21 results about "Sphingomonas" patented technology

The invention discloses a functional microbial agent for purifying breeding sewage and a preparation method of the functional microbial agent, and belongs to the technical field of functional microbial materials. The microbial agent is composed of a core functional unit and a porous carrier according to a mass ratio of 3: 1 to 5: 1, the core functional unit comprises a symbiotic engineering flora and a compound enzyme system in a volume ratio of 7: 1, and the symbiotic engineering flora is compounded by sphingomonas aeruginosa, paenibacillus splendidus and acinetobacter hemolyticus according to a ratio of 1: 1: 1; the porous carrier is a low-cost porous carbon material prepared on the basis of an animal waste blood / iron complex. Through systematic cooperation of a specific functional flora, a compound enzyme system and a special porous carrier, synergism of adsorption protection and biodegradation is achieved, the stress resistance and purification efficiency of the microbial agent in an antibiotic and heavy metal combined pollution environment are remarkably improved, and the industrial problem that an aquaculture sewage biological treatment system is prone to collapse is particularly solved; meanwhile, waste is treated by waste, and the method is low in cost and suitable for large-scale application.

The application belongs to the technical field of kidney disease differential diagnosis, and particularly relates to a microbial gene marker for differentiating diabetic nephropathy and membranous nephropathy and application thereof. The application provides sufficient evidence for the differentiation and treatment of clinical diagnosis of diabetic nephropathy and pathological diagnosis of membranous nephropathy based on four microbial markers of Blautia, Akkermansia, Sphingomonas and Granulicatella.

The invention discloses a sphingobacterium sp. And an application thereof. The sphingobacterium sp. Disclosed by the invention is preserved in the China Center for Type Culture Collection on May 7, 2025, and the preservation number of the sphingobacterium sp. Is CCTCC (China Center for Type Culture Collection) NO: M 2025987. The sphingobacterium sp. Disclosed by the invention can be used for treating and repairing polycyclic aromatic hydrocarbons (phenanthrene, fluoranthene and pyrene) in soil, water bodies or sediments under heavy metal stress, and has a relatively wide application prospect. The degradation rate of the sphingobacterium sp. To inorganic salt culture media containing 25 mg / L of phenanthrene, 25 mg / L of fluoranthene and 25 mg / L of pyrene can reach 80%-85% within 10 days, the tolerance of the sphingobacterium sp. To heavy metal is high, and the degradation rate range can reach 40%-85% under the condition that pollution of 0-100 mg / L of Cd < 2 + > exists at the same time.

This invention belongs to the field of environmental biotechnology, specifically, it relates to a prothioconazole methyltransferase gene. proS38 And its applications. This invention, through analysis, screening, identification, and verification, discovered... Sphingomonas Prothiozoxystrobin methyltransferase in sp. AJ-1 strain proS38 The gene and its protein expression product, prothioconazole methyltransferase, have been shown to have a highly efficient and specific degradation catalytic ability for the widely used pesticide prothioconazole, laying the foundation for the biodegradation of prothioconazole.

The invention discloses a bio-organic fertilizer taking edible fungus residues as a main material as well as a preparation method and application thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer taking the edible fungus residues as the main material is prepared from the following raw materials in parts by weight: 60 to 75 parts of the edible fungus residues, 15 to 25 parts of decomposed livestock and poultry manure, 5 to 8 parts of humic acid, 3 to 5 parts of a compound microbial agent, 0.5 to 1.5 parts of polyglutamic acid, 3 to 5 parts of a mineral activating agent, 2 to 4 parts of seaweed essence and 3 to 6 parts of functional biochar. Wherein the compound microbial agent is prepared from Granibacillus saccharophilus, Sphingobacterium aquaticum and Acinetobacter Guangzhou. The bio-organic fertilizer is obtained by screening functional microbial strains and compounding the functional microbial strains with other substances, resource utilization of edible fungus residue waste can be achieved, environmental pollution can be reduced, the physical and chemical properties of soil can be remarkably improved, the soil fertility can be improved, and the tomato yield and quality can be effectively improved.

A composition, preferably for caring for keratin materials, comprise: a) at least one microorganism of the genus Bifidobacterium species, the microorganism being in the lysate form, b) at least one Salvia miltiorrhiza extract, and c) Sphingomonas ferment extract. A non-therapeutic method for caring for the keratin materials comprises applying the composition to the keratin materials.

The application discloses a carbon-based microbial agent for synchronously degrading halogenated organic compounds and passivating heavy metals, and a preparation method and application thereof. The carbon-based microbial agent comprises composite microorganisms and biochar, the composite microorganisms comprise chitin bacteria, bacillus, achromobacter, methyl bacillus, sphingomonas and microbacterium, and the composite microorganisms are attached to the surface of the biochar. The preparation method of the carbon-based microbial agent is that six kinds of microbial strains are inoculated into culture media containing pollutants respectively, and after screening and domestication, the carbon-based microbial agent is obtained by incubating the six kinds of microbial strains with the biochar. The carbon-based microbial agent can synchronously degrade halogenated organic compounds and passivate heavy metals, the degradation rate of BDE-47 in actual electronic waste disassembly area composite contaminated soil can reach 89.60%, the degradation rate of PCB 180 can reach 94.99%, the passivation rates of lead and cadmium can reach 76.39% and 90.37% respectively, and the carbon-based microbial agent is suitable for heavy metal-organic compound composite contaminated site remediation.

