167 results about "Bioremediation" patented technology

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

The invention discloses a salicylic acid decarboxylase mutant and application of the salicylic acid decarboxylase mutant in degradation of salicylic acid. The mutant is obtained by carrying out site-specific modification on salicylic acid decarboxylase NahG, and specifically, valine at the 46th site, tyrosine at the 380th site and leucine at the 382nd site of an amino acid sequence shown in SEQ ID NO.2 are mutated into cysteine, phenylalanine and phenylalanine at the same time. The mutant shows remarkably improved catalytic efficiency, and salicylic acid can be efficiently and thoroughly decarboxylated and converted into catechol under mild conditions. The invention also provides a coding gene, a recombinant vector and an engineering bacterium of the mutant. The mutant not only can be used for efficient bioremediation of salicylic acid pollution, but also can effectively break through and accelerate a naphthalene catabolism pathway due to the characteristic of directionally generating catechol, and has important application value in the fields of environmental pollution abatement and biological catalysis.

The invention discloses an aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83 and application thereof, the strain is classified and named as Pseudomonas protegens, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.36443. The invention further discloses a preparation method of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The invention further discloses an application of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The strain has outstanding ammonia nitrogen metabolism capability under an aerobic condition, can quickly and efficiently convert ammonia nitrogen in a water body into a harmless or low-toxicity form, has wide tolerance and adaptability to environmental factors such as pH, temperature and the like, and is stable in performance. The strain is suitable for bioremediation of natural water, domestic sewage and various industrial wastewater, can effectively improve the denitrification efficiency and the system stability, and has the advantages of simplicity and convenience in operation, environmental friendliness and high practical application value.

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

This invention discloses an in-situ three-dimensional bioremediation method for slightly polluted water bodies. The method involves collecting a mud-water mixture from the water body to be treated, enriching and culturing it, and collecting the settled sludge. Culture medium II is added to the settled sludge for acclimatization and cultivation to prepare a biological agent. This biological agent is then added to a mixed solution of polyvinyl alcohol-hydrated calcium silicate-fulvic acid (PVA-CSH-FA) or PVA-modified zeolite-FA to prepare an immobilized biological carrier that can slowly release or adsorb calcium ions from the water. Calcium peroxide packing is also prepared to provide oxygen. The immobilized biological carrier packing and calcium peroxide packing are respectively fixed in the upper and lower layers of an ecological sedimentation bed, which is then placed in the slightly polluted water body to achieve in-situ three-dimensional bioremediation. The calcium-precipitating bacterial biological agent obtained through enrichment and acclimatization in this invention, when immobilized and mixed with the carrier material, efficiently and simultaneously removes pollutants such as nitrogen, phosphorus, organic matter, and heavy metals from slightly polluted water bodies.

The invention belongs to the technical field of heavy metal contaminated soil bioremediation, and particularly relates to a method for remediating heavy metal contaminated soil through a Fe3C-N-S composite modified electrode enhanced bioelectrochemical system. Graphene oxide, an iron source, a nitrogen source and a sulfur source serve as raw materials, a Fe3C-N-S composite material is prepared through high-temperature calcination, then the Fe3C-N-S composite material is compounded with sodium alginate (SA) hydrogel, the surface of a graphite rod is coated with the composite material, a Fe3C-N-S composite modified electrode is constructed through a CaCl2 cross-linking and freeze-drying technology, then the modified electrode is coupled with a graphite cathode, and the composite material is obtained. And the acidophilic thiobacillus ferrooxidans is combined to construct a bioelectrochemical system, so that the synergistic and efficient leaching of the heavy metals in the soil is realized. The conductivity, biocompatibility and corrosion resistance of the electrode can be remarkably improved, the problems that mass transfer is difficult and microorganism loading is unstable in BES remediation are solved, and a breakthrough technical scheme is provided for green and efficient remediation of heavy metal contaminated soil.

