281 results about "Bioremediation" patented technology

The invention discloses ultra-high performance concrete and a preparation method thereof. The ultra-high performance concrete comprises the following components in percentage by mass: 15%-25% of cement, 3%-6% of nano SiO2, 30%-35% of fly ash or steel slag powder, 35%-40% of recycled aggregate, 1%-4% of fiber, 0.5% of a microbial remediation agent and 1%-3% of nano TiO2. The preparation method comprises the following steps: S1, pretreating the recycled aggregate; and S2, packaging the microbial remediation agent: mixing the bacillus pasteurii spores with calcium lactate, and wrapping the mixture in calcium phosphate microspheres. S3, step-by-step stirring; S3.1, dry mixing: uniformly stirring the cement, the fly ash, the nano SiO2, the nano TiO2 and the recycled aggregate; s3.2, wet mixing is carried out, water solution fibers containing the polycarboxylic acid water reducing agent are added, uniform stirring is carried out, and the water-binder ratio of the water solution containing the polycarboxylic acid water reducing agent is 0.18; s3.3, final mixing: adding the packaged microbial remediation agent, and uniformly stirring; and S4, directional curing: performing steam curing at 80 DEG C for 48 hours, performing ultraviolet irradiation, and performing constant-temperature and constant-humidity curing for 7 days. The ultra-high performance concrete has the effects of low cost, low carbon emission, multi-function integration and repeatable crack self-repairing.

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention belongs to the technical field of environmental protection of bioremediation wastewater, and discloses an immobilized adsorption microsphere, which is prepared from Bacillus spp., Candida utilis and European nitrosomonas through an immobilized embedding technology. The microspheres provided by the invention can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The invention discloses a Gordonia ovorans YDY-4 strain and an application of the Gordonia ovorans YDY-4 strain in degradation of n-hexadecane. The cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 20251290, and the cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4 and is preserved in the China Center for Type Culture Collection (CCTCC). The Gordonia ovorans YDY-4 provided by the invention has the capability of degrading n-hexadecane under the condition that the pH (Potential of Hydrogen) is 7.0 to 9.0, and can grow at 28 to 32 DEG C. The Gordonia ovorans YDY-4 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a basis for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The invention discloses a method for biologically repairing soil by using crude oil degrading fungi, and belongs to the technical field of microorganisms and biology. The method comprises the following steps: step 1, directionally screening strains of the crude oil degrading fungi; step 2, function enhancement of the screened strains; 3, preparing a repair carrier; and 4, carrying out gradient process and composite flora synergistic remediation. According to the method, indigenous fungi are screened and separated in a crude oil pollution area, the screened strains are subjected to adaptive laboratory evolution, the degradation capacity and the environmental endurance capacity are enhanced, a preparation process of composite microspheres is combined, adsorption-degradation efficient remediation of crude oil pollution is achieved, and a gradient process and other biological methods are used for cooperating remediation measures; the remediation effect is further enhanced, the natural ecological environment is stably restored, secondary pollution in the remediation process is avoided, and the risk of ecological pollution is reduced.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

The invention relates to the field of environmental microbial remediation, in particular to a strain of trichlorobacterium and application thereof in bioremediation of pollutants. According to the present invention, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, and the bacillus subtilis is bacillus subtilis. The strain NB-12 obtained by the invention is a strain of self-synthesized cobalamin screened from river bottom sediments, is suitable for extensive industrial production and degrades a plurality of halogenated alkanes in the environment.

The invention provides bacillus mycoides Y2 with functions of reducing cadmium content of crops and promoting growth and application of the bacillus mycoides Y2, and relates to the technical field of soil heavy metal pollution bioremediation and agricultural microorganism application. The bacillus mycoides Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2025, and the preservation number is CGMCC 35231. The strain can be applied to cadmium-polluted soil through root application, can reduce the cadmium content of edible parts of crops such as rice, oilseed rape and fast vegetables, and promotes crop growth and rice root surface iron plaque formation. The contaminated soil in Hunan, Henan and Tianjin is verified to be capable of stably colonizing and adapting to different soils and crops. The strain and the matched preparation are green, efficient, high in stability and wide in universality, and provide key technical support for safe utilization of cadmium-polluted cultivated land.

