496 results about "Bioremediation" patented technology

The invention discloses an acid mine wasteland ecological restoration plant dynamic optimization configuration method, and relates to the field of ecological restoration, and the method comprises the following steps: 1, collecting soil attribute data in a predetermined range of an acid mine wasteland in a dotted manner, and synchronously obtaining growth attribute data of to-be-selected plants and periodic weather data of a target area; step 2, extracting a deterioration feature set having a deterioration effect on plant growth based on the soil attribute data, and analyzing a bioremediation feature set capable of inhibiting the deterioration feature in the plant attribute data to generate a candidate plant set; 3, constructing a dynamic environment simulation model based on a deep learning algorithm, wherein input dimensions comprise weather data time sequence features, soil deterioration feature vectors and a plant bioremediation feature matrix; the method can accurately predict the restoration effects of plants under different climate and soil conditions, simulate the effects of different intervention measures in real time, output the optimal intervention scheme, and ensure the balance between the resource utilization efficiency and the restoration effects.

The invention discloses ultra-high performance concrete and a preparation method thereof. The ultra-high performance concrete comprises the following components in percentage by mass: 15%-25% of cement, 3%-6% of nano SiO2, 30%-35% of fly ash or steel slag powder, 35%-40% of recycled aggregate, 1%-4% of fiber, 0.5% of a microbial remediation agent and 1%-3% of nano TiO2. The preparation method comprises the following steps: S1, pretreating the recycled aggregate; and S2, packaging the microbial remediation agent: mixing the bacillus pasteurii spores with calcium lactate, and wrapping the mixture in calcium phosphate microspheres. S3, step-by-step stirring; S3.1, dry mixing: uniformly stirring the cement, the fly ash, the nano SiO2, the nano TiO2 and the recycled aggregate; s3.2, wet mixing is carried out, water solution fibers containing the polycarboxylic acid water reducing agent are added, uniform stirring is carried out, and the water-binder ratio of the water solution containing the polycarboxylic acid water reducing agent is 0.18; s3.3, final mixing: adding the packaged microbial remediation agent, and uniformly stirring; and S4, directional curing: performing steam curing at 80 DEG C for 48 hours, performing ultraviolet irradiation, and performing constant-temperature and constant-humidity curing for 7 days. The ultra-high performance concrete has the effects of low cost, low carbon emission, multi-function integration and repeatable crack self-repairing.

The invention discloses a haloalkane dehalogenase mutant and an application thereof in degradation of 1, 2, 3-tribromopropane, haloalkane dehalogenase DhaA31 coded by dhaA gene of a Rhodococcus rhodococcus NCIMB 13064 strain is modified to obtain the haloalkane dehalogenase mutant F168W / I246C, the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane, and the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane under the condition of water phase normal temperature. The degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane within 1 h reaches 100%, and the degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane reaches 100%. The haloalkane dehalogenase mutant has high stereoselectivity on (R)-2, 3-dibromo-1-propanol, and the e.r. Value of the haloalkane dehalogenase mutant reaches 6: 94. The haloalkane dehalogenase mutant provided by the invention has high catalytic efficiency and high stereoselectivity, is beneficial to recognition and further degradation of downstream enzymes, and has a good application prospect in bioremediation of emerging haloalkane pollutants.

The microbial remediation ceramsite comprises microorganisms, nutrient substances, modified porous slow-release Ca < 2 + > ceramsite and a cement-based protective shell, the microorganisms and the nutrient substances are loaded in the modified porous slow-release Ca < 2 + > ceramsite, and the cement-based protective shell wraps the outer surface of the modified porous slow-release Ca < 2 + > ceramsite. The surface of the ceramsite is modified by an aggregation-crosslinking method, and the modified microbial ceramsite carrier has higher microbial loading capacity and better stability under the dual actions of physical adsorption and valence bond combination, so that the repair effect of the ceramsite on cracks is further ensured; the cement-based protective shell adopts epoxy resin, so that the cylinder compressive strength of the ceramsite is improved, and microorganisms and nutrient substances are protected from being damaged by a high-alkalinity environment; the cement-based mixed powder is selected to enhance the interface adhesive force between the ceramsite and cement concrete, the sulphoaluminate cement in the mixed powder ensures the strength of the ceramsite in the early stage, and the fly ash, the silica fume, the nano-silica and other materials enable the later strength of the ceramsite to be continuously increased.