The present application relates to the field of microbial technology, and more particularly to a kind of for degrading high-efficiency fluazifop-butyl ester urea serratia DHPM01 and its application, urea serratia DHPM01 is preserved in China Microbial Culture Collection Center on September 18, 2025, preservation number is CGMCC NO.35971, and the address of preservation is Beijing Chaoyang District Beichen West Road No.1, and the classification designation is Serratia ureilytica The microbial agent provided by the present application can effectively degrade high-efficiency fluazifop-butyl ester in a wide pH and temperature range, and urea serratia DHPM01 can be used as an excellent pesticide-degrading bacterium and widely applied to the bioremediation of high-efficiency fluazifop-butyl ester residues in water, farmland and contaminated soil and other environments, providing a new efficient and environmentally friendly strategy for pesticide pollution control and enriching the germplasm resource bank of pesticide-degrading functional microorganisms.

A method for organic compound removal from a solution using microalgae includes extracting one or more hydrocarbons from an underground geologic formation to form a hydrocarbon composition and a produced water including at least a portion of the one or more hydrocarbons and one or more salts. The method further includes mixing the produced water with a wastewater to form a solution, and then contacting a microalgae feedstock, Chlorella sorokiniana, with the solution in a bioreactor. The total lipid content of the microalgae feedstock is 13 to 17%. The method includes removing the one or more hydrocarbons, nitrogen, and phosphorous from the solution by algal consumption with the microalgae feedstock. The method of the present disclosure removes 80 to 90 wt. % of the one or more hydrocarbons, 70 to 85 wt. % nitrogen, and 90 to 100 wt. % phosphorous.

The present application belongs to the field of microbial technology, and particularly relates to a new bacterial strain of Acinetobacter sp. and its use. The bacterial strain is isolated from mangrove soil, is a new species of Acinetobacter sp., is named as Acinetobacter sp. GXMZU3951, and the bacteria have been registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO: 36553. The Acinetobacter sp. GXMZU3951 of the present application has iron carrier production activity, can produce higher indole acetic acid, and has the ability to promote plant growth and the potential to resist heavy metals. The present application can be used in the biological remediation of heavy metal contaminated soil and as a green and environment-friendly biofertilizer for improving crop production, and has a good development and application prospect.

The invention relates to the technical field of environmental remediation microorganisms, and discloses a mixed polycyclic aromatic hydrocarbon degrading bacterium G3 suitable for variable environments and application thereof. The bacterial strain is pseudoxanthomonas sp. G3, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20251828. The strain G3 is enriched and separated from soil of a certain coking plant in Beijing, can utilize polycyclic aromatic hydrocarbons such as acenaphthene, fluorene, phenanthrene, fluoranthene and pyrene as a single carbon source, and can simultaneously degrade acenaphthene, fluorene, phenanthrene, fluoranthene and pyrene mixtures within 14 days, so that the degradation rate of each component is not lower than 70%; good growth and degradation activity are maintained under the conditions that the temperature is 20-37 DEG C, the salinity is 0-4% NaCl and the pH value is 5.0-9.0, and the degradation rates can reach 100% and 84.15% respectively after the strain is cultured in a single carbon source culture solution with the initial concentration of phenanthrene and pyrene being 50 mg.L <-1 > for 7 days; after the polycyclic aromatic hydrocarbon aging polluted soil is cultured for 28 days, the mixed polycyclic aromatic hydrocarbon in the soil can be effectively removed. According to the invention, the technical bottlenecks of poor environmental adaptability and low mixed pollutant degradation efficiency of the existing degrading bacteria are solved. Therefore, the strain has good application potential in the aspect of bioremediation of the polycyclic aromatic hydrocarbon polluted environment.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The invention discloses a nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1) with a capability of degrading multiple pollutants and an application of the nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1, and belongs to the technical field of screening and application of functional microorganisms. The strain is derived from a chicken manure aerobic composting environment, the preservation number is GDMCC No: 67351, and nitro polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons and tetracycline antibiotics can be efficiently degraded at the same time. In a single pollutant with the concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceed 97% in 8 h, the degradation rate of nitrofluoranthene reaches 99.01% in 48 h, and the degradation rate of phenanthrene reaches 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the 48-hour degradation rate is kept to be 78% or above. The strain is high in degradation efficiency and strong in environmental adaptability, provides a new microbial resource for bioremediation of a combined pollution site, and has a wide application prospect.