The invention discloses a salicylic acid decarboxylase mutant and application of the salicylic acid decarboxylase mutant in degradation of salicylic acid. The mutant is obtained by carrying out site-specific modification on salicylic acid decarboxylase NahG, and specifically, valine at the 46th site, tyrosine at the 380th site and leucine at the 382nd site of an amino acid sequence shown in SEQ ID NO.2 are mutated into cysteine, phenylalanine and phenylalanine at the same time. The mutant shows remarkably improved catalytic efficiency, and salicylic acid can be efficiently and thoroughly decarboxylated and converted into catechol under mild conditions. The invention also provides a coding gene, a recombinant vector and an engineering bacterium of the mutant. The mutant not only can be used for efficient bioremediation of salicylic acid pollution, but also can effectively break through and accelerate a naphthalene catabolism pathway due to the characteristic of directionally generating catechol, and has important application value in the fields of environmental pollution abatement and biological catalysis.

The invention relates to the technical field of soil remediation, in particular to an efficient in-situ microbial remediation method for organic contaminated soil, which comprises the following steps: layering and dividing the contaminated soil into layers and regions, and injecting different medicaments into circulating well groups positioned in different regions to achieve the purpose of adjusting an underground microbial environment, and automatically forming and constructing an aerobic-anaerobic transition zone. Meanwhile, self-circulation of underground water is accelerated through the circulating well group, different indigenous microorganism florae are activated, and a metabolic chain is formed; and aerobic / anaerobic microorganisms are periodically injected into the injection well, an electron donor and a carbon source are synchronously provided, the steady state of an ecosphere is maintained, and the aims of optimizing the construction period and the treatment effect of the in-situ bioremediation technology of the polluted soil and the underground water are achieved.

The invention discloses an aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83 and application thereof, the strain is classified and named as Pseudomonas protegens, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.36443. The invention further discloses a preparation method of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The invention further discloses an application of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The strain has outstanding ammonia nitrogen metabolism capability under an aerobic condition, can quickly and efficiently convert ammonia nitrogen in a water body into a harmless or low-toxicity form, has wide tolerance and adaptability to environmental factors such as pH, temperature and the like, and is stable in performance. The strain is suitable for bioremediation of natural water, domestic sewage and various industrial wastewater, can effectively improve the denitrification efficiency and the system stability, and has the advantages of simplicity and convenience in operation, environmental friendliness and high practical application value.

The invention discloses bacillus cereus with wide pH adaptability and application of the bacillus cereus in soil or underground water heavy metal remediation, the strain is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20241939), and the bacillus cereus is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (CCTCC). The strain is high in environmental adaptability and high in remediation efficiency, can show good reduction and endurance capacity to various heavy metal pollutants under the wide environmental conditions that the pH ranges from 4 to 10 and the temperature ranges from 16 DEG C to 37 DEG C, and is particularly suitable for in-situ remediation of high-concentration contaminated soil; experimental results represented by hexavalent chromium show that the removal rate of the strain in 1000 mg / kg hexavalent chromium contaminated soil within 7 days can reach 70-100%, the dependence of an existing microbial remediation technology on a low-concentration and alkaline environment or a long remediation period is broken through, and an efficient solution is provided for green bioremediation of multiple types of heavy metal contaminated sites.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

This invention discloses an aquatic microbacterium and its application in the degradation of atrazine. The aquatic microbacterium is named *Aquamicrobium lusatiense* HR8, with accession number CGMCC No. 28916. This invention also discloses a microbial agent comprising the aquatic microbacterium, a method for culturing the aquatic microbacterium, and a method for degrading triazine herbicides. This invention is the first to disclose the use of *Aquamicrobium* to degrade atrazine. Aquamicrobium HR8 achieved a 100% degradation rate of 200 mg / L atrazine in 48 hours. Its application in the bioremediation of atrazine-contaminated environments can overcome the current shortcomings of poor atrazine bioremediation and removal efficiency, while simultaneously reducing secondary pollution to the environment.