The invention discloses a heavy metal pollution remediation material combining minerals and bacteria and a preparation and remediation method thereof. The heavy metal pollution remediation material comprises the following components in parts by mass: 50 parts of clay minerals, 30 parts of phosphate minerals, 10 parts of bacteria and the balance of organic fertilizer as a matrix, wherein the clay mineral is attapulgite, the phosphate mineral is apatite, and the bacteria are one of enterobacter. By combining the characteristics of minerals and bacteria, the remediation material has strong adsorption capacity and can effectively remove heavy metal ions in soil. Meanwhile, the enterobacter converts heavy metal ions into insoluble precipitates through a bioremediation mechanism of the enterobacter, such as adsorption, reduction, chelation and the like, so that subsequent treatment is facilitated. The metabolic activity of the enterobacter in the soil can promote the growth and activity of soil microorganisms, increase the content of beneficial elements in the soil, improve the soil structure and improve the soil quality.

The present invention discloses an alkali-resistant microbial bacterium PDC-1 and its application. The present invention screens and isolates PDC-1 strain from the soil of a coal gangue stockpile in a coal mine in Shandong, China, which can utilize naphthalene, phenanthrene, anthracene or pyrene as the sole carbon source and has tolerance to heavy metals. The strain of the present invention is capable to degrade various PAHs with the presence of heavy metals. The strain is alkali resistant, simple to cultivate, and has a good application in the bioremediation of polycyclic aromatic hydrocarbons and heavy metal contaminated sites.

The embodiment of the invention discloses a bioremediation device for organic contaminated soil. The bioremediation device comprises a photovoltaic electrolysis assembly, a mass transfer transition assembly and a soil remediation assembly. The photovoltaic electrolysis assembly generates oxygen and hydrogen through solar electrolysis of water; the mass transfer transition component is used for conveying gas to a bionic root system main body of the soil remediation component in a partitioned manner, a hydrogen injection hole is formed in the tail end of a first-stage branch to form an anaerobic micro-region, and an oxygen injection hole with a hydrophobic membrane is formed in the tail end of a second-stage branch to maintain an aerobic environment; the surface of the bionic root system is coated with a composite layer, titanium dioxide nanofibers are excited through an ultraviolet LED to generate holes to oxidize organic matter, and electrons are subjected to heavy metal reduction strengthening through a graphene grid. According to the method, the traditional microbial remediation period is shortened and the organic matters and the heavy metals are synchronously removed through triple mechanisms of gas partitioned and graded controlled release, photocatalysis-electrochemistry cooperation and in-situ electron targeted delivery, the whole process is completed in situ, earthwork excavation and outward transportation risks are avoided, and the method has the advantages of high efficiency, stability and environmental protection.

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention discloses application of a mineralized carbon sequestration strain, the mineralized carbon sequestration strain is classified and named as Halomonas sulfidaeresis. ZN2 #, the mineralized carbon sequestration strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32033. The invention further discloses a preparation method of the mineralized carbon sequestration strain. The mineralization carbon sequestration strain can metabolize to generate urease and carbonic anhydrase at the same time, the two enzymes are subjected to a dual mineralization reaction in combination with a microorganism-induced calcium carbonate precipitation technology, the crystal form of a mineralization product is improved, the mineralization strength is improved, meanwhile, carbonic anhydrase can absorb carbon dioxide in the environment, high-strength soil reinforcement is achieved, meanwhile, carbon dioxide emission is reduced, and the soil quality is improved. Therefore, the dual effects of biomineralization remediation and improvement and carbon sequestration and emission reduction of the soil are achieved, and sustainable development of a soil bioremediation and improvement technology is achieved.

