33 results about "Autotoxicity" patented technology

The invention discloses a hot pepper pepper continuous cropping autotoxicity concave soil matrix restorative agent, a preparation method thereof and a seedling matrix preparation method thereof. The concave soil restorative agent comprises the following components by mass percent: 40-65% of concave soil, 25-40% of waste coal cinders, 2-8% of active carbon, 0.8-1.5% of hydrated lime and 0.5-2% of humic acid. According to the invention, the concave soil restorative agent can solve the problem that autotoxicity caused by autotoxins is difficult to eliminate by adopting traditional agronomic measures such as rotating intercropping, soil improvement and ectopic seedling, so that the formation and aggravation of continuous cropping obstacles can be effectively relieved.

The invention provides a soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, a preparation method and application of the soil remediation organic fertilizer. The soil remediation organic fertilizer is prepared by conducting mixed fermentation on crop straw, a straw decomposition agent and N, P and K fertilizer. The straw decomposition agent is obtained by conducting crossing antagonistic culture on four strains, wherein bacillus subtilis is used for inhibiting and killing harmful bacteria and pathogenic bacteria in soil, trichoderma reesei is used for decomposing straw fibers and quickly decomposing the straw, lactobacillus plantarum is used for inhibiting and killing pests and pest eggs in the soil, and bacillus mucilaginosus is used for removing autotoxicity residues of panax notoginseng. The microbial agent cooperates with the crop straw and the N, P and K fertilizer to achieve the effects of overcoming continuous cropping obstruction inducements such as soil-borne pathogen accumulation, soil nutrient unbalance and plant autotoxicity, supplementing soil organic matter and optimizing the aggregate structure of the soil, therefore, water retention, water drainage and ventilation are achieved, and crops are developed in root system and healthy and strong in plant.

The invention relates to additive for relieving chilli autotoxicity and preparation method thereof. The additive comprises the following components in percentage by weight: 47.6 percent of pulverized maize straw, 4.8 percent of horse manure and 47.6 percent of water. The preparation method comprises the following steps of: (1) weighing 47.6 weight percent of the pulverized maize straw and uniformly mixing the pulverized maize straws with 47.6 weight percent of the water and 4.8 weight percent of the horse manure so as to ensure that the water soaks the maize straws which is in sufficient contact with the horse manure; (2) putting the uniformly mixed mixture of the maize straws, the horse manure and the water in a plastic-covered tunnel with a day temperature of 25-30 DEG C and a night temperature of 20-25 DEG C to decompose for 50-150 days; during decomposing, turning out the mixture every 5 days and complementing water, wherein during decomposing, the water content is 50-65 percent; and (3) obtaining a decomposed product namely the additive for relieving the chilli autotoxicity. The invention can replace crop rotation and overcome chilli continuous cropping obstacles, is simple and convenient to apply and suitable for a modern vegetable production mode and can avoid pollutions to the environment.

The invention discloses a peanut successive cropping obstacle conditioner based on the allelopathic autotoxicity effect and an application method thereof. The conditioner is composed of, by mass, 3%-10% of phenylalanine, 0.2%-1% of o-tert-butyl phenol, 0.2%-1% of 3,5-dimethylbenzoic acid, 0.2%-1% of isoeugenol, 0.5%-3% of salicylic acid, 0.3%-1% of borax, 0.3%-1% of sodium molybdate, 0.5%-3% of zinc sulfate, 0.5%-3% of ferrous sulfate, 0.5%-3% of manganese sulfate, 3%-10% of ethyl alcohol and 65%-90% of water, wherein the mass percent sum of all the components is 100%. The conditioner is simple in preparation technology and low in cost. The self-poison resistance of peanut plants is improved; meanwhile, growth is conditioned, the stress resistance is enhanced, the peanut successive cropping obstacle solving effect is good, and the yield is increased by 15%-20% compared with a control group to which the conditioner is not applied.

