Method for identifying silverleaf whitefly resistance of tomato germplasm

Abstract

The invention provides a method for identifying silverleaf whitefly resistance of tomato germplasm. The method comprises the following steps: selecting second and / or third fully-expanded leaves below growing points of known silverleaf whitefly-resistant germplasm, known silverleaf whitefly-infected germplasm and to-be-identified germplasm at the same seedling age as in vitro egg-carrying materials; inoculating adult silverleaf whitefly to an insect cage; standing for 4 hours to 3 days; and determining resistance of germplasm to silverleaf whitefly according to the quantity of silverleaf whitefly eggs on the back of the leaves. The method can effectively evaluate the level of silverleaf whitefly resistance of different tomato materials. Compared with insect resistance identification using living leaves of tomato plants, the method is more accurate, time-saving and labor-saving, and is more suitable for mass screening of tomato germplasm materials with silverleaf whitefly resistance.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to a plant insect resistance identification method, in particular to an identification method of tomato germplasm resistance to whitefly. Background technique [0002] Bemisia tabaci originated in tropical and subtropical regions and has spread all over the world (Barro et al., 1998). It is one of the most serious insect pests in field and horticultural crop production in the world (Gerling and Mayer, 1996). Bemisia tabaci is an omnivorous insect that feeds on more than 900 species of plants (Brown et al., 1995). [0003] Tomato is an important vegetable crop in the world. Common tomato cultivars have previously been reported to have low resistance to B. tabaci (Freitas et al., 2002). Bemisia tabaci can directly feed on the juice of tomato phloem, resulting in poor plant growth and development; secrete honeydew on tomato fruits and leaves, leading to abnormal ripening and mildew of t...

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Problems solved by technology

But this method also has some disadvantages. The bemisia tabaci used for infestation must be of the same age and a certain ratio of males and females. The early collection of bemisia tabaci is cumbersome and complicated; the space of the micro insect cage is small, which affects the free movement of bemisia tabaci ; Some tomato leaflets are small and cannot bear the weight of the micro-insect cage, and whiteflies are easy to escape; the area is relatively large and the workload is heavy, so it is not suitable for the identification of insect resistance in groups

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