This invention discloses an in-situ three-dimensional bioremediation method for slightly polluted water bodies. The method involves collecting a mud-water mixture from the water body to be treated, enriching and culturing it, and collecting the settled sludge. Culture medium II is added to the settled sludge for acclimatization and cultivation to prepare a biological agent. This biological agent is then added to a mixed solution of polyvinyl alcohol-hydrated calcium silicate-fulvic acid (PVA-CSH-FA) or PVA-modified zeolite-FA to prepare an immobilized biological carrier that can slowly release or adsorb calcium ions from the water. Calcium peroxide packing is also prepared to provide oxygen. The immobilized biological carrier packing and calcium peroxide packing are respectively fixed in the upper and lower layers of an ecological sedimentation bed, which is then placed in the slightly polluted water body to achieve in-situ three-dimensional bioremediation. The calcium-precipitating bacterial biological agent obtained through enrichment and acclimatization in this invention, when immobilized and mixed with the carrier material, efficiently and simultaneously removes pollutants such as nitrogen, phosphorus, organic matter, and heavy metals from slightly polluted water bodies.

This invention discloses a halophilic fast-growing bacillus strain that degrades chlorinated persistent organic pollutants and its applications. The strain is *Celeribacter baekdonensis* TBC005, with accession number GDMCC No:65236. The *Celeribacter baekdonensis* TBC005 of this invention exhibits excellent degradation ability against chlorinated persistent organic pollutants. After 14 days of anaerobic culture, it shows significant degradation of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP reached 21.5%, 16.7%, and 16.9%, respectively; after 35 days of cultivation, the degradation rates of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP can reach 50.7%, 59.2%, and 61.3%, respectively, and they can be applied to the environmental pollution control and bioremediation of chlorinated persistent organic pollutants.

The invention belongs to the technical field of heavy metal contaminated soil bioremediation, and particularly relates to a method for remediating heavy metal contaminated soil through a Fe3C-N-S composite modified electrode enhanced bioelectrochemical system. Graphene oxide, an iron source, a nitrogen source and a sulfur source serve as raw materials, a Fe3C-N-S composite material is prepared through high-temperature calcination, then the Fe3C-N-S composite material is compounded with sodium alginate (SA) hydrogel, the surface of a graphite rod is coated with the composite material, a Fe3C-N-S composite modified electrode is constructed through a CaCl2 cross-linking and freeze-drying technology, then the modified electrode is coupled with a graphite cathode, and the composite material is obtained. And the acidophilic thiobacillus ferrooxidans is combined to construct a bioelectrochemical system, so that the synergistic and efficient leaching of the heavy metals in the soil is realized. The conductivity, biocompatibility and corrosion resistance of the electrode can be remarkably improved, the problems that mass transfer is difficult and microorganism loading is unstable in BES remediation are solved, and a breakthrough technical scheme is provided for green and efficient remediation of heavy metal contaminated soil.

The application discloses a sulfonylurea herbicide hydrolytic enzyme TsmE mutant, a coding gene thereof and a use thereof. The amino acid sequence of the mutant is shown in SEQ ID NO. 4. The mutant M6 provided by the application has deesterification activity to macromolecular sulfonylurea herbicides methyldisulfuron and sodium salt of methyl iodosulfuron, and therefore has a good application prospect in sulfonylurea herbicide residual pollution bioremediation and anti-herbicide transgenic engineering.