The present invention provides a soil remediation organic fertilizer for overcoming a watermelon replantation obstacle, a preparation method thereof and an application thereof. Agricultural straws, a straw-decomposing inoculant, an N fertilizer, a P fertilizer and a K fertilizer are subjected to mixed fermentation to prepare the soil remediation organic fertilizer. The straw-decomposing inoculant is prepared through subjecting four strains to cross antagonism and cultivation. The above four strains are composed of bacillus subtilis, Trichodermareesei, lactobacillus plantarum and Lactobacillus acidophilus. The bacillus subtilis is used for inhibiting and killing harmful bacteria and pathogenic bacteria in the soil. The Trichodermareesei is used for decomposing straw fibers and quickly composting straws. The lactobacillus plantarum is used for inhibiting and killing insects and worm eggs in the soil. The Lactobacillus acidophilus is used for removing the autotoxicity residues of watermelons. The effects of fungicides are realized based on the mutual coordination of the fungicides, agricultural straws, N, P and K fertilizers. Therefore, the accumulation of soil-borne pathogens, the soil nutrient imbalance and the plant autointoxication, which are inducement for replantation and successive cropping obstacles, can be avoided. Meanwhile, soil organic matters are supplemented, and the soil aggregate structure is optimized. Therefore, the soil remediation organic fertilizer has the advantages of water retention, drainage and ventilation, so that the plant root system is developed. Plants are enabled to grow healthily.

According to shade tolerance of Lanzhou lily which is a perennial plant, wide and narrow belt alternated planting is adopted for interplanting, each wide lily belt is interplanted with a row of corns or two rows of broad beans, an interplanting period is three years, lilies are interplanted with the corns in the first year, and then the broad beans take the succession of corn belts in the next year. By the corns and the broad beans for crop rotation, a space barrier can be formed to inhibit large-area spreading of lily leaf wilt and root system diseases, and the plant infection rate is decreased by more than 60% as compared with that of single planting of the lilies; disorders of root exudate autotoxicity due to perennation of the lilies are abated, a community structure of soil microorganisms is changed, and development of pathogenic microorganisms is inhibited.

The invention discloses a microbial agent for inhibiting tobacco continuous cropping obstacles. The microbial agent is prepared by uniformly mixing a nitrogen-fixing bacterium agent, a potassium-dissolving bacterium agent, a phosphorus-dissolving bacterium agent and a benzoic acid degrading bacterium agent according to a ratio of 1:1:1:1, wherein the nitrogen-fixing bacterium agent is a powdery inoculant prepared by culturing azotobacter chroococcum strains, the potassium-dissolving bacterium agent is a powdery inoculant prepared by culturing a bacillus mucilaginosus strain, the phosphorus-dissolving bacterium agent is a powdery mixed inoculant prepared by culturing a pseudomonas strain, and the benzoic acid degrading bacteria bacterium agent is a powdery inoculant prepared by culturing anaspergillus flavus strain. The microbial agent is a composite microbial agent, can quickly recover the problem of soil bacterial colony imbalance caused by tobacco continuous cropping, can improve the allelopathy and autotoxicity of tobacco continuous cropping root exudates on tobacco, and has a better effect of overcoming tobacco continuous cropping obstacles.

The invention provides a strain of Bacillus subtilis, a screening method and applications thereof, wherein the Bacillus subtilis is preserved in the China Center for Type Culture Collection, has the preservation number of CCTCC No:M 2017729, has the classification name of Bacillus subtilis L501, is mainly used for the degradation of strawberry succession cropping obstacle induced autotoxicity substance benzoic acid, wherein the benzoic acid degradation rate is up to 87.5%. According to the present invention, with the Bacillus subtilis, benzoic acid in the rhizosphere of strawberry is effectively degraded, the main strawberry succession cropping obstacle is overcome, and the technical method is provided for the effective utilization of bacteria to treat strawberry succession cropping obstacle in strawberry planting production in the future so as to achieve the large-scale production of strawberry, increase the yield of strawberry, provide the technical support for the effective use of bacteria to treat strawberry succession cropping obstacle in strawberry planting production, and provide broad market prospect.

The invention relates to bio-organic fertilizer for preventing tomato autotoxicity. The bio-organic fertilizer is mainly prepared by fermenting, by mass, 45-55% of coal humic acid, 20-35% of corn meal, 5-10% of bran, 5-10% of volcanic stone powder, 1-3% of magnesium sulfate, 0.5-1% of zinc sulfate, 0.5-1% of manganese sulfate, 0.1-0.2% of brown sugar and 0.1-0.2% of fermentation microorganisms, wherein the fermentation microorganisms are bacillus subtilis, bacillus licheniformis, rhizopus oryzae and trichoderma viride, and total number of viable bacteria is greater than 5X108cfu / g; fermented fertilizer further contains a functional microorganism with total number of viable bacteria being greater than 2X107cfu / g, and the functional microorganism is acinetobacter calcoaceticus. The bio-organic fertilizer can effectively prevent tomato autotoxicity and improve tomato quality and yield.