This invention discloses a composite nanofiber membrane for immobilizing microorganisms to remediate diesel-contaminated water and its preparation method. Specifically, the composite nanofiber membrane is a thermoplastic polyurethane / nanohydroxyapatite composite nanofiber membrane, which not only possesses excellent biocompatibility and mechanical strength but is also non-toxic and harmless, offering considerable cost-effectiveness. Upon introduction into diesel-contaminated water, the composite nanofiber membrane first rapidly adsorbs diesel at the water / oil interface, preventing further diffusion and toxic effects on the aquatic environment. Subsequently, due to the additional adsorption sites provided by the nanoparticles on the membrane, a large number of free bacteria are adsorbed onto the membrane, enhancing the metabolic activity of bacterial cells and their resistance to the toxicity of stubborn compounds, while also strengthening the affinity between hydrophobic compounds and cells, thereby increasing the degradation rate of diesel hydrocarbons by microbial cells. This invention improves the remediation effect of diesel wastewater while expanding the application of composite nanofiber membranes in the field of water pollution treatment.

The application discloses a soil enterobacter capable of efficiently degrading polypropylene and application thereof. The strain is FJNUIM-S2, which is classified and named as soil enterobacter (Enterobacter agglomerans) Enterobacter soli ), is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is preserved on October 17, 2024, with a preservation number of CGMCC No. 32241 and a preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Xiliujiaobei, Chaoyang District, Beijing. The strain FJNUIM-S2 of the application can grow with polypropylene as the only carbon source, has good polypropylene degradation characteristics, provides a microbial resource for bioremediation of waste polypropylene in the environment, and has a wide application prospect.

This invention discloses a benzo[a]pyrene-degrading fungus, *Nigrospora osmanthi* DL-7, for use in petroleum-contaminated soil, along with its inoculum and applications. This invention domesticated, isolated, and identified a benzo[a]pyrene-degrading strain, *Nigrospora osmanthi* DL-7, from petroleum-contaminated soil in Ningbo. DL-7 can utilize benzo[a]pyrene as a carbon source, even at an initial benzo[a]pyrene concentration of 25 mg·L⁻¹. ‑1 After culturing in an inorganic salt broth for 7 days, the pure DL-7 strain achieved a degradation rate of over 85% for benzo[a]pyrene. When prepared as a bacterial agent, the degradation rate of benzo[a]pyrene could be increased to over 90%. Therefore, this strain and its bacterial agent have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The present application belongs to the technical field of biological remediation and solid organic waste treatment and resource, and particularly relates to application of phenolic compounds directly degradable by laccase in compost and composting method for promoting humification. The present application uses phenolic compounds directly degradable by laccase in compost, and the composting process incubates a large number of microbial communities. When nutrients are sufficient, most of the microorganisms will show competitive response; and under stress environment, the microorganisms tend to be synergistic. In the early stage of the composting reaction, the humification process in the compost is often slow, and the microorganisms will make full use of the humic acid precursor, slowing down the composting. The toxicity of the phenolic compounds directly degradable by laccase can promote the synergistic function of the microorganisms in the compost, and because it can enhance the expression of laccase, it can promote the co-metabolic effect of laccase on pollutants and lignin, degrade the pollutants while promoting the humification of the compost, and protect the environment.