The invention provides a mechano-electric coupling hydrogel microsphere with stable mechanical property and low loss transduction as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering bioremediation materials. According to the invention, supramolecular engineering and microfluidic technologies are utilized, and a slip ring structure polyrotaxane and conductive polypyrrole network is introduced to construct stress-electric coupling hydrogel microspheres. The molecular slippage mechanism of the slip ring structure stores and releases mechanical energy to reduce mechanical loss, and the conjugated pi-electron movement in the conductive network improves the electron transfer efficiency in the microsphere, so that the loss in stress-electricity conversion is reduced for the first time. Compared with the traditional piezoelectric hydrogel microspheres, the stress-electric coupling efficiency of the low-dissipation microspheres is improved by 2.3 times, and the energy dissipation rate is reduced to 43%. The mechano-electric coupling hydrogel microspheres can reduce cartilage injury of rats, improve behavior results and promote treatment of osteoarthritis by recovering low-loss transduction between tissues.

The invention discloses a Pandoraea sp. Capable of efficiently degrading aureomycin and application of the Pandoraea sp. The strain is a strain FJNUIM-SW2, the classification name of the strain is Pandoraea nomenusa, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is October 17, 2024, the preservation number is CGMCC NO.32242, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The strain FJNUIM-SW2 can grow by taking aureomycin as the sole carbon source and energy, has a good aureomycin degradation characteristic, can be used for microbial degradation of aureomycin in environments such as aureomycin wastewater and soil, provides microbial resources for bioremediation of aureomycin in the environment, and has potential application value in ecological environment protection.

The invention discloses an aquaculture non-point source pollution treatment method based on ecological-hydraulic engineering, which comprises the following steps: acquiring water quality monitoring basic data of a target aquaculture area, inputting the water quality monitoring basic data into an ecological simulation environment matched with an ecological-hydraulic engineering fishpond, and generating a plurality of ecological purification schemes, constructing an environmental fluid dynamic model corresponding to the ecological-water conservancy project fishpond by taking the lowest waterlogging risk, the least fresh water supply, the maximum water quality index improvement and the lowest bioremediation cost as constraint conditions, and inputting each ecological purification scheme into the constructed environmental fluid dynamic model one by one to obtain an optimal ecological purification scheme. A control strategy corresponding to the optimal ecological purification scheme is executed, and the control strategy at least comprises an ecological restoration strategy and a water conservancy control strategy. According to the method, the water environment quality of the target culture area can be remarkably improved, the waterlogging risk is reduced, and recovery and balance of an ecological system are promoted.

The invention belongs to the technical field of environmental protection of bioremediation wastewater, and discloses an immobilized adsorption microsphere, which is prepared from Bacillus spp., Candida utilis and European nitrosomonas through an immobilized embedding technology. The microspheres provided by the invention can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The invention discloses a method for regenerating and preparing soil for ecological restoration by utilizing microbial restoration pollution muck, which comprises the following steps: stirring and mixing dry straw powder and bimetallic alkali liquor, drying, heating to 500 DEG C or above under a protective atmosphere, and pyrolyzing to obtain a modified biochar material; mixing the modified biochar material with a microbial liquid, oscillating and adsorbing, and freeze-drying to obtain the carbon-based biological composite material. The carbon-based biological composite material and shield muck are mixed to obtain improved shield muck; mixing the improved shield muck with an organic fertilizer to obtain soil for ecological restoration; toxic and harmful components in the shield muck are removed through biodegradation, the shield muck is subjected to structure improvement through the modified biochar, then the composite fertilizer with specific components is matched, resource utilization of the shield muck is achieved, and the shield muck-based ecological restoration soil is obtained.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

An application and a composition of bioremediation agents and cremated remains forming underwater sanctuaries and structures by coral reefs comprising a homogenous mixture of calcium compounds, clay, cement, earthworm enzymes, and human / animal ashes. The composition will benefit nearby reefs with added calcium for rehabilitating reefs and earthworm enzymes with detoxifying properties. The underwater sanctuaries and structures will provide habitats for wildlife.