The invention relates to a conditioner for slowing down continuous cropping obstacles of ginger and an application method thereof. Raw materials of the conditioner comprise, by weight, 3-8% of phenylalanine, 0.5-1.5% of ferulic acid ester, 0.5-1.5% of caffeic acid, 0.5-1.5% of isoeugenol, 0.5-3% of salicylic acid, 0.5-3% of chitosan, 1-3% of borax, 2-5% of zinc sulfate, 2-5% of magnesium sulfate, 3-10% of ethanol, 1-4% of acetic anhydride and 60-85% of water. The conditioner has a simple preparation technology, is low-cost, is used to raise autotoxicity resistance of ginger plants and has good effects of conditioning growth, enhancing stress resistance and slowing down continuous cropping obstacles of ginger. In comparison with yield of a control group without the application of the conditioner, yield of a group with the application of the conditioner is increased by 10-20%.

The invention discloses a planting method for relieving watermelon successive cropping obstacles. Through a planting method of intercropping peanuts and watermelons, secreta of peanut roots has the effect of facilitating growth of watermelon seedlings, and the components, playing the facilitation role, in the secreta of the peanut roots are water-soluble organic substances and include an allelopathic substance triacontanol which plays a role of facilitating growth of the watermelons; after being thoroughly decomposed and fermented, branches and leaves of peanut saplings can be used as fertilizer, on one hand, resource recycling is achieved, and the cost is reduced; on the other hand, substances obtained by thoroughly decomposing the branches and leaves of the peanut saplings have relatively high allelopathic promotion effects on the growth of the watermelons, the watermelons are effectively prevented from autotoxicity, the watermelon successive cropping obstacles are relieved, and therefore ecological and economic benefits are improved.

The invention relates to a biological bacterial fertilizer starter for adjusting microecology of strawberry rhizosphere soil. The biological bacterial fertilizer starter is characterized by being a biological agent prepared from 40 to 60 parts of burkholderia, 20 to 30 parts of saccharomycetes and 20 to 30 parts of bacillus subtilis; a burkholderia strain was preserved in China General Microbiological Culture Collection Center located in No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City, Institute of Microbiology, Chinese Academy of Sciences on January 29th, 2018, with a preservation number of CGMCC No.15326. Bacterial fertilizer prepared from the starter has good strawberry rhizosphere colonizing capacity and is safe and reliable; in addition, the bacterial fertilizer has agood degradation effect on autotoxins in soil for continuously cropping strawberries, promotes the stress resistance of strawberry plants and improves the yield and the fruit quality of the strawberries. The preparation method has the advantages of simplicity, reasonable formula, stable effect and obvious effect of relieving the autotoxicity of the strawberries.

The invention discloses a method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil. The method comprises steps as follows: soil samples are collected and treated, 20 g of the treated soil samples are weighed, extracted with methanol and subjected to ultrasonic processing, and the allelopathic autotoxicity substances are separated with an high performance liquid chromatography method; the chromatographic conditions are as follows: the chromatographic instrument adopts Agilent 1260, the chromatographic column adopts specifications of AgilentZorbax SB-C18, 250mm*4.6mm and 5 mu m, the mobile phase A is an H3PO4 aqueous solution with mass fraction of 0.1%, the mobile phase B is methanol, the flow speed is 1.0ml / min, the column temperature is 25 DEG C, the detecting wavelength is 203 nm, and gradient elution is performed. An experiment proves that the maximum varieties of the allelopathic autotoxicity substances are obtained through extraction and separation with the method; the method is simple and convenient to implement, and disturbance and loss caused by an extraction solvent to target compounds in samples as a result of a complicated extraction technology in the sample extraction process are reduced.

