398 results about "Bioremediation" patented technology

The invention discloses an acid mine wasteland ecological restoration plant dynamic optimization configuration method, and relates to the field of ecological restoration, and the method comprises the following steps: 1, collecting soil attribute data in a predetermined range of an acid mine wasteland in a dotted manner, and synchronously obtaining growth attribute data of to-be-selected plants and periodic weather data of a target area; step 2, extracting a deterioration feature set having a deterioration effect on plant growth based on the soil attribute data, and analyzing a bioremediation feature set capable of inhibiting the deterioration feature in the plant attribute data to generate a candidate plant set; 3, constructing a dynamic environment simulation model based on a deep learning algorithm, wherein input dimensions comprise weather data time sequence features, soil deterioration feature vectors and a plant bioremediation feature matrix; the method can accurately predict the restoration effects of plants under different climate and soil conditions, simulate the effects of different intervention measures in real time, output the optimal intervention scheme, and ensure the balance between the resource utilization efficiency and the restoration effects.

The invention discloses ultra-high performance concrete and a preparation method thereof. The ultra-high performance concrete comprises the following components in percentage by mass: 15%-25% of cement, 3%-6% of nano SiO2, 30%-35% of fly ash or steel slag powder, 35%-40% of recycled aggregate, 1%-4% of fiber, 0.5% of a microbial remediation agent and 1%-3% of nano TiO2. The preparation method comprises the following steps: S1, pretreating the recycled aggregate; and S2, packaging the microbial remediation agent: mixing the bacillus pasteurii spores with calcium lactate, and wrapping the mixture in calcium phosphate microspheres. S3, step-by-step stirring; S3.1, dry mixing: uniformly stirring the cement, the fly ash, the nano SiO2, the nano TiO2 and the recycled aggregate; s3.2, wet mixing is carried out, water solution fibers containing the polycarboxylic acid water reducing agent are added, uniform stirring is carried out, and the water-binder ratio of the water solution containing the polycarboxylic acid water reducing agent is 0.18; s3.3, final mixing: adding the packaged microbial remediation agent, and uniformly stirring; and S4, directional curing: performing steam curing at 80 DEG C for 48 hours, performing ultraviolet irradiation, and performing constant-temperature and constant-humidity curing for 7 days. The ultra-high performance concrete has the effects of low cost, low carbon emission, multi-function integration and repeatable crack self-repairing.

The invention discloses a haloalkane dehalogenase mutant and an application thereof in degradation of 1, 2, 3-tribromopropane, haloalkane dehalogenase DhaA31 coded by dhaA gene of a Rhodococcus rhodococcus NCIMB 13064 strain is modified to obtain the haloalkane dehalogenase mutant F168W / I246C, the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane, and the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane under the condition of water phase normal temperature. The degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane within 1 h reaches 100%, and the degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane reaches 100%. The haloalkane dehalogenase mutant has high stereoselectivity on (R)-2, 3-dibromo-1-propanol, and the e.r. Value of the haloalkane dehalogenase mutant reaches 6: 94. The haloalkane dehalogenase mutant provided by the invention has high catalytic efficiency and high stereoselectivity, is beneficial to recognition and further degradation of downstream enzymes, and has a good application prospect in bioremediation of emerging haloalkane pollutants.

The microbial remediation ceramsite comprises microorganisms, nutrient substances, modified porous slow-release Ca < 2 + > ceramsite and a cement-based protective shell, the microorganisms and the nutrient substances are loaded in the modified porous slow-release Ca < 2 + > ceramsite, and the cement-based protective shell wraps the outer surface of the modified porous slow-release Ca < 2 + > ceramsite. The surface of the ceramsite is modified by an aggregation-crosslinking method, and the modified microbial ceramsite carrier has higher microbial loading capacity and better stability under the dual actions of physical adsorption and valence bond combination, so that the repair effect of the ceramsite on cracks is further ensured; the cement-based protective shell adopts epoxy resin, so that the cylinder compressive strength of the ceramsite is improved, and microorganisms and nutrient substances are protected from being damaged by a high-alkalinity environment; the cement-based mixed powder is selected to enhance the interface adhesive force between the ceramsite and cement concrete, the sulphoaluminate cement in the mixed powder ensures the strength of the ceramsite in the early stage, and the fly ash, the silica fume, the nano-silica and other materials enable the later strength of the ceramsite to be continuously increased.

