1546 results about "Bioremediation" patented technology

The invention discloses a method for biologically remediating a water body and soil comprehensively utilizing resources. The method comprises the steps: (1), determining main pollutants in a selected land area or water body area; (2), selecting to plant and/or cultivate fast-growing herbaceous plants, fast-growing alga, trees, bushes, fungi or microorganisms with high remediation efficiency in the selected land area or water body area; (3), harvesting or collecting the fast-growing herbaceous plants and the fast-growing alga after growing to reach a suitable height or size; (4), concentratedly processing the harvested or collected fast-growing herbaceous plants and the fast-growing alga, and comprehensively utilizing to prevent the pollutants from dispersing; (5), remediating an eutrophic water body by adopting an artificial floating island; (6), remediating a heavy metal polluted water body by confining floating plants; (7), repairing cadmium and zinc polluted soil by mixed-cropping festuca arundinacea and bluegrass; (8), remediating polycyclic aromatic hydrocarbons (PAHs) polluted soil by mixed-cropping alfalfa and italian ryegrass; and (9), extracting and recovering silver from silver-containing wastewater remediation plants.

The invention includes a method and apparatus for composting organic material and maintaining composting conditions within preselected limits. Composting according to the method can occur within a sealable container. Adjusting the composition of the organic material to within preselected limits converts the organic material to a compostable mixture. The composition is adjusted by amending the organic material and by mixing the amended organic material with a bulking agent and an inoculant. Incubating forms a composting mixture from the compostable mixture. The method includes the steps of monitoring and adjusting conditions of the composting mixture to maintain the conditions within preselected limits. The conditions of the composting mixture are adjusted by aerating the composting mixture or remixing the composting mixture. The remixed composting mixture is incubated to yield composted organic material.

The invention discloses a bioremediation method for soil polluted by heavy metals. The bioremediation method comprises the following steps of: adding earthworm feeds into the soil polluted by the heavy metals, turning the soil, uniformly mixing, and putting earthworms into the soil and cultivating the earthworms; after the earthworms are cultivated for 28 to 35 days, collecting earthworm excrements in the upper layer soil, collecting adult earthworms by adopting a natural lighting method, and retaining young earthworms and earthworm eggs in the soil; planting plants by adopting a hedge planting method, cradling the parts of the plants growing for 78 to 90 days, which are positioned above the ground, and removing the whole plants after the plants grow for another 50 to 70 days; collecting the adult earthworms and the earthworm excrements for 1 to 3 days by adopting an outdoor collection method every 28 to 35 days during planting of the plants; and after the whole plants are removed, collecting all the adult earthworms and the young earthworms by adopting the natural lighting method, and collecting the earthworm excrements and the earthworm eggs. The soil is subjected to remediationby combining animals, plants and microorganisms according to a proper remediation sequence and a remediation period, so that the bioremediation method is economic, environment-friendly, easy to operate, high in remediation efficiency and easy to popularize, and is particularly suitable for mild or moderate copper, cadmium and lead combined polluted soils.

The invention discloses a new-typed salted soil modifier composition and making method, which comprises the following parts: superphosphate, calcium sulfate, poromeric material, decomposed rough organics, phosphogypsum, rough stream and macromolecular (water retention) agent for alkali soil of littoral area.

The invention provides a river surge silt d harmless and recycling treatment method. In the method, a dredger is utilized to suck silt, the silt is sent into a biochemical treatment pond from the ship through a silt transmission pipeline, compound enzyme is utilized to carry out bioremediation, gravels and sands with the particle size bigger than 1mm are filtered out by a primary hydrocyclone and a vibration screen, sands with the particle size of 0.075-1mm are filtered out by a secondary hydrocyclone and the vibration screen, powdery particles with the particle size of 0.025-0.075mm are filtered out by a third-level hydrocyclone and the vibration screen, powder particles are further separated out from outlet silt tail water through a sediment tower, sewage from the sediment tower is filtered by utilizing a fiber filter after flocculant is added into the sewage, and powdery particles are deposited from backflushing silt after being filtered by utilizing the sediment tower again, and the powdery particles are dehydrated by the third-level hydrocyclone and the vibration screen after entering a sludge pool and are cured by a curing agent after dehydration. The method has high resource utilization ratio, meets the requirements for the environmental protection, particularly carries out stage treatment on the silt, lowers the cost and enhances the efficiency.