The invention discloses aquatic microbe and an application of the aquatic microbe in degradation of atrazine. The water microbe is named as Aquammicrobium lusiense HR8, and the preservation number of the water microbe is CGMCC (China General Microbiological Culture Collection Center) No. 28916. The invention also discloses a microbial inoculum containing the aquatic microbe, a method for culturing the aquatic microbe and a method for degrading triazine herbicides. The invention discloses that Aquammicrobe is used for degrading atrazine for the first time, the degradation rate of Aquammicrobe HR8 on 200 mg / L atrazine in 48 hours is 100%, the Aquammicrobe HR8 is applied to bioremediation treatment of an atrazine polluted environment, the defect that the bioremediation removal effect on atrazine is poor at the present stage can be overcome, and meanwhile secondary pollution to the environment is reduced.

The invention discloses a Gordonia ovorans YDY-4 strain and an application of the Gordonia ovorans YDY-4 strain in degradation of n-hexadecane. The cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 20251290, and the cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4 and is preserved in the China Center for Type Culture Collection (CCTCC). The Gordonia ovorans YDY-4 provided by the invention has the capability of degrading n-hexadecane under the condition that the pH (Potential of Hydrogen) is 7.0 to 9.0, and can grow at 28 to 32 DEG C. The Gordonia ovorans YDY-4 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a basis for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The invention belongs to the technical field of soil heavy metal pollution remediation, and discloses a heavy metal pollution soil bioremediation method and system based on soil metagenome sequencing, and the method comprises the following steps: carrying out gene sequencing on experimental microorganism class groups in heavy metal pollution soil to determine functional microorganisms containing heavy metal resistance genes; heavy metal resistant microorganism species in the soil can be accurately identified by judging whether functional genes are contained or not, and ecological unbalance possibly caused by blind introduction of alien species is avoided; a remediation strategy taking a microbial resistance mechanism as a core can effectively reduce the bioavailability of heavy metals in the soil, and also can improve the physicochemical properties of the soil through metabolic activity to form a sustainable remediation effect; the method has the advantages of being environmentally friendly, controllable in cost, lasting in effect and the like, and an innovative path is provided for soil ecological function restoration.

The invention belongs to the technical field of microorganisms, and particularly discloses a fluoroquinolone antibiotic efficient degrading bacterium and application thereof. The fluoroquinolone antibiotic degrading bacterium is Kocuria sp. X03, the preservation number of the Kocuria sp. X03 is CCTCC NO: M 2025522, the invention provides the fluoroquinolone antibiotic degrading bacterium Kocuria sp. X03 separated from soil, the Kocuria sp. X03 can efficiently degrade fluoroquinolone antibiotics, an antibiotic degrading strain library is enriched, biotransformation mechanism research and bioremediation application are promoted, and the application prospect is wide. The strain can be used for degrading and eliminating livestock and poultry manure and residual antibiotics in farmland soil, and has certain research and application values.

The invention discloses a desertification region soil ecology improvement and restoration system which comprises a multifunctional biochar preparation module used for collecting desertification region crop waste and preparing biochar through high-temperature pyrolysis to serve as a soil conditioner; the microbial remediation module is used for selecting drought-enduring plant species and culturing soil microorganisms; the resource comprehensive utilization module is used for collecting rainwater and establishing a water storage system; and the soil remediation device comprises a fertilization unit, an irrigation unit, a soil softening unit and a vegetation planting unit. Soil improvement is achieved through fertilization, irrigation and soil softening, the survival rate of later plants is increased, power is supplied to the soil remediation device in cooperation with the comprehensive resource utilization module, the utilization rate of renewable resources is increased, and therefore the ecological relation of mutual promotion among soil, vegetation and microorganisms is constructed; the resource comprehensive utilization module and the soil remediation device can guarantee sustainable development of the ecological relationship, and soil ecology improvement and remediation in the desertification area are achieved.

