5 results about "Sphingomonas" patented technology

This invention belongs to the field of environmental biotechnology, specifically, it relates to a prothioconazole methyltransferase gene. proS38 And its applications. This invention, through analysis, screening, identification, and verification, discovered... Sphingomonas Prothiozoxystrobin methyltransferase in sp. AJ-1 strain proS38 The gene and its protein expression product, prothioconazole methyltransferase, have been shown to have a highly efficient and specific degradation catalytic ability for the widely used pesticide prothioconazole, laying the foundation for the biodegradation of prothioconazole.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS18 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS18 The gene was obtained and prothioconazole methyltransferase ProS18 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS32 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS32 The gene was obtained and prothioconazole methyltransferase ProS32 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

This invention belongs to the field of enzyme engineering technology, specifically relating to proteins with tetrahydrofolate-dependent demethylase activity and their application in the synthesis of L-5-methyltetrahydrofolate. This invention utilizes two proteins with tetrahydrofolate-dependent demethylase activity, namely *Sphingosine monocytogenes* (…). Sphingomonas paucimobilis Vanillic acid O-demethylase and Amorpha fruticosa intermediate rhizobia (from) Mesorhizobium amorphae The aminomethyltransferase derived from 6S-THF uses inexpensive vanillic acid or dicamba as methyl donors to directly methylate 6S-THF to synthesize L-5-MTHF. Combined with highly active dihydrofolate reductase and NADP cofactor regeneration system, this method effectively achieves efficient and green synthesis of L-5-methyltetrahydrofolate, and has good industrialization prospects.