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention discloses an aquaculture non-point source pollution treatment method based on ecological-hydraulic engineering, which comprises the following steps: acquiring water quality monitoring basic data of a target aquaculture area, inputting the water quality monitoring basic data into an ecological simulation environment matched with an ecological-hydraulic engineering fishpond, and generating a plurality of ecological purification schemes, constructing an environmental fluid dynamic model corresponding to the ecological-water conservancy project fishpond by taking the lowest waterlogging risk, the least fresh water supply, the maximum water quality index improvement and the lowest bioremediation cost as constraint conditions, and inputting each ecological purification scheme into the constructed environmental fluid dynamic model one by one to obtain an optimal ecological purification scheme. A control strategy corresponding to the optimal ecological purification scheme is executed, and the control strategy at least comprises an ecological restoration strategy and a water conservancy control strategy. According to the method, the water environment quality of the target culture area can be remarkably improved, the waterlogging risk is reduced, and recovery and balance of an ecological system are promoted.

The invention belongs to the technical field of environmental protection of bioremediation wastewater, and discloses an immobilized adsorption microsphere, which is prepared from Bacillus spp., Candida utilis and European nitrosomonas through an immobilized embedding technology. The microspheres provided by the invention can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

An application and a composition of bioremediation agents and cremated remains forming underwater sanctuaries and structures by coral reefs comprising a homogenous mixture of calcium compounds, clay, cement, earthworm enzymes, and human / animal ashes. The composition will benefit nearby reefs with added calcium for rehabilitating reefs and earthworm enzymes with detoxifying properties. The underwater sanctuaries and structures will provide habitats for wildlife.

The invention discloses a Gordonia ovorans YDY-4 strain and an application of the Gordonia ovorans YDY-4 strain in degradation of n-hexadecane. The cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 20251290, and the cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4 and is preserved in the China Center for Type Culture Collection (CCTCC). The Gordonia ovorans YDY-4 provided by the invention has the capability of degrading n-hexadecane under the condition that the pH (Potential of Hydrogen) is 7.0 to 9.0, and can grow at 28 to 32 DEG C. The Gordonia ovorans YDY-4 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a basis for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The invention belongs to the technical field of soil heavy metal pollution remediation, and discloses a heavy metal pollution soil bioremediation method and system based on soil metagenome sequencing, and the method comprises the following steps: carrying out gene sequencing on experimental microorganism class groups in heavy metal pollution soil to determine functional microorganisms containing heavy metal resistance genes; heavy metal resistant microorganism species in the soil can be accurately identified by judging whether functional genes are contained or not, and ecological unbalance possibly caused by blind introduction of alien species is avoided; a remediation strategy taking a microbial resistance mechanism as a core can effectively reduce the bioavailability of heavy metals in the soil, and also can improve the physicochemical properties of the soil through metabolic activity to form a sustainable remediation effect; the method has the advantages of being environmentally friendly, controllable in cost, lasting in effect and the like, and an innovative path is provided for soil ecological function restoration.

The invention belongs to the technical field of microorganisms, and particularly discloses a fluoroquinolone antibiotic efficient degrading bacterium and application thereof. The fluoroquinolone antibiotic degrading bacterium is Kocuria sp. X03, the preservation number of the Kocuria sp. X03 is CCTCC NO: M 2025522, the invention provides the fluoroquinolone antibiotic degrading bacterium Kocuria sp. X03 separated from soil, the Kocuria sp. X03 can efficiently degrade fluoroquinolone antibiotics, an antibiotic degrading strain library is enriched, biotransformation mechanism research and bioremediation application are promoted, and the application prospect is wide. The strain can be used for degrading and eliminating livestock and poultry manure and residual antibiotics in farmland soil, and has certain research and application values.

