58 results about "Bioremediation" patented technology

This invention discloses an in-situ three-dimensional bioremediation method for slightly polluted water bodies. The method involves collecting a mud-water mixture from the water body to be treated, enriching and culturing it, and collecting the settled sludge. Culture medium II is added to the settled sludge for acclimatization and cultivation to prepare a biological agent. This biological agent is then added to a mixed solution of polyvinyl alcohol-hydrated calcium silicate-fulvic acid (PVA-CSH-FA) or PVA-modified zeolite-FA to prepare an immobilized biological carrier that can slowly release or adsorb calcium ions from the water. Calcium peroxide packing is also prepared to provide oxygen. The immobilized biological carrier packing and calcium peroxide packing are respectively fixed in the upper and lower layers of an ecological sedimentation bed, which is then placed in the slightly polluted water body to achieve in-situ three-dimensional bioremediation. The calcium-precipitating bacterial biological agent obtained through enrichment and acclimatization in this invention, when immobilized and mixed with the carrier material, efficiently and simultaneously removes pollutants such as nitrogen, phosphorus, organic matter, and heavy metals from slightly polluted water bodies.

The present application belongs to the field of microbial technology, and particularly relates to a new bacterial strain of Acinetobacter sp. and its use. The bacterial strain is isolated from mangrove soil, is a new species of Acinetobacter sp., is named as Acinetobacter sp. GXMZU3951, and the bacteria have been registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO: 36553. The Acinetobacter sp. GXMZU3951 of the present application has iron carrier production activity, can produce higher indole acetic acid, and has the ability to promote plant growth and the potential to resist heavy metals. The present application can be used in the biological remediation of heavy metal contaminated soil and as a green and environment-friendly biofertilizer for improving crop production, and has a good development and application prospect.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.

This invention discloses a method for transporting *Pterygodon spp.* for benthic bioremediation, including pre-transportation pretreatment, low-temperature transportation, and post-transportation adaptation and domestication. Before transportation, by withholding feed and stimulating the expulsion of mud and sand by low osmotic pressure, the *Pterygodon spp.* effectively induces the *Pterygodon spp.* to contract its body segments, actively expel mud and sand to clean its intestines, and achieve intestinal and body surface purification, avoiding mechanical damage and water quality deterioration risks caused by mud and sand residue in the body during transportation. After transportation, adaptation and domestication can significantly reduce the *Pterygodon spp.*'s transportation stress response, reduce body segment damage caused by violent twisting, and induce the *Pterygodon spp.* to burrow naturally by creating a suitable substrate environment and tidal environment, quickly restoring its burrowing and benthic lifestyle.

This invention relates to the field of bioremediation technology for environmental pollutants, specifically to a method for degrading side-chain-less polycyclic aromatic hydrocarbons (PAHs) using the dual-oxygenation function of polycopper oxidases. This invention is the first to discover and demonstrate that polycopper oxidases, without the need for exogenous mediators, possess the ability to catalyze the dual-oxygenation reaction of side-chain-less PAHs, directly adding two oxygen atoms from molecular oxygen to the aromatic ring of PAHs to generate the corresponding dihydroxylated intermediate. Under further catalysis by polycopper oxidase, this intermediate selectively undergoes ring-opening cleavage at specific carbon-carbon bonds in the aromatic ring via a free radical mechanism, ultimately generating low-toxicity, readily bioavailable monobenzene ring or straight-chain ester compounds. The method provided by this invention is mild, environmentally friendly, and achieves thorough degradation, offering a highly efficient and green new technology for the bioremediation of PAH-contaminated soil, water bodies, and industrial waste.

This invention discloses *Paenibacillus alvei* CHWJ-P3 for dissolving phosphorus and potassium in acidic soils and its applications. *Paenibacillus alvei* CHWJ-P3 is classified as *Paenibacillus alvei* CHWJ-P3 and was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. The *Paenibacillus alvei* CHWJ-P3 of this invention exhibits a significant ability to dissolve phosphorus and potassium in the environment, along with high acid resistance and efficient pH regulation. It can be used for the release of insoluble phosphorus and potassium in soil, i.e., the bioremediation of phosphorus and potassium dissolution in acidic soils; and for the bioremediation of acidic soil pH regulation. It features high remediation efficiency, stable remediation effects, and no secondary pollution, demonstrating promising application prospects.