The invention relates to the technical field of environmental sludge treatment, in particular to sediment environment in-situ remediation particles and a preparation method thereof. The soil conditioner is prepared from the following raw materials: zeolite powder, volcanic rock powder, bentonite, silica sol, a functional component loaded precursor and bacillus spore powder. The sediment in-situ remediation particles are of a core-shell structure, activated zeolite-volcanic rock serves as an inner core to construct a porous framework, Fe / Mn and Mg / Ca loading sites are combined, pollutant adsorption, fixation and passivation are achieved, the sediment microenvironment is improved, conditions are created for outer-layer bacillus bioremediation, and space-time synergy of physicochemical and bioremediation is formed; a preparation route of curing nucleation and then low-temperature coating is adopted, the contradiction between mineral roasting and microorganism temperature intolerance is solved, the spore activity is guaranteed, and the loss resistance of the spore is improved.

The present disclosure relates to composition and methods of treating waste for the purposes of, including but not limited to, degrading waste, bioremediation of waste, reducing odor in waste, reducing organics in waste, and combinations thereof. More particularly, the present disclosure relates to the use of isolated Bacillus strains Bp D10, Bs E12, and strains having all of the identifying characteristics of those strains, and combinations thereof, for treating waste.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.

The invention discloses an industrial application device and method for microbe enhanced ex-situ remediation of petroleum-contaminated soil by applying ozone sequencing batch driving. According to the method, ozone and air with a certain concentration are intermittently and circularly injected into the soil containing the petroleum pollutants in an ectopic mode for a specific time period, and the contaminated soil is remedied through ozone oxidation coupled with microorganisms. According to the invention, ozone is injected to decompose organic pollutants which are difficult to degrade in petroleum into short-chain organic matters with good biodegradability by utilizing relatively strong oxidability and penetrability of ozone, so that the bioavailability of the pollutants is improved; air injection promotes microorganisms to decompose pollutants difficult to degrade in the soil, and alternate synergy of chemical oxidation and bioremediation is achieved through a sequencing batch type gas circulation injection mode, so that the overall remediation efficiency is improved, and then ectopic harmless treatment of the contaminated soil is achieved.

This invention discloses a strain of *Pseudomonas nicosulfuron-methyl* capable of degrading multiple pollutants. Pseudomonas nicosulfuronedens HD1 and its applications belong to the field of functional microbial screening and application technology. This strain, originating from an aerobic composting environment of chicken manure (accession number GDMCC No:67351), can simultaneously and efficiently degrade nitropolycyclic aromatic hydrocarbons (NPH), polycyclic aromatic hydrocarbons (PAHs), and tetracycline antibiotics. In single pollutants at a concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceeded 97% after 8 hours; the degradation rate of nitrofluoranthene reached 99.01% after 48 hours; and the degradation rate of phenanthrene reached 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the degradation rate remained above 78% after 48 hours. This strain exhibits high degradation efficiency and strong environmental adaptability, providing a new microbial resource for the bioremediation of multi-contaminated sites and possessing broad application prospects.

The invention provides a soil bioremediation process simulation parameter optimization method and system, and relates to the technical field of environmental engineering.The method comprises the steps that spatial distribution data of a target remediation area is obtained, the spatial distribution data is converted into a discrete sampling point set, and a virtual datum plane is constructed based on the discrete sampling point set; setting a reference area on the virtual reference plane, processing the discrete sampling point set, identifying clusters with relatively high spatial distribution density in the sampling point set, obtaining a minimum enclosing rectangle of the corresponding cluster by taking a boundary extreme point of each cluster as a basis, and dividing the reference area by taking the minimum enclosing rectangle as an initial structure partition, and obtaining a plurality of structure partitions. Accurate optimization of simulation parameters is achieved, and efficient and reliable parameter support is provided for soil bioremediation engineering.

This invention discloses a method for transporting *Pterygodon spp.* for benthic bioremediation, including pre-transportation pretreatment, low-temperature transportation, and post-transportation adaptation and domestication. Before transportation, by withholding feed and stimulating the expulsion of mud and sand by low osmotic pressure, the *Pterygodon spp.* effectively induces the *Pterygodon spp.* to contract its body segments, actively expel mud and sand to clean its intestines, and achieve intestinal and body surface purification, avoiding mechanical damage and water quality deterioration risks caused by mud and sand residue in the body during transportation. After transportation, adaptation and domestication can significantly reduce the *Pterygodon spp.*'s transportation stress response, reduce body segment damage caused by violent twisting, and induce the *Pterygodon spp.* to burrow naturally by creating a suitable substrate environment and tidal environment, quickly restoring its burrowing and benthic lifestyle.

