807results about How to "Does not affect growth" patented technology

The present invention provides a comprehensive herbicide for paddy fields, which includes component A and component B, wherein component A is mesotrione, and one or more of component A and component B Item compound, used for weeding in the field; component B is alachlor, metolachlor, propachlor, acetochlor, pretilachlor, butachlor, mefenacet, quinclorac , Cyhalofop-methyl, pendimethalin, saponin, bispyribac, saflufenacil, cyclopyrafen, pyrifluben. After the above scheme is adopted in the present invention, the composition of component A and component B has a synergistic effect, and has a good control effect on both grass weeds in paddy fields and broad-leaved and sedge weeds , and the residual effect period is short, does not affect the growth of the next crop, and is friendly to the environment.

The invention discloses a method for producing CIS-4-hydroxyproline. The method comprises the steps of obtaining a recombinant strain by constructing an overexpression prolyl hydroxylase gene Ecp4H in a host bacteria cell and inhibiting expressions of a prolyl hydroxylase gene putA during degradation pathway of proline and succinate dehydrogenase genes sucA and sucB, an isocitrate lyase gene aceA and an isocitrate kinase gene aceK during degradation pathway of alpha-ketoglutaric acid by utilizing a CRISPR-Cas9 technology, and conducting whole-cell transformation and production on the CIS -4- hydroxyproline after conducting fermentation and cultivation on the recombinant strain. The method for the CIS -4- hydroxyproline is simple in cultivation mode and high in strain vigor and product yield.

The invention provides a tree pool. The tree pool comprises a front pool (11), a filtration pool (3) and a split-flow pipe. A water inlet (8) is formed in the front pool (11), and a division plate (7) is arranged in the front pool (11). The front pool (11) is divided into a split-flow chamber (12) and a spilling chamber (20) by the division plate (7), and the split-flow chamber (12) and the spilling chamber (20) are communicated. The split-flow pipe is provided with an inlet and an outlet, the inlet of the split-flow pipe is disposed in the split-flow chamber (12), the outlet of the split-flow pipe is disposed on the outside of the tree pool, and the inlet of the split-flow pipe is higher than the bottom of the front pool (11). Besides, the invention further provides a method for filtering runoff rainwater through the tree pool. According to the tree pool and the method, sewage interception and emission reduction can be performed on road initial runoff rainwater, rainwater is used for greening irrigation and preventing plants from being damaged by snow-melting agents.

The invention belongs to the field of disinfectants, and concretely relates to a Chinese herbal medicinal disinfectant for the breeding industry. The disinfectant is based on a Chinese herbal medicinal extract liquid, the Chinese herbal medicinal extract liquid comprises, by mass, 25-35% of a garlic extract liquid, 15-25% of a Herba Houttuyniae extract liquid, 15-20% of a purslane extract, 9-15% of an Artemisia argyi, gingko and Artemisia carvifoliaextract liquid, and 10-20% of a pine branch and leaf extract liquid, and the mass percentage content sum of all the above extract liquids is 100%. The disinfectant can control fungal and bacterial diseases, can improve the disease resistance of bred animals (poultry fish and the like), prevents the propagation of diseases, ensures the normal life order of the bred animals without transition, can be directly used to disinfect breeding places (ponds, farmlands and birdhouses) and transport machines and apparatuses, and can kill pathogens on objects in contact with the animals in the animal breeding environment, and the effective rate of the disinfectant is above 85%.

The invention discloses a germinated whole grain and a preparation method thereof, and belongs to the technical field of deep processing of grains. The whole grain is subjected to ultrasonic washing, high-voltage pulsed electric field treatment, biological enzymolysis or combined high-voltage pulsed electric field and biological enzymolysis treatment in a soaking process, and a segmented soaking and germination manner for germination is adopted, so that the sensory quality, germination yield and quality of a germinated whole grain product are improved, and germination loss is reduced. The preparation method is simple, low in soaking and germination temperature, short in cycle, energy-saving and environment-friendly. The content of functional, nutritional and bioactive substances in the germinated whole grain product is remarkably increased, and food safety is remarkably improved. For example, for brown rice, germinated brown rice prepared by the preparation method has gamma-aminobutyric acid content of 303.8 to 312.6 mg / 100 g, glutathione content of 16.8 to 18.3 mg / 100 g, IP6 content of 460.8 to 484.4 mg / 100 g and dietary fiber content of 3.5 to 3.6 mg / 100 g.