The invention relates to an in-situ rain leaching repair system for contaminated soil. The in-situ rain leaching repair system at least comprises three parts, namely a rain collection part, a leaching repair part and a waste water treatment part, wherein the rain collection part is provided with water guiding pipes, a water collecting tank and canals, the leaching repair part is provided with an observation well, water collecting pipes and plants planted for the soil phytoremediation, the observation well is built in a middle-upper layer of contaminated soil, and the water collecting pipes are in the middle-lower layer; and the waste water treatment part is provided with a filter tank, a microorganism putting tank, an electric separation tank, a wind power generating set and the like, leaching waste water is firstly filtered by the filter tank, and is then repaired by the microorganism putting tank, and finally, the leaching waste water passes through the electric separation tank to process heavy metal so as to obtain the purified liquid. The in-situ rain leaching repair system establishes a complete contaminated soil simulated repairing system for the soil in an aeration zone, and combines repair technologies of leaching, plants, microorganisms, electric separation and the like. The in-situ rain leaching repair system has the characteristics of energy conservation, emission reduction and no secondary pollution, and is suitable for repairing the soil in a large-area aeration zone with little pollution.

This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010/mL, and that of Sphingobacterium multivorum is 107-1012/mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg/L, 1500 mg/L, 150 mg/L and 2500 mg/L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion/ml and the number of the effective viable bacteria in solid preparation reaches 1 billion/g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml/kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

A Rhodococcus ruber Em (CGMCC No.0868) features that its cells, cell suspension, or immobilized cells can degradate the paraffin and polycyclic arylhydrocarbon because the paraffin and polycyclic arylhydrocarbon are used as its only carbon source and energy source for growing to generate the bioemulsifier of ester. Said emulsifier can obviously decrease the surface tension of aqueous solution, has strong emulsifying power to ester substance, and can increase the solubility of paraffin and polycyclic arylhydrocarbon in water, so it can be used for treating the oil-contained sewage and repairing the petroleum polluted soil.

The invention discloses an in situ integrated restoring system and a method of petroleum polluted soil, and belongs to the technical field of petroleum polluted soil restoration. The system provided by the invention is composed of a suction well, a vacuum pump, a steam-water separation system, an oil-water separation system, a dosage pot, an injector pump, an injection well and an automatic control system. With the combination of the merits of vacuum suction and biological restoration, free-phase and high-density gas-phase pollutants in soil can be sucked and pumped in the invention; and simultaneously a surfactant reinforced biological restoration method can be adopted to accelerate the degradation or transformation and distribution of petroleum pollutants adsorbed on soil, so as to raise the in situ soil restoration efficiency, shorten the technological process, reduce the restoration cost and widen the application range of the in situ restoration technology.

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

Methods for enhancing bioremediation of ground water contaminated with nonaqueous halogenated solvents are disclosed. An illustrative method includes adding a composition to the ground water wherein the composition is an electron donor for microbe-mediated reductive dehalogenation of the halogenated solvents and enhances mass transfer of the halogenated solvents from residual source areas into the aqueous phase of the ground water. Illustrative compositions effective in these methods include surfactants such as C₂–C₄ carboxylic acids and hydroxy acids, salts thereof, esters of C₂–C₄ carboxylic acids and hydroxy acids, and mixtures thereof. Other illustrative compositions according to the present invention include oleyl lactylic acid and, optionally, oleic acid and lactic acid or salts thereof. Especially illustrative compositions for use in these methods include lactic acid, salts of lactic acid, such as sodium lactate, lactate esters, and mixtures thereof. The microbes are either indigenous to the ground water, or such microbes can be added to the ground water in addition to the composition.