This invention discloses a salt-tolerant, alkali-tolerant, and hydrocarbon-loving composite functional microbial agent for the remediation of oil-containing saline-alkali land, its preparation method, and its application. It relates to the field of environmental microbiology and soil bioremediation technology. The composite microbial agent is composed of halophilic Bacillus, alkaliphilic Pseudomonas, and petroleum hydrocarbon-degrading Mycobacterium, compounded in a viable count ratio of 3:2:2, with a single strain having a viable count ≥ 5.0 × 10⁻⁶. 9 This invention, with a concentration of CFU / g, can tolerate extreme environments including 12% NaCl concentration and pH 9.5. Through targeted screening and precise formulation of functional bacterial strains, it achieves a synergistic effect of salt and alkali tolerance and broad-spectrum petroleum hydrocarbon degradation. It can be widely applied in oilfield ecological restoration projects, recultivation of saline-alkali polluted farmland, and in-situ bioremediation of oil- and salt-alkali co-contaminated soils. The preparation process is simple, and the bacterial cell activity retention rate is high, solving the technical problems of poor stress resistance and low degradation efficiency of traditional degrading agents. It possesses excellent remediation effects and industrial application value.

A Magnetospirillum gryphiswaldense strain MSR-1, and a culture method and use thereof are provided, belonging to the technical field of environmental microorganisms. Provided is a Magnetospirillum gryphiswaldense strain MSR-1 with a deposit number of CCTCC NO: M 20242494. Conditions for culturing the Magnetospirillum gryphiswaldense strain MSR-1 are optimized, including adjusting key factors such as different carbon sources, different nitrogen sources, different iron sources, and different reducing agents, thereby simultaneously improving a biomass and a magnetic production activity of the strain MSR-1. Provided is use of the Magnetospirillum gryphiswaldense strain MSR-1 in bioremediation of cadmium polluted water and soil, thereby providing a new idea for solving the increasingly serious environmental problem of heavy metal pollution.

The application relates to application of Rhodococcus in degradation of benzene[a]pyrene in a water body and belongs to the technical field of microbial engineering. A Rhodococcus activation strain is subcultured with LB liquid culture medium, the cell concentration is adjusted, and the strain is inoculated into liquid culture medium containing benzene[a]pyrene at a volume ratio of 5%, the final concentration of benzene[a]pyrene in the culture medium is 0.5 mM; and the sample is cultured in a shaking table under the conditions that the temperature is 27 DEG C, the pH is 7.0, and the rotating speed is 180 rpm for 5 days. The Rhodococcus can grow and metabolize by taking benzene[a]pyrene as the only carbon source and energy source, and the benzene[a]pyrene can be efficiently degraded. Through systematic optimization of the degradation conditions, the optimal process parameters are determined, the degradation efficiency is obviously improved, and the application has the advantages of low cost, no secondary pollution, high degradation efficiency and the like. The application provides an efficient and environment-friendly technical scheme for biological remediation of benzene[a]pyrene pollution in a water body, and has good ecological benefits and wide application prospect.

This invention relates to a bacterium that degrades chlorobenzene pollutants and its applications. The bacterium is a *Pseudomonas putida* strain, named *Pseudomonas putida* BS-1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2024441 on March 11, 2024. Compared with existing technologies, the strain provided by this invention can completely degrade a complex pollutant mixture of dichlorobenzene, chlorobenzene, and benzene within 48 hours. It can survive for a long time and maintain its degradation activity under actual pollution conditions of complex pollution and low temperatures. Furthermore, the functional genes related to the degradation of chlorobenzene pollutants are located on a plasmid, which helps the indigenous bacterium acquire the relevant functions for degrading chlorobenzene pollutants. This strain can be applied to in-situ bioremediation of groundwater with complex chlorobenzene pollution in chemical industrial parks, effectively overcoming the technical shortcomings of traditional reduction dechlorination remediation technologies, which often result in incomplete reduction of products, and achieving green, sustainable, and thorough purification without secondary pollutants.

This invention discloses a highly efficient strain of *Bacillus tekirii* BB that degrades PLA microplastics and its applications. This strain was deposited on February 2, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing, with accession number CGMCC NO. 37630. This strain can secrete multiple enzyme systems, including lipase, amylase, laccase, and catalase. In soil systems, this strain can effectively degrade PLA, increase soil lactic acid content and the activity of various soil enzymes, and improve soil organic matter, nitrogen, phosphorus, and potassium nutrient status. This strain can alleviate the growth stress of PLA pollution on plants, increasing the plant height, biomass, and antioxidant enzyme activity of crops such as buckwheat. This strain combines PLA degradation, soil remediation, and plant growth promotion functions, making it suitable for in-situ bioremediation of PLA microplastic pollution in farmland and possessing significant environmental and agricultural application value.