The invention discloses a planting method for overcoming continuous cropping obstacles of silage corn, which solves the problems of soil degradation and diseases and insect pests caused by continuous cropping by integrating soil physical remediation, chemical remediation, biological remediation, microbial community regulation and ecological intercropping technologies. The core technology content comprises the steps that deep ploughing is combined with organic matter and a biochar modifier to repair the soil structure, a compound microbial agent is sprayed to inhibit pathogenic bacteria, leguminous green manure and commercial crop rape are used for improving nitrogen circulation and reducing the autotoxicity effect every two years, and a precise fertilization management technology is matched. After the scheme is implemented, the soil health condition is remarkably improved, the silage corn yield is remarkably increased, and good ecological and economic benefits are achieved.

The invention discloses a Pseudomonas nicosulfurones bacterial strain and an application of the Pseudomonas nicosulfurones bacterial strain in degradation of phenanthrene and benzopyrene. According to the invention, a degradation strain Pseudomonas nicosulfurensis DY-8, which takes phenanthrene and benzopyrene as carbon sources, is obtained by domestication, separation and identification from petroleum-polluted soil in Ningbo. The invention also relates to a method for preparing the degradation strain Pseudomonas nicosulfurensis DY-8 with phenanthrene and benzopyrene as carbon sources. The DY-8 can utilize phenanthrene and benzopyrene as carbon sources, and after the DY-8 is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 70% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

The invention discloses a method for biologically repairing soil by using crude oil degrading fungi, and belongs to the technical field of microorganisms and biology. The method comprises the following steps: step 1, directionally screening strains of the crude oil degrading fungi; step 2, function enhancement of the screened strains; 3, preparing a repair carrier; and 4, carrying out gradient process and composite flora synergistic remediation. According to the method, indigenous fungi are screened and separated in a crude oil pollution area, the screened strains are subjected to adaptive laboratory evolution, the degradation capacity and the environmental endurance capacity are enhanced, a preparation process of composite microspheres is combined, adsorption-degradation efficient remediation of crude oil pollution is achieved, and a gradient process and other biological methods are used for cooperating remediation measures; the remediation effect is further enhanced, the natural ecological environment is stably restored, secondary pollution in the remediation process is avoided, and the risk of ecological pollution is reduced.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

The invention relates to the field of environmental microbial remediation, in particular to a strain of trichlorobacterium and application thereof in bioremediation of pollutants. According to the present invention, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, the bacillus subtilis is bacillus subtilis, and the bacillus subtilis is bacillus subtilis. The strain NB-12 obtained by the invention is a strain of self-synthesized cobalamin screened from river bottom sediments, is suitable for extensive industrial production and degrades a plurality of halogenated alkanes in the environment.

The invention discloses a bacterial strain for reducing dissimilatory nitrate into ammonia nitrogen through anaerobic biodegradation of polycyclic aromatic hydrocarbon and application of the bacterial strain, the bacterial strain is named as PheICNA, and the bacterial strain belongs to Intraporum calvum. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40686. The strain can degrade polycyclic aromatic hydrocarbons such as phenanthrene under anaerobic conditions and completely metabolize the polycyclic aromatic hydrocarbons into carbon dioxide. Meanwhile, nitrate radicals are reduced into ammonium nitrogen, and the generated ammonium nitrogen can be used for growth of microorganisms and can be used as a nitrogen source for plants. The strain can be used for biologically repairing polycyclic aromatic hydrocarbon pollutants such as phenanthrene in soil, can convert nitrate nitrogen in the soil into ammoniacal nitrogen at the same time, achieves the effect of nitrogen maintenance, and has good application prospects in the aspects of polluted soil treatment and the like.

The invention discloses geotrichum candidum and application thereof in degradation of a polyethylene plastic mulching film, and relates to the technical field of microorganisms and application thereof. The strain number of the Geotrichum candidum is MY-0603, the Geotrichum candidum is preserved in the China Center for Type Culture Collection, and the preservation number of the Geotrichum candidum is CCTCC (China Center for Type Culture Collection) NO: M2025455. Experimental results show that the screened strain shows excellent performance in the aspect of biodegradation of polyethylene plastic, and after 21 days of biodegradation treatment, the weight loss rate of the polyethylene plastic mulching film reaches 8%. The method has the advantages of being environmentally friendly, low in cost, easy and convenient to operate and the like, and the obtained strain has the efficient degradation characteristic. Therefore, the invention provides a new strain resource and thought for bioremediation of polyethylene waste in the environment, and has a wide application prospect.

