A strain of sulfate reduction bacteria and process for using the same for treating acidic mine wastewater with straw as carbon nitrogen source

A sulfate and process technology, applied in the field of microorganisms, can solve the problems of unfavorable industrial production promotion and application, high price, and high consumption cost of pharmaceuticals, and achieve the effects of reducing bacterial culture cost, wide pH adaptability, and wide growth temperature.

Inactive Publication Date: 2009-05-20
有研资源环境技术研究院(北京)有限公司
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AI Technical Summary

Benefits of technology

This patented technology allows creating high efficiency Sulfuric Acid Reduction Bacterium that has better temperatures and p H values than previous methods like Desulfothermus thermosensitive bacteriocine or Microbacteux niger. It also uses rice stalk instead of expensive materials used during production. Overall this results in lower costs compared to current technologies while still maintaining effective activity against harmful substances such as copper ions from mineral acids containing these metals.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to grow sulfur oxidizing bacillus (Sox) without consuming excessive amounts of resources like fossils fuel oil, ammonium salts, etc., while also effectively reducing harmful levels such as arsenite and selenium into harmless water during mineral extractions processings.

Method used

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  • A strain of sulfate reduction bacteria and process for using the same for treating acidic mine wastewater with straw as carbon nitrogen source
  • A strain of sulfate reduction bacteria and process for using the same for treating acidic mine wastewater with straw as carbon nitrogen source

Examples

Experimental program
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Effect test

Embodiment 1

[0016] First, the sulfate-reducing bacteria strain preserved at low temperature (the bacterial name is Desulfotomaculum halophilum RETECH-SRB-II, preservation unit: China National Center for Typical Culture Collection, address Wuhan University, preservation date: May 11, 2007, preservation registration No.: CCTCC NO: M207061) with 10-20ml medium (K 2 HPO 4 , 0.1-3g; (NH 4 ) 2 SO 4 , 0.5-5g; NaHCO 3 , 0.1-3g; CaCl 2 , 0.1-1g; MgSO 4 , 0.2-2.5g; sodium lactate, 0.5-5g; trace element, 10-15ml; trace vitamin, 10-15ml; cysteine ​​hydrochloride, 0.1-2g; yeast extract, 0.5-5g; (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O, 0.1-2g; distilled water, 1L) in a test tube for activation. The activated strains are transferred into 100-200ml of culture solution, and after 18-36 hours of cultivation, the culture is expanded to 300-500ml with 5-20% (v / v) inoculum.

[0017] Cut the fresh straw into small pieces of 1-3cm, pile them into piles, soak the pile with water, cover it with plastic film, ke...

Embodiment 2

[0020]First, the sulfate-reducing bacteria strain preserved at low temperature (the bacterial name is Desulfotomaculum halophilum RETECH-SRB-II, preservation unit: China National Center for Typical Culture Collection, address Wuhan University, preservation date: May 11, 2007, preservation registration No.: CCTCC NO: M207061) with 10-20ml medium (K 2 HPO 4 , 0.1-3g; (NH 4 ) 2 SO 4 , 0.5-5g; NaHCO 3 , 0.1-3g; CaCl 2 , 0.1-1g; MgSO 4 , 0.2-2.5g; sodium lactate, 0.5-5g; trace element, 10-15ml; trace vitamin, 10-15ml; cysteine ​​hydrochloride, 0.1-2g; yeast extract, 0.5-5g; (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O, 0.1-2g; distilled water, 1L) in a test tube for activation. The activated strains are transferred into 100-200ml of culture solution, and after culturing for 18-36 hours, expand the culture to 300-500ml with 5-20% inoculum size as seed solution.

[0021] Cut the fresh straw into small pieces of 1-3cm and pile them into piles, soak the pile with water, cover it with plas...

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Abstract

The invention provides high-efficiency sulfate reducing bacteria (the bacteria is named Desulfotomaculum halophilum RETECH-SRB-II, the preservation unit is China Center for Type Culture Collection in Wuhan University, the preservation date is May 11th, 2007, and the preservation registration number is CCTCC NO of M207061) with wider growth temperature and pH applicability; the invention simultaneously provides a technique that can treat acid mine wastewater with the carbon and nitrogen source of straws. In the treating of the acid mine wastewater, the technique reaches the Cu<2+> removing rate of 89 to 97 percent, the Zn<2+> removing rate of 90 to 98 percent, the Fe<2+> removing rate of 91 to 99 percent, the Fe<3+> removing rate of 79 to 91 percent, and the As<3+> removing rate of 80 to 90 percent. The treated wastewater takes neutral pH value. The technique has the advantages that straws can be used as the carbon and nitrogen source required for culture growth, thus lowering the bacteria cultivation cost by 30 to 50 percent.

Description

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Claims

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Application Information

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Owner 有研资源环境技术研究院(北京)有限公司
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