The present application is suitable for the field of bioremediation technology, and provides a bioremediation preparation for heavy oil pollution, which comprises an immobilized heavy oil degrading bacterial population preparation, an immobilized laccase preparation, a slow-release nitrogen and phosphorus nutrient preparation, and a rhamnolipid surfactant; the bioremediation preparation is a solid preparation. The present application also provides application of the bioremediation preparation in a heavy oil pollution intertidal zone. The bioremediation preparation has the advantages of not being easy to lose, fast bioremediation starting speed, being superior in competition with indigenous bacterial species, and good environmental tolerance, thereby overcoming the problems of slow starting speed and fast failure speed of traditional bioremediation preparations in remediation of heavy oil pollution intertidal zones.

The application relates to a method and device for full-amount biological soil bioactivity reclamation of red mud by a biological method, wherein the method comprises the following steps: S10, mixing cane pith powder and red mud at a certain ratio; S20, feeding livestock and poultry manure and first compound formula strains into a biological reactor, continuously feeding sterile air and keeping a certain temperature and pressure, forming internal circulation of fermentation substrates under the action of air flow and a flow guide cylinder, and becoming acidic substances under the action of microbial bacteria; S30, carrying out neutralization reaction of the acidic hydrolyzate and the red mud in a neutralization waste heat recovery reaction tank, outputting in the form of mud slurry, and carrying out pressure filtration to generate filter mud; S40, adding soil conditioner or bio-organic fertilizer and second compound formula strains to the filter mud, and improving the organic matter content and soil bioactivity; and S50, completing soilization under the combined action of multiple microorganisms. The technical scheme of the application solves the problems of waste of land resources and environmental pollution caused by red mud stacking in the prior art.

The utility model relates to soil vanadium microbial repair technical field especially relates to a soil vanadium microbial repair box. Its technical scheme includes the box, the box top fixedly connected with motor, motor output fixedly connected with drive cover, drive cover rotation is connected in the box inside, drive cover inside is away from motor one end sliding connection has the lift pole. The utility model drives the lift pole to rotate when drive cover rotates, and the lift pole will rely on the adjusting rod and slide in the sliding groove, thereby realizing the lift pole rotation lift, thereby let the lift pole drive the rotating rod and the stirring board carry out the effect of mixing while stretching and contracting, can better mix soil and microorganism, and the soil is also scattered, can provide the pushout force when soil is discharged, avoids the soil hard, cannot scatter in the stirring board gap position, and the problem that the adhesion is bigger and is not convenient to discharge, improves soil mixing effect and soil discharge unobstructed effect.

The utility model relates to crack repair technical field, and disclose a kind of high-efficiency repair device for microbial repair concrete crack, including base and bucket, the bucket fixed mounting is at the top of base, the bucket top fixed mounting has top cover, first mounting sleeve is fixedly inserted on the top cover, connecting rotary drum is rotatably inserted on the first mounting sleeve, second shaft is inserted in the connecting rotary drum, second mounting sleeve is fixedly installed at the top and bottom of connecting rotary drum respectively, the second shaft is rotatably inserted in two second mounting sleeves respectively, ensure that microbial bacteria and cementing component are uniformly mixed, maintain slurry component stability, uniform slurry environment can guarantee microbial activity, promote its metabolic production calcium carbonate and other cementing material efficiency, improve crack filling density, effectively improve the practicality of the device.

The present application belongs to the field of microbial technology, and relates to a high-efficiency dichloroacetic acid degrading Pseudomonas aeruginosa and application thereof, the high-efficiency dichloroacetic acid degrading Pseudomonas aeruginosa is named SJTX, and the preservation number is CGMCC No. 36706. The Pseudomonas aeruginosa SJTX in the present application can grow by using dichloroacetic acid as a sole carbon source, can efficiently degrade dichloroacetic acid, and the degradation rates of the Pseudomonas aeruginosa on 10 mg / L, 50 mg / L and 100 mg / L dichloroacetic acid within 24 hours are 45.8%, 42.4% and 24.4% respectively, and the Pseudomonas aeruginosa has good adaptability to high salt and heavy metals, and is suitable for effectively removing and bioremediating dichloroacetic acid pollutants in water bodies, soil and other environments. The strain of the present application has good comprehensive performance in the degradation efficiency of dichloroacetic acid and environmental tolerance compared with the dichloroacetic acid degrading strains found.