The invention belongs to the field of new energy utilization, and particularly relates to a heat pump type greenhouse air conditioning system with a solar photovoltaic power supply function and an underground water source. The heat pump type greenhouse air conditioning system with the solar photovoltaic power supply function and the underground water source comprises an underground water source and heat pump system and a solar photovoltaic power generation system, a greenhouse is refrigerated and heated by the underground water source and a heat pumps, and power is supplied for the underground water source and heat pump system by the aid of a solar photovoltaic technology. The underground water source and heat pump system comprises an underground water heat exchange system, a heat pump unit and a tail-end system, the solar photovoltaic power generation system comprises a solar photovoltaic array, a direct-current power distribution cabinet, an alternating-current power distribution cabinet and a grid-connected inverter, the solar photovoltaic power generation system and a public power grid are matched with each other to supply the power, effects of continuous energy conservation and emission reduction can be realized, and shortcomings of instability and high cost of solar energy are overcome. Besides, amorphous thin-film solar panels are adopted, are low in manufacturing cost and high in light transmittance, can generate the power without affecting growth of crops, and do not additionally occupy land when built on a roof of the greenhouse, sunshade for the greenhouse in summer is omitted, and the investment is saved.

The invention provides a root thorn-proof and waterproof coiled material, which comprises a modified asphalt waterproof layer, a metal foil layer and a polyester tire, which are composited together. The polyester tire is presoaked by special modified asphalt. The root thorn-proof and waterproof coiled material is formed by the composite of the modified asphalt waterproof layer, the metal foil layer and the polyester tire, wherein the polyester tire is presoaked by the special modified asphalt, so that the root thorn-proof and waterproof coiled material has double functions of the water resistance and the penetration resistance of plant roots, can prevent from being penetrated by plant root hairs, and keeps the waterproof function for a long time.

The invention relates to a new use of polyurethane hard bubble, which in detail is used as filling materials for the rejuvenating and curing of told and good trees in the tree holes. The application of the said polyurethane hard bubble in the tree rejuvenating and curing is realized by the following steps: connecting the concrete iron and iron wire to indefinite shape and putting it into the tree hole as inner support; mixing and stirring the liquid of blowing agent- isocyanate and polyether with the proportion to be 1:0.5-1.5; pouring the mixture mentioned above into the tree hole with 70-300 kg per cubic meter; fixing and sealing with preformed mold in the out, pressing bark line in the top or spiking one layer of real bark. The invention is characterized by the following aspects: water resistance, antiseptic, compression, wind resistant, resistant to flame, long working life, near contraction and expansion with the tree, elastic, the effective prevention of the continuing tree rotting and damage by the compact association with the tree, little influence of the tree growth, beautiful and simple shaping process.

The invention discloses a low fish meal mixed feed for pelteobagrus fulvidraco. The mixed feed is prepared from the following raw materials by weight percent: 15-20 percent of soybean meal, 8-10 percent of corn protein powder,10-16 percent of soybean protein peptide,15-20 percent of fish meal, 25-28 percent of wheat flour, 6-8 percent of rapeseed meal, 2-2.5 percent of calcium biphosphate, 2-3 percent of zeolite powder,4-5 percent of soybean oil, 0.5-2 percent of fish oil, 0.5 percent of vitamin premixing materials and 0.5 percent of mineral premixing materials. The mixed feed is prepared from the following steps: weighing the components according to the weight proportions; adding the materials in a mixing machine to mix fully; puffing and pelletizing; packaging to obtain a finished product. According to the mixed feed, the dependence of the pelteobagrus fulvidraco on the fish meal is reduced; the palatability of the mixed feed and the growing effect of the pelteobagrus fulvidraco are not affected; the performance-price ratio is high; the problems of limited fish meal resources, high price and low performance-price ratio are solved; the mixed feed had a wide market application prospect.