The invention relates to a corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of the corn continuous-cropping obstacle restoration agent. The corn continuous-cropping obstacle restoration agent is characterized by being prepared from pseudomonas oryzihabitans and burkholderia according to a ratio of (1:3) to (3:1), the concentration of the pseudomonas oryzihabitans in a restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml, and the concentration of the burkholderia in the restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml. The application method of the restoration agent comprises the following steps: adopting a seed coating method, stirring and sufficiently mixing 50g of corn seeds and 5mL of fermented solution, standingfor 3 to 5 hours, and then seeding. The restoration agent adopted by the invention is pseudomonas oryzihabitans and burkholderia, and is good in corn rhizosphere fix planting capability, safe and reliable, good in degradation effect for an autotoxic substance in the continuous cropping corn soil, apparent in effect for promoting the stress resistance of the corn, and capable of increasing the yield of the corns and improving the fruit quality; and the restoration agent is simple in preparation method, reasonable in formula, and capable for controlling the corn continuous cropping obstacles.

The invention relates to a conditioner for slowing down continuous cropping obstacles of ginger and an application method thereof. Raw materials of the conditioner comprise, by weight, 3-8% of phenylalanine, 0.5-1.5% of ferulic acid ester, 0.5-1.5% of caffeic acid, 0.5-1.5% of isoeugenol, 0.5-3% of salicylic acid, 0.5-3% of chitosan, 1-3% of borax, 2-5% of zinc sulfate, 2-5% of magnesium sulfate, 3-10% of ethanol, 1-4% of acetic anhydride and 60-85% of water. The conditioner has a simple preparation technology, is low-cost, is used to raise autotoxicity resistance of ginger plants and has good effects of conditioning growth, enhancing stress resistance and slowing down continuous cropping obstacles of ginger. In comparison with yield of a control group without the application of the conditioner, yield of a group with the application of the conditioner is increased by 10-20%.

The invention discloses a root exudate collection system, comprising a liquid supply pool, a filtering system, a cultivation tank and a collection tank which are connected through a liquid supply system. Nutrient solution is stored in the liquid supply pool and supplied to the cultivation tank by a water pump. A liquid supply pipeline is arranged in the cultivation tank. Atomizing nozzles and a chromatographic column are arranged on the liquid supply pipeline. The invention further discloses a collection method using the root exudate collection system and a detection method for collected rootexudate. The collection and detection methods can be used to collect and detect root exudate in real time. The influence of other factors on the growth of crops is eliminated. The effect of root exudate on the growth of plants is judged by observing the growth condition of the crops in the cultivation tank, and the effect of relieving the autotoxicity of the crops by leaching the roots is verified.

The invention relates to a method for repairing continuous cropping obstacles of peanuts based on an allelopathic autotoxicity effect. The preparation method comprises the following steps: (1) seed dressing treatment is carried out by adopting a compound microbial agent in a peanut seeding period; the compound microbial agent comprises streptomyces tenebrarius and a synergist, wherein the synergist is a mixed solution of difenoconazole and a thiram solution diluted by 1000-2000 times, and the ratio of the difenoconazole to the thiram solution is 1:5-5:1; and the using method is seed dressing;and (2) a compound bacterial fertilizer is applied to holes in a peanut seedling period, and puddling is carried out; the bacterial fertilizer comprises 30-70 parts of sheep manure and 30-70 parts ofwormcast, and the usage amount of a microbial inoculum is 0.05-0.1%; and the microbial inoculum consists of 40-60 parts of streptomyces tenebrarius and 40-60 parts of yeast, and hole application treatment is carried out during seeding, 20-30 g of the compound bacterial fertilizer is applied to each hole, and the compound bacterial fertilizer is stirred and mixed with soil in the holes. The methodhas the beneficial effects that the emergence rate and the survival rate of peanuts are greatly improved. The sheep manure, the wormcast and the streptomyces tenebrarius are adopted for fermentation,so that the efficacy of the wormcast is supplemented by the sheep manure. The streptomyces tenebrarius is bred in the bacterial fertilizer, so that the production of a peanut root system is facilitated, and the influence on continuous cropping obstacles is eliminated.

