention discloses an artificial celery seedling culture method. The method comprises the steps of celery seed preparation, celery seed soaking pretreatment, celery seed pharmaceutical treatment, celery seed sterilization, celery seed germination acceleration and celery seed sowing. Compared with existing celery seedling culture techniques, the method has the advantages that the germination rate of seeds is 95.5-98%, seedling survival rate after transplantation is 91-94%, the cultured seedlings are high in growth vigor and have a strong root system and high disease resistance, the occurrence rate of leaf spot disease, soft rot and septoria lycopersici is reduced remarkably in the culture period, adaptability is high after transplantation, the high and low temperature resistance of celery is improved obviously, the width and number of celery stems are increased remarkably, yield is increased notably, and economic benefits are high.

Relating to the field of disease-resistant cultivation techniques of vegetables and plant metabolite extraction, the invention discloses a method for collection and extraction of disease-resistant rootstock root exudate. The method includes: seedling raising of test solanum lycopersicum rootstock variety, hydroponic collection, extraction and concentration, thus obtaining the plant root exudate. The test solanum lycopersicum rootstock variety solanum lycopersicum rootstock No.1 and/or solanum lycopersicum rootstock No.21. The method provided by the invention is simple in operation, safe and environment-friendly, does not destroy the plant itself, and the obtained root exudate has high purity, and can inhibit the bioactivity of soil ralstonia solanacearum when applied in practical production, thus alleviating the problem of high incidence of ralstonia solanacearum in solanum lycopersicum producing areas.