The invention relates to a traditional Chinese medicine preparation for preventing and curing paralichthys olivaceus and sea cucumber scuticoci liatlda diseases. The preparation comprises the following traditional Chinese medicine components in percentage by weight: 5-20 percent of abrotanum, 5-20 percent of azedarach, 5-20 percent of cyrtomium fortunei, 5-40 percent of stemona, 5-20 percent of golden cypress, 5-20 percent of betel nut and 5-30 percent of omphalia, and the sum of the weight percentages are 100. According to the invention, medicines with components restraining or killing pathogens are selected to make a formula on the basis of traditional Chinese theories, breeding bait is added and absorbed by the host of a parasite, scuticoci liatldas parasitizing in the body or on the body surface of the host of the parasite are completely killed in a body fluid absorbing and supplying way, so that the traditional Chinese medicine preparation has the advantages that medicine feeding is simple, the medicine effect is quick, the insert killing effect is good, the recovery of the physique of the host of the parasite is quick, the survival rate is high, side effects and medicine residue are avoided, as a result, economical loss caused by scuticoci liatlda infection during the production processes of breeding and growing.

The invention relates to a seedling scion grafting method, which comprises the following steps of (1) sun-shading greenhouse, cutting seedling bed and cutting substrate preparation; (2) scion and stock preparation: new tips of a well-bred parent tree under the healthy growth condition are selected as scions, new tips of a well-bred parent tree under the strong growth condition are selected as stocks, the length of the stocks is about 2 to 10cm, 1 to 2 sections of leaves are left, and the left leaves are positioned in positions being 1 to 2cm below an upper shearing opening and positions being 1 to 4cm above a lower shearing opening; (3) stock pretreatment; (4) grafting: a cleft grafting method is adopted, the stock is flatly placed to be tightly adhered to a base plate, and a split opening with the length being 0.5 to 1cm is longitudinally cut in the position of the upper shearing opening, the scions with the length being 2 to 3cm is sheared, 1 to 2 buds are remained, a two-sided wedge shape with the sharp top end and the length being 0.8 to 1.2cm is cut in a position being 1cm below a scion leaf, the cut scions are aligned with the split opening of the stock, in addition, at least one side is required to form layer aligning, the cut scions are slightly inserted so that the cut scions and the stock are tightly pasted, the middle position of an aluminum sheet covers a grafting opening, and the aluminum sheet is tightly pinched and folded back so that the fixation is realized; (5) cottage; and (6) daily management.

The invention relates to a spraying device, and in particular relates to a spraying device of chemical fertilizer for gardens. Aiming at solving the technical problems, the invention provides the spraying device of the chemical fertilizer for the gardens, which can be used for spraying the chemical fertilizer after soil is loosened, saves time and labor and is used for uniformly spraying so that the growth of the plants is not influenced. In order to solve the technical problems, the spraying device of the chemical fertilizer for the gardens, provided by the invention, comprises a trolley, a box body, a liquid charging pipe, a liquid feeding pipe, a first check valve, a vertical rod, a first rotary table and the like; the box body is mounted at the left side of the top of the trolley in a bolt connection manner; the liquid charging pipe is connected with the left side of the top of the box body; the liquid charging pipe is communicated with the inner part of the box body; the lower part of the right side of the box body is connected with the liquid feeding pipe; the liquid feeding pipe is communicated with the inner part of the box body; the first check valve is arranged on the liquid feeding pipe. The spraying device of the chemical fertilizer for the gardens has the effects that the chemical fertilizer can be sprayed after the soil is loosened; the time and labor are saved and the chemical fertilizer is uniformly sprayed, so that the growth effect of the plants is not influenced.