The invention relates to a complex microbial inoculant used for eliminating the autotoxicity of corn and applications. The complex microbial inoculant is characterized by being composed of burkholderia phytofirmans and trichoderma at the ratio of (1:3) to (3:1), wherein burkholderia phytofirmans is preserved in the China General Microbiological Culture Collection Center (CGMCC, in the Institute ofMicrobiology, Chinese Academy of Sciences, located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing), and is assigned with the accession number of CGMCC No.15326, the strain number is20180101 Liao CK1, and the preservation date is Jan 29, 2018. According to the technical scheme, a seed coating method is adopted. The complex microbial inoculant is the mixture of plant endophyte and trichoderma probiotic, has good colonization capacity at the rhizosphere of corn plants, is safe and reliable, has the good degradation effect for autotoxins in the continuous cropping corn soil, obviously improves the physicochemical properties of the soil, has the obvious promoting effect for the anti-adversity capacity of the corn plants, and can improve the yield of the corn and the qualityof fruits. The complex microbial inoculant is simple in preparation method, is reasonable in formula, and can be used for treating the continuous cropping obstacles of corn.

The invention relates to a method for reducing allelopathy effect of Panax notoginseng, belonging to the technical field of planting Chinese medicinal materials. After the last crop of Panax notoginseng is harvested, the soil is leveled without any artificial chemical treatment: before ploughing, a base fertilizer and lime are evenly spread into the field soil and ploughed deeply. Ridging is thenperformed: conventional ridging, 16-17m in length and 1.9-2.0 m in width. Planting and management are performed by transplanting Panax notoginseng seedlings into ridges, planting 1-2 rows of Panax notoginseng every 1-2 rows, laying a mulch layer to form an intercropping system of Panax notoginseng vegetables, and then carrying out routine field management until Panax notoginseng matures. The method utilizes allelochemicals secreted by Panax notoginseng and vegetable roots and allelopathic effects to increase the emergence rate and survival rate of Panax notoginseng, reduce the effect of autotoxicity of Panax notoginseng on the growth and yield of Panax notoginseng, and effectively prevent the economic losses caused by diseases and pests through the intercropping of Panax notoginseng and vegetable.

The invention provides an extraction method of organic acid in asparagus rhizosphere soil. According to the technical scheme which is specific to the physical characteristics of soil and the ingredient characteristics of asparagus rhizosphere autotoxicity organic acid, an appropriate extraction scheme is designed. According to the method, the asparagus rhizosphere soil obtained via sampling and ultrapure water are mixed, soluble matter can be dissolved out from the soil by using a vibrating method, impurities are removed by using a filtration method, and obtained filtrate contains organic acid to be tested. On the basis, a cation exchange resin column and an anion exchange resin column are successively utilized by the method to perform chromatographic separation, the organic acid content to be tested in obtained eluate is fully promoted, finally, rotary evaporation condensation is conducted at relatively low temperature, a purified product is obtained on the basis of guaranteeing that an organic acid ingredient to be tested is stable, and the product can be used as a sample to be tested after being subjected to constant volume by using the ultrapure water so as to utilize HPLC (high performance liquid chromatography) to perform quantitative analysis.

The invention discloses a fertilizer special for capsicum annuum in the technical field of capsicum annuum planting. The fertilizer is prepared from, by weight, 38-46 parts of a BB fertilizer, 23-29 parts of sulfur coated urea, 35-47 parts of a potassium sulphate type compound fertilizer, 22-28 parts of humic acid, 7-10 parts of attapulgite powder and 0.2-0.5 part of EM bacteria. During preparation, the preparation method comprises the steps of firstly, grinding, wherein attapulgite is ground into the attapulgite powder with the specific surface area higher than 460 m<2> / kg; secondly, mixing,wherein the BB fertilizer, the sulfur coated urea, the potassium sulphate type compound fertilizer, the humic acid, the attapulgite powder and the EM bacteria are evenly mixed to prepare the fertilizer special for the capsicum annuum. In the scheme, the fertilizer special for the capsicum annuum achieves balanced supply of nutrients to capsicum annuum planting land through the interaction of the raw materials, the situation that a beneficial bacterium community is dominant is formed, and the autotoxicity, caused by allelochemicals, of the capsicum annuum is greatly reduced; therefore, the successive cropping obstacle of the capsicum annuum is greatly reduced.

The invention relates to a method for relieving pseudo-ginseng autotoxicity injuries by adding exogenous gentiobiose, belonging to the technical field of plant protection. The technical scheme is as follows: carrying out seed soaking treatment on pseudo-ginseng seeds by using a 1.0-50.0 mg / L gentiobiose solution; or dissolving gentiobiose in seed coating Celest to prepare a 5.0-500.0 mg / L gentiobiose seed coating, and carrying out coating treatment on the pseudo-ginseng seeds according to the agent:seed mass ratio of 1:50; or carrying out root soaking treatment on seeds and seedlings by using a 1.0-50.0 mg / L gentiobiose solution. The exogenous antioxidant is added to enhance the resistance of pseudo-ginseng, lower the cell injuries caused by autotoxic substances in the pseudo-ginseng growth process so as to increase the root rot damage probability, and reduce the consumption of pesticides; and thus, the method is an ecological safe effective measure for relieving successive cropping obstacles.