This invention discloses a bacterial strain that degrades aspartame, a bacterial-algal symbiotic system, and its application. The strain is *Sphingosphomonas zeatus* (…). Sphingomonas zeae The specimen, NCU J1, with accession number CCTCC NO:M 20251462, was prepared using *Sphingomonas zeylans* and *Chlorella vulgaris*. Chlorella A directional symbiotic combination of *Sphingosine monocytogenes* sp. constructs a bacterial-algal symbiotic system. *Sphingosine monocytogenes* can metabolize aspartame and its degradation products, and can achieve metabolic complementarity through the bacterial-algal symbiotic interface. The bacteria preferentially promote the photohydrolysis of aspartame to generate degradation products such as diketopiperazine derivatives, while the microalgae simultaneously initiate isomerization enzymatic reactions to convert the degradation products into low-toxicity products. Compared with traditional physicochemical methods, this reduces the risk of secondary pollution by more than 40%, achieving efficient degradation of aspartame and significantly reducing the formation of its derivatives. It can be used to treat wastewater containing aspartame.

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS32 and application thereof. According to the invention, a key enzyme prothioconazole methyltransferase proS32 gene of the strain Sphingomonas sp. AJ-1 for degrading prothioconazole is found through biological information analysis and gene cloning, the prothioconazole methyltransferase ProS32 is obtained through expression of an expression vector, and the prothioconazole methyltransferase ProS32 is proved to have efficient and specific degradation catalysis capability on prothioconazole by utilizing HPLC (High Performance Liquid Chromatography) and enzymatic detection; and a foundation is laid for developing a corresponding biodegradation reagent or developing efficient degradation bacteria.

The present disclosure relates to a liposome composition including a culture extract of Sphingomonas olei and a cosmetic composition including the same. The liposomal composition according to the present disclosure encapsulates an extract of Sphingomonas olei culture broth in liposome, thereby efficiently delivering the culture extract of Sphingomonas olei into the skin and thus exhibiting enhanced skin regeneration efficacy and improved transdermal absorption.

The invention discloses a synthetic bacterial agent for inhibiting rhizosphere colonization of polymyxa cerealis serving as a wheat soil-borne virus transmission medium and application of the synthetic bacterial agent, and belongs to the technical field of environmental microorganism and plant disease prevention and control. The invention relates to a synthetic bacterial agent, which is characterized in that the synthetic bacterial agent is prepared from one or more of the following components: Massilla kyonggiensis, Paenibacillus silage, Paraburkholderia caledonica, Bacillus aryabhattai, Mycobacterium goodii and Sphingomonas azotifgens, and the synthetic bacterial agent is prepared from one or more of the following components: Sphingomonas kyonggiensis, Paenibacillus silage, Paraburkholderia caledonica, Bacillus aryabhattai, Mycobacterium goodii and Sphingomonas azotifgens. The synthetic bacterial agent disclosed by the invention is applied to plants, can obviously inhibit rhizosphere / root colonization of a wheat soil-borne virus transmission medium polymyxa graminearum, is safe, green and environment-friendly, and provides technical support for a green prevention and control technology of wheat virus diseases transmitted by the polymyxa graminearum.

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS18 and application thereof. According to the invention, a key enzyme prothioconazole methyltransferase proS18 gene of the strain Sphingomonas sp. AJ-1 for degrading prothioconazole is found through biological information analysis and gene cloning, the prothioconazole methyltransferase ProS18 is obtained through expression of an expression vector, and the prothioconazole methyltransferase ProS18 is proved to have efficient and specific degradation catalysis capability on prothioconazole by utilizing HPLC (High Performance Liquid Chromatography) and enzymatic detection; and a foundation is laid for developing a corresponding biodegradation reagent or developing efficient degradation bacteria.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS18 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS18 The gene was obtained and prothioconazole methyltransferase ProS18 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

The invention discloses an ecological bottom mud repair material and a preparation method thereof, and relates to the technical field of water environment governance, the ecological bottom mud repair material comprises the following components by mass: 70-85 parts of a modified matrix material, 5-10 parts of a multifunctional microbial liquid, and 5-25 parts of a shell material; the multifunctional microbial bacterial liquid comprises one or more of a bacillus bacterial liquid, a pseudomonas bacterial liquid and a sphingomonas bacterial liquid; the shell material comprises one or more of diatomite, bentonite and zeolite powder. According to the ecological bottom mud remediation material, functional microorganisms form an adsorption-colonization-metabolism continuous action chain in bottom mud, the removal efficiency of total phosphorus and total nitrogen in the bottom mud reaches 25.2% and 35% respectively, and the material is environmentally friendly to a water body and the bottom mud and is completely mineralized in the remediation process, so that secondary pollution is avoided.