The invention discloses a garden agricultural spraying vehicle with a rapid mixing device. The garden agricultural spraying vehicle comprises a bottom plate. An electric push rod is fixedly connectedwith the upper surface of the bottom plate. The top end of the electric push rod is fixedly connected with the outer surface of a water pipe. The left end of the water pipe is communicated with the right side face of a spraying nozzle. The right end of the water pipe is communicated with a water outlet of a first water pump through a first hose. The garden agricultural spraying vehicle with the rapid mixing device is provided with a first tank body, a second tank body, a second water pump, a second motor, a rotating shaft, a bearing, a first motor, a gear, a rack rod and a control panel, so that when pesticides or fertilizer needs to be sprayed on crops, different types of pesticides or fertilizer is poured into the first tank body and the second tank body respectively, the second motor iscontrolled to make a stirring blade to stir the different types of pesticides or fertilizer in a shell, a farmer does not need to stir the pesticides or fertilizer first and then carry the stirred pesticides or fertilizer onto the spraying vehicle, and the time and labor of the farmer are saved.

The invention relates to a dust suppressant for suppressing mineral powder and dust exposed on the ground and a preparation method thereof. The dust suppressant comprises dust suppressing powder and water according to a mass ratio of 1:(30-150), the dust suppressing powder comprises, by weight, 5-45 parts of gelatinizing agent, 15-50 parts of auxiliary material and food-grade preservative accounting for 2-18% of total mass of the dust suppressing powder, the gelatinizing agent comprises one or multiple of sesbania gum, carboxymethylcellulose, polyacrylamide, latex powder, maltodextrin, guar gum and xanthan gum, and the auxiliary material comprises tapioca gelatinized starch, sodium humate, glucose, humic acid, attapulgite and diatomite. Through reasonable matching with modified starch, high bonding force with the mineral powder and good wind-erosion-resistant dust suppressing effect, a prepared dust suppressant solution is low in viscosity, little in power needed for spraying, high instability, less prone to layering and low in using cost, and production raw materials of the dust suppressant itself are easy to get and low in cost.

The invention discloses a TiO2-loaded packing. The packing comprises polyurethane sponge blocks and TiO2, wherein TiO2 is loaded on the polyurethane sponge blocks. The invention further discloses a preparation method of the packing. The method comprises steps of: soaking the polyurethane sponge blocks into a TiO2-rich solution after being concentrated and treated in oil bath; heating the polyurethane sponge blocks in a drying oven until a suspension liquid is evaporated out and TiO2 is completely loaded on the polyurethane sponge blocks; washing completely with deionized water; and drying to obtain the TiO2-loaded packing. The cost of raw materials of TiO2 is low in cost, and a TiO2 film loaded on the external surface of the polyurethane sponge block is thin and uniform and does not affect the growth of the microorganism in a porous structure in the packing. Experiments prove that the TiO2-loaded packing has high degradation removal rate to active black 5. Photocatalytic degradation and biodegradation are coupled together, so that the packing integrates the advantages of advanced oxidation and biodegradation, and has a good treatment effect on complicated degradation-resistant organic matters, particularly on printing and dyeing wastewater.

The invention relates to a method for jointly repairing submerged plants on a river underwater hard slope, which comprises the following steps of: coating a planting matrix and inserting the root systems of submerged plants or spreading the submerged plants by using spinning geotextile as a bottom layer and a hempthread net as a top layer to manufacture a geomembrane blanket, and reserving rivet holes and plastic bolt holes for fixation at four corners, each edge and the center of the geomembrane blanket; fixing the upper end of the geomembrane blanket at the upper part of the underwater slope surface at the normal water level of a river by using bolts when the geomembrane blanket is used; and spreading the geomembrane blanket to the downward slope surface, driving bolts into the reserved rivet holes for fixation, and dumping stone at feet of the slope to press the edges. According to the area range of the river underwater hard slope to be repaired, the specification of the geomembrane blanket and the quantity of the combination geomembrane blankets can be freely selected to form the repairing region of submerged plants on the joint type river underwater hard slope. The method provided by the invention has the effects of purifying river water quality, conserving moisture, and the like, and the purposes of repairing the hard protecting slope of the river bed, purifying water quality and improving the ecological environment of the river are achieved.