The utility model relates to wall body crack repair technical field, specifically disclose the device of crack of wall body is repaired with microorganism, including the crack sealing grouting chamber for grouting, the telescopic support frame of one side of crack sealing grouting chamber installation, the one side of crack sealing grouting chamber is provided with infusion grouting pipeline, the infusion grouting pipeline is used for cooperating crack sealing grouting chamber to carry out grouting filling to crack, the crack sealing grouting chamber is the triangle structure, and one side is provided with double -deck grouting angle adjusting disc, one side of double -deck grouting angle adjusting disc with one side of telescopic support frame is adapted, the utility model discloses device need not to adopt the paste structure and wall body connection, avoid the scratch, peeling etc. damage caused to the wall surface because of the removal of paste material, especially applicable to the historical building or protective structure of higher requirement to the wall surface integrity.

This invention discloses an Acinetobacter DL27 strain and its application in the degradation of acetaminophen, belonging to the field of microbial technology. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38039, deposited on April 3, 2026, and taxonomically named Acinetobacter. Acinetobacter sp . This invention has screened a strain of Acinetobacter DL27 that can efficiently degrade acetaminophen, and it can efficiently degrade acetaminophen in wastewater from actual sewage treatment plants and agricultural soils, which has important application value in the bioremediation of acetaminophen in complex environments.

This invention proposes a method for reducing cadmium in paddy fields using common soil microorganisms, belonging to the field of agricultural technology. Addressing the problems of high cost, secondary pollution, and soil structure damage associated with existing physicochemical cadmium reduction methods, this invention provides a bioremediation technology using Bacillus subtilis ATCC6633 as the functional strain. This method involves activating laboratory-preserved Bacillus subtilis ATCC6633 bacterial powder to prepare a microbial agent, which is then directly applied to the surface water of paddy fields. Utilizing the specific adsorption and conversion of cadmium ions by the strain, effective passivation and removal of cadmium from paddy fields are achieved. This invention uses common soil microorganisms, exhibiting strong environmental compatibility and eliminating the need for chemical agents, fundamentally avoiding the secondary pollution problems caused by traditional chemical precipitation methods. It is both eco-friendly and economically feasible, significantly improving rice quality and providing an effective green remediation approach for cadmium pollution in paddy fields.

The application discloses application of a broad-spectrum type III esterase derived from Rhodococcus in phthalate ester pollution bioremediation, and overcomes the limitation of low degradation efficiency of a natural strain; through genetic engineering technology, the esterase 5359 gene is cloned into a Rhodococcus-E.coli shuttle vector pNV18 to construct a recombinant Rhodococcus engineering bacterium WT-pNV18-5359 overexpressing the esterase. Experimental results show that the engineering bacterium significantly improves the degradation performance: in single PAEs degradation test, the degradation rates of long-chain PAEs such as DEHP, DnOP and DiNP are respectively increased by 15.71%, 13.99% and 12.82% compared with the wild type; in a mixed PAEs system, the degradation of long-chain PAEs is obviously superior, and the engineering bacterium has good genetic stability and does not affect growth. Through genetic engineering to strengthen the application of known enzymes, efficient and stable PAEs pollution bioremediation is realized.

The application discloses a method for bioremediation of improved saline-alkali soil, comprising the following steps: step one, obtaining a bacteria-enzyme compound preparation; step two, obtaining pretreated seedling nutrient soil; step three, cultivating to obtain salt-tolerant plant seedlings; and step four, completing the treatment and improvement of the saline-alkali soil. The method is based on the traditional mode, and utilizes microbial preparations, enzyme preparations and substrates to strengthen seedling transplanting, so that the treatment effect is improved, the seedling rate and survival rate are improved, and the saline-alkali soil is quickly and effectively treated and improved.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

This invention discloses an aromatic compound transporter protein mutant and its applications, belonging to the field of bioengineering technology. The mutant is based on *Pseudomonas putida* (…). Pseudomonas putida The outer membrane transport protein derived from [a specific source] is obtained by site-directed substitution of key residues in its gating loop. Specifically, this includes replacing phenylalanine at position 120 with glycine, leucine at position 121 with glycine, and glutamine at position 122 with glycine, or one or more combinations thereof. Preferred mutants include single mutant F120G, double mutant F120G / L121G, or triple mutant F120G / L121G / Q122G, and conserved substitution derivatization can also be performed based on these mutants. These mutants can be used for the efficient microbial production and cell tolerance regulation of aromatic compounds, based on the physicochemical properties of the target product, and have significant application value in green biomanufacturing, fermentation engineering, and bioremediation.