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Stappia strain and its application in degradation of zearalenone

A technology of zearalenone and Staples genus, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of single type of degrading strains, differences in degrading ability of strains, production limitations of key enzymes, etc. Achieve the effect of wide application value, wide growth temperature and easy cultivation

Active Publication Date: 2019-10-18
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a certain number of zearalenone toxin-degrading bacteria have been found at home and abroad, the types of degrading strains isolated are relatively single, and there are still certain differences in the degradation ability of each strain. The degradation mechanism is unclear, and the degradation of the secretion of degrading bacteria There are few types of enzymes, and there are still many limitations in the application of key enzymes to production

Method used

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  • Stappia strain and its application in degradation of zearalenone
  • Stappia strain and its application in degradation of zearalenone
  • Stappia strain and its application in degradation of zearalenone

Examples

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Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment one: Staple's genus ( Stappia sp. ) Isolation, screening and identification of YM29

[0029] (1) Separation and screening:

[0030] The soil samples of plant rhizosphere in Wulabo region of Xinjiang were collected, and microorganisms were isolated and purified by gradient dilution method. Weigh 10g of the soil sample, put it into 100ml of sterile water, add sterilized glass beads, shake at 150rpm, 30°C for 20min, to fully disperse the sample. Aseptically draw supernatant 100μl into 900μl sterile water, dilute with sterile water to make 10 -2 -10 -5 concentrated diluent. Take 100 μl of the dilutions of each concentration, use the conventional coating method in the inorganic salt medium containing 10 μg / mL zearalenone, place it in a constant temperature incubator at 30 ° C for 72 hours, and wait until the colonies grow on the plate. , Pick a single colony on the plate for purification and culture until there are no other colonies. A strain numbered YM29 w...

Embodiment 2

[0047] Embodiment two: different mediums are to Staples ( Stappia sp. ) Effect of YM29 on Degradation of Zearalenone

[0048] Pick the genus Staples ( Stappia sp. ) The fresh bacterial lawn of YM29 was inoculated into LB liquid medium, and cultured on a shaker at 120r / min and 30°C for 24h to obtain seed liquid; the seed liquid was inoculated at 2% in different culture medium containing 10μg / mL zearalenone Medium, 120r / min, 30°C for 120h, the growth and degradation efficiency of the strain YM29 was determined.

[0049] The test results can be found in the appendix figure 2 As shown, the strain YM29 grew best on LB medium, and its zearalenone degradation rate was also the highest, while the strain was also grown on tryptone soy broth (TSB) and nutrient broth (NB). Better, but the degradation rate of zearalenone is lower; the growth and degradation ability of the strain in sea salt broth medium (BD) are poor.

Embodiment 3

[0050] Embodiment three: different cultivation temperature is to Staple's genus ( Stappia sp. ) Effect of YM29 on Degradation of Zearalenone

[0051] Pick the fresh bacterial lawn and inoculate it into the LB liquid medium, culture it on a shaker at 120r / min and 30°C for 24 hours to obtain the seed liquid; inoculate the seed liquid at 2% in LB medium containing 10 μg / mL zearalenone In the medium, at different temperatures, 120r / min, cultured for 120h, the growth and degradation efficiency of strain YM29 were determined.

[0052] The test results can be found in the appendix image 3 As shown, although the strain can grow at all experimental temperatures, it grows better at 30°C−37°C, and the OD value of the bacterial solution is higher. Within 20℃−37℃, the degradation rate was positively correlated with temperature, and the degradation rate gradually decreased with the increase of temperature up to 42℃.

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Abstract

The invention provides a novel strain Stappia sp.YM29 CGMCC No. 17759 and its application. The present invention screens out a strain of Stappia sp. YM29 CGMCC No.17759 in the rhizosphere soil of halophytes in a Wulapo area of Xinjiang, the isolated strain has the characteristics of salt tolerance, wide growth temperature and easy cultivation. A liquid fermentation broth of the strain can effectively degrade zearalenone in a culture medium, has remarkable and stable technical effects in the biodegradation application of zearalenone, and has wide application value for an application technologyof microbial strains.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to the technical field of a strain of the genus Staples and its application in the degradation of zearalenone. Background technique [0002] Zearalenone (Zearalenone, ZEN), also known as F-2 toxin, molecular formula C 18 h 22 o 5 , is a relatively common mycotoxin in cereal crops. Its pollution will not only directly affect the growth of crops, but also bring harm to animals, and will be directly or indirectly introduced into the human body through the food chain, seriously endangering human health. Zearalenone has reproductive toxicity, immunotoxicity, genotoxicity, and carcinogenicity. In addition, it is also toxic to the heart, kidney, liver, and lung, and it will also affect the nervous system and blood system, which will lead to hyperactivity of the nervous system. Visceral organ hemorrhage, also can cause body death when serious. Zearalen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/01
CPCA23L5/28C12N1/205C12R2001/01
Inventor 唐琦勇张志东楚敏顾美英宋素琴孙建朱静陈禹竹
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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