11results about How to "Does not affect growth" patented technology

The present application relates to the field of bioengineering, and particularly relates to a protein mutant capable of improving the nucleoside yield of a strain and application thereof. The present application performs point mutation on adenine deaminase and pyrimidine nucleotide transport protein, which can effectively accumulate the inosine, adenosine and other nucleoside related products produced by the strain, does not affect the growth of the bacterial body, has wider practicality, and is conducive to reducing the cost of industrial production of nucleosides.

The invention discloses an intelligent constant-temperature culture system for algae. The system comprises an intelligent control unit, a constant-temperature circulating water unit and an algae culture unit, the intelligent control unit is used for intelligently regulating and controlling the temperature of the constant-temperature circulating water unit, so that the temperature of water entering the algae culture unit is constant, and meanwhile, various water quality parameter probes are arranged in the algae culture unit and are used for transmitting real-time detection parameters in the algae culture stage back to the intelligent control unit for data storage and real-time display. The device is reasonable in structural design and excellent in function, and does not cause large cold and hot impact on a culture sample in a constant temperature process to affect the growth of the culture sample. In addition, parameters such as air supply time and flow can be intelligently adjusted and set in combination with the real-time growth parameter state of the algae, it is ensured that the optimal air supply effect is achieved, and the cellular morphological structure is not affected or growth is inhibited while the algae culture growth rate is increased.

This invention belongs to the field of microbial technology and provides a microorganism coated with a metal-polyphenol secondary coordination nanocoating, its preparation method, and its applications. This invention involves the self-assembly of polyvinylpyrrolidone, metal ions, and polyphenols to form metal-polyphenol nanoparticles. Subsequently, a continuous nanocoating is rapidly deposited on the surface of the microorganism using the secondary coordination between the nanoparticles and the metal ions, resulting in a coating thickness of approximately 100 nm. The coating construction method of this invention is applicable to different types of microorganisms, and the coating has no significant impact on the normal growth of the microorganisms, exhibiting good biocompatibility. Simultaneously, this nanocoating can selectively promote the biofilm formation of Gram-negative probiotics (EcN). This promoting effect is maintained in a simulated intestinal mucus system. This invention can be used to improve the adhesion and colonization ability of probiotics and is applicable to the construction of probiotic preparations and microecological regulation systems.

The invention relates to the field of PHA production, and discloses a method for increasing the yield of PHA, which is characterized by comprising the following steps: inoculating a PHA fermentation strain into a fermentation culture medium for fermentation under the condition that PHA can be produced by fermentation, and supplementing sulfur-containing acid radical ions in the fermentation process. By adopting the method, the number of thalli can be increased, higher PHA content can be obtained, and the growth of strains is not influenced.

The invention discloses a preparation method and application of a perilla leaf traditional Chinese medicine residue-based charcoal composite material. Belongs to the technical field of soil remediation materials. The method comprises the following three steps: by taking perilla leaf extraction residue as a raw material, performing pretreatment, performing gradient oxygen-limited cracking and preparing a finished product: firstly, air-drying the raw material until the water content is less than or equal to 15%, crushing and sieving with a 2mm sieve; nitrogen is introduced at the speed of 40-60 mL / min to serve as shielding gas, the temperature is increased to 180-220 DEG C at the speed of 8-12 DEG C / min to be cracked for 1-2 h, the temperature is continuously increased to 400-450 DEG C to be cracked for 3-6 h, and the temperature difference of two sections of cracking is larger than or equal And finally, quenching with nitrogen, crushing and sieving with a 0.15 mm sieve to obtain a finished product. The adsorption performance of the prepared biochar composite material on Pb < 2 + > and Cd < 2 + > is remarkably improved; and the preparation process is free of chemical addition, low in raw material cost and suitable for in-situ remediation of Pb < 2 + > and Cd < 2 + > contaminated soil, and resource utilization of the medical solid waste is achieved.

The invention relates to the technical field of fermented milk products, in particular to fermented milk containing bifidobacteria with high viable count and a preparation method of the fermented milk. Specifically, when the fermented milk containing the probiotics is prepared, raw milk, white granulated sugar, wheat hydrolyzed protein powder, inulin and milk protein powder are jointly used as raw materials of the fermented milk, and the viable count of the probiotics bifidobacterium in the fermented milk is increased by using the wheat hydrolyzed protein powder, the inulin and the milk protein powder according to the mass ratio of (1-4): (1-10): (1-8). By adding the prebiotics, the milk protein powder and the plant protein powder composition into the formula, the bifidobacterium fermented milk has a remarkable value-increasing effect, and the problem that the viable count of the bifidobacterium-containing probiotic fermented milk is low is effectively solved.