The invention relates to a cultivation device, a multilayer three-dimensional cultivation system comprising the cultivation device, and a plant factory cultivation system comprising the multilayer three-dimensional cultivation system. The cultivation device comprises a planting box and a planting plate which can be moved integrally, wherein a nutrient solution inlet and a nutrient solution outletare positioned on the same side of the planting box; the multilayer three-dimensional cultivation system comprises a support shelf and a nutrient solution supply system arranged on the support shelf;and the cultivation device can be integrally and detachably arranged on the support shelf. The planting box and the planting plate of the cultivation device provided by the invention can be taken andput together, so that plant seedlings are protected from being damaged, a phenomenon of nutrient solution leak dripping is avoided, the independent taking and placing of each cultivation device does not affect the growth of other plants on the support shelf, and the production management is simplified. The multilayer three-dimensional cultivation system provided by the invention solves the difficult problems of liquid supply, automatic operation, cultivation device and pipeline cleaning, environmental control and the like in three-dimensional cultivation of plants.

The invention discloses constructed recombinant escherichia coli and a method for biosynthesis of 3'-sialyllactose. The recombinant escherichia coli is named as E.coli-XYY which has a synthesis pathway of the 3'-sialyllactose. Meanwhile, the invention also discloses a constructing method. At the same time, the invention solves the problems of the prior art and provides an efficient gene knockout scheme; an original strain, after undergoing genetic engineering transformation, can produce the 3'-sialyllactose only, without changing other properties of the strain, so that influence on fermentation production is avoided; since mature escherichia coli plasmid is adopted by the strain, bacterium growth and normal metabolism do not affected in a metabolic process. The recombinant escherichia coli constructed by the invention has a good application prospect; therefore, a new thought is provided for the production of the 3'-sialyllactose by virtue of a biological method.

The invention relates to a vehicle storing and shifting apparatus which comprising a transfer platform, a transfer main rod, a footing foundation, a power source and a power-driven transfer device, wherein one end of the transfer main rod connects to a movable coupling device, which is mounted on the footing foundation, the other end of the transfer main rod connects to the transfer platform by movable connection through the connector, one end of the power-driven transfer device is fixed, the other is connected with a power-assisted support bar, automatic wheel fasteners are arranged on the transfer platform, the power source is connected with the power-driven transfer device. The device can transfer a car to the roadside embellishment belt from the roads.

The invention relates to the technical field of plant cultivation, in particular to a cultivation method for interplanting soybeans in newly planted sugarcane fields. The cultivation method particularly includes simultaneously cultivating sugarcanes and the soybeans in the newly planted sugarcane fields in February every year; interplanting two rows of soybeans between each two adjacent rows of sugarcanes during cultivation, and applying solid water to the sugarcanes and the soybeans after seeds are sown. The solid water is formed after drought-resistant and water-absorbent polymers absorb moisture with the volume 200-300 times the volume of the drought-resistant and water-absorbent polymers. The cultivation method has the advantages that owing to the solid water capable of continuously releasing the moisture for up to 1-2 months, the sugarcanes and the soybeans can be simultaneously planted early, prompt soybean harvesting is unaffected, a high germination rate and a high seedling rate of sugarcane seedlings can be guaranteed, and the purpose of double good yields of the sugarcanes and the soybeans in the newly planted sugarcane fields can be achieved; by the aid of the cultivation method, the land utilization rate can be effectively increased, the crop yield can be increased, and the economic benefit can be improved.

The invention discloses a device for bagging fruit paper bag and is characterized in that the device is composed of seven parts of a bag containing casket (1), a bag feeder (2), a mouth splaying component (3), a mouth pressing component (4), a mouth closing and sealing component (5), a handle (6), and a bracket (7). The device is small in volume, simple in structure, convenient in operation, low in cost, is lightweight and portable, durable, and reliable, is fast and smooth in the whole bagging process; a bagging operation can be finished in about 2-3s, and the bagged bag has tightly sealed mouth, is weather-proof, has swollen bag body, so that the bag can not contact with the fruit, thus not influencing growth of the fruit and greatly improving speed and effect of bagging the fruit.