The invention relates to the technical field of microbiology and selenium polluted environment restoration, and provides an ensifer morelensis R2-120 strain and an application thereof. The bacterial strain R2-120 is ensifer morelensis capable of efficiently synthesizing volatile selenium, and the preservation number of the bacterial strain R2-120 is GDMCC No: 63730. The strain R2-120 has relatively strong tolerance to selenate and selenite, and a plate test shows that the R2-120 can tolerate at least 200 mM of selenate and 60 mM of selenite. The strain R2-120 can metabolize selenite and convert the selenite into nano-selenium, and can convert the selenite and the nano-selenium into volatile selenium. The invention also establishes a method for removing selenium in the water body by using R2-120 in the bioreactor, and the method can be used for bioremediation of selenium-polluted water body and soil, treatment of selenium-containing wastewater and recycling of selenium, and has a wide application prospect.

A strain of antibiotic-resistant marine heterotrophic ammonia assimilation-aerobic denitrifying bacteria and its application, belongs to the field of water treatment of aquaculture. The seawater antibiotic-resistant high-efficiency nitrogen removal Marinobacter nitratireducens LAN01 can use polyhydroxyalkanoate as the sole carbon source, and can realize heterotrophic ammonia assimilation and aerobic denitrification function under the stress of different concentrations of sulfamethoxazole antibiotics, and simultaneously transform phosphate. The strain has multiple resistances such as high salinity, high alkalinity and antibiotic, and can be used for seawater aquaculture effluent treatment, seawater recirculating aquaculture water treatment, high-salinity wastewater treatment and saline-alkali soil bioremediation, etc. To realize the simultaneous recovery of nitrogen, phosphorus and other nutrients in the seawater aquaculture environment.

The invention discloses a microorganism colonization membrane assembly for river regulation, and relates to the technical field of river regulation, the microorganism colonization membrane assembly comprises a mounting box with an opening at the upper end, two partition plates are fixedly mounted in the mounting box at intervals, and the two partition plates sequentially divide the mounting box into a microorganism enrichment area, a metabolism diffusion area and a fluid exchange area from front to back; the three regions are respectively filled with a microorganism colonization membrane, a fluid flow guide membrane and a honeycomb membrane, and the side wall of one side, far away from the partition plates, of the microorganism enrichment region and the two partition plates are respectively provided with water permeable holes through which water flows to pass; the surface of the microbial colonization membrane is provided with nano-scale villus-shaped secondary ripples. According to the microorganism colonization membrane assembly for river regulation, through cooperation of the biological anchor points and the chemotactic factor coating, the microorganism attachment efficiency (the survival rate is increased by 40% or above) and the impact load resistance are remarkably improved, and the problems that in traditional biological remediation, microorganism colonization is difficult, and activity is low are solved.

This invention relates to the field of bioremediation technology for environmental pollutants, specifically to a method for degrading side-chain-less polycyclic aromatic hydrocarbons (PAHs) using the dual-oxygenation function of polycopper oxidases. This invention is the first to discover and demonstrate that polycopper oxidases, without the need for exogenous mediators, possess the ability to catalyze the dual-oxygenation reaction of side-chain-less PAHs, directly adding two oxygen atoms from molecular oxygen to the aromatic ring of PAHs to generate the corresponding dihydroxylated intermediate. Under further catalysis by polycopper oxidase, this intermediate selectively undergoes ring-opening cleavage at specific carbon-carbon bonds in the aromatic ring via a free radical mechanism, ultimately generating low-toxicity, readily bioavailable monobenzene ring or straight-chain ester compounds. The method provided by this invention is mild, environmentally friendly, and achieves thorough degradation, offering a highly efficient and green new technology for the bioremediation of PAH-contaminated soil, water bodies, and industrial waste.