67 results about "Culture growth" patented technology

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

The invention relates to a preparation method of a biomass charcoal based acid soil regulator, and belongs to the technical field of a soil regulator. The preparation method disclosed by the invention comprises the steps of: firstly, carrying out fermentation by utilizing livestock manure, wood flour, soil rhizobium and the like to obtain a soil nutrient medium; then carrying out ball milling on dolomite, coal ash and the like to prepare composite stone powder; then mixing the composite stone powder with the soil nutrient medium, and transplanting pea plants to carry out culture growth; activating nutritional ingredients in soil by utilizing the growing process of the pea plants; then carrying out pyrolysis on the pea plants to prepare biomass charcoal; after activating the biomass charcoal, mixing the biomass charcoal with culture soil powder, and transferring a mixture into a granulator to prepare particles; after carrying out packaging and bagging, obtaining the biomass charcoal based acid soil regulator. The soil regulator prepared by the preparation method disclosed by the invention can break soil hardening, loosen the soil and improve gas permeability of the soil, has functions of improving the soil, storing water for drought resistance, reinforcing disease resistance of crops, improving yield of the crops, improving quality of agricultural products and recovering the original ecology of the crops, and greatly improves a planting survival rate and yield of the agricultural products.

The invention provides a grifola frondosa three-stage culture production method. The grifola frondosa three-stage culture production method comprises the steps of first-stage culture production, second-stage culture production, third-stage culture production, culture package production, inoculation and cultivation, bud forcing and mushroom cultivation. The third-stage culture production comprises the steps of obtaining of culture medium recipes, production of the culture media, bagging, sterilization, inoculation, cultivation of the culture and raising of the culture. The grifola frondosa three-stage culture production method is characterized in that the culture medium recipes include the wood strip culture medium recipe and the wheat grain culture medium recipe, and the production of the culture media comprises production of a wood strip culture medium and production of a wheat grain culture medium. Compared with a wood chip culture, the culture obtained through the grifola frondosa three-stage culture production method has the advantages that inoculation efficiency is 5-8 percent higher, the maximum culture growth distance is 50-100 percent shorter, the cultivation efficiency is 20-60 percent higher, each culture bag is consistent in mycelial age vertically, so that nutrients are released in a centralized mode, and the first-batch mushroom yield can be increased by 15-30 percent. Compared with liquid culture, the culture obtained through the grifola frondosa three-stage culture production method is applicable to existing culture production and detection, inoculation equipment and technicians in China, and can reduce the cultivation infection rate by 2-5 percent.

The invention provides high-efficiency sulfate reducing bacteria (the bacteria is named Desulfotomaculum halophilum RETECH-SRB-II, the preservation unit is China Center for Type Culture Collection in Wuhan University, the preservation date is May 11th, 2007, and the preservation registration number is CCTCC NO of M207061) with wider growth temperature and pH applicability; the invention simultaneously provides a technique that can treat acid mine wastewater with the carbon and nitrogen source of straws. In the treating of the acid mine wastewater, the technique reaches the Cu<2+> removing rate of 89 to 97 percent, the Zn<2+> removing rate of 90 to 98 percent, the Fe<2+> removing rate of 91 to 99 percent, the Fe<3+> removing rate of 79 to 91 percent, and the As<3+> removing rate of 80 to 90 percent. The treated wastewater takes neutral pH value. The technique has the advantages that straws can be used as the carbon and nitrogen source required for culture growth, thus lowering the bacteria cultivation cost by 30 to 50 percent.

The invention provides a fungus strain A.flavus G10 for degrading polyurethane plastic. The microbial preservation number of the fungus strain A.flavus G10 is GDMCC 60537. The invention also providesa method for culturing the fungus strain A.flavus G10. The method comprises the following steps that the fungus strain is separated from the intestinal tract of a cricket; PU serves as a unique carbonsource, the fungus strain is cultured in a liquid culture medium to obtain a culture solution; the culture solution is diluted and coated on a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato dextrose agar culture medium to obtain a culture growth substance; and the culture growth substance is subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A.flavus G10 is high in speed of degrading the polyurethane plastic.

The invention relates to the technical field of culture media of desert alga, and provides a culture medium capable of promoting the growth of desert alga and a method for culturing the growth of thedesert alga by adopting the culture medium. In the culture media, soluble salt ions comprise Na+, K+ and Mg<2+>, and the concentration of the soluble salt ions is 10<-6>mM to 10<-2>mM. The method forculturing the growth of the desert alga comprises the following steps: inoculating activated desert alga cells to the culture medium of the desert alga, and carrying out static culture on the culturemedium under the light condition, wherein during static culture, a small amount of the culture solution is taken from the culture medium each day, when the number of cells of the desert alga or the optical density value at 540nm of the culture solution is measured to be stable, the culturing of the desert alga is completed. According to the technical scheme, the culture medium capable of promotingthe growth of the desert alga is obtained through allocation, so that an excellent method for culturing the growth of the desert alga is obtained, the number of the cells of the desert alga is improved by 30%-50% compared with the prior art, the yield of the desert alga is effectively improved, and the large-scale culture of the desert alga is facilitated.

The invention discloses culture solution and a culture growth method of silver gray pearls. The culture growth method is characterized by comprising the following steps of: utilizing raw material ingredients of 1 to 5 parts of dry fish scale, 2 to 5 parts of ginkgo skin, 2 to 6 parts of actinolite, 2 to 6 parts of dry achyranthes bidentata, 1 to 6 parts of parasitism leaves, 1 to 5 parts of adenosine disodium triphosphate and the like and respectively preparing aqueous solutions of the raw material ingredients; firstly, soaking nuclei in mixed solution of the fish scale solution, the ginkgo skin solution, the actinolite solution and the achyranthes bidentata solution in a warm environment for 3 to 7h, taking out the nuclei and air-drying for 3 to 5h; then soaking the nuclei in the parasitism leaf solution for 3 to 5h, taking out the nuclei and air-drying for 2 to 3h; finally, after soaking the nuclei into 10 to 20ml of dilute solution of the adenosine disodium triphosphate, immediately taking out the nuclei, completing transplanting cultured objects into pearl shells in 1 to 3min and placing the pearl shells in which transplanting of the cultured objects is completed into a sea area to culture for 1 to 3 years so as to grow into the pearls. According to each obtained pearl, the surface is silver gray and is smooth; a pearl layer and the nucleus are tightly combined; pearl brightness is strong and soft; the color is bright; and the pearl is bright, smooth, gorgeous and noble.