The invention provides a method for using bionic aquatic plants to cover the suspended submarine pipeline. First, sand and gravel are used to backfill the scoured groove at the bottom of the submarine pipeline, and after filling, a bionic anti-scour protection system is installed on the backfill layer; Flexible installation and construction, in line with environmental protection requirements.

The invention provides a fluorescence labeling method for sea cucumber larvas. Sea cucumber is one of rare marine products, and wild sea cucumber resources are increasingly deficient. Artificial propagation and releasing is an important method for restoring the wild sea cucumber resources. In order to assess propagation and releasing effects, released individuals need to be regularly captured back, so that how to effectively distinguish artificially propagated individuals from wild individuals and establish a labeling method becomes an urgent need in the industry. A lot of sclerites are distributed at the body wall of the sea cucumber, and a main ingredient of the sclerite is calcium carbonate. Aiming at the characteristic, the invention provides a fluorescence labeling method for sea cucumber larvas, which comprises the following steps: soaking the sea cucumber larvas for 20-24 hours in a dark environment by using 200 mg / L calcein, or injecting the 200 mg / L calcein into sea cucumber coelomic cavities for labeling through an injection way. After labeling, more than 50% of the sclerites in the body wall are infected with the calcein, and fluorescence detection rate of the sclerites labeling the individuals is still above 5% after breeding for a year. The fluorescence labeling method can not affect the ingestion and growth of the sea cucumber larvas, and the marker calcein can be thoroughly removed out of the body through metabolism of the sea cucumber two years later.

The invention relates to a recombinant HBV (Hepatitis B Virus) vector for expressing specific HBVshRNA (Hepatitis B Virus Short Hairpin Ribonucleic Acid) as well as a construction method and application of the vector. The recombinant HBV vector is formed by deleting 2111st-2643rd bases, 187th-680th bases or 1060th-1500th bases from an LJ196 vector, and replacing shRNA transcription structure units of the deleted sequences at the deleted sequence deleting positions. The preparation method is simple; RCDNA (Relaxed Circular Deoxyribonucleic Acid) can be efficiently synthesized using Core protein and Pol protein of wild-type hepatitis B virus genome by adopting the recombinant HBV vector, and the synthesis efficiency is higher than that of the wild-type hepatitis B virus genome DNA, the RCDNA synthesis can compete with the duplication of the wild-type hepatitis B virus, simultaneously, the shRNA can be efficiently expressed by utilizing the characteristics of high synthesis efficiency of the recombinant HBV vector, and then the DNA duplication and HBsAg synthesis of the hepatitis B virus can be disturbed. The vector can be applied to preparation a medicine for disturbing the DNA duplication of the hepatitis B virus genome, and a new idea for treating the chronic hepatitis B virus infection is provided.

The invention discloses a composite purification regulating and controlling system for a water body in a cultivation pond, which is vertical to pond banks at two sides of the cultivation pond. A plurality of micro hole aerator pipes are uniformly configured at the bottom of the bond; a strand upper rope cord is placed correspondingly at the water level position above each micro hole aerator pipe; aquatic plants are sown on the strand upper rope cords at intervals; meanwhile, floating balls are installed on the rope cords for maintaining buoyancy; a plurality of vertical artificial water plants are distributed uniformly between each aerator pipe and the corresponding strand upper rope cord; and upper ends of the artificial water plants are fixed on the upper rope cord, and the lower ends of the artificial water plants are fixed on the aerator pipes which are fixed by fixing blocks. The system combines micro hole oxygen increasing, biochemical treatment and biological purification organically, improves the oxygen dissolving efficiency and biological purification effect, and has the characteristics that structure is compact, regulating and controlling are carried out in situ, energy consumption is low, installation is simple, and the like.