The invention discloses a bacterium DY-11 for degrading phenanthrene and benzopyrene in petroleum-contaminated soil and application of the bacterium DY-11. The bacterial strain is Achromobacter expander DY-11, and the preservation number of the bacterial strain is GDMCC No: 66005. Experiments prove that the strain DY-11 can respectively utilize phenanthrene and benzopyrene as carbon sources to degrade phenanthrene and benzopyrene, and after the strain DY-11 is cultured in inorganic salt culture solutions with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 65% or above. Therefore, the DY-11 is a strain which can degrade phenanthrene and benzopyrene and has very strong tolerance to phenanthrene and benzopyrene, has strong adaptability to polycyclic aromatic hydrocarbons, and has better application potential in the aspect of bioremediation.

The present invention discloses Gordonia for degrading various phthalic acid esters and its application in repairing phthalic acid ester-polluted soil. A strain of Gordonia FV1 was isolated from contaminated soil, and its microbial preservation number is: CGMCC No. 30310. This strain still has excellent degradation ability for phthalic acid esters within the temperature range of 10-60 °C or within the pH value range of 5.0-11.0, and has strong environmental adaptability. The Gordonia FV1 can degrade 14 phthalic acid esters, covering short-chain PAEs, medium-chain PAEs, long-chain PAEs, and complex-chain PAEs. Applying the Gordonia FV1 to bioremediate soil can effectively remove bis(2-ethylhexyl) phthalate in the soil. The present invention has application prospects in aspects such as microbial degradation of phthalic acid esters and bioremediation of soil.

The invention provides bacillus mycoides Y2 with functions of reducing cadmium content of crops and promoting growth and application of the bacillus mycoides Y2, and relates to the technical field of soil heavy metal pollution bioremediation and agricultural microorganism application. The bacillus mycoides Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2025, and the preservation number is CGMCC 35231. The strain can be applied to cadmium-polluted soil through root application, can reduce the cadmium content of edible parts of crops such as rice, oilseed rape and fast vegetables, and promotes crop growth and rice root surface iron plaque formation. The contaminated soil in Hunan, Henan and Tianjin is verified to be capable of stably colonizing and adapting to different soils and crops. The strain and the matched preparation are green, efficient, high in stability and wide in universality, and provide key technical support for safe utilization of cadmium-polluted cultivated land.

The invention discloses a salicylic acid decarboxylase mutant and application of the salicylic acid decarboxylase mutant in degradation of salicylic acid. The mutant is obtained by carrying out site-specific modification on salicylic acid decarboxylase NahG, and specifically, valine at the 46th site, tyrosine at the 380th site and leucine at the 382nd site of an amino acid sequence shown in SEQ ID NO.2 are mutated into cysteine, phenylalanine and phenylalanine at the same time. The mutant shows remarkably improved catalytic efficiency, and salicylic acid can be efficiently and thoroughly decarboxylated and converted into catechol under mild conditions. The invention also provides a coding gene, a recombinant vector and an engineering bacterium of the mutant. The mutant not only can be used for efficient bioremediation of salicylic acid pollution, but also can effectively break through and accelerate a naphthalene catabolism pathway due to the characteristic of directionally generating catechol, and has important application value in the fields of environmental pollution abatement and biological catalysis.