The present application relates to the field of microbial technology, and more particularly to a kind of for degrading high-efficiency fluazifop-butyl ester urea serratia DHPM01 and its application, urea serratia DHPM01 is preserved in China Microbial Culture Collection Center on September 18, 2025, preservation number is CGMCC NO.35971, and the address of preservation is Beijing Chaoyang District Beichen West Road No.1, and the classification designation is Serratia ureilytica The microbial agent provided by the present application can effectively degrade high-efficiency fluazifop-butyl ester in a wide pH and temperature range, and urea serratia DHPM01 can be used as an excellent pesticide-degrading bacterium and widely applied to the bioremediation of high-efficiency fluazifop-butyl ester residues in water, farmland and contaminated soil and other environments, providing a new efficient and environmentally friendly strategy for pesticide pollution control and enriching the germplasm resource bank of pesticide-degrading functional microorganisms.

A method for organic compound removal from a solution using microalgae includes extracting one or more hydrocarbons from an underground geologic formation to form a hydrocarbon composition and a produced water including at least a portion of the one or more hydrocarbons and one or more salts. The method further includes mixing the produced water with a wastewater to form a solution, and then contacting a microalgae feedstock, Chlorella sorokiniana, with the solution in a bioreactor. The total lipid content of the microalgae feedstock is 13 to 17%. The method includes removing the one or more hydrocarbons, nitrogen, and phosphorous from the solution by algal consumption with the microalgae feedstock. The method of the present disclosure removes 80 to 90 wt. % of the one or more hydrocarbons, 70 to 85 wt. % nitrogen, and 90 to 100 wt. % phosphorous.

The present application belongs to the field of microbial technology, and particularly relates to a new bacterial strain of Acinetobacter sp. and its use. The bacterial strain is isolated from mangrove soil, is a new species of Acinetobacter sp., is named as Acinetobacter sp. GXMZU3951, and the bacteria have been registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO: 36553. The Acinetobacter sp. GXMZU3951 of the present application has iron carrier production activity, can produce higher indole acetic acid, and has the ability to promote plant growth and the potential to resist heavy metals. The present application can be used in the biological remediation of heavy metal contaminated soil and as a green and environment-friendly biofertilizer for improving crop production, and has a good development and application prospect.

This invention belongs to the field of environmental microbiology technology, specifically disclosing a *Aspergillus niger* strain with lead adsorption capacity and its applications. This invention involved the isolation of microorganisms from 33 samples of water and sediment from the Henan section of the Yellow River basin. A fungal strain with high lead tolerance and adsorption was screened out, and after morphological and ITS molecular identification, it was named *Aspergillus niger* AN-2 and deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC) with accession number GDMCC NO: 63697. Compared with other isolated strains, *Aspergillus niger* AN-2 exhibits high lead tolerance and adsorption capacity for Pb. 2+ It has the strongest tolerance and is most tolerant to Pb. 2+ The MIC is as high as 3500-3800 mg / L. When this strain is processed into biomass, it exhibits excellent adsorption performance for lead, making it suitable for preparing bioremediation agents for heavy metal pollution. It has broad application prospects and high economic value.