The invention relates to trichoderma atroviride C61, and belonging to the technical field of microorganisms. The trichoderma atroviride C61 is preserved in the China General Microbiological Culture Collection Center (CGMCC), No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China on December 8, 2020, with an accession number being CGMCC No. 21035. The invention also provides application of the trichoderma atroviride C61 in degradation of phenolic acids and derivatives thereof. The trichoderma atroviride C61 can degrade nine kinds of mixed phenolic acids with a concentration of 100 mg / L, wherein ferulic acid, benzoic acid, salicylic acid and cinnamic acid are completely degraded within 2 days; p-hydroxybenzoic acid, syringic acid and p-coumaric acid are completely degraded within 3 days; and vanillic acid and vanillic aldehyde are completely degraded within 6 days. Meanwhile, the trichoderma atroviride C61 can also effectively relieve autotoxicity effect of the phenolic acid and derivatives thereof on plants and repaire soil with continuous cropping obstacles.

The invention relates to a hydroponic device to study autotoxicity of muskmelons. The hydroponic device comprises a hydroponic box; a plurality of hydroponic chambers are arranged in an inner chamberof the hydroponic box; an orifice plate of the hydroponic box is provided with a plurality of groups of mounting through holes corresponding to the hydroponic chambers; fixing sponge for fixing seedlings of muskmelons is arranged in the mounting through holes; the roots of the seedlings of muskmelons extend into the hydroponic chambers. The invention also relates to an operating method of the hydroponic device. The hydroponic device is simple and reasonable in structural design, convenient to use, allows space to be effectively saved, ensures uniformity of experimental conditions, and allows development conditions of the seedlings of muskmelons under different combinations or single condition of autotoxicity of muskmelons and auto-toxicity to be conveniently observed.

The invention relates to a method for relieving pseudo-ginseng autotoxicity injuries by adding exogenous gentiobiose, belonging to the technical field of plant protection. The technical scheme is as follows: carrying out seed soaking treatment on pseudo-ginseng seeds by using a 1.0-50.0 mg / L gentiobiose solution; or dissolving gentiobiose in seed coating Celest to prepare a 5.0-500.0 mg / L gentiobiose seed coating, and carrying out coating treatment on the pseudo-ginseng seeds according to the agent:seed mass ratio of 1:50; or carrying out root soaking treatment on seeds and seedlings by using a 1.0-50.0 mg / L gentiobiose solution. The exogenous antioxidant is added to enhance the resistance of pseudo-ginseng, lower the cell injuries caused by autotoxic substances in the pseudo-ginseng growth process so as to increase the root rot damage probability, and reduce the consumption of pesticides; and thus, the method is an ecological safe effective measure for relieving successive cropping obstacles.

A soil conditioner for pepper planting land in the technical field of pepper planting disclosed in the scheme includes, by weight parts, 26-37 parts of pine bark, 73-95 parts of attapulgite clay, 0.8-1.2 parts of EM bacteria, 28-35 parts of fermented chaff, 23-37 parts of grass carbon, 37-52 parts of vinegar residue and 15-18 parts of tobaccos. During the preparation, a preparation method includesthe first step of placing the pine bark and the tobaccos in water for soaking and fermentation for 15-20 d; the second step of first wetting the chaff to a water content of 50-60%, and then applyinga layer of urea on each layer of chaff which is 8-12 cm thick, wherein the urea adding amount is 0.35-0.65% of the dry chaff; performing airtight fermentation for 60-70 d, to obtain fermented chaff; the third step of mixing the fermented chaff, the grass carbon, the vinegar residue, the attapulgite clay and the soaked pine bark and tobaccos to obtain a mixture, and sealing and fermenting the mixture for 15-20 d; and the fourth step of mixing the fermented mixture and the EM bacteria to obtain the soil conditioner for the pepper planting land. The soil conditioner in the scheme can remarkably make an improvement on the problems of autotoxicity caused by continuous pepper production and the deterioration of soil physical and chemical shape.