The invention discloses self-repairing concrete based on a gellan gum-microorganism composite carrier as well as a preparation method and application of the self-repairing concrete. Relates to the concrete field. The concrete comprises a concrete base material and a gellan gum-microorganism composite carrier dispersed in the concrete base material, the gellan gum-microorganism composite carrier comprises gellan gum, urease-producing bacteria, calcium salt and a calcium-containing nutrient source. The gellan gum is used for replacing a traditional carrier (such as sodium alginate and epoxy resin), the gellan gum is anionic polysaccharide generated by fermentation of sphingomonas paucimobilis, a molecular chain contains carboxylic acid groups, and the gellan gum is stable in the pH range of 3-13, so that the gellan gum-microorganism composite carrier is prevented from being degraded due to alkali corrosion, and long-term protection is provided for microorganisms. In addition, the gellan gum is high in gel strength and can resist shear stress in the concrete stirring process, and the situation that microorganisms are released too early due to the fact that the gellan gum-microorganism composite carrier is broken in advance is avoided.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS32 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS32 The gene was obtained and prothioconazole methyltransferase ProS32 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

This invention belongs to the field of enzyme engineering technology, specifically relating to proteins with tetrahydrofolate-dependent demethylase activity and their application in the synthesis of L-5-methyltetrahydrofolate. This invention utilizes two proteins with tetrahydrofolate-dependent demethylase activity, namely *Sphingosine monocytogenes* (…). Sphingomonas paucimobilis Vanillic acid O-demethylase and Amorpha fruticosa intermediate rhizobia (from) Mesorhizobium amorphae The aminomethyltransferase derived from 6S-THF uses inexpensive vanillic acid or dicamba as methyl donors to directly methylate 6S-THF to synthesize L-5-MTHF. Combined with highly active dihydrofolate reductase and NADP cofactor regeneration system, this method effectively achieves efficient and green synthesis of L-5-methyltetrahydrofolate, and has good industrialization prospects.

The invention discloses a method for repairing petroleum-contaminated soil through combination of a surfactant and biological enhancement, and belongs to the field of petroleum-contaminated soil repairing. The method comprises the following steps: adding an alkyl glycoside surfactant into the petroleum-contaminated soil to carry out efficient surfactant leaching on the soil, and then adding sphingomonas into the soil to carry out biological enhancement remediation on the soil. By means of the remediation process, humic acid substances in the soil are remarkably increased, and by means of treatment in the process, the solubility of TPH is improved, and the accessibility of microorganisms to the TPH is enhanced; in the bioremediation stage, the efficient surfactant can serve as a stronger additional carbon source, the number and activity of microorganisms are improved, and generation of more soil enzymes is promoted, so that the whole remediation process is accelerated, and the purpose of bioremediation of petroleum hydrocarbon in soil through combination of the surfactant and the bioremediation is achieved.

The invention relates to a sunscreen composition and a preparation method thereof. The sunscreen composition comprises the following components in percentage by mass: 0.08-2% of a thickening agent, 3-6% of an emulsifier, 6-12% of a humectant, 13-20% of a skin feeling regulator, 20-24% of a physical sunscreen agent and the balance of water, wherein the thickening agent is selected from a mixture of a polyacrylate cross-linked polymer-6, xanthan gum and / or dehydrogenated xanthan gum, and a sphingomonas sphingomonas fermentation product extract product. The sunscreen composition prepared by the invention not only has an excellent sunscreen effect, but also is uniform in material body, fine and smooth in texture and good in stability, a physical sunscreen agent is not aggregated and settled, the sunscreen composition has good spreadability, freshness and soft skin feeling, the content of natural source components of the sunscreen composition reaches 90% or above, and the sunscreen composition does not need to use organic silicone oil, is mild and non-irritating, is suitable for children, and has a good application prospect. And pregnant women and sensitive skin.

The present application belongs to the field of agricultural and environmental microbial technology, and particularly relates to a sphingopyxis sp. for blocking and controlling plant absorption of heavy metals, a bacterial agent, a preparation method and application thereof. The sphingopyxis sp. is named YHY20-d, and was preserved in the China General Microbiological Culture Collection Center on December 30, 2024, with a preservation number of CGMCC NO.33226. Inoculation of the sphingopyxis sp. YHY20-d in heavy metal contaminated soil can reduce the content of available heavy metals in the soil, effectively block and control plant absorption of heavy metals such as cadmium, lead and copper, increase the content of organic matter and available phosphorus in the rhizosphere soil of the plant, promote the transformation of ammonium nitrogen to nitrate nitrogen in the soil, and improve the ecological environment of the soil.