The invention discloses rahnella and application thereof, and belongs to the technical field of microbiology and biology, the rahnella S5-11 strain provided by the invention is a new species of rahnella, and the preservation number of the rahnella is GDMCC No: 63732. The strain S5-11 has relatively strong tolerance to selenate and selenite, the selenite and the selenite can be reduced into nano elemental selenium, a method for preparing a large amount of nano selenium by utilizing a bioreactor is established, and the strain S5-11 can be used for production of nano selenium and bioremediation of selenium-polluted environment and has a wide application prospect. Besides, the strain has good plant growth promoting ability, can promote crop growth in low-selenium soil, and can relieve stress of selenium on plants in high-selenium soil.

The invention discloses a planting method for overcoming continuous cropping obstacles of silage corn, which solves the problems of soil degradation and diseases and insect pests caused by continuous cropping by integrating soil physical remediation, chemical remediation, biological remediation, microbial community regulation and ecological intercropping technologies. The core technology content comprises the steps that deep ploughing is combined with organic matter and a biochar modifier to repair the soil structure, a compound microbial agent is sprayed to inhibit pathogenic bacteria, leguminous green manure and commercial crop rape are used for improving nitrogen circulation and reducing the autotoxicity effect every two years, and a precise fertilization management technology is matched. After the scheme is implemented, the soil health condition is remarkably improved, the silage corn yield is remarkably increased, and good ecological and economic benefits are achieved.

The invention discloses a Pseudomonas nicosulfurones bacterial strain and an application of the Pseudomonas nicosulfurones bacterial strain in degradation of phenanthrene and benzopyrene. According to the invention, a degradation strain Pseudomonas nicosulfurensis DY-8, which takes phenanthrene and benzopyrene as carbon sources, is obtained by domestication, separation and identification from petroleum-polluted soil in Ningbo. The invention also relates to a method for preparing the degradation strain Pseudomonas nicosulfurensis DY-8 with phenanthrene and benzopyrene as carbon sources. The DY-8 can utilize phenanthrene and benzopyrene as carbon sources, and after the DY-8 is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 70% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

The invention relates to the technical field of functional microbial remediation, in particular to Exiguobacterium basophilus and application thereof to purification of algal bloom saline-alkaline aquaculture water. The Exiguobacterium basophilus SADOM03 is preserved in the China General Microbiological Culture Collection Center on November 4, 2024, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.32489, and the Exiguobacterium basophilus SADOM03 is classified and named as Exiguobacterium basophilus. The Exiguobacterium basophilus SADOM03 provided by the invention can effectively decompose algae source dissolved organic matters in a water bloom saline-alkali aquaculture water body and remarkably remove organic pollutants and nitrogen and phosphorus nutritive salts in the water body, greatly improves the water quality, purifies the water body, and is expected to be applied to in-situ treatment of water bloom pollution of the saline-alkali aquaculture water body.

The invention discloses a method for biologically repairing soil by using crude oil degrading fungi, and belongs to the technical field of microorganisms and biology. The method comprises the following steps: step 1, directionally screening strains of the crude oil degrading fungi; step 2, function enhancement of the screened strains; 3, preparing a repair carrier; and 4, carrying out gradient process and composite flora synergistic remediation. According to the method, indigenous fungi are screened and separated in a crude oil pollution area, the screened strains are subjected to adaptive laboratory evolution, the degradation capacity and the environmental endurance capacity are enhanced, a preparation process of composite microspheres is combined, adsorption-degradation efficient remediation of crude oil pollution is achieved, and a gradient process and other biological methods are used for cooperating remediation measures; the remediation effect is further enhanced, the natural ecological environment is stably restored, secondary pollution in the remediation process is avoided, and the risk of ecological pollution is reduced.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

The invention relates to the field of environmental microbial remediation, in particular to a strain of trichlorobacterium and application thereof in bioremediation of pollutants. According to the present invention, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, and the bacillus subtilis is bacillus subtilis. The strain NB-12 obtained by the invention is a strain of self-synthesized cobalamin screened from river bottom sediments, is suitable for extensive industrial production and degrades a plurality of halogenated alkanes in the environment.