The invention relates to the technical field of planting and particularly relates to a method for ecological improvement of pinellia ternata cultivation soil, comprising the following steps of sputumculture and green manure planting. The method of the invention can effectively kill the pathogenic microorganisms produced in the continuous cropping of pinellia ternata, regulate the soil biocenosis,accelerate the material circulation in the soil, inhibit the propagation of the soil-borne pathogenic microorganisms in the subsequent planting, and degrade the autotoxicity generated in the growth of pinellia ternate, so as to accelerate the formation of benign soil ecology after the continuous cropping of pinellia ternata.

The invention discloses a special waxberry biological organic fertilizer which is prepared from the following raw materials in parts by weight: 50-70 parts of sheep manure, 2-5 parts of calcium superphosphate, 2-5 parts of ammonium sulfate, 2-3 parts of potassium permanganate, 2-3 parts of potassium chloride, 2-3 parts of borax, 2-5 parts of ammonium molybdate, 20-30 parts of rice straw, 10-20 parts of broccoli stems and leaves, 5-10 parts of humic acid and 2-6 parts of autotoxicity degradation bacteria, wherein the content of the autotoxicity degradation bacteria is 0.001-0.003 billion / g. Theinvention further discloses a preparation method and a fertilization method of the special waxberry biological organic fertilizer. By adopting the special waxberry biological organic fertilizer and the reasonable fertilization method disclosed by the invention, not only is weak waxberry tree vigor rapidly recovered, but also the quality of waxberries can be improved.

The invention relates to a method for repairing continuous cropping obstacles of peanuts based on an allelopathic autotoxicity effect. The preparation method comprises the following steps: (1) seed dressing treatment is carried out by adopting a compound microbial agent in a peanut seeding period; the compound microbial agent comprises streptomyces tenebrarius and a synergist, wherein the synergist is a mixed solution of difenoconazole and a thiram solution diluted by 1000-2000 times, and the ratio of the difenoconazole to the thiram solution is 1:5-5:1; and the using method is seed dressing;and (2) a compound bacterial fertilizer is applied to holes in a peanut seedling period, and puddling is carried out; the bacterial fertilizer comprises 30-70 parts of sheep manure and 30-70 parts ofwormcast, and the usage amount of a microbial inoculum is 0.05-0.1%; and the microbial inoculum consists of 40-60 parts of streptomyces tenebrarius and 40-60 parts of yeast, and hole application treatment is carried out during seeding, 20-30 g of the compound bacterial fertilizer is applied to each hole, and the compound bacterial fertilizer is stirred and mixed with soil in the holes. The methodhas the beneficial effects that the emergence rate and the survival rate of peanuts are greatly improved. The sheep manure, the wormcast and the streptomyces tenebrarius are adopted for fermentation,so that the efficacy of the wormcast is supplemented by the sheep manure. The streptomyces tenebrarius is bred in the bacterial fertilizer, so that the production of a peanut root system is facilitated, and the influence on continuous cropping obstacles is eliminated.

The invention discloses a separation, enrichment and detection method for a camptothecin-containing plant hydroponic root system allelochemical. The method comprises the following steps: firstly taking silica gel as a filler, filling a solid-phase extraction column, extracting and adsorbing root system metabolites for plant hydroponic solution, then taking out the filled silica gel, loading to an upper layer of a silica gel chromatography column, carrying out column chromatography isolation, then eluting in different combination ratios by taking petroleum ether, ethyl acetate, ethanol and chloroform as eluents, collecting eluate according to the composition of the eluate, carrying out rotary evaporation and concentration, and analyzing a separated component by utilizing a GC-MS technology. The method disclosed by the invention has the advantages that separation, enrichment and detection on a large number of allelochemicals in two kinds of plant hydroponic root secretions are realized; 25 allelochemicals are separated from camptotheca acuminate hydroponic root secretions and ophiorrhiza japonica hydroponic root secretions in total; and the method disclosed by the invention has very important technical guiding effect on ascertaining of more allelochemicals in a hydroponic system, substances with autotoxicity in the allelochemicals can be eliminated in time based on the method disclosed by the invention, plant growth cannot be inhibited, and vigorous growth of plants is promoted, so that the method disclosed by the invention has important significance on optimization of the plant hydroponic system.