The invention discloses a bioremediation method for petroleum-polluted soil. The method comprises the steps that 1, biomass containing biomass degrading bacteria is added in the petroleum-polluted soil with the petroleum content higher than 18 wt%, and ploughing is performed to mix the soil with the biomass, the water content of the petroleum-polluted soil is simultaneously kept at 10-30 wt%, and first degrading bacteria are added in the soil in 10-15 days after ploughing is performed to reduce the petroleum content of the soil to 8-18 wt%; 2, second degrading bacteria are added in the soil to reduce the petroleum content of the soil to 5-8 wt%; 3, third degrading bacteria are added in the soil to reduce the petroleum content of the soil to 1-5 wt%; 4, forth degrading bacteria are added in the soil to reduce the petroleum content of the soil below 1 wt%. The method can be applied to both in-situ remediation and ex-situ remediation, the remediation effect is good, the remediation time is short, the method is simple, and the cost is low.

The invention discloses a biological remediation type water system oil stain cleaning agent capable of quickly and effectively cleaning oil stain and decomposing oil stain in water by using a microorganism, a preparation method thereof and aims to solve the problems of more consumption, more pollution and bad cleaning effect when a traditional cleaning agent product is used for cleaning the oil stain, in particular to the problem that after the oil stain enters washing wastewater, as the pollution only can be transferred and can not be degraded, the serious environment friendliness can be caused. Aiming at the defects, the invention researches a novel bioremediation type water system oil stain cleaning agent which simultaneously contains a plurality of surfactants and microorganism bacterium agents for degrading the oil stain. Compared with the existing traditional cleaning agent, the novel bioremediation type water system oil stain cleaning agent has the remarkable advantages of quickness and high efficiency in washing, energy conservation and consumption reduction, direct oil stain degradation, environment friendliness and emission reduction, and the like. The bioremediation type water system oil stain cleaning agent for quickly degrading the oil stain has the advantages of good stability, high safety, strong oil degradation activity, quick growth and no secondary pollution tn the environment.

The invention relates pseudomonas, use thereof and a method for removing cadmium pollution to the environment. The pseudomonas class is named pseudomonas putidaHN103 and the collection number in a collection center is CCTCCNo.M2011184; and the shape and characteristics of the pseudomonas putidaHN103 comprise: 1) on a culture medium, a bacterial colony which used to be colorless and transparent turns white, smooth and convex-like with irregular edges; and 2) according to the result of bacterial microscopic examination, pseudomonas belongs to brevibacterium and is gram negative. The use of the bacteria is the use of the bacteria as a biological repair material for removing cadmium pollution. The method for removing cadmium pollution to the environment comprises the following steps: 1) regulating the cadmium ion concentration in sewage to a range in which pseudomonas can grow normally; 2) placing pseudomonas; 3) keeping the pseudomonas in the sewage for a certain time period; and 4) removing bacterial absorbing cadmium ions. The pseudomonas requires small investment, is low in cost, high in adaptability and capable of effectively removing cadmium, can quickly and obviously lower the concentration of cadmium ions in the environment and is suitable for biological remediation environments polluted by cadmium.

The invention discloses an aquaculture water purifying agent. The aquaculture water purifying agent comprises the following components in part by weight: 5 to 20 parts of tourmaline, 20 to 60 parts of montmorillonite, 100 to 500 parts of zeolite, 5 to 30 parts of molasses, 0.5 to 5 parts of phosphate, 0.1 to 1 part of tryptophan and 10 to 50 parts of microbial agent. The purifying agent is compounded by using the microbial agent, mineral biological activating agents and nutrient elements; and the tourmaline, the montmorillonite and the zeolite can promote microbe growth and metabolic activity, and can be attached to or fix microbes to play a barrier protection role. The nutrient elements can regulate the carbon nitrogen ratio balance of the water and promote the growth and the degrading effect of the microbes, and the zeolite and the montmorillonite are good controlled release carriers and have remarkable controlled release effect on the nutrient elements. The purifying agent can strengthen and activate the metabolic activity and the environmental adaptability of the microbial agent; and by compounding the minerals and the nutrient elements, the optimal ecological environment of microbe degradation is regulated and controlled, a biodegradable micro-ecological system for constructing the culture water is optimized and biological repair of the water is promoted.