The invention relates to a seed paper tape sowing method. A seed paper tape is prepared by the steps of sending a paper tape into a paper tape conveying device by a paper tape feeding device, enabling the paper tape to move from an inlet to an outlet at a constant speed along with a conveyor belt of the conveying device, sequentially fixing a dispensing device, a seed spreading device and a spread seed blowing device above the conveyor belt, fixing a spread seed collecting device, corresponding to the spread seed blowing device, below the conveyor belt, and fixing a seed paper tape finished product packaging device at the outlet end of the conveyor belt. The seed paper tape sowing method comprises the steps of 1, firstly, leveling soil; 2, removing a plastic package from a seed paper tape finished product and tiling the seed paper tape; 3, covering with soil, namely after the seed paper tape is sowed, uniformly covering the tiled seed paper tape with soil; and 4, watering and irrigating. The seed paper tape sowing method provided by the invention has the advantages of solving the problem of separation of high-quality seeds with good methods, being beneficial to the integration of propagating, breeding and promoting of high-quality seeds, facilitating the simplified sowing, saving high-quality seeds and labor force, and enabling the cost to be reduced and the benefit to be increased.

The invention relates to the technical fields of traffic engineering and civil engineering, and discloses a public traffic system and a construction method thereof. The public traffic system comprises at least one of the following systems of a lane traffic system (2) and an overhead track traffic system (3). The public traffic system is characterized in that the lane traffic system (2) or the overhead track traffic system (3) comprises an intersection node commercial traffic hub transfer building (4); crossings are intersection nodes of lanes (21); the lanes (21) comprise at least one type of motor vehicle lanes (21.1), non-motor vehicle lanes (21.2), ground walkways (21.3), overpasses (22) and tunnels (23); and the intersection node commercial traffic hub transfer building (4) is arranged in at least one of the following lane (21) crossing positions of crossing centers, crossing corners, interiors and exteriors of crossing edges, parts above the crossings, and parts below the crossings. The public traffic system needs a small investment or does not need a government investment, allows low operation and maintenance costs, has a short construction period, and can fully develop subway functions of a high speed and a large passenger transport amount. Therefore, the public traffic system perfectly solves public traffic problems.

The invention relates to the field of microorganisms and discloses a method for high-density fermentation of pichia pastoris of insulin precurosor protein. The method comprises the steps of expanding culture of strain, fermentation culture and thallus collection. Fermentation culture is divided into a batch growth phase, a glycerinum replenishment phase and a methyl alcohol induction phase. By controlling parameters including age, replenishing rate and pH value of strain during fermentation, the concentration of glycosylation 2 can be effectively reduced by 60+ / -8%, and the unit yield of insulin precurosor protein can be increased by 25% or above. Furthermore, the method has the advantages of being simple, easy to operate, low in cost and the like.

The invention belongs to the technical field of planting lycium ruthenicum murray, and provides a method for cultivating and planting the lycium ruthenicum murray in order to solve the problems that an existing lycium ruthenicum murray planting method is greatly limited and is difficult to form an industrial planting technology. The method is characterized by carrying out container cultivation at first, and then transplanting bare-rooted seedlings in a field. According to the method provided by the invention, cultivation management on the lycium ruthenicum murray is completed by adopting the combination of the container cultivation and field cultivation, and container seedlings are convenient to manage; for example, the distance between nursery stocks can be regulated at any time according to the growth situation of the nursery stocks; reshaping and trimming are convenient, and the like; transportation is convenient, and time and cost for seedling lifting and packaging of the field cultivation can be saved; the nursery stocks can be transplanted all the year round, the quality and the growth of the nursery stocks cannot be affected, the original tree form can be kept, and a greenery landscape effect is increased; long-term sustainable planning management can be achieved by field cultivation management, the cost is reduced, the resource is saved, industrial planting of planting cultivation, comprising later-period management, of the lycium ruthenicum murray can be achieved, and meanwhile, the growth situation of the nursery stocks of the lycium ruthenicum murray can be conveniently fed back by farmers in time.