The utility model discloses a sludge wet oxidation waste gas soil filter treatment device which comprises a spray absorption unit, a biological soil filter unit and a waste gas collection and exhaust unit, the spraying absorption unit performs reverse heat and mass transfer on waste gas to be treated and an intermediate medium in a spraying tower, the waste gas subjected to heat exchange enters the biological soil filter tank unit, is subjected to microbial decomposition through a filter tank filler layer and an active soil layer, is collected by a transparent collection cover of the waste gas collection and exhaust unit, and is subjected to high-altitude up-to-standard emission. The waste gas treatment device is low in waste gas treatment cost, simple, stable and reliable; the transparent collecting cover is made of a light-transmitting material, so that photosynthesis of herbaceous plants can be maintained, and strain loss caused by rain wash can be prevented; and the waste gas collection and exhaust unit is used for organized emission, so that enrichment of organic matters in a factory environment is avoided. The device combines spraying and biological soil filter unit treatment processes, achieves up-to-standard emission of waste gas, and guarantees long-term stable operation of the system.

The utility model relates to the technical field of rose planting, and discloses an adjustable rose planting spraying device which comprises a supporting mechanism and an adjusting mechanism, the adjusting mechanism is movably connected to the top of the supporting mechanism, the adjusting mechanism comprises a reciprocating unit and a spraying unit, the reciprocating unit is movably connected to the top of the supporting mechanism, and the spraying unit is movably connected to the top of the supporting mechanism. And the spraying unit is movably connected to the surface of the reciprocating unit. According to the adjustable rose planting spraying device, the adjustable and reciprocating spraying head is adopted, the irrigation water amount and the spraying range can be adjusted according to actual requirements, waste of water resources is avoided, meanwhile, negative effects on the environment are reduced through precise irrigation, the development concepts of green, low carbon and environmental protection are met, and the adjustable rose planting spraying device is worthy of popularization and application. When used in an installable mode, the device can be suitable for various scales of rose planting sites, can be customized and transformed according to actual needs, is small in installation occupied space, and does not affect the growth of roses during spraying.

The present application relates to a strain of Bacteroides fragilis and its application, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 14, 2026, and the preservation number is: CGMCC No. 38257.The strain can regulate STING-dependent training immunity, effectively resist Salmonella typhimurium and H9N2 avian influenza virus infection, maintain the integrity of the intestinal barrier of the host, and reduce inflammatory damage.The Bacteroides fragilis of the present application has good biological safety, and the strain can be used as a training immunity inducer, and the strain is expected to be a candidate probiotic or vaccine adjuvant for preventing / treating bacterial and viral infections, suitable for human health and poultry farming, and provides a new solution to resist antibiotic resistance.

The present invention relates to a maltogenic amylase mutant with improved enzyme activity and a screening method thereof, belonging to the field of bioengineering technology. The present invention first constructs a maltogenic amylase mutant library, then uses P in a recombinant Bacillus subtilis glvc and P amyE The promoters express fluorescent protein and maltogenic amylase mutants, respectively. glvc After mutation, the response range and induction intensity to maltose were further improved. Since the maltogenic amylase mutant reacts with soluble starch to produce maltose, under the induction of maltose, P glvc The promoter expresses fluorescent protein, so the fluorescence intensity difference of the recombinant strains is used to screen the highly active maltogenic amylase mutant AmyM. Q440N and AmyM S442N / Q661L The mutant has been significantly improved in enzyme activity, catalytic efficiency and thermal stability compared with the wild type, and can be more widely used in hydrolyzing starch to prepare maltose.

The application belongs to the technical field of genetic engineering, and particularly relates to a CtPYL6 protein related to plant drought resistance, a gene and application thereof. The application provides a CtPYL6 protein related to plant drought resistance, wherein the amino acid sequence of the CtPYL6 protein is shown as SEQ ID NO. 1. The CtPYL6 protein provided by the application can improve the drought resistance of plants. The results of examples show that overexpression of the gene can improve the seed germination rate, the chlorophyll content of plants and the photosynthetic efficiency of plants. CtPYL6 Compared with wild type plants, overexpression of the gene can reduce the accumulation of malondialdehyde, superoxide anion and hydrogen peroxide, and significantly improve the activities of superoxide dismutase, catalase and peroxidase, thereby improving the drought resistance of plants. CtPYL6 The CtPYL6 protein provided by the application can obtain new germplasm with the drought resistance characteristics, and is of great significance for carrying out drought-resistant crop breeding.