The invention relates to the technical field of environmental remediation microorganisms, and discloses a mixed polycyclic aromatic hydrocarbon degrading bacterium G3 suitable for variable environments and application thereof. The bacterial strain is pseudoxanthomonas sp. G3, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20251828. The strain G3 is enriched and separated from soil of a certain coking plant in Beijing, can utilize polycyclic aromatic hydrocarbons such as acenaphthene, fluorene, phenanthrene, fluoranthene and pyrene as a single carbon source, and can simultaneously degrade acenaphthene, fluorene, phenanthrene, fluoranthene and pyrene mixtures within 14 days, so that the degradation rate of each component is not lower than 70%; good growth and degradation activity are maintained under the conditions that the temperature is 20-37 DEG C, the salinity is 0-4% NaCl and the pH value is 5.0-9.0, and the degradation rates can reach 100% and 84.15% respectively after the strain is cultured in a single carbon source culture solution with the initial concentration of phenanthrene and pyrene being 50 mg.L <-1 > for 7 days; after the polycyclic aromatic hydrocarbon aging polluted soil is cultured for 28 days, the mixed polycyclic aromatic hydrocarbon in the soil can be effectively removed. According to the invention, the technical bottlenecks of poor environmental adaptability and low mixed pollutant degradation efficiency of the existing degrading bacteria are solved. Therefore, the strain has good application potential in the aspect of bioremediation of the polycyclic aromatic hydrocarbon polluted environment.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The present invention discloses a microbial remediation method for soil in rare earth heap leaching mines. The method comprises the following steps: mixing a composite microbial agent, distilled water, sucrose, potassium dihydrogen phosphate, magnesium sulfate, and sodium chloride to obtain a mixed solution A; adding modified zeolite to the mixed solution A and stirring to obtain a mixed solution B; adding chitosan to an acetic acid aqueous solution, followed by adding polyvinyl alcohol and stirring to obtain a mixed solution C; mixing Span 80 and liquid paraffin uniformly and heating to 40-50°C; then adding the mixed solution C under stirring; and then sequentially adding the mixed solution B and glutaraldehyde to the mixture and continuing to stir to obtain slow-release microspheres; mixing calcium hydroxide, water, polyethylene glycol, and the slow-release microspheres to obtain a soil conditioner; rinsing the mine soil with water for 1-2 days, turning the soil when the weather is clear, then drying it for 1-2 days and leveling the land, and then spraying the soil conditioner onto the soil. The present invention is suitable for the improvement and remediation of soil in rare earth heap leaching mines and has the characteristics of high applicability and good improvement and remediation effect.

The invention discloses a nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1) with a capability of degrading multiple pollutants and an application of the nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1, and belongs to the technical field of screening and application of functional microorganisms. The strain is derived from a chicken manure aerobic composting environment, the preservation number is GDMCC No: 67351, and nitro polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons and tetracycline antibiotics can be efficiently degraded at the same time. In a single pollutant with the concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceed 97% in 8 h, the degradation rate of nitrofluoranthene reaches 99.01% in 48 h, and the degradation rate of phenanthrene reaches 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the 48-hour degradation rate is kept to be 78% or above. The strain is high in degradation efficiency and strong in environmental adaptability, provides a new microbial resource for bioremediation of a combined pollution site, and has a wide application prospect.

This invention discloses a composite nanofiber membrane for immobilizing microorganisms to remediate diesel-contaminated water and its preparation method. Specifically, the composite nanofiber membrane is a thermoplastic polyurethane / nanohydroxyapatite composite nanofiber membrane, which not only possesses excellent biocompatibility and mechanical strength but is also non-toxic and harmless, offering considerable cost-effectiveness. Upon introduction into diesel-contaminated water, the composite nanofiber membrane first rapidly adsorbs diesel at the water / oil interface, preventing further diffusion and toxic effects on the aquatic environment. Subsequently, due to the additional adsorption sites provided by the nanoparticles on the membrane, a large number of free bacteria are adsorbed onto the membrane, enhancing the metabolic activity of bacterial cells and their resistance to the toxicity of stubborn compounds, while also strengthening the affinity between hydrophobic compounds and cells, thereby increasing the degradation rate of diesel hydrocarbons by microbial cells. This invention improves the remediation effect of diesel wastewater while expanding the application of composite nanofiber membranes in the field of water pollution treatment.

The application discloses a soil enterobacter capable of efficiently degrading polypropylene and application thereof. The strain is FJNUIM-S2, which is classified and named as soil enterobacter (Enterobacter agglomerans) Enterobacter soli ), is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is preserved on October 17, 2024, with a preservation number of CGMCC No. 32241 and a preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Xiliujiaobei, Chaoyang District, Beijing. The strain FJNUIM-S2 of the application can grow with polypropylene as the only carbon source, has good polypropylene degradation characteristics, provides a microbial resource for bioremediation of waste polypropylene in the environment, and has a wide application prospect.