The invention belongs to the technical field of environmental protection, and relates to screening, domestication and application of focused polyacrylamide (PAM) for efficiently degrading fungi. The strain (# 12) is preserved in CGMCC (China General Microbiological Culture Collection Center) and is trichoderma asperellum, and the similarity between the ITS sequence of the strain and the gene reaches 99%. Aiming at soil hardening and acrylamide pollution caused by difficult degradation of non-ionic PAM in bauxite slime, the inventor adopts a gradient concentration screening and multi-stage domestication process. The method comprises the following steps: carrying out enrichment culture on slurry containing PAM, carrying out gradient screening in a basic solution with a specific concentration, separating and purifying to obtain an optimal strain, and carrying out continuous passage domestication through a six-stage culture medium, so that the degradation rate is increased by 23.45%. Under optimized conditions, the PAM degradation rate of the strain reaches 64.18%, the viscosity is reduced by 88.03%, degradation is achieved through physical destruction and enzymatic hydrolysis, and no acrylamide monomer is detected. The strain has activity at the temperature of 25-45 DEG C and the pH value of 4-8, is suitable for bioremediation of PAM-containing slime, alleviation of soil hardening and elimination of risks of toxic and side products, reveals the efficient degradation characteristic of trichoderma fungi to nonionic PAM for the first time, and assists in recycling of mining wastes.

The invention relates to the technical field of soil remediation, in particular to an efficient in-situ microbial remediation method for organic contaminated soil, which comprises the following steps: layering and dividing the contaminated soil into layers and regions, and injecting different medicaments into circulating well groups positioned in different regions to achieve the purpose of adjusting an underground microbial environment, and automatically forming and constructing an aerobic-anaerobic transition zone. Meanwhile, self-circulation of underground water is accelerated through the circulating well group, different indigenous microorganism florae are activated, and a metabolic chain is formed; and aerobic / anaerobic microorganisms are periodically injected into the injection well, an electron donor and a carbon source are synchronously provided, the steady state of an ecosphere is maintained, and the aims of optimizing the construction period and the treatment effect of the in-situ bioremediation technology of the polluted soil and the underground water are achieved.

The invention discloses an aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83 and application thereof, the strain is classified and named as Pseudomonas protegens, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.36443. The invention further discloses a preparation method of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The invention further discloses an application of the aerobic high-efficiency ammonia nitrogen degradation strain TYF-QDKJ-P83. The strain has outstanding ammonia nitrogen metabolism capability under an aerobic condition, can quickly and efficiently convert ammonia nitrogen in a water body into a harmless or low-toxicity form, has wide tolerance and adaptability to environmental factors such as pH, temperature and the like, and is stable in performance. The strain is suitable for bioremediation of natural water, domestic sewage and various industrial wastewater, can effectively improve the denitrification efficiency and the system stability, and has the advantages of simplicity and convenience in operation, environmental friendliness and high practical application value.

The present invention relates to the technical field of environmental pollution remediation, and specifically discloses a bioremediation method for degrading chlorinated hydrocarbons using microorganisms in collaboration with colloidal coconut shell biochar, and its application. The method comprises: preparing a combined bacterial agent, preparing colloidal coconut shell biochar powder, constructing a collaborative degradation system and designing a reaction system, and on-site in-situ remediation application. The method effectively overcomes the problems of insufficient microbial activity, unstable electron donor supply, and saturation of adsorbent materials in traditional bioremediation. The present invention can always maintain the remediation process in an optimal state, effectively improving the engineering practicality and reliability of on-site remediation. The method is simple to operate, has low energy consumption, and is cost-effective. It also has high environmental adaptability and long-term remediation effects, conforming to the concept of green remediation. The technical solution of the present invention is not only suitable for ex-situ remediation verification, but can also be extended to actual engineering applications in complex contaminated sites, with broad market prospects and promotional value.

The invention discloses bacillus cereus with wide pH adaptability and application of the bacillus cereus in soil or underground water heavy metal remediation, the strain is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20241939), and the bacillus cereus is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (CCTCC). The strain is high in environmental adaptability and high in remediation efficiency, can show good reduction and endurance capacity to various heavy metal pollutants under the wide environmental conditions that the pH ranges from 4 to 10 and the temperature ranges from 16 DEG C to 37 DEG C, and is particularly suitable for in-situ remediation of high-concentration contaminated soil; experimental results represented by hexavalent chromium show that the removal rate of the strain in 1000 mg / kg hexavalent chromium contaminated soil within 7 days can reach 70-100%, the dependence of an existing microbial remediation technology on a low-concentration and alkaline environment or a long remediation period is broken through, and an efficient solution is provided for green bioremediation of multiple types of heavy metal contaminated sites.